CN111621423A - High-phosphorus-loading living microalgae and preparation method and application thereof - Google Patents
High-phosphorus-loading living microalgae and preparation method and application thereof Download PDFInfo
- Publication number
- CN111621423A CN111621423A CN202010462294.5A CN202010462294A CN111621423A CN 111621423 A CN111621423 A CN 111621423A CN 202010462294 A CN202010462294 A CN 202010462294A CN 111621423 A CN111621423 A CN 111621423A
- Authority
- CN
- China
- Prior art keywords
- phosphorus
- microalgae
- wastewater
- living
- living microalgae
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000011068 loading method Methods 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 49
- 239000011574 phosphorus Substances 0.000 claims abstract description 49
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 47
- 239000002351 wastewater Substances 0.000 claims abstract description 45
- 238000001179 sorption measurement Methods 0.000 claims abstract description 35
- 229910001385 heavy metal Inorganic materials 0.000 claims abstract description 34
- 239000001963 growth medium Substances 0.000 claims abstract description 8
- 241000195493 Cryptophyta Species 0.000 claims description 26
- 238000000034 method Methods 0.000 claims description 19
- 239000002028 Biomass Substances 0.000 claims description 15
- 150000002500 ions Chemical class 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 3
- 241000195628 Chlorophyta Species 0.000 claims description 2
- 241000168525 Haematococcus Species 0.000 claims description 2
- 241000192701 Microcystis Species 0.000 claims description 2
- 241000195663 Scenedesmus Species 0.000 claims description 2
- 241000192584 Synechocystis Species 0.000 claims description 2
- 230000004913 activation Effects 0.000 claims description 2
- 239000002609 medium Substances 0.000 claims description 2
- 229910052793 cadmium Inorganic materials 0.000 abstract description 15
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 10
- 238000012851 eutrophication Methods 0.000 abstract description 3
- 230000010355 oscillation Effects 0.000 abstract description 2
- 239000002352 surface water Substances 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 9
- -1 amino, carboxyl Chemical group 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- YKYOUMDCQGMQQO-UHFFFAOYSA-L cadmium dichloride Chemical compound Cl[Cd]Cl YKYOUMDCQGMQQO-UHFFFAOYSA-L 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 229910021645 metal ion Inorganic materials 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 125000000524 functional group Chemical group 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 241001036355 Didymogenes palatina Species 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
- 238000002798 spectrophotometry method Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 229910021380 Manganese Chloride Inorganic materials 0.000 description 1
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- FRHBOQMZUOWXQL-UHFFFAOYSA-L ammonium ferric citrate Chemical compound [NH4+].[Fe+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O FRHBOQMZUOWXQL-UHFFFAOYSA-L 0.000 description 1
- 239000011609 ammonium molybdate Substances 0.000 description 1
- 235000018660 ammonium molybdate Nutrition 0.000 description 1
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 description 1
- 229940010552 ammonium molybdate Drugs 0.000 description 1
- 238000002306 biochemical method Methods 0.000 description 1
- 229940065285 cadmium compound Drugs 0.000 description 1
- 150000001662 cadmium compounds Chemical class 0.000 description 1
- WLZRMCYVCSSEQC-UHFFFAOYSA-N cadmium(2+) Chemical compound [Cd+2] WLZRMCYVCSSEQC-UHFFFAOYSA-N 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 231100000739 chronic poisoning Toxicity 0.000 description 1
- 238000010668 complexation reaction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 229960004642 ferric ammonium citrate Drugs 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 231100000171 higher toxicity Toxicity 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-M hydrosulfide Chemical compound [SH-] RWSOTUBLDIXVET-UHFFFAOYSA-M 0.000 description 1
- 239000004313 iron ammonium citrate Substances 0.000 description 1
- 235000000011 iron ammonium citrate Nutrition 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000011565 manganese chloride Substances 0.000 description 1
- 238000011177 media preparation Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- KEORAKCBDAHFDX-UHFFFAOYSA-N triazanium 2-hydroxypropane-1,2,3-tricarboxylate hydrate Chemical compound [NH4+].[NH4+].[NH4+].O.OC(CC([O-])=O)(CC([O-])=O)C([O-])=O KEORAKCBDAHFDX-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Botany (AREA)
- Water Supply & Treatment (AREA)
- Environmental & Geological Engineering (AREA)
- General Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Virology (AREA)
- Ecology (AREA)
- Cell Biology (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a high-phosphorus-loading living microalgae and a preparation method and application thereof, wherein the preparation method comprises the following steps: and (3) carrying out activated culture on the microalgae in a phosphorus-containing culture medium to obtain the high-phosphorus-loading living microalgae. The invention utilizes phosphorus element to modify active microalgae, and the obtained high-phosphorus-carrying living microalgae can greatly improve the adsorption capacity of the active microalgae to heavy metals, when the high-phosphorus-carrying living microalgae is used for treating the heavy metals in wastewater, the heavy metals in the wastewater can be effectively removed only by simple oscillation adsorption, wherein the adsorption capacity of the high-phosphorus-carrying living microalgae to cadmium in the wastewater can reach 7.41mg/g, and the high-phosphorus-carrying living microalgae can promote the growth of the microalgae and simultaneously reduce the pollution of phosphorus to a water body, and can reduce the total phosphorus concentration in the wastewater to be below 0.2mg/L, so that the high-phosphorus-carrying living microalgae can reach the III-type standard discharge of surface water environment quality, avoid the eutrophication of the water body caused by excessive phosphorus element in the water body, and further realize the purposes of removing the heavy metals and phosphorus by the microalgae and treating the wastewater.
Description
Technical Field
The invention relates to the technical field of environmental engineering, in particular to high-phosphorus-loading living microalgae and a preparation method and application thereof.
Background
Phosphorus is a source causing water eutrophication, and is also a necessary nutrient substance for the growth of active microalgae, and the conventional treatment method of phosphorus-containing wastewater mainly comprises a biochemical method, a physical adsorption method and a chemical phosphorus removal method, and the methods generally have the problems of high cost, unsatisfactory phosphorus removal effect and the like.
The waste water containing cadmium has high toxicity, and the cadmium compound has higher toxicity. Cadmium ions are easily enriched in organisms, for example, cadmium-containing wastewater is easy to cause chronic poisoning of organisms without strict treatment, and the cadmium-containing wastewater has great harm. Cadmium chloride is the most toxic, and when the mass concentration is 0.001mg/L, the cadmium chloride can kill fishes and aquatic organisms. Cadmium can seriously inhibit the growth of microorganisms, and the death rate of the microorganisms can reach about 50% when the concentration is 0.1-1.0 mg/L. When the mass concentration of cadmium in the water is 0.1mg/L, the self-purification effect of the water body can be inhibited. Cadmium is a first pollutant in the clear stipulation of the Integrated wastewater discharge Standard (GB 8978-1996) of China, the maximum allowable discharge mass concentration is 0.1mg/L, and the cadmium cannot be diluted for treatment. Therefore, it is very important to treat the cadmium-containing wastewater.
The active microalgae contains abundant active functional groups on the surface, mainly comprises amino, carboxyl, sulfydryl, phosphate and hydroxyl, and has certain adsorption capacity on heavy metals while growing by using phosphorus elements. The mechanism of heavy metal ion adsorption by active microalgae is as follows: the combination of heavy metal ions and microalgae cells is divided into two steps. Firstly, the surface complexation of the cell wall and the metal ions, secondly, the metal ions enter the cells and are absorbed and internalized by the cells, and the harmful metal ions are converted into a non-toxic or low-toxic form through the detoxification mechanism in the cells. The method for treating the wastewater containing the heavy metals by using the active microalgae, namely the biomass method, has the advantages of low treatment price, simple and convenient operation and low energy consumption, however, the method is rarely researched at present, and the treatment effect is not ideal when the heavy metals are treated by simply using the active microalgae. Therefore, the invention adopts phosphorus to modify the live algae, and greatly enhances the tolerance and the adsorption capacity of the live algae to heavy metals.
Disclosure of Invention
In view of the above, the invention aims to provide a preparation method of high-phosphorus-loading living microalgae, so as to solve the problems of high treatment cost and unsatisfactory treatment effect of heavy metals in the existing heavy metal-containing wastewater.
In order to achieve the purpose, the technical scheme of the invention is realized as follows:
a preparation method of high-phosphorus-loading living microalgae comprises the following steps:
and (3) carrying out activated culture on the microalgae in a phosphorus-containing culture medium to obtain the high-phosphorus-loading living microalgae.
Optionally, the concentration of phosphorus in the phosphorus-containing medium is no more than 200mg/L as P.
Optionally, the microalgae is one or more of aquatic unicellular green algae and blue algae.
Optionally, the microalgae is one or more of chlorella, scenedesmus, chlorella, microcystis, synechocystis, and haematococcus.
Optionally, the activation culture is carried out for 8-12 days at 25-40 deg.C under alternate light-irradiation and non-light-irradiation conditions.
The second purpose of the invention is to provide high-phosphorus-loading living microalgae, which is prepared by the preparation method of the high-phosphorus-loading living microalgae.
The third purpose of the invention is to provide an application of the high-phosphorus-loading living microalgae in treating heavy metal-containing wastewater, which comprises the following steps:
and mixing the high-phosphorus-loading living microalgae with the wastewater containing heavy metals, performing biological adsorption, and separating after the biological adsorption is finished to obtain the biomass containing heavy metals and the wastewater without heavy metals.
Optionally, the concentration of heavy metal ions in the heavy metal wastewater is 10-30 mg/L.
Optionally, the adsorption temperature of the bioadsorption is 25-30 ℃ and the adsorption pH is 3-6.
The fourth purpose of the invention is to provide an application of the high-phosphorus-loading living microalgae in treating phosphorus-containing wastewater, which comprises the following steps:
mixing the high-phosphorus-loading living microalgae with the phosphorus-containing wastewater according to the mass ratio of 1: 100-1000, performing biological adsorption, and separating after the biological adsorption is finished to obtain the phosphorus-containing biomass and the dephosphorization wastewater.
Compared with the prior art, the preparation method of the high-phosphorus-loading living microalgae has the following advantages:
1. the invention utilizes phosphorus element to modify active microalgae, and the obtained high-phosphorus-carrying living microalgae can greatly improve the adsorption capacity of the active microalgae to heavy metals, when the high-phosphorus-carrying living microalgae is used for treating the heavy metals in wastewater, the heavy metals in the wastewater can be effectively removed only by simple oscillation adsorption, wherein the adsorption capacity of the high-phosphorus-carrying living microalgae to cadmium in the wastewater can reach 7.41mg/g, and the high-phosphorus-carrying living microalgae can promote the growth of the microalgae and simultaneously reduce the pollution of phosphorus to a water body, and can reduce the total phosphorus concentration in the wastewater to be below 0.2mg/L, so that the high-phosphorus-carrying living microalgae can reach the III-type standard discharge of surface water environment quality, avoid the eutrophication of the water body caused by excessive phosphorus element in the water body, and further realize the purposes of removing the heavy metals and phosphorus by the microalgae and treating the wastewater.
2. When the high-phosphorus-loading living microalgae is used for carrying out an adsorption experiment, the high-phosphorus-loading living microalgae can be carried out in a liquid form, compared with algae powder, the method saves the treatment steps of centrifugation, drying, freeze-drying and the like, greatly simplifies the adsorption step, and has the advantages of wide raw material source, low price and easiness in industrial popularization and application.
3. Compared with some fungi (1.87 mmol/g adsorption capacity), the adsorption capacity of the live algae is higher, the microalgae is better cultured and stocked than bacteria, and the method is more suitable for engineering examples.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate an embodiment of the invention and, together with the description, serve to explain the invention and not to limit the invention. In the drawings:
FIG. 1 is a graph showing the growth of living microalgae and the change of phosphorus concentration in the culture medium according to the present invention;
FIG. 2 is a relation curve of adsorption capacity of modified biomass (high-phosphorus-loading living microalgae) with different phosphorus concentrations to Cd (II) with pH change at room temperature;
FIG. 3 is a relation curve of the adsorption capacity of the modified biomass (high-phosphorus-loading living microalgae) with different phosphorus concentrations to Cd (II) with the change of initial concentration at room temperature.
Detailed Description
It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.
The present invention will be described in detail below with reference to the drawings and examples.
The microalgae used in the embodiment of the invention is particularly Didymogenespalatina XR which is separated and purified from a mine soil sample according to a conventional method, and the biomass of the microalgae is characterized by rapid growth and high lipid content. The surface of the DidymogenespalatinaXR algae is rich in various active functional groups, mainly comprising amino, carboxyl, hydrosulfide, phosphate and hydroxyl, and the active functional groups provide binding sites for metal ions to be adsorbed on the surface of the algae. Phosphorus-containing wastewater used in the examples of the present invention was K2HPO4The cadmium-containing wastewater is prepared according to the concentration of P, and the used cadmium-containing wastewater is prepared by diluting cadmium standard solution (1mg/mL) purchased from Aladdin company to a specified concentration.
Example 1
In the embodiment, culture media with different phosphorus concentrations are adopted to perform phosphorus modification on microalgae to obtain different series of high-phosphorus-loading living microalgae, and the method specifically comprises the following steps:
1) BG-11 culture medium preparation: 1.5g NaNO3，K2HPO4(the adding amount is changed according to different modified media), 36mgCaCl2·2H2O, 6mg ammonium citrate-water, 6mg ferric ammonium citrate, 1mg EDTA, 2.86. mu.g H3BO3，1.81μg MnCl2·4H2O,0.222μgZnSO4·7H2O，0.39μgNaMoO4·5H2O，0.079μgCuSO 4·5H2O，0.050μgCoCl2·6H2O, 1L of sterile distilled water;
2) activating and modifying microalgae: inoculating the DidymogenespalatinaXR microalgae into culture medium with phosphorus concentration (calculated as P) of 40, 80, 160 and 200mg/L at a concentration of 0.08-0.1g/L, and irradiating at 25 deg.C and 3600LX with light intensity and light-dark ratio of 14 h: culturing for 10h under the environment, sampling and measuring the phosphorus concentration in the solution (sampling every 48h, measuring the phosphorus concentration by adopting ammonium molybdate ultraviolet spectrophotometry at the wavelength of 700 nm) and the biomass of the live algae (sampling every 48h, measuring the live algae concentration at the wavelength of 680nm by adopting an ultraviolet spectrophotometry), analyzing according to a biomass curve, stopping aeration after the live algae grows to a stable period (generally to 8 days), and storing at the condition of 4 ℃ to obtain modified fresh algae liquid, namely high-phosphorus-loaded living microalgae, wherein the high-phosphorus-loaded living microalgae cultured in different culture media are named as B-40, B-80, B-160 and B-200.
The relationship between the growth biomass and the growth days of the modified live algae cultured at different phosphorus concentrations and the relationship between the P content in the culture solution and the time were tested, and the test results are shown in FIG. 1, in which FIG. 1(a) is a graph of the growth biomass and the growth days of the modified live algae cultured at different phosphorus concentrations, and FIG. 1(b) is a graph of the P content in the culture solution and the time.
As shown in FIG. 1, Didymogenespalatina XR microalgae reached a stable state around day 8 of growth, and the biomass of the microalgae decreased thereafter, so the experiments were performed using microalgae grown up to day 8, and the microalgae had the best efficiency of absorbing and utilizing phosphorus in the culture solution.
Example 2
The modified live algae, namely the high-phosphorus-loading living microalgae prepared in the embodiment 1 of the invention is used for treating heavy metal-containing wastewater, wherein the heavy metal-containing wastewater is Cd (II) -containing wastewater, and the method specifically comprises the following steps:
1) calculating the biological concentration of each modified live alga, namely four live alga (B-40, B-80, B-160 and B-200) in example 1, and then respectively taking 5mg of the four live alga (B-40, B-80, B-160 and B-200) in example 1 into a centrifuge tube (according to the liquid volume);
2) adding 8mL of Cd (II) solution with the initial concentration of 2mg/L into a centrifugal tube containing 5mg of the algae sample, adjusting the pH value of the solution to 3.0, 4.0, 5.0 and 6.0, oscillating and adsorbing for 1.5 hours on a vibration table at the temperature of 25 ℃ and the rotating speed of 150rad/min, and then carrying out centrifugal separation on the adsorbed algae solution to obtain Cd (II) -containing biomass and Cd (II) -removing wastewater (supernatant).
In this example, the concentration of Cd (ii) in the supernatant was determined by flame atomic absorption, three replicates of each group of live algae were taken, and the experimental results averaged; the content of Cd (II) in the cadmium-containing wastewater adsorbed by the four modified live algae in example 1 at different pH values (3.0, 4.0, 5.0 and 6.0) was respectively tested, and an adsorption histogram of the removal rate of Cd (II) by the modified live algae was prepared, as shown in FIG. 2.
As can be seen from fig. 2, each modified active microalgae in example 1 of the present invention has a high removal rate for Cd (ii), wherein the removal rate for Cd (ii) by each modified active microalgae is the maximum when the pH is 6.
Example 3
The modified live algae, namely the high-phosphorus-loading living microalgae prepared in the embodiment 1 of the invention is used for treating heavy metal-containing wastewater, wherein the heavy metal-containing wastewater is Cd (II) -containing wastewater, and the method specifically comprises the following steps:
1) respectively taking 5mg of the four live algae (B-40, B-80, B-160 and B-200) in the example 1 in a centrifuge tube (according to the liquid volume);
2) adding 8mL of Cd (II) solution with the concentration gradient of 2-30 mg/L into a centrifuge tube containing the four living algae, adjusting the pH value of the solution to 6.0, oscillating and adsorbing for 1.5 hours on a vibration table at the temperature of 25 ℃ and the rotating speed of 150rad/min, and then carrying out centrifugal separation on the algae liquid after adsorption to obtain the biomass containing Cd (II) and the wastewater (supernatant) without Cd (II).
In this embodiment, the concentration of Cd (ii) in the supernatant is determined by a flame atomic absorption method, three parallel samples are taken from each group of live microalgae, the experimental results are averaged, the obtained results are used as adsorption isotherms, and Langmuir, Freundlich and Temkin isotherm fitting is performed on each modified microalgae to obtain the adsorption condition of each modified microalgae to Cd (ii), and the test results are shown in fig. 3 and table 1.
As can be seen from FIG. 3, the adsorption of each modified microalgae in example 1 of the present invention meets Langmuir adsorption kinetics standards, the optimum phosphorus concentration is determined according to the maximum adsorption amount obtained from the fitting result, and the adsorption equilibrium concentration of B-160 is the highest and reaches 7.41 mg/g.
As can be seen from Table 1, the B-160 algae modified by phosphorus has the best effect of adsorbing cadmium, and the adsorption of heavy metals by algae more conforms to the Langmuir model.
TABLE 1
The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions, improvements, etc. within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A preparation method of high-phosphorus-loading living microalgae is characterized by comprising the following steps:
and (3) carrying out activated culture on the microalgae in a phosphorus-containing culture medium to obtain the high-phosphorus-loading living microalgae.
2. The method for preparing living microalgae with high phosphorus content according to claim 1, wherein the concentration of phosphorus in the phosphorus-containing medium is not more than 200mg/L in terms of P.
3. The method for preparing high phosphorus-carrying live microalgae according to claim 1, wherein the microalgae is one or more of aquatic unicellular green algae and blue algae.
4. The method for preparing high phosphorus content living microalgae according to claim 3, wherein the microalgae is one or more of Chlorella, Scenedesmus, Chlorococcus, Microcystis, Synechocystis, Haematococcus.
5. The method for preparing living microalgae with high phosphorus content as claimed in claim 1, wherein the activation culture is carried out for 8-12 days at 25-40 deg.C under an alternate light-to-no light environment.
6. A high phosphorus-carrying living microalgae, which is prepared by the method for preparing the high phosphorus-carrying living microalgae according to any one of claims 1 to 5.
7. The application of the high-phosphorus-loading living microalgae for treating heavy metal-containing wastewater in the claim 6 is characterized by comprising the following steps:
and mixing the high-phosphorus-loading living microalgae with the wastewater containing heavy metals, performing biological adsorption, and separating after the biological adsorption is finished to obtain the biomass containing heavy metals and the wastewater without heavy metals.
8. The application of the high-phosphorus-carrying living microalgae according to claim 7 in treating heavy metals in wastewater, wherein the concentration of heavy metal ions in the heavy metal wastewater is 10-30 mg/L.
9. The method for preparing living microalgae with high phosphorus loading capacity as claimed in claim 7, wherein the temperature of the bioadsorption is 25-30 ℃ and the pH of the bioadsorption is 3-6.
10. The application of the high-phosphorus-loading living microalgae in treating phosphorus-containing wastewater in claim 6 is characterized by comprising the following steps:
mixing the high-phosphorus-loading living microalgae with the phosphorus-containing wastewater according to the mass ratio of 1: 100-1000, performing biological adsorption, and separating after the biological adsorption is finished to obtain the phosphorus-containing biomass and the dephosphorization wastewater.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010462294.5A CN111621423A (en) | 2020-05-27 | 2020-05-27 | High-phosphorus-loading living microalgae and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010462294.5A CN111621423A (en) | 2020-05-27 | 2020-05-27 | High-phosphorus-loading living microalgae and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111621423A true CN111621423A (en) | 2020-09-04 |
Family
ID=72270220
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010462294.5A Pending CN111621423A (en) | 2020-05-27 | 2020-05-27 | High-phosphorus-loading living microalgae and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111621423A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112547784A (en) * | 2020-11-10 | 2021-03-26 | 武汉理工大学 | Method for repairing heavy metal contaminated soil by high-phosphorus-loading microalgae passivator |
| CN114105307A (en) * | 2021-10-21 | 2022-03-01 | 太原理工大学 | Method for removing Cr in wastewater by using living heterotrophic microalgae6+Method (2) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107262039A (en) * | 2017-08-10 | 2017-10-20 | 福州大学 | A kind of high-molecular biologic charcoal ball immobilized microalgae compound adsorbent and its preparation and application |
| CN108587915A (en) * | 2018-05-16 | 2018-09-28 | 中国科学院水生生物研究所 | Chlorella W5 and the application of the heavy metal in high content of beary metal water body can be removed |
| CN109576159A (en) * | 2017-09-29 | 2019-04-05 | 武汉藻优生物科技有限公司 | A kind of energy removes chlorella W4 and its application of the heavy metal in high content of beary metal water body |
| CN109574235A (en) * | 2018-12-25 | 2019-04-05 | 武汉理工大学 | Utilize microalgae while the method for Removal of Phosphorus in Wastewater and mercury-containing waste water |
| CN109626582A (en) * | 2018-12-25 | 2019-04-16 | 武汉理工大学 | The method for removing lead in water removal using the modified microalgae of high phosphorus culture |
-
2020
- 2020-05-27 CN CN202010462294.5A patent/CN111621423A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107262039A (en) * | 2017-08-10 | 2017-10-20 | 福州大学 | A kind of high-molecular biologic charcoal ball immobilized microalgae compound adsorbent and its preparation and application |
| CN109576159A (en) * | 2017-09-29 | 2019-04-05 | 武汉藻优生物科技有限公司 | A kind of energy removes chlorella W4 and its application of the heavy metal in high content of beary metal water body |
| CN108587915A (en) * | 2018-05-16 | 2018-09-28 | 中国科学院水生生物研究所 | Chlorella W5 and the application of the heavy metal in high content of beary metal water body can be removed |
| CN109574235A (en) * | 2018-12-25 | 2019-04-05 | 武汉理工大学 | Utilize microalgae while the method for Removal of Phosphorus in Wastewater and mercury-containing waste water |
| CN109626582A (en) * | 2018-12-25 | 2019-04-16 | 武汉理工大学 | The method for removing lead in water removal using the modified microalgae of high phosphorus culture |
Non-Patent Citations (3)
| Title |
|---|
| RI-QING YU ET AL.,: ""Biological uptake of Cd, Se(IV) and Zn by Chlamydomonas reinhardtii in response to different phosphate and nitrate additions"", 《AQUATIC MICROBIAL ECOLOGY AQUAT MICROB ECOL》 * |
| RUSSELL S. WALSH ET AL.,: ""Influence of phosphorus storage on the uptake of cadmium by the marine alga Macrocystis pyrifera"", 《LIMNOL.OCEANOGR》 * |
| YINTA LI ET AL.,: ""Enhanced Pb(II) removal by algal-based biosorbent cultivated in high-phosphorus cultures"", 《CHEMICAL ENGINEERING JOURNAL》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112547784A (en) * | 2020-11-10 | 2021-03-26 | 武汉理工大学 | Method for repairing heavy metal contaminated soil by high-phosphorus-loading microalgae passivator |
| CN114105307A (en) * | 2021-10-21 | 2022-03-01 | 太原理工大学 | Method for removing Cr in wastewater by using living heterotrophic microalgae6+Method (2) |
| CN114105307B (en) * | 2021-10-21 | 2024-01-23 | 太原理工大学 | Cr in wastewater is removed by utilizing living heterotrophic microalgae 6+ Is a method of (2) |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zhuang et al. | Non-suspended microalgae cultivation for wastewater refinery and biomass production | |
| CN106115938B (en) | The preparation method and sewage water treatment method of magnetic bio charcoal load photosynthetic bacteria material | |
| Zhou et al. | Biosorption of zinc and copper from aqueous solutions by two freshwater green microalgae Chlorella pyrenoidosa and Scenedesmus obliquus | |
| CN108467118B (en) | Method for removing nitrogen and phosphorus in aquaculture wastewater by using immobilized algae bacteria | |
| Lu et al. | Response of microalgae Chlorella vulgaris to Cr stress and continuous Cr removal in a membrane photobioreactor | |
| Katırcıoğlu et al. | Removal of cadmium (II) ion from aqueous system by dry biomass, immobilized live and heat-inactivated Oscillatoria sp. H1 isolated from freshwater (Mogan Lake) | |
| CN108587915B (en) | Chlorella W5 capable of removing heavy metals in water with high heavy metal content and application thereof | |
| Murugesan et al. | Biosorption of cadmium by live and immobilized cells of Spirulina platensis | |
| CN108546649B (en) | Chlorella W2 capable of removing heavy metals in water with high heavy metal content and application thereof | |
| Zhang et al. | Ammonia-nitrogen and orthophosphate removal by immobilized Chlorella sp. isolated from municipal wastewater for potential use in tertiary treatment | |
| CN111621423A (en) | High-phosphorus-loading living microalgae and preparation method and application thereof | |
| CN104261570A (en) | Livestock and poultry breeding anaerobic wastewater purifying agent | |
| CN112899265A (en) | Biological activated lignite and sulfate reducing bacteria sludge immobilized particles and preparation method and application thereof | |
| CN109576159B (en) | Chlorella W4 capable of removing heavy metals in water with high heavy metal content and application thereof | |
| Zheng et al. | Biochar fungal pellet based biological immobilization reactor efficiently removed nitrate and cadmium | |
| CN108753647B (en) | Arsenic-removing immobilized pellet and application thereof | |
| CN109576160B (en) | Chlorella W3 capable of removing heavy metals in water with high heavy metal content and application thereof | |
| Katircioğlu et al. | Chromium (VI) biosorption from aqueous solutions by free and immobilized biomass of Oscillatoria sp. H1 isolated from freshwater | |
| He et al. | Preparation of different activated sludge immobilized carriers and their organic wastewater treatment performance by microbial community | |
| CN109576161B (en) | Chlorella W1 capable of removing heavy metals in water with high heavy metal content and application thereof | |
| CN110591920A (en) | Preparation method of biological adsorbent for adsorbing heavy metals | |
| Wang et al. | Experimental Study on Removal of Iron, Manganese and Copper from Water by Microalgae. | |
| CN109626582A (en) | The method for removing lead in water removal using the modified microalgae of high phosphorus culture | |
| Wilan et al. | Coppper biosorption using beads biosorbent of mixed culture microalgae | |
| CN112899168B (en) | Application of 4R-aminopentanoic acid, 4-aminopentanoic acid and/or 4-aminobutyric acid in increasing chlorophyll content in gymnocyanine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Xia Ling Inventor after: Wang Zhixin Inventor after: Song Shaoxian Inventor after: Huang Rong Inventor before: Xia Ling Inventor before: Song Shaoxian Inventor before: Wang Zhixin Inventor before: Huang Rong |
|
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200904 |