CN110591920A - Preparation method of biological adsorbent for adsorbing heavy metals - Google Patents
Preparation method of biological adsorbent for adsorbing heavy metals Download PDFInfo
- Publication number
- CN110591920A CN110591920A CN201910856249.5A CN201910856249A CN110591920A CN 110591920 A CN110591920 A CN 110591920A CN 201910856249 A CN201910856249 A CN 201910856249A CN 110591920 A CN110591920 A CN 110591920A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- heavy metals
- volume
- biosorbent
- adsorbing heavy
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Cell Biology (AREA)
- Hydrology & Water Resources (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biomedical Technology (AREA)
- Environmental & Geological Engineering (AREA)
- Medicinal Chemistry (AREA)
- Water Supply & Treatment (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a preparation method of a biological adsorbent for adsorbing heavy metals, which comprises the steps of adding a culture medium with finished volume fixing into a first conical flask, and then dropwise adding hydrochloric acid; placing the culture medium in a sterilization device for high-temperature sterilization, and cooling the sterilized culture medium; adding (NH)4)3FeC12H10O14The stock solution is added into the cooled culture medium; after the chlorella pyrenoidosa seed liquid is inoculated, placing the second conical bottle in an illumination incubator for standing culture; shaking the second conical bottle 3 times every day, culturing for 3 days, transferring the first generation algae solution to the second generation algae solution at a volume ratio of 1:1, repeating the transfer for 3 generations, activating, and adding activated proteinDiluting the chlorella pyrenoidosa algae liquid for transferring algae seeds, and performing expanded culture to obtain the heavy metal biological adsorbent. The adsorbent can remove various heavy metals in water at the same time, has high removal rate and can not cause secondary pollution to the environment.
Description
Technical Field
The invention relates to the technical field of heavy metal adsorption, in particular to a preparation method of a biological adsorbent for adsorbing heavy metals.
Background
Water resources are indispensable natural resources for human survival, but water pollution caused by economic development is increasingly serious. Heavy metals are widely used in various industries and are the most common contaminants in contaminated surface and ground water and industrial wastewater. The presence of these heavy metals in water poses a great threat to humans and other organisms. Therefore, how to treat heavy metals in wastewater has become an important issue facing current environmental protection work. The existing methods for treating heavy metal wastewater mainly comprise chemical precipitation, ion exchange, electrochemical treatment, reverse osmosis, membrane technology, distillation, electrodialysis and the like, and although the treatment methods obtain good effects to a certain extent, the treatment methods generally have the defects of incapability of simultaneously removing various metals in water, low removal rate and secondary pollution to the environment.
Disclosure of Invention
The invention aims to provide a preparation method of a biological adsorbent for adsorbing heavy metals, and aims to solve the technical problems that various metals in water cannot be simultaneously removed, the removal rate is low, and secondary pollution is caused to the environment in the prior art.
In order to achieve the above object, the present invention provides a method for preparing a biosorbent for adsorbing heavy metals, comprising the following steps:
adding NaNO into the volumetric flask3、MgSO4·7H2O、K2HPO4、Na2CO3、C6H8O7、EDTANa2、CaCl2·2H2The stock solution O and the solution A5 are subjected to constant volume by using ultrapure water to form a culture medium;
adding a culture medium with the fixed volume into the first conical flask, then dropwise adding hydrochloric acid, and sealing the opening of the first conical flask;
placing the culture medium in a sterilization device for high-temperature sterilization, and cooling the sterilized culture medium;
adding (NH)4)3FeC12H10O14Adding the stock solution into the cooled culture medium, standing, adjusting pH to 7, and refrigerating for later use;
taking a 250ml second conical bottle, inoculating the chlorella pyrenoidosa seed solution into a culture medium in a volume ratio of 10% under an aseptic condition, and placing the second conical bottle into an illumination incubator for standing culture after the chlorella pyrenoidosa is inoculated;
shaking the second conical bottle 3 times every day at regular time, transferring the first generation algae liquid into the second generation algae liquid by the inoculation amount with the volume ratio of 1:1 after culturing for 3 days, repeating the transfer for 3 generations, successfully activating, diluting the activated chlorella pyrenoidosa algae liquid, transferring the algae seeds, and performing enlarged culture to obtain the heavy metal biological adsorbent.
Wherein, NaNO is added into the volumetric flask3、MgSO4·7H2O、K2HPO4、Na2CO3、C6H8O7、EDTANa2、CaCl2·2H2The volume of the O stock solution was 10ml, and the volume of the A5 solution added was 1 ml.
Wherein, NaNO3、MgSO4·7H2O、K2HPO4、Na2CO3、C6H8O7、EDTANa2、CaCl2·2H2O、(NH4)3FeC12H10O14The stock solutions had a concentration of 1500 mg. L, respectively-1、75mg·L-1、40mg·L-1、20mg·L-1、6mg·L-1、1mg·L-1、36mg·L-1And 6 mg. L-1。
Wherein, the A5 solution consists of 286mgH3BO3、186mgMnCl2·4H2O、22mgZnSO4·7H2O、8mgCuSO4·5H2O、39mgNa2MoO4·2H2O、5mgCo(NO3)2·6H2O, dissolving with ultrapure water, and fixing the volume to 100 mL.
Wherein, after ultrapure water is added to half of the volume of the volumetric flask, 10ml of CaCl is added2·2H2And (4) O stock solution.
Wherein, three drops of hydrochloric acid are dripped, and the concentration of the dripped hydrochloric acid is 1 mol/L.
Wherein the temperature for sterilizing the culture medium is 30 deg.C, the pressure is 101KPa, and the time is 30 min.
Wherein (NH) is added to the cooled medium4)3FeC12H10O14The volume of the stock solution was 10ml, and after standing, the pH was adjusted to 7 and the degree of refrigeration was 4 ℃.
Wherein the illumination culture conditions in the illumination incubator are 22 ℃, 3000lux of illumination intensity and 12 h/12 h of light-dark ratio.
Wherein the Chlorella pyrenoidosa seed solution is selected from freshwater algae seed bank (FACHB) of the culture Collection of the Chinese academy of sciences.
The invention relates to a preparation method of a biological adsorbent for adsorbing heavy metal, which is characterized in that NaNO is added into a volumetric flask3、MgSO4·7H2O、K2HPO4、Na2CO3、C6H8O7、EDTANa2、CaCl2·2H2The stock solution O and the solution A5 are subjected to constant volume by using ultrapure water to form a culture medium; adding a culture medium with the fixed volume into the first conical flask, then dropwise adding hydrochloric acid, and sealing the opening of the first conical flask; placing the culture medium in a sterilization device for high-temperature sterilization, and cooling the sterilized culture medium; adding (NH)4)3FeC12H10O14Adding the stock solution into the cooled culture medium, standing, adjusting pH to 7, and refrigerating for later use; inoculating 250ml of Chlorella pyrenoidosa seed solution into culture medium at a volume ratio of 10% under aseptic condition, inoculating into eggPlacing the second conical bottle in an illumination incubator for standing culture after the chlorella pyrenoidosa; shaking the second conical bottle 3 times every day at regular time, transferring the first generation algae liquid into the second generation algae liquid by the inoculation amount with the volume ratio of 1:1 after culturing for 3 days, repeating the transfer for 3 generations, successfully activating, diluting the activated chlorella pyrenoidosa algae liquid, transferring the algae seeds, and performing enlarged culture to obtain the heavy metal biological adsorbent. The obtained adsorbent can remove various heavy metals in water at the same time, has high removal rate, and does not cause secondary pollution to the environment.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a flow chart of a method for preparing a biosorbent for adsorbing heavy metals according to the present invention.
FIG. 2 is an analysis chart of the removal rate of heavy metal Ni (II) by Chlorella pyrenoidosa of the present invention.
FIG. 3 is an analysis chart of the removal rate of heavy metal Cd (II) by Chlorella pyrenoidosa of the present invention.
FIG. 4 is an analysis graph showing the removal rate of heavy metal Cr (II) from Chlorella pyrenoidosa of the present invention.
FIG. 5 is an analysis graph showing the removal rate of heavy metals Hg (II) from Chlorella pyrenoidosa of the present invention.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.
In the description of the present invention, it is to be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships illustrated in the drawings, and are used merely for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, are not to be construed as limiting the present invention. Further, in the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Referring to fig. 1, the present invention provides a method for preparing a bio-adsorbent for adsorbing heavy metals, comprising the following steps:
s100: adding NaNO into the volumetric flask3、MgSO4·7H2O、K2HPO4、Na2CO3、C6H8O7、EDTANa2、CaCl2·2H2Respectively adding 10ml of O stock solution and 1ml of A5 solution, and using ultrapure water to fix the volume to 1L to form a culture medium;
in this embodiment, NaNO is added to the volumetric flask3、MgSO4·7H2O、K2HPO4、Na2CO3、C6H8O7、EDTANa2、CaCl2·2H2The volume of the O stock solution is 10mL, the volume of the A5 solution is 1mL, and the A5 solution is 286mg H3BO3、186mg MnCl2·4H2O、22mg ZnSO4·7H2O、8mg CuSO4·5H2O、39mg Na2MoO4·2H2O、5mg Co(NO3)2·6H2And O is prepared by using ultrapure water to reach the constant volume of 100 mL. NaNO3、MgSO4·7H2O、K2HPO4、Na2CO3、C6H8O7、EDTANa2、CaCl2·2H2O and the concentration of the stock solution are respectively1500mg·L-1、75mg·L-1、40mg·L-1、20mg·L-1、6mg·L-1、1mg·L-1And 36 mg. L-1. Dissolving the stock solution, and diluting to 1L to obtain culture medium. It should be noted that 10mL of CaCl was added after the ultrapure water was added to half the volume of the flask2·2H2O stock solution, which prevents Ca from being present in the initial mixing2CO3And (4) precipitating.
S200: adding the culture medium with the fixed volume into the first conical flask, then dropwise adding 3 drops of hydrochloric acid, and sealing the bottle mouth of the first conical flask by using kraft paper;
in the embodiment, the culture medium with the constant volume is added into the first conical flask, then 3 drops of hydrochloric acid are dripped by using a rubber-tipped dropper, the concentration of the selected hydrochloric acid is 1mol/L, and then the bottle mouth of the first conical flask is sealed by using kraft paper, so that impurities are prevented from entering the first conical flask and causing quality change of substances in the first conical flask.
S300: placing the culture medium in a sterilization device for high-temperature sterilization, and cooling the sterilized culture medium;
in the present embodiment, the medium in the first flask was sterilized at a high temperature by the sterilizer, and the temperature of the medium was controlled to 30 ℃, the pressure to 101KPa, and the time to 30min to sterilize the medium by the sterilizer, and after the sterilization of the medium was completed, the medium was cooled to room temperature.
S400: 10ml of (NH) are added4)3FeC12H10O14Adding the stock solution into the cooled culture medium, standing, adjusting pH to 7, and refrigerating at 4 deg.C for use;
in this embodiment, (NH) is added to the cooled medium4)3FeC12H10O14The volume of the stock solution was 10ml, and the concentration was 6 mg.L-1And after standing, adjusting the pH value to 7, and controlling the refrigeration degree to be 4 ℃, so as to prepare a culture medium suitable for the growth of the chlorella pyrenoidosa, wherein the culture medium of the chlorella pyrenoidosa is BG-11 culture medium.
S500: taking a 250ml second conical bottle, inoculating the chlorella pyrenoidosa seed solution into a culture medium in a volume ratio of 10% under an aseptic condition, and placing the second conical bottle into an illumination incubator for standing culture after the chlorella pyrenoidosa is inoculated;
in the embodiment, a second conical bottle with the volume of 250ml is selected, the chlorella pyrenoidosa seed solution is inoculated into the culture medium in a volume ratio of 10% under the aseptic condition, the second conical bottle is placed in an illumination incubator for standing culture after the chlorella pyrenoidosa is inoculated, the chlorella pyrenoidosa seed solution is selected from a freshwater algae seed bank (FACHB) of the culture Collection of the Chinese academy of sciences, and the illumination culture condition in the illumination incubator is that the temperature is controlled at 22 ℃, the illumination intensity is 3000lux, and the light-dark ratio is 12h:12 h.
S600: shaking the second conical bottle 3 times every day at regular time, transferring the first generation algae liquid into the second generation algae liquid by the inoculation amount with the volume ratio of 1:1 after culturing for 3 days, repeating the transfer for 3 generations, successfully activating, diluting the activated chlorella pyrenoidosa algae liquid, transferring the algae seeds, and performing enlarged culture to obtain the heavy metal biological adsorbent.
In the embodiment, the second conical bottle is shaken 3 times every day at regular time, and after 3 days of culture, the chlorella pyrenoidosa is subjected to repeated batch culture, namely, the first generation of chlorella solution is transferred into the second generation of chlorella solution by the inoculation amount with the volume ratio of 1:1, the second generation of chlorella solution is activated after repeated transfer for 3 generations, and the activated chlorella pyrenoidosa solution is diluted into the transferred chlorella strain to obtain the chlorella pyrenoidosa solution with the best activity and in logarithmic growth phase so as to prepare the chlorella pyrenoidosa adsorbent, namely the heavy metal biological adsorbent.
Referring to fig. 2 to 5, in order to test the adsorption effect of the prepared heavy metal bio-adsorbent, the following experiments were performed:
the prepared nickel ion concentrations are respectively as follows: 0.016, 0.031, 0.063, 0.125, 0.251, 0.502, 1.003mg/L, cadmium ion concentrations of 0.030, 0.060, 0.119, 0.238, 0.476, 0.952, 1.904mg/L, chromium ion concentrations of 0.006, 0.012, 0.024, 0.048, 0.097, 0.194, 0.387mg/L, mercury ion concentrations of 0.053, 0.107, 0.213, 0.426, 0.853, 1.705, 3.410mg/L, and 7 heavy metal mixed solutions of 5mL each.
And (3) taking 7 250mL conical flasks, respectively adding 120mL of mixed solution of the culture medium and the heavy metal and 30mL of the chlorella pyrenoidosa adsorbent, setting a blank control with only 120mL of the culture medium and 30mL of the chlorella pyrenoidosa adsorbent, and putting the flasks into an illumination incubator to start adsorption. Fully shaking the erlenmeyer flasks uniformly in an aseptic ultra-clean workbench at 12h, 24h, 48h and 72h after adsorption, taking a proper amount of algae liquid, centrifuging for 10min at 10000r/min, taking supernate, filtering the supernate with a 0.45-micron filter membrane, diluting the filtered liquid by a proper multiple, and measuring the corresponding heavy metal concentration in each erlenmeyer flask by using an inductively coupled plasma emission spectrometer, wherein the measurement result is shown in figures 2-5.
Calculation of adsorption ratio:(wherein R is the removal rate; Ci is the initial concentration of heavy metal ions; Cf is the concentration of the remaining heavy metal ions in the solution after a certain adsorption time).
As can be seen from the figures 2 to 5, the chlorella pyrenoidosa biological adsorbent has good adsorption effect on four heavy metals, the removal rate is not changed greatly along with time, and the adsorption balance can be achieved before 12h, which shows that the chlorella pyrenoidosa biological adsorbent has the capability of rapid adsorption. And the removal rate is gradually increased along with the increase of the concentration of the heavy metal, which shows that the catalyst also has adsorption potential for the heavy metal with high concentration. The removal rate of the chlorella pyrenoidosa to four heavy metals can basically reach more than 90%, the chlorella pyrenoidosa biological adsorbent has an excellent removal effect on mixed heavy metals, and the chlorella pyrenoidosa biological adsorbent is used for adsorbing heavy metals in water, so that the chlorella pyrenoidosa biological adsorbent is simple to operate, low in operation condition, wide in application range, free of specificity, low in cost, strong in repeatability and friendly to environment, and cannot cause secondary pollution to the environment.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (10)
1. A preparation method of a biological adsorbent for adsorbing heavy metals is characterized by comprising the following steps:
adding NaNO into the volumetric flask3、MgSO4·7H2O、K2HPO4、Na2CO3、C6H8O7、EDTANa2、CaCl2·2H2The stock solution O and the solution A5 are subjected to constant volume by using ultrapure water to form a culture medium;
adding a culture medium with the fixed volume into the first conical flask, then dropwise adding hydrochloric acid, and sealing the opening of the first conical flask;
placing the culture medium in a sterilization device for high-temperature sterilization, and cooling the sterilized culture medium;
adding (NH)4)3FeC12H10O14Adding the stock solution into the cooled culture medium, standing, adjusting pH to 7, and refrigerating for later use;
taking a 250ml second conical bottle, inoculating the chlorella pyrenoidosa seed solution into a culture medium in a volume ratio of 10% under an aseptic condition, and placing the second conical bottle into an illumination incubator for standing culture after the chlorella pyrenoidosa is inoculated;
shaking the second conical bottle 3 times every day at regular time, transferring the first generation algae liquid into the second generation algae liquid by the inoculation amount with the volume ratio of 1:1 after culturing for 3 days, repeating the transfer for 3 generations, successfully activating, diluting the activated chlorella pyrenoidosa algae liquid, transferring the algae seeds, and performing enlarged culture to obtain the heavy metal biological adsorbent.
2. The method of preparing a biosorbent for adsorbing heavy metals according to claim 1,
adding NaNO into the volumetric flask3、MgSO4·7H2O、K2HPO4、Na2CO3、C6H8O7、EDTANa2、CaCl2·2H2The volume of the O stock solution was 10ml, and the volume of the A5 solution added was 1 ml.
3. The method of preparing a biosorbent for adsorbing heavy metals according to claim 1,
NaNO3、MgSO4·7H2O、K2HPO4、Na2CO3、C6H8O7、EDTANa2、CaCl2·2H2O、(NH4)3FeC12H10O14the stock solutions had a concentration of 1500 mg. L, respectively-1、75mg·L-1、40mg·L-1、20mg·L-1、6mg·L-1、1mg·L-1、36mg·L-1And 6 mg. L-1。
4. The method of preparing a biosorbent for adsorbing heavy metals according to claim 1,
the A5 solution consists of 286mgH3BO3、186mgMnCl2·4H2O、22mgZnSO4·7H2O、8mgCuSO4·5H2O、39mgNa2MoO4·2H2O、5mgCo(NO3)2·6H2O, dissolving with ultrapure water, and fixing the volume to 100 mL.
5. The method of preparing a biosorbent for adsorbing heavy metals according to claim 1,
adding ultrapure water to half of the volume of the volumetric flask, and then adding 10ml of CaCl2·2H2And (4) O stock solution.
6. The method of preparing a biosorbent for adsorbing heavy metals according to claim 1,
three drops of hydrochloric acid are added dropwise, and the concentration of the dropwise added hydrochloric acid is 1 mol/L.
7. The method of preparing a biosorbent for adsorbing heavy metals according to claim 1,
the temperature for sterilizing the culture medium is 30 deg.C, the pressure is 101KPa, and the time is 30 min.
8. The method of preparing a biosorbent for adsorbing heavy metals according to claim 1,
(NH) added to the cooled culture medium4)3FeC12H10O14The volume of the stock solution was 10ml, and after standing, the pH was adjusted to 7 and the degree of refrigeration was 4 ℃.
9. The method of preparing a biosorbent for adsorbing heavy metals according to claim 1,
the illumination culture conditions in the illumination incubator are 22 ℃, 3000lux of illumination intensity and 12h:12h of light-dark ratio.
10. The method of preparing a biosorbent for adsorbing heavy metals according to claim 1,
the Chlorella pyrenoidosa seed solution is selected from freshwater algae seed bank (FACHB) of the culture Collection of the Chinese academy of sciences.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910856249.5A CN110591920A (en) | 2019-09-11 | 2019-09-11 | Preparation method of biological adsorbent for adsorbing heavy metals |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910856249.5A CN110591920A (en) | 2019-09-11 | 2019-09-11 | Preparation method of biological adsorbent for adsorbing heavy metals |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110591920A true CN110591920A (en) | 2019-12-20 |
Family
ID=68858635
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910856249.5A Pending CN110591920A (en) | 2019-09-11 | 2019-09-11 | Preparation method of biological adsorbent for adsorbing heavy metals |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110591920A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114772742A (en) * | 2022-05-19 | 2022-07-22 | 武汉轻工大学 | Method for improving selenium-containing wastewater treatment capacity of chlorella |
| CN117343843A (en) * | 2023-04-21 | 2024-01-05 | 江汉大学 | Mutant chlamydomonas reinhardtii, composite algae species, soil conditioner and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100732464B1 (en) * | 2005-12-26 | 2007-06-27 | 재단법인 포항산업과학연구원 | Heavy metal absorbent and method for removing heavy metal using the same |
| CN104148029A (en) * | 2014-08-22 | 2014-11-19 | 江苏绿田环保科技有限公司 | Preparation method and application method of heavy metal ion biological adsorbent |
| CN105044065A (en) * | 2015-08-07 | 2015-11-11 | 河北科技大学 | Preparation method for algae solution used for determining chlorophyll content through fluorescence method |
-
2019
- 2019-09-11 CN CN201910856249.5A patent/CN110591920A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100732464B1 (en) * | 2005-12-26 | 2007-06-27 | 재단법인 포항산업과학연구원 | Heavy metal absorbent and method for removing heavy metal using the same |
| CN104148029A (en) * | 2014-08-22 | 2014-11-19 | 江苏绿田环保科技有限公司 | Preparation method and application method of heavy metal ion biological adsorbent |
| CN105044065A (en) * | 2015-08-07 | 2015-11-11 | 河北科技大学 | Preparation method for algae solution used for determining chlorophyll content through fluorescence method |
Non-Patent Citations (3)
| Title |
|---|
| CHENG, PENGFEI: "Biofilm Attached Cultivation of Chlorella pyrenoidosa Is a Developed System for Swine Wastewater Treatment and Lipid Production", 《FRONTIERS IN PLANT SCIENCE》 * |
| 唐青青: "蛋白核小球藻对单一及复合污染水中重金属的生物吸附研究", 《中国优秀硕士学位论文全文数据库》 * |
| 王碧荷: "有机酸对蛋白核小球藻生物富集Pb的影响及其机理", 《万方数据知识服务平台》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114772742A (en) * | 2022-05-19 | 2022-07-22 | 武汉轻工大学 | Method for improving selenium-containing wastewater treatment capacity of chlorella |
| CN117343843A (en) * | 2023-04-21 | 2024-01-05 | 江汉大学 | Mutant chlamydomonas reinhardtii, composite algae species, soil conditioner and application thereof |
| CN117343843B (en) * | 2023-04-21 | 2024-04-19 | 江汉大学 | Mutant chlamydomonas reinhardtii, composite algae species, soil conditioner and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Deepa et al. | Sorption of Cr (VI) from dilute solutions and wastewater by live and pretreated biomass of Aspergillus flavus | |
| CN108587915B (en) | Chlorella W5 capable of removing heavy metals in water with high heavy metal content and application thereof | |
| CN108546649B (en) | Chlorella W2 capable of removing heavy metals in water with high heavy metal content and application thereof | |
| CN103834589A (en) | Achromobacter xylosoxidans and application thereof | |
| CN102423697A (en) | Immobilized biological adsorbent, its preparation method and application | |
| CN110591920A (en) | Preparation method of biological adsorbent for adsorbing heavy metals | |
| CN109576159B (en) | Chlorella W4 capable of removing heavy metals in water with high heavy metal content and application thereof | |
| CN110078220B (en) | Method and strain for in-situ remediation of arsenic-polluted high-saline water by using blue-green algae | |
| CN106591156A (en) | Epicoccum nigrum FXZ2 and application thereof | |
| CN109395700A (en) | A kind of method of cadmium in compound microalgae biological adsorption agent and its adsorbent absorption waste water | |
| CN105127195A (en) | Microorganism restoration method for soil polluted by heavy metal | |
| CN103740623B (en) | One plant height effect removes Pseudoalteromonas and the application thereof of cadmium and phosphorus in waste water | |
| CN109225163A (en) | A kind of method of cadmium in compound magnetic microalgae biological adsorption agent and its absorption waste water | |
| CN109576160B (en) | Chlorella W3 capable of removing heavy metals in water with high heavy metal content and application thereof | |
| CN109759436A (en) | The method of this category bacterium bacterial strain of the Providian of one plant of resistance to manganese and restoration of soil polluted by heavy metal | |
| CN103451105B (en) | A kind of filamentous fungus Penicllium chrysogenum J-5 of high absorption cadmium and preparation method and application | |
| CN109706096A (en) | One plant of brevibacterium frigoritolerans and its application with denitrogenation and efficient flocculating ability | |
| CN107099483B (en) | Composite biological agent and application thereof in treatment of mercury-containing wastewater | |
| Kahraman et al. | Use of various agricultural wastes for the removal of heavy metal ions | |
| CN111621423A (en) | High-phosphorus-loading living microalgae and preparation method and application thereof | |
| CN111661930A (en) | Method for repairing arsenic pollution in high-saline-alkali water body by using calcium ion enhanced blue algae-phycomycetes biomembrane | |
| CN109576161B (en) | Chlorella W1 capable of removing heavy metals in water with high heavy metal content and application thereof | |
| CN106244502A (en) | One strain efficient dephosphorization and the pseudomonas of degraded lecithin | |
| CN104129894B (en) | Cover the method that monolayer-modified biofilm zeolite in-situ continues to repair serious pollution bed mud | |
| CN112062281B (en) | Method for repairing arsenic pollution of saline-alkali water body by using blue algae-biomembrane complex |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |