CN111876332A - Preparation of algae biomembrane and application thereof in urban secondary effluent treatment - Google Patents

Preparation of algae biomembrane and application thereof in urban secondary effluent treatment Download PDF

Info

Publication number
CN111876332A
CN111876332A CN202010743202.0A CN202010743202A CN111876332A CN 111876332 A CN111876332 A CN 111876332A CN 202010743202 A CN202010743202 A CN 202010743202A CN 111876332 A CN111876332 A CN 111876332A
Authority
CN
China
Prior art keywords
microalgae
algae
biofilm
sewage
reactor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202010743202.0A
Other languages
Chinese (zh)
Inventor
张晓青
成玉
司晓光
曹军瑞
邱金泉
任华峰
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tianjin Institute of Seawater Desalination and Multipurpose Utilization MNR
Original Assignee
Tianjin Institute of Seawater Desalination and Multipurpose Utilization MNR
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tianjin Institute of Seawater Desalination and Multipurpose Utilization MNR filed Critical Tianjin Institute of Seawater Desalination and Multipurpose Utilization MNR
Priority to CN202010743202.0A priority Critical patent/CN111876332A/en
Publication of CN111876332A publication Critical patent/CN111876332A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/36—Adaptation or attenuation of cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/096—Polyesters; Polyamides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a preparation method of an algae biomembrane and application thereof in urban secondary effluent treatment, comprising the following steps: (1) performing enlarged culture on microalgae; (2) performing biofilm formation on microalgae; (3) domestication of algae membrane. The invention utilizes the algae biomembrane technology prepared from autotrophic microalgae to treat urban secondary effluent, has the advantages of simple operation, stable operation, easy culture and harvest, reusable fixed carrier and the like compared with the suspended algae technology, does not need any immobilized medicament, hardly generates negative influence on the environment, and has good application prospect in sewage treatment.

Description

Preparation of algae biomembrane and application thereof in urban secondary effluent treatment
Technical Field
The invention belongs to the technical field of water treatment, and particularly relates to a preparation method of an algae biomembrane and application thereof in urban secondary effluent treatment.
Background
At present, the municipal sewage treatment technology in China mainly comprises an activated sludge process, a biofilm process and the like, most organic pollutants in sewage can be removed by the technologies, but nutrients such as nitrogen, phosphorus and the like can only be removed by 20-40%, meanwhile, secondary effluent of municipal domestic sewage is easily influenced by factors such as influent water quality, organic carbon sources, temperature and the like, efficient nitrogen and phosphorus removal is difficult to achieve, and the effluent contains pollutants such as phosphate, ammonia nitrogen and the like with high concentration, so that water eutrophication is easily caused.
The microalgae is an easily obtained autotrophic biological resource and has the advantages of various varieties, wide distribution, strong adaptability and the like. In the growing process, the microalgae can absorb a large amount of N, P and other nutrient substances to synthesize organic matters, so that the nitrogen and phosphorus contents in the environment are reduced. A large number of studies at home and abroad prove the feasibility of microalgae for treating domestic sewage and aquaculture wastewater. The method has the advantages of low cost, high purification efficiency, no secondary pollution, no sludge treatment problem and the like in sewage treatment by utilizing the algae, can realize benign ecological cycle of the sewage, and can also reuse the generated algae biomass as feed, biofuel and the like to create economic value. However, the suspended algae cultivation system has the defects of small algae density, low treatment load, difficult harvesting of algae biomass, excessively high suspended matter, long hydraulic retention time and the like when being used for treating sewage.
The algae biofilm is formed by attaching algae on the surface of a carrier under the action of electrostatic force or the adhesion of extracellular secretions. The algae biomembrane technology is a new sewage treatment technology developed in recent years, and utilizes the characteristic that algae is adsorbed and fixed on a carrier to form high-concentration biomass, thereby improving the absorption and enrichment of pollutants such as nitrogen, phosphorus and the like in sewage. Compared with the suspension technology, the algae biofilm technology has the advantages of simple operation, stable operation, easy culture and harvest, reusable fixed carrier and the like, does not need any immobilized medicament, hardly generates negative influence on the environment, and has good application prospect in sewage treatment. A large number of researches show that the algae biofilm technology can effectively remove nutrient substances such as nitrogen, phosphorus and the like in the environment and relieve the eutrophication of the water body. Research also shows that the algae biomembrane can adapt to the environment of heavy metal ions, can effectively reduce the concentration of the heavy metal ions in the water body, and achieves the effect of purifying the water quality. The preparation of algae biofilms is a key factor affecting the application of algae biofilm technology. Besides being influenced by algae species, the nitrogen and phosphorus removal capability of the algae biofilm is directly related to the properties, surface structure, surface roughness and the like of a biofilm carrier material. In addition, it is also affected by environmental factors such as pH, temperature, and light.
Disclosure of Invention
The invention aims to overcome the defects of the suspended algae technology and provides a preparation method of a high-efficiency algae biomembrane and application thereof in urban secondary effluent denitrification and dephosphorization.
The technical scheme of the invention is as follows:
a preparation method of an algae biomembrane comprises the following steps:
(1) and (3) enlarged culture of microalgae: adding microalgae into a microalgae membrane reactor containing microalgae culture solution for expanded culture, improving the cell density of the microalgae, and expanding culture conditions: carrying out aeration culture for 5d to 8d under the conditions that the illumination intensity is 3000Lux to 4000Lux, the light-dark ratio is 12h:12h and the temperature is 23 +/-2 ℃ so as to enable the microalgae to reach the logarithmic phase;
(2) and (3) film forming of microalgae: fixing a biofilm carrier in a microalgae membrane reactor along the vertical direction, soaking the biofilm carrier in a microalgae culture solution, statically culturing for 4-7 d under the conditions that the illumination intensity is 2500Lux-4000 Lux, the light-dark ratio is 12h:12h and the temperature is 23 +/-2 ℃, and completing biofilm formation after the surface of the biofilm carrier is fully covered with algae to obtain an algae biofilm; the thickness of the prepared algae biomembrane is 50-90 μm;
(3) domestication of algae membranes: introducing sewage into the microalgae membrane reactor to acclimate the algae biomembrane in the step (2), wherein the daily update rate of the sewage in the microalgae membrane reactor is 10-30%, so that the algae biomembrane adapts to the sewage environment and is continuously replaced for 6-8 days until the sewage completely replaces the microalgae culture solution; the sewage is urban secondary effluent, and the water quality index is as follows: pH 8.0-9.0, COD 50-55 mg/L, TP 2.0-3.0 mg/L, TN 11.2-20.5 mg/L.
And the urban secondary effluent flows into the algae biofilm reactor from a water inlet of the algae biofilm reactor, and nutritional elements containing nitrogen and phosphorus in the sewage are directly absorbed and converted through the growth and propagation of microalgae on the domesticated algae biofilm.
The invention utilizes the algae biomembrane technology prepared from autotrophic microalgae to treat urban secondary effluent, has the advantages of simple operation, stable operation, easy culture and harvest, reusable fixed carrier and the like compared with the suspended algae technology, does not need any immobilized medicament, hardly generates negative influence on the environment, and has good application prospect in sewage treatment.
Drawings
FIG. 1 is a schematic diagram of a microalgae membrane reactor of the present invention;
FIG. 2-1 is a top view of the microalgae membrane reactor shown in FIG. 1;
FIG. 2-2 is a schematic diagram of the structure of a biofilm carrier in the microalgae membrane reactor shown in FIG. 2-1;
FIG. 3 is a graph showing the TN and TP removal rate of the algae biofilm prepared from chlorella in the city secondary effluent as a function of days;
FIG. 4 is a graph showing the TN and TP removal rate of the algae biofilm prepared by spirulina on the city secondary effluent as a function of the number of days;
FIG. 5 is a graph showing the TN and TP removal rate of the algae biofilm prepared from Scenedesmus obliquus in the urban secondary effluent as a function of the number of days of operation.
Detailed Description
The invention is further described below with reference to examples and figures.
The invention utilizes autotrophic microalgae resources in nature to prepare the algae biomembrane for removing nitrogen and phosphorus from the urban secondary effluent.
The preparation method of the algae biomembrane comprises the following steps:
(1) and (3) enlarged culture of microalgae: and adding the microalgae into a microalgae membrane reactor containing a microalgae culture solution for expanded culture to improve the cell density of the microalgae. Expanded culture conditions: carrying out aeration culture for 5d to 8d under the conditions that the illumination intensity is 3000Lux to 4000Lux, the light-dark ratio is 12h:12h and the temperature is 23 +/-2 ℃, so that the microalgae grow well and reach the logarithmic phase rapidly; it is preferable that the microalgae is inoculated into the culture solution in an inoculation amount of 5% by volume of the microalgae culture solution.
Preferably, the microalgae is any one of spirulina, scenedesmus, synechococcus or chlorella.
Preferably, when the microalgae is Scenedesmus obliquus, Synechococcus or Chlorella, the microalgae culture solution (culture medium) is BG-11 which is purchased from the market; when the microalgae is Spirulina, the microalgae culture solution (culture medium) is Zarrouk, and is commercially available.
The microalgae membrane reactor can be of an existing structure, and as shown in the figure, the microalgae membrane reactor usually comprises a shell made of organic glass, wherein a water inlet is formed in the front side of the left wall of the shell, the water inlet is connected with a water inlet pipeline provided with a water inlet pump, and a water outlet is formed in the rear side of the right wall of the shell.
(2) And (3) film forming of microalgae: fixing a biofilm carrier in a microalgae membrane reactor along the vertical direction, soaking the biofilm carrier in a microalgae culture solution, statically culturing for 4-7 d under the conditions that the illumination intensity is 2500Lux-4000 Lux, the light-dark ratio is 12h:12h and the temperature is 23 +/-2 ℃, and completing biofilm formation after the surface of the biofilm carrier is fully covered with algae to obtain an algae biofilm; the thickness of the prepared algae biomembrane is 50-90 μm, a large number of microalgae cells are attached to the carrier to form the algae membrane with uniform thickness, and the membrane hanging effect is good.
Preferably, the biofilm carrier is a net-shaped or rope-shaped carrier made of any one or more of polypropylene, polyethylene, polyolefin, polyamide, aldehyde cellophane, polyester fiber and natural fiber. The materials have larger comparative area and good hydrophilic performance, are beneficial to increasing the adsorption capacity of the algae cells, enable the algae cells to quickly form a membrane, and can be repeatedly utilized.
(3) Domestication of algae membranes: and (3) introducing sewage into the microalgae membrane reactor to acclimate the algae biofilm in the step (2), wherein the daily update rate (namely the volume ratio of the sewage to the solution in the reactor) of the sewage in the microalgae membrane reactor is 10-30%, so that the algae biofilm adapts to the sewage environment, and continuously replaces for 6-8 days until the sewage completely replaces the microalgae culture solution. The sewage is urban secondary effluent, and the water quality index is as follows: pH 8.0-9.0, COD 50-55 mg/L, TP 2.0-3.0 mg/L, TN 11.2-20.5 mg/L.
The invention also provides a method for treating urban secondary sewage by using the prepared algae biomembrane, which comprises the following steps: and the urban secondary effluent flows into the algae biofilm reactor from a water inlet of the algae biofilm reactor, and nutritional elements containing nitrogen and phosphorus in the sewage are directly absorbed and converted through the growth and propagation of microalgae on the domesticated algae biofilm.
Preferably, the city secondary effluent is continuously led into the algae biofilm reactor from a water inlet of the algae biofilm reactor for 1 to 2 hours, is kept stand for 6 to 8 hours, and then is continuously discharged from a water outlet of the algae biofilm reactor for 0.5 to 1 hour.
Further, the secondary effluent treatment conditions are as follows: the temperature in the algae biomembrane reactor is controlled to be 23-25 ℃, the illumination intensity is 2500-4000 Lux, the light-dark ratio is 12h:12h, and the hydraulic retention time is 4 d-8 d. The algae biomembrane can achieve the deep denitrification and dephosphorization effect on the secondary effluent, the removal rate of TP of the effluent reaches more than 90 percent after the system runs stably, and the removal rate of TN reaches more than 80 percent.
Example 1
(1) And (3) microalgae amplification culture: preparing 20L of culture solution, and adding the culture solution into the microalgae membrane reactor. The culture solution in this example was BG-11 medium, NaNO330g，K2HPO40.8g，MgSO4.7H2O 1.5g，CaCl2·7H2O 0.72g，Na2CO30.4g, citric acid 0.12g, lemon0.12g of iron and a trace element A520 mL. Transferring 1000mL of chlorella strain into BG-11 culture solution, and performing aeration culture for 6d under the conditions that the illumination intensity is 3500Lux, the light-dark ratio is 12h:12h and the temperature is 23 ℃ to ensure that the growth of the chlorella reaches logarithmic phase.
(2) Preparing the algae membrane: fixing a biological curtain of polyester fibers in a reactor, immersing the biological curtain in chlorella culture solution, statically culturing for 6d under the conditions that the illumination intensity is 3500Lux, the light-dark ratio is 12h:12h and the temperature is 23 ℃, and completing biofilm formation after the surfaces of biofilm carriers are fully covered with algae, thereby obtaining chlorella algae biofilms; the thickness of the prepared chlorella biological membrane is 70 μm.
(3) Domestication of algae membranes: domesticating the chlorella biomembrane in the step (2) according to a microorganism domestication method, wherein the sewage is urban secondary effluent, and the water quality index is as follows: pH 8.0-9.0, COD 50-55 mg/L, TP 2.0-3.0 mg/L, TN 11.2-20.5 mg/L. The daily renewal rate of the wastewater in the reactor is 20%, so that the algae biofilm adapts to the sewage environment, the continuous replacement is carried out for 7 days, and finally, the culture solution is completely replaced by the sewage.
The chlorella biological membrane prepared by the method is used for treating urban secondary sewage: and (3) introducing the urban secondary effluent into the algae biofilm reactor from a water inlet of the algae biofilm reactor, wherein the water inlet time is 1.5h, standing for 7h, and the time for discharging the treated water from a water outlet is 0.75 h. The treatment conditions are as follows: the temperature is 23 ℃, the hydraulic retention time is 5d, the illumination intensity is 3500Lux, and the light-dark ratio is 12h:12 h. The effect of chlorella biofilm on removing TP and TN from the secondary effluent is shown in figure 3, the highest removal rate of TP reaches 93.5%, and the average removal rate of TN reaches 84.1%.
Example 2
(1) And (3) microalgae amplification culture: preparing 20L of culture solution, and adding the culture solution into the microalgae membrane reactor. The culture solution of this example is Zarrouk medium NaHCO3336g，NaNO350g，K2HPO410g，MgSO4·7H2O 4.0g，NaCl 20g，K2SO420g，FeSO4·7H2O 0.2g，Na20.8g of EDTA and a trace element A520 mL. Transferring 1000mL of spirulina seeds into Zarrouk culture solution at illumination intensity of 4000Lux, light-dark ratio of 12h:12h and temperatureAnd (5) carrying out aeration culture for 5d at the temperature of 25 ℃ to ensure that the growth of the spirulina reaches logarithmic phase.
(2) Preparing the algae membrane: fixing a semi-soft carrier made of polyethylene material in a reactor, immersing the reactor in a spirulina culture solution, statically culturing for 4 days under the conditions that the illumination intensity is 4000Lux, the light-dark ratio is 12h:12h and the temperature is 25 ℃, and completing biofilm formation when the surface of a biofilm carrier is full of algae to obtain a spirulina algae biofilm; the thickness of the prepared algae biofilm is 90 μm.
(3) Domestication of algae membranes: acclimating the algae biomembrane in the step (2) according to a microorganism acclimation method, wherein the sewage is urban secondary effluent, and the water quality index is as follows: pH 8.0-9.0, COD 50-55 mg/L, TP 2.0-3.0 mg/L, TN 11.2-20.5 mg/L. The daily renewal rate of the wastewater in the reactor is 30%, so that the spirulina biological membrane adapts to the sewage environment, the continuous replacement is carried out for 6 days, and finally, the culture solution is completely replaced by the sewage.
The prepared spirulina biological membrane is used for treating urban secondary sewage: and (3) introducing the urban secondary effluent into the algae biofilm reactor from a water inlet of the algae biofilm reactor, wherein the water inlet time is 1h, standing for 6h, and the time for discharging the treated water from a water outlet is 1 h. The treatment conditions are as follows: the temperature is 25 ℃, the hydraulic retention time is 4d, the illumination intensity is 4000Lux, and the light-dark ratio is 12h:12 h. The removal effect of the spirulina biological membrane on TP and TN of the secondary effluent is shown in fig. 4, the highest removal rate of TP reaches 94.1%, and the average removal rate of TN reaches 82.9%.
Example 3
(1) And (3) microalgae amplification culture: preparing 20L of culture solution, and adding the culture solution into the microalgae membrane reactor. The culture solution in this example was BG-11 medium NaNO330g，K2HPO40.8g，MgSO4.7H2O 1.5g，CaCl2·7H2O 0.72g，Na2CO30.4gL, 0.12g of citric acid, 0.12g of ferric citrate and a trace element A520 mL. Transferring 1000mL of scenedesmus algae into BG-11 culture solution, and performing aeration culture for 8d under the conditions of illumination intensity of 3000Lux, light-dark ratio of 12h:12h and temperature of 24 ℃ to enable the scenedesmus to grow to logarithmic phase.
(2) Preparing the algae membrane: fixing a biological rope made of polypropylene in a reactor, immersing the biological rope in a scenedesmus culture solution, statically culturing for 7d under the conditions that the illumination intensity is 2500Lux, the light-dark ratio is 12h:12h and the temperature is 24 ℃, and completing biofilm formation after the surface of a biomembrane carrier is fully covered with algae to obtain a scenedesmus biomembrane; the thickness of the prepared algae biofilm is 50 μm.
(3) Domestication of algae membranes: acclimating the algae biomembrane in the step (2) according to a microorganism acclimation method, wherein the sewage is urban secondary effluent, and the water quality index is as follows: pH 8.0-9.0, COD 50-55 mg/L, TP 2.0-3.0 mg/L, TN 11.2-20.5 mg/L. The daily renewal rate of the wastewater in the reactor is 10 percent, so that the scenedesmus biological membrane adapts to the sewage environment and is continuously replaced for 8 days, and finally, the culture solution is completely replaced by the sewage.
The scenedesmus biomembrane prepared by the method is used for treating urban secondary sewage: and (3) introducing the urban secondary effluent into the algae biofilm reactor from a water inlet of the algae biofilm reactor, wherein the water inlet time is 2 hours, standing for 8 hours, and the time for discharging the treated water from a water outlet is 0.5 hour. The treatment conditions are as follows: the temperature is 24 ℃, the hydraulic retention time is 8d, the illumination intensity is 2500Lux, and the light-dark ratio is 12h:12 h. The effect of scenedesmus on removing TP and TN from the secondary effluent is shown in FIG. 5, the highest removal rate of TP reaches 91.8%, and the average removal rate of TN reaches 81.3%.

Claims (8)

1. The preparation method of the algae biomembrane is characterized by comprising the following steps:
(1) and (3) enlarged culture of microalgae: adding microalgae into a microalgae membrane reactor containing microalgae culture solution for expanded culture, improving the cell density of the microalgae, and expanding culture conditions: carrying out aeration culture for 5d to 8d under the conditions that the illumination intensity is 3000Lux to 4000Lux, the light-dark ratio is 12h:12h and the temperature is 23 +/-2 ℃ so as to enable the microalgae to reach the logarithmic phase;
(2) and (3) film forming of microalgae: fixing a biofilm carrier in a microalgae membrane reactor along the vertical direction, soaking the biofilm carrier in a microalgae culture solution, statically culturing for 4-7 d under the conditions that the illumination intensity is 2500Lux-4000 Lux, the light-dark ratio is 12h:12h and the temperature is 23 +/-2 ℃, and completing biofilm formation after the surface of the biofilm carrier is fully covered with algae to obtain an algae biofilm; the thickness of the prepared algae biomembrane is 50-90 μm;
(3) domestication of algae membranes: introducing sewage into the microalgae membrane reactor to acclimate the algae biomembrane in the step (2), wherein the daily update rate of the sewage in the microalgae membrane reactor is 10-30%, so that the algae biomembrane adapts to the sewage environment and is continuously replaced for 6-8 days until the sewage completely replaces the microalgae culture solution; the sewage is urban secondary effluent, and the water quality index is as follows: pH8.0-9.0, COD 50-55 mg/L, TP 2.0-3.0 mg/L, TN 11.2-20.5 mg/L.
2. The method of producing algal biofilm according to claim 1, wherein: inoculating the microalgae into the culture solution with an inoculation amount of 5% of the volume of the microalgae culture solution.
3. The method for preparing an algal biofilm according to claim 1 or 2, wherein: the microalgae is any one of spirulina, scenedesmus, synechococcus or chlorella.
4. The method of claim 3, wherein: when the microalgae is Scenedesmus, Synechococcus or Chlorella, the microalgae culture solution is BG-11; when the microalgae is Spirulina, the microalgae culture solution is Zarrouk.
5. The method of claim 3, wherein: the biomembrane carrier is a net-shaped or rope-shaped carrier made of any one or more of polypropylene, polyethylene, polyolefin, polyamide, aldehyde cellophane, polyester fiber and natural fiber.
6. The method for treating urban secondary sewage by using the algae biofilm prepared by the method of any one of claims 1 to 5, which is characterized by comprising the following steps: and the urban secondary effluent flows into the algae biofilm reactor from a water inlet of the algae biofilm reactor, and nutritional elements containing nitrogen and phosphorus in the sewage are directly absorbed and converted through the growth and propagation of microalgae on the domesticated algae biofilm.
7. The method for treating municipal secondary sewage by using algal biomembranes according to claim 6, wherein the step of: and continuously introducing the urban secondary effluent into the algae biofilm reactor for 1 to 2 hours from a water inlet of the algae biofilm reactor, standing for 6 to 8 hours, and continuously discharging the treated water for 0.5 to 1 hour from a water outlet of the algae biofilm reactor.
8. The method for treating municipal secondary sewage by using algal biomembranes according to claim 7, wherein the step of: and (3) secondary effluent treatment conditions: the temperature in the algae biomembrane reactor is controlled to be 23-25 ℃, the illumination intensity is 2500-4000 Lux, the light-dark ratio is 12h:12h, and the hydraulic retention time is 4 d-8 d.
CN202010743202.0A 2020-07-29 2020-07-29 Preparation of algae biomembrane and application thereof in urban secondary effluent treatment Pending CN111876332A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010743202.0A CN111876332A (en) 2020-07-29 2020-07-29 Preparation of algae biomembrane and application thereof in urban secondary effluent treatment

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010743202.0A CN111876332A (en) 2020-07-29 2020-07-29 Preparation of algae biomembrane and application thereof in urban secondary effluent treatment

Publications (1)

Publication Number Publication Date
CN111876332A true CN111876332A (en) 2020-11-03

Family

ID=73201528

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010743202.0A Pending CN111876332A (en) 2020-07-29 2020-07-29 Preparation of algae biomembrane and application thereof in urban secondary effluent treatment

Country Status (1)

Country Link
CN (1) CN111876332A (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112662564A (en) * 2021-01-18 2021-04-16 北京科技大学 Method for stripping and harvesting microalgae biofilm by using interface effect
CN112919642A (en) * 2021-03-31 2021-06-08 南开大学 Degradation device and degradation method for antibiotics in aquaculture wastewater
CN113637664A (en) * 2021-08-12 2021-11-12 南昌大学 Fiber carrier for adsorbing microalgae to form film based on straws, and preparation method and application thereof
CN114031170A (en) * 2021-11-17 2022-02-11 华中科技大学 Preparation method and application of microalgae biofilm based on modified cellulose
CN114180724A (en) * 2021-11-22 2022-03-15 江苏大学 Shallow layer algae bed reactor for deep nitrogen and phosphorus removal and working method
CN116173919A (en) * 2022-12-16 2023-05-30 天津市生态环境科学研究院(天津市环境规划院、天津市低碳发展研究中心) Carbon sink type rural domestic sewage recycling adsorption material and application

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106011122A (en) * 2016-06-28 2016-10-12 广西大学 Preparation method of algae biofilm
CN106587356A (en) * 2016-12-05 2017-04-26 新奥科技发展有限公司 Preparation method for biological membrane
CN108163972A (en) * 2018-01-19 2018-06-15 南昌大学 A kind of bacterium algae rotating biological membrane reactor system and application based on dephosphorization denitrogenation
CN110002680A (en) * 2019-04-22 2019-07-12 广西大学 With the method for algae biofilm, flat micro-filtration dual membrane system processing sewage

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106011122A (en) * 2016-06-28 2016-10-12 广西大学 Preparation method of algae biofilm
CN106587356A (en) * 2016-12-05 2017-04-26 新奥科技发展有限公司 Preparation method for biological membrane
CN108163972A (en) * 2018-01-19 2018-06-15 南昌大学 A kind of bacterium algae rotating biological membrane reactor system and application based on dephosphorization denitrogenation
CN110002680A (en) * 2019-04-22 2019-07-12 广西大学 With the method for algae biofilm, flat micro-filtration dual membrane system processing sewage

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ABDEL-RAOUF ET AL.: "Microalgae and wastewater treatment", 《SAUDI JOURNAL OF BIOLOGICAL SCIENCES》 *
马沛明等: "藻类生物膜技术脱氮除磷效果研究", 《环境科学》 *

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112662564A (en) * 2021-01-18 2021-04-16 北京科技大学 Method for stripping and harvesting microalgae biofilm by using interface effect
CN112662564B (en) * 2021-01-18 2022-04-26 北京科技大学 Method for stripping and harvesting microalgae biofilm by using interface effect
CN112919642A (en) * 2021-03-31 2021-06-08 南开大学 Degradation device and degradation method for antibiotics in aquaculture wastewater
CN112919642B (en) * 2021-03-31 2024-09-13 南开大学 Degradation device and degradation method for antibiotics in cultivation wastewater
CN113637664A (en) * 2021-08-12 2021-11-12 南昌大学 Fiber carrier for adsorbing microalgae to form film based on straws, and preparation method and application thereof
CN114031170A (en) * 2021-11-17 2022-02-11 华中科技大学 Preparation method and application of microalgae biofilm based on modified cellulose
CN114031170B (en) * 2021-11-17 2022-10-21 华中科技大学 Preparation method and application of microalgae biofilm based on modified cellulose
CN114180724A (en) * 2021-11-22 2022-03-15 江苏大学 Shallow layer algae bed reactor for deep nitrogen and phosphorus removal and working method
CN114180724B (en) * 2021-11-22 2024-04-12 江苏大学 Shallow algae bed reactor for deep denitrification and dephosphorization and working method
CN116173919A (en) * 2022-12-16 2023-05-30 天津市生态环境科学研究院(天津市环境规划院、天津市低碳发展研究中心) Carbon sink type rural domestic sewage recycling adsorption material and application
CN116173919B (en) * 2022-12-16 2024-04-12 天津市生态环境科学研究院(天津市环境规划院、天津市低碳发展研究中心) Carbon sink type rural domestic sewage recycling adsorption material and application

Similar Documents

Publication Publication Date Title
CN111876332A (en) Preparation of algae biomembrane and application thereof in urban secondary effluent treatment
Wang et al. A review of biomass immobilization in anammox and partial nitrification/anammox systems: Advances, issues, and future perspectives
CN108467118B (en) Method for removing nitrogen and phosphorus in aquaculture wastewater by using immobilized algae bacteria
CN2883355Y (en) Sewage treatment and ultilization Ecological pool
CN101560486B (en) Achromobacter xylosoxidans strain for biological denitrificaion and application thereof
CN103112951B (en) Biochemical method for treating synthetic leather wastewater containing dimethylformamide
CN102583880B (en) Treatment process of antibiotic pharmacy waste water
CN101265458A (en) Method for preparing strong film-forming bacterium and reinforcing sewage denitrogenation
Zhang et al. Ammonia-nitrogen and orthophosphate removal by immobilized Chlorella sp. isolated from municipal wastewater for potential use in tertiary treatment
WO2022217674A1 (en) Microalgae culture pond-artficial wetland coupling system and method for deep purification of sewage
CN101338292A (en) Method for preparing immobilization cold resistant strain
CN105016579A (en) Rural sewage treatment system
CN104496017B (en) A kind of method utilizing anaerobic filter to start Anammox fast
CN109081447B (en) Method for removing nitrogen and phosphorus in culture wastewater by combining chlorella, acinetobacter and pseudomonas
CN102776140A (en) Cold-tolerant pseudomonas strain Den-05, and screening method and application thereof
CN214653954U (en) Ecological floating island
Elmitwalli et al. Treatment of domestic sewage at low temperature in a two-anaerobic step system followed by a trickling filter
CN101302056A (en) Film-bioreactor processing process for sewage disposal
CN111689640A (en) Sewage treatment method
CN112358041B (en) Granular sludge culture method for synchronous denitrification and methane production and COD removal
CN115520961A (en) Diatomite reinforcement-based high ammonia nitrogen wastewater nitrosation treatment method
CN212609798U (en) MBBR suspended biological filler
CN204981491U (en) Rural sewage treatment plant
CN113233697A (en) Sewage treatment system and method based on microalgae culture
CN111689584A (en) Microbial cultivation carrier assembly

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20201103