CN102776140A - Cold-tolerant pseudomonas strain Den-05, and screening method and application thereof - Google Patents

Cold-tolerant pseudomonas strain Den-05, and screening method and application thereof Download PDF

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CN102776140A
CN102776140A CN2012101529210A CN201210152921A CN102776140A CN 102776140 A CN102776140 A CN 102776140A CN 2012101529210 A CN2012101529210 A CN 2012101529210A CN 201210152921 A CN201210152921 A CN 201210152921A CN 102776140 A CN102776140 A CN 102776140A
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aerobic
den
aerobic denitrification
bacterium
substratum
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CN102776140B (en
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马放
庞长泷
崔迪
李昂
邱天
孔祥震
蔡蕊
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HIT YIXING ACADEMY OF ENVIRONMENTAL PROTECTION
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract

A cold-tolerant pseudomonas strain Den-05 belongs to the technical field of the biological engineering and environmental engineering, and is Pseudomonas psychrophilia Den-05, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on March 27, 2012 with the preservation number of CGMCC NO.5942. Low-temperature aerobic denitrifying bacteria Pseudomonas psychrophilia Den-05 is screened from the natural environment by a microbial pure culture technique, so that the preparation of a biological denitrifying bacterial agent is realized and a high-efficiency denitrifying bacterial agent is provided for a biological enhancement system; besides, the winter denitrification effect of an urban sewage plant is improved in the control and regulation way of shortening the period of aeration by shortening the anaerobic period and the removal of nitrate nitrogen under the aerobic condition is realized; as a result, the water quality discharged is capable of meeting the requirements.

Description

The cold-resistant pseudomonas Den-05 of one strain and screening method and application
Technical field
The invention belongs to biotechnology, field of environment engineering technology.Relate to strain Pseudomonas psychrophilia Den-05 (cold-resistant pseudomonas Den-05) and screening method and the application in the municipal sewage treatment under coldcondition.
Background technology
An amount of nitrogen is the fundamental element that synthesizes mikrobes such as algae in the water body, be to form the important material conditions of aquatic ecological environment, and also be the eucyclic basis of water surrounding.But the total amount of nitrogen has surpassed the water body capacity in some water bodys of China at present, and excessive nitrogen element causes biological anomalies propagation such as algae and water, causes water quality deterioration.Simultaneously, ammonia nitrogen and nitrite nitrogen are toxic to biologies such as fish.Because how each existing way of nitrogen conversions that can circulate under certain condition controls the pollution of nitrogen in the water body in the water body, just become at present and topic that whole " 12 " reduction of discharging process all must be faced.China begun to carry out " municipal sewage plant's integrated wastewater discharge standard " (GB18918-2002) one-level A standard (ammonia nitrogen 5mg/L, TN 15mg/L), the highest priority of municipal sewage treatment has turned to the removal of organic nutrient substances such as nitrogen phosphorus.Therefore, biological denitrification method and technology have become one of water pollution control field focus to exploiting economy efficiently.
It is generally acknowledged that the denitrifying bacterium that carries out nitrate respiration all is an amphimicrobe,, just can induce nitrate reductase A and nitrite reductase that denitrification needs because only under oxygen free condition.Yet people constantly found the loss of total nitrogen under the aerobic condition in recent years; At present the aerobic denitrification phenomenon is formed reason and still do not have final conclusion; But because aerobic denitrification exists remarkable advantages than the traditional biological denitride technology, the aerobic denitrification technology becomes the new developing direction of domestic and international wastewater biological denitrificaion in recent years.The main body that aerobic denitrification is used is the aerobic denitrification flora; The aerobic denitrifying bacteria of having found at present mainly concentrates on Rhodopseudomonas (Pseudomonas), Alkaligenes (Alcaligenes), paracoccus (Paracoccus) and bacillus (Bacillus); Bacterial classification is used at most; Secondly be mechanism research, the utilization of aerobic denitrification microbial inoculum report then less.
Summary of the invention
Total N is difficult for up to standard to the WWT in winter; Long processing period, the problem that expense is high; This patent filters out the strain Pseudomonas psychrophilia Den-05 that can significantly improve biological denitrificaion efficient under the low-temperature aerobic condition from nature; And be made into biological bacteria preparation, to municipal effluent qualified discharge under the coldcondition new realization thinking is provided, to improve the Sewage Plant operational efficiency, tangible actual application value has reduced cost.
Bacterial strain provided by the invention was Pseudomonas psychrophilia Den-05, was preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", its preserving number CGMCC NO. 5942 on 03 27th, 2012.
The cold-resistant pseudomonas Den-05 of bacterial strain (Pseudomonas psychrophilia Den-05) separates to obtain from Harbin City's good faith ditch municipal wastewater treatment plant Aerobic Pond active sludge, and the domestication process of aerobic denitrification flora is all carried out under 10 ℃ of conditions.At first get in 15mL active sludge and some granulated glass spherees to the aerobic denitrification liquid nutrient medium of having sterilized shake-flask culture 4 ~ 5d.Sterilising conditions is 121 ℃, high pressure steam sterilization 20 minutes.After first culture cycle finishes, the domestication bacterium liquid that obtains joined by 15% inoculum size continue shake-flask culture 4 ~ 5d in the fresh aerobic denitrification liquid nutrient medium, repeat aforesaid operations 3 ~ 4 times.This mixed bacteria liquid is coated on the aerobic denitrification solid medium, carries out plate streaking repeatedly, solid, liquid alternated cultivation are isolated a plant height and are imitated the bacterial strain that carries out aerobic denitrification under the low temperature Pseudomonas psychrophiliaDen-05.
Concrete screening step is following:
(1) get in the fresh active sludge adding 100mL aerobic denitrification substratum triangular flask of 15mL Harbin City good faith ditch municipal effluent factory, placing temperature is 10 oC, rotating speed cultivate 4 ~ 5d in the shaking table of 150r/min, obtain enrichment bacterium liquid;
(2) get 15mL enrichment bacterium liquid and add in the 100mL aerobic denitrification substratum triangular flask, the culture condition of repeating step (1) 3 ~ 4 times obtains domestication bacterium liquid;
(3) gradient dilution domestication bacterium liquid, the dilution gradient is respectively 10 -1, 10 -2, 10 -3, 10 -4, 10 -5, 10 -6, to get 100 μ L domestication bacterium liquid respectively and add in the solid aerobic denitrification culture medium flat plate, coating method smoothens, and placing temperature is 10 ℃ incubator, cultivates 3 ~ 4d, obtains single bacterium colony;
(4) the stroke line of utilization, three rides continuation purifying obtain single bacterium colony, and aerobic denitrification substratum (liquid), solid aerobic denitrification substratum are alternately cultivated domestication, quicken to sieve the bacterium process, repeat multi-pass operations and obtain pure bacterium colony;
Wherein the composition of aerobic denitrification substratum is: 7.9 g Na 2HPO 47H 2O, 0.3 g NH 4Cl, 4.7 g Trisodium Citrates, 1.5 g KH 2PO 4, 0.1 g MgSO 47H 2O, 1.5 g KNO 3, the 1mL liquid microelement is dissolved in the 1000 mL zero(ppm) water, regulates pH 7.2; Solid aerobic denitrification substratum is added with 18g agar in addition in the aerobic denitrification substratum;
The composition of trace element is: 0.1 g CuSO 45H 2O, 0.3 g MnSO 44H 2O, 0.2 g ZnSO 47H 2O, 2.0 g NaHCO 310H 2O, 0.5 g CoCl 26H 2O, 0.05g CaCl 22H 2O, 0.5g FeSO 47H 2O, 0.02g (NH 4) 6Mo 7O 24H 2O is dissolved in the zero(ppm) water of 1000mL.
The domestication bacterium liquid OD that obtains in the step of screening method of the present invention (2) 660Be 1.0, substratum all uses behind sterilization 20 min down at 121 ℃.
Strain Pseudomonas psychrophilia Den-05 degradation capability to nitrate salt under coldcondition is extremely strong, is applied in the municipal sewage treatment under winter low temperature, the aerobic condition.
The present invention adopts the mikrobe pure culture technigne from natural habitat, to filter out low temperature, aerobic denitrifying bacteria Pseudomonas psychrophiliaDen-05; Realized the preparation of biological denitrificaion microbial inoculum; For biological fortification system provides the efficient denitrification bacterial classification, and, improve municipal wastewater treatment plant denitrification effect in winter through shortening the control methods of anaerobism cycle and then shortening aeration period; Realize the removal of nitrate nitrogen under the aerobic condition, reach the effluent quality requirement.
Description of drawings
Fig. 1 is that 108 hours thalli growth situation of aerobic denitrifying bacteria of the present invention and contaminant degradation rate change, and wherein ■ representes that the bacterium of aerobic denitrifying bacteria in 96 hours growth cycles is turbid.
Fig. 2 is the clearance trend of different nitric nitrogen concentration in the aerobic denitrifying bacteria low temperature denitrification process of the present invention; Wherein ● the Degradation and Transformation rate of expression aerobic denitrifying bacteria when nitrate salt starting point concentration 120mg/L, wherein ▲ the Degradation and Transformation rate of expression aerobic denitrifying bacteria when nitrate salt starting point concentration 60mg/L.
Embodiment
Below in conjunction with accompanying drawing the present invention is described further.
Embodiment one:
(1) get in the fresh active sludge adding 100mL aerobic denitrification substratum triangular flask of 15mL Harbin City good faith ditch municipal effluent factory, placing temperature is 10 oC, rotating speed cultivate 4 ~ 5d in the shaking table of 150r/min, obtain enrichment bacterium liquid;
(2) get 15mL enrichment bacterium liquid and add in the 100mL aerobic denitrification substratum triangular flask, the culture condition of repeating step (1) 3 ~ 4 times obtains domestication bacterium liquid, and carries out the drafting of growth curve;
(3) gradient dilution domestication bacterium liquid, the dilution gradient is respectively 10 -1, 10 -2, 10 -3, 10 -4, 10 -5, 10 -6, to get 100 μ L domestication bacterium liquid respectively and add in the solid aerobic denitrification culture medium flat plate, coating method smoothens, and placing temperature is 10 ℃ incubator, cultivates 3 ~ 4d, obtains single bacterium colony;
(4) the stroke line of utilization, three rides continuation purifying obtain single bacterium colony, and aerobic denitrification substratum (liquid), solid aerobic denitrification substratum are alternately cultivated domestication, quicken to sieve the bacterium process, repeat multi-pass operations and obtain pure bacterium colony;
Wherein the composition of aerobic denitrification substratum is: 7.9 g Na 2HPO 47H 2O, 0.3 g NH 4Cl, 4.7 g Trisodium Citrates, 1.5 g KH 2PO 4, 0.1 g MgSO 47H 2O, 1.5 g KNO 3, the 1mL liquid microelement is dissolved in the 1000 mL zero(ppm) water, regulates pH 7.2; Solid aerobic denitrification substratum is added with 18g agar in addition in the aerobic denitrification substratum;
The composition of trace element is: 0.1 g CuSO 45H 2O, 0.3 g MnSO 44H 2O, 0.2 g ZnSO 47H 2O, 2.0 g NaHCO 310H 2O, 0.5 g CoCl 26H 2O, 0.05g CaCl 22H 2O, 0.5g FeSO 47H 2O, 0.02g (NH 4) 6Mo 7O 24H 2O is dissolved in the zero(ppm) water of 1000mL.
The every 12h of domestication bacterium liquid that obtains in the step (2) gets bacterium liquid and measures OD 660, it is as shown in Figure 1 to draw growth curve behind the cultivation 120h.The bacterial strain absorbancy OD of 60h place 660Can reach peak about 1.0, after this get into decline phase.
Embodiment two: with adding in the sbr reactor device behind the strain expanded culture.Method is 10 oC, bacterium liquid is centrifugal 5 minutes under 3000 * g condition, and thalline adds in the reactor drum, and phase adding biomass is 200mLOD weekly 6601.0 the centrifugal gained thalline of bacterium liquid, add 4 cycles of thalline continuously, NO in the assaying reaction device 3 -Content.Sbr reactor device volume 12L, the cycle is 8h, ST 2h, aeration time, churning time are respectively 4h, 2h, and operation scheme is to connect behind the aeration 2h to stir 1h.Water distribution composition: contain 25.58g sodium acetate, 10g NH in every 20L water distribution 4Cl, 2g MgSO 47H 2O, 2g CaCl 22H 2O, 2g KH 2PO 4, 2g K 2HPO 4Add a certain amount of KNO 3, record continuous 20 days NO 3 -Concentration, data such as Fig. 2.
Nitric nitrogen concentration obviously raise at preceding 4 days, and this is consistent with the adding thalline time, should be residual substratum, after this NO in the thalline 3 -Concentration begins to reduce; Starting point concentration 120mg/L lowering speed is slightly slower than starting point concentration 60mg/L, but after the 7th day in two reactor drums nitric nitrogen concentration all reached about 10mg/L and steady running 13 days; Show that the aerobic denitrifying bacteria that screens has the effect that promotes that nitric nitrogen transforms; And can long-term existence in reactor drum, in the active sludge, shorten the anaerobism time and then shorten the cycle, in practical application, can realize good denitrification effect.

Claims (5)

1. the cold-resistant pseudomonas Den-05 of a strain; It is characterized in that bacterial strain is Pseudomonas psychrophilia Den-05; Be preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", its preserving number CGMCC NO. 5942 on 03 27th, 2012.
2. according to the screening method of the said low-temperature aerobic denitrifying bacterium of claim 1, it is characterized in that screening step and be:
(1) get in the fresh active sludge adding 100mL aerobic denitrification substratum triangular flask of 15mL Harbin City good faith ditch municipal effluent factory, placing temperature is 10 oC, rotating speed cultivate 4 ~ 5d in the shaking table of 150r/min, obtain enrichment bacterium liquid;
(2) get 15mL enrichment bacterium liquid and add in the 100mL aerobic denitrification substratum triangular flask, the culture condition of repeating step (1) 3 ~ 4 times obtains domestication bacterium liquid;
(3) gradient dilution domestication bacterium liquid, the dilution gradient is respectively 10 -1, 10 -2, 10 -3, 10 -4, 10 -5, 10 -6, to get 100 μ L domestication bacterium liquid respectively and add in the solid aerobic denitrification culture medium flat plate, coating method smoothens, and placing temperature is 10 ℃ incubator, cultivates 3 ~ 4d, obtains single bacterium colony;
(4) the stroke line of utilization, three rides continuation purifying obtain single bacterium colony, and aerobic denitrification substratum (liquid), solid aerobic denitrification substratum are alternately cultivated domestication, quicken to sieve the bacterium process, repeat multi-pass operations and obtain pure bacterium colony;
Wherein the composition of aerobic denitrification substratum is: 7.9 g Na 2HPO 47H 2O, 0.3 g NH 4Cl, 4.7 g Trisodium Citrates, 1.5 g KH 2PO 4, 0.1 g MgSO 47H 2O, 1.5 g KNO 3, the 1mL liquid microelement is dissolved in the 1000 mL zero(ppm) water, regulates pH 7.2; Solid aerobic denitrification substratum is added with 18g agar in addition in the aerobic denitrification substratum;
The composition of trace element is: 0.1 g CuSO 45H 2O, 0.3 g MnSO 44H 2O, 0.2 g ZnSO 47H 2O, 2.0 g NaHCO 310H 2O, 0.5 g CoCl 26H 2O, 0.05g CaCl 22H 2O, 0.5g FeSO 47H 2O, 0.02g (NH 4) 6Mo 7O 24H 2O is dissolved in the zero(ppm) water of 1000mL.
3. screening method according to claim 2 is characterized in that the domestication bacterium liquid OD that obtains in the step (2) 660Be 1.0.
4. screening method according to claim 2 is characterized in that substratum all uses behind sterilization 20 min down at 121 ℃.
5. low-temperature aerobic denitrifying bacterium according to claim 1; It is characterized in that strain Pseudomonas psychrophilia Den-05 has degradation capability to nitrate salt under coldcondition, be applied in the municipal sewage treatment under winter low temperature, the aerobic condition.
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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103232954A (en) * 2013-03-27 2013-08-07 南开大学 Aerobic denitrification bacteria and applications thereof
CN103275908A (en) * 2013-06-21 2013-09-04 黑龙江省科学院微生物研究所 Low-temperature denitrification pseudomonas fluorescens
CN113801824A (en) * 2021-10-14 2021-12-17 中交和美环境生态建设有限公司 Pseudomonas Y1 with efficient heterotrophic nitrification and aerobic denitrification function and embedded pellet and application thereof
CN114250173A (en) * 2021-12-10 2022-03-29 青岛蔚蓝赛德生物科技有限公司 Zhaodong pseudomonas strain and application thereof
CN114804373A (en) * 2022-04-30 2022-07-29 北京赛富威环境工程技术有限公司 Microbial treatment method for ultralow-temperature wastewater
CN115558625A (en) * 2022-11-10 2023-01-03 哈尔滨工业大学 Low-temperature denitrification revolve pseudomonas stutzeri strain and application thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030219865A1 (en) * 2002-01-25 2003-11-27 Council Of Scientific And Industrial Research Novel regulatory elements of cold-inducible hutU gene from the Antarctic psychrotrophic bacterium Pseudomonas Syringae
US20040045049A1 (en) * 1998-09-22 2004-03-04 James Zhang Polynucleotides and polypeptides in plants
CN101186878A (en) * 2007-12-13 2008-05-28 哈尔滨工业大学 Composite biological microbial inoculum for fast starting urban sewage plant biochemical treatment system under low-temperature
CN101200699A (en) * 2007-11-21 2008-06-18 淮海工学院 Ocean microorganism low-temperature amylase and producing strain pseudoalteromonas GS230 thereof
CN102649943A (en) * 2012-03-28 2012-08-29 哈尔滨师范大学 Bacterium for high-efficiently degrading aniline under low-temperature condition

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040045049A1 (en) * 1998-09-22 2004-03-04 James Zhang Polynucleotides and polypeptides in plants
US20030219865A1 (en) * 2002-01-25 2003-11-27 Council Of Scientific And Industrial Research Novel regulatory elements of cold-inducible hutU gene from the Antarctic psychrotrophic bacterium Pseudomonas Syringae
CN101200699A (en) * 2007-11-21 2008-06-18 淮海工学院 Ocean microorganism low-temperature amylase and producing strain pseudoalteromonas GS230 thereof
CN101186878A (en) * 2007-12-13 2008-05-28 哈尔滨工业大学 Composite biological microbial inoculum for fast starting urban sewage plant biochemical treatment system under low-temperature
CN102649943A (en) * 2012-03-28 2012-08-29 哈尔滨师范大学 Bacterium for high-efficiently degrading aniline under low-temperature condition

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
马放 等: "《一株好氧反硝化细菌生理生态特征的研究》", 《哈尔滨工业大学学报》, vol. 38, no. 4, 30 April 2006 (2006-04-30), pages 575 - 577 *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103232954A (en) * 2013-03-27 2013-08-07 南开大学 Aerobic denitrification bacteria and applications thereof
CN103275908A (en) * 2013-06-21 2013-09-04 黑龙江省科学院微生物研究所 Low-temperature denitrification pseudomonas fluorescens
CN103275908B (en) * 2013-06-21 2014-10-08 黑龙江省科学院微生物研究所 Low-temperature denitrification pseudomonas fluorescens
CN113801824A (en) * 2021-10-14 2021-12-17 中交和美环境生态建设有限公司 Pseudomonas Y1 with efficient heterotrophic nitrification and aerobic denitrification function and embedded pellet and application thereof
CN114250173A (en) * 2021-12-10 2022-03-29 青岛蔚蓝赛德生物科技有限公司 Zhaodong pseudomonas strain and application thereof
CN114250173B (en) * 2021-12-10 2022-06-14 青岛蔚蓝赛德生物科技有限公司 Zhaodong pseudomonas strain and application thereof
CN114804373A (en) * 2022-04-30 2022-07-29 北京赛富威环境工程技术有限公司 Microbial treatment method for ultralow-temperature wastewater
CN115558625A (en) * 2022-11-10 2023-01-03 哈尔滨工业大学 Low-temperature denitrification revolve pseudomonas stutzeri strain and application thereof

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