CN102776140A - Cold-tolerant pseudomonas strain Den-05, and screening method and application thereof - Google Patents
Cold-tolerant pseudomonas strain Den-05, and screening method and application thereof Download PDFInfo
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- 241000589516 Pseudomonas Species 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000012216 screening Methods 0.000 title claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 230000001580 bacterial effect Effects 0.000 claims abstract description 9
- 239000010865 sewage Substances 0.000 claims abstract description 7
- 241000894006 Bacteria Species 0.000 claims description 47
- 239000007788 liquid Substances 0.000 claims description 32
- 239000007787 solid Substances 0.000 claims description 11
- 239000000203 mixture Substances 0.000 claims description 7
- 238000010790 dilution Methods 0.000 claims description 6
- 239000012895 dilution Substances 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 6
- 239000010802 sludge Substances 0.000 claims description 6
- 230000015556 catabolic process Effects 0.000 claims description 5
- 238000006731 degradation reaction Methods 0.000 claims description 5
- 244000005700 microbiome Species 0.000 claims description 4
- 150000002823 nitrates Chemical class 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 229920001817 Agar Polymers 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 238000000576 coating method Methods 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 3
- 239000001509 sodium citrate Substances 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 235000013619 trace mineral Nutrition 0.000 claims description 3
- 239000011573 trace mineral Substances 0.000 claims description 3
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical class [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 claims description 3
- 235000019263 trisodium citrate Nutrition 0.000 claims description 3
- 238000005273 aeration Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 238000004904 shortening Methods 0.000 abstract description 4
- 238000002360 preparation method Methods 0.000 abstract description 3
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 abstract description 2
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- 230000007613 environmental effect Effects 0.000 abstract 1
- 230000000813 microbial effect Effects 0.000 abstract 1
- 238000009629 microbiological culture Methods 0.000 abstract 1
- 238000004321 preservation Methods 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 20
- 229910052757 nitrogen Inorganic materials 0.000 description 10
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 239000002609 medium Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 2
- 241001057811 Paracoccus <mealybug> Species 0.000 description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000002068 microbial inoculum Substances 0.000 description 2
- 239000010841 municipal wastewater Substances 0.000 description 2
- 238000012807 shake-flask culturing Methods 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000588986 Alcaligenes Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 108090000913 Nitrate Reductases Proteins 0.000 description 1
- 108010025915 Nitrite Reductases Proteins 0.000 description 1
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 1
- 241000190932 Rhodopseudomonas Species 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- YUWBVKYVJWNVLE-UHFFFAOYSA-N [N].[P] Chemical compound [N].[P] YUWBVKYVJWNVLE-UHFFFAOYSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 208000035844 biological anomaly Diseases 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A cold-tolerant pseudomonas strain Den-05 belongs to the technical field of the biological engineering and environmental engineering, and is Pseudomonas psychrophilia Den-05, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on March 27, 2012 with the preservation number of CGMCC NO.5942. Low-temperature aerobic denitrifying bacteria Pseudomonas psychrophilia Den-05 is screened from the natural environment by a microbial pure culture technique, so that the preparation of a biological denitrifying bacterial agent is realized and a high-efficiency denitrifying bacterial agent is provided for a biological enhancement system; besides, the winter denitrification effect of an urban sewage plant is improved in the control and regulation way of shortening the period of aeration by shortening the anaerobic period and the removal of nitrate nitrogen under the aerobic condition is realized; as a result, the water quality discharged is capable of meeting the requirements.
Description
Technical field
The invention belongs to biotechnology, field of environment engineering technology.Relate to strain Pseudomonas psychrophilia Den-05 (cold-resistant pseudomonas Den-05) and screening method and the application in the municipal sewage treatment under coldcondition.
Background technology
An amount of nitrogen is the fundamental element that synthesizes mikrobes such as algae in the water body, be to form the important material conditions of aquatic ecological environment, and also be the eucyclic basis of water surrounding.But the total amount of nitrogen has surpassed the water body capacity in some water bodys of China at present, and excessive nitrogen element causes biological anomalies propagation such as algae and water, causes water quality deterioration.Simultaneously, ammonia nitrogen and nitrite nitrogen are toxic to biologies such as fish.Because how each existing way of nitrogen conversions that can circulate under certain condition controls the pollution of nitrogen in the water body in the water body, just become at present and topic that whole " 12 " reduction of discharging process all must be faced.China begun to carry out " municipal sewage plant's integrated wastewater discharge standard " (GB18918-2002) one-level A standard (ammonia nitrogen 5mg/L, TN 15mg/L), the highest priority of municipal sewage treatment has turned to the removal of organic nutrient substances such as nitrogen phosphorus.Therefore, biological denitrification method and technology have become one of water pollution control field focus to exploiting economy efficiently.
It is generally acknowledged that the denitrifying bacterium that carries out nitrate respiration all is an amphimicrobe,, just can induce nitrate reductase A and nitrite reductase that denitrification needs because only under oxygen free condition.Yet people constantly found the loss of total nitrogen under the aerobic condition in recent years; At present the aerobic denitrification phenomenon is formed reason and still do not have final conclusion; But because aerobic denitrification exists remarkable advantages than the traditional biological denitride technology, the aerobic denitrification technology becomes the new developing direction of domestic and international wastewater biological denitrificaion in recent years.The main body that aerobic denitrification is used is the aerobic denitrification flora; The aerobic denitrifying bacteria of having found at present mainly concentrates on Rhodopseudomonas (Pseudomonas), Alkaligenes (Alcaligenes), paracoccus (Paracoccus) and bacillus (Bacillus); Bacterial classification is used at most; Secondly be mechanism research, the utilization of aerobic denitrification microbial inoculum report then less.
Summary of the invention
Total N is difficult for up to standard to the WWT in winter; Long processing period, the problem that expense is high; This patent filters out the strain Pseudomonas psychrophilia Den-05 that can significantly improve biological denitrificaion efficient under the low-temperature aerobic condition from nature; And be made into biological bacteria preparation, to municipal effluent qualified discharge under the coldcondition new realization thinking is provided, to improve the Sewage Plant operational efficiency, tangible actual application value has reduced cost.
Bacterial strain provided by the invention was Pseudomonas psychrophilia Den-05, was preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", its preserving number CGMCC NO. 5942 on 03 27th, 2012.
The cold-resistant pseudomonas Den-05 of bacterial strain (Pseudomonas psychrophilia Den-05) separates to obtain from Harbin City's good faith ditch municipal wastewater treatment plant Aerobic Pond active sludge, and the domestication process of aerobic denitrification flora is all carried out under 10 ℃ of conditions.At first get in 15mL active sludge and some granulated glass spherees to the aerobic denitrification liquid nutrient medium of having sterilized shake-flask culture 4 ~ 5d.Sterilising conditions is 121 ℃, high pressure steam sterilization 20 minutes.After first culture cycle finishes, the domestication bacterium liquid that obtains joined by 15% inoculum size continue shake-flask culture 4 ~ 5d in the fresh aerobic denitrification liquid nutrient medium, repeat aforesaid operations 3 ~ 4 times.This mixed bacteria liquid is coated on the aerobic denitrification solid medium, carries out plate streaking repeatedly, solid, liquid alternated cultivation are isolated a plant height and are imitated the bacterial strain that carries out aerobic denitrification under the low temperature
Pseudomonas psychrophiliaDen-05.
Concrete screening step is following:
(1) get in the fresh active sludge adding 100mL aerobic denitrification substratum triangular flask of 15mL Harbin City good faith ditch municipal effluent factory, placing temperature is 10
oC, rotating speed cultivate 4 ~ 5d in the shaking table of 150r/min, obtain enrichment bacterium liquid;
(2) get 15mL enrichment bacterium liquid and add in the 100mL aerobic denitrification substratum triangular flask, the culture condition of repeating step (1) 3 ~ 4 times obtains domestication bacterium liquid;
(3) gradient dilution domestication bacterium liquid, the dilution gradient is respectively 10
-1, 10
-2, 10
-3, 10
-4, 10
-5, 10
-6, to get 100 μ L domestication bacterium liquid respectively and add in the solid aerobic denitrification culture medium flat plate, coating method smoothens, and placing temperature is 10 ℃ incubator, cultivates 3 ~ 4d, obtains single bacterium colony;
(4) the stroke line of utilization, three rides continuation purifying obtain single bacterium colony, and aerobic denitrification substratum (liquid), solid aerobic denitrification substratum are alternately cultivated domestication, quicken to sieve the bacterium process, repeat multi-pass operations and obtain pure bacterium colony;
Wherein the composition of aerobic denitrification substratum is: 7.9 g Na
2HPO
47H
2O, 0.3 g NH
4Cl, 4.7 g Trisodium Citrates, 1.5 g KH
2PO
4, 0.1 g MgSO
47H
2O, 1.5 g KNO
3, the 1mL liquid microelement is dissolved in the 1000 mL zero(ppm) water, regulates pH 7.2; Solid aerobic denitrification substratum is added with 18g agar in addition in the aerobic denitrification substratum;
The composition of trace element is: 0.1 g CuSO
45H
2O, 0.3 g MnSO
44H
2O, 0.2 g ZnSO
47H
2O, 2.0 g NaHCO
310H
2O, 0.5 g CoCl
26H
2O, 0.05g CaCl
22H
2O, 0.5g FeSO
47H
2O, 0.02g (NH
4)
6Mo
7O
24H
2O is dissolved in the zero(ppm) water of 1000mL.
The domestication bacterium liquid OD that obtains in the step of screening method of the present invention (2)
660Be 1.0, substratum all uses behind sterilization 20 min down at 121 ℃.
Strain Pseudomonas psychrophilia Den-05 degradation capability to nitrate salt under coldcondition is extremely strong, is applied in the municipal sewage treatment under winter low temperature, the aerobic condition.
The present invention adopts the mikrobe pure culture technigne from natural habitat, to filter out low temperature, aerobic denitrifying bacteria
Pseudomonas psychrophiliaDen-05; Realized the preparation of biological denitrificaion microbial inoculum; For biological fortification system provides the efficient denitrification bacterial classification, and, improve municipal wastewater treatment plant denitrification effect in winter through shortening the control methods of anaerobism cycle and then shortening aeration period; Realize the removal of nitrate nitrogen under the aerobic condition, reach the effluent quality requirement.
Description of drawings
Fig. 1 is that 108 hours thalli growth situation of aerobic denitrifying bacteria of the present invention and contaminant degradation rate change, and wherein ■ representes that the bacterium of aerobic denitrifying bacteria in 96 hours growth cycles is turbid.
Fig. 2 is the clearance trend of different nitric nitrogen concentration in the aerobic denitrifying bacteria low temperature denitrification process of the present invention; Wherein ● the Degradation and Transformation rate of expression aerobic denitrifying bacteria when nitrate salt starting point concentration 120mg/L, wherein ▲ the Degradation and Transformation rate of expression aerobic denitrifying bacteria when nitrate salt starting point concentration 60mg/L.
Embodiment
Below in conjunction with accompanying drawing the present invention is described further.
Embodiment one:
(1) get in the fresh active sludge adding 100mL aerobic denitrification substratum triangular flask of 15mL Harbin City good faith ditch municipal effluent factory, placing temperature is 10
oC, rotating speed cultivate 4 ~ 5d in the shaking table of 150r/min, obtain enrichment bacterium liquid;
(2) get 15mL enrichment bacterium liquid and add in the 100mL aerobic denitrification substratum triangular flask, the culture condition of repeating step (1) 3 ~ 4 times obtains domestication bacterium liquid, and carries out the drafting of growth curve;
(3) gradient dilution domestication bacterium liquid, the dilution gradient is respectively 10
-1, 10
-2, 10
-3, 10
-4, 10
-5, 10
-6, to get 100 μ L domestication bacterium liquid respectively and add in the solid aerobic denitrification culture medium flat plate, coating method smoothens, and placing temperature is 10 ℃ incubator, cultivates 3 ~ 4d, obtains single bacterium colony;
(4) the stroke line of utilization, three rides continuation purifying obtain single bacterium colony, and aerobic denitrification substratum (liquid), solid aerobic denitrification substratum are alternately cultivated domestication, quicken to sieve the bacterium process, repeat multi-pass operations and obtain pure bacterium colony;
Wherein the composition of aerobic denitrification substratum is: 7.9 g Na
2HPO
47H
2O, 0.3 g NH
4Cl, 4.7 g Trisodium Citrates, 1.5 g KH
2PO
4, 0.1 g MgSO
47H
2O, 1.5 g KNO
3, the 1mL liquid microelement is dissolved in the 1000 mL zero(ppm) water, regulates pH 7.2; Solid aerobic denitrification substratum is added with 18g agar in addition in the aerobic denitrification substratum;
The composition of trace element is: 0.1 g CuSO
45H
2O, 0.3 g MnSO
44H
2O, 0.2 g ZnSO
47H
2O, 2.0 g NaHCO
310H
2O, 0.5 g CoCl
26H
2O, 0.05g CaCl
22H
2O, 0.5g FeSO
47H
2O, 0.02g (NH
4)
6Mo
7O
24H
2O is dissolved in the zero(ppm) water of 1000mL.
The every 12h of domestication bacterium liquid that obtains in the step (2) gets bacterium liquid and measures OD
660, it is as shown in Figure 1 to draw growth curve behind the cultivation 120h.The bacterial strain absorbancy OD of 60h place
660Can reach peak about 1.0, after this get into decline phase.
Embodiment two: with adding in the sbr reactor device behind the strain expanded culture.Method is 10
oC, bacterium liquid is centrifugal 5 minutes under 3000 * g condition, and thalline adds in the reactor drum, and phase adding biomass is 200mLOD weekly
6601.0 the centrifugal gained thalline of bacterium liquid, add 4 cycles of thalline continuously, NO in the assaying reaction device
3 -Content.Sbr reactor device volume 12L, the cycle is 8h, ST 2h, aeration time, churning time are respectively 4h, 2h, and operation scheme is to connect behind the aeration 2h to stir 1h.Water distribution composition: contain 25.58g sodium acetate, 10g NH in every 20L water distribution
4Cl, 2g MgSO
47H
2O, 2g CaCl
22H
2O, 2g KH
2PO
4, 2g K
2HPO
4Add a certain amount of KNO
3, record continuous 20 days NO
3 -Concentration, data such as Fig. 2.
Nitric nitrogen concentration obviously raise at preceding 4 days, and this is consistent with the adding thalline time, should be residual substratum, after this NO in the thalline
3 -Concentration begins to reduce; Starting point concentration 120mg/L lowering speed is slightly slower than starting point concentration 60mg/L, but after the 7th day in two reactor drums nitric nitrogen concentration all reached about 10mg/L and steady running 13 days; Show that the aerobic denitrifying bacteria that screens has the effect that promotes that nitric nitrogen transforms; And can long-term existence in reactor drum, in the active sludge, shorten the anaerobism time and then shorten the cycle, in practical application, can realize good denitrification effect.
Claims (5)
1. the cold-resistant pseudomonas Den-05 of a strain; It is characterized in that bacterial strain is Pseudomonas psychrophilia Den-05; Be preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", its preserving number CGMCC NO. 5942 on 03 27th, 2012.
2. according to the screening method of the said low-temperature aerobic denitrifying bacterium of claim 1, it is characterized in that screening step and be:
(1) get in the fresh active sludge adding 100mL aerobic denitrification substratum triangular flask of 15mL Harbin City good faith ditch municipal effluent factory, placing temperature is 10
oC, rotating speed cultivate 4 ~ 5d in the shaking table of 150r/min, obtain enrichment bacterium liquid;
(2) get 15mL enrichment bacterium liquid and add in the 100mL aerobic denitrification substratum triangular flask, the culture condition of repeating step (1) 3 ~ 4 times obtains domestication bacterium liquid;
(3) gradient dilution domestication bacterium liquid, the dilution gradient is respectively 10
-1, 10
-2, 10
-3, 10
-4, 10
-5, 10
-6, to get 100 μ L domestication bacterium liquid respectively and add in the solid aerobic denitrification culture medium flat plate, coating method smoothens, and placing temperature is 10 ℃ incubator, cultivates 3 ~ 4d, obtains single bacterium colony;
(4) the stroke line of utilization, three rides continuation purifying obtain single bacterium colony, and aerobic denitrification substratum (liquid), solid aerobic denitrification substratum are alternately cultivated domestication, quicken to sieve the bacterium process, repeat multi-pass operations and obtain pure bacterium colony;
Wherein the composition of aerobic denitrification substratum is: 7.9 g Na
2HPO
47H
2O, 0.3 g NH
4Cl, 4.7 g Trisodium Citrates, 1.5 g KH
2PO
4, 0.1 g MgSO
47H
2O, 1.5 g KNO
3, the 1mL liquid microelement is dissolved in the 1000 mL zero(ppm) water, regulates pH 7.2; Solid aerobic denitrification substratum is added with 18g agar in addition in the aerobic denitrification substratum;
The composition of trace element is: 0.1 g CuSO
45H
2O, 0.3 g MnSO
44H
2O, 0.2 g ZnSO
47H
2O, 2.0 g NaHCO
310H
2O, 0.5 g CoCl
26H
2O, 0.05g CaCl
22H
2O, 0.5g FeSO
47H
2O, 0.02g (NH
4)
6Mo
7O
24H
2O is dissolved in the zero(ppm) water of 1000mL.
3. screening method according to claim 2 is characterized in that the domestication bacterium liquid OD that obtains in the step (2)
660Be 1.0.
4. screening method according to claim 2 is characterized in that substratum all uses behind sterilization 20 min down at 121 ℃.
5. low-temperature aerobic denitrifying bacterium according to claim 1; It is characterized in that strain Pseudomonas psychrophilia Den-05 has degradation capability to nitrate salt under coldcondition, be applied in the municipal sewage treatment under winter low temperature, the aerobic condition.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103232954A (en) * | 2013-03-27 | 2013-08-07 | 南开大学 | Aerobic denitrification bacteria and applications thereof |
| CN103275908A (en) * | 2013-06-21 | 2013-09-04 | 黑龙江省科学院微生物研究所 | Low-temperature denitrification pseudomonas fluorescens |
| CN113801824A (en) * | 2021-10-14 | 2021-12-17 | 中交和美环境生态建设有限公司 | Pseudomonas Y1 with efficient heterotrophic nitrification and aerobic denitrification function and embedded pellet and application thereof |
| CN114250173A (en) * | 2021-12-10 | 2022-03-29 | 青岛蔚蓝赛德生物科技有限公司 | Zhaodong pseudomonas strain and application thereof |
| CN114804373A (en) * | 2022-04-30 | 2022-07-29 | 北京赛富威环境工程技术有限公司 | Microbial treatment method for ultralow-temperature wastewater |
| CN115558625A (en) * | 2022-11-10 | 2023-01-03 | 哈尔滨工业大学 | Low-temperature denitrification revolve pseudomonas stutzeri strain and application thereof |
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| CN103232954A (en) * | 2013-03-27 | 2013-08-07 | 南开大学 | Aerobic denitrification bacteria and applications thereof |
| CN103275908A (en) * | 2013-06-21 | 2013-09-04 | 黑龙江省科学院微生物研究所 | Low-temperature denitrification pseudomonas fluorescens |
| CN103275908B (en) * | 2013-06-21 | 2014-10-08 | 黑龙江省科学院微生物研究所 | Low-temperature denitrification pseudomonas fluorescens |
| CN113801824A (en) * | 2021-10-14 | 2021-12-17 | 中交和美环境生态建设有限公司 | Pseudomonas Y1 with efficient heterotrophic nitrification and aerobic denitrification function and embedded pellet and application thereof |
| CN114250173A (en) * | 2021-12-10 | 2022-03-29 | 青岛蔚蓝赛德生物科技有限公司 | Zhaodong pseudomonas strain and application thereof |
| CN114250173B (en) * | 2021-12-10 | 2022-06-14 | 青岛蔚蓝赛德生物科技有限公司 | Zhaodong pseudomonas strain and application thereof |
| CN114804373A (en) * | 2022-04-30 | 2022-07-29 | 北京赛富威环境工程技术有限公司 | Microbial treatment method for ultralow-temperature wastewater |
| CN115558625A (en) * | 2022-11-10 | 2023-01-03 | 哈尔滨工业大学 | Low-temperature denitrification revolve pseudomonas stutzeri strain and application thereof |
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