CN102491534B - Waste water treatment method using online bacterium throwing device - Google Patents
Waste water treatment method using online bacterium throwing device Download PDFInfo
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- CN102491534B CN102491534B CN 201110416162 CN201110416162A CN102491534B CN 102491534 B CN102491534 B CN 102491534B CN 201110416162 CN201110416162 CN 201110416162 CN 201110416162 A CN201110416162 A CN 201110416162A CN 102491534 B CN102491534 B CN 102491534B
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Abstract
The invention discloses a waste water treatment method using an online bacterium throwing device, which uses the online bacterium throwing device to apply the online culturing technique, wherein the technique includes: culturing efficient degradation bacteria online; inducing degrading and enzyme expression of the efficient degradation bacteria; and throwing the cultured efficient degradation bacteria fixed by a carrier into a waste water treatment system. The waste water treatment method is suitable for bypasses of treatment systems for organic waste water difficult in degradation. In the waste water treatment system, degradation density of effective microbes of special organic pollutant can be improved, target pollutant can be degraded rapidly and effectively, system starting is quickened, sludge property can be improved, starting time of the system is shortened, waste water treatment effect is improved, and quality of discharged water is improved. The waste water treatment method is simple in operation and low in manufacturing cost, online culturing, throwing and control of functional bacteria are realized, the problems of time consumption and labor consumption in manual bacterium throwing are solved, waste water treatment cost is reduced, and the waste water treatment method is widely applicable to the field of various waste water treatment.
Description
Technical field
The invention belongs to the environmental microbiology technical field, relate to and adopt online throwing bacterium device to handle the method for waste water, say so the more specifically online cultural method of a kind of bacterial classification and the online throwing bacterium device that is installed in the Sewage treatment systems bypass.
Background technology
Along with the development of modernization industry, harmful and noxious substance constantly increases, and high concentration hard-degraded organic waste water has certain toxic action to environmental microorganism, administers with general biological activity mud method, and start time is slower, and degradation bacteria needs the long period to tame.By in traditional biological treatment system, adding the lucky remedy such and insufficient of biological reinforcing technology of the microorganism with specific function, by the technology of the present invention online cultivation targetedly, induce with immobilization from the dominant bacteria that special role is arranged of occurring in nature screening or the high-efficiency strain that produces by genetic engineering technique, remove the target degradation product in the waste water, improve the concentration of effective microbe, enhancing is to the degradation capability of difficult degradation pollutent, improve its degradation rate, and improve original biological treatment system to the removal usefulness of target contaminant.
Summary of the invention
The present invention is the application for the biological reinforced effect that solves above-mentioned high-concentration hardly-degradable organic pollutant degradation, is used for providing the online cultivation of efficient degrading bacteria, induces, immobilization and the technology that adds.Enlarge the concentration of efficient degrading bacteria, improve degradation efficiency.This technology directly applies to used water difficult to degradate and handles.Purpose of the present invention mainly is to regulate the variation of bacterial classification at any time according to the variation of pollutant kind in the waste water and concentration, by online cultivation, induce, immobilization carries out adding of bacterial classification.Avoid by manually adding microbiobacterial agent, time and effort consuming, and increased cost of sewage disposal.
For achieving the above object, the invention discloses following technology contents:
A kind of method that adopts online throwing bacterium device to handle waste water is characterized in that by online throwings bacterium device, according to the online cultivation of bacterial classification, bacterial classification induce, microbial immobilized and online step of throwing bacterium carries out:
One, the online cultivation of bacterial classification:
(1) bacterial classification: toluene degradation bacteria pseudomonas (
Pseudomonas putida) from occurring in nature screening (document " degradation characteristic of toluene degradation bacteria and the research of biological reinforced effect ", Environmental Pollution and Control, 2011(7)); Microphenomenon of degradation of aniline Dell Ford Pseudomonas (
Delftia tsuruhatensisNCBI accession number HQ731448) screens from occurring in nature; high efficiency degradation bacterial agent is that (Foshan City's Bi Wofeng bio tech ltd provides for OBT and BZT microbiobacterial agent; be the bacterium of occurring in nature screening, approved qualified through USEPA (EPA), American National food and FAD (FDA), U.S. feed control association (AAFCO)).
(2) preparation first order seed nutrient solution: with the microorganism in above-mentioned bacterial classification or the degradation bacterial agent, in composition is LB substratum or peptone extractum carnis substratum, shake bottle activation 16-32h, to logarithmic phase (1 * 10
6Individual/ml-1 * 10
10Individual/ml), as the first order seed nutrient solution;
(3) inoculum size secondary seed nutrient solution: get the first order seed nutrient solution according to 2-10%(v/v) is connected in 500 liters the seeding tank, cultivates 16-32h, to logarithmic phase (1 * 10
6Individual/ml-1 * 10
10Individual/ml);
(4) the online cultivation of bacterial classification: the dosing mouth by the spawn culture device adds the secondary seed nutrient solution in online culture apparatus, inoculum size 2-10%(v/v); With spawn culture to logarithmic phase (1 * 10
6Individual/ml-1 * 10
10Individual/ml);
Substratum and the required medium component of (4) online cultivation that above-mentioned bacterial classification (3) secondary seed is required are: (NH
4)
2SO
41%, K
2HPO
40.3%, KH
2PO
40.7%, MgSO
40.05%, yeast extract 0.5%, peptone 0.5%, pH are 6-8.NaCl is 0.75%, micro-0.02-0.04g/L;
(5) condition control: the condition of online spawn culture is controlled by the device that online spawn culture device provides, and wherein dissolved oxygen control 1mg-5mg/L is lower than this scope agitator speed and aeration rate and improves automatically; PH is set to 6-8; Temperature control overruns at 20-30 ℃, regulates hot water and the Cold water tap of attemperator automatically.
Two, bacterial classification is induced:
Utilize vacuum primingpump to pump in the bacterial classification inducing culture device bacterial classification of online cultivation, the composition of inducing culture is: (NH
4)
2SO
41%, K
2HPO
40.3%, KH
2PO
40.7%, MgSO
40.05%, yeast extract paste 0.2%, peptone 0.2%, micro-0.02-0.04g/L adds each 100mg/L of toluene and aniline simultaneously, and in order to induce the expression of toluene and microphenomenon of degradation of aniline dioxygenase, the condition of setting is identical with online culture condition.
Three, bacterial classification immobilization:
In the immobilization device, pack into powder activated carbon or diatomite filler (0.5g/L), 1/4 of bacterial classification after inducing is pumped in the reaction tank of bacterial classification immobilization device, after soaking 4h, will induce 1/4 of back bacterial classification to add substratum once again every 4h, and divide and 4 times the bacterium liquid pump gone in the immobilization device; Through repeatedly adding the immersion of bacterium liquid and 48h, filler when saturated, is finished the immobilization of bacterial classification to the absorbed dose of bacterium.
Four, online throwing bacterium:
Online throwing bacterium of the present invention: the nutrient solution of said fixing in activated carbon or diatomite is pumped in the biological tank of activated sludge tank by the vacuum primingpump in the e Foerderanlage.Add the monitoring of bacterial classification or the cycle that adds is added to the content of supporting target contaminant in the pond by regular detection.
Implementing monitoring is the guarantee of online throwing bacterium technological system steady running.In order to guarantee sewage work's steady running, utilize the variation of microorganism species in the Modern Molecular Biotechnology DGGE monitoring mechanism monitoring reaction tank, implement the trend that monitoring adds the specific pollutant degradation bacteria that enters, in system, whether exist, and the activity of efficient degrading bacteria.And combine with the typical difficult degradation pollutent of irregular detection, determine online adding the cycle, the required efficient degrading bacteria of timely online replenishment system is the guarantee of the consistent operation of online throwing bacterium.
The invention provides the online cultural method of efficient degrading bacteria, bacterial classification induction method, bacterial classification process for fixation and online throwing bacterium device, device comprises the spawn culture device (1) that is arranged on the Sewage treatment systems bypass, bacterial classification apparatus for deivation (2), bacterial classification immobilization device (3) and e Foerderanlage (4); Spawn culture device (1) and bacterial classification apparatus for deivation (2) be connected with data analysis treatment unit (5) respectively, data processing equipment (5) is connected with printer (7) with display screen (6); Bacterial classification immobilization device (3) comprises bacterial classification immobilization attemperator, aerating apparatus and delivery port, and e Foerderanlage (4) comprises vacuum primingpump and transfer line, and delivery port is connected with the vacuum primingpump of e Foerderanlage on the bacterial classification immobilization device.Wherein data analysis treatment unit (5) comprises pH electrode, dissolved oxygen electrode, aerating apparatus, temperature electrode, attemperator, medicament throwing device and agitator, adopts intelligent PID controller to control; Temperature electrode, pH electrode and dissolved oxygen electrode are connected with display screen.The online related throwing bacterium device of technology that adds of the present invention is seen Fig. 1.
Advantage and positively effect that the present invention compared with prior art has are:
The present invention is suitable in the bypass of organic wastewater with difficult degradation thereby treatment system, in Sewage treatment systems, can improve the concentration of the effective microbe of the special organic pollutant of degraded, the target contaminant of degrading quickly and effectively, the quickening system starts, and improve the mud performance, the start time of shortening system, but the also stability of enhanced system and anti impulsion load ability simultaneously, the target contaminant of degrading quickly and effectively, under the constant situation of hydraulic detention time, can reach removal effect preferably, can reduce processing cost, improve the effect of sewage disposal, improve effluent quality.
The invention enables biological reinforced effect to become a kind of low cost, high efficiency technical process utilizes original water processing establishment, sets up online throwing bacterium device in the sewage disposal bypass.The high-efficiency strain of cultivating and adding Persistent organic pollutants is in Sewage treatment systems, and this enhancement techniques can improve water treatment scope and water treatment capacity significantly, and is easy and simple to handle, is easy to management.
Cost of manufacture of the present invention is low, has realized that the online of function yeast adds and control, and has solved the problem that manually adds the bacterial classification time and effort consuming, has reduced cost of sewage disposal, can be widely used in various field of waste water treatment.
Description of drawings
Fig. 1 is the structural representation that online spawn culture of the present invention is thrown the bacterium device;
Fig. 2 irregularly detects PCR-DGG figure;
Major parts nomenclature in the accompanying drawing:
1: spawn culture device 2: the bacterial classification apparatus for deivation
3: bacterial classification immobilization device 4: e Foerderanlage
5: data analysis treatment unit 6: display screen
7: printer.
Embodiment:
It is following that the present invention will be described in detail with reference to drawings and Examples.
Embodiment 1
Wherein toluene degradation bacteria pseudomonas (
Pseudomonas putida) from occurring in nature screening (document " degradation characteristic of toluene degradation bacteria and the research of biological reinforced effect ", Environmental Pollution and Control, 2011(7)); Microphenomenon of degradation of aniline Dell Ford Pseudomonas (
Delftia tsuruhatensisNCBI accession number HQ731448) screens from occurring in nature; high efficiency degradation bacterial agent is that (Foshan City's Bi Wofeng bio tech ltd provides for OBT and BZT microbiobacterial agent; be the bacterium of occurring in nature screening, approved qualified through USEPA (EPA), American National food and FAD (FDA), U.S. feed control association (AAFCO)).
The structure of bacterial classification apparatus for deivation 2 is identical with spawn culture device 1, adds target contaminant or analogue in bacterial classification apparatus for deivation 2, is used for inducing the expression of microbial function zymoprotein.Bacterial classification immobilization device 3 comprises bacterial classification immobilization attemperator and delivery port, is used for cultured function bacterial classification is immobilized onto carrier.E Foerderanlage 4 comprises vacuum primingpump and transfer line, is used for carrying bacterial classification to Sewage treatment systems.Delivery port is connected with the vacuum primingpump of e Foerderanlage on the bacterial classification immobilization device.Display screen 6 is the LED display curtain.
Data analysis treatment unit 5 comprises pH electrode, dissolved oxygen electrode, aerating apparatus, temperature electrode, attemperator, medicament throwing device and agitator, adopts parameters such as intelligent PID controller A.T.C, pH, mixing speed, DO.When the soda acid scope exceeded 6-8, peristaltic pump added acid, alkali; Dissolved oxygen control is lower than this scope agitator speed and aeration rate and improves automatically in the 1mg-5mg/L scope; Temperature control goes beyond the scope at 20-35 ℃, regulates hot water and the Cold water tap of attemperator automatically.
The carrier that immobilized carrier can be different according to the kind different choice of bacterial classification in the bacterial classification immobilization device 3 refers generally to floating stuffing or thermopnore carrier.
In order to control the top condition of spawn culture, the temperature electrode of data analysis treatment unit 5, pH electrode and dissolved oxygen electrode are connected with the LED display curtain, can show on the LED display curtain to throw dissolved oxygen, pH value and variation of temperature in the bacterium device.Can be online variation in the online culture apparatus of reaction microorganism, and it is implemented corresponding the rectification.
For the activity that makes toluene (aniline) degradation bacteria of passing through the high density that screening obtains of turning out improves, in bacterial classification apparatus for deivation 2, add target contaminant toluene (aniline) 100mg/L, be used for the expression of induced degradation bacterium dioxygenase, purpose is to improve the degradation efficiency of degraded.
In order to prevent that degradation bacteria from entering the loss in the Sewage treatment systems, microorganism selects activated carbon or diatomite to adsorb the bacterial classification that the efficient degradation thalline fixes efficient degradation toluene (aniline), reduce the loss of microorganism, the concentration of the effective microbe in the raising system improves degradation efficiency.
It more than is the elaboration that online spawn culture technology and online throwing bacterium apparatus system to this present invention sewage disposal bypass carry out; be used for helping to understand the present invention; but test method of the present invention is not subjected to the restriction of embodiment; the change that other people and not deviating from does under the principle of the invention, modification, substitute, combination, simplify the substitute mode that all should be equivalence, be included within protection scope of the present invention.
It is as follows to use online cultural method of the present invention to carry out the step of online spawn culture technology and online throwing bacterium:
1, online culture of strains:
(1) at first from the Ultralow Temperature Freezer of laboratory activation preserve toluene degradation bacteria pseudomonas (
Pseudomonas putida) and microphenomenon of degradation of aniline Dell Ford Pseudomonas (
Delftia tsuruhatensis);
(2) first order seed nutrient solution:
Above-mentioned pseudomonas kind and Dell Ford bacterium in being LB substratum or the cultivation of peptone extractum carnis, are shaken medium component a bottle activation 16-32h.It is 6-8 that pH is set, and temperature is 25-30 ℃, and rotating speed is that 130rpm is approximately to logarithmic phase (1 * 10
6Individual/ml-1 * 10
10Individual/ml) more than, as the first order seed nutrient solution;
(3) secondary seed nutrient solution:
Get in the first order seed nutrient solution is connected to 500 liters according to the inoculum size of 2-10% the seeding tank, pH is 6-8, cultivates 16-32h,, temperature is 25-30 ℃, dissolved oxygen is set to 3 to logarithmic phase (1 * 10
6Individual/ml-1 * 10
10Individual/ml).The seed tank culture based component is: (NH
4)
2SO
41%, K
2HPO
40.3%, KH
2PO
40.7%, MgSO
40.05%, yeast extract paste 0.5%, peptone 0.5%, NaCl are 0.75%, micro-0.02-0.04g/L;
(4) online culture of strains:
Dosing mouth by the spawn culture device adds the secondary seed nutrient solution in online culture apparatus, the inoculum size of 2-10%.The composition of online substratum is with the seed substratum.PH is 6, and dissolved oxygen control 1-5mg/L scope is lower than this scope agitator speed and aeration rate and improves automatically; Temperature control overruns at 25 ℃, regulates hot water and the Cold water tap of attemperator automatically.With spawn culture to logarithmic phase (1 * 10
6Individual/ml-1 * 10
8Individual/ml);
2, bacterial classification induced reaction:
Utilize vacuum primingpump to pump in the bacterial classification inducing culture device bacterial classification of online cultivation, the composition of inducing culture is: (NH
4)
2SO
41%, K
2HPO
40.3%, KH
2PO
40.7%, MgSO
40.05%, yeast extract paste 0.2%, peptone 0.2%, micro-0.02-0.04g/L adds toluene (aniline) 100mg/L simultaneously, and in order to the expression of induced degradation bacterium dioxygenase, the condition of setting is with online culture condition.
3, bacterial classification immobilized reactant:
Bacterial classification immobilization of the present invention: pack in the immobilization device powder activated carbon or diatomite filler (0.5-1g/L), 1/4 of bacterial classification after inducing is pumped in the reaction tank of bacterial classification immobilization device, after soaking 4h, 1/4 add substratum once every what 4h will induce the back bacterial classification again, divide and 4 times the bacterium liquid pump gone in the immobilization device.Through repeatedly adding the immersion of bacterium liquid and 48h, when filler to the absorbed dose of bacterium when saturated, finish the immobilization of bacterial classification.
4, online throwing bacterium: by the vacuum primingpump in the e Foerderanlage with said fixing in activated carbon toluene (aniline) bacterial classification according to 1%(v/v) ratio is pumped in the biological tank.After bacterial classification is added in the Aerobic Pond, regularly detect the content of target contaminant in the water, toluene concentration is initially 150mg/L in the waste water, and the aniline starting point concentration is 1g/L.Originally active sludge reduces to 24h to the time of toluene and degrading aniline by original 32h.Wastewater treatment has been carried out follow the tracks of detecting, and this technology, rises to about 87.5 % and 87 % by 70 original % and 80 % for the degradation rate of toluene and aniline.
Embodiment 3
A kind of method that adopts online throwing bacterium device to handle waste water, it is by online throwings bacterium device, according to the online cultivation of bacterial classification, bacterial classification induce, microbial immobilized and online step of throwing bacterium carries out:
One, the online cultivation of bacterial classification:
(1) bacterial classification: high efficiency degradation bacterial agent is that (Foshan City's Bi Wofeng bio tech ltd provides for OBT and BZT microbiobacterial agent, be the bacterium of occurring in nature screening, approved qualified through USEPA (EPA), American National food and FAD (FDA), U.S. feed control association (AAFCO));
(2) preparation first order seed nutrient solution: with the microorganism in above-mentioned bacterial classification or the degradation bacterial agent, in composition is LB substratum or peptone extractum carnis substratum, shake bottle activation 20h, to logarithmic phase (1 * 10
8Individual/ml), as the first order seed nutrient solution;
(3) inoculum size secondary seed nutrient solution: get the first order seed nutrient solution according to 10%(v/v) is connected in 500 liters the seeding tank, cultivates 16h, to logarithmic phase (1 * 10
8Individual/ml);
(4) the online cultivation of bacterial classification: the dosing mouth by the spawn culture device adds the secondary seed nutrient solution in online culture apparatus, inoculum size 8%(v/v); With spawn culture to logarithmic phase (1 * 10
8Individual/ml);
Substratum and the required medium component of (4) online cultivation that above-mentioned bacterial classification (3) secondary seed is required are: (NH
4)
2SO
41%, K
2HPO
40.3%, KH
2PO
40.7%, MgSO
40.05%, yeast extract 0.5%, peptone 0.5%, NaCl are 0.75%, micro-0.03g/L; PH is set to 8,
(5) condition control: the condition of online spawn culture is controlled by the device that online spawn culture device provides, and wherein dissolved oxygen control 5mg/L is lower than this scope agitator speed and aeration rate and improves automatically; PH is set to 8; Temperature control overruns at 30 ℃, regulates hot water and the Cold water tap of attemperator automatically;
Two, bacterial classification is induced:
Utilize vacuum primingpump to pump in the bacterial classification inducing culture device bacterial classification of online cultivation, the composition of inducing culture is: (NH
4)
2SO
41%, K
2HPO
40.3%, KH
2PO
40.7%, MgSO
40.05%, yeast extract paste 0.2%, peptone 0.2%, micro-0.02g/L adds each 100mg/L of toluene and aniline simultaneously, and in order to induce the expression of toluene and microphenomenon of degradation of aniline dioxygenase, the condition of setting is identical with online culture condition;
Three, bacterial classification immobilization:
The powder activated carbon (0.5g/L) of in the immobilization device, packing into, 1/4 of bacterial classification after inducing is pumped in the reaction tank of bacterial classification immobilization device, after soaking 4h, will induce 1/4 of back bacterial classification to add substratum once again every 4h, and divide and 4 times the bacterium liquid pump gone in the immobilization device; Through repeatedly adding the immersion of bacterium liquid and 48h, filler when saturated, is finished the immobilization of bacterial classification to the absorbed dose of bacterium.
Four, online throwing bacterium:
By the vacuum primingpump in the e Foerderanlage, with said fixing in the nutrient solution of diatomite according to 2 %(v/v) ratio is pumped in the easy-to-keep biological pond.Regularly detect the content of target contaminant in the water, the toluene starting point concentration is 150mg/L in the waste water, and the aniline starting point concentration is 1g/L.Originally active sludge reduces to 24h to the time of toluene and degrading aniline by original 32h.Wastewater treatment has been carried out follow the tracks of detecting, and this technology, rises to about 84 % and 92 % by 72 original % and 78 % for the degradation rate of toluene and aniline.
Embodiment 4
A kind of method that adopts online throwing bacterium device to handle waste water, it is by online throwings bacterium device, according to the online cultivation of bacterial classification, bacterial classification induce, microbial immobilized and online step of throwing bacterium carries out:
One, the online cultivation of bacterial classification:
(1) at first from the Ultralow Temperature Freezer of laboratory activation preserve toluene degradation bacteria pseudomonas (
Pseudomonas putida) and microphenomenon of degradation of aniline Dell Ford Pseudomonas (
Delftia tsuruhatensis);
(2) preparation first order seed nutrient solution: with the microorganism in above-mentioned bacterial classification or the degradation bacterial agent, in composition is LB substratum or peptone extractum carnis substratum, shake bottle activation 30h, to logarithmic phase (1 * 10
6Individual/ml-1 * 10
10Individual/ml), as the first order seed nutrient solution;
(3) inoculum size secondary seed nutrient solution: get the first order seed nutrient solution according to 5%(v/v) is connected in 500 liters the seeding tank, cultivates 16h, to logarithmic phase (1 * 10
8Individual/ml);
(4) the online cultivation of bacterial classification: the dosing mouth by the spawn culture device adds the secondary seed nutrient solution in online culture apparatus, 5 %(v/v) inoculum size; With spawn culture to logarithmic phase (1 * 10
8Individual/ml);
Substratum and the required medium component of (4) online cultivation that above-mentioned bacterial classification (3) secondary seed is required are: (NH
4)
2SO
41%, K
2HPO
40.3%, KH
2PO
40.7%, MgSO
40.05%, yeast extract 0.5%, peptone 0.5%, pH are that 6, NaCl is 0.75%, micro-0.04g/L;
(5) condition control: the condition of online spawn culture is controlled by the device that online spawn culture device provides, and wherein dissolved oxygen is controlled 4 mg/L, is lower than this scope agitator speed and aeration rate and improves automatically; PH is set to 7; Temperature control overruns at 25 ℃, regulates hot water and the Cold water tap of attemperator automatically;
Two, bacterial classification is induced:
Utilize vacuum primingpump to pump in the bacterial classification inducing culture device bacterial classification of online cultivation, the composition of inducing culture is: (NH
4)
2SO
41%, K
2HPO
40.3%, KH
2PO
40.7%, MgSO
40.05%, yeast extract paste 0.2%, peptone 0.2%, micro-0.04g/L adds each 100mg/L of toluene and aniline simultaneously, and in order to induce the expression of toluene and microphenomenon of degradation of aniline dioxygenase, the condition of setting is identical with online culture condition.
Three, bacterial classification immobilization:
The activated carbon (0.5g/L) of in the immobilization device, packing into, with 1/4 pumping in the reaction tank of bacterial classification immobilization device of inducing the back bacterial classification, soak 4h after, will induce 1/4 of back bacterial classification to add substratum once again every 4h, divide and 4 times the bacterium liquid pump gone in the immobilization device; Through repeatedly adding the immersion of bacterium liquid and 48h, filler when saturated, is finished the immobilization of bacterial classification to the absorbed dose of bacterium.
Four, online throwing bacterium:
By the vacuum primingpump in the e Foerderanlage with said fixing in activated carbon filler (aniline) bacterial classification according to 1%(v/v) ratio is pumped in the biological tank of activated sludge tank.Bacterial classification is added in the Aerobic Pond, detects the content of target contaminant in the water, and originally active sludge reduces to 24h to the time of toluene and degrading aniline by original 32h.Waste water toluene starting point concentration is 150mg/L, and the aniline starting point concentration is 1g/L.Wastewater treatment has been carried out follow the tracks of detecting, and this technology, rises to about 92.5 % and 94 % by 74 original % and 80 % for the degradation rate of toluene and aniline.
Embodiment 5
Practical application:
The online throwing of the present invention bacterium technology has been saved the cultivation domestication time of sewage work's aerobe pond active sludge about 50 days.According to above method with 1%(v/v) the toluene degradation bacteria of online cultivation and microphenomenon of degradation of aniline be added to getting well in the supporting property sludge sump of Tianjin chemical industrial park sewage work after being adsorbed on the activated carbon, carry out the scene and use.Be 150mg/L for toluene concentration in the waste water, aniline concentration is 1g/L.Wastewater treatment has been carried out following the tracks of detection, and carried out the PCR-DGGE detection.This technology, rises to about 94.5% and 98 % by original 76% and 84% for the degradation rate of toluene and aniline.Originally active sludge reduces to 24h to the time of toluene and degrading aniline by original 32h.By above experiment as can be known online throwing bacterium technology not only shorten start time, reduced retention time of sewage, and the waste water degradation efficiency is greatly enhanced.
By online add aniline and toluene efficient degrading bacteria bacterial classification after, simultaneously by irregularly detecting PCR-DGG, in Fig. 2, can see in the 1-5 hurdle two very vivid bands are arranged, illustrate that this two quasi-microorganism is higher at group's the inside content, after vivid band is approximately kept about 60 days, band deepening gradually in 6 hurdles.Illustrate that the dominant microflora advantage reduces gradually, active corresponding diminishing.Be combined with organic pollutants and measure, showing about about 60 days from the population colony assay is the interval of online throwing bacterium.
Claims (1)
1. one kind is adopted online throwing bacterium device to handle the method for waste water, it is characterized in that by online throwing bacterium device, according to the online cultivation of bacterial classification, bacterial classification induce, the step of bacterial classification immobilization and online throwing bacterium carries out:
One, the online cultivation of bacterial classification:
(1) bacterial classification: toluene degradation bacteria pseudomonas (
Pseudomonas putida); Microphenomenon of degradation of aniline Dell Ford Pseudomonas (
Delftia tsuruhatensis) or other high efficiency degradation bacterial agent; Described high efficiency degradation bacterial agent is OBT and BZT microbiobacterial agent;
(2) preparation first order seed nutrient solution: with the microorganism in above-mentioned bacterial classification or the high efficiency degradation bacterial agent, shake bottle activation 16-32h in composition is LB substratum or peptone extractum carnis substratum, to logarithmic phase, bacterial classification concentration is 1 * 10
6Individual/ml-1 * 10
10Individual/ml, as the first order seed nutrient solution;
(3) inoculum size first order seed enlarged culturing: get the first order seed nutrient solution according to 2-10%(v/v) is connected in 500 liters the seeding tank, cultivates 16-32h, and to logarithmic phase, bacterial classification concentration is 1 * 10
6Individual/ml-1 * 10
10Individual/ml;
(4) the online cultivation of bacterial classification: by the dosing mouth of spawn culture device, add the first order seed nutrient solution of enlarged culturing in online culture apparatus, inoculum size 2-10%(v/v), to logarithmic phase, bacterial classification concentration is 1 * 10 with spawn culture
6Individual/ml-1 * 10
10Individual/ml;
(5) condition control: the condition of online spawn culture is controlled by online throwing bacterium device, and wherein dissolved oxygen is controlled 1 mg/L-5mg/L, is lower than this scope agitator speed and aeration rate and improves automatically; PH is set to 6-8; Temperature control overruns at 20-30 ℃, regulates hot water and the Cold water tap of attemperator automatically;
The medium component of first order seed enlarged culturing (3) and the online cultivation of bacterial classification (4) is: (NH
4)
2SO
41%, K
2HPO
40.3%, KH
2PO
40.7%, MgSO
40.05%, yeast extract 0.5%, peptone 0.5%, pH are 6-8, NaCl is 0.75%, wherein micro-0.02-0.04g/L;
Two, bacterial classification is induced:
Utilize vacuum primingpump to pump in the bacterial classification apparatus for deivation bacterial classification of online cultivation, obtain to induce bacteria culture fluid; The composition of inducing culture is: (NH
4)
2SO
41%, K
2HPO
40.3%, KH
2PO
40.7%, MgSO
40.05%, yeast extract paste 0.2%, peptone 0.2%, micro-0.02-0.04g/L adds each 100mg/L of toluene and aniline simultaneously, and in order to induce the expression of toluene and microphenomenon of degradation of aniline dioxygenase, the condition of setting is identical with online culture condition;
Three, bacterial classification immobilization:
In bacterial classification immobilization device, pack into powder activated carbon or diatomite filler, one liter of inducing culture liquid of every adding, add 0.5g powder activated carbon or diatomite filler, the bacterium liquid of inducing culture in the bacterial classification apparatus for deivation divided pump into for 4 times in the bacterial classification immobilization device, whenever pump into once, soak 4h, then pump into next time again, all be pumped into the bacterium liquid of inducing in the apparatus for deivation through 4 times, after soaking 48h, filler when saturated, is finished the immobilization of bacterial classification to the absorbed dose of the bacterium in the inducing culture bacterium liquid;
Four, online throwing bacterium:
By the vacuum primingpump in the e Foerderanlage, the nutrient solution of said fixing on biologic packing material is pumped in the aerobe pond; Be added to the content of target contaminant in the aerobe pond and the variation of the efficient degradation bacterial content that utilizes molecular biology DGGE-PCR to monitor to add determines to add the gap periods of bacterial classification by regular detection;
Wherein said online throwing bacterium device comprises: online culture apparatus (1), bacterial classification apparatus for deivation (2), bacterial classification immobilization device (3) and e Foerderanlage (4); Online culture apparatus (1) is connected with data analysis treatment unit (5) respectively with bacterial classification apparatus for deivation (2), and data analysis treatment unit (5) is connected with printer (7) with LED display curtain (6); Bacterial classification immobilization device (3) comprises bacterial classification immobilization attemperator, aerating apparatus and delivery port; E Foerderanlage (4) comprises vacuum primingpump and transfer line; Bacterial classification immobilization device (3) and delivery port are connected with vacuum primingpump on the e Foerderanlage (4).
Priority Applications (1)
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|---|---|---|---|
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| CN106635918B (en) * | 2017-01-16 | 2020-04-17 | 轻工业环境保护研究所 | Delftia sp and application thereof in excess sludge reduction and dehydration |
| CN109020094B (en) * | 2018-09-26 | 2019-07-16 | 乐昌市龙辉环保科技有限公司 | A kind of biotechnology sewage-treatment plant |
| CN113562840B (en) * | 2021-06-09 | 2022-09-02 | 广州奥桑味精食品有限公司 | Method for treating monosodium glutamate wastewater based on full aerobic process |
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| CN1384065A (en) * | 2001-04-28 | 2002-12-11 | 沈阳碧水蓝天环境科技有限公司 | Biological treating process of high-concentration organic waste water |
| JP4317964B2 (en) * | 2002-02-06 | 2009-08-19 | 株式会社マサキ・エンヴェック | Wastewater treatment system using humic substances |
| CN101768564A (en) * | 2010-02-09 | 2010-07-07 | 北京航空航天大学 | Preparation of efficient phenol-degrading halophilic bacteria and application thereof |
| CN202347005U (en) * | 2011-12-14 | 2012-07-25 | 天津市环境保护科学研究院 | Bacteria feeding device for online strain culture |
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| CN1384065A (en) * | 2001-04-28 | 2002-12-11 | 沈阳碧水蓝天环境科技有限公司 | Biological treating process of high-concentration organic waste water |
| JP4317964B2 (en) * | 2002-02-06 | 2009-08-19 | 株式会社マサキ・エンヴェック | Wastewater treatment system using humic substances |
| CN101768564A (en) * | 2010-02-09 | 2010-07-07 | 北京航空航天大学 | Preparation of efficient phenol-degrading halophilic bacteria and application thereof |
| CN202347005U (en) * | 2011-12-14 | 2012-07-25 | 天津市环境保护科学研究院 | Bacteria feeding device for online strain culture |
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| 甲苯降解菌的降解特性及生物强化作用的研究;段云霞等;《环境污染与防治》;20110731;第33卷(第7期);第50-53页 * |
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