CN109678257A - A method of being suitable for the functional flora construction method and application flora improvement black and odorous water of black and odorous water improvement - Google Patents
A method of being suitable for the functional flora construction method and application flora improvement black and odorous water of black and odorous water improvement Download PDFInfo
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- CN109678257A CN109678257A CN201910156516.8A CN201910156516A CN109678257A CN 109678257 A CN109678257 A CN 109678257A CN 201910156516 A CN201910156516 A CN 201910156516A CN 109678257 A CN109678257 A CN 109678257A
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 168
- 238000010276 construction Methods 0.000 title claims abstract description 24
- CVTZKFWZDBJAHE-UHFFFAOYSA-N [N].N Chemical compound [N].N CVTZKFWZDBJAHE-UHFFFAOYSA-N 0.000 claims abstract description 150
- 235000015097 nutrients Nutrition 0.000 claims abstract description 57
- 244000005700 microbiome Species 0.000 claims abstract description 38
- 238000000034 method Methods 0.000 claims abstract description 32
- 238000011081 inoculation Methods 0.000 claims abstract description 13
- 239000002352 surface water Substances 0.000 claims abstract description 8
- 239000010802 sludge Substances 0.000 claims description 47
- -1 nitrite nitrogen Chemical compound 0.000 claims description 41
- 150000003863 ammonium salts Chemical class 0.000 claims description 24
- 238000001514 detection method Methods 0.000 claims description 23
- 239000000203 mixture Substances 0.000 claims description 23
- 241000894006 Bacteria Species 0.000 claims description 22
- UIIMBOGNXHQVGW-UHFFFAOYSA-M sodium bicarbonate Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 19
- 238000005273 aeration Methods 0.000 claims description 16
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 16
- 150000003839 salts Chemical class 0.000 claims description 13
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 13
- 239000011780 sodium chloride Substances 0.000 claims description 13
- 239000000969 carrier Substances 0.000 claims description 11
- 238000005276 aerator Methods 0.000 claims description 10
- 229910052757 nitrogen Inorganic materials 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 9
- 230000000050 nutritive Effects 0.000 claims description 9
- 239000000945 filler Substances 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 7
- 239000010865 sewage Substances 0.000 claims description 7
- 239000010457 zeolite Substances 0.000 claims description 7
- VTYYLEPIZMXCLO-UHFFFAOYSA-L calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 6
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 6
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- 239000008399 tap water Substances 0.000 claims description 6
- 235000020679 tap water Nutrition 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- 229920000742 Cotton Polymers 0.000 claims description 4
- 229920002678 cellulose Polymers 0.000 claims description 4
- 239000001913 cellulose Substances 0.000 claims description 4
- 235000016709 nutrition Nutrition 0.000 claims description 4
- 230000035764 nutrition Effects 0.000 claims description 4
- 230000001502 supplementation Effects 0.000 claims description 4
- 229960003563 Calcium Carbonate Drugs 0.000 claims description 3
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 3
- 230000000875 corresponding Effects 0.000 claims description 3
- 230000000737 periodic Effects 0.000 claims description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 27
- 239000003643 water by type Substances 0.000 abstract description 4
- 230000002045 lasting Effects 0.000 abstract description 2
- 238000005728 strengthening Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 81
- 238000005516 engineering process Methods 0.000 description 13
- 230000015556 catabolic process Effects 0.000 description 11
- 230000004059 degradation Effects 0.000 description 11
- 238000006731 degradation reaction Methods 0.000 description 11
- CDBYLPFSWZWCQE-UHFFFAOYSA-L sodium carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 10
- 239000002068 microbial inoculum Substances 0.000 description 8
- 230000001546 nitrifying Effects 0.000 description 8
- 241000276438 Gadus morhua Species 0.000 description 7
- 238000004364 calculation method Methods 0.000 description 7
- 235000019516 cod Nutrition 0.000 description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 6
- 229910052799 carbon Inorganic materials 0.000 description 6
- 235000017557 sodium bicarbonate Nutrition 0.000 description 6
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonium chloride Substances [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 5
- BFNBIHQBYMNNAN-UHFFFAOYSA-N Ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 5
- 239000001099 ammonium carbonate Substances 0.000 description 5
- 235000019270 ammonium chloride Nutrition 0.000 description 5
- VHUUQVKOLVNVRT-UHFFFAOYSA-N ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 5
- 235000011130 ammonium sulphate Nutrition 0.000 description 5
- 230000001965 increased Effects 0.000 description 5
- QDHHCQZDFGDHMP-UHFFFAOYSA-N monochloramine Chemical compound ClN QDHHCQZDFGDHMP-UHFFFAOYSA-N 0.000 description 5
- 235000011121 sodium hydroxide Nutrition 0.000 description 5
- 229910000013 Ammonium bicarbonate Inorganic materials 0.000 description 4
- 241000108664 Nitrobacteria Species 0.000 description 4
- 235000012538 ammonium bicarbonate Nutrition 0.000 description 4
- ATRRKUHOCOJYRX-UHFFFAOYSA-N azanium;hydron;carbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 description 4
- 239000003344 environmental pollutant Substances 0.000 description 4
- 239000004615 ingredient Substances 0.000 description 4
- 231100000719 pollutant Toxicity 0.000 description 4
- 239000001187 sodium carbonate Substances 0.000 description 4
- 229910000029 sodium carbonate Inorganic materials 0.000 description 4
- 239000007788 liquid Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 230000003014 reinforcing Effects 0.000 description 3
- 230000004083 survival Effects 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 244000005706 microflora Species 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 230000035943 smell Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 230000001954 sterilising Effects 0.000 description 2
- 238000005429 turbidity Methods 0.000 description 2
- 239000004912 1,5-cyclooctadiene Substances 0.000 description 1
- PRKQVKDSMLBJBJ-UHFFFAOYSA-N Ammonium carbonate Chemical compound N.N.OC(O)=O PRKQVKDSMLBJBJ-UHFFFAOYSA-N 0.000 description 1
- 241000194107 Bacillus megaterium Species 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 229910020936 NaC Inorganic materials 0.000 description 1
- FGIUAXJPYTZDNR-UHFFFAOYSA-N Potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 1
- 235000015450 Tilia cordata Nutrition 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 241001148470 aerobic bacillus Species 0.000 description 1
- 235000012501 ammonium carbonate Nutrition 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 244000052616 bacterial pathogens Species 0.000 description 1
- 230000001488 breeding Effects 0.000 description 1
- FYHXNYLLNIKZMR-UHFFFAOYSA-N calcium;carbonic acid Chemical compound [Ca].OC(O)=O FYHXNYLLNIKZMR-UHFFFAOYSA-N 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000003247 decreasing Effects 0.000 description 1
- 230000000593 degrading Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000002708 enhancing Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 125000004435 hydrogen atoms Chemical class [H]* 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000000813 microbial Effects 0.000 description 1
- 230000002906 microbiologic Effects 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006011 modification reaction Methods 0.000 description 1
- 230000001932 seasonal Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/007—Contaminated open waterways, rivers, lakes or ponds
Abstract
The present invention provides a kind of method of functional flora construction method for being suitable for black and odorous water improvement and application flora improvement black and odorous water, functional flora construction method mainly includes the following steps: that (1) indigenous microorganism samples；(2) it constructs the functional flora under black smelly water environment: preparing nutrient solution；Inoculation；Level-one culture；Second level culture.Mainly include the following steps: that (1) adds operation using the method that the flora administers black and odorous water；(2) it periodically adds and persistently cultivates.The present invention administers the live functional flora for establishing complete set in black and odorous water and " continuously cultivate-periodically adds " integrated process, the lasting ammonia nitrogen removal effect for strengthening administered water body.The present invention has broad applicability to all kinds of black-odor riverways, black smelly lake, the black smelly surface water of contaminated site etc., the building of functional flora does not depart from former aquatic environment, and flora forms dynamic change, adaptable, significant effect is particularly suitable for administering field conduct in various black and odorous waters.
Description
Technical field
The invention belongs to biochemical environmental technology fields, provide a kind of functional flora building for being suitable for black and odorous water improvement
The method of method and application flora improvement black and odorous water, this method may be implemented to administer in black and odorous water live micro- using original inhabitants
The functional flora that biology and seed sludge establish complete set " continuously cultivate-periodically adds " integrated process, persistently strengthens institute
Administer the ammonia nitrogen removal effect of water body.
Background technique
With the fast development of China's urban economy, city size increasingly expands, and urban environment infrastructure is increasingly insufficient,
Urban wastewater discharge is continuously increased, and a large amount of pollutants enter river, lake, and the pollutant concentrations such as COD in water body, nitrogen, phosphorus are super
Mark, water body occur seasonal or black throughout the year smelly.The ecosystem structure serious unbalance of city black and odorous water, influences landscape and the mankind
Production and health become current Urban Water Environment problem extremely outstanding.
At present common black and odorous water Treatment process include shut off, dredging, ecological management etc., wherein microbiological treatment skill
Art is obvious for removing the pollutants effects such as COD, nitrogen, phosphorus in water body, has good development prospect.
Ammonia nitrogen is one of important feature pollutant of black and odorous water, ammonia nitrogen index of the China for different brackets black and odorous water
It proposes and clearly requires.Microbial method removal ammonia nitrogen is increasingly becoming the research hotspot in black smelly water harnessing field.Such as China is specially
Sharp CN107699513 discloses a kind of dedicated degradation bacteria of black and odorous water and its application, mainly utilizes one plant of bacillus megaterium
Ammonia nitrogen and COD in degradation black and odorous water.Chinese patent CN106865754 discloses a kind of black smelly water of inoculation of activated-sludge progress
Body administers the method and apparatus with maintenance, is added directly using sewage plant secondary settling tank activated sludge as microorganism bacterium source.China
Patent CN107619810 discloses mixed bacteria, strain carrier and the side of ammonia nitrogen total nitrogen in sustainable degradation creek black and odorous water
Law technology administers black and odorous water using a variety of commercially available microbial inoculums.
But above-mentioned technology and product all have certain limitation, and the environmental suitability of single culture is difficult to ensure, it is difficult to advantage
Growth or efficient degradation ammonia nitrogen；Its research and development of activated sludge and commercially available microbial inoculum without special domestication and production process lose contact with reality
Black smelly water environment, product is not targeted to specific body of water, and the adaptability for treating improvement black and odorous water is difficult to ensure, therefore its
The scope of application and validity of scale application are restricted, and it is possible to because of causing and black smelly water due to adding for external source strain
The overlapping of body original inhabitants' culture ecological position, causes adverse effect to system.Simultaneously as nitrobacteria is more sensitive to environmental change,
It is easy to be impacted in black smelly water environment, therefore needs to continue in many cases largely to add, and commercially available microbial inoculum product is most
Expensive, use cost is high.From the point of view of existing research achievement, scene is administered in black and odorous water, by utilizing the micro- life of original inhabitants
The functional flora that object and seed sludge establish complete set " continuously cultivate-periodically adds " integrated process, to continue to strengthen institute
Technical method and the case history for administering the ammonia nitrogen removal effect of water body are still rare.
Summary of the invention
The present invention is administered applied to black and odorous water In view of the above shortcomings of the prior art, by biological reinforcing technology, is mentioned
A kind of functional flora construction method for being suitable for black and odorous water improvement and the method for administering black and odorous water using the flora have been supplied,
Administer the functional flora " continuously cultivate-periodically adding " of complete set is established at scene using indigenous microorganism and seed sludge one
Fluidized flow journey realizes the lasting reinforcing of ammonia nitrogen removal effect.The method of the invention is to all kinds of black-odor riverways, black smelly lake, dirt
Contaminating black smelly surface water in place etc. has broad applicability, overcomes deficiency existing for the said goods and technology, the structure of functional flora
It builds and does not depart from former black and odorous water aquatic environment, condition of culture is set using practical water quality characteristics as foundation, and flora composition dynamic becomes
Change, adaptable, significant effect will not cause adverse effect to processing system because of niche overlap.In addition, the building of flora
It is carried out in open environment, the simple culture vessel with aerator can be met the requirements, and be particularly suitable in various black smelly water
Body administers field conduct.Present invention process step compact and reasonable, low in cost, implementation easy to spread have good Environmental Effect
Benefit, economic benefit and social benefit.
The invention mainly includes providing the functional flora construction method and concrete application method of complete set, for
Indigenous microorganism in different water bodys has general applicability, is not required to be defined the conditions such as the strain of microorganism, have
Wide application value.
The functional flora construction method provided by the present invention for being suitable for black and odorous water improvement, can be widely applied to all kinds of
Black and odorous water governance process, specific construction step are as follows:
(1) indigenous microorganism samples: the acquisition of indigenous microorganism sample is carried out to the black and odorous water that needs are administered, meanwhile, it adopts
Collect seed sludge；
Wherein the black and odorous water includes the polluted-waters such as black-odor riverway, black smelly lake, the black smelly surface water of contaminated site；
Wherein the indigenous microorganism sample is the black and odorous water mud mixture, wherein micro- containing a large amount of raw water body original inhabitants
Biology, type is abundant, and as one of flora source, functional flora adds back former water body environment, flora again after constructing
It is adaptable, significant effect, and will not be because of adverse effect caused by niche overlap；
Wherein the seed sludge be sewage treatment plants aerobic system mud mixture or secondary settling tank returned sludge,
Or untreated excess sludge；It is wherein described unprocessed to refer to that possible to influence sludge strain not active by any
Processing, such as the processes such as sludge digestion, sludge dewatering, lime stabilization；Why above-mentioned sampled point is selected, main reason is that with
Up-sampling point contains a large amount of high-activity microorganisms, and type is abundant, and strain concentration is high, and activity is strong, and has good nitrification function
Can, it is easy to hot housing；Using seed sludge as one of flora source, main function is to make up black and odorous water ecosystem system
In indigenous flora activity is weaker, disadvantage that concentration is lower；
(2) functional flora under black smelly water environment is constructed:
A. nutrient solution is prepared: nutrient solution prescription are as follows: 0.1-0.8%w/v nutritive salt, 35-85%v/v wait administering black smelly water
Body, 0.1-2%w/v inorganic carrier, surplus are tap water；The above ingredient is being furnished with the training for being aerated facility and being furnished with biologic packing material
It supports in container and is uniformly mixed；
B. be inoculated with: the above-mentioned nutrient solution volume that the step uses is estimated total volume of culture (function bacterium of completion to be built
The liquor capacity of group) 20-40%, add and ammonium salt and be uniformly mixed in nutrient solution, nutrient solution ammonia nitrogen concentration is made to reach 10-
40mg/L adjusts pH to 7.5-9.0, by the indigenous microorganism sample acquired in above-mentioned steps (1) and seed sludge according to 2:1~
In the weight ratio access culture vessel of 5:1, inoculum concentration is calculated as 2-7g/L according to sludge total concentration after inoculation with sludge dry mass
It is calculated；Wherein the specific value of estimated total volume of culture with no restriction, as long as can ensure that functional flora solution is being administered
Stage for the first time add use after, remaining volume be not less than add for the first time using front volume 30%；
C. level-one culture: aeration culture is carried out to the culture solution after above-mentioned inoculation, cultivates aeration condition: control DO:2-
4mg/L, temperature: 15-37 DEG C, pH:7.5-9.0, detection mode: every 8-24h detection culture ammonia nitrogen, nitrite nitrogen content and
pH；First stage ammonia nitrogen concentration is 10-40mg/L, whenever ammonia nitrogen, nitrite nitrogen removal rate reach 90% or more, is mended with ammonium salt
Fill ammonia nitrogen to the stage concentration, 2-4 period of continuous culture (calculation method of a cycle: with ammonium salt supplement ammonia nitrogen to this
Until ammonia nitrogen, nitrite nitrogen removal rate reach 90% or more, which is a week for culture after one stage concentration requires
Phase)；Second stage improves ammonia nitrogen concentration to 40-100mg/L with ammonium salt, ammonia nitrogen is detected and supplemented by above-mentioned standard, continuous culture
2-4 period (calculation method of a cycle: is supplemented after ammonia nitrogen is required to the second stage concentration with ammonium salt and is cultivated until ammonia
Nitrogen, nitrite nitrogen removal rate reach 90% or more, which is a cycle)；Phase III improves ammonia nitrogen with ammonium salt
Concentration is detected and is supplemented by above-mentioned standard ammonia nitrogen to 100-200mg/L, continuously cultivate 3-5 period (the calculating side of a cycle
Method: culture after ammonia nitrogen is required to the phase III concentration is supplemented with ammonium salt until ammonia nitrogen, nitrite nitrogen removal rate reach 90%
More than, which is a cycle)；Entire level-one incubation includes three phases, in each of entire level-one culture
In each period in stage, whenever pH is down to 6.5 hereinafter, adjusting pH to 7.5-9.0 with lye；
D. second level culture: the culture solution that above-mentioned steps C is obtained is stood, and the upper layer for discarding nutrient solution volume 50-80% is clear
Liquid is prepared fresh nutrient solution by formula described in step A and is added in culture vessel, and nutrient solution volume is made to reach estimated total culture
Volume adjusts ammonia nitrogen to 100-200mg/L, aeration culture, condition of culture (control DO:2-4mg/L, temperature: 15-37 with ammonium salt
DEG C, pH:7.5-9.0) and condition of the detection mode (every 8-24h detection culture ammonia nitrogen, nitrite nitrogen content and pH) with step C
It is identical, whenever ammonia nitrogen, nitrite nitrogen removal rate reach 90% or more, with ammonium salt supplement ammonia nitrogen to 100-200mg/L, continuous training
Support 3-5 period (calculation method of a cycle: with ammonium salt supplement after ammonia nitrogen is required to the stage concentration culture up to ammonia nitrogen,
Nitrite nitrogen removal rate reaches 90% or more, which is a cycle), functional flora building is completed, general to pass through
3-5 period culture solution drop ammonia nitrogen rate of above-mentioned continuous culture is not less than 100mg/ (L.d)；Entire second level incubation, whenever
PH is down to 6.5 hereinafter, adjusting pH to 7.5-9.0 with lye.
In above-mentioned steps A, the nutrition salt component composition and relative scale are by weight are as follows: NaHCO38-32 parts,
K2HPO46.5-26 parts, NaCl1.5-6 parts, MgSO40.15-0.6 parts, FeSO40.15-0.6 parts；Using the nutrition of this composition
Salt can solve the single problem of black smelly water constituent, and the hot housing for nitrifying bacteria community in indigenous microorganism and seed sludge provides
Necessary nutriment accelerates the building of functional flora, wherein NaHCO3Inorganic carbon source needed for can provide nitrobacteria growth,
Promote its mass propagation.
In above-mentioned nutrient solution prescription, the inorganic carrier used is in the common inorganic carrier such as zeolite powder, diatomite, calcium carbonate
One or more of combinations, preferred zeolite powder；The addition of inorganic carrier can provide place for attached growth of microorganisms, to mention
High flora concentration, and why preferred zeolite powder, be because zeolite powder can not only as carrier for flora apposition growth, also have
There is excellent suction ammonia ability, the nitrifying bacteria community of its surface attachment will be supplied to degrade after ammonia nitrogen absorption, the training of nitrifying bacteria community can be improved
Treatment effeciency after supporting efficiency and investment application；In addition, there are also the works of suspended matter in absorption black and odorous water for the presence of inorganic carrier
With further purifying water body environment.
In the building process of above-mentioned compounding process and follow-up function flora, certain proportion waits administering in nutrient solution
Black smelly water is added to committed step, and the building process of functional flora can be made to be in former black and odorous water aquatic environment always, made
Functional flora, which is treated, administers the good adaptability of water body holding, is particularly advantageous to improve the nitrifier more sensitive to environmental change
Adaptability of the group when cultivating and adding application, make its always can under former black and odorous water condition of water quality efficient degradation ammonia
Nitrogen, and make its strain composition with the dynamic equilibrium shape of incubation and the former black and odorous water aquatic environment variation holding survival of the fittest
State；Therefore, no matter for which kind of black and odorous water, the step for being added wait administer black smelly water, may insure constructed functional flora
Always there is good applicability and specific aim, it can be made to adapt to the application environment corresponding to it respectively.
It is above-mentioned for prepare nutrient solution process, and the building of the process and entire functional flora in open environment into
Row is not required to the operation such as sterilizing, and there is no need to special installations, can be met using the simple culture vessel with aerator and be wanted
It asks, equipment cost is cheap, such as has ready conditions, and the dedicated culture device such as fermentor, fermentation vat can also be used.
In above-mentioned culture vessel, the biologic packing material of arrangement can be soft cellulose filler, semi soft packing, shield formula filler,
The sewage treatment fields such as biological cotton net commonly fix filler；Inorganic carrier and biologic packing material are used cooperatively, on the one hand can
The cell concentration of slow growth, nitrobacteria with apposition growth characteristic is greatly increased, enhancing adds application effect, another party
Face, the strain being attached on fixed biologic packing material can guarantee that the bacterium amount in culture vessel is in higher level, Bu Huiyin always
Adding and adding and causing being greatly decreased for bacterium amount when the later period is applied helps to carry out making when supplement culture after each
Functional flora restores rapidly optimum state.
In B. seeded process, since community construction process is amplified step by step, so initial nutrient solution volume accounts for and expects
The ratio of total volume of culture cannot be excessively high, it is generally preferable in 20-40%；The ratio of indigenous microorganism sample and seed sludge is 2:
1~5:1, flora source is based on indigenous microorganism, supplemented by seed sludge；Using indigenous microorganism as flora source, institute
The functional flora of building treat administer water body adaptability it is good, biological safety is good；Supplemented by seed sludge, main reason is that connecing
Kind sludge belongs to inoculating microbe, and adaptability is limited, but its activity and strain concentration are higher, therefore is conducive to accelerate the whole of flora
Body constructs process and improves overall activity.
In C. level-one culture, being carried out using the increased mode of ammonia-nitrogen content gradient, each gradient is kept for multiple periods, and
And do not add any organic carbon source, it is therefore intended that culture environment is gradually changed into the environment of low organic carbon, high ammonia nitrogen, is quickly washed in a pan
The heterotrophism miscellaneous bacteria that can not adapt to the environment in indigenous microorganism and seed sludge is eliminated, makes nitrifying bacteria community therein black smelly wait administer
It is gradually adapted in water environment and behavior of high-strength ammonium of degrading, mass propagation becomes dominant microflora.
In D. second level culture, the supernatant liquor of level-one nutrient solution volume 50-80% is discarded, is because by macrocyclic
Level-one culture may have accumulated the metabolite for largely having inhibiting effect to microorganism growth in culture solution, need to discharge it
Culture solution；Meanwhile culture solution spreading cultivation to estimated total volume of culture, the nitre of dominant microflora will be had become by being equivalent in level-one culture
Change flora and be used as strain, fast-propagation to the total amount for meeting requirement, this process can further promotion functions microorganism
Expand numerous, superseded miscellaneous bacteria.
In above-mentioned steps B-D, adjusting lye used in pH is the sodium carbonate of any concentration, sodium bicarbonate aqueous solution or both
Mixed solution (mass ratio is unlimited when mixing), which is not only able to adjust the weak base that needs to nitrifying bacteria community of culture solution pH
Property environment, basicity needed for supplementing, additionally it is possible to play the role of the inorganic carbon source of supplement, promote nitrifying bacteria community growth and breeding, therefore be
Construct most suitable lye when the functional flora based on nitrifying bacteria community.
In above-mentioned steps B-D, adjusting ammonium salt used in ammonia nitrogen is the cheap ammonium salt such as ammonium sulfate, ammonium chloride or ammonium carbonate, carbon
Sour hydrogen ammonium etc. can provide the combination of one or more of ammonium salt of inorganic carbon source and nitrogen source simultaneously, mass ratio when multiple combinations
It is unlimited.
In above-mentioned steps B-D, the terminal that each cultivation cycle terminates is reached by ammonia nitrogen and nitrite nitrogen removal rate
Determined to 90% or more, this decision procedure is different from existing the relevant technologies, and existing majority is using degradation of ammonia nitrogen as mesh
Bacteria group culture and microbial inoculum technology of preparing all only using ammonia nitrogen degradation situation as criterion, but the accumulation of nitrite nitrogen is not only
Bacteria group culture is caused to inhibit, the culture solution containing nitrite nitrogen, which is added, also will cause secondary pollution into water body, therefore this hair
It is bright that cultivation cycle terminal is determined by ammonia nitrogen and nitrite nitrogen dual indexes.
The building process of above-mentioned functional flora, strain composition keep excellent with incubation time and the variation of raw water body aquatic environment
Win the bad dynamic balance state eliminated, ultimately form the metastable efficient functional flora to raw water body with adaptability,
The advantage of the above method also resides in, can also be with the culture of flora for the function strain that traditional biological technology can not be separately cultured
Process advantages growth reduces the cost of Spawn incubation to utmostly play the processing capacity of indigenous microorganism.
It is suitable for after the functional flora for administering black and odorous water being obtained by above-mentioned construction method, i.e., using function
Flora administers black and odorous water, and steps are as follows for specific improvement:
(1) operation is added:
The functional flora (solution) that above-mentioned building is completed is thrown by the dosage of the 100-1000ppm of water body volume to be administered
Add and (accounts for the score of water body to be administered in concentration calculation with the aqueous liquids that functional flora is added to be calculated)；Meanwhile it adopting
Water body DO to 2-4mg/L is improved with aerator；
It is above-mentioned to add in operational process, it is in functional flora to drop ammonia nitrogen using the reason of aerator raising water body DO
The nitrifying bacteria community that solution plays a major role belongs to aerobic bacteria, and DO is generally lower in black and odorous water, therefore improves water body DO by aeration,
Be conducive to the functional flora added and colonize simultaneously dominant growth in systems；
(2) it periodically adds and persistently cultivates:
A. it periodically adds: after adding for the first time, every the data that 10-30d periodic monitoring is administered, when unfinished governance for reaching standard
Target when, require supplementation with and add primary, add functional flora by the dosage of the 100-1000ppm of water body volume to be administered
(solution)；
B. it persistently cultivates: constructing the functional flora of completion and adding (including add for the first time and periodically add) use every time
Afterwards, it is required to carry out supplement culture, the method for supplementing culture is that will match according to the nutrient solution in (2) the step of constructing function flora
Side prepare fresh nutrient solution and add in culture vessel (culture vessel is culture vessel used in the community construction stage, contain
Have the functional flora for adding and periodically adding for the first time), the dosage of nutrient solution adds or periodically adds for the first time function with corresponding
Flora liquor capacity is identical, i.e., the volume and the volume of the functional flora solution added for the first time of culture solution are supplemented after adding for the first time
It is identical, after certain periodically adds functional flora solution, it is molten to add the functional flora that the volume of nutrient solution is periodically added with this
The volume of liquid is identical, and black smelly water ratio contained in nutrient solution is maintained at the 35-85%v/v of nutrient solution volume, later according to structure
The condition of culture (control DO:2-4mg/L, temperature: 15-37 DEG C, pH:7.5-9.0) of step D in the step of building functional flora (2),
Detection mode (every 8-24h detection culture ammonia nitrogen, nitrite nitrogen content and pH), feed profile carry out long-term continuous culture, but
Culture solution is no longer replaced, until adding use next time, the mode continuously cultivated for a long time is that ammonia nitrogen is adjusted with ammonium salt to 100-
200mg/L, whenever ammonia nitrogen, nitrite nitrogen removal rate reach 90% or more, with ammonium salt supplement ammonia nitrogen to 100-200mg/L, company
The 3-5 period of continuous culture (calculation method of a cycle: is supplemented after ammonia nitrogen is required to the stage concentration with ammonium salt and is cultivated until ammonia
Nitrogen, nitrite nitrogen removal rate reach 90% or more, the incubation time be a cycle) and the period in culture solution drop ammonia nitrogen rate
Not less than 100mg/ (L.d), incubation is completed；Entire incubation, whenever pH be down to 6.5 hereinafter, with lye adjust pH to
7.5-9.0；
C. terminal point determining: after functional flora adds, black and odorous water ammonia-nitrogen content is persistently measured, if in the feelings for not adding flora
The Water quality ammonia nitrogen stably reaching standard of continuous 2 months or more periodic monitorings under condition, then functional flora within the system can dominant growth,
It can stop cultivating and adding, it is on the contrary then continue to carry out according to the method described above to continue culture and periodically add.
In above-mentioned steps A, functional flora adds once every 10-30d supplement, and reason is that nitrobacteria is autotrophy
Bacterium, growth in water body environment and to colonize speed slower, therefore need to continue measurement ammonia nitrogen degradation effect after adding for the first time to go forward side by side
Row supplement adds, and effectively maintains the superiority wait administer functional flora in water body.
In above-mentioned steps B, waits administering black smelly water containing 35-85%v/v in the fresh medium of supplement, supplement each time
Culture, is carried out in current black smelly water environment, is conducive to make the strain in functional flora to form with former water quality ring
Border variation keeps the dynamic balance state of the survival of the fittest, forms the metastable efficient function to raw water body with adaptability
Energy flora, guarantee continue efficient degradation effect.
In above-mentioned steps C, if continuous 2 months or more the Water quality ammonia nitrogen stably reaching standards in the case where not adding flora,
Illustrate to have formed stable ammonia nitrogen degradation flora wait administer in water body environment, water body micro-ecological environment is successfully improved, reaches
The terminal that functional flora is administered.
Using the method for above-mentioned removal black and odorous water ammonia nitrogen, black and odorous water administer after ammonia nitrogen removal frank can reach 90% with
On, meanwhile, there is certain removal effect to water body recovery technology, BOD, COD, the organoleptic indicators such as water body chroma, turbidity, smell are bright
It is aobvious to improve.This method equipment and operating cost are cheap, are particularly suitable for administering field conduct in various black and odorous waters.
Compared with prior art, the invention has the following advantages that
1, black and odorous water is administered using the present invention, ammonia nitrogen removal frank can reach 90% or more, water body chroma, turbidity, smell
Equal organoleptic indicators are obviously improved, and have certain removal effect to water body recovery technology, BOD, COD, can improve black smelly water ring comprehensively
Border；
2, the present invention administers the function that scene utilizes indigenous microorganism and seed sludge to establish complete set in black and odorous water
Flora " continuously cultivate-periodically adds " integrated process, persistently strengthens ammonia nitrogen removal effect, water body to be administered is promoted to form stabilization
Ammonia nitrogen degradation flora, have good environmental benefit and exploitativeness；
3, the present invention utilizes indigenous microorganism and seed sludge constructing function flora, and efficient bacterium concentration is high, adaptable,
After adding can quick acting and stablize persistently, operating cost is cheap；
4, the building of functional flora of the present invention does not depart from former black and odorous water aquatic environment, and strain is formed with incubation time and original
Water quality environmental change keeps the dynamic balance state of the survival of the fittest, ultimately form it is metastable have to raw water body it is good
The efficient functional flora of adaptability, engineer application significant effect；
5, the present invention saves microorganism collection step, and application is directly added under optimum activity state, and save the cost guarantees to live
Property；
6, the building of functional flora of the present invention carries out in open environment, the operation such as sterilizing is not required to, without increasing special set
Standby, the simple culture vessel with aerator can be met the requirements, and equipment and operating cost are cheap；
7, the present invention sufficiently uses immobilized microorganism technology, acclimatization technology, biological reinforcing technology, each processing step design
Rationally, easy to operate, there is general applicability for different black and odorous waters, be not required to limit the specified strain composition of microorganism
It is fixed, it is with a wide range of applications.
Detailed description of the invention
Fig. 1 is the process flow chart of removal black and odorous water ammonia nitrogen method of the present invention.
Fig. 2 is the present invention and the effect contrast figure using the commercially available microbial inoculum progress black and odorous water improvement without domestication.
Specific embodiment
The specific embodiment of form by the following examples does further specifically above content of the invention
It is bright, but the range that this should not be interpreted as to the above-mentioned theme of the present invention is only limitted to example below.It is all to be based on above content of the present invention
The technology realized all belongs to the scope of the present invention.
Certain the black-odor riverway administration project of embodiment 1
Certain black-odor riverway is severe black and odorous water, ammonia nitrogen 16mg/L, SS100mg/L, BOD45mg/L, COD130mg/L.?
It in governance process, once spends more than 200 ten thousand yuan and buys certain import microbial inoculum, after adding operation 3 months, effect is still unobvious.The project
Functional flora building is carried out using the functional flora construction method provided by the invention for being suitable for black and odorous water improvement, is then carried out
Black and odorous water is administered, and the specific implementation is as follows:
The present embodiment provides a kind of functional flora construction methods for being suitable for black and odorous water improvement, as shown in Figure 1, specific packet
Include following steps:
(1) indigenous microorganism samples: acquiring the black-odor riverway mud mixture as indigenous microorganism sample, acquires the ground
Area's sewage treatment plants secondary settling tank returned sludge is as seed sludge；
(2) functional flora under black smelly water environment is constructed:
A. nutrient solution is prepared, is formulated are as follows: 0.8%w/v nutritive salt, 60%v/v wait administering black smelly water, 1.2%w/v diatom
Soil, surplus are tap water；
Nutritive salt at being grouped as and relative weight proportions number are as follows: NaHCO38 parts, K2HPO416 parts, NaCl6 parts,
MgSO40.6 part, FeSO40.15 part；
The above ingredient is uniformly mixed in the culture vessel for being furnished with aeration facility and being furnished with biological cotton net；
B. it is inoculated with: preparing 40% that nutrient solution is estimated total volume of culture, ammonium sulfate is added in nutrient solution and mix equal
It is even, so that nutrient solution ammonia nitrogen concentration is reached 25mg/L, adjusts pH to 9.0, the indigenous microorganism sample and seed sludge of acquisition are pressed
According in the weight ratio access culture vessel of 5:1, inoculum concentration is calculated as 2g/L according to sludge total concentration after inoculation with sludge dry mass；
C. in level-one incubation, aeration culture is carried out to the culture solution after above-mentioned inoculation, control DO is 4mg/L, temperature
It is 15 DEG C, pH 9.0, every 8h detection culture ammonia nitrogen, nitrite nitrogen content and pH；First stage ammonia nitrogen concentration is 25mg/L,
Whenever ammonia nitrogen, nitrite nitrogen removal rate reach 90% or more, with ammonium sulfate supplement ammonia nitrogen to 25mg/L, 2 week of continuous culture
Phase；Second stage improves ammonia nitrogen concentration to 70mg/L, and ammonia nitrogen is detected and supplemented by above-mentioned standard, continuously cultivates 2 periods；Third
Stage improves ammonia nitrogen concentration to 150mg/L, and ammonia nitrogen is detected and supplemented by above-mentioned standard, continuously cultivates 4 periods；Entirely cultivated
Journey, whenever pH is down to 6.5 hereinafter, adjusting pH to 9.0 with sodium carbonate；
D. in second level incubation, above-mentioned culture solution is stood, discards the supernatant liquor of nutrient solution volume 50%, is prepared new
Fresh nutrient solution simultaneously adds in culture vessel, and nutrient solution volume is made to reach estimated total volume of culture, adjusts ammonia nitrogen to 150mg/L,
Aeration culture, whenever ammonia nitrogen, nitrite nitrogen removal rate reach 90% or more, supplement ammonia nitrogen to 150mg/L is continuous to cultivate 3
Period, culture solution drop ammonia nitrogen rate reach 100mg/ (L.d) or more, and functional flora building is completed；Entire incubation, whenever pH
6.5 are down to hereinafter, adjusting pH to 9.0 with sodium carbonate.
The present embodiment also provides a kind of method that the functional flora using building administers black and odorous water, as shown in Figure 1, specifically
Including the following steps:
(1) will building complete functional flora (culture solution containing functional flora that above-mentioned steps D is finally obtained) press to
The dosage for administering the 100ppm of water body volume adds；Meanwhile water body DO to 2mg/L is improved using aerator；
(2) after adding for the first time, every 20d sample detection water body ammonia nitrogen, by water body volume to be administered when not up to standard
The dosage of 100ppm adds functional flora (culture solution containing functional flora that above-mentioned steps D is finally obtained)；It adds every time
After use, supplement culture is carried out, culture solution is made to reach total volume of culture before adding, and makes the black and odorous water ratio in nutrient solution
Example is maintained at the 60%v/v of total volume of culture；Later according to the (control of the condition of culture of step D in (2) the step of constructing function flora
DO:4mg/L processed, temperature: 15 DEG C, pH:9.0), detection mode (every 8h detection culture ammonia nitrogen, nitrite nitrogen content and pH), mend
Material mode carries out long-term continuous culture, adjusts ammonia nitrogen to 150mg/L, whenever ammonia nitrogen, nitrite nitrogen removal rate reach with ammonium sulfate
To 90% or more, ammonia nitrogen is supplemented to 150mg/L with ammonium sulfate, continuously cultivates 3 period (calculation methods of a cycle: with ammonium
Salt supplements culture after ammonia nitrogen is required to the stage concentration until ammonia nitrogen, nitrite nitrogen removal rate reach 90% or more, the culture
Time is a cycle) and the period in culture solution drop ammonia nitrogen rate be not less than 100mg/ (L.d), complete incubation；Entire training
The process of supporting, whenever pH is down to 6.5 hereinafter, adjusting pH to 9.0 with sodium carbonate；
At operation 4 months, it can be realized continuous 2 months or more Water quality ammonia nitrogen stabilizations in the case where not adding flora and reached
Mark stops culture and adds, and governance process is completed.
It is (unit mg/L) as shown in the table that front and back water quality situation is administered using this method:
| Index | Ammonia nitrogen | SS | BOD | COD |
| Before improvement | 16 | 100 | 45 | 130 |
| After improvement | 1.2 | 10 | 10 | 40 |
By detection data it is found that the black and odorous water ammonia nitrogen removal frank reaches 92.5%, SS, BOD, COD after operation 4 months
It significantly reduces, water quality clear free from extraneous odour, black smelly water environment improves comprehensively.
Certain the black smelly lake treatment project of embodiment 2
Certain black smelly lake is severe black and odorous water, ammonia nitrogen 18.9mg/L, SS29.2mg/L, BOD57.1mg/L, DO1.9mg/
L.The black smelly lake treatment project is administered using method provided by the invention, and the specific implementation is as follows:
The present embodiment provides a kind of functional flora construction methods for being suitable for black and odorous water improvement, as shown in Figure 1, specific packet
Include following steps:
(1) indigenous microorganism samples: acquiring the black smelly lake mud mixture as indigenous microorganism sample, acquires the ground
Sewage treatment plants untreated excess sludge in area's is as seed sludge；
(2) functional flora under black smelly water environment is constructed:
A. nutrient solution is prepared, is formulated are as follows: 0.1%w/v nutritive salt, 85%v/v wait administering black smelly water, 0.1%w/v carbonic acid
Calcium, surplus are tap water；
Nutritive salt at being grouped as and relative weight proportions number are as follows: NaHCO316 parts, K2HPO426 parts, l4 parts of NaC,
MgSO40.4 part, FeSO40.6 part；
The above ingredient is uniformly mixed in the culture vessel for being furnished with aeration facility and being furnished with soft cellulose filler；
B. it is inoculated with: preparing 30% that nutrient solution is estimated total volume of culture, ammonium hydrogen carbonate is added in nutrient solution and is mixed
Uniformly, so that nutrient solution ammonia nitrogen concentration is reached 10mg/L, pH to 7.5 is adjusted, by the indigenous microorganism sample and seed sludge of acquisition
According in the weight ratio access culture vessel of 4:1, inoculum concentration is calculated as 7g/ according to sludge total concentration after inoculation with sludge dry mass
L；
C. in level-one incubation, aeration culture is carried out to the culture solution after above-mentioned inoculation, control DO is 3mg/L, temperature
It is 26 DEG C, pH 7.5, every 12h detection culture ammonia nitrogen, nitrite nitrogen content and pH；First stage ammonia nitrogen concentration is 10mg/L,
Whenever ammonia nitrogen, nitrite nitrogen removal rate reach 90% or more, with ammonium hydrogen carbonate supplement ammonia nitrogen to 10mg/L, continuous culture 4
Period；Second stage improves ammonia nitrogen concentration to 40mg/L, and ammonia nitrogen is detected and supplemented by above-mentioned standard, continuously cultivates 4 periods；The
Three stages improved ammonia nitrogen concentration to 100mg/L, and ammonia nitrogen is detected and supplemented by above-mentioned standard, continuously cultivates 5 periods；Entire culture
Process, whenever pH is down to 6.5 hereinafter, adjusting pH to 7.5 with sodium bicarbonate and sodium carbonate (molar ratio 1:1)；
D. in second level incubation, above-mentioned culture solution is stood, discards the supernatant liquor of nutrient solution volume 80%, is prepared new
Fresh nutrient solution simultaneously adds in culture vessel, and nutrient solution volume is made to reach estimated total volume of culture, adjusts ammonia nitrogen to 100mg/L,
Aeration culture, whenever ammonia nitrogen, nitrite nitrogen removal rate reach 90% or more, supplement ammonia nitrogen to 100mg/L is continuous to cultivate 4
Period, culture solution drop ammonia nitrogen rate reach 100mg/ (L.d) or more, and functional flora building is completed；Entire incubation, whenever pH
6.5 are down to hereinafter, adjusting pH to 7.5.
The present embodiment also provides a kind of method that the functional flora using building administers black and odorous water, as shown in Figure 1, specifically
Including the following steps:
(1) will building complete functional flora (culture solution containing functional flora that above-mentioned steps D is finally obtained) press to
The dosage for administering the 500ppm of water body volume adds；Meanwhile water body DO to 4mg/L is improved using aerator；
(2) after adding for the first time, every 30d sample detection water body ammonia nitrogen, by water body volume to be administered when not up to standard
The dosage of 500ppm adds functional flora (culture solution containing functional flora that above-mentioned steps D is finally obtained)；It adds every time
After use, supplement culture is carried out, culture solution is made to reach total volume of culture before adding, and makes the black and odorous water ratio in nutrient solution
Example is maintained at the 85%v/v of total volume of culture；Later according to the (control of the condition of culture of step D in (2) the step of constructing function flora
DO:3mg/L processed, temperature: 26 DEG C, pH:7.5), detection mode (every 12h detection culture ammonia nitrogen, nitrite nitrogen content and pH),
Feed profile carries out long-term continuous culture, adjusts ammonia nitrogen to 100mg/L, whenever ammonia nitrogen, nitrite nitrogen removal rate with ammonium hydrogen carbonate
Reach 90% or more, ammonia nitrogen is supplemented to 100mg/L with ammonium hydrogen carbonate, continuously cultivates 4 period (calculating sides of a cycle
Method: with culture after ammonium salt supplement ammonia nitrogen to stage concentration requirement until ammonia nitrogen, nitrite nitrogen removal rate reach 90% or more,
The incubation time be a cycle) and the period in culture solution drop ammonia nitrogen rate be not less than 100mg/ (L.d), complete incubation；
Entire incubation, whenever pH is down to 6.5 hereinafter, adjusting pH to 7.5 with sodium bicarbonate and sodium carbonate (molar ratio 1:1)；
At operation 5 months, it can be realized continuous 2 months or more Water quality ammonia nitrogen stabilizations in the case where not adding flora and reached
Mark stops culture and adds, and governance process is completed.
It is (unit mg/L) as shown in the table that front and back water quality situation is administered using this method:
By detection data it is found that the black and odorous water ammonia nitrogen removal frank reaches 94.1%, and SS, BOD are significant after operation 5 months
It reduces, DO is increased, and water quality clear free from extraneous odour reaches surface water I V class standard, and black smelly water environment improves comprehensively.
The black smelly surface water administration project of certain contaminated site of embodiment 3
During certain contaminated site repairing and treating, the black smelly water pollution of earth's surface retention is serious at one, and ammonia nitrogen reaches 225mg/L,
The project water processing establishment administers ammonia nitrogen without obvious removal effect, therefore using method provided by the invention, specific real
It is as follows to apply situation:
The present embodiment provides a kind of functional flora construction methods for being suitable for black and odorous water improvement, as shown in Figure 1, specific packet
Include following steps:
(1) indigenous microorganism samples: acquiring the black smelly surface water mud mixture as indigenous microorganism sample, acquisition should
Regional sewage treatment plants aerobic system mud mixture is as seed sludge；
(2) functional flora under black smelly water environment is constructed:
A. prepare nutrient solution, be formulated are as follows: 0.5%w/v nutritive salt, 35%v/v wait administering black smelly water, 2%w/v zeolite powder,
Surplus is tap water；
Nutritive salt at being grouped as and relative weight proportions number are as follows: NaHCO332 parts, K2HPO46.5 parts, NaCl1.5 parts,
MgSO40.15 part, FeSO40.4 part；
The above ingredient is uniformly mixed in the culture vessel for being furnished with aeration facility and being furnished with semi soft packing；
B. it is inoculated with: preparing 20% that nutrient solution is estimated total volume of culture, ammonium chloride is added in nutrient solution and mix equal
It is even, so that nutrient solution ammonia nitrogen concentration is reached 40mg/L, adjusts pH to 8.5, the indigenous microorganism sample and seed sludge of acquisition are pressed
According in the weight ratio access culture vessel of 2:1, inoculum concentration is calculated as 5g/L according to sludge total concentration after inoculation with sludge dry mass；
C. in level-one incubation, aeration culture is carried out to the culture solution after above-mentioned inoculation, control DO is 2mg/L, temperature
It is 37 DEG C, pH 8.5, per the ammonia nitrogen of detection culture for 24 hours, nitrite nitrogen content and pH；First stage ammonia nitrogen concentration is 40mg/L,
Whenever ammonia nitrogen, nitrite nitrogen removal rate reach 90% or more, with ammonium chloride supplement ammonia nitrogen to 40mg/L, 3 week of continuous culture
Phase；Second stage improves ammonia nitrogen concentration to 100mg/L, and ammonia nitrogen is detected and supplemented by above-mentioned standard, continuously cultivates 3 periods；The
Three stages improved ammonia nitrogen concentration to 200mg/L, and ammonia nitrogen is detected and supplemented by above-mentioned standard, continuously cultivates 3 periods；Entire culture
Process, whenever pH is down to 6.5 hereinafter, adjusting pH to 8.5 with sodium bicarbonate；
D. in second level incubation, above-mentioned culture solution is stood, discards the supernatant liquor of nutrient solution volume 70%, is prepared new
Fresh nutrient solution simultaneously adds in culture vessel, and nutrient solution volume is made to reach estimated total volume of culture, adjusts ammonia nitrogen to 200mg/L,
Aeration culture, whenever ammonia nitrogen, nitrite nitrogen removal rate reach 90% or more, supplement ammonia nitrogen to 200mg/L is continuous to cultivate 5
Period, culture solution drop ammonia nitrogen rate reach 100mg/ (L.d) or more, and functional flora building is completed；Entire incubation, whenever pH
6.5 are down to hereinafter, adjusting pH to 8.5 with sodium bicarbonate.
The present embodiment also provides a kind of method that the functional flora using building administers black and odorous water, as shown in Figure 1, specifically
Including the following steps:
(1) will building complete functional flora (culture solution containing functional flora that above-mentioned steps D is finally obtained) press to
The dosage for administering the 1000ppm of water body volume adds；Meanwhile water body DO to 3mg/L is improved using aerator；
(2) after adding for the first time, every 10d sample detection water body ammonia nitrogen, by water body volume to be administered when not up to standard
The dosage of 1000ppm adds functional flora (culture solution containing functional flora that above-mentioned steps D is finally obtained)；It adds every time
After use, supplement culture is carried out, culture solution is made to reach total volume of culture before adding, and makes the black and odorous water ratio in nutrient solution
Example is maintained at the 35%v/v of total volume of culture；Later according to the (control of the condition of culture of step D in (2) the step of constructing function flora
DO:2mg/L processed, temperature: 37 DEG C, pH:8.5), detection mode (per the ammonia nitrogen of detection culture for 24 hours, nitrite nitrogen content and pH),
Feed profile carries out long-term continuous culture, adjusts ammonia nitrogen to 200mg/L, whenever ammonia nitrogen, nitrite nitrogen removal rate are equal with ammonium chloride
Reach 90% or more, with ammonium chloride supplement ammonia nitrogen to 200mg/L, continuously cultivate 5 periods (calculation method of a cycle: with
Ammonium salt supplements culture after ammonia nitrogen is required to the stage concentration until ammonia nitrogen, nitrite nitrogen removal rate reach 90% or more, the training
Support the time be a cycle) and the period in culture solution drop ammonia nitrogen rate be not less than 100mg/ (L.d), complete incubation；Entirely
Incubation, whenever pH is down to 6.5 hereinafter, adjusting pH to 8.5 with sodium bicarbonate；
At operation 3 months, it can be realized continuous 2 months or more Water quality ammonia nitrogen stabilizations in the case where not adding flora and reached
Mark stops culture and adds, and governance process is completed.During operation, treatment effect pair is carried out using the commercially available microbial inoculum without domestication
Than data are illustrated in fig. 2 shown below, by detection data it is found that after operation 3 months, using the black and odorous water ammonia of the method for the present invention improvement
Nitrogen index is stablized in 3mg/L or so, and removal rate is more than 98%, is significantly better than the commercially available microbial inoculum treatment effect without domestication, the dirt
It is up to standard to contaminate the black smelly surface water treatment in place.
These are only the preferred embodiment of the present invention, is not intended to restrict the invention, for those skilled in the art
For member, the invention may be variously modified and varied.All within the spirits and principles of the present invention, it is made it is any modification,
Equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of functional flora construction method for being suitable for black and odorous water improvement, it is characterised in that: the following steps are included:
(1) indigenous microorganism samples: the acquisition of indigenous microorganism sample is carried out to the black and odorous water that needs are administered, meanwhile, acquisition connects
Kind sludge；
(2) functional flora under black smelly water environment is constructed.
2. the functional flora construction method according to claim 1 for being suitable for black and odorous water improvement, it is characterised in that: described
Step (2) specifically includes:
A. nutrient solution is prepared；
B. it is inoculated with: adding ammonium salt in nutrient solution, the indigenous microorganism sample and seed sludge of acquisition are proportionally accessed into training
It supports in container；
C. level-one culture:
D. second level culture.
3. the functional flora construction method according to claim 1 or 2 for being suitable for black and odorous water improvement, it is characterised in that:
The indigenous microorganism sample is mud mixture in black and odorous water to be administered, and the black and odorous water includes black-odor riverway, black
Smelly lake or the black smelly surface water of contaminated site；The seed sludge is sewage treatment plants aerobic system mud mixture, two
Heavy pond returned sludge, untreated excess sludge.
4. the functional flora construction method according to claim 2 for being suitable for black and odorous water improvement, it is characterised in that: described
Nutrient solution prescription are as follows: 0.1-0.8%w/v nutritive salt, 35-85%v/v black and odorous water to be administered, 0.1-2%w/v inorganic carrier,
Surplus is tap water；The nutrition salt component composition and relative weight proportions number are as follows: NaHCO38-32 parts, K2HPO46.5-
26 parts, NaCl1.5-6 parts, MgSO40.15-0.6 parts and FeSO40.15-0.6 parts；The inorganic carrier be zeolite powder, diatomite,
One or more combination in calcium carbonate；The nutrient solution mixes in the culture vessel for being furnished with aeration facility and being furnished with biologic packing material
It closes uniformly, the biologic packing material is soft cellulose filler, semi soft packing, shield formula filler or biological cotton net.
5. the functional flora construction method according to claim 2 for being suitable for black and odorous water improvement, it is characterised in that: described
B. it is inoculated with specifically: taking nutrient solution volume is the 20-40% of estimated total volume of culture, and ammonium salt is added in nutrient solution and is mixed equal
It is even, so that nutrient solution ammonia nitrogen concentration is reached 10-40mg/L, pH to 7.5-9.0 is adjusted, by the indigenous microorganism sample of acquisition and inoculation
For sludge according in the weight ratio access culture vessel of 2:1~5:1, inoculum concentration does matter according to sludge total concentration after inoculation with sludge
Amount is calculated as 2-7g/L and is calculated.
6. the functional flora construction method according to claim 2 for being suitable for black and odorous water improvement, it is characterised in that: described
C. level-one culture specifically: aeration culture is carried out to the culture solution after inoculation, controls DO:2-4mg/L, temperature: 15-37 DEG C, pH:
7.5-9.0, every 8-24h detection culture ammonia nitrogen, nitrite nitrogen content and pH；First stage ammonia nitrogen concentration is 10-40mg/L, often
When ammonia nitrogen, nitrite nitrogen removal rate reach 90% or more, with supplement ammonia nitrogen to the stage concentration, 2-4 period of continuous culture；
Second stage improves ammonia nitrogen concentration to 40-100mg/L, whenever ammonia nitrogen, nitrite nitrogen removal rate reach 90% or more, supplements ammonia
Nitrogen continuously cultivates 2-4 period to the stage concentration；Phase III improves ammonia nitrogen concentration to 100-200mg/L, whenever ammonia nitrogen,
Nitrite nitrogen removal rate reaches 90% or more, and supplement ammonia nitrogen to the stage concentration continuously cultivates 3-5 period；Entire culture
In the process when pH is down to 6.5 or less, pH to 7.5-9.0 is adjusted.
7. the functional flora construction method according to claim 2 for being suitable for black and odorous water improvement, it is characterised in that: described
D. second level culture specifically: the culture solution obtained after level-one culture is stood, the supernatant liquor of 50-80% is discarded, is sought preparing
Nutrient solution simultaneously adds in culture vessel, and nutrient solution volume is made to reach estimated total volume of culture, adjusts ammonia nitrogen to 100- with ammonium salt
200mg/L, aeration culture, controls DO:2-4mg/L, temperature: 15-37 DEG C, pH:7.5-9.0, every 8-24h detection culture liquefied ammonia
Nitrogen, nitrite nitrogen content and pH, when ammonia nitrogen, nitrite nitrogen removal rate reach 90% or more, supplement ammonia nitrogen to 100-200mg/
L is continuously cultivated 3-5 period, and functional flora building is completed；When entire incubation pH is down to 6.5 or less, pH to 7.5- is adjusted
9.0。
8. a kind of function using the functional flora construction method building for being suitable for black and odorous water improvement described in claim 1 to 7
The method that energy flora administers black and odorous water, which is characterized in that specifically include following steps:
(1) operation is added:
It will be suitable for the function bacterium of the functional flora construction method building of black and odorous water improvement described in application claim 1 to 7
Group is added in black and odorous water to be administered, meanwhile, water body DO is improved using aerator；
(2) it periodically adds and persistently cultivates:
A. periodically add: after adding for the first time, when not up to management goal, per set time periodically adds functional flora；
B. it persistently cultivates: after each functional flora adds use, being required to carry out supplement culture, the nutrient solution of configuration is supplemented
Into culture vessel, the dosage of nutrient solution adds or periodically to add functional flora liquor capacity identical for the first time with corresponding, so
Long-term continuous culture is carried out afterwards；
C. terminal point determining: black and odorous water ammonia-nitrogen content is persistently measured, if the continuous long-time water quality in the case where not adding flora
Ammonia nitrogen stably reaching standard, then functional flora within the system can dominant growth, stop culture and add, on the contrary it is then continue return walk
Rapid A is periodically added and is cultivated.
9. the method that functional flora according to claim 8 administers black and odorous water, which is characterized in that initial in (1)
Feeding function flora is added by the dosage of the 100-1000ppm of water body volume to be administered, and improves water body using aerator
DO to 2-4mg/L；During the A. is periodically added, after adding for the first time, data are administered every 10-30d periodic monitoring, when unfinished
It when the target of governance for reaching standard, requires supplementation with and adds, dosage adds function bacterium by the 100-1000ppm of water body volume to be administered
Group.
10. the method that functional flora according to claim 9 administers black and odorous water, which is characterized in that the B. is persistently trained
In supporting, the condition of culture of the long-term cultivation are as follows: control DO:2-4mg/L, temperature: 15-37 DEG C, pH:7.5-9.0, every 8-24h
It detects and cultivates ammonia nitrogen, nitrite nitrogen content and pH, adjust ammonia nitrogen to 100-200mg/L, whenever ammonia with ammonium salt in incubation
Nitrogen, nitrite nitrogen removal rate reach 90% or more, supplement ammonia nitrogen to 100-200mg/L, 3-5 week of continuous culture with ammonium salt
Phase, when culture solution drop ammonia nitrogen rate completes incubation not less than 100mg/ (L.d) in the period；During the whole culture process when
PH is down to 6.5 hereinafter, adjusting pH to 7.5-9.0；
The nutrient solution prescription are as follows: 0.1-0.8%w/v nutritive salt, 35-85%v/v black and odorous water to be administered, 0.1-2%w/v without
Airborne body, surplus are tap water；The nutrition salt component composition and relative weight proportions number are as follows: NaHCO38-32 parts,
K2HPO46.5-26 parts, NaCl1.5-6 parts, MgSO40.15-0.6 parts and FeSO40.15-0.6 parts；The inorganic carrier is zeolite
Powder, diatomite, combination one or more in calcium carbonate；The nutrient solution is being furnished with the training for being aerated facility and being furnished with biologic packing material
It supports in container and is uniformly mixed, the biologic packing material is soft cellulose filler, semi soft packing, shield formula filler or biological cotton net.
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