CN115504581A - Application of chlorella and heterotrophic nitrification-aerobic denitrification composite microbial inoculum in serving as 3D-RBC biofilm formation inoculum and method - Google Patents

Application of chlorella and heterotrophic nitrification-aerobic denitrification composite microbial inoculum in serving as 3D-RBC biofilm formation inoculum and method Download PDF

Info

Publication number
CN115504581A
CN115504581A CN202211325193.9A CN202211325193A CN115504581A CN 115504581 A CN115504581 A CN 115504581A CN 202211325193 A CN202211325193 A CN 202211325193A CN 115504581 A CN115504581 A CN 115504581A
Authority
CN
China
Prior art keywords
rbc
inoculum
chlorella
biofilm
heterotrophic nitrification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202211325193.9A
Other languages
Chinese (zh)
Inventor
张千
陈旺
谭森文
秦树敏
龙昆
杨晨曦
赵天涛
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chongqing University of Technology
Original Assignee
Chongqing University of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chongqing University of Technology filed Critical Chongqing University of Technology
Priority to CN202211325193.9A priority Critical patent/CN115504581A/en
Publication of CN115504581A publication Critical patent/CN115504581A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
    • C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/02—Aerobic processes
    • C02F3/08—Aerobic processes using moving contact bodies
    • C02F3/082—Rotating biological contactors
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/30—Aerobic and anaerobic processes
    • C02F3/302—Nitrification and denitrification treatment
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/30—Aerobic and anaerobic processes
    • C02F3/308—Biological phosphorus removal
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Microbiology (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Ecology (AREA)
  • Botany (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

The invention discloses an application and a method of chlorella and heterotrophic nitrification-aerobic denitrification composite microbial inoculum in serving as a 3D-RBC biofilm-forming inoculum, wherein the heterotrophic nitrification-aerobic denitrification composite microbial inoculum and FACHB-9 Chlorella pyrenoidosa are prepared into mixed solution according to the volume ratio of 1-1 to 6 and inoculated to an inoculated 3D-RBC biological rotating disk, the inoculation amount is 15-20% of the effective volume of the 3D-RBC biological rotating disk, the linear speed of the disk is controlled to be 3-10m/min under illumination, the disk is cultured at the temperature of 25-30 ℃ until a symbiotic biofilm with the thickness of 1-2mm is formed on the surface of a reactor, and the symbiotic biofilm is cultured at the temperature of 25-30 DEG COD 680 The value is less than 0.5, and the film formation and the sewage treatment are completed; the inoculum biofilm formation 3D-RBC still keeps higher denitrification effect on high-concentration wastewater when the HRT is less than 24h or the online speed is less than 3.0m/min, and can improve the total phosphorus removal efficiency and reduce the energy consumption of equipment.

Description

Application of chlorella and heterotrophic nitrification-aerobic denitrification composite microbial inoculum in serving as 3D-RBC biofilm formation inoculum and method
Technical Field
The invention relates to the field of sewage treatment, in particular to application of chlorella and a heterotrophic nitrification-aerobic denitrification composite microbial inoculum as a 3D-RBC biofilm formation inoculum, and also relates to a method for treating high-concentration wastewater.
Background
The traditional three-dimensional structure biological rotating disc (hereinafter referred to as 3D-RBC) takes activated sludge as a biofilm formation inoculum, and has the problems of long biofilm formation time, poor tolerance, low treatment efficiency, long retention time and the like when treating high-concentration wastewater. In view of this, chinese patents CN109368938A and CN111484204B develop 3D-RBC based on heterotrophic nitrification-aerobic denitrification mixed bacteria biofilm formation (hereinafter referred to as bacteria biofilm formation), and compared with sludge biofilm formation 3D-RBC, bacteria biofilm formation 3D-RBC has significant advantages in terms of treatment efficiency, tolerance, biofilm formation time, and the like. However, this apparatus also has the following problems in practical use: (1) The 3D-RBC is still longer in residence time when treating high-concentration wastewater, so that the equipment is large in volume and high in cost; (2) The 3D-RBC is a biological membrane process essentially, and the residual sludge discharge is extremely low, so that the total phosphorus removal effect is very poor; (3) The microbial inoculum biofilm formation 3D-RBC has a large demand on dissolved oxygen when treating high-concentration wastewater, a biological rotating disc is required to keep a higher linear velocity to maintain the concentration of the dissolved oxygen in the wastewater, and the biomass on the disc can be obviously reduced at the higher linear velocity, so that the removal efficiency of pollutants is influenced; (4) When the microbial inoculum biofilm formation 3D-RBC is used for treating high-concentration wastewater, the rotary table is required to operate at a higher linear speed, so that the energy consumption of equipment is higher, and meanwhile, the abrasion and depreciation of the rotary shaft of the rotary table can be accelerated; (5) When the 3D-RBC is used for treating high-concentration wastewater, most of high-concentration organic matters are converted into carbon dioxide to be discharged.
Therefore, a method for treating high-concentration wastewater with short retention time, high total phosphorus removal efficiency, low equipment energy consumption and low carbon emission is urgently needed.
Disclosure of Invention
In view of the above, one of the purposes of the present invention is to provide an application of chlorella and heterotrophic nitrification-aerobic denitrification composite microbial inoculum as a biofilm formation inoculum of a 3D-RBC biological rotating disc; the invention also aims to provide a method for treating high-concentration wastewater by using chlorella and a heterotrophic nitrification-aerobic denitrification composite microbial inoculum as a biofilm formation inoculum of a 3D-RBC biological rotating disc.
In order to achieve the purpose, the invention provides the following technical scheme:
1. the chlorella and heterotrophic nitrification-aerobic denitrification compound bacterial agent is applied to 3D-RBC biological rotating disc biofilm formation inocula, and the compound bacterial agent is formed by compounding cuprum greedy bacteria (Cupriavidus sp) SWA1, alcaligenes faecalis (Alcaligenes faecalis), acinetobacter (Acinetobacter) and Ochrobactrum sp (Ochrobactrum sp) TAC-2.
Preferably, the chlorella is Chlorella pyrenoidosa FACHB-9.
Preferably, the volume ratio of the chlorella to the heterotrophic nitrification-aerobic denitrification composite microbial inoculum is 1.
2. A method for treating high-concentration wastewater by using chlorella and heterotrophic nitrification-aerobic denitrification composite bacteria as biofilm-hanging inoculants of a 3D-RBC biological rotating disk is characterized in that the heterotrophic nitrification-aerobic denitrification composite bacteria and the FACHB-9 are prepared into mixed liquor according to the volume ratio of 1-1 680 The value is less than 0.5 to finish the film formation and the sewage treatment.
More preferably, the heterotrophic nitrification-aerobic denitrification complex microbial inoculum and the FACHB-9 Chlorella pyrenoidosa are mixed according to the volume ratio of 1; the linear velocity was controlled at 5m/min.
Preferably, the concentration of each component of the high-concentration wastewater is as follows: NH (NH) 4+ N is more than or equal to 600mg/L, TN is more than or equal to 700mg/L, TP is more than or equal to 140mg/L, and COD is more than or equal to 6000-6500mg/L.
Preferably, the OD value of the heterotrophic nitrification-aerobic denitrification composite microbial inoculum is 1.0-1.4.
Preferably, the OD of the chlorella 680 Is 1.5-1.7.
The invention preferably adds the wastewater for 6 to 8 times in the treatment process and controls NH 4+ 650-680mg/L of N, 720-780mg/L of TN, 140-160mg/L of TP and 6000-6500mg/L of COD.
Preferably, the illumination is performed for 24 hours under the illumination intensity of 2000 lux.
The invention has the beneficial effects that: the chlorella and the heterotrophic nitrification-aerobic denitrification composite microbial inoculum are used as a biofilm formation inoculum of the 3D-RBC biological rotating disc, and the biofilm formation 3D-RBC still keeps higher denitrification effect on high-concentration wastewater when the HRT is less than 24h, so that the effect is superior to that the prior biofilm formation 3D-RBC which needs longer HRT (more than 36 h) for high-concentration wastewater to obtain ideal denitrification effect; in the aspect of online speed, the biofilm formation 3D-RBC still keeps higher denitrification effect on high-concentration wastewater when the online speed is less than 3.0m/min, and is superior to the prior biofilm formation 3D-RBC which needs higher linear speed (more than 7.5 m/min) for high-concentration wastewater to obtain ideal denitrification effect. And the total phosphorus removal effect of the biofilm formation 3D-RBC equipment is improved by 33.4 percent, which shows that the addition of chlorella can solve the problem of total phosphorus removal; the energy consumption of the 3D-RBC equipment for culturing the bacteria and algae to form the membrane is reduced by 34.5 percent, which shows that the energy consumption of the equipment can be obviously reduced by adding the chlorella; the carbon emission of the 3D-RBC equipment with the bacteria and algae biofilm formation is reduced by 53.2 percent, which shows that the carbon emission of the equipment can be obviously reduced by adding the chlorella.
Drawings
In order to make the purpose, technical scheme and beneficial effect of the invention more clear, the invention provides the following drawings for explanation:
FIG. 1 is a structural diagram of a three-dimensional structure biological rotating disk of the invention (1: water inlet; 2: water outlet; 3: incandescent lamp; 4: solar panel; 5: storage battery; 6: inverter; 7: low-speed motor; 8: bacteria and algae biofilm-hanging biomembrane; 9: bacteria and algae-wastewater mixed liquor);
FIG. 2 is a structural diagram of a conventional three-dimensional structure biological rotating disk (1: water inlet; 2: water outlet; 7: low-speed motor; 8: bacteria and algae biofilm-formation biological membrane; 9: bacteria agent-wastewater mixed liquor or sludge-wastewater mixed liquor; 10: power supply);
FIG. 3 is a comparison of rotational speed versus denitrification efficiency and biofilm formation start time for a 3D-RBC reactor for bacteria and algae biofilm formation;
FIG. 4 is a comparison of denitrification efficiency and biofilm formation start time of bacteria-algae comparison 3D-RBC reactor;
FIG. 5 is a comparison of denitrification effects of a microbial inoculum biofilm and a bacteria-algae biofilm 3D-RBC reactor under different HRTs;
FIG. 6 is a comparison of denitrification effect of the 3D-RBC reactor at different linear velocities.
FIG. 7 is a comparison of the phosphorus removal effect of the 3D-RBC reactor of the biofilm formation of the microbial inoculum and the biofilm formation of the bacteria and algae.
FIG. 8 is a comparison of energy consumption of a 3D-RBC reactor at different linear speeds for biofilm formation by a microbial agent and an algae biofilm formation.
FIG. 7 is a comparison result of the phosphorus removal effect of the 3D-RBC reactor of the biofilm formation of the microbial inoculum and the biofilm formation of the bacteria and algae.
FIG. 8 shows the comparison results of energy consumption of the 3D-RBC reactor at different linear velocities for biofilm formation by the microbial agents and the 3D-RBC reactor.
Detailed Description
The present invention is further described with reference to the following drawings and specific examples so that those skilled in the art can better understand the present invention and can practice the present invention, but the examples are not intended to limit the present invention.
Example 1
In the three-dimensional structure biological rotating disc disclosed in chinese patent publication No. CN111484204B, an incandescent lamp and a solar panel are added on the basis of a biological pretreatment unit, the incandescent lamp is disposed in the middle of the biological pretreatment unit, the solar panel is disposed above the biological pretreatment unit, and does not need an external power supply, and is replaced with a storage battery and an inverter, solar energy generated by the solar panel is stored in the storage battery, and is converted by the inverter to provide a lighting power supply for the incandescent lamp, and the structure is shown in fig. 1.
Meanwhile, the structure of the traditional three-dimensional structure biological rotating disc is used for comparison, and the structure is shown in figure 2. Wherein the sludge biofilm formation 3D-RBC and the microbial inoculum biofilm formation 3D-RBC are respectively added into the sludge-wastewater mixed liquor and the microbial inoculum wastewater mixed liquor.
Example 2
The three-dimensional structure biological rotating disc in the embodiment 1 is utilized to treat wastewater, the wastewater is high ammonia nitrogen livestock breeding wastewater, the ammonia nitrogen is 650-680mg/L, the total nitrogen is 720-780mg/L, the total phosphorus is 140-160mg/L, the COD is 6000-6500mg/L, and the temperature is 25-30 ℃. The effective illumination time of the photovoltaic panel of the bacteria-algae reactor is 4h, the maximum power generation power is 40W, the photoelectric conversion efficiency is 15%, the illumination intensity is 2000lux, and the illumination time is 24h; bacterial liquid Density OD 600 1.2 Chlorella OD 680 1.6, mixing bacteria and algae according to the volume of 1.
In order to study the influence of the biofilm formation condition on the denitrification efficiency and the biofilm formation start time, the rotation speed is set to be 1m/min, 3m/min, 5m/min, 7m/min, 10m/min and 15m/min respectively for treatment, and then the denitrification efficiency and the biofilm formation start time are counted, and the result is shown in FIG. 3. The result shows that the biofilm formation starting time is short and the total nitrogen removal effect is good under the condition that the rotating speed is 3-10m/min, the system denitrification effect can be greatly reduced by increasing or reducing the rotating speed, meanwhile, the biofilm formation starting time is prolonged, the optimal rotating speed is 5m/min, and the biofilm formation starting time and the total nitrogen removal effect are optimal at the moment and are respectively 18d and 62.1%, which shows that the rotating speed has the best influence on the denitrification effect and the biofilm formation starting time.
And (3) setting the ratio of bacteria to algae to be 0. The results show that the fungus-algae ratio has very significant influence on the denitrification and biofilm formation starting of the system, the biofilm formation starting time is low and the total nitrogen removal effect is good within the range of 1-1; increasing or decreasing the ratio of bacteria to algae results in significantly reduced denitrification efficiency of the system and significantly increased biofilm formation time.
After the biofilm formation is finished, the disk linear velocity is kept at 15m/min, and denitrification effect graphs of two 3D-RBC reactors under different Hydraulic Retention Time (HRT) are shown in a graph 5. The result shows that the denitrification effect of the control group is reduced sharply after the HRT is lower than 36h, while the experimental group can still maintain higher denitrification effect when the HRT is 12 h. Control NH at HRT =36h 4 + Average removal rates of-N and TN are 76.9% and 69.0%, respectively, and NH is generated when HRT =12h in the experimental group 4 + The average removal rates of-N and TN are 74.1% and 69.4% respectively, which indicates that the bacteria-algae biofilm reactor still has higher denitrification effect on high-concentration wastewater under the condition of shorter HRT.
In order to research the influence of different linear velocities on the denitrification effect. The denitrification effect of both reactors was measured while maintaining HRT =48h, while adjusting the disk linear velocity, and the results are shown in fig. 6. As a result, it was observed that the control group could ensure good denitrification effect only when the linear velocity was greater than 7.5m/min, while the experimental group still maintained good denitrification effect when the linear velocity was less than 3 m/min. NH when the linear speed of the control group is 7.5m/min 4 + The average removal rates of-N and TN are 68.7% and 60.9%, respectively, and NH is generated when the online speed of an experimental group is 3m/min 4 + The average removal rates of-N and TN are 66.6% and 61.1% respectively, and the results show that the 3D-RBC still retains higher denitrification effect under the condition of lower linear velocity.
The comparison result of the phosphorus removal effect of the 3D-RBC reactor is shown in FIG. 7. The result shows that under the condition of meeting the higher denitrification effect, the rotating speed of a selected control group is 7.5m/min, HRT =36h, the rotating speed of an experimental group is 3m/min, and HRT =24h, phosphorus removal of two groups of reactors is compared, the average removal rate of the control group on total phosphorus is 15.4%, the removal rate of the bacteria algae adopted by the experimental group on TP is obviously improved, the average removal rate of the bacteria algae is 48.7%, and the removal rate of the experiment group on TP is improved by 33.4%.
The results of energy consumption comparison between the 3D-RBC reactor with bacteria biofilm formation and the 3D-RBC reactor with bacteria biofilm formation are shown in FIG. 8. The result shows that under the condition of meeting the higher denitrification effect, the rotating speed of a selected control group is 7.5m/min, HRT =36h, the rotating speed of an experimental group is 3m/min, and HRT =24h, the energy consumption of the control group is 0.1092 KW.h under the condition, the energy consumption of the experimental group is 0.0715 KW.h, and the energy consumption is obviously reduced by 34.5%. The energy consumption was calculated as shown in table 1.
TABLE 1 CO 2 Emission reduction calculation
Device Q in (m 3 ) COD in (mg/L) Q out (m 3 ) R COD △Csm(g) R CO2 C CO2 (g)
3D-RBC (microbial inoculum) 3.6×10 -3 6000 3.6×10 -3 76.1％ 2.101×10 -2 0 5.1414
3D-RBC of bacteria and algae 3.6×10 -3 6000 3.6×10 -3 87.3％ 2.504×10 -2 13％ 2.4055
In a carbon capture and utilization device based on algal symbiosis, microorganisms synthesize new cells by using organic carbon in sewage and breathe, and when the content of the organic carbon is not enough to be discharged into the atmosphere, CO is generated through endogenous respiration 2 The light utilization efficiency of chlorella reaches 18.0%. Estimating the carbon dioxide emission of the control group, cco, according to a carbon conservation formula 2 =5.1414g; the carbon emission of the experimental group was 2.4055g. The results show that the treatment of aquaculture wastewater based on carbon capture and utilization device for symbiosis of bacteria and algae can reduce 53.2% of CO compared with microbial inoculum biofilm formation 3D-RBC 2 And (4) discharging. The addition of Chlorella can absorb part of CO via photosynthesis 2 The carbon emission generated in the process is greatly reduced, and the aims of energy conservation and emission reduction are achieved to a certain extent.
The above-mentioned embodiments are merely preferred embodiments for fully illustrating the present invention, and the scope of the present invention is not limited thereto. The equivalent substitution or change made by the technical personnel in the technical field on the basis of the invention is all within the protection scope of the invention. The protection scope of the invention is subject to the claims.

Claims (9)

1. The application of the chlorella and the heterotrophic nitrification-aerobic denitrification composite microbial inoculum in serving as a biofilm formation inoculum of a 3D-RBC biological rotating disc is characterized in that: the compound microbial inoculum is compounded by cupriopsis cuprianum (cupriopsis sp) SWA1, alcaligenes faecalis (Alcaligenes faecalis), acinetobacter (Acinetobacter) and Ochrobactrum (Ochrobactrum sp) TAC-2.
2. Use according to claim 1, characterized in that: the Chlorella is Chlorella pyrenoidosa FACHB-9.
3. Use according to claim 1, characterized in that: the volume ratio of the chlorella to the heterotrophic nitrification-aerobic denitrification composite microbial inoculum is 1 to 2-1.
4. The method for treating high-concentration wastewater by using chlorella and heterotrophic nitrification-aerobic denitrification composite microbial inoculum as a biofilm formation inoculum of a 3D-RBC biological rotating disc is characterized by comprising the following steps of: the heterotrophic nitrification-aerobic denitrification complex microbial inoculum and the FACHB-9 pyrenoidosa are prepared into a mixed solution according to the volume ratio of 1-1 680 The value is less than 0.5 to finish the film formation and the sewage treatment.
5. The biofilm culturing method for the 3D-RBC biological rotating disk biofilm culturing inoculum by utilizing the chlorella and the heterotrophic nitrification-aerobic denitrification composite microbial inoculum according to claim 4, which is characterized in that: the high-concentration wastewater comprises the following components in concentration: NH 4+ N is more than or equal to 600mg/L, TN is more than or equal to 700mg/L, TP is more than or equal to 140mg/L, and COD is more than or equal to 6000mg/L.
6. The biofilm culturing method for the 3D-RBC biological rotating disk biofilm culturing inoculum by utilizing the chlorella and the heterotrophic nitrification-aerobic denitrification composite microbial inoculum according to claim 4, which is characterized in that: the OD value of the heterotrophic nitrification-aerobic denitrification composite bacterial agent is 1.0-1.4.
7. The biofilm culturing method of chlorella and heterotrophic nitrification-aerobic denitrification complex inoculant as the biofilm culturing inoculum of the 3D-RBC biological rotating disc according to claim 4, wherein the biofilm culturing method comprises the following steps: the smallOD of coccobacillus 680 Is 1.5-1.7.
8. The biofilm culturing method of chlorella and heterotrophic nitrification-aerobic denitrification complex inoculant as the biofilm culturing inoculum of the 3D-RBC biological rotating disc according to claim 4, wherein the biofilm culturing method comprises the following steps: adding wastewater for 6-8 times in the treatment process, and controlling NH 4+ 650-680mg/L of N, 720-780mg/L of TN, 140-160mg/L of TP and 6000-6500mg/L of COD.
9. The biofilm culturing method for the 3D-RBC biological rotating disk biofilm culturing inoculum by utilizing the chlorella and the heterotrophic nitrification-aerobic denitrification composite microbial inoculum according to claim 4, which is characterized in that: the illumination is performed for 24 hours under the illumination intensity of 2000 lux.
CN202211325193.9A 2022-10-27 2022-10-27 Application of chlorella and heterotrophic nitrification-aerobic denitrification composite microbial inoculum in serving as 3D-RBC biofilm formation inoculum and method Pending CN115504581A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202211325193.9A CN115504581A (en) 2022-10-27 2022-10-27 Application of chlorella and heterotrophic nitrification-aerobic denitrification composite microbial inoculum in serving as 3D-RBC biofilm formation inoculum and method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202211325193.9A CN115504581A (en) 2022-10-27 2022-10-27 Application of chlorella and heterotrophic nitrification-aerobic denitrification composite microbial inoculum in serving as 3D-RBC biofilm formation inoculum and method

Publications (1)

Publication Number Publication Date
CN115504581A true CN115504581A (en) 2022-12-23

Family

ID=84513101

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202211325193.9A Pending CN115504581A (en) 2022-10-27 2022-10-27 Application of chlorella and heterotrophic nitrification-aerobic denitrification composite microbial inoculum in serving as 3D-RBC biofilm formation inoculum and method

Country Status (1)

Country Link
CN (1) CN115504581A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114014442A (en) * 2021-10-18 2022-02-08 哈尔滨工业大学 Bacterial-algae symbiotic folding sewage purification reactor using rare earth luminescent material as light source

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111233166A (en) * 2020-01-19 2020-06-05 重庆理工大学 Method for biofilm formation of biofilm reactor by using microbial inoculum
CN113003727A (en) * 2021-03-02 2021-06-22 重庆理工大学 Bacteria and algae composition for synergistically degrading high-concentration ammonia nitrogen and phosphate and application and method thereof
CN111484204B (en) * 2020-05-07 2022-07-12 重庆理工大学 Combined process and treatment system for treating high ammonia nitrogen wastewater
CN115491312A (en) * 2022-03-09 2022-12-20 华中科技大学 Preparation method and application of aerobic denitrifying bacteria-chlorella algae biomembrane

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111233166A (en) * 2020-01-19 2020-06-05 重庆理工大学 Method for biofilm formation of biofilm reactor by using microbial inoculum
CN111484204B (en) * 2020-05-07 2022-07-12 重庆理工大学 Combined process and treatment system for treating high ammonia nitrogen wastewater
CN113003727A (en) * 2021-03-02 2021-06-22 重庆理工大学 Bacteria and algae composition for synergistically degrading high-concentration ammonia nitrogen and phosphate and application and method thereof
CN115491312A (en) * 2022-03-09 2022-12-20 华中科技大学 Preparation method and application of aerobic denitrifying bacteria-chlorella algae biomembrane

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114014442A (en) * 2021-10-18 2022-02-08 哈尔滨工业大学 Bacterial-algae symbiotic folding sewage purification reactor using rare earth luminescent material as light source

Similar Documents

Publication Publication Date Title
CN104193003B (en) The low CN of aerobic/anaerobic SNDPR system process of enrichment polyP bacteria is than the technique of sanitary sewage
CN105565508A (en) Membrane aeration bacteria-algae biological membrane system and application thereof
CN110540292B (en) Enhanced synchronous nitrification and denitrification process for adding AHLs signal molecules into biological moving bed process
CN102730914B (en) Method for processing livestock breeding wastewater by using anoxic membrane bioreactor
CN109368938A (en) A kind of full aerobic nitrogen removal technique of livestock breeding wastewater pre-treatment and integration apparatus
CN111138038B (en) Photovoltaic-driven rural domestic sewage integrated treatment system and treatment method
CN103288213A (en) Whole-process autotrophic nitrogen removal method and device
CN103773726A (en) Method for enriching and acclimating denitrifying phosphate-accumulating organisms at low temperature
CN113003727A (en) Bacteria and algae composition for synergistically degrading high-concentration ammonia nitrogen and phosphate and application and method thereof
CN103359827A (en) Fast starting method of MBR (Membrane bioreactor) autotrophic nitrogen removal technique, and method for synchronously removing carbonic nitrogen in domestic sewage by utilizing same
CN108383239B (en) Integrated biological treatment process for shortcut nitrification anaerobic ammonia oxidation and phosphorus removal under intermittent aeration mode
CN115504581A (en) Application of chlorella and heterotrophic nitrification-aerobic denitrification composite microbial inoculum in serving as 3D-RBC biofilm formation inoculum and method
CN111908605A (en) Method for treating domestic sewage by anaerobic ammonia oxidation-oxidation ditch coupling
CN114873837B (en) Method and device for realizing deep denitrification and dephosphorization by PD/A in-situ coupling algae
CN110980947A (en) Denitrifying phosphorus removal sewage treatment device based on multistage anaerobism-MABR
CN103951057A (en) Normal-temperature low-C/N-ratio sewage continuous shortcut nitrification starting method
CN206232499U (en) A kind of solar energy integral type solution laminar aerator
CN112939379A (en) Advanced treatment method for industrial production wastewater of lithium battery
CN110054296B (en) A/O/A SBR process for treating low C/N ratio municipal sewage
CN115745178B (en) Method for realizing synchronous aerobic anoxic denitrification and dephosphorization by composite granular sludge system
CN111018101A (en) Membrane biofilm culture domestication process and membrane biofilm reaction device for treating high-salinity wastewater
CN107892392B (en) Low-carbon energy-saving heat preservation device and method for bacteria-algae biomembrane sewage reactor
CN113104990B (en) Method for enriching ammonia oxidizing bacteria based on inhibition of nitrite oxidizing bacteria at medium and high temperature
CN109824145B (en) Device and method for rapidly realizing autotrophic denitrification of domestic sewage by regulating and controlling flora structure
CN207121475U (en) A kind of new Carrousel oxidation ditch

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination