CN103773726A - Method for enriching and acclimating denitrifying phosphate-accumulating organisms at low temperature - Google Patents
Method for enriching and acclimating denitrifying phosphate-accumulating organisms at low temperature Download PDFInfo
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Abstract
The invention relates to the field of sewage treatment and environment protection, in particular to a method for enriching and acclimating denitrifying phosphate-accumulating organisms at a low temperature for solving the problems of difficulty in enriching and acclimating denitrifying phosphate-accumulating organisms and poor nitrogen and phosphorus removal effect of sewage at a low temperature. The method adopts the strategy of a circulating running mode of two-stage type (anaerobic/aerobiotic treatment followed by anaerobic/anaerobic treatment secondly) enriching acclimation, feeding nutritious water at high load and low load sequentially, feeding water, reacting, settling and draining, the enrichment quantity of phosphorous removal microorganisms is increased, the breeding rate of organisms is improved, and an optimal condition is created for the microorganism breeding. The denitrifying phosphate-accumulating organisms are successfully enriched and acclimated at a low temperature through 40 days anaerobic/aerobiotic operation and 30 days anaerobic/anoxic running, and the concentration of phosphorus in effluent is stabilized to be 0.5mg/L.
Description
Technical field
The present invention relates to sewage disposal and field of environment protection, specifically a kind of Denitrifying Phosphate Accumulating Organisms acclimating method under cold condition.
Background technology
Nitrogen, phosphoric are two important factors that cause body eutrophication, and there is strict restriction in numerous countries to its emission concentration.The direct result of body eutrophication is accelerated water body ager process exactly, makes water body lose the function of original fishery, tourism and drinking water source.When national environmental protection portion of China requires urban wastewater treatment firm water outlet to enter sealing, the semiclosed waters such as major river valley and lake, reservoir, carry out " urban wastewater treatment firm pollutant emission standard " one-level A standard (GB18918-2002).Along with the raising of nitrogen, phosphorus emission standard, in newly-built sewage work or old technology upgrading process, the Biological Nitrogen Removal Processe application of high-level efficiency less energy-consumption is particularly important.
At present, Enhanced Biological Phosphorus Removal technique (enhanced biological phosphorus removal, EBPR) is because its efficiency is high, cost is low and environmental friendliness enjoys focus of attention.In this technique, main functional microorganism is polyP bacteria (Accumulibacter), its principal feature is: under anaerobic poly-phosphorus hydrolysis generate energy (adenosine triphosphate in cell, and reduced coenzyme NADH ATP), for voltaile fatty acid (volatile fatty acid, VFA) absorption also saves as poly-β-hydroxybutyric acid (poly-β-hydroxybutyrate, PHB); Then under aerobic condition take PHB as electron donor, carry out excessive suction phosphorus take oxygen as electron acceptor(EA), thereby remove the phosphorus in sewage with the form of excess sludge discharge.Along with the further investigation of scholar to polyP bacteria, find that existing a kind of polyP bacteria can carry out synchronous denitrification denitrogenation dephosphorizing under anoxia condition take nitrate as electron acceptor(EA), be called Denitrifying Phosphate Accumulating Organisms.Denitrifying Phosphate Accumulating Organisms can be saved aeration rate 30% than aerobic phosphorous accumulating bacterium, reduces sludge creation amount 50%, reduces carbon source demand amount 50% and is particularly suitable for low C/N than the processing of sanitary sewage.Be that aerobic phosphorous accumulating bacterium or Denitrifying Phosphate Accumulating Organisms are all very easily subject to having similar metabolic type but do not possess the fusca xylanase competition of releasing phosphorus and inhaling phosphorus function in enrichment process, and the factors such as pH, DO, carbon source and temperature will affect the enrichment environment of polyP bacteria and fusca xylanase.In these influence factors, temperature is the major influence factors of polyP bacteria and fusca xylanase enrichment.Chinese scholars has been done certain research to the acclimating of Denitrifying Phosphate Accumulating Organisms in normal temperature (25 ± 5 ℃) situation, has obtained some achievements in research and successful experience.
But the acclimating situation of polyP bacteria, has important directive significance (especially winter) to EBPR system applies in practice under research low temperature environment.
Summary of the invention
The object of the present invention is to provide a kind of Denitrifying Phosphate Accumulating Organisms acclimating method under cold condition.
The object of the present invention is achieved like this:
(1) fill phase: utilize intake pump 2 that solution in inlet chest 1 is pumped in reactor 12, controlling flooding time by water inlet controller 5 is 30min;
(2) anaerobic stages: start blast aeration machine 3 nitrogen is blasted in reactor 12 by aeration head 11, anaerobic environment is provided, passing into the nitrogen time is 2h by aeration controller 6 controls; Start machine mixer 7 and drive stirring rake 10 to stir, the power that provides solution to mix completely, it is 2h that the mechanical stirring time is controlled by mechanical stirring controller 8;
(3) aerobic stage: after anaerobic reaction 2h completes, change reactor anaerobic environment into aerobic environment, by blast aeration machine 3, air is blasted in reactor 12 by aeration head 11, aerobic environment is provided, passing into the air time is 4h by aeration controller 6 controls; Start machine mixer 7 and drive stirring rake 10 to stir, the power that provides solution to mix completely, it is 4h that the mechanical stirring time is controlled by mechanical stirring controller 8;
(4) precipitate phase: after aerobic reaction 4h completes, aeration controller 6 is closed blast aeration machine 3 automatically, and mechanical stirring controller 8 cuts out machine mixer 7 automatically, sedimentation time is 1h;
(5) bleeding stage: after precipitation 1h completes, start wet-pit 14 precipitation supernatant liquor is entered in water tank 15, it is 30min that water discharge time is controlled by water discharge controller 9;
(6) repeating step (1), step (2), step (3), step (4), step (5), again to complete into water, anaerobism, aerobic, precipitation and drainage procedure, in these 120 cycles of process operation, amounts to 40d;
(7) after aerobic/anaerobic stage 40d completes, the running status of reactor changes the anaerobic/anoxic stage into;
(8) fill phase: same to step (1) is identical, but influent load reduces;
(9) anaerobic stages: same to step (2) is identical;
(10) the anoxic stage: after anaerobic reaction 2h completes, by adding nitrate solution to change reactor anaerobic environment into anaerobic environment, start machine mixer 7 and drive stirring rake 10 to stir, the power that provides solution to mix completely, it is 4h that the mechanical stirring time is controlled by mechanical stirring controller 8;
(11) precipitate phase: same to step (4);
(12) bleeding stage: same to step (5)
(13) repeating step (8), step (9), step (10), step (11), step (12), again to complete into water, anaerobism, anoxic, precipitation and drainage procedure, in these 90 cycles of process operation, amounts to 30d;
(14) regularly gather water outlet water sample, measure PO in water outlet
4 3--P concentration;
In the described inlet chest of step (1), solution C OD concentration is 800mg/L, PO
4 3--P concentration is 40mg/L.
The speed that the described blast aeration machine of step (2) passes into nitrogen is 1.5L/min.
The speed that the described blast aeration machine of step (3) passes into air is 1.5L/min.
In the described inlet chest of step (8), solution C OD concentration is 300mg/L, PO
4 3--P concentration is 20mg/L.
The described nitrate concentration of step (10) is 30mg/L.
The PO that step (14) is described
4 3--P method for measurement of concentration is molybdenum-antimony anti-spectrophotometric method.
Main points of the present invention are:
(1) adopt two-part acclimating Denitrifying Phosphate Accumulating Organisms, first acclimating aerobic phosphorous accumulating bacterium under aerobic/anaerobic condition, filters out dephosphorization microorganism; Acclimating Denitrifying Phosphate Accumulating Organisms under anaerobic/anoxic condition again, filters out the dephosphorization microorganism under anoxia condition.
(2) the circular flow pattern of employing water inlet, reaction, precipitation, draining, reduces the impact of nitrate on anaerobic phosphorus release, and reduces the impact of carbon source on denitrification nitrogen and phosphorus removal.
(3) adopt first high rear low water inlet loads of nutrition, in initial operating stage water inlet, loads of nutrition is high, accelerates the fast enriching of dephosphorization microorganism under low temperature environment; Loads of nutrition in reducing water inlet in the operation later stage, makes the Denitrifying Phosphate Accumulating Organisms of acclimating adapt to normal sanitary sewage.
Specific embodiment of the invention measure is:
(1) design of reactor and building: reactor design adopts sequencing batch active sludge, is mainly made up of water inlet system, aeration agitation system, outlet system and time controlled system.This device can be controlled water inlet, reaction, precipitation, drainage procedure by each controller time series, realizes automatic operating management, greatly alleviated artificial intensity, saved Pollution abatement cost, and device structure is simple, convenient mobile.
(2) aerobic/anaerobic operational mode: by sewage work's Aerobic Pond sludge seeding in this sequencing batch active sludge reactor, acclimating dephosphorization microorganism under aerobic/anaerobic environment, control water inlet in COD concentration be 800mg/L, PO
4 3--P concentration is 40mg/L, gathers its PO of water outlet water determination every day
4 3--P concentration, with PO in water outlet
4 3--P concentration stabilize and reach emission standard and determine that acclimating work completes.
(3) anaerobic/anoxic operational mode: acclimating Denitrifying Phosphate Accumulating Organisms under anaerobic/anoxic environment, controlling COD concentration in water inlet is 300mg/L, PO
4 3--P concentration is 20mg/L, gathers its PO of water outlet water determination every day
4 3--P concentration, with PO in water outlet
4 3--P concentration stabilize and reach emission standard and determine that acclimating work completes.
The present invention is applicable to the quick startup of Enhanced Biological Phosphorus Removal technique under low temperature environment, has very strong practical significance and applicability widely.
The present invention compares with domestic and international existing similar technology, its creativeness be following some:
1, studied under low temperature environment the acclimating of Denitrifying Phosphate Accumulating Organisms in Enhanced Biological Phosphorus Removal technique, for most area sewage disposal in winter qualified discharge provides may;
2, adopt first high rear low water inlet loads of nutrition, accelerated the acclimating speed of microorganism;
3, adopt the circular flow pattern of water inlet, reaction, precipitation, draining, for microbial reproduction has been created optimum condition.
The present invention is with the difference of domestic and international prior art:
1, adopt two-part (first aerobic/anaerobic, rear anaerobic/anoxic) acclimating method, strengthened the enrichment quantity of dephosphorization microorganism;
2, adopt first high rear low water inlet loads of nutrition, improve microbial reproduction speed;
3, adopt the circular flow pattern of water inlet, reaction, precipitation, draining, for microbial reproduction has been created optimum condition.
The invention has the beneficial effects as follows:
Adopt two-part (first aerobic/anaerobic, rear anaerobic/anoxic) acclimating method, strengthened the enrichment quantity of dephosphorization microorganism; Adopt first high rear low water inlet loads of nutrition, improve microbial reproduction speed; Adopt the circular flow pattern of water inlet, reaction, precipitation, draining, for microbial reproduction has been created optimum condition.By this strategy, can improve the operating performance of Enhanced Biological Phosphorus Removal technique at cold condition.
Accompanying drawing explanation
Fig. 1 is reactor schematic diagram of the present invention.
Number in the figure is as follows: 1, pump sump; 2, intake pump; 3, aerator; 4, water-in; 5, intake pump controller; 6, aerator controller; 7, stirrer; 8, mechanical stirring machine controller; 9, go out water pump; 10, stirring rake; 11, aeration head; 12, sbr reactor device; 13, water outlet; 14, go out water pump; 15, outlet sump.
Fig. 2 is water outlet PO in Denitrifying Phosphate Accumulating Organisms acclimating process in the present invention
4 3--P concentration schematic diagram.
Number in the figure is as follows: 1, tp removal rate coordinate axis, %; 2, working time coordinate axis, day; 3, water outlet phosphorus concentration coordinate axis, mg/L; 4, tp removal rate; 5, water outlet phosphorus concentration
Embodiment
Test influent quality is as follows:
1～40d，COD=800mg/L，PO
4 3--P=40mg/L；
41～70d，COD=300mg/L，PO
4 3--P=20mg/L；NO
3 --N=30mg/L
When test run, envrionment temperature is 5～10 ℃, and anaerobic stages control dissolved oxygen concentration is less than 0.1mg/L; Aerobic stage control dissolved oxygen concentration is greater than 2.0mg/L; Anoxic stage control redox potential is-100～-40mV.
Concrete processing is as follows:
(1) get sewage work's Aerobic Pond mud and make sequencing batch active sludge reactor seed sludge, sludge concentration is 4200mg/L.
(2) high-load anaerobic/aerobic stage (1～40d): move 3 cycles every day, each cycle, according to water inlet 30min, anaerobic reaction 2h, aerobic reaction 4h, precipitation 1h, draining 30min successively operation automatically, gathers water outlet water sample every day once, measures its PO
4 3--P concentration, and calculate tp removal rate.As shown in Figure 2, through the aerobic/anaerobic operation of 40d, in water outlet, phosphorus concentration is stabilized in below 0.5mg/L.
(3) the underload anaerobic/anoxic stage (41～70d): move 3 cycles every day, each cycle, according to water inlet 30min, anaerobic reaction 2h, hypoxia response 4h, precipitation 1h, draining 30min successively operation automatically, gathers water outlet water sample every day once, measures its PO
4 3--P concentration, and calculate tp removal rate.As shown in Figure 2, change into the initial stage of hypoxia response at aerobic reaction, phosphorus removal effect is poor, but moves through the anaerobic/anoxic of 30d, and in water outlet, phosphorus concentration is stabilized in below 0.5mg/L.Explanation thus, can under low temperature condition, effectively be enriched to Denitrifying Phosphate Accumulating Organisms by " two-part (first aerobic/anaerobic; rear anaerobic/anoxic) acclimating, first high rear low water inlet loads of nutrition, the circular flow pattern of water inlet, reaction, precipitation, draining " this strategy.
Above-described embodiment is only preference of the present invention, is not used for limiting the present invention, all within principle of the present invention, and that does is anyly equal to alternative, modifications and variations, all within protection scope of the present invention.
Claims (2)
1. a Denitrifying Phosphate Accumulating Organisms acclimating method under cold condition, said method comprising the steps of:
(1) design of reactor and building: reactor design adopts sequencing batch active sludge, is mainly made up of water inlet system, aeration agitation system, outlet system and time controlled system.This device can be controlled water inlet, reaction, precipitation, drainage procedure by each controller time series, realizes automatic operating management, greatly alleviated artificial intensity, saved Pollution abatement cost, and device structure is simple, convenient mobile.
(2) aerobic/anaerobic operational mode: by sewage work's Aerobic Pond sludge seeding in this sequencing batch active sludge reactor, acclimating dephosphorization microorganism under aerobic/anaerobic environment, control water inlet in COD concentration be 800mg/L, PO
4 3--P concentration is 40mg/L, gathers its PO of water outlet water determination every day
4 3--P concentration, with PO in water outlet
4 3--P concentration stabilize and reach emission standard and determine that acclimating work completes.
(3) anaerobic/anoxic operational mode: acclimating Denitrifying Phosphate Accumulating Organisms under anaerobic/anoxic environment, controlling COD concentration in water inlet is 300mg/L, PO
4 3--P concentration is 20mg/L, gathers its PO of water outlet water determination every day
4 3--P concentration, with PO in water outlet
4 3--P concentration stabilize and reach emission standard and determine that acclimating work completes.
2. a kind of Denitrifying Phosphate Accumulating Organisms acclimating method under cold condition according to claim 1, is characterized in that: in step (1), flooding time is that 30min, reaction times (anaerobism 2h, aerobic or anoxic 4h), sedimentation time are that 1h, water discharge time are 30min.
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Cited By (8)
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| CN104986858A (en) * | 2015-07-31 | 2015-10-21 | 安徽科技学院 | Synchronous denitrification dephosphorization bacteria enriching and domesticating method |
| CN105236572A (en) * | 2015-10-27 | 2016-01-13 | 深圳德玛克环保科技有限公司 | Method for culturing phosphorus-accumulating particle sludge through low-temperature SBR technology and application |
| CN105692916A (en) * | 2016-02-03 | 2016-06-22 | 浙江裕腾百诺环保科技有限公司 | Reinforcement gathering device and domesticating method for functional bacteria for restoring river channels |
| CN107487838A (en) * | 2017-08-21 | 2017-12-19 | 吉林大学 | SBR tames the method and device that special sludge structure realizes low-temperature sewage efficient dephosphorization |
| CN107585854A (en) * | 2017-09-21 | 2018-01-16 | 北京市自来水集团有限责任公司技术研究院 | The method of BAF, its construction method and the biofilter processing water using this method structure |
| CN111440725A (en) * | 2020-04-17 | 2020-07-24 | 广东省微生物研究所（广东省微生物分析检测中心） | Method for undisturbed enrichment and domestication of functional microorganisms in water sediments |
| CN112939362A (en) * | 2021-02-05 | 2021-06-11 | 四川国润和洁环境科技有限公司 | Method for treating sewage in alpine region |
| CN114702133A (en) * | 2022-03-22 | 2022-07-05 | 北京工业大学 | Method for acclimating denitrifying phosphorus accumulating bacteria by single sludge system |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104986858A (en) * | 2015-07-31 | 2015-10-21 | 安徽科技学院 | Synchronous denitrification dephosphorization bacteria enriching and domesticating method |
| CN105236572B (en) * | 2015-10-27 | 2017-11-24 | 深圳信息职业技术学院 | The method of low temperature SBR process culture polyphosphate particle sludge and application |
| CN105236572A (en) * | 2015-10-27 | 2016-01-13 | 深圳德玛克环保科技有限公司 | Method for culturing phosphorus-accumulating particle sludge through low-temperature SBR technology and application |
| CN105692916B (en) * | 2016-02-03 | 2019-03-08 | 浙江裕腾百诺环保科技有限公司 | A kind of function microbial inoculum reinforcing enriching apparatus and acclimation method for river reparation |
| CN105692916A (en) * | 2016-02-03 | 2016-06-22 | 浙江裕腾百诺环保科技有限公司 | Reinforcement gathering device and domesticating method for functional bacteria for restoring river channels |
| CN107487838A (en) * | 2017-08-21 | 2017-12-19 | 吉林大学 | SBR tames the method and device that special sludge structure realizes low-temperature sewage efficient dephosphorization |
| CN107487838B (en) * | 2017-08-21 | 2020-12-08 | 吉林大学 | Method and device for realizing efficient phosphorus removal of low-temperature sewage by domesticating special sludge structure through SBR (sequencing batch reactor) |
| CN107585854A (en) * | 2017-09-21 | 2018-01-16 | 北京市自来水集团有限责任公司技术研究院 | The method of BAF, its construction method and the biofilter processing water using this method structure |
| CN107585854B (en) * | 2017-09-21 | 2020-05-12 | 北京市自来水集团有限责任公司技术研究院 | Biological aerated filter, construction method thereof and method for treating water by using biological aerated filter constructed by method |
| CN111440725A (en) * | 2020-04-17 | 2020-07-24 | 广东省微生物研究所（广东省微生物分析检测中心） | Method for undisturbed enrichment and domestication of functional microorganisms in water sediments |
| CN111440725B (en) * | 2020-04-17 | 2024-03-01 | 广东省微生物研究所（广东省微生物分析检测中心） | Method for enriching and domesticating functional microorganisms in undisturbed state of water sediment |
| CN112939362A (en) * | 2021-02-05 | 2021-06-11 | 四川国润和洁环境科技有限公司 | Method for treating sewage in alpine region |
| CN114702133A (en) * | 2022-03-22 | 2022-07-05 | 北京工业大学 | Method for acclimating denitrifying phosphorus accumulating bacteria by single sludge system |
| CN114702133B (en) * | 2022-03-22 | 2023-10-10 | 北京工业大学 | Method for domesticating denitrifying phosphorus accumulating bacteria by single sludge system |
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Application publication date: 20140507 |