CN104986858A - Synchronous denitrification dephosphorization bacteria enriching and domesticating method - Google Patents
Synchronous denitrification dephosphorization bacteria enriching and domesticating method Download PDFInfo
- Publication number
- CN104986858A CN104986858A CN201510467975.XA CN201510467975A CN104986858A CN 104986858 A CN104986858 A CN 104986858A CN 201510467975 A CN201510467975 A CN 201510467975A CN 104986858 A CN104986858 A CN 104986858A
- Authority
- CN
- China
- Prior art keywords
- anaerobic
- concentration
- water
- acclimating
- aerobic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention relates to the field of waste water treatment and environmental protection, and discloses a synchronous denitrification dephosphorization bacteria enriching and domesticating method. The method aims to solve the problems that under low temperature environment, synchronous denitrification dephosphorization bacteria are difficult to enrich and domesticate and denitrification and dephosphorization effects for waste water are poor. The method disclosed by the invention adopts a strategy of "two-stage type (firstly anaerobic/aerobic and then anaerobic/anoxic) enriching and domestication, a high-low water inlet nutrition load and a cycle operation mode of water inlet, reaction, precipitation and water drainage", so that the enriching number of dephosphorization microorganisms is increased, the propagation rate of microorganisms is increased, and optimal conditions are created for the propagation of the microorganisms. According to the method disclosed by the invention, through anaerobic/aerobic operation for 40d and anaerobic/anoxic operation for 30d, the synchronous denitrification dephosphorization bacteria are successfully enriched and domesticated under the low temperature environment, and the concentration of phosphorus in effluent water is stabilized to be 0.5mg/L.
Description
Technical field
The present invention relates to sewage disposal and field of environment protection, specifically a kind of denitrification synchronous denitrification dephosphorizing bacterium acclimating method.
Background technology
Nitrogen, phosphoric are two important factors causing body eutrophication, and there is strict restriction in numerous country to its emission concentration.The direct result of body eutrophication accelerates water body ager process exactly, makes water body lose the function of original fishery, tourism and drinking water source.National environmental protection portion of China requires urban wastewater treatment firm water outlet to enter major river valley and lake, reservoir etc. are closed, semiclosed waters time, perform the one-level A standard of " urban wastewater treatment firm pollutant emission standard " (GB18918-2002).Along with the raising of nitrogen, phosphorus emission standard, in newly-built sewage work or old technology upgrading process, the Biological Nitrogen Removal Processe application of high-level efficiency less energy-consumption is particularly important.
At present, Enhanced Biological Phosphorus Removal technique (enhanced biological phosphorus removal, EBPR) is because its efficiency is high, cost is low and environmental friendliness and enjoy focus of attention.Functional microorganism main in this technique is polyP bacteria (Accumulibacter), its principal feature is: under anaerobic poly-phosphorus hydrolysis generate energy (adenosine triphosphate in cell, and reduced coenzyme NADH ATP), for voltaile fatty acid (volatile fatty acid, VFA) absorption also saves as poly-β-hydroxybutyric acid (poly-β-hydroxybutyrate, PHB); Then being electron donor with PHB under aerobic condition, is that electron acceptor(EA) carries out excessive suction phosphorus with oxygen, thus removes the phosphorus in sewage with the form of excess sludge discharge.Along with scholar is to the further investigation of polyP bacteria, find that there is a kind of polyP bacteria can be that electron acceptor(EA) carries out synchronous denitrification denitrogenation dephosphorizing under anoxic conditions with nitrate, is called denitrification synchronous denitrification dephosphorizing bacterium.Denitrification synchronous denitrification dephosphorizing bacterium can save aeration rate 30% compared to aerobic phosphorous accumulating bacterium, reduces mud generation 50%, reduces carbon source demand amount 50% and is particularly suitable for the process of low C/N than sanitary sewage.Be that aerobic phosphorous accumulating bacterium or denitrification synchronous denitrification dephosphorizing bacterium are all very easily subject to having similar metabolic type but do not possess the fusca xylanase competition released phosphorus and inhale phosphorus function in enrichment process, and the factors such as pH, DO, carbon source and temperature will affect the enrichment environment of polyP bacteria and fusca xylanase.In these influence factors, temperature is the major influence factors of polyP bacteria and fusca xylanase enrichment.Chinese scholars has done certain research to the acclimating of denitrification synchronous denitrification dephosphorizing bacterium in normal temperature (25 ± 5 DEG C) situation, achieves some achievements in research and successful experience.
But the acclimating situation of polyP bacteria under research low temperature environment, being applied to practice to EBPR system has important directive significance (especially winter).
Summary of the invention
The object of the present invention is to provide a kind of denitrification synchronous denitrification dephosphorizing bacterium acclimating method.
The object of the present invention is achieved like this:
(1) fill phase: utilize intake pump 2 to pump in reactor 12 by solution in inlet chest 1, controlling flooding time by water inlet controller 5 is 30min;
(2) anaerobic stages: start blast aeration machine 3 and nitrogen is blasted in reactor 12 by aeration head 11, anaerobic environment is provided, passing into the nitrogen time is 2h by aeration controller 6 control; Starting machine mixer 7 drives stirring rake 10 to stir, and provides the power that solution mixes completely, and the mechanical stirring time is controlled to be 2h by mechanical stirring controller 8;
(3) aerobic stage: after anaerobic reaction 2h completes, changes reactor anaerobic environment into aerobic environment, is blasted in reactor 12 by air, provide aerobic environment by blast aeration machine 3 by aeration head 11, passes into air time and is controlled to be 4h by aeration controller 6; Starting machine mixer 7 drives stirring rake 10 to stir, and provides the power that solution mixes completely, and the mechanical stirring time is controlled to be 4h by mechanical stirring controller 8;
(4) precipitate phase: after aerobic reaction 4h completes, aeration controller 6 closes blast aeration machine 3 automatically, and mechanical stirring controller 8 cuts out machine mixer 7 automatically, and sedimentation time is 1h;
(5) bleeding stage: after precipitation 1h completes, starts wet-pit 14 and enters in water tank 15 by precipitation supernatant liquor, and water discharge time is controlled to be 30min by water discharge controller 9;
(6) repeating step (1), step (2), step (3), step (4), step (5), with again complete into water, anaerobism, aerobic, precipitation and drainage procedure, in this process operation 120 cycle, amount to 40d;
(7), after aerobic/anaerobic stage 40d completes, the running status of reactor changes the anaerobic/anoxic stage into;
(8) fill phase: same to step (1) is identical, but influent load reduces;
(9) anaerobic stages: same to step (2) is identical;
(10) anoxic phases: after anaerobic reaction 2h completes, reactor anaerobic environment is changed into anaerobic environment by adding nitrate solution, starting machine mixer 7 drives stirring rake 10 to stir, there is provided the power that solution mixes completely, the mechanical stirring time is controlled to be 4h by mechanical stirring controller 8;
(11) precipitate phase: same to step (4);
(12) bleeding stage: same to step (5)
(13) repeating step (8), step (9), step (10), step (11), step (12), again to complete into water, anaerobism, anoxic, precipitation and drainage procedure, in this process operation 90 cycle, amount to 30d;
(14) taken at regular intervals water outlet water sample, measures PO in water outlet
4 3--P concentration;
In step (1) described inlet chest, solution C OD concentration is 800mg/L, PO
4 3--P concentration is 40mg/L.
The speed that the described blast aeration machine of step (2) passes into nitrogen is 1.5L/min.
The speed that the described blast aeration machine of step (3) passes into air is 1.5L/min.
In step (8) described inlet chest, solution C OD concentration is 300mg/L, PO
4 3--P concentration is 20mg/L.
Nitrate concentration described in step (10) is 30mg/L.
PO described in step (14)
4 3--P method for measurement of concentration is molybdenum-antimony anti-spectrophotometric method.
Main points of the present invention are:
(1) adopt two-part acclimating denitrification synchronous denitrification dephosphorizing bacterium, first acclimating aerobic phosphorous accumulating bacterium under aerobic/anaerobic condition, filters out dephosphorization microorganism; Acclimating denitrification synchronous denitrification dephosphorizing bacterium under anaerobic/anoxic condition, filters out dephosphorization microorganism under anoxic conditions again.
(2) adopt the circular flow pattern of water inlet, reaction, precipitation, draining, reduce nitrate to the impact of anaerobic phosphorus release, and reduce carbon source to the impact of denitrification nitrogen and phosphorus removal.
(3) first high rear low water inlet loads of nutrition is adopted, high at initial operating stage water inlet Middle nutrition load, accelerate dephosphorization microorganism fast enriching at low ambient temperatures; Phase reduces water inlet Middle nutrition load after operation, makes the denitrification synchronous denitrification dephosphorizing bacterium of acclimating adapt to normal sanitary sewage.
Specific embodiment of the invention measure is:
(1) reactor design and build: reactor design adopt sequencing batch active sludge, form primarily of water inlet system, aeration agitation system, outlet system and time controlled system.This device controls water inlet, reaction, precipitation, drainage procedure time series by each controller, realizes automatic operating management, significantly reduces artificial intensity, saves Pollution abatement cost, and device structure is simple, convenient mobile.
(2) aerobic/anaerobic operational mode: by sewage work's Aerobic Pond sludge seeding in this sequencing batch active sludge reactor, acclimating dephosphorization microorganism under aerobic/anaerobic environment, controlling COD concentration in water inlet is 800mg/L, PO
4 3--P concentration is 40mg/L, gathers its PO of water outlet water determination every day
4 3--P concentration, with PO in water outlet
4 3--P concentration stabilize and reach emission standard to determine that acclimating work completes.
(3) anaerobic/anoxic operational mode: acclimating denitrification synchronous denitrification dephosphorizing bacterium under anaerobic/anoxic environment, controlling COD concentration in water inlet is 300mg/L, PO
4 3--P concentration is 20mg/L, gathers its PO of water outlet water determination every day
4 3--P concentration, with PO in water outlet
4 3--P concentration stabilize and reach emission standard to determine that acclimating work completes.
The present invention is applicable to the quick startup of Enhanced Biological Phosphorus Removal technique under low temperature environment, has very strong practical significance and applicability widely.
The present invention compared with domestic and international existing similar technique, its creativeness be following some:
1, have studied the acclimating of denitrification synchronous denitrification dephosphorizing bacterium in Enhanced Biological Phosphorus Removal technique under low temperature environment, for most area sewage disposal in winter qualified discharge provides possibility;
2, adopt first high rear low water inlet loads of nutrition, accelerate the acclimating speed of microorganism;
3, the circular flow pattern of water inlet, reaction, precipitation, draining is adopted, for microbial reproduction creates optimum condition.
The difference of the present invention and domestic and international prior art is:
1, adopt two-part (first aerobic/anaerobic, rear anaerobic/anoxic) acclimating method, enhance the enriched amount of dephosphorization microorganism;
2, adopt first high rear low water inlet loads of nutrition, improve microbial reproduction speed;
3, the circular flow pattern of water inlet, reaction, precipitation, draining is adopted, for microbial reproduction creates optimum condition.
The invention has the beneficial effects as follows:
Adopt two-part (first aerobic/anaerobic, rear anaerobic/anoxic) acclimating method, enhance the enriched amount of dephosphorization microorganism; Adopt first high rear low water inlet loads of nutrition, improve microbial reproduction speed; Adopt the circular flow pattern of water inlet, reaction, precipitation, draining, for microbial reproduction creates optimum condition.By this strategy, the operating performance of Enhanced Biological Phosphorus Removal technique at cold condition can be improved.
Accompanying drawing explanation
Fig. 1 is reactor schematic diagram of the present invention.
Number in the figure is as follows: 1, pump sump; 2, intake pump; 3, aerator; 4, water-in; 5, intake pump controller; 6, aerator controller; 7, stirrer; 8, mechanical stirring machine controller; 9, water pump is gone out; 10, stirring rake; 11, aeration head; 12, sbr reactor device; 13, water outlet; 14, water pump is gone out; 15, outlet sump.
Fig. 2 is water outlet PO in denitrification synchronous denitrification dephosphorizing bacterium acclimating process in the present invention
4 3--P concentration schematic diagram.
Number in the figure is as follows: 1, tp removal rate coordinate axis, %; 2, working time coordinate axis, sky; 3, water outlet phosphorus concentration coordinate axis, mg/L; 4, tp removal rate; 5, water outlet phosphorus concentration
Embodiment
Test influent quality is as follows:
1～40d，COD＝800mg/L，PO
4 3--P＝40mg/L；
41～70d，COD＝300mg/L，PO
4 3--P＝20mg/L；NO
3 --N＝30mg/L
During test run, envrionment temperature is 5 ~ 10 DEG C, and anaerobic stages controls dissolved oxygen concentration and is less than 0.1mg/L; Aerobic stage controls dissolved oxygen concentration and is greater than 2.0mg/L; Anoxic phases controlled oxidization reduction potential is-100 ~-40mV.
Concrete process is as follows:
(1) get sewage work's Aerobic Pond mud and make sequencing batch active sludge reactor seed sludge, sludge concentration is 4200mg/L.
(2) high-load anaerobic/aerobic stage (1 ~ 40d): run 3 cycles every day, each cycle is run successively automatically according to water inlet 30min, anaerobic reaction 2h, aerobic reaction 4h, precipitation 1h, draining 30min, gather water outlet water sample every day once, measure its PO
4 3--P concentration, and calculate tp removal rate.As shown in Figure 2, the aerobic/anaerobic through 40d runs, and in water outlet, phosphorus concentration is stabilized in below 0.5mg/L.
(3) the underload anaerobic/anoxic stage (41 ~ 70d): run 3 cycles every day, each cycle is run successively automatically according to water inlet 30min, anaerobic reaction 2h, hypoxia response 4h, precipitation 1h, draining 30min, gather water outlet water sample every day once, measure its PO
4 3--P concentration, and calculate tp removal rate.As shown in Figure 2, change into the initial stage of hypoxia response at aerobic reaction, phosphorus ligands effect is poor, but runs through the anaerobic/anoxic of 30d, and in water outlet, phosphorus concentration is stabilized in below 0.5mg/L.Illustrate thus, denitrification synchronous denitrification dephosphorizing bacterium can be effectively enriched at low temperatures by " two-part (first aerobic/anaerobic; rear anaerobic/anoxic) acclimating, first high rear low water inlet loads of nutrition, the circular flow pattern of water inlet, reaction, precipitation, draining " this strategy.
Above-described embodiment is only preference of the present invention, is not used for limiting the present invention, all within principle of the present invention, and any equivalent alternative, the modifications and variations done, all within protection scope of the present invention.
Claims (2)
1. a denitrification synchronous denitrification dephosphorizing bacterium acclimating method, said method comprising the steps of:
(1) reactor design and build: reactor design adopt sequencing batch active sludge, form primarily of water inlet system, aeration agitation system, outlet system and time controlled system.This device controls water inlet, reaction, precipitation, drainage procedure time series by each controller, realizes automatic operating management, significantly reduces artificial intensity, saves Pollution abatement cost, and device structure is simple, convenient mobile.
(2) aerobic/anaerobic operational mode: by sewage work's Aerobic Pond sludge seeding in this sequencing batch active sludge reactor, acclimating dephosphorization microorganism under aerobic/anaerobic environment, controlling COD concentration in water inlet is 800mg/L, PO
4 3--P concentration is 40mg/L, gathers its PO of water outlet water determination every day
4 3--P concentration, with PO in water outlet
4 3--P concentration stabilize and reach emission standard to determine that acclimating work completes.
(3) anaerobic/anoxic operational mode: acclimating Denitrifying Phosphate Accumulating Organisms under anaerobic/anoxic environment, controlling COD concentration in water inlet is 300mg/L, PO
4 3--P concentration is 20mg/L, gathers its PO of water outlet water determination every day
4 3--P concentration, with PO in water outlet
4 3--P concentration stabilize and reach emission standard to determine that acclimating work completes.
2. a kind of denitrification synchronous denitrification dephosphorizing bacterium acclimating method according to claim 1, is characterized in that: in step (1), flooding time is 30min, reaction times (anaerobism 2h, aerobic or anoxic 4h), sedimentation time be 1h, water discharge time is 30min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510467975.XA CN104986858A (en) | 2015-07-31 | 2015-07-31 | Synchronous denitrification dephosphorization bacteria enriching and domesticating method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510467975.XA CN104986858A (en) | 2015-07-31 | 2015-07-31 | Synchronous denitrification dephosphorization bacteria enriching and domesticating method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104986858A true CN104986858A (en) | 2015-10-21 |
Family
ID=54298785
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510467975.XA Pending CN104986858A (en) | 2015-07-31 | 2015-07-31 | Synchronous denitrification dephosphorization bacteria enriching and domesticating method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104986858A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105236572A (en) * | 2015-10-27 | 2016-01-13 | 深圳德玛克环保科技有限公司 | Method for culturing phosphorus-accumulating particle sludge through low-temperature SBR technology and application |
| CN114790069A (en) * | 2022-04-14 | 2022-07-26 | 北控技术服务(广东)有限公司 | Biological phosphorus removal effect evaluation method for municipal sewage treatment plant |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7172699B1 (en) * | 2004-10-13 | 2007-02-06 | Eimco Water Technologies Llc | Energy efficient wastewater treatment for nitrogen and phosphorus removal |
| CN103773726A (en) * | 2014-02-09 | 2014-05-07 | 安徽科技学院 | Method for enriching and acclimating denitrifying phosphate-accumulating organisms at low temperature |
-
2015
- 2015-07-31 CN CN201510467975.XA patent/CN104986858A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7172699B1 (en) * | 2004-10-13 | 2007-02-06 | Eimco Water Technologies Llc | Energy efficient wastewater treatment for nitrogen and phosphorus removal |
| CN103773726A (en) * | 2014-02-09 | 2014-05-07 | 安徽科技学院 | Method for enriching and acclimating denitrifying phosphate-accumulating organisms at low temperature |
Non-Patent Citations (1)
| Title |
|---|
| 胡亮等: "分点进水高效除磷脱氮工艺工程应用研究", 《环境科技》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105236572A (en) * | 2015-10-27 | 2016-01-13 | 深圳德玛克环保科技有限公司 | Method for culturing phosphorus-accumulating particle sludge through low-temperature SBR technology and application |
| CN105236572B (en) * | 2015-10-27 | 2017-11-24 | 深圳信息职业技术学院 | The method of low temperature SBR process culture polyphosphate particle sludge and application |
| CN114790069A (en) * | 2022-04-14 | 2022-07-26 | 北控技术服务(广东)有限公司 | Biological phosphorus removal effect evaluation method for municipal sewage treatment plant |
| CN114790069B (en) * | 2022-04-14 | 2023-12-22 | 北控技术服务(广东)有限公司 | Biological dephosphorization effect evaluation method for municipal wastewater treatment plant |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103773726A (en) | Method for enriching and acclimating denitrifying phosphate-accumulating organisms at low temperature | |
| CN103880181B (en) | The improvement A of a kind of Continuous Flow short-cut nitrification and denitrification dephosphorization 2/ O technique | |
| CN101628772B (en) | Short distance denitrifying dephosphatation double-sludge technique taking granular sludge as medium and device thereof | |
| CN104193003B (en) | The low CN of aerobic/anaerobic SNDPR system process of enrichment polyP bacteria is than the technique of sanitary sewage | |
| CN100569669C (en) | Anaerobic-anoxic oxidation pitch technique denitrification dephosphorization device and method thereof | |
| CN105110572B (en) | A kind of apparatus and method of carbon source absorption/nitrification/part denitrification/low C/N municipal sewages of Anammox coupling technique processing | |
| CN101805094B (en) | Method for starting single-stage self-supported denitrification reactor | |
| CN103708682B (en) | Method for coupling denitrifying dephosphatation and partial nitrification | |
| CN103663681B (en) | Device and method for utilizing carbon source in sludge to treat urban sewage through in-depth nitrogen and phosphorus removal by SBR (sequencing batch reactor) | |
| CN101372378B (en) | Method for using SBR for quick start of continuous flow double sludge denitrification dephosphorization process | |
| CN105540846A (en) | Low-carbon-source municipal sewage improved UCT autotrophic denitrification dephosphorization device and application method thereof | |
| CN103833134B (en) | Method for achieving short-cut denitrification by continuous flow sewage treatment system | |
| CN102583745B (en) | Improved in-situ excess sludge decrement control method through cyclic activated sludge technology | |
| CN104609557A (en) | Method for treating slaughter wastewater by using sequencing batch reactor (SBR) activated sludge process | |
| CN105541008B (en) | The low C/N of breeding wastewater is than anaerobism biogas slurry efficient denitrification carbon removal treatment technique | |
| CN110002591B (en) | Device and method for realizing shortcut nitrification-denitrification dephosphorization coupling of municipal domestic sewage under anoxic/aerobic alternate condition | |
| CN108658229B (en) | Device and method for controlling autotrophic/heterotrophic deep denitrification process | |
| CN104058551A (en) | Energy-saving high-efficiency municipal sewage autotrophic denitrification biological treatment method and device | |
| CN104370422A (en) | Device and method for coupling short-cut denitrification phosphorus removal with anaerobic ammonia oxidation | |
| CN113233592B (en) | Treatment device and method for realizing synchronous deep denitrification and decarbonization of advanced landfill leachate and domestic sewage | |
| CN104944582A (en) | Test device and method of coupled SBR denitrification dephosphorization and integrated anaerobic ammonia oxidation | |
| CN101186387A (en) | Method for increasing organism synchronous dephosphorization denitrogenation effect of sewage under anaerobic-hypoxia condition | |
| CN116855437A (en) | Method for rapidly culturing fermentation type denitrification phosphorus accumulating bacteria by utilizing nitrite | |
| CN104773926A (en) | High-nitrogen-content organic wastewater deep denitrification device and deep denitrification method | |
| CN102583897B (en) | Device and method for strengthening A2/O denitrification |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20151021 |
|
| WD01 | Invention patent application deemed withdrawn after publication |