CN101948177B - Method for enriching denitrifying phosphorus removal bacteria in continuous flow reactor - Google Patents
Method for enriching denitrifying phosphorus removal bacteria in continuous flow reactor Download PDFInfo
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Abstract
The invention discloses a method for enriching denitrifying phosphorus removal bacteria in a continuous flow reactor (CFR). In the method of the invention, phosphorus accumulating bacteria and denitrifying phosphorus removal bacteria are sequentially enriched in the CFR, wherein the phosphorus accumulating bacteria enrichment is carried out in an anaerobic / aerobic (AO) mode, and the denitrifying phosphorus removal bacteria enrichment is carried out in an anaerobic / anoxic / aerobic (AAO) mode. In the method, when denitrifying phosphorus removal bacteria are enriched, a fast aeration zone is added on the basis of the original anaerobic / anoxic (AA) mode to form the anaerobic / anoxic / aerobic (AAO) mode, thus ensuring the activity of the denitrifying phosphorus bacteria in the system. Compared withthe method using a batch reactor (SBR), the method of the invention has the advantage of simple and convenient operation, and is applied more widely in practice.
Description
Technical field
The invention belongs to the denitrification dephosphorization technical field, relate in particular to a kind of method of gathering denitrifying polyP bacteria.
Background technology
The body eutrophication that nitrogen, phosphorus excessive emissions cause is one of environmental problem of comparatively paying close attention to of the current public, and the denitrogenation dephosphorizing that turns to purpose with the control water eutrophication has become the major objective of whole world sewage treatment area.The denitrification dephosphorization technology is the state-of-the-art technology of present sewage denitrification and dephosphorization, and this technology has successfully solved the carbon source contention contradiction in traditional denitrification dephosphorization technique, has reached the effect of " carbon is dual-purpose ", meets the sustainable development idea of China.
Denitrification phosphorus-collecting bacterium (Denitrifying Phosphorus-removal Bacteria is abbreviated as DPB) is the special bacterium that a class is used to the denitrification dephosphorization technology, and this bacterial classification is with O
2And NO
3 --N under anaerobic decomposes intravital poly-phosphorus as electron acceptor(EA), and absorbs easy organism of degrading and poly-with PHB(in the sewage
β-hydroxybutyric acid) form stores in vivo; Under aerobic or anoxia condition, decompose intravital PHB, and with NO
3 --N is that electron acceptor(EA) is with in the phosphorus suction body in the sewage.
The denitrification phosphorus-collecting bacterium is used for the denitrification dephosphorization technology, and the matter of utmost importance that solve is the enrichment culture of denitrification phosphorus-collecting bacterium.The denitrification phosphorus-collecting bacterium is a kind of of polyP bacteria, generally adopts two-stage method to cultivate: earlier former active sludge is passed through aerobic/anaerobic (AO) pattern enrichment polyP bacteria, then by anaerobic/anoxic (AA) pattern gathering denitrifying dephosphorization bacterial.
The Chinese patent publication number is CN 101372378A, open day was that February 25, denomination of invention in 2009 are that SBR is used for the continuous flow double sludge denitrification dephosphorization process patent of the method for startup fast, disclose and adopted two-stage method to cultivate the technical scheme of denitrification phosphorus-collecting bacterium, and obtained effect preferably, but lack the aeration activation microorganism stage in this patented method when cultivating the denitrification phosphorus-collecting bacterium, microorganism active is difficult to be guaranteed in the system; In addition, the batch reactor (SBR) that this patent adopts, though have the advantage of flexible control, level of automation is higher, operation is inconvenience comparatively; And domestic and international existing Sewage treatment systems mainly is to be based upon on the basis of continuous flow reactor (CFR) at present, so the application in practice of this patented method also is restricted.
Summary of the invention
At the deficiencies in the prior art, the present invention is a purpose with denitrification phosphorus-collecting bacterium activity in the raising system, provide a kind of easy and simple to handle in continuous flow reactor (CFR) method of gathering denitrifying polyP bacteria.
For solving the problems of the technologies described above, the present invention adopts following technical scheme:
A kind of in continuous flow reactor the method for gathering denitrifying polyP bacteria, may further comprise the steps:
1) seed sludge is transferred to continuous flow reactor;
2) enrichment polyP bacteria: take aerobic/anaerobic pattern enrichment polyP bacteria, only to the anaerobic zone water inlet, when the anaerobic phosphorus release amount of mud or aerobic suction phosphorus amount are 2 ~ 4mg/L, change the two-stage water mode of doing, when initial simultaneously to anaerobic zone and aerobic zone water inlet;
3) gathering denitrifying polyP bacteria: behind the enrichment polyP bacteria, in the anaerobic zone and the middle oxygen-starved area of introducing of aerobic zone of continuous flow reactor, simultaneously to anaerobic zone and oxygen-starved area water inlet, gathering denitrifying polyP bacteria under anaerobic/anoxic/aerobic pattern.
In the above-mentioned enrichment polyP bacteria process, phosphate concn was 5 ~ 10mg/L during the maintenance anaerobic zone was intake when initial, change make the two-stage water mode after, phosphate concn is respectively 2.5 ~ 2.8L/h and 3 ~ 5mg/L in keeping the anaerobic zone flooding velocity and intaking, phosphate concn is respectively 0.3 ~ 0.5L/h and 50 ~ 60mg/L in aerobic zone flooding velocity and the water inlet, the every operating parameter of in this process other is: anaerobic zone and aerobic zone water inlet COD value all are controlled at 350 ~ 400mg/L, the anaerobic zone ammonia nitrogen concentration is 8 ~ 10mg/L, aerobic zone DO is 2 ~ 4mg/L, the anaerobism residence time is 3 ~ 4h, the aerobic residence time is 2 ~ 3h, sludge concentration maintains 2800 ~ 3000 mg/L, and return sludge ratio maintains between 100 ~ 200%, and sludge age (SRT) was controlled at 10 ~ 13, the pH value is 7 ~ 8, and temperature is 20 ~ 25 ℃.
In the above-mentioned gathering denitrifying polyP bacteria process, phosphate concn is 8 ~ 10mg/L in the water inlet of maintenance anaerobic zone, NO in the water inlet of oxygen-starved area
3 --N concentration is 10 ~ 20mg/L; When taking place to inhale the phosphorus phenomenon, further improve NO in the water inlet of oxygen-starved area
3 --N concentration is 25 ~ 30mg/L, the every operating parameter of in this process other is: oxygen-starved area water inlet COD value is controlled at 300 ~ 350mg/L, the anaerobism residence time is 2 ~ 2.5h, the anoxic residence time is 3.8 ~ 4h, and the aerobic residence time is 0.8 ~ 1h, and sludge concentration maintains 2500 ~ 2800 mg/L, return sludge ratio maintains between 50 ~ 100%, sludge age (SRT) was controlled at 13 ~ 15, and the pH value is 7 ~ 8, and temperature is 20 ~ 25 ℃.
Described anaerobic zone and aerobic zone water inlet COD value are provided by the composition of sodium acetate, propionic acid or sodium acetate and propionic acid.
Compared with prior art, the present invention has the following advantages and beneficial effect:
1, the enriching method of denitrification phosphorus-collecting bacterium is to have increased a quick aeration zone on original anaerobic/anoxic (AA) pattern basis among the present invention, constitute anaerobic/anoxic/aerobic (AAO) pattern, further reduce residual COD and phosphoric acid salt, be beneficial to the phosphorus of releasing of anaerobic zone, guaranteed the activity of denitrification phosphorus-collecting bacterium in the system;
2, the present invention adopts continuous flow reactor (CFR) to replace batch reactor (SBR) enrichment culture denitrification phosphorus-collecting bacterium, and the space continuity replacement time continuity with reactor can carry out the enrichment process of denitrification phosphorus-collecting bacterium continuously, and easy and simple to handle;
3, the present invention has replenished the training method of denitrification phosphorus-collecting bacterium, the Sewage treatment systems of domestic and international application mainly is to be based upon on the basis of continuous flow reactor (CFR) at present, with respect to gathering denitrifying polyP bacteria in batch reactor (SBR), the application in practice of the method for gathering denitrifying polyP bacteria is more extensive in continuous flow reactor.
Description of drawings
Fig. 1 is the inventive method equipment therefor figure;
Fig. 2 is the variation of poly-phosphorus sludge domestication meta-phosphate concentration;
Fig. 3 is the contrast that the 1st day mud of domestication is inhaled the phosphorus amount under the AAO pattern under aerobic and anoxia condition;
Fig. 4 is the contrast that the 11st day mud of domestication is inhaled the phosphorus amount under the AAO pattern under aerobic and anoxia condition.
Among the figure: water tank 1, peristaltic pump 2, agitator 3, peristaltic pump 4, water tank 5, oxygen increasing pump 6, settling tank 7, aeration head 8, continuous flow reactor 9, reaction zone 10, reaction zone 11, reaction zone 12, reaction zone 13.
Embodiment
The present invention is aerobic/anaerobic (AO) and anaerobic/anoxic/aerobic (AAO) the two kinds of patterns successively moved in homemade continuous flow reactor, and single water intake mode combined with the two-stage water mode, adopt aerobic zone to add the water intake mode that high concentration phosphorus hydrochlorate and oxygen-starved area add high density nitrate, set up the polyP bacteria of high selectivity and the culture environment of denitrification phosphorus-collecting bacterium, thereby be implemented in the enrichment of denitrification phosphorus-collecting bacterium in the continuous flow reactor.
Below in conjunction with drawings and Examples technical scheme of the present invention is described further.
Fig. 1 is the inventive method equipment therefor, and this device main body partly is a continuous flow reactor 9, successively can realize two kinds of operational modes in this reactor: 1) aerobic/anaerobic (AO) pattern is used for enhanced biological phosphorus removal system and enrichment polyP bacteria; 2) anaerobic/anoxic/aerobic (AAO) pattern is used for the gathering denitrifying polyP bacteria.When operation AO pattern, reaction zone 10 and reaction zone 11 are anaerobic zone, and reaction zone 12 and reaction zone 13 are aerobic zone; When operation AAO pattern, reaction zone 10 is an anaerobic zone, and reaction zone 11 and reaction zone 12 are the oxygen-starved area, and reaction zone 13 is quick aeration zone.
The specific embodiment of the present invention is as follows:
1) seed sludge is transferred to continuous flow reactor:
Earlier seed sludge is carried out pre-treatment, comprise the vexed sewage elutriation of exposing to the sun, use human configuration, then pretreated mud is transferred in the continuous flow reactor 9; The useful volume of continuous flow reactor of the present invention is 20.7L, is former water between the sludge acclimatization incubation period with the sewage of artificial preparation.
2) adopt aerobic/anaerobic (AO) pattern to come the enrichment polyP bacteria:
This process is divided into two stages, and the purpose of fs is to allow the polyP bacteria that contains some amount in the seed sludge as early as possible, and the purpose of subordinate phase is to strengthen the enrichment polyP bacteria.
Fs is to adopt single water intake mode, promptly will intake with peristaltic pump 2 and pump into anaerobic zone from water tank 1, and phosphate concn was 5 ~ 10 mg/L during maintenance was intake, and when the anaerobic phosphorus release amount of mud or aerobic suction phosphorus amount are 2 ~ 4mg/L, enters subordinate phase;
Subordinate phase is changed the two-stage water mode of doing, promptly will intake when pumping into anaerobic zone from water tank 1 with peristaltic pump 2, to intake with peristaltic pump 4 and to pump into aerobic zone from water tank 5, in guaranteeing the anaerobic zone water inlet, has the phosphatic while of finite concentration, aerobic zone top Continuous Flow is added the high concentration phosphorus hydrochlorate, phosphate concn is distributed as 2.5 ~ 2.8L/h and 3 ~ 5mg/L in anaerobic zone flooding velocity and the water inlet, and phosphate concn is respectively 0.3 ~ 0.5L/h and 50 ~ 60mg/L in aerobic zone flooding velocity and the water inlet.
The stirring that during the enrichment polyP bacteria, keeps agitator, other parameters during this period are: anaerobic zone and aerobic zone water inlet COD value all are controlled at 350 ~ 400mg/L, the anaerobic zone ammonia nitrogen concentration is 8 ~ 10mg/L, aerobic zone DO is 2 ~ 4mg/L, the anaerobism residence time is 3 ~ 4h, the aerobic residence time is 2 ~ 3h, sludge concentration maintains 2800 ~ 3000 mg/L, return sludge ratio maintains between 100 ~ 200%, and sludge age (SRT) was controlled at 10 ~ 13, and the pH value is 7 ~ 8, temperature is 20 ~ 25 ℃, wherein, anaerobic zone and aerobic zone water inlet COD value respectively provide half by sodium acetate and propionic acid, and the anaerobic zone ammonia nitrogen concentration is provided by ammonium chloride.
When the dephosphorizing rate of sludge system surpasses 80%, illustrate that the polyP bacteria in the mud has become dominant microflora, finished the enrichment of polyP bacteria.Fig. 2 is the 11st, 15,20 day poly-phosphorus sludge domestication effect of domestication, and the dephosphorizing rate of mud is 82.6% when taming the 20th day, has finished the enrichment of polyP bacteria.
3) adopt anaerobic/anoxic/aerobic (AAO) pattern to come the gathering denitrifying polyP bacteria:
Behind the enrichment polyP bacteria, anaerobic zone and the middle oxygen-starved area of introducing of aerobic zone at continuous flow reactor, to intake when pumping into anaerobic zone from water tank 1 with peristaltic pump 2, will intake with peristaltic pump 4 pumps into the oxygen-starved area from water tank 5, keeps that phosphate concn is 8 ~ 10mg/L in the anaerobic zone water inlet.In order to prevent too much residual NO
3 --N enters anaerobic zone, and then causes that common denitrifying bacteria utilizes carbon source to carry out the denitrification activity, and anaerobic phosphorus release is produced restraining effect, NO in the water inlet of oxygen-starved area
3 --N concentration is controlled at 10 ~ 20mg/L in earlier stage, when taking place to inhale the phosphorus phenomenon, further improves NO in the water inlet of oxygen-starved area
3 --N concentration is 25 ~ 30mg/L, NO in the water inlet of oxygen-starved area
3 -The control of-N concentration can be by residual NO in control oxygen-starved area water outlet and the returned sluge
3 --N concentration realizes.
Agitator keeps stirring during the gathering denitrifying polyP bacteria, other parameters during this period are: oxygen-starved area water inlet COD value is controlled at 300 ~ 350mg/L, the anaerobism residence time is 2 ~ 2.5h, the anoxic residence time is 3.8 ~ 4h, and the aerobic residence time is 0.8 ~ 1h, and sludge concentration maintains 2500 ~ 2800 mg/L, return sludge ratio maintains between 50 ~ 100%, sludge age (SRT) was controlled at 13 ~ 15, and the pH value is 7 ~ 8, and temperature is 20 ~ 25 ℃.
During the enrichment of denitrification phosphorus-collecting bacterium, the quantity of denitrification phosphorus-collecting bacterium in the mud is carried out periodic evaluation.The method of estimation that the present invention adopts is: the anoxic that draws mud according to the suction phosphorus situation of mud under anoxia condition and aerobic condition is inhaled phosphorus speed
With aerobic suction phosphorus speed
, the time of wherein calculating suction phosphorus speed is for being fixed as 90min, then by formula
Calculate denitrification phosphorus-collecting bacterium shared ratio in whole polyP bacterias
Fig. 3 inhales the contrast of phosphorus amount at the 1st day mud of domestication under the AAO pattern under aerobic and anoxia condition, Fig. 4 inhales the contrast of phosphorus amount at the 11st day mud of domestication under the AAO pattern under aerobic and anoxia condition, go out the ratio that denitrification phosphorus-collecting bacterium in the continuous flow reactor system accounts for whole polyP bacterias according to the data estimation of Fig. 3 and Fig. 4 and be respectively 28.1% and 51.4%, reached the requirement of denitrification phosphorus-collecting bacterium fast enriching.
Claims (4)
1. the method for a gathering denitrifying polyP bacteria in continuous flow reactor is characterized in that, may further comprise the steps:
1) seed sludge is transferred to continuous flow reactor;
2) enrichment polyP bacteria: take aerobic/anaerobic pattern enrichment polyP bacteria, only to the anaerobic zone water inlet, when the anaerobic phosphorus release amount of mud or aerobic suction phosphorus amount are 2~4mg/L, change the two-stage water mode of doing, when initial simultaneously to anaerobic zone and aerobic zone water inlet;
3) gathering denitrifying polyP bacteria: behind the enrichment polyP bacteria, in the anaerobic zone and the middle oxygen-starved area of introducing of aerobic zone of continuous flow reactor, simultaneously to anaerobic zone and oxygen-starved area water inlet, gathering denitrifying polyP bacteria under anaerobic/anoxic/aerobic pattern.
2. according to claim 1 in continuous flow reactor the method for gathering denitrifying polyP bacteria, it is characterized in that: in the described enrichment polyP bacteria process, phosphate concn was 5~10mg/L during the maintenance anaerobic zone was intake when initial, change make the two-stage water mode after, phosphate concn is respectively 2.5~2.8L/h and 3~5mg/L in keeping the anaerobic zone flooding velocity and intaking, phosphate concn is respectively 0.3~0.5L/h and 50~60mg/L in aerobic zone flooding velocity and the water inlet, the every operating parameter of in this process other is: anaerobic zone and aerobic zone water inlet COD value all are controlled at 350~400mg/L, the anaerobic zone ammonia nitrogen concentration is 8~10mg/L, aerobic zone DO is 2~4mg/L, the anaerobism residence time is 3~4h, the aerobic residence time is 2~3h, sludge concentration maintains 2800~3000mg/L, return sludge ratio maintains between 100~200%, sludge age was controlled at 10~13, and the pH value is 7~8, and temperature is 20~25 ℃.
3. according to claim 1 in continuous flow reactor the method for gathering denitrifying polyP bacteria, it is characterized in that: in the described gathering denitrifying polyP bacteria process, keep that phosphate concn is 8~10mg/L in the anaerobic zone water inlet, NO in the water inlet of oxygen-starved area
3 --N concentration is 10~20mg/L; When taking place to inhale the phosphorus phenomenon, further improve NO in the water inlet of oxygen-starved area
3 --N concentration is 25~30mg/L, the every operating parameter of in this process other is: oxygen-starved area water inlet COD value is controlled at 300~350mg/L, the anaerobism residence time is 2~2.5h, the anoxic residence time is 3.8~4h, and the aerobic residence time is 0.8~1h, and sludge concentration maintains 2500~2800mg/L, return sludge ratio maintains between 50~100%, sludge age was controlled at 13~15, and the pH value is 7~8, and temperature is 20~25 ℃.
4. according to claim 2 in continuous flow reactor the method for gathering denitrifying polyP bacteria, it is characterized in that: described anaerobic zone and aerobic zone water inlet COD value are provided by the composition of sodium acetate, propionic acid or sodium acetate and propionic acid.
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CN102864098B (en) * | 2012-04-26 | 2014-01-01 | 哈尔滨工业大学宜兴环保研究院 | Denitrification phosphorus removal bacterium H-hrb02 as well as screening method and application thereof |
CN103241915B (en) * | 2013-05-22 | 2014-08-27 | 东北林业大学 | Rapid preparation method of denitrification phosphorus-accumulating bacteria granule sludge |
CN103880181B (en) * | 2014-03-10 | 2015-08-05 | 北京工业大学 | The improvement A of a kind of Continuous Flow short-cut nitrification and denitrification dephosphorization 2/ O technique |
CN106390938A (en) * | 2016-09-30 | 2017-02-15 | 东莞市联洲知识产权运营管理有限公司 | Manganese-dioxide-modified sludge activated carbon with abundant phosphorus accumulating bacteria and method for preparing manganese-dioxide-modified sludge activated carbon |
CN109231477B (en) * | 2018-09-04 | 2021-11-26 | 江苏孚璋生物技术有限公司 | Control method for stable operation of all-biological phosphorus removal AOO process under normal temperature working condition and normal-temperature to low-temperature working condition |
CN109231478B (en) * | 2018-09-04 | 2022-04-01 | 江苏孚璋生物技术有限公司 | Starting method of all-biological phosphorus removal AOO process |
CN109231479B (en) * | 2018-09-04 | 2022-04-01 | 江苏孚璋生物技术有限公司 | Control method for stable operation of all-biological phosphorus removal AOO process under low-temperature and ultralow-temperature working conditions and working conditions from low temperature to normal temperature |
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周康群等.反硝化聚磷菌的富集及富集污泥活性研究.《环境科学与技术》.2008,第31卷(第4期), * |
李勇智等.强化生物除磷体系中反硝化聚磷菌的选择与富集.《环境科学学报》.2004,第24卷(第1期), * |
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