CN103708616A - Method for culturing nitrosation granular sludge by matching with anaerobic ammonia oxidation - Google Patents
Method for culturing nitrosation granular sludge by matching with anaerobic ammonia oxidation Download PDFInfo
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- CN103708616A CN103708616A CN201410000669.0A CN201410000669A CN103708616A CN 103708616 A CN103708616 A CN 103708616A CN 201410000669 A CN201410000669 A CN 201410000669A CN 103708616 A CN103708616 A CN 103708616A
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- sludge
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- nitrosated granular
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Abstract
The invention discloses a method for culturing nitrosation granular sludge by matching with anaerobic ammonia oxidation, and belongs to the technical field of biological treatment of wastewater. Flocculent activated sludge rich in ammonia oxidizing bacteria is inoculated by adopting a sequencing batch activated sludge reactor, the nitrosation granular sludge can be cultured within 90-120 days through controlling parameters such as inflow water quality, surface raising airflow, dissolved oxygen, temperature, hydraulic retention time and sedimentation time of the reactor, and the effluent quality of the nitrosation granular sludge reactor meets the inflow requirement of anaerobic ammonia oxidation. According to the method, through realizing the granulation of the nitrosation sludge, the sludge has excellent sedimentation performance, the sludge and water separation property of a system can be effectively enhanced, and the granular sludge has the advantages of difficulty in causing sludge bulking, strong toxin impaction resistance capacity and high ammonia nitrogen loading capacity. Meanwhile, the granular sludge is higher in microbial biomass, high in reaction rate during treatment of high ammonia-nitrogen wastewater, and capable of reducing the volume of the reactor, the hydraulic retention time, the volume of a secondary sedimentation tank, and the like.
Description
Technical field
The invention belongs to biological wastewater treatment technology field, be specifically related to the cultural method a kind of and nitrosated granular sludge that Anammox matches.
Background technology
Anammox technology (Anammox) is a novel energy-saving biological denitride technology of Dutch Delft university Developing in 20 end of the century.Under Anammox bacterium exists, with NH
4 +-N is electron donor, NO
2 --N is that electron acceptor(EA), azanol and hydrazine are key intermediate species and N
2for final product, have power consumption low, without oxygen participate in, without carbon source and the advantage such as sludge yield is low, and be successfully applied to the improvement of the high ammonia-nitrogen wastewaters such as coking chemical waste water, percolate, caused the extensive concern of whole world researcher.
Because Anammox technique needs NO
2 --N is as electron acceptor(EA), and therefore, Anammox technique generally needs preposition Short-Cut Nitrification Process by a part of NH in waste water
4 +-N changes into NO
2 --N.The object of Short-Cut Nitrification Process is that approximately 50% ammonia nitrogen in reactor is oxidized to NO
2 --N the stage, and stop it further to generate NO
3 --N, carries out denitrification in the Anammox technique by follow-up.The key of Short-Cut Nitrification Process is the enrichment of ammonia oxidation bacteria (AOB) and eliminating of NOB (NOB).At present, controlling reactor in lower dissolved oxygen concentration, utilize AOB different to the avidity of dissolved oxygen with NOB, is more common method thereby optionally suppress NOB.But under low DO condition, the nitrification activity of nitrosation reactor is lower, cause speed of reaction to decline, and the environment of low dissolved axygen easily causes thread fungus to expand and sludge settling property decline, these drawbacks limit the further promotion and application of nitrosification-Anammox coupling technique.
The object of the present invention is to provide the cultural method a kind of and nitrosated granular sludge that Anammox matches, by sequencing batch activated sludge reactor (SBR) inoculation, be rich in the cotton-shaped active sludge of ammonia oxidation bacteria (AOB), control the parameters such as influent quality, aeration rate, dissolved oxygen and hydraulic detention time of reactor, take the settling time as selecting pressure, can in 90 ~ 120 days, turn out nitrosated granular sludge, the effluent quality of nitrosated granular sludge reactor meets the water inlet requirement of Anammox.Meanwhile, nitrosated granular sludge reactor organisms amount is large, operational efficiency is high, sludge settling property good, than traditional nitrosification activated sludge reactor, has obvious advantage.
Summary of the invention
The cultural method that the object of this invention is to provide the nitrosated granular sludge that a kind of and Anammox match, solves that existing nitrosification activated sludge process operational efficiency is low, the poor difficult problem of sludge settling property.In order to achieve the above object, the present invention is by the following technical solutions:
And a cultural method for the nitrosated granular sludge that Anammox matches, is characterized in that comprising the steps:
(1) adopt sequencing batch activated sludge reactor (SBR) domestication to cultivate nitrosated granular sludge, the cotton-shaped active sludge of ammonia oxidation bacteria is rich in reactor inoculation, and seed sludge volume accounts for 30 ~ 50% of reactor cumulative volume;
(2) configuration sbr reactor device water inlet, pH is 7.5 ~ 8.0, inlet COD concentration is 400 ~ 800 mg/L, water inlet NH
4 +-N concentration is 600 ~ 800 mg/L, and water inlet total phosphorus concentration is 50 ~ 80 mg/L;
(3) control surface rising tolerance 1.0 ~ 2.5 m/min of sbr reactor device, dissolved oxygen is 1 ~ 3 mg/L, temperature is 25 ~ 30 ℃, hydraulic detention time 8 ~ 16 h, settling times 2 ~ 15 min, can in 90 ~ 120 days, turn out nitrosated granular sludge, the effluent quality of nitrosated granular sludge reactor meets the water inlet requirement of Anammox.
The cultural method of the nitrosated granular sludge that described a kind of and Anammox matches, it is characterized in that: in described step (1), sbr reactor device aspect ratio is 5 ~ 10, reactor adopts bottom water inlet and bottom micro-pore aeration, and the discharge rate of controlling reactor is 50%.
The cultural method of the nitrosated granular sludge that described a kind of and Anammox matches, it is characterized in that: the nitrosated granular sludge color in described step (3) is tawny, be shaped as spherical or elliposoidal, median size is 0.8 ~ 3.0mm, and settling velocity is 20 ~ 50 m/h.
The cultural method of the nitrosated granular sludge that described a kind of and Anammox matches, is characterized in that: the effluent quality of the nitrosated granular sludge reactor in described step (3) is NH
4 +-N and NO
2 --N concentration ratio is 1:(1 ~ 1.5), NO
3 --N concentration 30 ~ 60 mg/L, pH value is 7.7 ~ 8.5.
The cultural method of the nitrosated granular sludge that a kind of and Anammox provided by the invention match, advantage and the feature of its embodiment are:
(1) than traditional nitrosification active sludge, nitrosated granular sludge has good settling property, effectively enhanced system mud-water separation, and granule sludge has the generation of being difficult for sludge bulking, toxin immunity impact capacity is strong and bear the advantages such as ammonia nitrogen loading height.
(2) than traditional nitrosification activated sludge reactor, the biomass of nitrosated granular sludge reactor is large, microorganism structure is fine and close, while processing high ammonia-nitrogen wastewater, speed of reaction is fast, can reduce reactor volume, hydraulic detention time and reduction second pond volume etc.
Accompanying drawing explanation
Accompanying drawing 1 is sequencing batch activated sludge reactor of the present invention (SBR) schematic diagram: reactor body (1), aerator (2), intake pump (3), go out water solenoid valve (4), time control program (5), distribution reservoir (6).
Accompanying drawing 2 is nitrosated granular sludge photomacrographs that the present invention cultivates.
Embodiment
Below in conjunction with drawings and Examples, the present invention is described in further details.Should be understood that described in following examples it is for the present invention is described, not in order to limit the present invention.
Adopt sequencing batch activated sludge reactor (SBR) domestication to cultivate nitrosated granular sludge, accompanying drawing 1 is shown in by reactor schematic diagram.SBR comprises reactor body (1), aerator (2), intake pump (3), goes out water solenoid valve (4), time control program (5), distribution reservoir (6).Sbr reactor device internal diameter 8cm, virtual height 64cm, aspect ratio is 8:1.Reactor adopts bottom water inlet and bottom micro-pore aeration, and the discharge rate of controlling reactor is 50%.The cotton-shaped active sludge of ammonia oxidation bacteria is rich in reactor inoculation, and seed sludge volume accounts for 30% of reactor cumulative volume.
Adopt simulated wastewater domestication to cultivate aerobic particle mud, C
6h
12o
6for simulation carbon source, NH
4cl is simulation nitrogenous source, KH
2pO
4for simulation phosphorus source, artificial distribution's water quality used is as follows: configuration sbr reactor device water inlet pH is that 7.8, COD concentration is 600 mg/L, the NH of initially intaking
4 +-N concentration is 600mg/L, monthly improves 100mg/L, until constant after 800 mg/L, total phosphorus concentration is 60 mg/L, CaCl
22H
2o 30 mg/L, MgSO
47 H
2o 25 mg/L, FeSO
47 H
2o 20 mg/L.In every liter of simulated wastewater, add 1mL trace element: H
3bO
30.05 g/L, ZnCl
20.05 g/L, CuCl
20.03 g/L, MnSO
4h
2o 0.05 g/L, (NH
4)
6moO
244H
2o 0.05 g/L, AlCl
30.05 g/L, CoCl
26H
2o 0.05 g/L, NiCl
20.05 g/L.Surface rising tolerance 1.5 m/min that control sbr reactor device, dissolved oxygen is 2 mg/L, temperature is 28 ℃, hydraulic detention time 16 h.Operational mode is 6h for controlling a cycle of sbr reactor device, water inlet 5min, and front standing 25min, aeration 300min, the settling time is reduced to 2 min gradually by 15 min, water outlet 5min, all the other are idle mixing time.
Through the domestication of 120 days, reactor was successfully turned out nitrosated granular sludge, and the nitrosated granular sludge color of cultivating is tawny, is shaped as spherical or elliposoidal, and median size is 2.5 mm(accompanying drawings 2), settling velocity is 38 m/h.The 118th day effluent quality of take is example, and nitrosated granular sludge reactor is NH
4 +-N and NO
2 --N concentration is respectively 240.5 and 284.4mg/L, and ratio is 1:1.18, water outlet NO
3 --N concentration is 46.8 mg/L, and pH value is 8.1, and the effluent quality of nitrosated granular sludge reactor meets the water inlet requirement of Anammox.
Claims (4)
1. a cultural method for the nitrosated granular sludge matching with Anammox, is characterized in that comprising the steps:
(1) adopt sequencing batch activated sludge reactor (SBR) domestication to cultivate nitrosated granular sludge, the cotton-shaped active sludge of ammonia oxidation bacteria is rich in reactor inoculation, and seed sludge volume accounts for 30 ~ 50% of reactor cumulative volume;
(2) configuration sbr reactor device water inlet, pH is 7.5 ~ 8.0, inlet COD concentration is 400 ~ 800 mg/L, water inlet NH
4 +-N concentration is 600 ~ 800 mg/L, and water inlet total phosphorus concentration is 50 ~ 80 mg/L;
(3) control surface rising tolerance 1.0 ~ 2.5 m/min of sbr reactor device, dissolved oxygen is 1 ~ 3 mg/L, temperature is 25 ~ 30 ℃, hydraulic detention time 8 ~ 16 h, settling times 2 ~ 15 min, can in 90 ~ 120 days, turn out nitrosated granular sludge, the effluent quality of nitrosated granular sludge reactor meets the water inlet requirement of Anammox.
2. the cultural method of the nitrosated granular sludge that a kind of and Anammox according to claim 1 match, it is characterized in that: in described step (1), sbr reactor device aspect ratio is 5 ~ 10, reactor adopts bottom water inlet and bottom micro-pore aeration, and the discharge rate of controlling reactor is 50%.
3. the cultural method of the nitrosated granular sludge that a kind of and Anammox according to claim 1 match, it is characterized in that: the nitrosated granular sludge color in described step (3) is tawny, be shaped as spherical or elliposoidal, median size is 0.8 ~ 3.0mm, and settling velocity is 20 ~ 50 m/h.
4. the cultural method of the nitrosated granular sludge that a kind of and Anammox according to claim 1 match, is characterized in that: the effluent quality of the nitrosated granular sludge reactor in described step (3) is NH
4 +-N and NO
2 --N concentration ratio is 1:(1 ~ 1.5), NO
3 --N concentration 30 ~ 60 mg/L, pH value is 7.7 ~ 8.5.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104529056A (en) * | 2014-11-29 | 2015-04-22 | 北京工业大学 | Method for realizing autotrophic nitrogen removal of city sewage through symbiosis of flocculent sludge and granular sludge |
| CN104829044A (en) * | 2015-04-18 | 2015-08-12 | 北京工业大学 | Method used for realizing whole-course autotrophic nitrogen removing via anaerobic ammonium oxidation immobilization |
| CN106966492A (en) * | 2017-05-27 | 2017-07-21 | 济南大学 | A kind of method of porous hydroxyapatite bio-carrier Fiber differentiation aerobic particle mud |
| CN107892385A (en) * | 2017-11-22 | 2018-04-10 | 沈阳建筑大学 | A kind of low-temperature lower portion divides the hot housing method of nitrosated granular sludge |
| CN109761346A (en) * | 2019-03-11 | 2019-05-17 | 福建省环境科学研究院（福建省排污权储备和管理技术中心） | The method that biological ceramic particle realizes short distance nitration granular sludge is added under a kind of low organic loading |
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2014
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104529056A (en) * | 2014-11-29 | 2015-04-22 | 北京工业大学 | Method for realizing autotrophic nitrogen removal of city sewage through symbiosis of flocculent sludge and granular sludge |
| CN104529056B (en) * | 2014-11-29 | 2016-04-06 | 北京工业大学 | A kind of floc sludge and granule sludge symbiosis realize the method for municipal effluent autotrophic denitrification |
| CN104829044A (en) * | 2015-04-18 | 2015-08-12 | 北京工业大学 | Method used for realizing whole-course autotrophic nitrogen removing via anaerobic ammonium oxidation immobilization |
| CN106966492A (en) * | 2017-05-27 | 2017-07-21 | 济南大学 | A kind of method of porous hydroxyapatite bio-carrier Fiber differentiation aerobic particle mud |
| CN107892385A (en) * | 2017-11-22 | 2018-04-10 | 沈阳建筑大学 | A kind of low-temperature lower portion divides the hot housing method of nitrosated granular sludge |
| CN107892385B (en) * | 2017-11-22 | 2020-10-16 | 沈阳建筑大学 | Reinforced culture method for partial nitrosation granular sludge at low temperature |
| CN109761346A (en) * | 2019-03-11 | 2019-05-17 | 福建省环境科学研究院（福建省排污权储备和管理技术中心） | The method that biological ceramic particle realizes short distance nitration granular sludge is added under a kind of low organic loading |
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Application publication date: 20140409 |