CN102952764B - Culture method of salt-tolerant anaerobic ammonium oxidation bacteria - Google Patents
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Abstract
The invention provides a culture method of salt-tolerant anaerobic ammonium oxidation bacteria, which comprises the following steps of: A, enrichment culture: performing enrichment culture on the strain to obtain anaerobic ammonium oxidation bacteria; and B, bacteria acclimation culture: gradually increasing the concentration of salt in the culture solution to perform salt tolerance acclimation culture of the anaerobic ammonium oxidation bacteria subjected to the enrichment culture in the step A, wherein the acclimation period of the bacterial at each salt concentration is 15-30 days; and the step A and step B both adopt sequencing batch culture, and the dissolved oxygen value in the culture solution is 0-0.5mg/L. The bacteria cultured by the method provided by the invention are applied to high-salt ammonia nitrogen wastewater, and the total nitrogen removal rate is high. By adopting the method provided by the invention to culture the salt-tolerant anaerobic ammonium oxidation bacteria, the range of available substrates is wide, and the culture is easy to implement; and additional organic carbon source is not required in the bacteria culture and application process, investment is greatly saved, and the wastewater treatment cost is reduced. The bacteria culture method provided by the invention can be applied to large-scale production, and has good economic and environmental benefits.
Description
Technical field
The present invention relates to a kind of cultural method of salt tolerant Anammox flora, more particularly, relate to a kind of enrichment culture method of the Anammox flora taking ammonia nitrogen in high-salt wastewater as handling object, belong to microbial technology field, specifically can be applicable to process the contour salt ammonia nitrogen of catalyzer industry and Nitrogenous Fertilizer Industry trade effluent.
Background technology
Ammonia nitrogen is important factor in water pollution, and ammonia nitrogen too high in water easily causes the excessive multiplication of aquatic plant and algae, and self purification of water body ability is weakened, and causes body eutrophication thereby worsen water quality.In the prior art, the main improvement method of ammonia nitrogen waste water has physico-chemical process, advanced oxidation processes and biological process.
Wherein, physico-chemical process has break point chlorination, chemical precipitation method, ion exchange method, stripping/vaporizing extract process, membrane technique method, electroosmose process and crystallization process; There is the shortcoming such as severe reaction conditions, working cost height in physico-chemical process; Physico-chemical process also only can be applied to the wastewater treatment in part industry and field in addition.Advanced oxidation processes mainly comprises photocatalytic oxidation, supercritical catalyst oxidation style, wet oxidation process and catalytic wet air oxidation; The realization of advanced oxidation processes all has that facility investment is large, working cost is high, operational condition requires harsh feature, and the ammonia nitrogen in ammonia nitrogen waste water is converted to oxynitride after high-level oxidation technology, and its total nitrogen still needs further processing.And biological process processing ammonia nitrogen waste water also has following various ways, as nitrated in tradition/denitrification method, Nitritation/denitrification method, Simultaneous Nitrification/denitrification method, nitrosification/Anammox method; Biological process is because its processing cost is low, effective, thus widely applicable being widely applied.
Nitrosification/Anammox method is processed ammonia nitrogen waste water, refers to that ammonia nitrogen waste water is first nitroso-group nitrogen through half way nitrosification by part mineralized nitrogen, then removes total nitrogen through Anammox processing.Wherein, nitrosification process is carried out under the nitrosification flora effect of cultivating, and same anaerobic ammonium oxidation process is to carry out under the Anammox flora effect of cultivating, and spawn culture finishes the rear nitrosification/anaerobic ammonia oxidation process that is all applied to.
Anaerobic ammonia oxidizing bacteria is the newfound anaerobism autotroph of a class, and it can change into nitrogen according to a certain percentage by ammonia nitrogen and nitrite nitrogen, and its scientific research value and industrial value are all good.
High salt ammonia nitrogen waste water refers to the ammonia nitrogen waste water that total saliferous massfraction is greater than 1%, due to existence high in salt in waste water, microorganism cells film and endobacillary enzyme can be destroyed, and microbial growth is also suppressed, thereby increased the difficulty of ammonia nitrogen waste water biological treatment.Method to biochemical treatment after first dilution of the general employing of high salt ammonia nitrogen waste water in prior art; But the dilution step containing in the method will increase treatment scale, initial cost and the working cost of ammonia nitrogen waste water.
In prior art, patent CN101195803A discloses a kind of anaerobic ammonoxidation bacterium concentrating device, and it is mainly that a kind of new anaerobic ammonoxidation bacterium concentrating culture apparatus is described.Patent CN101205526A discloses a kind of method of upflow anaerobic sludge blanket reactor quickly culturing anaerobic ammonia oxidation bacteria, it processes active sludge in percolate SBR technique as seed sludge using refuse landfill, taking ammonia nitrogen, micro-inorganic salt and damping fluid as nutrient solution, control NH
4 +-N and NO
2 -the method that-N ratio and control pH value are cultivated.
Dilution method while processing high salt ammonia nitrogen waste water in prior art can not be satisfied the demand, and becomes a technical barrier and how to cultivate the Anammox flora that adapts to high salinity ammonia nitrogen waste water.
Summary of the invention
The cultural method that the invention provides a kind of salt tolerant Anammox flora, comprising: steps A, enrichment culture: be Anammox flora by bacterial classification enrichment culture; With step B, flora domestication is cultivated: take the method that improves step by step salt concn in nutrient solution to carry out salt tolerant domestication cultivation to the Anammox flora of enrichment culture in steps A, flora is 15～30 days at the acclimation period of each salt concn; Wherein steps A and step B are sequence batch (cultivation, and in its nutrient solution, oxygen dissolving value is 0～0.5mg/L.
According to the present invention, in preferred described step B, the initial salt concn of nutrient solution is 8～12g/L, and every grade increases progressively salt concn gradient is 4～8g/L, and final salt concn is 20～24g/L.More preferably in step B, the initial salt concn of nutrient solution is 10.5～12g/L, and every grade increases progressively salt concn gradient is 5.5～6g/L, and final salt concn is 22～23g/L.
According to a preferred embodiment of the present invention, in described steps A enrichment culture liquid, ammonia nitrogen concentration is 50～200mg/L, and nitrite nitrogen concentration is 50～250mg/L, and the concentration of phosphorus is 10～300mg/L.Equally, in described step B flora domestication nutrient solution, ammonia nitrogen concentration is 150～200mg/L, and nitrite nitrogen concentration is 150～250mg/L, and the concentration of phosphorus is 10～300mg/L, and in step B, used salt is sodium-chlor and/or sodium sulfate.
Preferably in described steps A of the present invention, in the nutrient solution of enrichment culture, ammonia nitrogen concentration, for progressively increasing step by step, comprises one or more cycles of operation under each ammonia nitrogen concentration; Wherein each cycle of operation is 24～48h, comprises that all the other times are lay-up period into water 30min, anaerobic reaction 21～44h, precipitation 1～2h, draining 1h.Equally, in described step B, the domestication of each salinity comprises multiple cycles of operation; Wherein each cycle of operation is 24～48h, comprises that all the other times are lay-up period into water 30min, anaerobic reaction 21～44h, precipitation 1～2h, draining 1h.
Preferably in described steps A of the present invention, bacterial classification derives from active sludge, and the concentration of inoculation of activated-sludge is controlled at 1～3g/L; Total cycle of steps A enrichment culture Anammox flora is 20～80 days.
In the present invention, equal not additional organic carbon source in preferred described steps A and B, is carbon source and use inorganic carbonate.
The present invention also provide a kind of according to the present invention in the aforesaid method application of salt tolerant Anammox flora in high salt ammonia nitrogen waste water of cultivating, wherein high salt ammonia nitrogen waste water refers to the waste water that total saliferous >=1%, ammonia-nitrogen content are 50～450mg/L.
Compared with prior art, tool has the following advantages in the present invention.First, in the present invention, in salt tolerant Anammox flora culturing process, can utilize substrate scope extensively and be easy to cultivate; In bacteria group culture and application process, all do not need additional organic carbon source, greatly saved investment, thereby reduced cost for wastewater treatment.Secondly, the salt tolerant Anammox flora concentration high (half an hour, settling ratio was 30～50%) after cultivating, its biological activity is good; Be applicable to various ammonia nitrogen Industrial Wastewater Treatment, and can significantly improve the nitrogen removal rate of high salt ammonia nitrogen waste water.Again, the cultural method using is applicable to large-scale production, and has good economic benefit and environmental benefit.
Brief description of the drawings
Fig. 1 is the biological fermentation tank schematic diagram that sequence batch (is cultivated salt tolerant Anammox flora.
Embodiment
Described method of the present invention comprises enrichment culture and flora domestication two stages of cultivation; Flora after domestication cultivation finishes is for the treatment of high salt ammonia nitrogen waste water.
When concrete enforcement, in the enrichment culture of steps A: to inoculation of activated-sludge (wherein several microbial bacteria specific admixture) in the biological fermentation tank with heating unit, its inoculum density is 1～3g/L, and training method is that sequence batch ((intermittent type) is cultivated; Enrichment culture is carried out in simulated wastewater.Simulated wastewater is conventional inorganic nutrient salts such as adding iron, magnesium, potassium, phosphorus in tap water, and adding ammonium salt and nitrosonium salts is nitrogenous substrate, and interpolation inorganic carbonate is that carbon source forms.In described biological fermentation tank, temperature is controlled at 25～38 DEG C; Increase mass transfer velocity by stirring at low speed, rotating speed < 60r/min, makes oxygen dissolving value in fermentor tank be controlled at 0～0.5mg/L, and preferable range is 0～0.2mg/L.In described simulated wastewater, ammonia nitrogen concentration is 50～200mg/L, and preferred concentration is 100～200mg/L; Nitrite nitrogen concentration is 50～250mg/L, and preferred concentration is 100～250mg/L; And NH
4 +-N and NO
2 --N ratio is controlled at 1～1.5; Preferably in the time that retentive control ammonia nitrogen and nitrite nitrogen concentration are in above-mentioned scope, adopt method (concentration incremental gradient is 30～50mg/L) the enrichment culture Anammox flora that progressively improves ammonia nitrogen and nitrite nitrogen concentration, to accelerate its enrichment culture process.In enrichment culture process, do not need additional any organic carbon source, wherein volatile salt had not only added in simulated wastewater as ammonia nitrogen substrate but also as part carbon source substrate.Fe in inorganic nutrient salt
2+mainly with FeSO
47H
2o salt adds, and its concentration is controlled at 2～20mg/L, is preferably 5～15mg/L; Mg
2+mainly with MgSO
47H
2o salt adds, and its concentration is controlled at 5～20mg/L, is preferably 10～15mg/L; Phosphorus is mainly with KH
2pO
4or K
2hPO
4salt adds, and its concentration is controlled at 10～300mg/L, is preferably 50～200mg/L.And in simulated wastewater, adding appropriate sodium bicarbonate carbon source as a supplement, in fermentor tank, pH mainly acts synergistically to regulate by dilute sulphuric acid and sodium bicarbonate.Be that sodium bicarbonate is on the one hand as part carbon source substrate, on the other hand as pH buffering salt.PH in fermentor tank is controlled between 6.0～9.0, and preferable range is 7.5～8.5.In this biological fermentation tank, each cycle of operation is 24～48h, comprises that all the other times are lay-up period into water 30min, anaerobic reaction 21～44h, precipitation 1～2h, draining 1h.Total cycle of enrichment culture Anammox flora is 20～80 days.Flora concentration after enrichment culture is also 1～3g/L, but flora now belongs to purer Anammox flora, and effectively bacterium is dense is 1～3g/L.
In step B, start salt tolerant domestication process through the Anammox flora of enrichment culture.In this process, ammonia nitrogen concentration control is 150～200mg/L; Nitrite nitrogen concentration is 150～250mg/L; Total salt concentration >=0.8%; In the simulated wastewater of domestication, all the other compositions are identical with steps A.Step B adopts the method that progressively increases salinity to tame Anammox flora.The domestication starting stage, in simulated wastewater, sodium-chlor and sodium sulfate add according to 8～12g/L; In domestication process after this, in simulated wastewater, to increase progressively salt concn gradient be 4～8g/L for every grade, sodium-chlor and sodium sulfate; After two-stage or multistage increasing progressively, final salt concn is 20～24g/L.Wherein, the acclimation period of each salinity is approximately 15～30d, is preferably 20～30d; In the acclimation period of each salinity, pass through the biological fermentation tank cycle of operation for several times; The biological fermentation tank each run cycle is 24～48h, comprises that all the other times are lay-up period into water 30min, anaerobic reaction 21～44h, precipitation 1～2h, draining 1h.At the flora domestication initial stage, in mud, still contain protozoon, especially more with campanularian and wheel animalcule quantity.Along with the carrying out of domestication process, protozoon cannot adapt to hypersaline environment, and its value volume and range of product in treatment system obviously reduces until disappear.After domestication finishes, the mud granule forming is little and closely knit, and zoogloea becomes the closely knit bulk of bulk (diameter is about 0.5～1.5mm); In mud, do not observe aerobic thread fungus and exist, mud color is the sorrel of Anammox flora.
In experiment, when we finish by the proterties in the flora enrichment of observation Anammox and domestication and by enrichment and each cycle of operation of domestication, flora judges to the treatment effect of ammonia nitrogen waste water whether enrichment and flora domestication process complete.After final high salinity domestication, the NH in high salinity simulated wastewater
4 +-N, NO
2 --N and TN clearance in 24～48h reaches respectively more than 98.0%, 96.5% and 76.5%.
Salt tolerant Anammox flora after domestication is cultivated is applied to industrial high salt ammonia nitrogen waste water, and the water quality of the described high salt ammonia nitrogen waste water of handling object industry is as table 1.As the ammonia nitrogen of 50～450mg/L in table 1 trade effluent contains ammonia nitrogen 50～200mg/L and nitrite nitrogen 50～250mg/L after half way nitrosification, now can apply salt tolerant Anammox flora of the present invention and remove total nitrogen.
Table 1
Below in conjunction with the drawings and specific embodiments, the present invention is described in further detail.Below be only the preferred embodiment of the present invention, protection scope of the present invention is not limited to this, and any those skilled in the art is in technical scope disclosed by the invention, within can being easy to the change carried out or changing and be encompassed in protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of claims.
Embodiment 1
Steps A, Anammox flora enrichment culture: take from the active sludge of certain chemical plant sewage treatment plant anaerobic pond as bacterial classification, with 2.5g/L concentration, active sludge is seeded in the biological fermentation tank shown in Fig. 1 to the consisting of of simulated wastewater in biological fermentation tank: NH
4 +-N (NH
4hCO
3) 100～200mg/L; NO
2 --N (NaNO
2) 100～250mg/L; Fe
2+(FeSO
47H
2o) 12mg/L; Mg
2+(MgSO
47H
2o) 20mg/L; P (KH
2pO
4) 65mg/L; Be (0.5～20) according to carbon-nitrogen ratio: 1 adds sodium bicarbonate.Oxygen dissolving value in fermentor tank is controlled to 0～0.5mg/L, and pH is controlled between 7.5～8.5, and temperature is controlled at 25～38 DEG C.The biological fermentation tank each run cycle is 48h, comprises that all the other times are lay-up period into water 30min, anaerobic reaction 44h, precipitation 2h, draining 1h.The concrete operations of biological fermentation tank each run are: in opening for feed 1 as shown in Figure 1, inject simulated wastewater and access bacterial classification formation nutrient solution 8, turn on agitator 7 is carried out stirring reaction, its rotating speed is controlled by electric motor 2, thereby oxygen dissolving value is controlled to 0～0.5mg/L ensureing that muddy water mixes under condition; Detect and control mouthful 6 places at pH pH is controlled between 7.5～8.5, flow into and the chuck recirculated water that flows out from circulating water outlet 5 is controlled at temperature between 25～38 DEG C from circulating water intake 4.After opening for feed 1 is sealed, carry out anaerobic reaction, the waste gas producing in anaerobic reaction process is discharged by venting port 3, and after precipitation, denitrogenation waste water is discharged from drain hole 9, and isolated active sludge continues to use in the next cycle of operation.Total cycle of enrichment culture Anammox flora is 60 days.
Step B, the domestication of salt tolerant Anammox flora is cultivated: complete after Anammox flora enrichment culture, enter salt tolerant Anammox flora domestication culturing process.The domestication starting stage, in simulated wastewater, sodium-chlor and sodium sulfate add according to 1.5g/L and 6.5g/L respectively; After this in domestication process, in waste water, sodium-chlor and sodium sulfate concentration increase progressively according to 1.5g/L and 2.5g/L concentration gradient respectively, and after Pyatyi salinity domestication is cultivated, its final salt concn is respectively 7.5g/L and 16.5g/L.The acclimation period of each salinity is 16d; In biological fermentation tank, each cycle of operation is 48h, comprises that all the other times are lay-up period into water 30min, anaerobic reaction 44h, precipitation 2h, draining 1h.After domestication finishes, zoogloea becomes bulk bulk, does not find that thread fungus and protozoon value volume and range of product are little in mud, and mud granule is little and closely knit.In domestication simulated wastewater, the ammonia nitrogen of each cycle of operation can be removed more than 98% in 48h.So far think that salt tolerant Anammox flora enrichment culture and domestication cultivation finish.
Embodiment 2
Steps A, Anammox flora enrichment culture: take from the active sludge of certain sewage work's oxygen compatibility pool testing apparatus as bacterial classification, with 2g/L concentration, active sludge is seeded in the biological fermentation tank shown in Fig. 1, the consisting of of simulated wastewater in biological fermentation tank: NH
4 +-N (NH
4hCO
3) 50～150mg/L; NO
2 --N (NaNO
2) 50～200mg/L; Fe
2+(FeSO
47H
2o) 10mg/L; Mg
2+(MgSO
47H
2o) 15mg/L; P (KH
2pO
4) 60mg/L; Be (0.5～20) according to carbon-nitrogen ratio: 1 adds sodium bicarbonate.Oxygen dissolving value in fermentor tank is controlled to 0～0.5mg/L, and pH is controlled between 7.0～8.5, and temperature is controlled at 25～38 DEG C.The biological fermentation tank each run cycle is 24h, comprises that all the other times are lay-up period into water 30min, anaerobic reaction 21h, precipitation 1.4h, draining 1h.Total cycle of enrichment culture Anammox flora is 40 days.
Step B, the domestication of salt tolerant Anammox flora is cultivated: complete after Anammox flora enrichment culture, enter salt tolerant Anammox flora domestication culturing process.The domestication starting stage, in simulated wastewater, sodium-chlor and sodium sulfate add according to 1.5g/L and 6.5g/L respectively; After this in domestication process, in waste water, sodium-chlor and sodium sulfate concentration increase progressively according to 1.5g/L and 2.5g/L concentration gradient respectively, and after level Four salinity domestication is cultivated, its final salt concn is respectively 6g/L and 14g/L.The acclimation period of each salinity is 18d; The biological fermentation tank each run cycle is 24h, comprises that all the other times are lay-up period into water 30min, anaerobic reaction 21h, precipitation 1.4h, draining 1h.After domestication finishes, zoogloea becomes bulk bulk, does not find that thread fungus and protozoon value volume and range of product are little in mud, and mud granule is little and closely knit.In domestication simulated wastewater, the ammonia nitrogen of each cycle of operation can be removed more than 97% in 24h.So far think that salt tolerant Anammox flora enrichment culture and domestication cultivation finish.
Embodiment 3
In the present embodiment, steps A Anammox flora enrichment culture process is identical with embodiment 1.
Step B, the domestication of salt tolerant Anammox flora is cultivated: complete after Anammox flora enrichment culture, enter salt tolerant Anammox flora domestication culturing process.The domestication starting stage, in simulated wastewater, sodium-chlor and sodium sulfate add according to 1.5g/L and 6.5g/L respectively; After this in domestication process, in waste water, sodium-chlor and sodium sulfate concentration increase progressively according to 2.5g/L and 3.5g/L concentration gradient respectively, and after three grades of salinity domestications are cultivated, its final salt concn is respectively 6.5g/L and 13.5g/L.The acclimation period of each salinity is 20d; In biological fermentation tank, each cycle of operation is 48h, comprises that all the other times are lay-up period into water 30min, anaerobic reaction 44h, precipitation 2h, draining 1h.After domestication finishes, zoogloea becomes bulk bulk, does not find that thread fungus and protozoon value volume and range of product are little in mud, and mud granule is little and closely knit.In domestication simulated wastewater, the ammonia nitrogen of each cycle of operation can be removed more than 98% in 48h.So far think that salt tolerant Anammox flora enrichment culture and domestication cultivation finish.
Embodiment 4
In the present embodiment, steps A Anammox flora enrichment culture process is identical with embodiment 1.
Step B, the domestication of salt tolerant Anammox flora is cultivated: complete after Anammox flora enrichment culture, enter salt tolerant Anammox flora domestication culturing process.The domestication starting stage, in simulated wastewater, sodium-chlor and sodium sulfate add according to 3.5g/L and 8.5g/L respectively; After this in domestication process, in waste water, sodium-chlor and sodium sulfate concentration increase progressively according to 3.5g/L and 4.5g/L concentration gradient respectively, and after two-stage salinity domestication is cultivated, its final salt concn is respectively 7.0g/L and 13.0g/L.The acclimation period of each salinity is 30d; In biological fermentation tank, each cycle of operation is 48h, comprises that all the other times are lay-up period into water 30min, anaerobic reaction 44h, precipitation 2h, draining 1h.After domestication finishes, zoogloea becomes bulk bulk, does not find that thread fungus and protozoon value volume and range of product are little in mud, and mud granule is little and closely knit.In domestication simulated wastewater, the ammonia nitrogen of each cycle of operation can be removed more than 96% in 48h.So far think that salt tolerant Anammox flora enrichment culture and domestication cultivation finish.
Embodiment 5
In the present embodiment, steps A Anammox flora enrichment culture process is identical with embodiment 1.
Step B, the domestication of salt tolerant Anammox flora is cultivated: complete after Anammox flora enrichment culture, enter salt tolerant Anammox flora domestication culturing process.The domestication starting stage, in simulated wastewater, sodium-chlor and sodium sulfate add according to 3.5g/L and 8.5g/L respectively; After this in domestication process, in waste water, sodium-chlor and sodium sulfate concentration increase progressively according to 2.5g/L and 3g/L concentration gradient respectively, and after three grades of salinity domestications are cultivated, its final salt concn is respectively 8.5g/L and 14.5g/L.The acclimation period of each salinity is 24d; In biological fermentation tank, each cycle of operation is 48h, comprises that all the other times are lay-up period into water 30min, anaerobic reaction 44h, precipitation 2h, draining 1h.After domestication finishes, zoogloea becomes bulk bulk, does not find that thread fungus and protozoon value volume and range of product are little in mud, and mud granule is little and closely knit.In domestication simulated wastewater, the ammonia nitrogen of each cycle of operation can be removed more than 98% in 48h.So far think that salt tolerant Anammox flora enrichment culture and domestication cultivation finish.
Embodiment 6
In the present embodiment, steps A Anammox flora enrichment culture process is identical with embodiment 1.
Step B, the domestication of salt tolerant Anammox flora is cultivated: complete after Anammox flora enrichment culture, enter salt tolerant Anammox flora domestication culturing process.The domestication starting stage, in simulated wastewater, sodium-chlor and sodium sulfate add according to 2.5g/L and 8g/L respectively; After this in domestication process, in waste water, sodium-chlor and sodium sulfate concentration increase progressively according to 2.0g/L and 4.0g/L concentration gradient respectively, and after three grades of salinity domestications are cultivated, its final salt concn is respectively 6.5g/L and 16g/L.The acclimation period of each salinity is 22d; In biological fermentation tank, each cycle of operation is 48h, comprises that all the other times are lay-up period into water 30min, anaerobic reaction 44h, precipitation 2h, draining 1h.After domestication finishes, zoogloea becomes bulk bulk, does not find that thread fungus and protozoon value volume and range of product are little in mud, and mud granule is little and closely knit.In domestication simulated wastewater, the ammonia nitrogen of each cycle of operation can be removed more than 98% in 48h.So far think that salt tolerant Anammox flora enrichment culture and domestication cultivation finish.
Comparative example 1
In this comparative example, steps A Anammox flora enrichment culture process is identical with embodiment 1.
Step B, as different from Example 1: complete after enrichment culture, do not adopt the method that progressively improves salt concn to carry out salt tolerance domestication to Anammox flora, but directly in simulated wastewater, adding sodium-chlor and sodium sulfate according to the concentration of 7.5g/L, 16.5g/L respectively, other culture condition are identical with the step B of embodiment 1.After cultivation finishes, in simulated wastewater there are no bulk bulk zoogloea, clearance≤70% in final outflow water ammonia nitrogen 48h.
Compared with embodiment 1, the ammonia nitrogen treatment effect of comparative example 1 is obviously poor.Illustrate and do not adopt the method that progressively improves nutrient solution salt concn to carry out salt tolerance domestication cultivation to Anammox flora, the Anammox flora obtaining is difficult to adapt to high salt concentration scope.
Claims (9)
1. a cultural method for salt tolerant Anammox flora, comprising:
Steps A, enrichment culture: be Anammox flora by bacterial classification enrichment culture;
Step B, flora domestication is cultivated: take the method that improves step by step salt concn in nutrient solution to carry out salt tolerant domestication cultivation to the Anammox flora of enrichment culture in steps A, flora is 15～30 days at the acclimation period of each salt concn;
Wherein steps A and step B are sequence batch (cultivation, and in its nutrient solution, oxygen dissolving value is 0～0.5mg/L; And in step B, the initial salt concn of nutrient solution is 8～12g/L, and every grade increases progressively salt concn gradient is 4～8g/L, final salt concn is 20～24g/L.
2. cultural method according to claim 1, is characterized in that: in described step B, the initial salt concn of nutrient solution is 10.5～12g/L, and every grade increases progressively salt concn gradient is 5.5～6g/L, and final salt concn is 22～23g/L.
3. cultural method according to claim 1 and 2, is characterized in that: in the enrichment culture liquid of described steps A, ammonia nitrogen concentration is 50～200mg/L, and nitrite nitrogen concentration is 50～250mg/L, and the concentration of phosphorus is 10～300mg/L.
4. cultural method according to claim 1 and 2, it is characterized in that: in described step B flora domestication nutrient solution, ammonia nitrogen concentration is 150～200mg/L, nitrite nitrogen concentration is 150～250mg/L, and the concentration of phosphorus is 10～300mg/L, and in step B, used salt is sodium-chlor and/or sodium sulfate.
5. cultural method according to claim 1 and 2, is characterized in that: in described steps A, in the nutrient solution of enrichment culture, ammonia nitrogen concentration, for progressively increasing step by step, comprises one or more cycles of operation under each ammonia nitrogen concentration; Wherein each cycle of operation is 24～48h, comprises that all the other times are lay-up period into water 30min, anaerobic reaction 21～44h, precipitation 1～2h, draining 1h.
6. cultural method according to claim 1 and 2, is characterized in that: in described step B, the domestication of each salinity comprises multiple cycles of operation; Wherein each cycle of operation is 24～48h, comprises that all the other times are lay-up period into water 30min, anaerobic reaction 21～44h, precipitation 1～2h, draining 1h.
7. cultural method according to claim 1 and 2, is characterized in that: in described steps A, bacterial classification derives from active sludge, and the concentration of inoculation of activated-sludge is controlled at 1～3g/L; Total cycle of steps A enrichment culture Anammox flora is 20～80 days.
8. cultural method according to claim 1 and 2, is characterized in that: equal not additional organic carbon source in described steps A and B is carbon source and use inorganic carbonate.
9. the application of the salt tolerant Anammox flora that the cultural method as described in any one in claim 1～8 is cultivated in high salt ammonia nitrogen waste water, wherein high salt ammonia nitrogen waste water refers to the waste water that total saliferous >=1%, ammonia-nitrogen content are 50～450mg/L.
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| CN103288223B (en) * | 2013-06-09 | 2014-04-09 | 北京工业大学 | Ammonia oxidizing bacterium enriching device and method suitable for purifying micro-polluted water and application of ammonia oxidizing bacterium enriching device and method |
| CN104609542B (en) * | 2013-11-05 | 2017-10-03 | 中蓝连海设计研究院 | A kind of method for cultivating salt tolerant aerobic activated sludge |
| CN104016478B (en) * | 2014-06-25 | 2016-07-06 | 大连理工大学 | A kind of method of quick startup anaerobic ammonia oxidation reactor |
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