CN103288223B - Ammonia oxidizing bacterium enriching device and method suitable for purifying micro-polluted water and application of ammonia oxidizing bacterium enriching device and method - Google Patents
Ammonia oxidizing bacterium enriching device and method suitable for purifying micro-polluted water and application of ammonia oxidizing bacterium enriching device and method Download PDFInfo
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- CN103288223B CN103288223B CN201310232063.5A CN201310232063A CN103288223B CN 103288223 B CN103288223 B CN 103288223B CN 201310232063 A CN201310232063 A CN 201310232063A CN 103288223 B CN103288223 B CN 103288223B
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Abstract
The invention discloses an ammonia oxidizing bacterium enriching device and method suitable for purifying micro-polluted water and an application of the ammonia oxidizing bacterium enriching device and method, belonging to the technical field of feed water treatment. The device comprises an ammonia oxidizing bacterium enriching reactor, an ammonia oxidizing bacterium culture solution storage tank and an ammonia oxidizing bacterium nutrition control system. Ammonia oxidizing bacteria are inoculated, an ammonia oxidizing bacterium culture solution is used as the inflow water of the ammonia oxidizing bacterium enriching reactor, and the ammonia oxidizing bacterium nutrition control system is used for maintaining the dissolved oxygen concentration at 5-7mg/L, the pH value at 8.0-8.5 and the ammonia nitrogen concentration at 4-8 mg/L in the culture process, so that an ammonia oxidizing bacterium biofilm grows and attaches a polyethylene cylinder of the ammonia oxidizing bacterium enriching reactor. The concentration of ammonia oxidizing bacteria enriched by using the device and the method is high; when the device and the method are applied to a raw water pipe or water treatment process unit of micro-polluted water, the biological nitrogen removal effect can be effectively improved, and the ammonia nitrogen removal load of a water plant is greatly relieved; the device and the method have the advantages of simple operation, short enriching time and convenience for applying strains.
Description
Technical field
The invention belongs to water-treatment technology field, particularly a kind of ammonoxidation bacterium concentrating device, method and application that is applicable to micro-polluted water purification.
Background technology
At present Chinese Urbanization, industrialization development are rapid, simultaneously owing to lacking effective supervision and management, the pollutent that makes to enter in Natural Water presents increase trend, the eutrophication of water body is one of current problem demanding prompt solution, the eutrophication of water body not only makes a very bad impression to environment, has more directly increased the depollution load of follow-up water purification plant.
Recent study is found, nitrification easily occurs in the high water supply network of ammonia-nitrogen content, to water quality, has brought a series of disadvantageous effects, and the denitrogenation therefore strengthening in Micropolluted watD water pipe or water technology seems particularly important.The aspect of sewage disposal at present, biological denitrificaion is economical and effective the most; And the research of the denitride technology of water purification aspect is less, water purification conventional water treatment process is less to the removal effect of ammonia nitrogen, and the research that makes full use of former water water pipe biologica denitrification also rarely has report.If the method for biological denitrificaion can be applied in the former water water pipe and water technology of water purification plant, so to alleviate water factory except ammonia nitrogen loading, reduce processing cost, to improve water supply quality significant.
The biological activity that in bio-denitrification technology, ammonia oxidation bacteria is good is prerequisite and the guarantee of biological denitrificaion, if ammonia oxidation bacteria content is low or ammonia oxidation bacteria is suppressed, nitration reaction cannot be carried out so, thereby biological denitrificaion just cannot be realized.In nitration reaction, ammonia oxidation bacteria is nitrite nitrogen by mineralized nitrogen, and Nitromonas is converted into nitrate nitrogen by nitrite nitrogen, both are autotrophy aerobic bacteria, poor growth, and in tap water conventional treatment process, proportion is less, finished product bacterial classification is expensive, is difficult for preserving and being applied to engineering practice.Ammonia oxidation bacteria is the limiting factor of nitration reaction, and in sewage disposal, the beneficiation technologies of ammonia oxidation bacteria is comparatively ripe, and the ammonia oxidation bacteria of active sludge of conventionally take is bacterial classification source.Yet source water is micro-polluted water matter, there is very big-difference with sewage quality, if take active sludge as bacterial classification source, active sludge on the impact of water quality much larger than nitrification the cleaning action to water quality, and the Pseudomonas that ammonia oxidation bacteria adapts under different environment is different.In sewage disposal, biological denitrificaion adopts activated sludge process more, and the method denitrification effect is good, but the discharge of excess sludge and processing bring new problem.Therefore, seek a kind of ammonoxidation bacterium concentrating device and method that is applicable to micro-polluted water purification and seem particularly important.
Summary of the invention
For solving existing ammonoxidation bacterium concentrating culture apparatus and method, cannot be applied to the problem that micro-polluted water purifies, the invention provides a kind of ammonoxidation bacterium concentrating device, method and application that micro-polluted water purifies that be applicable to, these apparatus and method can realize the fast enriching of ammonia oxidation bacteria, ammonia oxidation bacteria after enrichment is applied in the former water water pipe or water technology of micro-polluted water, to ammonia nitrogen removal frank in micro-polluted water, can reach more than 83%, these apparatus and method also can be applicable to the basic test research to ammonia oxidation bacteria in micro-polluted water.
For achieving the above object, technical scheme of the present invention is as follows:
Be applicable to the ammonoxidation bacterium concentrating device that micro-polluted water purifies, mainly by ammonoxidation bacterium concentrating reactor (1), ammonia oxidation bacteria nutrient solution holding tank (2) and ammonia oxidation bacteria nutritional control system (A), formed.
Ammonoxidation bacterium concentrating reactor (1) can make ammonoxidation bacterium concentrating growth, top is provided with motor (3), inside is provided with rotor (4), rotor (4) top and bottom are provided with the cross frame (5) of parallel bottom surface, between two cross frames (5), vertical direction is fixed with coaxial polythene drum (6), rotor (4) can rotate under the driving of direct-current machine (3), and drive cross bar (5) to rotate the rotation that realizes polythene drum (6), the rotation of polythene drum (6) is conducive to biomembranously adhere to fast, ammonoxidation bacterium concentrating reactor (1) is connected with ammonia oxidation bacteria nutrient solution holding tank (2) via intake pump (7), can realize the feed liquor of ammonoxidation bacterium concentrating reactor (1), ammonoxidation bacterium concentrating reactor (1) top sets out water pipe (8), adopt the draining of overflow mode, bottom is provided with blow-down pipe (9).
Ammonia oxidation bacteria nutrient solution holding tank (2) is for depositing ammonia oxidation bacteria nutrient solution, ammonia oxidation bacteria nutrient solution holding tank (2) is communicated with ammonoxidation bacterium concentrating reactor (1) by water inlet pipe (10) via intake pump (7), it is the water feed apparatus of ammonoxidation bacterium concentrating reactor (1), intake pump (7) is set on water inlet pipe (10), and ammonia oxidation bacteria nutrient solution holding tank (2) bottom is provided with blow-down pipe (11).
Ammonia oxidation bacteria nutritional control system (A) can make ammonoxidation bacterium concentrating reactor (1) maintain suitable dissolved oxygen concentration value, pH value, ammonia nitrogen concentration value, is beneficial to the growth of ammonia oxidation bacteria.Ammonia oxidation bacteria nutritional control system (A) comprises controller (12), dissolved oxygen meter (13), dissolved oxygen electrode (14), pH determinator (15), pH electrode (16), online ammonia nitrogen determination instrument (17), air compressor (18), add alkali pump (19) and add alkali tank (20), add ammonia pump (21) and ammonification tank (22), wherein adding alkali tank (20) is connected with ammonia oxidation bacteria nutrient solution holding tank (2) by adding alkali pump (19), ammonification tank (22) is connected with ammonia oxidation bacteria nutrient solution holding tank (2) by adding ammonia pump (21), air compressor (18) is connected with ammonia oxidation bacteria nutrient solution holding tank (2) by aeration tube, aeration head (23) is placed in ammonia oxidation bacteria nutrient solution holding tank (2) bottom, the input port (24) of controller (12) is connected with ammonoxidation bacterium concentrating reactor (1) with dissolved oxygen electrode (14), pH electrode (16) by dissolved oxygen meter (13), pH determinator (15) respectively, and same Time Controller (12) is also connected with ammonoxidation bacterium concentrating reactor (1) by online ammonia nitrogen determination instrument (17), the output port (25) of controller (12) by electromagnetic relay (26) respectively with air compressor (18), add alkali pump (19), add ammonia pump (21) and be connected.
Adopt above-mentioned device to carry out ammonoxidation bacterium concentrating method, carry out according to the following steps:
(1) adopt tap water preparation ammonia oxidation bacteria nutrient solution.
(2) prepare ammonia oxidation bacteria inoculation liquid, the gac turbid solution that contains ammonia oxidation bacteria is prepared into ammonia oxidation bacteria suspension, and (this is for prior art, as can be by the people such as Zhou Lingling (Zhou Lingling, Zhang Yongji, Yang Yanling, Li Guibai, a kind of measuring method of amount of colonies of adhesivity heterotrophic bacteria, ZL200810064710.5) to adhering to the treatment process of heterotrophic bacterium) get its supernatant liquor, supernatant liquor is inoculated into the volume ratio of 1:6 in the ammonia oxidation bacteria nutrient solution of step (1) of sterilizing, putting into 28 ℃ of incubator concussions cultivates, rotating speed is 150r/min, every day hydrogen make-up sodium oxide and ammonium chloride, making pH value is 8.0, ammonia nitrogen concentration is 2mg/L, take 3 days as a generation, after one culture finishes, the volume ratio with 1:3 proceeds in step (1) the ammonia oxidation bacteria nutrient solution to sterilizing again, the like, needed altogether for 4 generations, so that ammonia oxidation bacteria enlarged culturing, suppress the growth of heterotrophic bacterium simultaneously,
(3) preparation ammonium chloride solution and sodium hydroxide solution are also injected into respectively ammonification tank and add alkali tank, the ammonia oxidation bacteria nutrient solution of step (1) preparation is injected into ammonia oxidation bacteria nutrient solution holding tank, while injecting first, the ammonia oxidation bacteria nutrient solution of the ammonia oxidation bacteria inoculation liquid of step (2) and step (1) should be inoculated into ammonia oxidation bacteria in the ammonia oxidation bacteria nutrient solution of step (1) volume ratio with 1:10.
(4) start intake pump the ammonia oxidation bacteria nutrient solution in ammonia oxidation bacteria nutrient solution holding tank is introduced to ammonoxidation bacterium concentrating reactor, then open ammonoxidation bacterium concentrating reactor motor, air compressor, controller, add alkali pump, add ammonia pump, dissolved oxygen meter, pH determinator and online ammonia nitrogen determination instrument, keeping maintaining dissolved oxygen concentration in ammonoxidation bacterium concentrating reactor is that 5～7mg/L, ammonia nitrogen concentration are that 4～8mg/L, pH value are 8.0～8.5, and enrichment process starts.
(5) after enrichment process starts, apposition growth microbial film gradually on the polythene drum in ammonoxidation bacterium concentrating reactor;
(6), from step (5), constantly supplement and add liquid in alkali tank, ammonification tank, ammonia oxidation bacteria nutrient solution holding tank, to guarantee adding continuously of liquid; Operation is after 15 days continuously, and polythene drum apposition growth microbial film also reaches steady state, ammonoxidation bacterium concentrating end of processing.
In step (2), the bacterial classification of ammonia oxidation bacteria inoculation liquid is all taken from the ammonia oxidation bacteria needing in micro-polluted water to be processed.
Ammonia oxidation bacteria after enrichment of the present invention is applied in the former water water pipe or water technology of micro-polluted water, also can be applicable to the basic test research to ammonia oxidation bacteria in micro-polluted water.
The present invention has the following advantages:
1. these apparatus and method are utilized the ammonia oxidation bacteria nutrient solution that organic carbon concentration is lower, and in maintain process, are suitable for the nutritional condition of ammonia oxidation bacteria growth, can make ammonoxidation bacterium concentrating cultivate, and heterotrophic bacterium growth is subject to obvious inhibition, and the ammonia oxidation bacteria concentration of enrichment is high.
The tap water of preparation ammonia oxidation bacteria nutrient solution take from need to the water factory or testing laboratory of this ammonia oxidation bacteria bacterial classification in, the bacterial classification of ammonia oxidation bacteria inoculation liquid take from need to the micro-polluted water of this ammonia oxidation bacteria bacterial classification in, can guarantee to obtain the bacterial classification adapting with water quality environment, there is wide spectrum suitability.
3. this enriching apparatus and method can complete whole enrichment process in 27 days, and enrichment time is short, simple to operate.
4. this ammonoxidation bacterium concentrating device can make the ammonia oxidation bacteria of enrichment be wrapped in microbial film, can be made into after treatment ammonia oxidation bacteria suspension inoculates, the problem of the high-concentration nutrient matter of bringing while avoiding directly inoculating ammonia oxidation bacteria inoculation liquid, has wide spectrum suitability.
Accompanying drawing explanation
Fig. 1 is a kind of schematic diagram that is applicable to the ammonoxidation bacterium concentrating device of micro-polluted water purification of the present invention.
Fig. 2 is the schematic diagram that ammonoxidation bacterium concentrating reactor water outlet ammonia nitrogen concentration in enrichment process of the present invention, nitrite concentration change with enrichment time.
Fig. 3 is that this embodiment 1 is inoculated into ammonia oxidation bacteria in the former water water delivery simulation pipeline of water purification plant, the schematic diagram that former water water delivery simulation pipeline changes with inoculation time the removal effect of ammonia nitrogen.
Fig. 4 is in the membrane cisterna of this embodiment 2 hyperfiltration membrane assembly of ammonia oxidation bacteria being inoculated into water purification plant, the schematic diagram that hyperfiltration membrane assembly changes with inoculation time the removal effect of ammonia nitrogen.
Fig. 5 is that this embodiment 3 is inoculated into ammonia oxidation bacteria in static micro-polluted water, and in water, ammonia oxidation bacteria exists the schematic diagram that level changes with inoculation time.
Wherein, A-ammonia oxidation bacteria nutritional control system, 1-ammonoxidation bacterium concentrating reactor, 2-ammonia oxidation bacteria nutrient solution holding tank, 3-motor, 4-rotor, 5-cross frame, 6-polythene drum, 7-intake pump, the rising pipe of 8-ammonoxidation bacterium concentrating reactor, the blow-down pipe of 9-ammonoxidation bacterium concentrating reactor, 10-water inlet pipe, the blow-down pipe of 11-ammonia oxidation bacteria nutrient solution holding tank, 12-controller, 13-dissolved oxygen meter, 14-dissolved oxygen electrode, 15-pH determinator, 16-pH electrode, the online ammonia nitrogen determination instrument of 17-, 18-air compressor, 19-adds alkali pump, 20-adds alkali tank, 21-adds ammonia pump, 22-ammonification tank, 23-aeration head, 24-input port, 25-output port, 26-electromagnetic relay.
Embodiment
Below in conjunction with accompanying drawing, by embodiment, the present invention is elaborated.
The ammonoxidation bacterium concentrating reactor 1 that the present invention adopts is radius 0.13m, the cylinder of high 0.25m, the peripheral thick polythene drum of 0.3cm that adopts, the polythene drum 6 that built-in 0.1cm is thick, its useful volume is 13L, top reserving hole, is used for placing the water inlet pipe of dissolved oxygen electrode 14, pH electrode 16, online ammonia nitrogen determination instrument, the water inlet pipe 10 of motor 3 and ammonia oxidation bacteria reactor; Ammonoxidation bacterium concentrating reactor 1 centre bit installs rotor 4, the cross frame that is parallel to bottom surface is respectively established in ammonoxidation bacterium concentrating reactor 1 top and bottom, rotor 4 is connected with the cross frame 5 at motor 3, ammonoxidation bacterium concentrating reactor two ends Shang Xia 1, polythene drum 6 is fixed on the vertical direction of two ends cross frame 5, rotor 4 rotates under the driving of direct-current machine 3, drives the rotation of polythene drum 6.Generation of interfaces shearing force between polythene drum 6 and water body, is conducive to biomembranous quick adhesion; The present invention arranges 5 cylinders of take the coaxial different diameter that rotor 4 is axle, and the impact that polyethylene brings to water quality is less, and both sides all can apposition growth microbial film, has increased greatly biomembranous biofilm area, and the Biofilm Colonization total area is 1.5m
2; Ammonoxidation bacterium concentrating reactor 1 bottom sets out water pipe 8 and blow-down pipe 9, and rising pipe 8 water exit positions approach reactor head, adopts the water outlet of overflow mode.
The dissolved oxygen electrode 14 that the present invention adopts is fluorescence dissolved oxygen electrode, the interfering factors that not faced by traditional embrane method dissolved oxygen electrode affects, without changing membrane module and filling liquid, without electrode is carried out to polishing, the dissolved oxygen meter 13 that the present invention adopts adopts fluorescence spectrometry dissolved oxygen, single point correction in air, precision is high, response fast, be not subject to ammonia nitrogen in water, vitriol, muriatic impact, it is 0～20mg/L that dissolved oxygen meter 13 can be surveyed dissolved oxygen concentration value scope, and corresponding analog output value is 0～3000mV; The pH electrode 16 that the present invention adopts is glass electrode, built-in reference electrode, temperature compensating electrode, the pH determinator 15 that the present invention adopts has automatic salinity compensation and pressure compensation, built-in endpoint and terminal technique of display, reading accuracy and time of response have been optimized, it is 0～14 that pH determinator 15 can be surveyed pH value scope, and corresponding analog output value is 4～20mA; The online ammonia nitrogen instrument 17 that the present invention adopts utilizes Ammonia Gas Sensor Electrode Method to measure ammonia nitrogen concentration, lowest detectable limit is required to be 0.02mg/L, there is automatic cleaning and automatic Calibration function, measure interval 5-120min adjustable, ammonia nitrogen sensor consists of jointly potassium ion-selective electrode, pH electrode and temperature electrode, and the value of recoverable, compensation ammonia nitrogen, further improves the accuracy of measuring, it is 0～14mg/L that online ammonia nitrogen determination instrument 17 can be surveyed ammonia nitrogen concentration value scope, and corresponding analog output value is 0～4000mV; The motor 3 that the present invention adopts is adjustable speed motor, and its speed range is: 10r/min-1000r/min, and rotating speed of the present invention is set to 150r/min; In the present invention, ammonoxidation bacterium concentrating reactor 1 water inlet, ammonification tank 22 and add 20 water outlets of alkali tank and all adopt peristaltic pump water intake mode, the speed range of peristaltic pump: 0.1-100rpm, rotating is reversible, move, stop being connected with corresponding electromagnetic relay 26 by external interface, the operation that utilizes electromagnetic relay 26 to realize pump, intake pump 7 water flows of the present invention are set to constant rate 110mL/min, add alkali pump 19 water flows and be set as constant rate 2mL/min, add ammonia pump 21 water flows and be set as constant rate 6mL/min.
The ammonia oxidation bacteria nutrient solution holding tank 2 that the present invention adopts is radius 0.26m, the cylinder plastic tank of high 0.6m, and useful volume is 120L, with cover, and top reserving hole is used for placing aeration tube, adds alkali pipe and ammonification pipe, and bottom arranges blow-down pipe 11.Ammonia oxidation bacteria nutrient solution holding tank 2 outer walls are pasted black plastic bag with lucifuge.The air compressor 18 top air drafts that the present invention adopts, avoid hot gas to reflux, and operation noise is low, moves, stops to be connected with corresponding rly. 26 by external interface.
Use the chlorine bleach liquor of 10mg/L to ammonoxidation bacterium concentrating reactor and ammonia oxidation bacteria nutrient solution holding tank sterilizing 24h, then with tap water and the distilled water of sterilizing, ammonoxidation bacterium concentrating reactor and ammonia oxidation bacteria nutrient solution holding tank are cleaned up, to reduce the heterotrophic bacterium in ammonoxidation bacterium concentrating reactor and ammonia oxidation bacteria nutrient solution holding tank.
Configuration ammonia oxidation bacteria nutrient solution, the preparation of the ammonia oxidation bacteria nutrient solution that the present invention adopts consists of: (NH
4)
2sO
4: 0.4g/L, NaCl:0.4g/L, MgSO
47H
2o:0.1g/L, K
2hPO
4: 0.2g/L, FeSO
47H
2o:0.1g/L, CaCO
3: 0.2g/L, is transferred to 8.0 by pH value, trace element solution: 0.2mL/L, 0.1% is phenol red: 0.2mL/L.Trace element solution composition is as follows: EDTA:2g/L, ZnSO
47H
2o:10g/L, CuSO
45H
2o:0.4g/L, Co (NO
3)
26H
2o:0.2g/L, Na
2b
4o
710H
2o:0.2g/L, adopts tap water preparation.
Prepare ammonia oxidation bacteria inoculation liquid: first detect the ammonia oxidation bacteria containing charcoal absorption in the ultra-filtration membrane assembled unit of gac that influent ammonium concentration in pending micro-polluted water is greater than 1mg/L, then get 10mL and put into ultrasonic oscillator containing the turbid solution of gac, ultrasonic frequency 40KHz, vibrate 20 minutes, get supernatant liquor, in supernatant liquor, ammonia oxidation bacteria concentration is 10 after testing
2mPN/mL, then by supernatant liquor, the volume ratio with 1:6 is inoculated in the ammonia oxidation bacteria nutrient solution of sterilizing, puts into 28 ° of incubator concussions and cultivates, and rotating speed is 150r/min, every day hydrogen make-up sodium oxide and ammonium chloride, make that pH value is 8.0, ammonia nitrogen concentration is 2mg/L, take 3 days as a generation, the volume ratio with 1:3 after a culture finishes proceeds to fresh ammonia oxidation bacteria nutrient solution, the rest may be inferred, needed altogether for 4 generations, so that ammonia oxidation bacteria enlarged culturing suppresses the growth of heterotrophic bacterium simultaneously.Ammonia oxidation bacteria adopts 3 pipe MPN dilutions to cultivate counting process and measures, and heterotrophic bacterium adopts R
ra colony counting method is measured.The quantity that records ammonia oxidation bacteria in inoculation liquid after cultivation finishes is 4.5x10
4mPN/mL, the quantity of heterotrophic bacterium is 0.9x10
3cFU/mL.
By above-mentioned steps, prepare ammonia oxidation bacteria nutrient solution and be injected into ammonia oxidation bacteria nutrient solution holding tank, while injecting ammonia oxidation bacteria nutrient solution to ammonia oxidation bacteria nutrient solution holding tank first, ammonia oxidation bacteria inoculation liquid and the volume ratio of step ammonia oxidation bacteria nutrient solution with 1:10 should be inoculated into ammonia oxidation bacteria in ammonia oxidation bacteria nutrient solution.The configuration ammonium chloride solution of 1g/L and the sodium hydroxide solution of 10g/L are also injected into respectively ammonification tank and add alkali tank, then start intake pump ammonia oxidation bacteria nutrient solution in ammonia oxidation bacteria nutrient solution holding tank is introduced to ammonoxidation bacterium concentrating reactor, open motor, air compressor, controller, add alkali pump, add ammonia pump, dissolved oxygen meter, online ammonia nitrogen determination instrument, pH determinator.
Enrichment process starts, apposition growth microbial film gradually on the polythene drum in ammonoxidation bacterium concentrating reactor.Dissolved oxygen concentration value, pH value, ammonia nitrogen concentration value in dissolved oxygen meter, pH determinator and online ammonia nitrogen determination instrument on-line monitoring ammonoxidation bacterium concentrating reactor is also adjusted to target zone in real time.Constantly supplement and add liquid in alkali tank, ammonification tank, ammonia oxidation bacteria nutrient solution holding tank during this time, to guarantee adding continuously of liquid.Operation is after 15 days continuously, and polythene drum apposition growth microbial film also reaches steady state, ammonoxidation bacterium concentrating end of processing.
Fig. 2 is the ammonia nitrogen concentration of ammonoxidation bacterium concentrating reactor water outlet in enrichment process of the present invention, the curve synoptic diagram that nitrite concentration changes with enrichment time, as can be seen from Figure 2, growth along with enrichment time, the ammonia nitrogen concentration of water outlet reduces gradually, nitrite nitrogen concentration first increases rear reduction, visible in ammonoxidation bacterium concentrating reactor, ammonia nitrogen is oxidized to nitrite nitrogen by ammonia oxidation bacteria, and then nitrite is oxidized to nitrate by nitrate bacterium.Within the 1st day, ammonia nitrogen concentration is lower, and this may be because inoculated ammonia oxidation bacteria in ammonia oxidation bacteria nutrient solution on the 1st day, the ammonia oxidation bacteria quantity that enters ammonia oxidation bacteria reactor is increased, thereby strengthened the Degradation to ammonia nitrogen.Along with the growth of enrichment time, ammonia nitrogen concentration reduces gradually, and after the 13rd day, ammonia nitrogen concentration can be stabilized in below 0.08mg/L.In enrichment, after 14 days, nitrite nitrogen can be stabilized in below 0.05mg/L.In visible ammonoxidation bacterium concentrating reactor, Dissolved Oxygen concentration Control is at 5～7mg/L, pH value is controlled at 8.0～8.5, ammonia nitrogen concentration is controlled within the scope of 4～8mg/L, can realize the efficiently concentrating of ammonia oxidation bacteria in microbial film, and the quantity of stablizing ammonia oxidation bacteria in artifact film is 10
4mPN/cm
2, total heterotrophic bacteria count is 1.8x10
3cFU/cm
2.
The ammonia oxidation bacteria of this apparatus and method enrichment can strengthen the denitrification effect of micro-polluted water is illustrated by embodiment 1, embodiment 2, and embodiment 1 and embodiment 2 are inoculated into ammonia oxidation bacteria in the former water water delivery simulation pipeline and ultra-filtration membrane membrane cisterna of micro-polluted water.At the 0h of inoculation, 2h, 8h, 24h, 36h, 48h measures the ammonia nitrogen concentration of simulation pipeline, ultra-filtration membrane membrane cisterna Inlet and outlet water, and indicates denitrification effect with ammonia nitrogen removal frank.Former water water delivery simulation pipeline and ultra-filtration membrane film are to the removal effect of ammonia nitrogen with the variation of inoculation time as shown in Figure 3 and Figure 4.
The ammonia oxidation bacteria of this enriching apparatus and method enrichment can be inoculated in micro-polluted water and keep certain biological activity, to increase the ammonia oxidation bacteria quantity in micro-polluted water, basic test research for micro-polluted water ammonia oxidation bacteria, by embodiment 3, illustrate, ammonia oxidation bacteria is inoculated in tap water, and ammonia oxidation bacteria quantity with the variation of inoculation time as shown in Figure 5.
Embodiment 1:
Former water water delivery simulation pipeline adopts polyethylene tube, and the internal-and external diameter of polyethylene tube is respectively 20mm, 24mm, and length of pipe section is 15m, and flow velocity is 1.0m/s, adopts circulating water flow mode, by changing the water circulation time, changes hydraulic detention time.The mean concns of former water water delivery simulation pipeline influent ammonia nitrogen is 2.5mg/L, and average water temperature is 25 ° of C, and average dissolved oxygen concentration is 4.9mg/L, and mean ph value is 7.3, and when hydraulic detention time is 1h, former water water delivery simulation pipeline is 53% to the average removal rate of ammonia nitrogen.Press the people such as Zhou Lingling (Zhou Lingling, Zhang Yongji, Yang Yanling, Li Guibai; A kind of measuring method of amount of colonies of adhesivity heterotrophic bacteria; ZL200810064710.5) to adhering to the treatment process of heterotrophic bacterium, the present invention is attached in polythene drum to the ammonia oxidation bacteria in microbial film and is prepared into ammonia oxidation bacteria suspension and inoculates, ammonia oxidation bacteria microbial film scraping area is pressed the long-pending (cm of inner surface of tube wall
2) 10% carry out scraping, after the 1h of interval, inoculation again, and using this as inoculation time starting point 0h.After inoculation, former water simulation pipeline keeps continuous circulation running status, and the 0h inoculating, 2h, 8h, 24h, 36h, 48h measures the ammonia nitrogen concentration of former water water delivery simulation pipeline Inlet and outlet water, and indicates the removal effect to ammonia nitrogen with ammonia nitrogen removal frank, and former water water delivery simulation pipeline to the removal effect of ammonia nitrogen as shown in Figure 3.As can be seen from Figure 3 along with the increase of inoculation time, ammonia nitrogen removal frank increases gradually, and when hydraulic detention time is 8h, ammonia nitrogen removal frank is 88%, is finally stabilized in more than 90%.It can be said that bright former water water delivery simulates ducted ammonia oxidation bacteria and have larger biological activity, at former water water delivery simulation pipe interior, completed nitrosification.Visible by the ammonia oxidation bacteria of inoculation apparatus of the present invention and method enrichment, former water water delivery simulation pipeline has obviously improved biologica denitrification, can realize the effective removal to ammonia nitrogen in micro-polluted water.
Embodiment 2:
Hyperfiltration membrane assembly adopts the middle control tunica fibrosa of polyvinylidene difluoride (PVDF) material, normal pore size is 0.01um, film silk internal-and external diameter is respectively 0.85um, 1.45um, hydraulic detention time is 0.5h, hyperfiltration membrane assembly influent ammonia nitrogen mean concns is 1.2mg/L, and average water temperature is 25 ° of C, and average dissolved oxygen concentration is 3.7mg/L, mean ph value is 7.2, and during steady running, this hyperfiltration membrane assembly is 23% to the average removal rate of ammonia nitrogen.Press the people such as Zhou Lingling (Zhou Lingling, Zhang Yongji, Yang Yanling, Li Guibai; A kind of measuring method of amount of colonies of adhesivity heterotrophic bacteria; ZL200810064710.5) to adhering to the treatment process of heterotrophic bacterium, the present invention is attached in polythene drum to the ammonia oxidation bacteria in microbial film and is prepared into ammonia oxidation bacteria suspension and inoculates, ammonia oxidation bacteria microbial film scraping area is pressed the long-pending (cm of inner surface of tube wall
2) 10% carry out scraping, after the 0.5h of interval, inoculation again, and using this as inoculation time starting point 0h.At the 0h of inoculation, 2h, 8h, 24h, 36h, 48h measures the Inlet and outlet water ammonia nitrogen concentration of ultra-filtration membrane membrane cisterna, and indicates the removal effect of hyperfiltration membrane assembly to ammonia nitrogen with ammonia nitrogen removal frank, and hyperfiltration membrane assembly is to the removal effect of ammonia nitrogen as shown in Figure 4.As can be seen from Figure 4 along with the increase of inoculation time, ammonia nitrogen removal frank increases gradually, finally tends towards stability, and when hydraulic detention time is 24h, ammonia nitrogen removal frank is 83%.It can be said that the ammonia oxidation bacteria in bright ultra-filtration membrane membrane cisterna has larger biological activity, has completed nitrosification in hyperfiltration membrane assembly inside.Visible by the ammonia oxidation bacteria of inoculation apparatus of the present invention and method enrichment, hyperfiltration membrane assembly has obviously improved biologica denitrification, can realize the effective removal to ammonia nitrogen in micro-polluted water.
Embodiment 3:
Tap water is taken from laboratory, and after measured, in tap water, ammonia oxidation bacteria quantity is 45MPN/mL.By tap water airing dechlorination, by the people such as Zhou Lingling (Zhou Lingling, Zhang Yongji, Yang Yanling, Li Guibai; A kind of measuring method of amount of colonies of adhesivity heterotrophic bacteria; ZL200810064710.5), to adhering to the treatment process of heterotrophic bacterium, the present invention is attached in polythene drum to the ammonia oxidation bacteria in microbial film and is prepared into ammonia oxidation bacteria suspension and inoculates, ammonia oxidation bacteria microbial film scraping area value (cm
2) by 10% of tap water volume cost (L), carry out scraping.Dark culturing after inoculation, and at the 0h of inoculation, 6h, 12h, 24h, 48h, 72h measures the ammonia oxidation bacteria quantity in tap water, and the ammonia oxidation bacteria quantity in tap water is with the variation of inoculation time as shown in Figure 5.As can be seen from Figure 5, after inoculation ammonia oxidation bacteria, in tap water, the quantity of ammonia oxidation bacteria obviously increases, and along with the growth of inoculation time, ammonia oxidation bacteria recovers its activity gradually, and tend towards stability at 24h, ammonia oxidation bacteria quantity reaches 1500MPN/mL, and after inoculation 48h, ammonia oxidation bacteria quantity starts to reduce, this may be the minimizing due to the nutritive substance such as ammonia nitrogen, dissolved oxygen in tap water, has affected the activity of ammonia oxidation bacteria.Visible by the ammonia oxidation bacteria of inoculation apparatus of the present invention and method enrichment, the ammonia oxidation bacteria quantity in tap water obviously improves, and its biological activity can maintain 24h, can be applicable to the basic test research to ammonia oxidation bacteria in micro-polluted water.
Claims (4)
1. a method that is applicable to the ammonoxidation bacterium concentrating that micro-polluted water purifies, it is characterized in that, the device of employing is mainly comprised of ammonoxidation bacterium concentrating reactor (1), ammonia oxidation bacteria nutrient solution holding tank (2) and ammonia oxidation bacteria nutritional control system (A);
Ammonoxidation bacterium concentrating reactor (1) top is provided with motor (3), inside is provided with rotor (4), rotor (4) top and bottom are provided with the cross bar (5) of parallel bottom surface, between two ten supports (5), vertical direction is fixed with coaxial polythene drum (6), rotor (4) can rotate under the driving of motor (3), and drive cross bar (5) to rotate the rotation that realizes polythene drum (6), the rotation of polythene drum (6) is conducive to biomembranously adhere to fast, ammonoxidation bacterium concentrating reactor (1) is connected with ammonia oxidation bacteria nutrient solution holding tank (2) via intake pump (7), can realize the feed liquor of ammonoxidation bacterium concentrating reactor (1), ammonoxidation bacterium concentrating reactor (1) top sets out water pipe (8), adopt the draining of overflow mode, bottom is provided with blow-down pipe (9),
Ammonia oxidation bacteria nutrient solution holding tank (2) is for depositing ammonia oxidation bacteria nutrient solution, ammonia oxidation bacteria nutrient solution holding tank (2) is communicated with ammonoxidation bacterium concentrating reactor (1) by water inlet pipe (10) via intake pump (7), it is the water feed apparatus of ammonoxidation bacterium concentrating reactor (1), intake pump (7) is set on water inlet pipe (10), and ammonia oxidation bacteria nutrient solution holding tank (2) bottom is provided with blow-down pipe (11);
Ammonia oxidation bacteria nutritional control system (A) comprises controller (12), dissolved oxygen meter (13), dissolved oxygen electrode (14), pH determinator (15), pH electrode (16), online ammonia nitrogen determination instrument (17), air compressor (18), add alkali pump (19) and add alkali tank (20), add ammonia pump (21) and ammonification tank (22), wherein adding alkali tank (20) is connected with ammonia oxidation bacteria nutrient solution holding tank (2) by adding alkali pump (19), ammonification tank (22) is connected with ammonia oxidation bacteria nutrient solution holding tank (2) by adding ammonia pump (21), air compressor (18) is connected with ammonia oxidation bacteria nutrient solution holding tank (2) by aeration tube, aeration head (23) is placed in ammonia oxidation bacteria nutrient solution holding tank (2) bottom, the input port (24) of controller (12) is connected with ammonoxidation bacterium concentrating reactor (1) with dissolved oxygen electrode (14), pH electrode (16) by dissolved oxygen meter (13), pH determinator (15) respectively, and the input port of same Time Controller (12) is also connected with ammonoxidation bacterium concentrating reactor (1) by online ammonia nitrogen determination instrument (17), the output port (25) of controller (12) by electromagnetic relay (26) respectively with air compressor (18), add alkali pump (19), add ammonia pump (21) and be connected,
Comprise the following steps:
(1) adopt tap water preparation ammonia oxidation bacteria nutrient solution;
(2) prepare ammonia oxidation bacteria inoculation liquid, the gac turbid solution that contains ammonia oxidation bacteria is prepared into ammonia oxidation bacteria suspension, get its supernatant liquor, supernatant liquor is inoculated into the volume ratio of 1:6 in the ammonia oxidation bacteria nutrient solution of step (1) of sterilizing, putting into 28 ℃ of incubator concussions cultivates, rotating speed is 150r/min, every day hydrogen make-up sodium oxide and ammonium chloride, making pH value is 8.0, ammonia nitrogen concentration is 2mg/L, take 3 days as a generation, after one culture finishes, the volume ratio with 1:3 proceeds in step (1) the ammonia oxidation bacteria nutrient solution to sterilizing again, the like, needed altogether for 4 generations, so that ammonia oxidation bacteria enlarged culturing, suppress the growth of heterotrophic bacterium simultaneously,
(3) preparation ammonium chloride solution and sodium hydroxide solution are also injected into respectively ammonification tank and add alkali tank, the ammonia oxidation bacteria nutrient solution of step (1) preparation is injected into ammonia oxidation bacteria nutrient solution holding tank, while injecting first, should the ammonia oxidation bacteria nutrient solution of the ammonia oxidation bacteria inoculation liquid of step (2) and step (1) be inoculated into ammonia oxidation bacteria in the ammonia oxidation bacteria nutrient solution of step (1) with the volume ratio of 1:10;
(4) start intake pump the ammonia oxidation bacteria nutrient solution in ammonia oxidation bacteria nutrient solution holding tank is introduced to ammonoxidation bacterium concentrating reactor, then open ammonoxidation bacterium concentrating reactor motor, air compressor, controller, add alkali pump, add ammonia pump, dissolved oxygen meter, pH determinator and online ammonia nitrogen determination instrument, keeping maintaining dissolved oxygen concentration in ammonoxidation bacterium concentrating reactor is that 5～7mg/L, ammonia nitrogen concentration are that 4～8mg/L, pH value are 8.0～8.5, and enrichment process starts;
(5) after enrichment process starts, apposition growth microbial film gradually on the polythene drum in ammonoxidation bacterium concentrating reactor;
(6), from step (5), constantly supplement and add liquid in alkali tank, ammonification tank, ammonia oxidation bacteria nutrient solution holding tank, to guarantee adding continuously of liquid; Operation is after 15 days continuously, and polythene drum apposition growth microbial film also reaches steady state, ammonoxidation bacterium concentrating end of processing.
2. according to the method for claim 1, it is characterized in that, the tap water of step (1) take from need to the water factory or testing laboratory of this ammonia oxidation bacteria bacterial classification in, the bacterial classification of the ammonia oxidation bacteria inoculation liquid in step (2) is taken from the ammonia oxidation bacteria needing in pending micro-polluted water.
3. according to the ammonia oxidation bacteria of the method enrichment of claim 1 or 2, be applied to the processing to micro-polluted water.
4. according to the ammonia oxidation bacteria of the method enrichment of claim 1 or 2, be applied in the former water water pipe or water technology of micro-polluted water.
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| CN105084552A (en) * | 2015-07-31 | 2015-11-25 | 北京师范大学 | Method for enhancing aerobiotic ammonia oxidizing bacterium gathering through N-acylated homoserine lactones |
| CN106399201A (en) * | 2016-11-10 | 2017-02-15 | 天津工业大学 | High-efficiency enrichment culture method of Pseudomonas aeruginosa |
| CN109592792A (en) * | 2017-09-30 | 2019-04-09 | 温州大学 | A kind of sewage-treatment plant and processing method suitable for eutrophication water |
| CN108395005B (en) * | 2018-05-11 | 2024-03-22 | 苏州北控环保科技有限公司 | Intelligent biomembrane river course treatment system |
| CN110776101A (en) * | 2019-11-22 | 2020-02-11 | 中国市政工程华北设计研究总院有限公司 | Device for treating urban sewage by utilizing partial nitrosation-anaerobic ammonia oxidation process and method used by device |
| CN114804341A (en) * | 2022-04-29 | 2022-07-29 | 华北电力大学 | Device and method for rapidly enriching complete ammonia oxidizing bacteria from activated sludge and application |
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| CN101289688B (en) * | 2008-06-12 | 2011-08-17 | 哈尔滨工业大学 | Measurement method for amount of colonies of adhesivity heterotrophic bacteria |
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