CN109943479A - A kind of automatic enrichment culture device and method of resistance to bacterium with high salt - Google Patents
A kind of automatic enrichment culture device and method of resistance to bacterium with high salt Download PDFInfo
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- CN109943479A CN109943479A CN201711384701.XA CN201711384701A CN109943479A CN 109943479 A CN109943479 A CN 109943479A CN 201711384701 A CN201711384701 A CN 201711384701A CN 109943479 A CN109943479 A CN 109943479A
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Abstract
The invention discloses a kind of automatic enrichment culture devices of resistance to bacterium with high salt, including several enrichment culture casees, feed liquid blending tank, acid storage tank, liquid alkaline storage tank, blower, temperature sensor, dissolved oxygen meter, pH meter, transmissometer, liquidometer, TDS on-line computing model and processor；The invention also discloses a kind of automatic Enrichment culture methods of resistance to bacterium with high salt, including process one: culture medium is prepared；Process two: resistance to bacterium enrichment culture with high salt.Present apparatus structure is simple, has the advantages such as occupied area is small, stable, automatic operation is strong, continuity is good, control is accurate.Utilize the resistance to bacterium with high salt of present apparatus culture, mainly pass through automatic control system, realize culture medium add, the control that thallus adds ratio etc. in thalli growth environmental Kuznets Curves, thalli growth condition monitoring and system, it fundamentally solves coal chemical industry high-salt wastewater to be difficult to adapt to using thallus when biochemical treatment, it is difficult to be enriched with, the problems such as Biochemical Treatment difference.
Description
Technical field:
The present invention relates to a kind of automatic enrichment culture device and methods of resistance to bacterium with high salt, belong to environmentally friendly water treatment field and oneself
The combination in dynamicization field.
Background technique:
The high slat-containing wastewater of coal chemical industry is mainly derived from the draining of the circulation in production process, chemical water station row
Water, washes, process system condensate liquid have complicated water-quality constituents, salt content height, hardness height, pollutant kind more, contain
The features such as hardly degraded organic substance.In recent years, as the whole nation especially the Northwest's presentation water resources shortage, water pollution are serious and water
The water environmental problems outstanding such as environmental carrying capacity is extremely low, it is maximum that environmental protection administration requires coal chemical industry enterprises to carry out Halite water
Reuse, environmentally sensitive area more propose wastewater zero discharge requirement.
Biochemical treatment is to rely on biologic treating technique, and environment sensitive substance, which is degraded by microorganisms, to be reached environment and allow to arrange
Put standard, there are at low cost, high efficiency and the advantages such as without secondary pollution, obtained traditional wastewater treatment industry expert approval and
It is widely applied.However, directly resulting in common micro-organisms cell dehydration, the chlorine of high concentration due to salt content height in coal chemical industrial waste water
Ion pair microorganism also has certain toxic action, so that microorganism can not survive, can not carry out organic matter, ammonia nitrogen and nitrate anion
The biochemical degradation of equal substances, causes fouling membrane in entire Zero discharging system operational process serious, the easy fouling of equipment, produces water and does not conform to
Lattice, evaporative crystallization are difficult to stable operation and seriously affect crystal salt quality etc..Therefore, enrichment culture is tolerable high containing salt chemical engineering
The strain of waste water is most important.
Summary of the invention:
To solve the problems, such as above-mentioned resistance to bacterium enrichment culture process with high salt, the first purpose of this invention is to provide
A kind of automatic enrichment culture device of resistance to bacterium with high salt.
It is main by controlling automatically a second object of the present invention is to provide a kind of automatic Enrichment culture method of resistance to bacterium with high salt
System processed realizes that culture medium adds, thallus adds ratio in thalli growth environmental Kuznets Curves, thalli growth condition monitoring and system
Deng control, fundamentally solve coal chemical industry high-salt wastewater thallus enrichment culture process present in problem.
The first purpose of this invention has following scheme to implement: a kind of automatic enrichment culture device of resistance to bacterium with high salt, packet
Include several enrichment culture casees, feed liquid blending tank, acid storage tank, liquid alkaline storage tank, blower, heater, temperature sensor, dissolved oxygen meter, pH
Meter, transmissometer, liquidometer, TDS on-line computing model and processor；
It is equipped with fluorescent lamp in each enrichment culture case, is communicated in the side wall upper part of each enrichment culture case
Exhaust pipe is communicated with evacuated tube in the lower sidewall of each enrichment culture case, and exhaust-valve is equipped in the evacuated tube；?
Bottom in each enrichment culture case is equipped with aeration tube, and aeration valve and aeration head, every institute are equipped in the aeration tube
Aeration tube is stated to connect with the blower and pipeline；The heater, the temperature are equipped in each enrichment culture case
Spend sensor, the dissolved oxygen meter, the pH meter, the transmissometer, the liquidometer and the TDS on-line computing model；
The adjacent enrichment culture case is connected to by pipeline, is equipped with and is turned on the pipeline for being connected to the adjacent enrichment culture case
Liquid valve；
The outlet of the feed liquid blending tank is connected to the entrance of feed pump, the outlet of the feed pump respectively with it is each described
The import of enrichment culture case is connected to by pipeline, is equipped with charging on the pipeline for being connected to the feed pump and the enrichment culture case
Valve；
The outlet of the acid storage tank is connected to the entrance of sour metering pump, the outlet of the liquid alkaline storage tank and entering for alkali metering pump
The outlet of mouth connection, the acid metering pump and the alkali metering pump is connected with each enrichment culture case by pH regulation pipe respectively
It is logical, pH regulating valve is equipped in each pH regulation pipe；
The temperature sensor, the dissolved oxygen meter, the pH meter, the transmissometer, the liquidometer, the TDS are online
Monitor and the electromagnetic flowmeter are electrically connected with the input terminal of the processor, the output end of the processor respectively with institute
State feed pump, the inlet valve, the blower, the heater, the aeration valve, the exhaust-valve, the liquid valve, described of turning
Fluorescent lamp, the sour metering pump, the alkali metering pump and pH regulating valve electrical connection.
Further, the enrichment culture case is four.
Further, further include culture medium raw material tank and NaCl solution storage tank, the outlet of the culture medium raw material tank and
The outlet of the NaCl solution storage tank is connected to the entrance of the feed liquid blending tank by pipeline, and the culture base is being connected to
The pipeline of batch can and the feed liquid blending tank is equipped with culture medium control valve, is being connected to the NaCl solution storage tank and the feed liquid
The pipeline of blending tank is equipped with NaCl control valve, the culture medium control valve and the NaCl control valve with the processor
Output end electrical connection.
Further, it is equipped with culture medium blender in the culture medium raw material tank, is set in the NaCl solution storage tank
There is NaCl blender, the culture medium blender and the NaCl blender are electrically connected with the output end of the processor.
Further, further include hydraulic mixing pipe and return valve on the hydraulic mixing pipe and be set to described
Hydraulic mixing pump in evacuated tube, the hydraulic mixing pump and the return valve are electrically connected with the output end of the processor；
One end of the hydraulic mixing pipe is set in the enrichment culture case, the other end and the hydraulic mixing of the hydraulic mixing pipe
Evacuated tube connection between pump and the return valve.
It further, further include electromagnetic flowmeter, the electromagnetic flowmeter, which is set to, is connected to the blower and the aeration
On the pipeline of pipe.
Second object of the present invention is implemented by following technical solution: a kind of automatic Enrichment culture method of resistance to bacterium with high salt,
It comprises the following steps:
Process one: culture medium is prepared
Culture medium is mixed in feed liquid mixing tank in advance with NaCl, obtains the culture medium solution of appropriate salt content, then
Each enrichment culture case is sequentially added, the TDS of culture medium solution described in the adjacent enrichment culture case increases step by step, and adjacent institute
The TDS amplification of culture medium solution described in enrichment culture case is stated no more than 20000mg/L；Wherein, enrichment culture described in the first order
The TDS of culture medium solution described in case is 5000mg/L~10000mg/L；
Process two: resistance to bacterium enrichment culture with high salt
It adds gram-positive bacteria in the enrichment culture case described in the first order to be cultivated, to enrichment culture described in the first order
Bacteria concentration in case reaches 1010When cFU/ml or more, strain is in growth stationary phase, takes or enters to be enriched with described in the second level and trains
Feeding case continues enrichment culture；It repeats the above steps, obtains the resistance to bacterium with high salt for handling different salt content waste water；
In the process one: culture medium is prepared and the process two: in resistance to bacterium enrichment culture process with high salt, controlling each described
15-40 DEG C of temperature, pH 6.0-9.0, dissolved oxygen 0.03-0.2mmol/L in enrichment culture case.
Further, the pH of culture medium solution described in the pH meter real-time monitoring, feedback processor, control sour metering pump or
The opening and closing of alkali metering pump, and then the pH of the culture medium solution is effectively adjusted, it is specific:
When the pH of the culture medium solution reaches the setting value upper limit, the sour metering pump is opened, being pumped into HCl reduces pH；
When the pH of the culture medium solution reaches setting value lower limit, the alkali metering pump is opened, liquid alkaline is pumped into and improves pH.
Further, in the process two: in resistance to bacterium enrichment culture with high salt, culture medium described in the dissolved oxygen meter real-time monitoring
The dissolved oxygen amount of solution feeds back the processor, controls the opening and closing of the start and stop of blower with corresponding aeration valve.
Further, the temperature of culture medium solution described in the temperature sensor real-time monitoring, feeds back the processor, control
The opening and closing of heater or hydraulic mixing pump processed, adjusts the temperature of the culture medium solution.
Further, in the process two: in resistance to bacterium enrichment culture with high salt, since Facultative Halophiles consume culture medium, making described
The TDS of culture medium solution constantly rises, and the TDS of culture medium solution described in TDS on-line computing model real-time monitoring feeds back the processing
Device, when the TDS content of the culture medium solution is higher than the setting value upper limit, Primary culture base control valve, feed pump and corresponding
Inlet valve, when the TDS content of the culture medium solution reaches setting value lower limit, close the culture medium control valve, it is described into
Material pump and the corresponding inlet valve.
Further, temperature 25-35 DEG C is controlled in each enrichment culture case.
Further, dissolved oxygen 0.05-0.2mmol/L in each enrichment culture case is controlled.
Advantages of the present invention:
1, the present invention provides a kind of automatic enrichment culture device of resistance to bacterium with high salt, structure is simple, small with occupied area,
The stable, advantages such as automatic operation is strong, continuity is good, control is accurate.
2, the present invention also provides a kind of automatic Enrichment culture method of resistance to bacterium with high salt, mainly pass through automatic control system,
Realize culture medium add, the control of thalli growth environmental Kuznets Curves, thalli growth condition monitoring etc., selective enrichment obtains locating
The resistance to bacterium with high salt for managing different salt content waste water fundamentally solves existing coal chemical industry high-salt wastewater using thallus when biochemical treatment
It being difficult to adapt to, it is difficult to the problem of enrichment reduces the dirty stifled and fouling risk of system operation, reduces evaporation and crystallization system operating pressure,
The quality of crystal salt is improved simultaneously.
3, the present invention accurately measuring by in-line meters such as temperature sensor, pH meter, dissolved oxygen meters, and feedback processor,
The accurate control that thalli growth environment is realized by sour metering pump, alkali metering pump, blower, hydraulic mixing pump etc., effectively avoids
Occurs the offset of a certain index in enrichment process；And pass through transmissometer real-time monitoring thalli growth situation, stable, automation
Degree is high.
Detailed description of the invention:
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
It obtains other drawings based on these drawings.
Fig. 1 is the automatic enrichment culture apparatus structure schematic diagram of the resistance to bacterium with high salt of embodiment 1.
Fig. 2 is the control schematic diagram of embodiment 1.
In figure: culture medium raw material tank 1, NaCl solution storage tank 2, enrichment culture case 3, feed liquid blending tank 4, acid storage tank 5, liquid alkaline
Storage tank 6, blower 7, temperature sensor 8, dissolved oxygen meter 9, pH meter 10, transmissometer 11, liquidometer 12, TDS on-line computing model 13, processing
Device 14, culture medium blender 15, NaCl blender 16, culture medium control valve 17, NaCl control valve 18, fluorescent lamp 19, exhaust pipe
20, evacuated tube 21, exhaust-valve 22, aeration tube 23 is aerated valve 24, and aeration head 25, electromagnetic flowmeter 26 turns liquid valve 27, feed pump
28, inlet valve 29, sour metering pump 30, alkali metering pump 31, pH regulation pipe 32, pH regulating valve 33, hydraulic mixing pipe 34, hydraulic mixing
Pump 35, return valve 36, heater 37.
Specific embodiment:
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Embodiment 1:
A kind of automatic enrichment culture device of resistance to bacterium with high salt, including 2, four culture medium raw material tank 1, NaCl solution storage tank richnesses
Collect incubator 3, feed liquid blending tank 4, acid storage tank 5, liquid alkaline storage tank 6, blower 7, temperature sensor 8, dissolved oxygen meter 9, pH meter 10, turbidity
Instrument 11, liquidometer 12, TDS on-line computing model 13, heater 37 and processor 14；
It is equipped with culture medium blender 15 in culture medium raw material tank 1, NaCl blender is equipped in NaCl solution storage tank 2
16, the outlet of culture medium raw material tank 1 and the outlet of NaCl solution storage tank 2 are connected to the entrance of feed liquid blending tank 4 by pipeline,
Culture medium control valve 17 is equipped on the pipeline of connection culture medium raw material tank 1 and feed liquid blending tank 4, in connection NaCl solution storage tank
2 and feed liquid blending tank 4 pipeline be equipped with NaCl control valve 18,
It is equipped with fluorescent lamp 19 in each enrichment culture case 3, is communicated with exhaust in the side wall upper part of each enrichment culture case 3
Pipe 20 is communicated with evacuated tube 21 in the lower sidewall of each enrichment culture case 3, and exhaust-valve 22 is equipped in evacuated tube 21；
It further includes hydraulic mixing pipe 34 and return valve 36 on hydraulic mixing pipe 34 and is set in evacuated tube 21
Hydraulic mixing pump 35, one end of hydraulic mixing pipe 34 is set in enrichment culture case 3, the other end and waterpower of hydraulic mixing pipe 34
Evacuated tube 21 between mixing pump 35 and exhaust-valve 22 is connected to；
Bottom in each enrichment culture case 3 is equipped with aeration tube 23, and aeration valve 24 and aeration are equipped on aeration tube 23
First 25, every aeration tube 23 is connect with blower 7 by pipeline, and electromagnetism is equipped on the pipeline of connection blower 7 and aeration tube 23
Flowmeter 26；In each enrichment culture case 3 be equipped with temperature sensor 8, dissolved oxygen meter 9, pH meter 10, transmissometer 11, liquidometer 12,
TDS on-line computing model 13 and heater 37；
Adjacent enrichment culture case 3 is connected to by pipeline, is equipped on the pipeline for being connected to adjacent enrichment culture case 3 and is turned liquid valve
27；
The outlet of feed liquid blending tank 4 is connected to the entrance of feed pump 28, and the outlet of feed pump 28 is trained with each enrichment respectively
The import for supporting case 3 is connected to by pipeline, and inlet valve 29 is equipped on the pipeline of connection feed pump 28 and enrichment culture case 3；
The outlet of acid storage tank 5 is connected to the entrance of sour metering pump 30, the outlet of liquid alkaline storage tank 6 and the entrance of alkali metering pump 31
Connection, the outlet of sour metering pump 30 and alkali metering pump 31 is connected to each enrichment culture case 3 by pH regulation pipe 32 respectively, every
A pH regulation pipe 32 is equipped with pH regulating valve 33；
Temperature sensor 8, dissolved oxygen meter 9, pH meter 10, transmissometer 11, liquidometer 12, TDS on-line computing model 13 and electromagnetic current
Meter 26 is electrically connected with the input terminal of processor 14, and the output end of processor 14 is stirred with culture medium blender 15, NaCl respectively
Mix device 16, culture medium control valve 17, NaCl control valve 18, feed pump 28, inlet valve 29, blower 7, aeration valve 24, exhaust-valve 22,
Turn liquid valve, fluorescent lamp 19, sour metering pump 30, alkali metering pump 31, pH regulating valve 33 and heater 37, hydraulic mixing pump 35 and reflux
Valve 36 is electrically connected.
Embodiment 2:
Using a kind of method of the resistance to bacterium with high salt of automatic enrichment culture device enrichment culture of resistance to bacterium with high salt of embodiment 1,
Step 1: preparing.
Respectively in culture medium raw material tank 1, NaCl solution storage tank 2, acid storage tank 5, liquid alkaline storage tank 6 be added liquid medium,
NaCl solution, HCl solution, NaOH；The highest liquid level that each enrichment culture case 3 is arranged is the 80% of its volume, the turbidity upper limit is
1011CFU/ml, pH bound be respectively 9.0 and 6.0, temperature up and down be limited to 40 DEG C and 15 DEG C, dissolved oxygen amount up and down be limited to
The TDS setting value of 0.2mmol/L and 0.03mmol/L and first order enrichment culture case 3 is 10000mg/L, the second level is enriched with
Incubator 3TDS setting value is 20000mg/L, the TDS setting value of third level enrichment culture case 3 is 30000mg/L, the fourth stage is rich
Integrate the TDS setting value of incubator 3 as 50000mg/L.
Step 2: initialization.
Setting return valve 36 is normally opened first, and setting exhaust-valve 22 is normally closed；
Culture medium control valve 17 and NaCl control valve 18 are then opened, NaCl solution and training are realized in feed liquid blending tank 4
Support the culture medium solution that is mixed to get of base, wherein the TDS for controlling culture medium solution be the first order enrichment culture case 3 set
TDS, control culture medium solution volume be first order enrichment culture case 3 upper limit level needed for volume；Then first is opened
The inlet valve 29 of grade enrichment culture case 3, opens feed pump 28, feeds for first order enrichment culture case 3, completes first order enrichment training
Support the initialization of case 3；And so on, second level enrichment culture case 3, third level enrichment culture case 3 and fourth stage enrichment culture case 3
Initialization procedure it is identical as first order enrichment culture case 3, be only that TDS and the volume of enrichment culture liquid for corresponding enrichment training
Support the parameter of case 3；
Finally, starting hydraulic mixing pump 35, opens fluorescent lamp 19, initialization is completed.
Step 3: inoculation.
It is first turned on pH regulating valve 33, starts sour metering pump 30 or alkali metering pump 31, adjusts the pH of culture medium solution；It opens
It is aerated valve 24, starting blower 7 is aerated, and the dissolved oxygen amount of culture medium solution is adjusted；
It is then inoculated in first order enrichment culture case 3, strain screens to obtain using Baya ur, NeiMengGu city Linhe District
Facultative Halophiles bacteria suspension, the additional amount of bacteria suspension is the 3-5v% of culture medium solution in first order enrichment culture case 3, according to turbid
It spends instrument 11 and measures growth situation, when turbidity is greater than the set value, opening turns the 27 turns of liquid cultures of liquid valve, and turns under liquid measure is
The 3-5v% of culture medium solution in grade enrichment culture case 3.
When the turbidity that transmissometer 11 in fourth stage enrichment culture case 3 detects culture medium solution reaches requirement, enrichment culture
It completes.After the completion of enrichment culture, closing volume valve 36 opens exhaust-valve 22, and it is resistance to high salt to obtain finished product for starting hydraulic mixing pump 35
Bacterium.
The regulation of initialization procedure and pH, dissolved oxygen amount, temperature in the present invention is by program setting, it can be achieved that automatic control
System,
The pH of 10 real-time monitoring culture medium solution of pH meter, feedback processor 14 control sour metering pump 30 or alkali metering pump 31
Opening and closing, and then effectively adjust the pH of culture medium solution, it is specific:
As the pH of culture medium solution > 9.0, sour metering pump 30 is opened, being pumped into HCl reduces pH；
As the pH of culture medium solution < 6.0, alkali metering pump 31 is opened, liquid alkaline is pumped into and improves pH.
The dissolved oxygen amount of 9 real-time monitoring culture medium solution of dissolved oxygen meter, feedback processor 14, control blower 7 start and stop with it is corresponding
It is aerated the opening and closing of valve 24.
The temperature of 8 real-time monitoring culture medium solution of temperature sensor, feedback processor 14, controls heater 37 or waterpower is stirred
The opening and closing for mixing pump 35, realizes the adjustment of temperature.
In process two: in resistance to bacterium enrichment culture with high salt, since Facultative Halophiles consume culture medium, making the TDS of culture medium solution not
It is disconnected to rise, the TDS of 13 real-time monitoring culture medium solution of TDS on-line computing model, feedback processor 14, as the TDS of culture medium solution
When content is higher than the setting value upper limit, Primary culture base control valve 17, feed pump 28 and corresponding inlet valve 29, to culture medium solution
TDS content when reaching setting value lower limit, close culture medium control valve 17, feed pump 28 and corresponding inlet valve 29.
Test analysis is carried out to the resistance to bacterium with high salt that 2 enrichment culture of embodiment obtains,
Firstly, measurement thalli growth situation.Take the resistance to bacterium with high salt of finished product as sample, using the concentration of bacterial suspension one
Determine to be inversely proportional in range with light transmittance, the principle directly proportional to optical density, using ultraviolet-visible spectrophotometer, in wavelength
The optical density OD=653 for measuring culture solution at 642nm with colorimetric cylinder timing, under the conditions of illustrating this kind, the life of the resistance to bacterium with high salt of finished product
Length is in order；
Secondly, being in as a result bluish violet, being determined as gram using Gram's stain bacterium with high salt resistance to finished product as sample
Positive bacteria.
Again, the resistance to bacterium sample with high salt of finished product carries out high saliferous wastewater from chemical industry organic matter removal test.By the resistance to bacterium with high salt of finished product
Into high saliferous wastewater from chemical industry to be processed, the high saliferous wastewater from chemical industry total dissolved solid TDS is sample inoculation
46823mg/L, measures the initial COD value of high saliferous wastewater from chemical industry respectively and COD value is respectively 760mg/ after degradation in 8 hours
L and 120mg/L, degradation effect of organic compound is high, up to 84.2%；It can be considered that resistance to bacterium with high salt is enriched with successfully, bioactivity is high.
Automatic enrichment culture device by resistance to bacterium with high salt and the side using the resistance to bacterium with high salt of the automatic enrichment culture of the device
Method is, it can be achieved that culture medium adds being precisely controlled for control, thalli growth environmental Kuznets Curves, thalli growth condition monitoring etc., realization choosing
The enrichment culture of selecting property obtains different degrees of resistance to bacterium with high salt, and Facultative Halophiles growth conditions are good, bioactivity is high, solve tradition
The problem of microorganism can not survive under hypersaline environment.
The above is merely preferred embodiments of the present invention, be not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (13)
1. a kind of automatic enrichment culture device of resistance to bacterium with high salt, which is characterized in that it includes several enrichment culture casees, feed liquid mixing
Tank, acid storage tank, liquid alkaline storage tank, blower, heater, temperature sensor, dissolved oxygen meter, pH meter, transmissometer, liquidometer, TDS are supervised online
Survey instrument and processor；
It is equipped with fluorescent lamp in each enrichment culture case, is communicated with exhaust in the side wall upper part of each enrichment culture case
Pipe, is communicated with evacuated tube in the lower sidewall of each enrichment culture case, and exhaust-valve is equipped in the evacuated tube；Each
Bottom in the enrichment culture case is equipped with aeration tube, and aeration valve and aeration head, the every exposure are equipped in the aeration tube
Tracheae is connect with the blower and pipeline；The heater is equipped in each enrichment culture case, the temperature passes
Sensor, the dissolved oxygen meter, the pH meter, the transmissometer, the liquidometer and the TDS on-line computing model；
The adjacent enrichment culture case is connected to by pipeline, is equipped on the pipeline for being connected to the adjacent enrichment culture case and is turned liquid
Valve；
The outlet of the feed liquid blending tank is connected to the entrance of feed pump, the outlet of the feed pump respectively with each enrichment
The import of incubator is connected to by pipeline, is equipped with inlet valve on the pipeline for being connected to the feed pump and the enrichment culture case；
The outlet of the acid storage tank is connected to the entrance of sour metering pump, and the outlet of the liquid alkaline storage tank and the entrance of alkali metering pump connect
Logical, the outlet of the acid metering pump and the alkali metering pump is connected to each enrichment culture case by pH regulation pipe respectively,
PH regulating valve is equipped in each pH regulation pipe；
The temperature sensor, the dissolved oxygen meter, the pH meter, the transmissometer, the liquidometer, TDS on-line monitoring
Instrument and the electromagnetic flowmeter are electrically connected with the input terminal of the processor, the output end of the processor respectively with it is described into
Material pump, the aeration valve, the exhaust-valve, described turns liquid valve, the daylight at the inlet valve, the blower, the heater
Lamp, the sour metering pump, the alkali metering pump and pH regulating valve electrical connection.
2. a kind of automatic enrichment culture device of resistance to bacterium with high salt according to claim 1, which is characterized in that the enrichment culture
Case is four.
3. a kind of automatic enrichment culture device of resistance to bacterium with high salt according to claim 2, which is characterized in that it further includes culture
Base batch can and NaCl solution storage tank, the culture medium raw material tank outlet and the NaCl solution storage tank outlet with it is described
The entrance of feed liquid blending tank is connected to by pipeline, is set on the pipeline for being connected to the culture medium raw material tank and the feed liquid blending tank
There is culture medium control valve, be equipped with NaCl control valve on the pipeline for being connected to the NaCl solution storage tank and the feed liquid blending tank,
The culture medium control valve and the NaCl control valve are electrically connected with the output end of the processor.
4. a kind of automatic enrichment culture device of resistance to bacterium with high salt according to claim 3, which is characterized in that in the culture medium
It is equipped with culture medium blender in head tank, NaCl blender, the culture medium blender are equipped in the NaCl solution storage tank
It is electrically connected with the output end of the processor with the NaCl blender.
5. the automatic enrichment culture device of -4 any resistance to bacterium with high salt of one kind according to claim 1, which is characterized in that it is also wrapped
Include hydraulic mixing pipe and the return valve on the hydraulic mixing pipe and the hydraulic mixing pump in the evacuated tube, institute
It states hydraulic mixing pump and the return valve is electrically connected with the output end of the processor；One end of the hydraulic mixing pipe is set to
It is described between the other end of the hydraulic mixing pipe and hydraulic mixing pump and the return valve in the enrichment culture case
Evacuated tube connection.
6. a kind of automatic enrichment culture device of resistance to bacterium with high salt according to claim 1, which is characterized in that it further includes electromagnetism
Flowmeter, the electromagnetic flowmeter are set on the pipeline for being connected to the blower and the aeration tube.
7. utilizing the resistance to bacterium with high salt of automatic enrichment culture device enrichment culture of any resistance to bacterium with high salt of one kind of claim 1-6
Method, which is characterized in that it is comprised the following steps:
Process one: culture medium is prepared
Culture medium is mixed in feed liquid mixing tank in advance with NaCl, obtains the culture medium solution of appropriate salt content, then successively
Each enrichment culture case is added, controls 15-40 DEG C of temperature of culture medium solution described in each enrichment culture case, pH 6.0-
9.0, dissolved oxygen 0.03-0.2mmol/L；The TDS of culture medium solution described in the adjacent enrichment culture case increases step by step, and phase
The TDS amplification of culture medium solution described in the adjacent enrichment culture case is not more than 20000mg/L；Wherein, it is enriched with described in the first order
The TDS of culture medium solution described in incubator is 5000mg/L~10000mg/L；
Process two: resistance to bacterium enrichment culture with high salt
It adds gram-positive bacteria in the enrichment culture case described in the first order to be cultivated, in enrichment culture case described in the first order
Bacteria concentration reach 1010When cFU/ml or more, strain is in growth stationary phase, takes or enter enrichment culture case described in the second level
Continue enrichment culture；It repeats the above steps, obtains the resistance to bacterium with high salt for handling different salt content waste water；
In the process two: in resistance to bacterium enrichment culture process with high salt, controlling culture medium solution described in each enrichment culture case
15-40 DEG C of temperature, pH 6.0-9.0, dissolved oxygen 0.03-0.2mmol/L.
8. a kind of automatic Enrichment culture method of resistance to bacterium with high salt according to claim 7, which is characterized in that pH meter real-time monitoring
The pH of the culture medium solution, feedback processor controls the opening and closing of sour metering pump or alkali metering pump, and then effectively adjusts the training
The pH of based sols is supported, specific:
When the pH of the culture medium solution reaches the setting value upper limit, the sour metering pump is opened, being pumped into HCl reduces pH；
When the pH of the culture medium solution reaches setting value lower limit, the alkali metering pump is opened, liquid alkaline is pumped into and improves pH.
9. a kind of automatic Enrichment culture method of resistance to bacterium with high salt according to claim 7, which is characterized in that dissolved oxygen meter is supervised in real time
The dissolved oxygen amount for surveying the culture medium solution feeds back the processor, controls the opening and closing of the start and stop of blower with corresponding aeration valve.
10. a kind of automatic Enrichment culture method of resistance to bacterium with high salt according to claim 7, which is characterized in that temperature sensor
The temperature of culture medium solution described in real-time monitoring feeds back the processor, controls the opening and closing of heater or hydraulic mixing pump, adjusts
The temperature of the culture medium solution.
11. a kind of automatic Enrichment culture method of resistance to bacterium with high salt according to claim 7, which is characterized in that in the process
Two: in resistance to bacterium enrichment culture with high salt, the TDS of culture medium solution described in TDS on-line computing model real-time monitoring feeds back the processing
Device, when the TDS content of the culture medium solution is higher than the setting value upper limit, Primary culture base control valve, feed pump and corresponding
Inlet valve, when the TDS content of the culture medium solution reaches setting value lower limit, close the culture medium control valve, it is described into
Material pump and the corresponding inlet valve.
12. a kind of automatic Enrichment culture method of resistance to bacterium with high salt according to claim 7, which is characterized in that control is each described
25-35 DEG C of the temperature of culture medium solution described in enrichment culture case.
13. a kind of automatic Enrichment culture method of resistance to bacterium with high salt according to claim 7, which is characterized in that control is each described
The dissolved oxygen 0.05-0.2mmol/L of culture medium solution described in enrichment culture case.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110564605A (en) * | 2019-09-17 | 2019-12-13 | 山东昱泰环保工程有限公司 | Semi-open type microbial fermentation equipment and method for high-salinity wastewater treatment |
| CN112828031A (en) * | 2021-01-05 | 2021-05-25 | 北京科技大学 | System for reinforcing cemented filling material by using bacteria and use method |
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| KR200472329Y1 (en) * | 2012-12-12 | 2014-04-16 | 이재학 | device for culturing microorganism |
| CN206359326U (en) * | 2016-11-18 | 2017-07-28 | 北京美斯顿科技开发有限公司 | It is a kind of to be used to handle the high Facultative Halophiles domestication of high-salt wastewater, device for supplying |
| CN207828309U (en) * | 2017-12-20 | 2018-09-07 | 内蒙古久科康瑞环保科技有限公司 | A kind of automatic enrichment culture device of resistance to bacterium with high salt |
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| JPH07203945A (en) * | 1994-01-12 | 1995-08-08 | Hitachi Ltd | Apparatus for culturing cell of living body |
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| KR200472329Y1 (en) * | 2012-12-12 | 2014-04-16 | 이재학 | device for culturing microorganism |
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| CN110564605A (en) * | 2019-09-17 | 2019-12-13 | 山东昱泰环保工程有限公司 | Semi-open type microbial fermentation equipment and method for high-salinity wastewater treatment |
| CN112828031A (en) * | 2021-01-05 | 2021-05-25 | 北京科技大学 | System for reinforcing cemented filling material by using bacteria and use method |
| CN112828031B (en) * | 2021-01-05 | 2022-02-22 | 北京科技大学 | System for reinforcing cemented filling material by using bacteria and use method |
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