KR200472329Y1

KR200472329Y1 - device for culturing microorganism

Info

Publication number: KR200472329Y1
Application number: KR2020120011732U
Authority: KR
Inventors: 노형권; 이재학
Original assignee: 이재학; 노형권
Priority date: 2012-12-12
Filing date: 2012-12-12
Publication date: 2014-04-16

Abstract

The present invention relates to a microbial culture apparatus, and integrally configured to include a water level detection unit, a temperature control unit, an acid unit, a water spray unit, and a float removal unit in the culture tank, to control the water level and temperature in the culture tank, The watering and flotation process makes it possible to cultivate and propagate microorganisms more stably and uniformly, while simplifying and minimizing the configuration of the device to reduce facility costs and site use, and to reduce maintenance costs. Has an effect.

Description

Device for culturing microorganism

The present invention relates to a microbial culture apparatus, and more particularly, to a microbial culture apparatus for culturing and propagating microorganisms more stably and uniformly.

As is well known, with the growing interest in environmentally friendly farming methods, efforts are being made to protect the environment from the use of pesticides indiscriminately and to provide consumers with healthy food materials grown with environmentally friendly farming methods.

Microorganisms are commonly used materials for environmentally friendly farming. The microbial agent is usually cultivated in large quantities through a microbial culture apparatus provided in a farmhouse and then periodically supplied to the growing area.

Microbial agents are usually used to prevent pests, but their use is constantly being expanded, and thus the demand thereof is gradually increasing.

However, because of the high technology and the complicated processing process, the conventional method and maintenance of the microorganism cultivation apparatus requires high facility and maintenance costs, as well as high cost and site for the installation of the apparatus, as well as temperature change and flow rate. There is a problem that the difference in processing efficiency caused by the change is severe.

In addition, in order to stabilize sludge generated in the culture tank, a separate fire extinguishing facility should be included, and due to the odor generation and secondary decomposition of sludge, the treatment plant was recognized as a hate facility or pollution generating facility, and nitrogen as a hardly decomposable substance. And it is difficult to remove the phosphorus has a problem for the water quality conservation of the basin stream.

The purpose of the present invention to solve the above problems, to make the culture and propagation of microorganisms more stable and uniform, to simplify and miniaturize the composition to reduce the cost of the facility and site use, and to reduce the maintenance cost It is to provide a microbial culture apparatus that allows.

Microbial culture apparatus of the present invention for achieving the above object, the tubular culture tank having an inner receiving space for microbial culture; A culture water supply unit supplying culture water for culturing microorganisms in the culture tank; A water level detection unit installed in the culture tank to detect a level of the culture water filled in the culture tank by the culture water supply unit; A temperature controller for sensing and adjusting the temperature of the culture water filled in the culture tank; An air dispersing unit installed under the culture tank to diffuse air bubbles in the culture water so as to uniformly mix and culture microorganisms; A sprinkling part installed at an upper side of the culture tank to circulate the culture solution during the microbial culture to spray bubbles to uniformly disperse the microorganisms; Microbial culture medium discharge unit for discharging the microbial culture medium cultured in the culture tank; And a float removal unit configured to wash the inside of the culture tank in which the microbial culture solution is discharged to remove residual suspended matter.

Here, the culture water supply unit is a first water supply pipe is hypothesized to supply to the culture water in the culture tank; A water supply pump installed on the first water supply pipe; And a first supply flow rate control valve installed between the water supply pump and the culture tank on the first water supply pipe.

In addition, the water level detector is a liquid column installed vertically along the outer wall of the culture tank; And a water level sensor installed in the culture tank.

The temperature controller may include a temperature sensor installed inside the culture tank; And

It may be configured to include; a heater is installed to penetrate the lower side of the culture tank to heat the culture water in the culture tank.

In addition, the air diffuser is an air diffuser pipe which is installed in the inner lower side of the culture tank; An diffuser connection pipe passing through the culture tank and connecting the diffuser pipe; And a blower fixed to one side of the culture tank and installed on the diffuser connection pipe, wherein the diffuser pipe is branched in at least two radial directions on a plane about an end of the diffuser connection pipe.

In addition, the sprinkling portion penetrates one side of the culture tank and the culture solution introduction pipe is installed to introduce the microbial culture solution in the culture tank; A culture solution inlet quantity control valve installed outside the culture tank on the culture solution inlet pipe; A watering pipe fixedly installed at an upper side of the culture tank; And a self-suction pump connecting the culture solution inlet pipe and the watering pipe to self-circulate the microbial culture solution in the culture tank to sprinkle the water, wherein the watering pipe has a ring shape corresponding to the cross-sectional shape of the culture tank. Edge ring sprinkling pipe portion consisting of; And a center straight sprinkling pipe part intersecting and connecting the inside of the edge ring sprinkling pipe part with each other.

In addition, the microbial culture medium discharge part is connected to the microbial culture medium discharge pipe passing through the lower side of the culture tank; And a microbial culture liquid discharge pump installed on the microbial culture liquid discharge pipe. And a culture solution discharge flow rate control valve installed between the culture tank and the microbial culture solution discharge pump on the microbial culture solution discharge pipe.

The float removal unit may be branched from the water supply pump of the first water supply pipe and the first water supply valve to connect the water spray pipe, and to wash the float inside the culture tank through the water spray pipe. A second water supply pipe configured to spray washing water for the washing machine; A second water supply control valve installed on the second water supply pipe; Washing water discharge pipe is installed through the lower side of the culture tank; And a washing water discharge amount control valve installed on the washing water discharge pipe.

According to the microorganism culturing apparatus of the present invention described above, it is integrally configured to include a water level detection unit, a temperature control unit, an acid unit, a watering unit, and a floating material removing unit in the culture tank so as to control the water level and temperature in the culture tank In order to cultivate and propagate microorganisms more stably and uniformly through the process of sprinkling and flotation, it is possible to reduce the installation cost and site use by simplifying and minimizing the configuration of the device and to reduce maintenance costs. It has an effect.

1 is a side cross-sectional view showing a microbial culture apparatus according to an embodiment of the present invention.
FIG. 2 is a plan sectional view showing an air conditioner pipe installation state of the microorganism culture apparatus cut along the line II-II of FIG. 1.
3 is a partial side cross-sectional view illustrating a process of dispersing air in the form of bubbles through the diffuser pipe of FIG. 2.
4 is a plan sectional view of the microorganism culture apparatus cut along the line IV-IV of FIG.
Figure 5 is a partial side cross-sectional view showing the microbial culture solution watering process of the watering unit.
Figure 6 is a schematic diagram showing the operating state of the suspended matter removal unit of the microbial culture apparatus.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art may easily implement the present invention. However, the present invention may be embodied in many different forms and is not limited to the embodiments described herein. In order to clearly illustrate the present invention in the drawings, parts not related to the description are omitted, and the same or similar components are denoted by the same reference numerals throughout the specification.

1 is a side cross-sectional view showing a microbial culture apparatus according to an embodiment of the present invention.

Referring to Figure 1, the microbial culture apparatus 1 of the present embodiment is a culture tank 10, the culture water supply unit 20, the water level detection unit 30, the temperature control unit 42, the diffuser unit 50 , Watering unit 60, microbial culture medium discharge unit 70, is configured to include a float removal unit (80).

The culture tank 10 is made of a closed stainless steel tubular shape having an inner receiving space for microbial culture, and the upper cover portion 11 injects microorganisms into the supplied culture water and performs internal cleaning and observation. A through hole 12 is formed.

On the other hand, the blower 51 of the diffuser 50 and the self-suction pump 62 of the sprinkling part 60 which are mentioned later are fixedly installed in the upper part of the cover part of the culture tank 10, respectively.

The culture water supply unit 20 is configured to include a first water supply pipe 21, a water supply pump 22, and a first water supply control valve 23, and the microorganisms in the culture tank 10 using ground water. Supply to culture water for culture.

The first water supply pipe 21 is hypothesized to be connected through the upper side of the side wall surface of the culture tank 10 to supply the culture water in the culture tank 10 by using the ground water.

The water supply pump 22 is installed on the first water supply pipe 21 to provide the water supply pressure to supply the groundwater to the culture tank 10 through the first water supply pipe 21.

The first water supply control valve 23 is installed between the water supply pump 22 and the culture tank 10 on the first water supply pipe 21 to provide a culture tank (the water supply pump 22). 10) Control the quantity of groundwater supplied into the system.

The water level detector 30 is composed of a liquid column meter 31 and a water level sensor 32, and detects the water level of the culture water supplied to the culture tank 10 through the culture water supply unit 20 to fill. Do it.

The liquid column meter 31 is vertically installed along the outer wall of the culture tank 10 so that the number of culture water filled in the culture tank 10 and the amount of the microbial culture solution in which the microorganisms are cultured can be visually observed from the outside.

And, the water level sensor 32 is installed to hang at a predetermined height inside the culture tank 10, and continuously measure and monitor the quantity of the culture water filled in the culture tank 10 and the microbial culture medium in which the microorganisms have been cultured thereafter. Do it.

Here, the water supply pump 22 and the first water supply control valve 23 of the culture water supply unit 20 is interlocked with the water level detection sensor 32 of the water level detection unit 30 and a control box (not shown). It may be automatically controlled by so that the culture water for culturing the microorganism to a predetermined level may be filled.

The temperature controller 40 is configured to include a temperature sensor 42 and a heating heater 42, so as to detect and adjust the temperature of the culture water and the microbial culture solution filled in the culture tank (10).

In addition, the temperature sensor 42 is installed to hang at a predetermined height inside the culture tank 10 to continuously measure the temperature of the culture water filled in the culture tank 10 and then the microbial culture medium cultured thereafter. Keep an eye on it.

The heating heater 41 is installed to penetrate through the central side of the side wall surface of the culture tank 10, and interlocked with the temperature sensor 42 and automatically controlled by a control box to fill the culture water 10 inside the culture tank 10. It can be maintained at a set temperature within the range of 25 ℃ to 35 ℃ suitable for the culture of microorganisms.

The air dispersing unit 50 is configured to include a blower 51, an air dispersing pipe 52, and an air dispersing pipe 53, to uniformly mix the microorganisms introduced into the culture water and to collect oxygen in the air of air for microbial culture. Supply air in the form of bubbles.

The diffuser pipe 52 is installed with a plurality of fine through holes in the lower side of the culture tank 10, and the diffuser connection pipe 53 vertically penetrates the cover part 11 of the culture tank 10 and blows the blower 51. And an extension installed to connect the diffuser pipe 52.

And, the blower 51 is fixed to the upper side of the cover of the culture tank 10 as described above, the air supplied through the diffuser connecting pipe 53 is the culture water through the fine through holes in the diffuser pipe 52 Air bubbles are diffused in the form of bubbles in the inside.

On the other hand, by installing the air supply flow rate control valve 54 at the end before the blower 51 connection of the diffuser connecting pipe 53, it is possible to adjust the amount of air diffused in the culture water.

Here, the blower 51 and the air supply flow rate control valve 54 may be automatically controlled by the control box and configured to be automatically operable at predetermined time units during the microbial culture period.

FIG. 2 is a cross-sectional plan view illustrating an air conditioner pipe installation state of the microbial culture apparatus cut along line II-II of FIG. 1, and FIG. 3 illustrates a process of air scattering in the form of air through the air diffuser pipe of FIG. 2. Partial side cross-sectional view.

Referring to FIGS. 2 and 3, the diffuser pipe 52 may be branched in at least two radial directions on a plane about the lower end of the diffuser connection pipe 53.

In the present embodiment, the diffuser pipe 52 illustrates a branched cross shape around the lower end of the diffuser connection pipe 53, but the present invention is not necessarily limited thereto and has a larger number of branches and a radial shape as necessary. Naturally, it can be formed by branching.

Therefore, the air supplied from the diffuser connecting pipe 53 through the diffuser pipe 52 is diffused in the form of fine bubbles in the lower part of the inside of the culture tank filled with the culture water, so that the microorganisms introduced into the culture water can be uniformly mixed in the culture water. In addition, the amount of oxygen required for culturing microorganisms can be sufficiently supplied into the culture tank.

Referring back to FIG. 1, the watering unit 60 is configured to include a culture solution inlet pipe 61, a culture solution inlet flow rate control valve 64, a watering pipe 63, and a self suction pump 62. During microbial culture, the microbial culture solution is self-circulated to sprinkle on the upper side of the culture tank 10 to remove bubbles generated during the microbial culture and to uniformly distribute the microorganisms in the culture tank.

The culture solution inlet pipe 61 vertically penetrates the lid of the culture tank 10 and is installed to connect to the self-circulation pump 62 for the self-circulation of the microbial culture in the culture tank 10.

The culture medium inlet quantity adjusting valve 64 adjusts the quantity of the microbial culture medium which is self-circulated by the self-circulating pump 62 installed outside the culture tank on the culture medium inlet pipe 61.

The sprinkling pipe 63 is fixedly installed by the support 13 at the upper side of the culture tank 10, and the microbial culture solution is installed at the upper side of the culture tank 10 through a plurality of micro nozzles (or micro holes). Spray to the bottom.

As described above, the self-suction pump 62 is fixed to the upper side of the cover of the culture tank 10 to connect the culture solution inlet pipe 61 and the watering pipe 62 and the microorganisms in the culture tank 10. The culture solution is self-circulated so that it can be sprayed into the culture tank 10.

4 is a plan sectional view of the microorganism culture apparatus cut along the line IV-IV of FIG. Figure 5 is a partial side cross-sectional view showing the microbial culture solution watering process of the watering unit.

4 and 5, the sprinkling pipe 63 has an edge ring-shaped sprinkling pipe portion 63a and a ring-shaped sprinkling pipe portion formed of a circular ring shape corresponding to the cross-sectional shape of the culture tank 10. It comprises a center straight sprinkling pipe portion (63b) that crosses the inside of the (63a) in a cross shape and connect with each other.

Therefore, by injecting the microbial culture liquid self-circulated through the culture solution inlet pipe 61 and the self-suction pump 62 of the spraying unit 60 from the inside of the culture tank 10 through the spraying pipe 53, the culture tank (10) It is possible to sink and remove bubbles generated when culturing microorganisms inside, and to uniformly disperse a large amount of microorganisms contained in the bubbles.

At this time, in a state in which the second feed water control valve 82 provided on the water supply pipe 81 of the float removing unit 80 to be described later is closed, the culture fluid inflow water control valve 64 of the culture fluid inlet pipe 61 is closed. ), And the autocirculation sprinkling process of the microbial culture solution by the self-suction pump 62 is performed, and the quantity of the microbial culture solution to be sprayed is controlled by the culture medium inlet quantity control valve 64.

Referring again to FIG. 1, the microbial culture medium discharge unit 70 is configured to include a microbial culture medium discharge pipe 71, a microbial culture medium discharge pump 72, and a culture medium discharge flow rate control valve 73. It is possible to discharge the microbial culture medium cultured in (10) to the outside of the culture tank (10).

The microbial culture solution discharge pipe 71 penetrates the lower side of the culture tank 10 and is installed to extend outside the culture tank 10 to discharge the microbial culture solution cultured in the culture tank 10.

At this time, the discharge end 71a of the microbial culture liquid discharge pipe 71 is bent to a predetermined height from the ground, so that it can be easily discharged and transported in a tank lorry mounted on a vehicle or the like.

The microbial culture medium discharge pump 72 is installed on the microbial culture medium discharge pipe 71 to provide a discharge pressure for discharging the microbial culture medium from the culture tank 10.

The culture medium discharge flow rate control valve 73 is installed between the culture tank 10 and the microbial culture medium discharge pump 72 on the microbial culture medium discharge pipe 71 to discharge the microorganisms discharged through the microbial culture medium discharge pipe 71. Control the discharge flow rate of the culture.

The microbial culture liquid discharge pump 72 and the culture liquid discharge flow rate control valve 73 are automatically controlled by a control box, so that the microbial culture liquid at a predetermined flow rate can be discharged from the culture tank 10.

Referring again to FIG. 1, the float removing unit 80 includes a second water supply pipe 81, a second supply water control valve 82, a wash water discharge pipe 83, and a washing water discharge water control valve 84. It is configured to include, to wash the inside of the culture tank 10 in which the microbial culture solution is discharged to remove residual suspended matters generated in the culture tank during the microbial culture process.

The second water supply pipe 81 is branched between the water supply pump 22 and the first water supply control valve 23 of the first water supply pipe 21 so as to connect the watering pipe 63. It is installed to spray the washing water (that is, groundwater) to wash off the suspended matter from the upper side of the culture tank 10 through the watering pipe 63.

A second water supply control valve 82 is installed on the second water supply pipe 81 to supply groundwater that is supplied to wash suspended matters inside the culture tank 10 through the second water supply pipe 81. Adjust the supply quantity.

Washing water discharge pipe (83) is installed through the lower side of the culture tank (10) is sprayed through the spraying pipe (63) to wash the inside of the culture tank (10) and the wash water collected in the lower portion to the outside of the culture tank (10) Allow for discharge.

The washing water discharge quantity control valve 84 is installed on the washing water discharge pipe 83 so that the washing water collected in the culture tank 10 may be discharged to the outside of the culture tank 10 through the washing water discharge pipe 83. To crack down.

Of course, the second feed water control valve 82 and the washing water discharge water control valve 84 is automatically controlled by the control box, and is controlled to operate in a predetermined time unit after the microbial culture medium discharge.

Figure 6 is a schematic diagram showing the operating state of the suspended matter removal unit of the microbial culture apparatus.

Referring to FIG. 6, the suspended matter removing unit 80 opens the second supply water control valve 23 in a state in which the first water supply control valve 23 is closed after the microbial culture solution is discharged to open the second water supply pipe. When the groundwater is supplied to the diffuser pipe through the washing machine, the suspended matter inside the culture tank is washed by the high pressure sprayed washing water into the culture tank through the diffuser pipe.

In addition, the washing water discharged through the washing water discharge valve 83 is discharged through the washing water discharge pipe 83 to collect the washing water collected together with the floats in the lower side of the culture tank 10.

Therefore, by using the microorganism culturing apparatus of the present embodiment, the incubation period of the microorganisms can be made faster and the configuration of the apparatus can be shortened and downsized, so that there is no need for other time waste and large site and building for microbial cultivation. The use will have the effect of improving the culture efficiency.

Although the preferred embodiments of the present invention have been described above, it is to be understood that the present invention is not limited thereto, but can be modified or changed in various ways within the scope of the claims for utility model registration, And it is of course also within the scope of this invention.

1: Microorganism Culture Device 10: Culture Tank
11: cover part 12: through hole
13: support 20: culture water supply unit
21: first water supply pipe 22: water supply pump
23: first supply flow rate control valve 30: water level detection unit
31: liquid meter 32: water level sensor
40: temperature controller 41: temperature sensor
42: heating heater 50: diffuser
51: blower 52: diffuser piping
53: diffuser connection piping 60: water spray
61: culture solution inlet pipe 62: self-suction pump
63: watering pipe 63a: edge ring-type watering pipe
63b: center straight sprinkling pipe 64: culture medium inlet flow control valve
70: microbial culture medium discharge portion 71: microbial culture medium discharge pipe
72: microbial culture medium discharge pump 73: culture medium discharge flow rate control valve
80: float removal unit 81: second water supply pipe
82: second supply water control valve 83: wash water discharge piping
84: flushing water discharge valve

Claims

A tubular culture tank having an inner receiving space for culturing microorganisms;
A culture water supply unit supplying culture water for culturing microorganisms in the culture tank;
A water level sensing unit installed in the culture tank to sense a level of the culture water filled in the culture tank by the culture water supply unit;
A temperature controller for sensing and adjusting the temperature of the culture water filled in the culture tank;
An air dispersing unit installed under the culture tank to diffuse air bubbles in the culture water so as to uniformly mix and culture microorganisms;
A sprinkling part installed at an upper side of the culture tank to circulate the culture solution during the microbial culture to spray bubbles to uniformly disperse bubbles and microorganisms;
Microbial culture medium discharge unit for discharging the microbial culture medium cultured in the culture tank; And
And a floating material removing unit for washing the inside of the culture tank in which the microbial culture solution is discharged to remove residual floating materials.

The sprayer
A culture solution inlet pipe passing through one side of the culture tank and installed to introduce the microbial culture solution in the culture tank;
A culture solution inlet quantity control valve installed outside the culture tank on the culture solution inlet pipe;
A watering pipe fixedly installed at an upper side of the culture tank; And
And a self-suction pump for connecting the culture solution inlet pipe and the watering pipe and allowing the microbial culture solution to circulate in the culture tank by self-circulation.

The watering pipe,
An edge ring sprinkling pipe portion having a ring shape corresponding to the cross-sectional shape of the culture tank; And
Microbial culture apparatus consisting of; central straight sprinkling pipe portion for connecting the inside of the edge ring-shaped sprinkling piping cross each other.

In claim 1,
The culture water supply unit,
A first water supply pipe which is hypothesized to be supplied to the culture water in the culture tank;
A water supply pump installed on the first water supply pipe; And
And a first supply water quantity control valve installed between the water supply pump and the culture tank on the first water supply pipe.

In claim 1,
The water level detection unit,
A liquid column system vertically installed along the outer wall of the culture tank; And
Microbial culture apparatus comprising; water level sensor installed in the culture tank.

In claim 1,
The temperature controller may include:
A temperature sensor installed inside the culture tank; And
And a heating heater passing through one side of the culture tank and heating the culture water in the culture tank.

In claim 1,
The acid-
An air diffuser pipe installed in the lower side of the culture tank;
An diffuser connection pipe passing through the culture tank and connecting the diffuser pipe; And
And a blower fixed to one side of the culture tank and installed on the diffuser connection pipe.

The diffuser pipe,
Microbial culture apparatus is formed branched in at least two radial directions on a plane around the end of the diffuser connecting pipe.

delete

In claim 1,
The microbial culture liquid discharge unit,
A microbial culture solution discharge pipe that is connected to and installed through one lower side of the culture tank; And
A microbial culture medium discharge pump installed on the microbial culture medium discharge pipe; And
And a culture medium discharge flow rate control valve installed between the culture tank and the microbial culture solution discharge pump on the microbial culture solution discharge pipe.

3. The method of claim 2,
The float removal unit,
Branched between the water supply pump and the first water supply valve of the first water supply pipe to connect the watering pipe, and to spray washing water for washing the suspended matter inside the culture tank through the watering pipe. A second water supply pipe;
A second water supply control valve installed on the second water supply pipe;
Washing water discharge pipe is installed through the lower side of the culture tank; And
Microbial culture apparatus comprising; washing water discharge amount control valve installed on the washing water discharge pipe.