CN101570736B - Bacteria capable of eliminating ammonian in micro-polluted source water under microthermal and aerobic conditions - Google Patents
Bacteria capable of eliminating ammonian in micro-polluted source water under microthermal and aerobic conditions Download PDFInfo
- Publication number
- CN101570736B CN101570736B CN2009100715696A CN200910071569A CN101570736B CN 101570736 B CN101570736 B CN 101570736B CN 2009100715696 A CN2009100715696 A CN 2009100715696A CN 200910071569 A CN200910071569 A CN 200910071569A CN 101570736 B CN101570736 B CN 101570736B
- Authority
- CN
- China
- Prior art keywords
- micro
- bacterium
- source water
- polluted source
- condition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention relates to bacteria capable of eliminating ammonian in micro-polluted source water under microthermal and aerobic condition and a screening and domestication method, relating to bacteriaand screening and domestication method and solving the problem that the existing bacteria are not suitable for the treatment of the micro-polluted source water and low temperature treatment. The bacterium SRA 13 of the invention is Brevibacterium mcbrellneri, belongs to brevibacterium and is collected in the CGMCC, the collection number is CGMCC No. 2890, and the collection date is 19th, January2009. The screening method includes the steps as follows: first, separation and purification; second, bacteria solution culture; third, the screening and the culture of the bacteria solution of survival strains; fifth, the progressive low-temperature domestication of the survival strains after the repetition of the step fourth; and sixth, the acquisition of bacteria solution of the survival strains after the low-temperature domestication, and secondary screening. The bacteria of the invention can treat the micro-polluted source water under the condition of low temperature of 2 to 10 DEG C.
Description
Technical field
The present invention relates to bacterium.
Background technology
Ammonia nitrogen (NH
3-N) with ionic state ammonia (NH
4 +) and non-ion state ammon (NH
3) two kinds of forms are present in the water.Chlorated ammonia can cause the problem of smelling flavor above 0.2mg/L in the tap water, also can reduce sterilisation effect, and ammonia through the nitrococcus effect, generates nitrite in water pipe, HUMAN HEALTH is brought harm.
Along with the research to the heterotrophic nitrification-aerobic denitrification aspect is progressively goed deep into, and isolate a large amount of heterotroph nitrifiers, as Peseudomonas spp, Alcaligenes faecalis, Thiosphaera pantotropha, Thauera mechemichensis etc.But in the above bacterial strain treating processes nitrite and Nitrate Accumulation phenomenon in the water outlet can take place, human body be had harm, and can produce N
2O causes secondary pollution to environment.Name is called in the patent of " method of ammonia nitrogen in denitrogenation acinetobacter calcoaceticus and the degrading waste water thereof (China Patent No.: ZL200410019474.7; the applying date: on 06 04th, 2004; Granted publication day: on 07 26th, 2006) " and discloses strain denitrogenation acinetobacter calcoaceticus (Acinetobacter sp) YY-5, CGMCCNo.1154, the method of ammonia nitrogen in the degrading waste water, can under aerobic condition, the ammonia nitrogen in the waste water be direct oxidation into nitrogen, influent ammonium concentration was all higher when but this bacterial strain was used, influent ammonium concentration is also more than 50mg/L when minimum, be not suitable for and handle the micro-polluted source water of ammonia nitrogen concentration below 5mg/L, and when degradation of ammonia nitrogen, controlled temperature is 15～40 ℃, be not suitable for subzero treatment, as the Heilongjiang Province, average temperature of the whole year is the highest to have only 4.9 ℃, and there is the time more than 6 months every year in Song Hua River, therefore water temperature is in below 10 ℃, uses above-mentioned bacterial classification period in winter at northern area and is difficult to reach processing requirements." China Environmental Science ", 2003; 23 (6): though 644～647 ammonia nitrogen removal effect is preferably arranged when the biological ceramic particle reactor is removed in the source water ammonia nitrogen under research low temperature under cold condition, caused a large amount of accumulation of nitrite, HUMAN HEALTH is brought harm.
Summary of the invention
The objective of the invention is in removing water, to have nitrite and Nitrate Accumulation phenomenon in the water outlet during ammonia nitrogen, produce N in order to solve existing bacterium
2O causes secondary pollution to environment and is not suitable for the problem of handling micro-polluted source water and subzero treatment, and the bacterium that can remove ammonia nitrogen in the micro-polluted source water under low temperature, aerobic condition is provided.
The bacterium SRA13 that can remove ammonia nitrogen in the micro-polluted source water under low temperature, aerobic condition is Brevibacterium mcbrellneri (a Mai Keburuide tyrothricin), belong to brevibacterium sp (mcbrellneri), in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, deposit number is CGMCC No.2890, and preservation date is on January 19th, 2009; The bacterium that low temperature, aerobic condition are removed ammonia nitrogen in the micro-polluted source water down is the Gram-negative aerobic bacteria, is rod-short, and length is 0.4 μ m～0.9 μ m, and wide is 0.7 μ m～1.0 μ m, and in pairs normal or cluster exists no gemma and flagellum, the motion of twitching; On beef-protein medium, form yellow bacterium colony, the bacterium colony circle, the bacterium colony surface elevation is smooth.
Can remove the screening acclimation method of ammonia nitrogen bacterium in the micro-polluted source water under low temperature, aerobic condition realizes according to the following steps: one, micro-polluted source water is got pregnant solution behind enrichment culture 48h under the aerobic condition of 28～32 ℃ of temperature, 150～170r/min, coat behind the pregnant solution gradient dilution on the LB solid medium, separation and Culture 48h under 28～32 ℃ condition, the different single bacterium colony of picking feature is inoculated in purifying cultivation 24h on the LB solid medium respectively then; Two, with the inoculation behind the purifying in the LB liquid nutrient medium, after cultivating 24h under 28～32 ℃ the condition, bacterium liquid; Three, get 1ml bacterium liquid and be inoculated in the screening culture medium, under 28～32 ℃ condition, cultivate 48h; Four, the bacterium liquid 1ml that gets survival strains transfers in fresh screening culture medium, cultivates 48h under 28～32 ℃ condition; Five, getting survival strains behind the repeating step 4 1～2 times is inoculated in and carries out progressively domestication by low temperature in the screening culture medium, progressively domestication by low temperature is to cultivate 48h under 20 ℃ condition, taming the bacterium liquid 1ml that gets survival strains after 3 generations is inoculated in the screening culture medium cultivates 48h under 15 ℃ condition, taming the bacterium liquid 1ml that gets survival strains after 3 generations again is inoculated in the screening culture medium cultivates 48h under 8 ℃ condition, taming the bacterium liquid 1ml that gets survival strains after 3 generations again is inoculated in the screening culture medium cultivates 48h under 6 ℃ condition, tame the bacterium liquid 1ml that gets survival strains after 3 generations again and be inoculated in the screening culture medium and under 2 ℃ condition, cultivate 48h, tamed for 3 generations; Six, get the bacterium liquid of survival strains after 2 ℃ of following domestications by low temperature of 5ml, centrifugal 5min under the 12000r/min rotating speed, taking precipitate also adds the 1ml sterilized water and makes suspension, add the 50ml distilled water that contains the 5g gac then, fixedly will fix germy gac taking-up behind the 48h down at 2 ℃, to fix germy gac again is inoculated in the sterilized water that contains the 5mg/L ammonia nitrogen, at 2 ℃, 8h vibrates under the condition of 140r/min, get the bacterial strain that has the ammonia nitrogen degradation ability and do not produce nitrate and nitrite then, being can be at low temperature, aerobic condition is removed the bacterium of ammonia nitrogen in the micro-polluted source water down; Wherein in the step 3 the every 1000mL of screening culture medium by the Na of 1.9g
2HPO
4, 2.0g KH
2PO
4, 0.01g MgSO
47H
2The CaCl of O, 0.005g
22H
2The NH of O, 5.0g
4The water of Cl and surplus is formed, and the pH value is 6.0～8.0, and the water of described surplus is micro-polluted source water.
Low temperature among the present invention, aerobic condition are removed the bacterium of ammonia nitrogen in the micro-polluted source water down, the catalase positive, oxidase negative, methyl red test feminine gender, do not produce acetyl methyl carbinol, do not produce H
2S, not hydrolyzed starch, liquefy gelatin, produce indoles, growth pH value is not 6～8, growth temperature is 2～30 ℃.
The bacterium of removing ammonia nitrogen in the micro-polluted source water among the present invention under screening domestication gained low temperature, the aerobic condition can be at 2～10 ℃ treating micro polluted source water under low temperature condition, and the ammonia nitrogen degradation rate reaches more than 90%, and nitrate-free and nitrite accumulation, does not produce N
2O.
The bacterium SRA13 that can remove ammonia nitrogen in the micro-polluted source water among the present invention under low temperature, aerobic condition is Brevibacterium mcbrellneri (a Mai Keburuide tyrothricin), belong to brevibacterium sp (mcbrellneri), in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, deposit number is CGMCC No.2890, and preservation date is on January 19th, 2009.
Description of drawings
Fig. 1 is the atomic force microscope observation figure that low temperature in the embodiment six, aerobic condition are removed ammonia nitrogen bacterium in the micro-polluted source water down.
Embodiment
Embodiment one: present embodiment can be removed ammonia nitrogen in the micro-polluted source water under low temperature, aerobic condition bacterium SRA13 is Brevibacterium mcbrellneri, belong to brevibacterium sp (mcbrellneri), in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, deposit number is CGMCC No.2890, and preservation date is on January 19th, 2009; The bacterium that low temperature, aerobic condition are removed ammonia nitrogen in the micro-polluted source water down is the Gram-negative aerobic bacteria, is rod-short, and length is 0.4 μ m～0.9 μ m, and wide is 0.7 μ m～1.0 μ m, and in pairs normal or cluster exists no gemma and flagellum, the motion of twitching; On beef-protein medium, form yellow bacterium colony, the bacterium colony circle, the bacterium colony surface elevation is smooth.
The bacterium that can remove ammonia nitrogen in the micro-polluted source water in the present embodiment under low temperature, aerobic condition is according to " the outstanding Bacteria Identification handbook preliminary judgement of uncle is an acinetobacter, is accredited as Mai Keburuide tyrothricin Brevibacterium mcbrellneri through the SHERLOCK microflora then.
Embodiment two: what present embodiment and embodiment two were different is the bacterium that can remove ammonia nitrogen in the micro-polluted source water under low temperature, aerobic condition, the catalase positive, oxidase negative; The methyl red test feminine gender does not produce acetyl methyl carbinol; Do not produce H
2S, not hydrolyzed starch, not liquefy gelatin; Produce indoles; Growth pH value is 6～8, and growth temperature is 2～30 ℃.Other is identical with embodiment two.
Embodiment three: present embodiment can be removed the screening acclimation method of ammonia nitrogen bacterium in the micro-polluted source water and realize according to the following steps under low temperature, aerobic condition: one, micro-polluted source water is got pregnant solution behind enrichment culture 48h under the aerobic condition of 28～32 ℃ of temperature, 150～170r/min, coat behind the pregnant solution gradient dilution on the LB solid medium, separation and Culture 48h under 28～32 ℃ condition, the different single bacterium colony of picking feature is inoculated in purifying cultivation 24h on the LB solid medium respectively then; Two, with the inoculation behind the purifying in the LB liquid nutrient medium, after cultivating 24h under 28～32 ℃ the condition, bacterium liquid; Three, get 1ml bacterium liquid and be inoculated in the screening culture medium, under 28～32 ℃ condition, cultivate 48h; Four, the bacterium liquid 1ml that gets survival strains transfers in fresh screening culture medium, cultivates 48h under 28～32 ℃ condition; Five, getting survival strains behind the repeating step 4 1～2 times is inoculated in and carries out progressively domestication by low temperature in the screening culture medium, progressively domestication by low temperature is to cultivate 48h under 20 ℃ condition, taming the bacterium liquid 1ml that gets survival strains after 3 generations is inoculated in the screening culture medium cultivates 48h under 15 ℃ condition, taming the bacterium liquid 1ml that gets survival strains after 3 generations again is inoculated in the screening culture medium cultivates 48h under 8 ℃ condition, taming the bacterium liquid 1ml that gets survival strains after 3 generations again is inoculated in the screening culture medium cultivates 48h under 6 ℃ condition, tame the bacterium liquid 1ml that gets survival strains after 3 generations again and be inoculated in the screening culture medium and under 2 ℃ condition, cultivate 48h, tamed for 3 generations; Six, get the bacterium liquid of survival strains after 2 ℃ of following domestications by low temperature of 5ml, centrifugal 5min under the 12000r/min rotating speed, taking precipitate also adds the 1ml sterilized water and makes suspension, add the 50ml distilled water that contains the 5g gac then, fixedly will fix germy gac taking-up behind the 48h down at 2 ℃, to fix germy gac again is inoculated in the sterilized water that contains the 5mg/L ammonia nitrogen, at 2 ℃, 8h vibrates under the condition of 140r/min, get the bacterial strain that has the ammonia nitrogen degradation ability and do not produce nitrate and nitrite then, being can be at low temperature, aerobic condition is removed the bacterium of ammonia nitrogen in the micro-polluted source water down; Wherein in the step 3 the every 1000mL of screening culture medium by the Na of 1.9g
2HPO
4, 2.0g KH
2PO
4, 0.01g MgSO
47H
2The CaCl of O, 0.005g
22H
2The NH of O, 5.0g
4The water of Cl and surplus is formed, and the pH value is 6.0～8.0, and the water of described surplus is micro-polluted source water.
The LB solid medium uses behind sterilization 15～30min down at 121 ℃ in the present embodiment step 1.
The LB liquid nutrient medium uses behind sterilization 15～30min down at 121 ℃ in the present embodiment step 2.
Screening culture medium is used behind sterilization 15～30min down at 121 ℃ in the present embodiment step 3.
Survival strains in the present embodiment step 4, five and six is to make to occur muddy bacterial strain in the screening culture medium.
The 50ml distilled water that contains the 5g gac in the present embodiment step 6 uses behind sterilization 20～30min down at 121 ℃.
The bacterium that the screening domestication obtains in the present embodiment can be at 2～10 ℃ treating micro polluted source water under low temperature condition, and the ammonia nitrogen degradation rate reaches more than 90%, and nitrate-free and nitrite accumulation, does not produce N
2O.
Embodiment four: present embodiment and embodiment three are different is that the every 1000mL of LB solid medium is soaked the agar of powder, 20g, 10g NaCl formed with water surplus by the yeast of the Tryptones of 10g, 5g in the step 1, and the pH value is 6.0～8.0.Other step and parameter are identical with embodiment three.
Embodiment five: present embodiment and embodiment four are different is that the every 1000mL of LB liquid nutrient medium is soaked by the yeast of the Tryptones of 10g, 5g and powder, 10gNaCl forms with water surplus in the step 2, and the pH value is 6.0～8.0.Other step and parameter are identical with embodiment four.
Embodiment six: present embodiment can be removed the screening method of ammonia nitrogen bacterium in the micro-polluted source water and realize according to the following steps under low temperature, aerobic condition: one, 6 micro polluted source water samples are got pregnant solution respectively behind enrichment culture 48h under the aerobic condition of 30 ℃ of temperature, 160r/min, coat behind the pregnant solution gradient dilution on the LB solid medium, separation and Culture 48h under 30 ℃ condition, the different single bacterium colony of picking feature is inoculated in purifying cultivation 24h on the LB solid medium respectively then; Two, with the inoculation behind the purifying in the LB liquid nutrient medium, after cultivating 24h under 30 ℃ the condition, bacterium liquid; Three, get 1ml bacterium liquid and be inoculated in the screening culture medium, under 30 ℃ condition, cultivate 48h; Four, the bacterium liquid 1ml that gets survival strains transfers in fresh screening culture medium, cultivates 48h under 30 ℃ condition; Five, getting survival strains behind the repeating step 42 times is inoculated in and carries out progressively domestication by low temperature in the screening culture medium, progressively domestication by low temperature is to cultivate 48h under 20 ℃ condition, taming the bacterium liquid 1ml that gets survival strains after 3 generations is inoculated in the screening culture medium cultivates 48h under 15 ℃ condition, taming the bacterium liquid 1ml that gets survival strains after 3 generations again is inoculated in the screening culture medium cultivates 48h under 8 ℃ condition, taming the bacterium liquid 1ml that gets survival strains after 3 generations again is inoculated in the screening culture medium cultivates 48h under 6 ℃ condition, tame the bacterium liquid 1ml that gets survival strains after 3 generations again and be inoculated in the screening culture medium and under 2 ℃ condition, cultivate 48h, tamed for 3 generations; Six, get the bacterium liquid of survival strains after 2 ℃ of following domestications by low temperature of 5ml, centrifugal 5min under the 12000r/min rotating speed, taking precipitate also adds the 1ml sterilized water and makes suspension, add the 50ml distilled water that contains the 5g gac then, fixedly will fix germy gac taking-up behind the 48h down at 2 ℃, to fix germy gac again is inoculated in the sterilized water that contains the 5mg/L ammonia nitrogen, at 2 ℃, 8h vibrates under the condition of 140r/min, get the bacterial strain that has the ammonia nitrogen degradation ability and do not produce nitrate and nitrite then, being can be at low temperature, aerobic condition is removed in the micro-polluted source water down and the bacterium of ammonia nitrogen; Wherein in the step 3 the every 1000mL of screening culture medium by the Na of 1.9g
2HPO
4, 2.0g KH
2PO
4, 0.01g MgSO
47H
2The CaCl of O, 0.005g
22H
2The NH of O, 5.0g
4The water of Cl and surplus is formed, and the pH value is 6.0～8.0, and the water of described surplus is micro-polluted source water.
The micro polluted source water sample is that riverine (river) fetches water 6 places of taking a sample altogether respectively every 10 meters from the upstream to downstream Yu Jiang (river) center in the present embodiment step 1.
Screening obtains the low temperature in the embodiment one in the present embodiment, the bacterium SRA13 that aerobic condition is removed ammonia nitrogen in the micro-polluted source water down is Brevibacterium mcbrellneri, as shown in Figure 1, and atrichia; After testing, handle micro-polluted source water under 2 ℃ condition, the ammonia nitrogen degradation rate reaches 94%, and nitrate-free and nitrite accumulation, does not produce N
2O.
Claims (1)
1. can under low temperature, aerobic condition, remove the bacterium of ammonia nitrogen in the micro-polluted source water, it is characterized in that it is Mai Keburuide tyrothricin (Brevibacterium mcbrellneri) that low temperature, aerobic condition are removed the bacterium SRA13 of ammonia nitrogen in the micro-polluted source water down, belong to brevibacterium sp, in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, deposit number is CGMCC No.2890, and preservation date is on January 19th, 2009; This bacterium is the Gram-negative aerobic bacteria, is rod-short, and length is 0.4 μ m～0.9 μ m, and wide is 0.7 μ m～1.0 μ m, and in pairs normal or cluster exists no gemma and flagellum, the motion of twitching; On beef-protein medium, form yellow bacterium colony, the bacterium colony circle, the bacterium colony surface elevation is smooth.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100715696A CN101570736B (en) | 2009-03-18 | 2009-03-18 | Bacteria capable of eliminating ammonian in micro-polluted source water under microthermal and aerobic conditions |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100715696A CN101570736B (en) | 2009-03-18 | 2009-03-18 | Bacteria capable of eliminating ammonian in micro-polluted source water under microthermal and aerobic conditions |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101570736A CN101570736A (en) | 2009-11-04 |
| CN101570736B true CN101570736B (en) | 2011-03-23 |
Family
ID=41230222
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009100715696A Expired - Fee Related CN101570736B (en) | 2009-03-18 | 2009-03-18 | Bacteria capable of eliminating ammonian in micro-polluted source water under microthermal and aerobic conditions |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101570736B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103224903A (en) * | 2013-05-22 | 2013-07-31 | 哈尔滨工业大学 | Low-temperature heterotrophic nitrification bacterium |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103288223B (en) * | 2013-06-09 | 2014-04-09 | 北京工业大学 | Ammonia oxidizing bacterium enriching device and method suitable for purifying micro-polluted water and application of ammonia oxidizing bacterium enriching device and method |
| CN108611292B (en) * | 2015-02-17 | 2020-02-07 | 中国海洋大学 | Preparation method and application of cold-resistant ammonia oxidizing bacteria immobilized biochar ball |
| CN108396000A (en) * | 2018-02-05 | 2018-08-14 | 中铁西北科学研究院有限公司西南分院 | A kind of preparation method of low temperature resistant garbage disposal composite bacteria agent |
| CN108862569A (en) * | 2018-06-15 | 2018-11-23 | 南京理工大学 | The acclimation method of active nitrifying sludge under a kind of low temperature |
| CN109665672B (en) * | 2019-01-25 | 2021-11-05 | 黑龙江大学 | Device for removing total nitrogen in low-temperature underground water in enhanced mode and underground water treatment method |
| CN111302502B (en) * | 2020-02-24 | 2021-07-27 | 河海大学 | Emission reduction method for nitrous oxide in lakeside zone |
| WO2022104772A1 (en) * | 2020-11-23 | 2022-05-27 | 南京溧水高新创业投资管理有限公司 | Cyanobacteria composting composite microbial agent and preparation method therefor |
| CN112851027B (en) * | 2021-01-13 | 2022-07-01 | 浙江工业大学 | Microbial denitrification method for high-salinity sewage |
-
2009
- 2009-03-18 CN CN2009100715696A patent/CN101570736B/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103224903A (en) * | 2013-05-22 | 2013-07-31 | 哈尔滨工业大学 | Low-temperature heterotrophic nitrification bacterium |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101570736A (en) | 2009-11-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101570736B (en) | Bacteria capable of eliminating ammonian in micro-polluted source water under microthermal and aerobic conditions | |
| CN101503665B (en) | Bacteria capable of removing organic matter and ammonia nitrogen in micro-polluted water source water under low temperature and aerobic condition, and screening and taming method | |
| CN101705202B (en) | Pseudomonas stutzeri YZN-001 for removing ammoniacal nitrogen, nitrate nitrogen and nitrous nitrogen in water body and application | |
| CN103695351B (en) | Acinetobacter baumannii and application thereof | |
| CN106520624A (en) | Pseudomonas mendocina MKC-02 strain and application of pseudomonas mendocina MKC-02 strain to waste water denitrification | |
| CN105861359A (en) | Heterotrophic nitrification-aerobic denitrification high temperature resisting strain for producing floc, and application thereof | |
| CN103074277B (en) | Denitrifying bacterium and application thereof | |
| CN105733998B (en) | Efficient denitrification strain with heterotrophic nitrification and aerobic denitrification capabilities | |
| CN104911130A (en) | Halomonas sp. with denitrogenation capability and application thereof | |
| CN103789232A (en) | Pseudomonas stutzeri for efficiently processing nitrogen-containing sewage and application thereof | |
| CN111793573B (en) | Shewanella alga strain with functions of heterotrophic nitrate heterotrophic reduction and autotrophic nitrate heterotrophic reduction to ammonium, culture method and application thereof | |
| CN107058150A (en) | One plant of human pallid bacillus FX02 bacterial strain and its application in denitrogenation of waste water | |
| CN102827787A (en) | Denitrifying phosphorus removal bacteria bacillus cereus H-hrb01 and screening method and application | |
| CN102268386A (en) | Ammonia oxidizing bacteria, separation method thereof and application thereof | |
| CN108060101A (en) | Dietzia maris W02-3a and its application in denitrogenation | |
| CN104480045B (en) | One plant of otitis pseudomonas strains and its application | |
| CN111088200B (en) | Heterotrophic nitrification aerobic denitrifying bacterium and application thereof in pig-raising wastewater treatment | |
| CN102864098A (en) | Denitrification phosphorus removal bacterium H-hrb02 as well as screening method and application thereof | |
| CN105441359B (en) | One bacillus licheniformis and its application | |
| CN113583897B (en) | Bacillus aryabhattai FL05 and application thereof | |
| CN101519643B (en) | Method for rapidly separating anaerobic denitrifying bacteria and particular primer thereby | |
| CN111471612B (en) | Rhodococcus ruber HDRR2Y for purifying inorganic nitrogen and phosphorus in seawater pond culture tail water and application thereof | |
| CN110184217B (en) | Salt-tolerant denitrifying bacterium taking nitrite as nitrogen source and application thereof | |
| CN113699058A (en) | Salt-tolerant heat-resistant ammonia oxidizing bacteria and separation method and application thereof | |
| CN110468066A (en) | A kind of aerobic denitrifying bacteria and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110323 Termination date: 20120318 |