A kind of fast enriching propagation and purifying are cultivated the device of anaerobic ammonia oxidizing bacteria
Technical field
The utility model relates to Environmental Biotechnology, specifically is the device that a kind of fast enriching propagation and purifying are cultivated anaerobic ammonia oxidizing bacteria.
Background technology
The nineteen ninety-five people have just found the existence of anaerobic ammonium oxidizing bacteria first.Different fully with nitrated-denitrification biology that people before are familiar with, this newfound ancient type bacterium is a kind of chemosynthetic autotroph bacterium, finish denitrogenation with a kind of novel pathways metabolism, under anaerobic namely, be electron donor with the ammonia nitrogen, nitrite nitrogen is as electron acceptor(EA), the final nitrogen that generates, the chemical energy that discharges in this process, is used for carrying out the fixing of inorganic carbon, and finishes other vital movement processes as the energy by this bacterium.Under the condition of using the Anammox denitrification process, only ammonia nitrogen only about half of in the waste water need be oxidized to nitrite nitrogen, react with half ammonia nitrogen of residue then and generate nitrogen and escape into atmosphere, thereby compared to traditional nitrated-denitrification biological denitrogenation technology, the Anammox denitrification process can reduce the supply of oxygen in a large number can finish biological denitrification process.In addition, because anaerobic ammonium oxidizing bacteria belongs to chemoautotrophy type bacterium, and ammonia nitrogen is by the nitrite nitrogen oxidation, the energy density of the reaction of common formation nitrogen is very low, the bacterial growth division is extremely slow, thereby make the Anammox denitrogenation not only not need additionally to add organic carbon source, and surplus sludge volume significantly reduces compared to traditional technology, even to keep sufficient sludge quantity under many circumstances be a normal work to do keeping the Anammox reaction, has to adopt special measure and technology to accelerate the fast breeding of anaerobic ammonium oxidizing bacteria for obtaining excess sludge sometimes.
The technology that the Anammox biological denitrification process is low as a kind of working cost, efficient is high more and more is subjected to the attention of countries in the world, and has carried out big quantity research.Finally move success though spent about 3 years based on first actual engineering of anaerobic ammonia oxidation process, also there is actual engineering to put into operation in succession on Germany, Japan, Australia and other places afterwards, but initial startup mud derive from Holland mostly first move the Rotterdam sewage work of this technology success, but the many practical problemss in this process application are still perplexing the research and development personnel of countries in the world.Enrichment anaerobic ammonium oxidizing bacteria and carry out fast breeding and purifying from the provenance that nature very disperses wherein, quantity is big, the high reactor seed sludge of purity is first difficult problem that people at first run into for production practice provide.
The microorganism of the aerobic and anaerobic ferment process before anaerobic ammonia oxidation microbiological is different from, itself will spend the plenty of time enrichment of microorganism, and this bacterial multiplication speed is very slow, and the startup of reactor often needs to expend the plenty of time.Particularly running into when this bacterium produced the material of toxic action, biological reactions rates reduces rapidly, even stop, in this case, if can not in time find, will cause a large amount of accumulation of nitrite nitrogen, thereby bacterium is produced toxic action, finally cause the failure of reactor operation, even the fresh mud of the renewed vaccination of having to restarts.So fast enriching, and can breed in a large number and purifying anaerobic ammoxidation activity mud, be that this process application and research need the at first problem of solution.
People reach the purpose that research and production application anaerobic ammonium oxidizing bacteria remove in the water too much nitrogen; at first be will be from the anaerobic ammoxidation activity mud of occurring in nature enrichment, purifying, propagation sufficient amount; so that its can be fast a large amount of unnecessary nitrogens of high nitrogenous water that go out are avoided the threat that body eutrophication pollutes with the protection receiving water body.But the method that many researchs all adopt the aerobic sludge sewage of direct usefulness, anaerobism sewage disposal granule sludge or sediment of pond to start reactor starts experimental Anammox denitrification reactor, though but also can carry out the Anammox denitrification process in the reactor this moment, but the content of anaerobic ammonium oxidizing bacteria is all lower in the reactor mud, and total amount of sludge is also fewer, thereby can not satisfy large-scale reactor to the needs of anaerobic oxidation denitrification activity mud, can't be applied to produce.
Chinese patent application book CN102145940A discloses " a kind of device and method of fast breeding anaerobic ammonia oxidizing bacteria " (1), CN102061281A discloses " utilizing the method for membrane bioreactor enrichment culture anaerobic ammonium oxidizing bacteria " (2), CN101735947A discloses " a kind of soil anaerobic ammonoxidation bacterium concentrating system of simulating natural environment " (3), these methods or be conceived to the fast breeding of anaerobic ammonia oxidizing bacteria, i.e. its inoculation be a small amount of anaerobic ammonium oxidizing bacteria (1) that enriching and purifying is good; Be with membrane bioreactor enrichment culture anaerobic ammonium oxidizing bacteria, the anaerobic ammonium oxidizing bacteria of dispersion is enriched on the film, improved bioaccumulation efficiency significantly, structure for membrane bioreactor there is no particular requirement, and the quantity of the anaerobic ammonium oxidizing bacteria that obtains and purity have much do not guarantee (2); Or with earth pillar enrichment anaerobic ammonium oxidizing bacteria anaerobic ammonium oxidizing bacteria is enriched in the earth pillar, be difficult to problem that the bacterium of enrichment is separated but after enrichment is finished, exist, can not obtain directly, easily all that quantity is big, the higher anaerobic ammoxidation activity mud of purity, thereby have certain restriction in actual applications.
The utility model content
The purpose of this utility model is to provide a kind of fast enriching propagation and purifying to cultivate the device of anaerobic ammonia oxidizing bacteria.This device can be realized holding back adhering to of anaerobic ammonium oxidizing bacteria, enrichment and propagation.
The technical scheme that realizes the utility model purpose is:
The device of a kind of fast enriching propagation and purifying cultivation anaerobic ammonia oxidizing bacteria preheats system, slot type biological reaction tank and filtering basin three parts by water inlet and is linked in sequence and forms.
Described water inlet preheats system and is made up of preheating pond body, water inlet pipe, heating arrangements, temperature measuring mechanism, temperature control device and peristaltic pump, heating arrangements and temperature measuring mechanism are arranged in the body of preheating pond, and peristaltic pump is communicated with pipeline with helical heating pipe and the slot type biological reaction tank of heating arrangements respectively.
Described slot type biological reaction tank is arranged on the cloth shape hanging type fibrous type filler more than two groups in the body of pond by Chi Tihe and the foam type wrapper plate that is located on the dignity of pond is formed, and is provided with baffle plate between cloth shape hanging type fibrous type filler.
Described filtering basin are arranged on the cloth shape hanging type fibrous type filler more than two groups in the body of pond by Chi Tiji and the foam type wrapper plate that is located on the dignity of pond is formed, and are provided with baffle plate between cloth shape hanging type fibrous type filler, and filtering basin are provided with water outlet.
Described slot type biological reaction tank is that the degree of depth is the rectangular parallelepiped Da Chi of 2-2.5m, and volume is 10-20m
3
The method that a kind of fast enriching propagation and purifying are cultivated anaerobic ammonia oxidizing bacteria comprises the steps:
(1) arranges one and preheat system, slot type biological reaction tank and the filtering basin three parts device that the fast enriching propagation formed and purifying cultivate anaerobic ammonia oxidizing bacteria that is linked in sequence by water inlet;
(2) in the slot type biological reaction tank, place a certain amount of aerobic activated sludge that filters through elutriation in advance, and it is mixed with nutritive medium, make anaerobic ammonia oxidizing bacteria propagation;
(3) in the slot type biological reaction tank, progressively feed the nutritive medium that preheats system's heating through water inlet;
(4) by being arranged on the cloth shape hanging type fibrous type filler in slot type biological reaction tank and the filtering basin, hold back anaerobic ammonia oxidizing bacteria, make its enrichment;
(5) after cultivating in 60-90 days, cover with henna anaerobic ammonia oxidizing bacteria at cloth shape hanging type fibrous type filler;
(6) cloth shape hanging type fibrous type filler is taken out, with useless nutrient solution wash-out, natural sedimentation, can obtain the Anammox denitrogenation mud of larger amt and higher degree.
Described nutritive medium be ammonia and nitrite in the weight part ratio of 1:1-1.3 and microorganism growth need micro-formulated, total nitrogen content is 100-600mg/L.
After testing, the SS of Anammox denitrogenation mud is about 1000-6000mg/L, 50-500L, purity 〉=80%.
The utility model has the advantages that:
(1) technical measures taked of the utility model comprise volume and the degree of depth that increases breeder reactor, can hold the enrichment of anaerobic ammoxidation activity mud and the proliferate of larger amt;
(2) adopt the auxiliary traverse baffle that forms of the strip multiporous fiber cloth filler that dangles, for the apposition growth of anaerobic ammonium oxidizing bacteria provides carrier, thereby the Anammox long to generation time plays the enriching and purifying effect of holding back, and is easy to will adhere to the separation of anaerobic ammonium oxidizing bacteria wash-out and collect after cultivating end;
(3) by baffling repeatedly, the nutrient solution water inlet that enters can fully be contacted, for the growth of anaerobic ammonium oxidizing bacteria provides the nutriment of abundance to guarantee to reach the purpose of fast breeding and purifying with thalline;
(4) darker pond body and adopt internal recycle to stir and mode that the water surface covers plastic foamboard has reduced dissolving in of oxygen in the air has guaranteed the anaerobic state in the pond, but the also abundant mixing of assurance muddy water;
(5) water inlet of the nutritive medium of artificial preparation is played the effect that screening and enrichment are bred to anaerobic ammonium oxidizing bacteria, the effect that then can play wash-out, screen out other assorted bacterium; Adopt the mode of water inlet pipe coiling and water-bath indirect heating both to keep the temperature in the reaction tank and can prevent the influence that direct heating carries out bacterial growth, nutritive medium, the mode that adopts water outlet to hold back has again further increased holding back of anaerobic ammonium oxidizing bacteria and taking full advantage of nutritive medium, thereby these apparatus and method can realize the target of fast enriching propagation and purifying anaerobic oxidation bacterium, thereby provide sufficient seed sludge for starting the Anammox denitrification reactor, realize starting fast the purpose that the oxidation oxidation reactor removes ammonia nitrogen and nitrite nitrogen in the waste water.
Description of drawings
Fig. 1 cultivates the structural representation of the device of anaerobic ammonia oxidizing bacteria for the utility model fast enriching propagation and purifying.
Among the figure: 1. spillway 11. water outlets 12. pipelines of water inlet pipe 2. heating arrangements 3. temperature control devices 4. temperature measuring mechanism 5. peristaltic pumps 6. foam type wrapper plates 7. baffle plates 8. cloth shape hanging type fibrous type fillers 9. water outlet overflow ports 10. filtering basin.
Embodiment
Embodiment:
With reference to Fig. 1, the device of a kind of fast enriching propagation and purifying cultivation anaerobic ammonia oxidizing bacteria preheats system, slot type biological reaction tank and filtering basin three parts by water inlet and is linked in sequence and forms.
Water inlet preheats system and is made up of preheating pond body, water inlet pipe 1, heating arrangements 2, temperature measuring mechanism 4, temperature control device 3 and peristaltic pump 5, heating arrangements 2 and temperature measuring mechanism 4 are arranged in the body of preheating pond, and peristaltic pump 5 is communicated with pipeline 12 with helical heating pipe and the slot type biological reaction tank of heating arrangements 2 respectively.
The slot type biological reaction tank is arranged on the cloth shape hanging type fibrous type filler 8 more than two groups in the body of pond by Chi Tihe and the foam type wrapper plate 6 that is located on the dignity of pond is formed, between cloth shape hanging type fibrous type filler 8, be provided with baffle plate 7, be provided with water outlet overflow port 9 at the slot type biological reaction tank.
Filtering basin are arranged on the cloth shape hanging type fibrous type filler 8 more than two groups in the body of pond by Chi Tiji and the foam type wrapper plate 6 that is located on the dignity of pond is formed, be provided with baffle plate 7 between cloth shape hanging type fibrous type filler 8, filtering basin are provided with spillway 10 and water outlet 11.
The slot type biological reaction tank is that the degree of depth is the rectangular parallelepiped Da Chi of 2-2.5m, and volume is 10-20m
3
It is 10 m that the nutrient solution that heats is pumped into volume
3Reaction tank in, place the cloth shape multiporous fiber stuffing frame of easy-offtaking, the excess sludge of the aerobic sewage work of adding 50L is as initial source and the startup internal recycle agitator of anaerobic ammonium oxidizing bacteria, connect the nutrient solution water inlet system, the nutrient solution for preparing is squeezed into reaction tank by 2L/ minute flow velocity with pump, every day 2 times, started the internal recycle agitator in one time 30 minutes, cultivated 60-90 days, when treating to cover with on the fibrous packing dirt settling russet, fibrous packing is taken out, the wash-out dirt settling, quiescent setting, skim supernatant liquor, stay the sorrel slurry of bottom, be the Anammox denitrification activity mud of high purity (content of anaerobic ammonia oxidizing bacteria 〉=80%).Once the influence of the obtainable Anammox amount of sludge cloth shape multiporous fiber filler quantity of being laid generally is that SS is the about 100L of bacterium liquid of 2000mg/L.