CN104016478B - A kind of method of quick startup anaerobic ammonia oxidation reactor - Google Patents
A kind of method of quick startup anaerobic ammonia oxidation reactor Download PDFInfo
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Abstract
A kind of method that the present invention relates to quick startup anaerobic ammonia oxidation reactor, belongs to Anammox reaction field.A kind of method of quick startup anaerobic ammonia oxidation reactor, using activated sludge as kind of a mud, with manual simulation nitrogenous effluent for culture fluid, running reactor at least 50 days continuously, wherein, described manual simulation nitrogenous effluent is made up of following component and each concentration of component is: ammonia nitrogen 30～60mg/L, nitrite nitrogen 30～60mg/L, potassium bicarbonate 125mg/L, potassium dihydrogen phosphate 54mg/L, ferrous sulfate 0.5～5mg/L.Operation is simple for the method, it is not necessary to any extra energy expenditure, ferrum element abundance used, be a kind of practicality, efficiently, the microorganism beneficiation technologies of environmental protection.
Description
Technical field
A kind of method that the present invention relates to quick startup anaerobic ammonia oxidation reactor, belongs to Anammox reaction field.
Background technology
The quickly efficiently concentrating started with anaerobic ammonia oxidizing bacteria of anaerobic ammonia oxidation reactor is emphasis and the focus of Anammox research field all the time.Owing to the doubling time of anaerobic ammonia oxidizing bacteria is long, sludge yield coefficient is low, limits its popularization and application.Domestic and international many scholars attempt to explore, by some diverse ways, the approach quickly starting and being enriched with anaerobic ammonia oxidizing bacteria, have accumulated many referential experiences, the focus of research is concentrated mainly on: choosing of anaerobic ammonia oxidation reactor seed sludge, is enriched with design and the improvement aspect of the reactor of anaerobic ammonia oxidizing bacteria.The research of these two aspects is all based on farthest improving holding of anaerobic ammonia oxidizing bacteria in reactor and stays this principle of rate, although achieve the quick startup of reactor, but it is slow fundamentally not solve anaerobic ammonia oxidizing bacteria growth rate, the problem that metabolic rate is low.
Summary of the invention
It is an object of the invention to the growth conditions by optimizing anaerobic ammonia oxidizing bacteria, promote the synthesis of relevant enzyme (diamine dehydrogenase) and improve its enzyme ' s reaction speeding, accelerate the growth rate of anaerobic ammonia oxidizing bacteria, thus providing a kind of economical for quickly starting anaerobic ammonia oxidation reactor, effective new technique.
A kind of method of quick startup anaerobic ammonia oxidation reactor, using activated sludge as kind of a mud, with manual simulation nitrogenous effluent for culture fluid, runs reactor at least 50 days continuously,
Wherein, described manual simulation nitrogenous effluent is made up of following component and each concentration of component is: ammonia nitrogen 30～60mg/L, nitrite nitrogen 30～60mg/L, potassium bicarbonate 125mg/L, potassium dihydrogen phosphate 54mg/L, ferrous sulfate 0.5～5mg/L.
In technique scheme, what described " anaerobic ammonia oxidation reactor " was known to those skilled in the art may be used for the reactor of Anammox reaction, such as fluidized-bed reactor, fixed bed reactors, biofilm reactor, sequencing batch reactor, gas stripping type reactor etc.；Described " activated sludge " is anaerobism or amphimicrobian activated sludge, it is preferred to denitrifying activated sludge.
In technique scheme, described ammonia nitrogen is preferably with (NH4)2SO4Add in manual simulation nitrogenous effluent for raw material；Described nitrite nitrogen is preferably with NaNO2Add in manual simulation nitrogenous effluent for raw material.
In technique scheme, it is preferable that the ratio of described kind of mud and culture fluid is 1g:10mL.
In technique scheme, it is preferable that run reactor 50～80 days continuously.
In technique scheme, it is preferable that in reactor, sludge concentration MLSS is 2.54g/L.
In the method for quick startup anaerobic ammonia oxidation reactor of the present invention, the concentration of preferred described ferrous sulfate is 5mg/L.
In the method for quick startup anaerobic ammonia oxidation reactor of the present invention, preferred ammonia nitrogen concentration is 50mg/L；Preferred nitrite nitrogen concentration is 50mg/L.
In the method for quick startup anaerobic ammonia oxidation reactor of the present invention, preferred reactor service condition is: inside reactor temperature is 35 ± 1 DEG C, and pH is 7.0～8.5, and dissolved oxygen is lower than 0.5mg/L, lucifuge.
The preferred reactor method of operation in the method for quick startup anaerobic ammonia oxidation reactor of the present invention: take continuous uninterrupted operation；On average within every three days, measure the concentration of ammonia nitrogen, nitrite nitrogen, nitrate nitrogen, pH value in Inlet and outlet water, and bacteria samples is carried out thick enzyme extraction, DNA analysis.
The concentration of ammonia nitrogen, nitrite nitrogen, nitrate nitrogen in results of regular determination Inlet and outlet water of the present invention, pH value, and bacteria samples is carried out thick enzyme extraction (metabolic activity with quantitatively characterizing anaerobic ammonia oxidizing bacteria), DNA analysis (to characterize structure of community and the dynamically change thereof of microorganism species in reactor).
The invention have the benefit that
1. ferrum element (ferrous iron) is in the growth and metabolic process of anaerobic ammonia oxidizing bacteria, serves extremely important effect.As constituent and the active center of many functional enzymes (such as diamine synzyme, diamine dehydrogenase etc.), almost take part in all of important metabolic response of anaerobic ammonia oxidizing bacteria.By controlling ferrous concentration in culture matrix, it is possible to be effectively improved the metabolic rate of anaerobic ammonia oxidizing bacteria, accelerate growth and the enrichment rate of thalline, substantially reduce the startup time of anaerobic ammonia oxidation reactor.When running the 26th day, in reactor, occur that Anammox reacts；After 50 days cultivate, reactor successfully starts up and can stable operation.And existing major part technology, it usually needs the startup that anaerobic ammonia oxidation reactor could be realized in 70～120 days.
2. being enriched with, by this technology, the anaerobic ammonium oxidizing bacteria that obtains, in its born of the same parents, content of hemachrome is greatly increased so that diamine dehydrogenase there is higher activity, therefore reactor has efficient, stable nitrogen removal performance.
3. this technical operation is simple, it is not necessary to any extra energy expenditure, ferrum element abundance used, be a kind of practicality, efficiently, the microorganism beneficiation technologies of environmental protection.
Accompanying drawing explanation
Fig. 1 is reactor service chart, and accompanying drawing labelling is as follows: 1, outlet pipe, 2, water bath heating device, 3, reactor, 4, peristaltic pump, 5, water inlet pipe, 6, nitrogen storage tank.
Reactor used 3 is upflow sludge bed reactor, adopts lucite post to make, and upper end is added a cover and connects outlet pipe 1, lower end is added a cover and connects peristaltic pump 4 and water inlet pipe 5, and internal diameter is 50mm, height 255mm, dischargeable capacity 0.5L.The outer wall of reactor 3 water-bath sleeve pipe wraps up, and described water-bath sleeve pipe is connected with external water bath heating device 2 by flexible pipe, makes inside reactor keep stationary temperature.For avoiding the illumination inhibitory action to anaerobic ammonium oxidizing bacteria, reactor 3 is outside with black plastic cloth parcel.Described water inlet pipe 5 communicates with water inlet storage tank, and the outlet connecting pipe road of nitrogen storage tank 6, the end of its pipeline submerges under the liquid level of the inner inlet of water storage tank.
Fig. 2 is variable concentrations Fe2+Under condition, the startup time comparison diagram needed for each reactor, wherein, a is embodiment 1 (Fe2+Concentration 0.03mM), b and c respectively embodiment 2 and embodiment 3 (ferrous iron concentration respectively 0.06,0.09mM)；
In Fig. 3 (a), (b), (c) the respectively starting period, each inside reactor nitrogen element (ammonia nitrogen, nitrite nitrogen and nitrate nitrogen) concentration is schemed over time；
Fig. 4 is the growth changing trend diagram of each inside reactor anaerobic ammonia oxidizing bacteria 16SrRNA gene copy number；Wherein, a is embodiment 1 (Fe2+Concentration 0.03mM), b and c respectively embodiment 2 and embodiment 3 (ferrous iron concentration respectively 0.06,0.09mM)；
Fig. 5 is the growth trend figure of each inside reactor anaerobic ammonia oxidizing bacteria haemachrome C (hemec) concentration；Wherein, a is embodiment 1 (Fe2+Concentration 0.03mM), b and c respectively embodiment 2 and embodiment 3 (ferrous iron concentration respectively 0.06,0.09mM)；
Fig. 6 is the activity change trendgram of each inside reactor anaerobic ammonia oxidizing bacteria diamine dehydrogenase (HDH).Wherein, a is embodiment 1 (Fe2+Concentration 0.03mM), b and c respectively embodiment 2 and embodiment 3 (ferrous iron concentration respectively 0.06,0.09mM)；
Detailed description of the invention
Following non-limiting example can make those of ordinary skill in the art more fully understand the present invention, but does not limit the present invention in any way.
Test method described in following embodiment, if no special instructions, is conventional method；Described reagent and material, if no special instructions, all commercially obtain.
Embodiment 1
A kind of method of quick startup anaerobic ammonia oxidation reactor, comprises the steps:
1. the choosing of seed sludge: choosing the activated sludge of Ling Shui sewage treatment plant of Daliang City as kind of a mud, in reactor, sludge concentration MLSS is 2.54g/L.
2. reactor: reactor is upflow type anaerobic ammonia oxidation reactor, it is made up of PVC (polrvinyl chloride) material, working volume 0.5 liter (internal diameter 5cm, height 25.5cm).
3. the constituent of culture fluid and concentration:
4. reactor service condition: controlling inside reactor temperature is 35 ± 1 DEG C, and pH scope is between 7.0～8.5, and dissolved oxygen is lower than 0.5mg/L, lucifuge.
5. the reactor method of operation: take continuous uninterrupted operation, in reactor, the ratio of mud and culture fluid is 1g:10mL.Within the starting period, initial setting hydraulic detention time (HRT) is 12 hours, and in culture fluid, the concentration of ammonia nitrogen and nitrite nitrogen is 50mg/L, Fe (II) concentration and is always 0.03mM.Along with the improving constantly of TN removel rate of reactor, run to the 60th day, shorten HRT to 9.26 hour.Run the 70th day, shorten HRT to 6.67 hour further.The concentration of ammonia nitrogen, nitrite nitrogen, nitrate nitrogen in results of regular determination Inlet and outlet water, pH value, and bacteria samples is carried out thick enzyme extraction, DNA analysis；Completing the startup of anaerobic ammonia oxidation reactor, concrete outcome is in Table 1.
Embodiment 2
Other are identical with embodiment 1, the difference is that the concentration of Fe (II) is 0.06mM.
Embodiment 3
Other are identical with embodiment 1, the difference is that the concentration of Fe (II) is 0.09mM.
Table 1
As it can be seen from table 1 adopt this technology can accelerate the enrichment rate of anaerobic ammonia oxidizing bacteria, shortening the startup cycle about 30% of anaerobic ammonia oxidation reactor, the object bacteria of embodiment 3 gained performance in every respect is superior to embodiment 1 and embodiment 2.This result shows, by regulating Fe (II) concentration in culture matrix, the metabolic rate of anaerobic ammonia oxidizing bacteria can be effectively facilitated, be conducive to the synthesis of hemec (heme c) in its cell, and then the activity of raising diamine synzyme (HDH), finally realize the fast enriching of anaerobic ammonia oxidizing bacteria.
Claims (5)
1. a method for quick startup anaerobic ammonia oxidation reactor, using activated sludge as kind of a mud, with manual simulation nitrogenous effluent for culture fluid, runs reactor at least 50 days continuously,
Wherein, described manual simulation nitrogenous effluent is made up of following component and each concentration of component is: ammonia nitrogen 30～60mg/L, nitrite nitrogen 30～60mg/L, potassium bicarbonate 125mg/L, potassium dihydrogen phosphate 54mg/L, FeSO4·7H2O-EDTA 2Na, counts with the mass concentration of Fe (II) for 3.36 or 5.04mg/L.
2. method according to claim 1, it is characterised in that: described ammonia nitrogen concentration is 50mg/L；Described nitrite nitrogen concentration is 50mg/L.
3. method according to claim 1, it is characterised in that: reactor service condition is: inside reactor temperature is 35 ± 1 DEG C, and pH is 7.0～8.5, and dissolved oxygen is lower than 0.5mg/L, lucifuge.
4. method according to claim 1, it is characterised in that: the reactor method of operation: take continuous uninterrupted operation；On average every three days water samplings, measure the concentration of ammonia nitrogen, nitrite nitrogen, nitrate nitrogen, pH value in Inlet and outlet water, and bacteria samples are carried out thick enzyme extraction, DNA analysis.
5. method according to claim 1, it is characterised in that: the ratio of described kind of mud and culture fluid is 1g:10mL.
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| CN104450558B (en) * | 2014-10-30 | 2017-07-18 | 大连海洋大学 | A kind of Enrichment culture method of ocean anaerobic ammonia oxidizing bacteria |
| CN105923795A (en) * | 2016-07-04 | 2016-09-07 | 合肥工业大学 | Method for rapidly culturing anaerobic ammonium oxidation bacteria |
| CN109420663A (en) * | 2017-08-29 | 2019-03-05 | 浙江科罗尼生物科技有限公司 | Ferrous application and processing organic waste composition |
| CN109420664A (en) * | 2017-08-29 | 2019-03-05 | 浙江科罗尼生物科技有限公司 | Application of the ferrous iron in terms of promoting organic waste processing microflora fermentation |
| CN111960538B (en) * | 2020-09-02 | 2024-03-12 | 北京城市排水集团有限责任公司 | System and method for realizing stable operation of low ammonia nitrogen wastewater short-cut nitrification-anaerobic ammonia oxidation denitrification |
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