CN115152670B - Domestication method of salt-tolerant mandarin fish fries - Google Patents
Domestication method of salt-tolerant mandarin fish fries Download PDFInfo
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- 238000005273 aeration Methods 0.000 claims description 4
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- 239000001301 oxygen Substances 0.000 claims description 4
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- 241000238553 Litopenaeus vannamei Species 0.000 description 3
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- 241000883303 Acipenser sinensis Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
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- 241000276707 Tilapia Species 0.000 description 2
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- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 241001327682 Oncorhynchus mykiss irideus Species 0.000 description 1
- 241000264848 Siniperca Species 0.000 description 1
- 241001439624 Trichina Species 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention provides a salt-tolerant mandarin fish fry domestication method, which comprises the following steps: culturing mandarin fish in a fresh water pond, and feeding live baits every day to adapt mandarin fish to the environment; mixing with high salinity water and fresh water; feeding mandarin fish seedlings is stopped before domestication, the mandarin fish seedlings are transferred into a domestication pond containing sodium chloride or sodium sulfate and fed every other day, and mixed water is added into the domestication pond for domestication; the invention establishes the technology for domesticating and screening the mandarin seedlings suitable for the chloride-type and sulfate-type water bodies for the first time, has extremely strong pertinence, and can greatly improve the industrialization level of salt-resistant mandarin seedlings. The domestication technology has strong operability, so that the survival rate of the pond is improved, and the domestication cost is reduced; the screening technology is convenient and quick, and can be used for cultivating the mandarin salt-resistant variety or strain. Therefore, the method has the characteristics of strong pertinence, high survival rate, convenience and rapidness, and can meet the requirement of large-scale screening programs.
Description
Technical Field
The invention belongs to the technical field of aquaculture, and particularly relates to a salt-tolerant mandarin fish fry domestication method.
Background
The 6.9 hundred million mu of saline-alkali water in China has the characteristics of complex and various water chemical compositions, high ion coefficient, incapability of drinking by people and livestock and the like. Saline-alkali water is a rich unconventional homeland resource compared with a shortage of fresh water resources, but also puts higher demands on development and utilization. The main types of domestic saline-alkali water are as follows: chloride type, sulfate type, carbonate type and composite type, corresponding ion component is Cl - 、SO 4 2- 、HCO 3 - And mixing predominates. Wherein, when Cl in water body - When the blood pressure rises, physiological processes such as biological osmotic pressure regulation, endocrine balance, oxygen consumption and the like can be influenced. Water bodySO in (B) 4 2- Mainly from the dissolution of rock, fish lack active mechanisms for absorbing or regulating sulfate. Due to fish to Cl - 、SO 4 2- The response mechanisms of the water quality control method are different, so that the saline-alkali resistant economic varieties suitable for water bodies with different ion components need to be screened and cultivated, and a cultivation object is provided for constructing a cultivation mode suitable for water quality of a place. At present, the oasis fishery in inland saline-alkali water areas of China faces the outstanding problems of few suitable cultivation types, lack of saline-alkali resistant varieties and unstable cultivation yield, the economic types of high-quality aquatic products are taken as screening targets, and the establishment of new strains suitable for inland saline-alkali water areas cultivation by means of saline-alkali resistant environmental stress is a common technical method for solving the problems. The technical methods generally cultivate a saline-alkali tolerant family for the variety with improved variety potential, and carry out saline-alkali performance test, domestication and cultivation production pilot-scale test on the screened saline-alkali tolerant strain and main cultivation variety in typical saline-alkali water to obtain a cultivation strain suitable for a specific saline-alkali type. However, the technical methods have various problems, such as lack of integration of key technologies such as large-scale breeding of the saline-alkali resistant seeds, saline-alkali domestication of the seeds, efficient feeding, water environment maintenance and the like, no standardized cultivation technical system of the saline-alkali water of the seeds is formed, the industrialization level of the saline-alkali resistant seeds cannot be greatly improved, and in addition, the cultivation structure and management mode of the saline-alkali water area need to be further optimized.
The mandarin fish is a traditional rare freshwater fish in China, and has high economic value in cultivation. Mandarin fish is of meat type, and is mainly fed with bait fish, and the cultivation area is in south China, east China and China. At present, mandarin fish culture mainly comprises siniperca chuatsi (Siniperca chuatsi). The siniperca chuatsi grow fast, the current year cultivation can reach more than 500g, and the siniperca chuatsi has varieties of "Huakang No. 1", "Wu Nong No. 1" and the like; siniperca scherzeri (S scherzeri) is another cultivation type, the growth speed is low, and 100-150g of cultivation is carried out in the same year; there are 2 hybrid varieties of "autumn-Pu hybrid siniperca scherzeri" and "long-bead hybrid siniperca chuatsi". At present, all of these varieties are cultivated in fresh water. An acid-base tolerance experiment is carried out on mandarin fish seeds in the early stage of Shanghai ocean university, and mandarin fish has certain tolerance in the pH=5-9 range. Therefore, the mandarin fish variety domestication and screening cultivation tests are carried out in the saline-alkali water, so that a new cultivation variety can be provided for saline-alkali water cultivation, and meanwhile, the method has a great promotion effect on improving the saline-alkali soil cultivation benefit and long-term development.
Transplanting fish into a new water area different from the conventional condition, wherein the fish changes its morphological structure and physiological habit to adapt to the new environment condition to some extent is called domestication or domestication. The premise of successful domestication is to accurately grasp the proper time of the domestication, and the process of gradually changing the environment must be paid attention to in the domestication process, so that the domestication cannot be carried out in one step. For salinity domestication, most of the salinity domestication is gradually increased to target salinity. For example: the sea water domestication method of Acipenser sinensis, namely, raising the salinity of the culture water by 3-5 per mill every three days before 15 per mill; the salinity is 15-25 per mill, and the salinity is increased by 1-3 per mill every five days; after the salinity reaches 25 per mill, domesticating the Chinese sturgeon fries for 15-30 days, then raising the salinity by 1 per mill every three days, and transiting to the seawater with the salinity of 32 per mill (2004 of Chinese zodiac Zhonghong, etc.); the domestication method of the pure freshwater species of sturgeon with stepwise increasing salinity, namely, every increment of salinity is 4-6 per mill as one stage in the domestication process, every increment of 10-30% of the salinity of the stage every day in each stage, after finishing the domestication of the stage, the next stage of domestication is carried out for 2-4 days, the domestication is continued until the domestication salinity reaches the seawater salinity, and the general domestication survival rate is 35-76% (2005 such as lake light source, etc.); the single salinity domestication of the rainbow trout improves 2-8ppt per day, and the survival rate is 39-78% (Fei et al 2020); the individual salinity domestication of the tilapia is improved by 2-4ppt every day, and the survival rate is 61-97 percent (Zong Li, etc., 2003); the saline-alkali domestication of the prawns, the litopenaeus vannamei is subjected to ion domestication after the salinity of the litopenaeus vannamei is reduced by 1-5 per day, and the survival rate of the litopenaeus vannamei in the pond is 80% (2018 of Mingchen, and the like). In addition, the freshwater domestication method of the fragrant fish adopts a multi-generation and gradient salinity freshwater domestication method, and is matched with special baits to perform freshwater domestication on the fragrant fish, and the seventh-generation parent fish finally obtained through six-generation gradient domestication can be directly subjected to freshwater aquaculture (Ji Lianyun and the like 2020).
Phenotype screening is to screen out excellent characters expressed by plants in the breeding process of animals and plants, and finally, the excellent characters can be fixed on the plants, so that excellent varieties or strains can be cultivated. A rapid and easy method of assessing phenotypes is needed for animal and plant breeding processes so that the desired individuals are identified as early as possible in the screening process. Standard detection techniques, such as acute stress lethal experiments, have been used for phenotypic screening in the breeding process of varieties such as saline-alkali tolerant tilapia.
Mandarin is generally cultivated in fresh water, and a method for successfully domesticating and screening strains with different salinity (chloride type and sulfate type) of mandarin is not yet known.
Different kinds of fishes have large tolerance difference to saline-alkali environment stress, and different types of saline water bodies have different influences on fishes, so that no domestication and screening technology aiming at siniperca chuatsi in different types of saline water bodies exists at present. In addition, the prior fish saline-alkali resistant fingerling domestication technology has a plurality of defects, wherein the most important appearance is that the survival rate after domestication is low.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a salt-resistant mandarin fish fry domestication method suitable for chloride-type and sulfate-type water areas.
To achieve the above object, the solution of the present invention is:
a salt-tolerant mandarin fish fry domestication method comprises the following steps:
(1) Culturing mandarin fish fries in a fresh water pond, and feeding live baits every day to adapt the mandarin fish fries to the environment;
(2) Mixing high salinity water with fresh water to obtain mixed water;
(3) And (3) stopping feeding mandarin fish fries 24 hours before domestication, transferring the mandarin fish fries which are not fed into a domestication pond containing sodium chloride or sodium sulfate, feeding every other day, and adding the mixed water in the step (2) into the domestication pond for domestication.
Preferably, in step (1) and step (2), the conditions of the adapted environment and the mixing water are: the dissolved oxygen is 5.0-6.0mg/L, the water temperature is 24.6+ -0.5 ℃, and the pH is 7.5+ -0.1.
Preferably, in step (1), the live baits are selected from the group consisting of trichina or crucian.
Preferably, in step (2), the high salinity water is selected from natural brine with a sodium chloride concentration of 65%o or seawater with a sodium chloride concentration of 35%o.
Preferably, in step (2), the fresh water is selected from underground well water or aerated tap water.
Preferably, in the step (3), the salinity of the sodium chloride in the domestication pond is 4-13 per mill, and the salinity is increased by 3 per mill every 3 days.
Preferably, in step (3), the salinity of the sodium sulfate in the domestication pond is 2-11g/L, and the salinity is increased by 3g/L every 3 days.
By adopting the scheme, the invention has the beneficial effects that:
the method establishes the mandarin fish seed domestication and screening technology suitable for the chloride-type and sulfate-type water bodies for the first time, has extremely strong pertinence, and can greatly improve the industrialization level of salt-resistant mandarin fish seeds. The domestication technology has strong operability, improves the survival rate of the pond, and reduces the domestication period and the cost; the screening technology is convenient and quick, and can be used for cultivating the mandarin salt-resistant variety or strain. Therefore, the method has the characteristics of strong pertinence, high survival rate, convenience and rapidness, and can meet the requirement of large-scale screening programs.
Drawings
FIG. 1 is a flow chart of a mandarin fish fingerling domestication technology adapted to a sodium chloride-type water body in embodiment 1 of the present invention.
FIG. 2 is a graph showing the morphological changes of sodium chloride salinity-stressed siniperca gills tissue (a fresh water; b stress 12h; c fresh water recovery 24 h) in example 1 of the present invention.
FIG. 3 is a graph of regression curve equation of sodium chloride salinity versus cumulative mortality in example 1 of the present invention.
FIG. 4 is a flowchart of a mandarin fish fingerling domestication technique adapted to a sodium sulfate type water body in example 2 of the present invention.
FIG. 5 shows the sodium sulfate salt stress Siniperca Chuatsi structure morphology change pattern (a fresh water; b stress 12h; c fresh water recovery 24 h) in example 2 of the invention.
FIG. 6 is a graph of regression curve equation of sodium sulfate salinity versus cumulative mortality in example 2 of the present invention.
Detailed Description
The invention provides a salt-tolerant mandarin fish fry domestication method.
1. Through repeated fumbling and practical inspection, the invention provides a domestication scheme which is respectively suitable for two different water bodies of chloride type and sulfate type for siniperca chuatsi, relates to the specifications of domesticated fries, initial and final domestication concentrations, concentration improvement, feeding strategies during domestication and the like, and can obviously improve the adaptability of the siniperca chuatsi to the salinity environment through the scheme.
2. The invention refines screening conditions of siniperca chuatsi chloride-tolerant and sulfate-tolerant water bodies, such as concentration gradient setting, fish fry screening specification and the like, and can rapidly screen salt-tolerant individuals.
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments and the accompanying drawings. It will be apparent that the described embodiments are only some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1:
mandarin fish seed domestication and screening technology suitable for sodium chloride type water body
1. Seed preparation
Before mandarin fish fries are put into the fish pond, all fish ponds are disinfected, and proper bait fishes such as dace, crucian and the like are prepared. The mandarin fish fries are temporarily cultured in a fresh water pond, and the live baits are fed once a day and are sufficient. And (3) checking oxygenation conditions of the water body at regular time, keeping dissolved oxygen at 5.0-6.0mg/L, keeping the water temperature at 24.6+/-0.5 ℃ and the pH at 7.5+/-0.1, and carrying out domestication test after the fish completely adapt to the environment.
2. Water preparation
Is prepared by blending high salinity water (such as natural salt biogas water with salinity of 65 permillage, sea water of 35 permillage and the like) and fresh water (such as underground well water, tap water after full aeration and the like), or adding NaCl with corresponding proportion according to the volume of water, such as 4 permillage, namely adding 4g of salt into 1L of water, wherein the salinity of the salt water NaCl is 4 permillage. And (5) after the water quality is uniformly mixed, the mixture is used. The salinity is measured by a WYY-I type optical refraction salinity meter, and water quality indexes such as pH, dissolved oxygen, ammonia nitrogen, nitrite and the like are respectively tested by a kit.
3. Domestication technology
(1) Screening mandarin seedlings with similar specifications (about 5g in mass) from temporary rearing groups for salinity domestication, and stopping feeding in a fresh water pond 24 hours before domestication;
(2) Transferring the seedlings to a domestication pond with NaCl salinity of 4 per mill;
(3) Feeding no matter in the domestication pond on the 1 st day;
(4) Feeding on day 2 in the domestication pond;
(5) Feeding no matter in the domestication pond on the 3 rd day;
(6) Adding high salinity water prepared from natural or NaCl into the domestication pond to increase salinity gradient by 3%o (i.e. final concentration of 7%o);
repeating the steps (3) to (6) until the salinity is increased to 13 per mill, and after domestication, the survival rate is 60-80%. The flow is shown in fig. 1. And (5) timely fishing out dead fish in the domestication process, and cleaning and replacing the net cage periodically.
4. Screening technique
(1) And preliminarily knowing the tolerance of mandarin to NaCl salinity by utilizing histological observation. The mandarin fries are directly transferred into NaCl salinity water with salinity of 12 per mill from fresh water, transferred into fresh water for recovery after stress for 12h, and gill tissues of 3 test fishes are respectively collected for 12h before stress, after stress and 24h for recovery of fresh water and are fixed in a Boen, and paraffin slices are manufactured according to a conventional method and observed under a microscope. As a result, it was found that 12% sodium chloride salt stress affected gill morphology (FIG. 2).
(2) And calculating the 96-hour semi-lethal concentration of about 5g mandarin fish fries under NaCl salinity stress. The NaCl salinity stress gradient (0, 7 mill, 9 mill, 11 mill, 12 mill) was set. Firstly, temporarily raising fish in a control water body without environmental stress (for example, a tap water group with sufficient aeration is used as a control), then, directly putting test fish into water bodies with different environmental stresses, setting 3 fish in each group, and carrying out 96-hour acute stress experiments. After the start of the experiment, the death was recorded every 4h observation. The bottom of the static water tank of the test fish is used for stopping breathing, and no reaction is caused by touching with a glass rod as a death standard. The state is kept stable during the test, the feeding is not carried out, and the excrement is sucked out by a siphon pipe so as to keep the water clean. After the cumulative mortality is converted into probability units, a regression equation of NaCl salinity and the cumulative mortality is fitted by using a linear regression model, and the 96-hour semi-lethal concentration (LD 50) is obtained. Calculated is NaCl salinity stress ld50= 13.459%o (fig. 3).
(3) And (3) carrying out acute stress lethal screening according to the semi-lethal concentration set in the step (1), and eliminating individuals with poor tolerance to stress environment. Namely, mandarin fish fries with the mass of about 5g are directly transferred into 13%NaCl water from fresh water, and after 96 hours, survival individuals are reserved for further breeding, and dead individuals are eliminated, wherein the elimination rate is about 50%.
Example 2:
mandarin fish seed domestication and screening technology suitable for sodium sulfate type water body
1. Seed preparation
As in example 1.
2. Water preparation
Adding Na in corresponding proportion according to the volume of water 2 SO 4 Formulated as Na 2 SO 4 Salinity 2g/L, i.e. 1L water is added with 2g of Na 2 SO 4 The brine sulfate concentration was 2g/L.
3. Domestication technology
(1) Screening mandarin seedlings with similar specifications (about 5g in mass) from temporary rearing groups, performing sulfate salinity domestication, and stopping feeding in a fresh water pond 24 hours before domestication;
(2) Transferring the seedlings to Na 2 SO 4 2g/L domestication pond;
(3) Feeding no matter in the domestication pond on the 1 st day;
(4) Feeding on day 2 in the domestication pond;
(5) Feeding no matter in the domestication pond on the 3 rd day;
(6) Adding Na into domestication pond 2 SO 4 Formulated high salinity water to raise the salinity gradient by 3g/L (i.e., a final concentration of 5 g/L);
repeating the steps (3) to (6) until the salinity is increased to 11g/L, and after domestication, the survival rate is 60-80%. The flow is shown in fig. 4. And (5) timely fishing out dead fish in the domestication process, and cleaning and replacing the net cage periodically.
4. Screening technique
(1) Preliminary knowledge of Mandarin to Na by histological observation 2 SO 4 Tolerance to salinity. Transferring mandarin seedling directly from fresh water into Na with salinity of 14.7g/L 2 SO 4 In the salinity water body, after stress for 12 hours, fresh water recovery is carried out, gill tissues of 3 test fishes are respectively collected before stress, after stress for 12 hours and after fresh water recovery for 24 hours, and are put into a Barn for fixation, paraffin slices are manufactured according to a conventional method, and are observed under a light microscope. As a result, it was found that 14.7g/L of Na 2 SO 4 Salinity stress affected gill morphology (fig. 5).
(2) Calculation of Na 2 SO 4 The salinity stress is about 5g of mandarin fish fries at 96h semi-lethal concentration. Setting Na 2 SO 4 Salinity stress gradient (0, 7g/L, 9g/L, 11g/L, 12 g/L). Firstly, temporarily raising fish in a control water body without environmental stress (for example, a tap water group with sufficient aeration is used as a control), then, directly putting test fish into water bodies with different environmental stresses, setting 3 fish in each group, and carrying out 96-hour acute stress experiments. After the start of the test, the death was recorded every 4h observation. The bottom of the static water tank of the test fish is used for stopping breathing, and no reaction is caused by touching with a glass rod as a death standard. The state is kept stable during the test, the feeding is not carried out, and the excrement is sucked out by a siphon pipe so as to keep the water clean. After the cumulative mortality is converted into probability units, a linear regression model is used for fitting Na 2 SO 4 The regression equation of salinity and cumulative mortality, the 96h semi-lethal concentration (LD 50) was found. Calculating to obtain Na 2 SO 4 Salinity stress ld50= 11.286g/L (fig. 6).
(3) And (3) carrying out acute stress lethal screening according to the semi-lethal concentration set in the step (1), and eliminating individuals with poor tolerance to stress environment. Namely, mandarin fish fries with the body mass of about 5g are directly transferred into 11g/L Na from fresh water 2 SO 4 In the salinity water body, surviving individuals are reserved for the next breeding after 96 hours, and dead individuals are eliminated, wherein the elimination rate is about 50%.
The previous description of the embodiments is provided to facilitate a person of ordinary skill in the art in order to make and use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above-described embodiments. Those skilled in the art will appreciate that, in light of the principles of the present invention, improvements and modifications can be made without departing from the scope of the invention.
Claims (5)
1. A salt-tolerant mandarin fish fry domestication method is characterized in that: the method comprises the following steps:
(1) Culturing mandarin fish fries in a fresh water pond, and feeding live baits every day to adapt the mandarin fish fries to the environment; the mandarin fish fries are of siniperca chuatsi type;
(2) Adding prepared brine into a domestication pond, wherein the brine is sodium chloride brine with the salinity of 4 per mill or sodium sulfate brine with the salinity of 2 g/L;
(3) Stopping feeding mandarin fish fries 24 hours before domestication, transferring the mandarin fish fries which are not fed into a domestication pond containing sodium chloride or sodium sulfate, feeding every other day, and adding the salt water in the step (2) into the domestication pond for domestication; the salinity of sodium chloride in the domestication pond is 4-13 per mill, and the salinity is increased by 3 per mill every 3 days; alternatively, the salinity of the sodium sulfate in the domestication pond is 2-11g/L, and the salinity is increased by 3g/L every 3 days.
2. The method for domesticating salt-tolerant mandarin fish fries according to claim 1, wherein: in the step (1) and the step (2), the conditions of the adaptive environment and the saline water are as follows: the dissolved oxygen is 5.0-6.0mg/L, the water temperature is 24.6+ -0.5 ℃, and the pH is 7.5+ -0.1.
3. The method for domesticating salt-tolerant mandarin fish fries according to claim 1, wherein: in the step (1), the live baits are selected from dace or crucian.
4. The method for domesticating salt-tolerant mandarin fish fries according to claim 1, wherein: in the step (2), the sodium chloride brine is obtained by mixing high-salinity water and fresh water; the high-salinity water is selected from natural brine biogas water with the concentration of sodium chloride of 65 permillage or seawater with the concentration of sodium chloride of 35 permillage, and the fresh water is selected from underground well water or tap water after aeration.
5. The method for domesticating salt-tolerant mandarin fish fries according to claim 1, wherein: in the step (2), the sodium sulfate brine is added with Na in a corresponding proportion according to the volume of water 2 SO 4 Is prepared by the following steps.
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