CN103461243A - Low-salinity cultivating method for siniperca scherzeri steindachner fries - Google Patents

Low-salinity cultivating method for siniperca scherzeri steindachner fries Download PDF

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CN103461243A
CN103461243A CN2013104227325A CN201310422732A CN103461243A CN 103461243 A CN103461243 A CN 103461243A CN 2013104227325 A CN2013104227325 A CN 2013104227325A CN 201310422732 A CN201310422732 A CN 201310422732A CN 103461243 A CN103461243 A CN 103461243A
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salinity
mandarin fish
fed
spot mandarin
fry
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CN103461243B (en
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梁旭方
周怡
袁小琛
李艾璇
易提林
何珊
袁勇超
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Huazhong Agricultural University
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Abstract

The invention belongs to technical field of aquaculture, and particularly discloses a low-salinity cultivating method for siniperca scherzeri steindachner fries. The method comprises the following steps: adjusting the salinity of a siniperca scherzeri steindachner fry cultivating water body; increasing the salinity of the water body by 0.05-0.2 percent every day till the salinity is up to 0.3-0.6 percent; feeding baits once in the morning and at the night every day; controlling the water temperature at 25+/-1 DEG C and the pH value at 7.0-7.5 and keeping the dissolved oxygen in water more than or equal to 8mg/L, wherein the baits are young fairy shrimps which are fed in the amount of 3-7 pieces per milliliter and megalobrama amblycephala fries which are fed in the amount of 4-8 times that of the quantity of siniperca scherzeri steindachner fries. According to the method, the disease infection during a fry production period can be lowered, the survival rate of fry cultivation is increased, the death rate of the young fairy shrimps is lowered, and the utilization ratio of the baits is increased; the method has the characteristics of easiness and convenience in operating, easiness in popularizing, and the like.

Description

A kind of spot mandarin fish seed Low-salinity breeding method
Technical field
The invention belongs to technical field of aquaculture, be specifically related to a kind of spot mandarin fish seed Low-salinity breeding method.
Background technology
Mandarin fish is the famous and precious predacious fish of China's tradition, without stinging between flesh, and the plump delicacy of meat, delicious flavour is good to eat, is of high nutritive value.Wherein, spot mandarin fish (Siniperca scherzeri Steindachner), have the good reputation of " fresh water lithosporic ", liked by the consumer, is the important water outlet of China one of the fish of earning foreign exchange.The spot mandarin fish is subordinate to Perciformes mandarin fish subfamily, is the East Asia Endemic fish, China, and the Liaohe River, Hun River, Bi Liuhe, Yalu River water system and the Changjiang river on the south the Yellow River to each water system of the Zhujiang River all has distribution.
At present, what the mandarin fish seed produced and cultivation is all carried out under fresh water environment, have following problem: 1. artificial breeding and cultivation all easily cause many germ vertical infections, the death of fry susceptible disease under fresh water environment.2. mandarin fish is typical carnivorous predacious fish, take live fish and shrimp all the life as food, seed bait is mainly artemia larvae and forage fiss, the forage fiss cost is high, and artemia larvae can not be grown in pure fresh water, can be dead in general 2-3 hour, the spot mandarin fish halogen worm that death sinks to the bottom that do not ingest, cause the waste of bait, also can ruin water quality simultaneously.3. the seed production period need to often change water, consumes a large amount of high-quality freshwater resources.
Summary of the invention
The objective of the invention is for above-mentioned defect, a kind of spot mandarin fish seed Low-salinity breeding method is provided.
Above-mentioned purpose is achieved through the following technical solutions:
A kind of spot mandarin fish seed Low-salinity breeding method, the method is: adjust spot mandarin fish seedling and cultivate limnal salinity, make water salinity increase 0.5-2 ‰ every day, until salinity reaches 3-6 ‰, morning every day, evening are thrown something and fed bait once, controlling water temperature is 25 ± 1 ℃, the pH value is 7.0-7.5, keeps oxygen in water >=8mg/L, and described bait is the fairy shrimp young and megalobrama amblycephala fry, the described fairy shrimp young is thrown something and fed by 3-7/ml, and described megalobrama amblycephala fry is is doubly thrown something and fed by the 4-8 of spot mandarin fish seedling quantity.
Preferably, make water salinity increase by 1 ‰ every day, until salinity reaches 5 ‰.
Further preferably, the described fairy shrimp young is thrown something and fed by 5/ml, and described megalobrama amblycephala fry is thrown something and fed by 5 times of spot mandarin fish seedling quantity.
In the present invention, said salinity refers to the weight content of salt.
The invention has the beneficial effects as follows: the disease that 1. reduces the seed growing period infects, and has improved the survival rate of seed rearing; 2. reduce the lethality of the fairy shrimp young, improved the availability of bait; 3. can use natural brackish water to regulate the salt content of water, thereby save clean freshwater resources, reduce production costs.The present invention is easy and simple to handle, is easy to promote.
The accompanying drawing explanation
Fig. 1 is spot mandarin fish somatotropin GH(Growth hormone under different salinity) relative expression quantity;
Fig. 2 is spot mandarin fish quasi-insulin growthing factor I GF(Insulin-like growth factor under different salinity) relative expression quantity;
Fig. 3 is spot mandarin fish fatty acid desaturase FAD(Fatty acid desaturase under different salinity) relative expression quantity;
Fig. 4 is spot mandarin fish fatty acid prolonging enzyme ELO(Fatty acid elongase under different salinity) relative expression quantity;
Fig. 5 is spot mandarin fish hepatic lipase HL(Hepatic lipase under different salinity) relative expression quantity;
Fig. 6 is spot mandarin fish hormone-sensitive lipase HSL(Hormone-sensitive lipase under different salinity) relative expression quantity;
Fig. 7 is the different bait spot mandarin fish somatotropin GH relative expression quantities of feeding under 5 ‰ Variation of Salinity Conditions;
Fig. 8 is the different bait spot mandarin fish quasi-insulin growthing factor I GF relative expression quantities of feeding under 5 ‰ Variation of Salinity Conditions;
Fig. 9 is the different bait spot mandarin fish fatty acid desaturase FAD relative expression quantities of feeding under 5 ‰ Variation of Salinity Conditions;
Figure 10 is the different bait spot mandarin fish fatty acid prolonging enzyme ELO relative expression quantities of feeding under 5 ‰ Variation of Salinity Conditions;
Figure 11 is the different bait spot mandarin fish hepatic lipase HL relative expression quantities of feeding under 5 ‰ Variation of Salinity Conditions;
Figure 12 is the different bait spot mandarin fish hormone-sensitive lipase HSL relative expression quantities of feeding under 5 ‰ Variation of Salinity Conditions.
In figure, different letters represent significant difference, P<0.05.
Embodiment
Below in conjunction with embodiment, the present invention is described in detail.
Embodiment 1
Implement place: in great Bao fishing ground, Fengcheng City, Liaoning Province hatching booth, step is as follows:
1) fry and feed source: Yalu River spot mandarin fish seedling be with a collection of after numerous rupture of membranes the healthy fries of 5 ages in days; Forage fiss megalobrama amblycephala seedling is the healthy fry of 3 ages in days after numerous rupture of membranes; The fairy shrimp young obtains for buying good year ovum hatching.
2) aquarium (150cm * 55cm * 50cm), clean with 10mg/L disinfecting solution of potassium permanganate afterflush before test, then fill fresh water.
3) put seedling: selective body is strong without blemish mandarin fish spray (1cm), and each aquarium is put seedling 100 tails.
4) salinity is regulated: put seedling and started progressively to add natural brackish water (salinity is greater than 5 ‰) same day and improve salinity, make every day water salinity increase by 1 ‰, until salinity reaches 5 ‰.
5) bait feeding: throw something and feed the open-mouthed bait fairy shrimp young and megalobrama amblycephala fry, 5:00 and late 8:00 threw and raised 2 times morning every day, and the feeding volume of the fairy shrimp young is 5/ml; The megalobrama amblycephala feeding volume is 5 times of spot mandarin fish seed quantity.
6) daily management: 25 ± 1 ℃ of water temperatures, pH7.0-7.5, observe the fish state every day, records natural death spot mandarin fish mantissa, removes bottom ight soil and swill, changes water 2/3, and blowing aeration keeps oxygen in water >=8mg/L.
Embodiment 2
Method is identical with embodiment 1, and difference is: salinity in water is increased to 1 ‰ every day, until salinity reaches 10 ‰.
Through cultivation in 15 days, spot mandarin fish growth result was in Table 1:
Figure BDA0000382783860000041
Different letters represent significant difference, P<0.05.
As can be seen from the above results: increase by 1 ‰ by salinity every day, and salinity is adjusted to 5 ‰, and the growing state of spot mandarin fish seedling is best, and body weight and survival rate are the highest.
Adopt the Trizol method to extract Comparative Examples and the total RNA of embodiment 1,2 full fish, take cDNA after reverse transcription as template, the expression of quantitative PCR analysis growth and metabolism related gene, correlated results is shown in accompanying drawing 1-6.Gene expression pattern is from molecular level reaction fish body physiological status and Regulation Mechanism, from accompanying drawing 1-6, under 5 ‰ Variation of Salinity Conditions, the gene high expression of somatotropin GH, these two growth correlations of quasi-insulin growthing factor I GF, Genes Associated with Lipid Metabolism fatty acid desaturase FAD, fatty acid prolonging enzyme ELO, hepatic lipase HL and hormone-sensitive lipase HSL expression are also the highest simultaneously, this shows that 5 ‰ Variation of Salinity Conditions promote the expression of spot mandarin fish seedling metabolism and growth hormone gene, thereby promotes the growth of spot mandarin fish.
Embodiment 3
Implement place: in great Bao fishing ground, Fengcheng City, Liaoning Province hatching booth, experimental procedure comprises:
1) fry and feed source: Yalu River spot mandarin fish seedling be with a collection of after numerous rupture of membranes the healthy fries of 5 ages in days; Forage fiss megalobrama amblycephala seedling is the healthy fry of 3 ages in days after numerous rupture of membranes; The fairy shrimp young obtains for buying good year ovum hatching.
2) aquarium (150cm * 55cm * 50cm), clean with 10mg/L disinfecting solution of potassium permanganate afterflush before test, then fill fresh water.
3) put seedling: selective body is strong without blemish mandarin fish spray (1cm), and each aquarium is put seedling 100 tails.
4) salinity is regulated: put seedling and started progressively with salt, to improve salinity the same day, make every day water salinity increase by 1 ‰, until salinity reaches 5 ‰.
5) bait feeding: divide 3 groups, throw something and feed respectively the open-mouthed bait fairy shrimp young (A group), megalobrama amblycephala fry (B group) and the fairy shrimp young+megalobrama amblycephala fry (C group), early 5:00 and late 8:00 threw and raised 2 times every day, and the A group fairy shrimp young of throwing something and feeding keeps 10/ml; The B group megalobrama amblycephala feeding volume of throwing something and feeding is 8 times of spot mandarin fish seed quantity; C group fairy shrimp is thrown something and fed and keeps 5/ml, and the megalobrama amblycephala feeding volume is 5 times of spot mandarin fish seed quantity.
6) daily management: 25 ± 1 ℃ of water temperatures, pH7.0-7.5, observe the fish state every day, records natural death spot mandarin fish mantissa, removes bottom ight soil and swill, changes water 2/3, and blowing aeration keeps oxygen in water >=8mg/L.
Through cultivation in 15 days, spot mandarin fish growth result saw the following form:
Different letters represent significant difference, P<0.05.
Adopt the Trizol method to extract the total RNA of full fish, take cDNA after reverse transcription as template, the expression of quantitative PCR analysis growth and metabolism related gene, correlated results is shown in accompanying drawing 7-12.From accompanying drawing 7-12, under 5 ‰ Variation of Salinity Conditions, C group (the fairy shrimp young+blunt-snout gurnards seedling combined bait) and (megalobrama amblycephala fry group) the spot mandarin fish seedling metabolism of B group and growth hormone gene are expressed basically identical, and be significantly higher than A group (the fairy shrimp young throw something and feed group), this is consistent with growth result, show under 5 ‰ Variation of Salinity Conditions, the suitableeest bait of spot mandarin fish seed is that fairy shrimp+megalobrama amblycephala fry combination is thrown something and fed.

Claims (3)

1. a spot mandarin fish seed Low-salinity breeding method, it is characterized in that: adjust spot mandarin fish seedling and cultivate limnal salinity, make water salinity increase 0.5-2 ‰ every day, until salinity reaches 3-6 ‰, then morning every day, evening throw something and feed bait once, controlling water temperature is 25 ± 1 ℃, the pH value is 7.0-7.5, keeps oxygen in water >=8mg/L, and described bait is the fairy shrimp young and megalobrama amblycephala fry, the described fairy shrimp young is thrown something and fed by 3-7/ml, and described megalobrama amblycephala fry is is doubly thrown something and fed by the 4-8 of spot mandarin fish seedling quantity.
2. spot mandarin fish seed Low-salinity breeding method according to claim 1, is characterized in that: make water salinity increase by 1 ‰ every day, until salinity reaches 5 ‰.
3. spot mandarin fish seed Low-salinity breeding method according to claim 1, it is characterized in that: the described fairy shrimp young is thrown something and fed by 5/ml, and described megalobrama amblycephala fry is thrown something and fed by 5 times of spot mandarin fish seedling quantity.
CN201310422732.5A 2013-09-16 2013-09-16 Low-salinity cultivating method for siniperca scherzeri steindachner fries Expired - Fee Related CN103461243B (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112154946A (en) * 2020-11-05 2021-01-01 华中农业大学 Method for cultivating indoor controllable inactivated bait fish with initial fish fries
CN113796340A (en) * 2021-10-12 2021-12-17 集美大学 Method for breeding micropterus salmoides
CN115152670A (en) * 2022-08-17 2022-10-11 上海海洋大学 Domestication method of salt-tolerant mandarin fish fries

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112154946A (en) * 2020-11-05 2021-01-01 华中农业大学 Method for cultivating indoor controllable inactivated bait fish with initial fish fries
CN112154946B (en) * 2020-11-05 2022-03-08 华中农业大学 Method for cultivating indoor controllable inactivated bait fish with initial fish fries
CN113796340A (en) * 2021-10-12 2021-12-17 集美大学 Method for breeding micropterus salmoides
CN115152670A (en) * 2022-08-17 2022-10-11 上海海洋大学 Domestication method of salt-tolerant mandarin fish fries
CN115152670B (en) * 2022-08-17 2024-03-29 上海海洋大学 Domestication method of salt-tolerant mandarin fish fries

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