CN103461243B - Low-salinity cultivating method for siniperca scherzeri steindachner fries - Google Patents
Low-salinity cultivating method for siniperca scherzeri steindachner fries Download PDFInfo
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- CN103461243B CN103461243B CN201310422732.5A CN201310422732A CN103461243B CN 103461243 B CN103461243 B CN 103461243B CN 201310422732 A CN201310422732 A CN 201310422732A CN 103461243 B CN103461243 B CN 103461243B
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- salinity
- mandarin fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention belongs to technical field of aquaculture, and particularly discloses a low-salinity cultivating method for siniperca scherzeri steindachner fries. The method comprises the following steps: adjusting the salinity of a siniperca scherzeri steindachner fry cultivating water body; increasing the salinity of the water body by 0.05-0.2 percent every day till the salinity is up to 0.3-0.6 percent; feeding baits once in the morning and at the night every day; controlling the water temperature at 25+/-1 DEG C and the pH value at 7.0-7.5 and keeping the dissolved oxygen in water more than or equal to 8mg/L, wherein the baits are young fairy shrimps which are fed in the amount of 3-7 pieces per milliliter and megalobrama amblycephala fries which are fed in the amount of 4-8 times that of the quantity of siniperca scherzeri steindachner fries. According to the method, the disease infection during a fry production period can be lowered, the survival rate of fry cultivation is increased, the death rate of the young fairy shrimps is lowered, and the utilization ratio of the baits is increased; the method has the characteristics of easiness and convenience in operating, easiness in popularizing, and the like.
Description
Technical field
The invention belongs to technical field of aquaculture, be specifically related to a kind of spot mandarin fish seed Low-salinity breeding method.
Background technology
The famous and precious predacious fish of Gui Shi China tradition, sting without between flesh, the plump delicacy of meat, delicious flavour is good to eat, is of high nutritive value.Wherein, spot mandarin fish (Siniperca scherzeri Steindachner), have the good reputation of " fresh water lithosporic ", deeply likes by consumer, is that the important water outlet of China is earned foreign exchange one of fish.Spot mandarin fish is subordinate to Perciformes mandarin fish subfamily, is East Asia Endemic fish, China, and all there is distribution in the Changjiang river on the south the Liaohe River, Hun River, Bi Liuhe, Yalu River water system and the Yellow River to each water system of the Zhujiang River.
At present, mandarin fish Seedling production and cultivation are all carried out under fresh water environment, there is following problem: 1. artificial breeding and cultivation all easily cause many germ vertical infections under fresh water environment, and fry susceptible disease is dead.2. mandarin fish is typical carnivorous predacious fish, lifelong take live fish and shrimp as food, seed bait is artemia larvae and forage fiss mainly, forage fiss cost is high, and artemia larvae can not be grown in pure fresh water, can be dead in general 2-3 hour, spot mandarin fish does not ingest the halogen worm that death sinks to the bottom, cause the waste of bait, also can ruin water quality simultaneously.3. need during Seedling production often to change water, consume a large amount of high-quality freshwater resources.
Summary of the invention
The object of the invention is for above-mentioned defect, a kind of spot mandarin fish seed Low-salinity breeding method is provided.
Above-mentioned purpose is achieved through the following technical solutions:
A kind of spot mandarin fish seed Low-salinity breeding method, the method is: adjustment spot mandarin fish seedling cultivates limnal salinity, make water salinity increase 0.5-2 ‰ every day, until salinity reaches 3-6 ‰, morning every day, evening throw something and feed bait once, controlling water temperature is 25 ± 1 DEG C, pH value is 7.0-7.5, and keep oxygen in water >=8mg/L, described bait is the fairy shrimp young and megalobrama amblycephala fry, the described fairy shrimp young is thrown something and fed by 3-7/ml, and described megalobrama amblycephala fry is is doubly thrown something and fed by the 4-8 of spot mandarin fish seedling quantity.
Preferably, water salinity is made to increase by 1 ‰ every day, until salinity reaches 5 ‰.
Further preferably, the described fairy shrimp young is thrown something and fed by 5/ml, and described megalobrama amblycephala fry is thrown something and fed by 5 times of spot mandarin fish seedling quantity.
In the present invention, said salinity refers to the weight content of salt.
The invention has the beneficial effects as follows: the disease infection 1. reducing seed growing period, improves the survival rate of seed rearing; 2. reduce the lethality of the fairy shrimp young, improve the availability of bait; 3. natural brackish water can be used to regulate the salt content of water, thus save clean freshwater resources, reduce production cost.The present invention is easy and simple to handle, is easy to promote.
Accompanying drawing explanation
Fig. 1 is spot mandarin fish somatotropin GH(Growth hormone under different salinity) relative expression quantity;
Fig. 2 is spot mandarin fish quasi-insulin growthing factor I GF(Insulin-like growthfactor under different salinity) relative expression quantity;
Fig. 3 is spot mandarin fish fatty acid desaturase FAD(Fatty acid desaturase under different salinity) relative expression quantity;
Fig. 4 is spot mandarin fish fatty acid elongase ELO(Fatty acid elongase under different salinity) relative expression quantity;
Fig. 5 is spot mandarin fish hepatic lipase HL(Hepatic lipase under different salinity) relative expression quantity;
Fig. 6 is spot mandarin fish hormone-sensitive lipase HSL(Hormone-sensitive lipase under different salinity) relative expression quantity;
Fig. 7 is different bait spot mandarin fish somatotropin GH relative expression quantity of feeding under 5 ‰ Variation of Salinity Conditions;
Fig. 8 is different bait spot mandarin fish quasi-insulin growthing factor I GF relative expression quantity of feeding under 5 ‰ Variation of Salinity Conditions;
Fig. 9 is different bait spot mandarin fish fatty acid desaturase FAD relative expression quantity of feeding under 5 ‰ Variation of Salinity Conditions;
Figure 10 is different bait spot mandarin fish fatty acid elongase ELO relative expression quantity of feeding under 5 ‰ Variation of Salinity Conditions;
Figure 11 is different bait spot mandarin fish hepatic lipase HL relative expression quantity of feeding under 5 ‰ Variation of Salinity Conditions;
Figure 12 is different bait spot mandarin fish hormone-sensitive lipase HSL relative expression quantity of feeding under 5 ‰ Variation of Salinity Conditions.
In figure, different letter represents significant difference, P<0.05.
Embodiment
Below in conjunction with embodiment, the present invention is described in detail.
Embodiment 1
Implement place: in great Bao fishing ground, Fengcheng City of Liaoning Province hatching booth, step is as follows:
1) fry and feed source: Yalu River spot mandarin fish seedling be with a collection of after numerous rupture of membranes the healthy fries of 5 ages in days; Forage fiss megalobrama amblycephala seedling is the healthy fry of 3 ages in days after numerous rupture of membranes; The fairy shrimp young obtains for buying good year egg hatching.
2) aquarium (150cm × 55cm × 50cm), clean with 10mg/L disinfecting solution of potassium permanganate afterflush before test, then fill fresh water.
3) seedling is put: selective body is strong without blemish mandarin fish spray (1cm), and each aquarium puts seedling 100 tail.
4) salinity regulates: put seedling and to start progressively to add natural brackish water (salinity is greater than 5 ‰) same day and improve salinity, make every day water salinity increase by 1 ‰, until salinity reaches 5 ‰.
5) bait feeding: throw something and feed the open-mouthed bait fairy shrimp young and megalobrama amblycephala fry, every day, early 5:00 and late 8:00 throwing raised 2 times, and the feeding volume of the fairy shrimp young is 5/ml; Megalobrama amblycephala feeding volume is 5 times of spot mandarin fish seed quantity.
6) daily management: water temperature 25 ± 1 DEG C, pH7.0-7.5, observes fish state every day, and record natural death Ban Gui mantissa, removes bottom ight soil and swill, change water 2/3, blowing aeration, keep oxygen in water >=8mg/L.
Embodiment 2
Method is identical with embodiment 1, and difference is: salinity in water is increased by 1 ‰, until salinity reaches 10 ‰ every day.
Cultivated through 15 days, spot mandarin fish growth result is in table 1:
Different letter represents significant difference, P<0.05.
As can be seen from the above results: every day by salinity increase by 1 ‰, salinity is adjusted to 5 ‰, and the growing state of spot mandarin fish seedling is best, body weight and survival rate the highest.
Adopting Trizol method to extract comparative example and the full fish total serum IgE of embodiment 1,2, is template with cDNA after reverse transcription, and the expression of quantitative PCR analysis growth and metabolism related gene, correlated results is shown in accompanying drawing 1-6.Gene expression pattern is from molecular level reaction fish body physiological status and Regulation Mechanism, from accompanying drawing 1-6, under 5 ‰ Variation of Salinity Conditions, the gene high expression that somatotropin GH, quasi-insulin growthing factor I GF these two growth is relevant, Genes Associated with Lipid Metabolism fatty acid desaturase FAD, fatty acid elongase ELO, hepatic lipase HL and hormone-sensitive lipase HSL expression are also the highest simultaneously, this shows that 5 ‰ Variation of Salinity Conditions promote the expression of spot mandarin fish seedling metabolism and growth hormone gene, thus promotes the growth of spot mandarin fish.
Embodiment 3
Implement place: in great Bao fishing ground, Fengcheng City of Liaoning Province hatching booth, experimental procedure comprises:
1) fry and feed source: Yalu River spot mandarin fish seedling be with a collection of after numerous rupture of membranes the healthy fries of 5 ages in days; Forage fiss megalobrama amblycephala seedling is the healthy fry of 3 ages in days after numerous rupture of membranes; The fairy shrimp young obtains for buying good year egg hatching.
2) aquarium (150cm × 55cm × 50cm), clean with 10mg/L disinfecting solution of potassium permanganate afterflush before test, then fill fresh water.
3) seedling is put: selective body is strong without blemish mandarin fish spray (1cm), and each aquarium puts seedling 100 tail.
4) salinity regulates: put seedling and to start the same day progressively to improve salinity with salt, make every day water salinity increase by 1 ‰, until salinity reaches 5 ‰.
5) bait feeding: divide 3 groups, to throw something and feed respectively the open-mouthed bait fairy shrimp young (A group), megalobrama amblycephala fry (B group) and the fairy shrimp young+megalobrama amblycephala fry (C group), early 5:00 and late 8:00 threw and raised 2 times every day, and the A group fairy shrimp young of throwing something and feeding keeps 10/ml; B group megalobrama amblycephala feeding volume of throwing something and feeding is 8 times of spot mandarin fish seed quantity; C group fairy shrimp is thrown something and fed maintenance 5/ml, and megalobrama amblycephala feeding volume is 5 times of spot mandarin fish seed quantity.
6) daily management: water temperature 25 ± 1 DEG C, pH7.0-7.5, observes fish state every day, and record natural death Ban Gui mantissa, removes bottom ight soil and swill, change water 2/3, blowing aeration, keep oxygen in water >=8mg/L.
Cultivated through 15 days, spot mandarin fish growth result sees the following form:
Different letter represents significant difference, P<0.05.
Adopting Trizol method to extract full fish total serum IgE, is template with cDNA after reverse transcription, and the expression of quantitative PCR analysis growth and metabolism related gene, correlated results is shown in accompanying drawing 7-12.From accompanying drawing 7-12, under 5 ‰ Variation of Salinity Conditions, C group (the fairy shrimp young+blunt-snout gurnards seedling combined bait) and (megalobrama amblycephala fry group) the spot mandarin fish seedling metabolism of B group and growth hormone gene are expressed basically identical, and be significantly higher than A group (the fairy shrimp young throw something and feed group), this is consistent with growth result, show under 5 ‰ Variation of Salinity Conditions, the suitableeest bait of spot mandarin fish seed is that fairy shrimp+megalobrama amblycephala fry combination is thrown something and fed.
Claims (1)
1. a spot mandarin fish seed Low-salinity breeding method, it is characterized in that: adjustment spot mandarin fish seedling cultivates limnal salinity, make water salinity increase by 1 ‰ every day, until salinity reaches 5 ‰, then morning every day, evening throw something and feed bait once, controlling water temperature is 25 ± 1 DEG C, pH value is 7.0-7.5, and keep oxygen in water >=8mg/L, described bait is the fairy shrimp young and megalobrama amblycephala fry, the described fairy shrimp young is thrown something and fed by 5/ml, and described megalobrama amblycephala fry is thrown something and fed by 5 times of spot mandarin fish seedling quantity.
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| CN112154946B (en) * | 2020-11-05 | 2022-03-08 | 华中农业大学 | Method for cultivating indoor controllable inactivated bait fish with initial fish fries |
| CN113796340B (en) * | 2021-10-12 | 2022-11-22 | 集美大学 | Method for breeding micropterus salmoides |
| CN115152670B (en) * | 2022-08-17 | 2024-03-29 | 上海海洋大学 | Domestication method of salt-tolerant mandarin fish fries |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1846481A (en) * | 2006-05-12 | 2006-10-18 | 陈纪明 | Mandarin cultivating method in pond |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1846481A (en) * | 2006-05-12 | 2006-10-18 | 陈纪明 | Mandarin cultivating method in pond |
Non-Patent Citations (6)
| Title |
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| Effects of the timing of initial feeding on growth and survival of spotted mandarin fish siniperca scherzeri lavae;L.Zhang 等;《Journal of fish biology》;20091009;第75卷(第6期);摘要 * |
| 余燕.点带石斑鱼(Epinephelus malabaricus)盐度适应和营养调控的研究.《中国优秀硕士学位论文全文数据库(电子期刊)》.2013,摘要. * |
| 斑鳜健康养殖技术开发;陈英波 等;《科学养鱼》;20130430(第4期);第35-36页 * |
| 李红敬 等.斑鳜池塘高效养殖技术.《信阳师范学院学报:自然科学版》.2011,第24卷(第3期),第397-399页. * |
| 盐度渐变和骤变对褐点石斑鱼存活和摄饵的影响;施钢 等;《广东海洋大学学报》;20110228;第31卷(第1期);第45-47页 * |
| 长江斑鳜养殖六要点;戴秀萍;《渔业致富指南》;20130731(第14期);第51页 * |
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