CN103387289B - Method for strengthening azo dye biodegradation by utilizing zero-valent iron - Google Patents

Method for strengthening azo dye biodegradation by utilizing zero-valent iron Download PDF

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CN103387289B
CN103387289B CN201310345316.XA CN201310345316A CN103387289B CN 103387289 B CN103387289 B CN 103387289B CN 201310345316 A CN201310345316 A CN 201310345316A CN 103387289 B CN103387289 B CN 103387289B
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valent iron
zero
azo dye
anoxic
reactor
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CN103387289A (en
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许玫英
周庆
方云
陈杏娟
孙国萍
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Institute of Microbiology of Guangdong Academy of Sciences
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Guangdong Institute of Microbiology
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Abstract

The invention discloses a method for strengthening azo dye biodegradation by utilizing zero-valent iron. The method comprises the following steps of: adding zero-valent iron into an active-sludge containing reactor for treating the azo dye wastewater; and realizing the azo dye biodegradation by adopting an anoxic-aerobiotic-anoxic sequencing type reactor process. According to the method, the anoxic-aerobiotic-anoxic sequencing type reactor process is adopted, and azo-containing wastewater and zero-valent iron are added, so that on one hand, zero-valent iron is used for chemical reduction of the azo dye at a first anoxic stage, on the other hand, zero-valent iron is used as an electron donor for promoting the microorganism growth, and therefore, the azo dye is catalyzed for being reduced and degraded into an aromatic compound; at an aerobiotic stage, the aerobiotic microorganisms are used for oxidizing and mineralizing the aromatic compound; meanwhile, the generated Fe<2+> is oxidized into Fe<3+>; at a second anoxic stage, generated Fe<3+> is used as an electron mediator or an electron receptor, so that the reduction activity of the microorganisms is promoted to be recovered, and COD (Chemical Oxygen Demand) is further removed, and therefore, preparation is made for taking in the fresh azo dye wastewater.

Description

One utilizes Zero-valent Iron to strengthen the biodegradable method of azoic dyestuff
Technical field
The invention belongs to biological technical field, be specifically related to one and utilize Zero-valent Iron to strengthen the biodegradable method of azoic dyestuff.
Background technology:
Along with the development of modern chemical industry, dyestuff is widely used in dyeing and the stamp process of multiple industry, and the dyeing waste water that the whole world is discharged every year about has 280000 tons.Wherein the usage quantity of azoic dyestuff is maximum, and the synthetic dyestuff of about 70% are made based on azo chemistry.The azoic dyestuff of current use reaches kind more than 3000, it is all almost artificial sintetics, its chemical stability is high, comparatively difficult for biological degradation, and most azoic dyestuff and metabolic intermediate thereof all have strong mutagenicity, carinogenicity, teratogenecity and other toxicity, be directly thrown in water body and will constitute a threat to ecotope, HUMAN HEALTH is worked the mischief.
For the characteristic of azoic dyestuff, existing treatment process has Physical, chemical method, biological process etc.Wherein biological restoration and more and more paying close attention to by people with its environment friendly, low cost, efficiency high with the treatment process that other technologies are used in conjunction.Iron is the element of the 4th most enrichment in the earth's crust, and it is present in the middle of environment with 0 ,+2 ,+3 valence state forms.Zero-valent Iron (ZVI) reductive action can facilitate azoic dyestuff deoxidization, degradation, but but can not realize the thorough mineralising of azoic dyestuff.The iron-reducing bacteria simultaneously existed in environment under anaerobic, can utilize Fe 3+as electron acceptor(EA), realize the conversion of azoic dyestuff, but microbial reaction speed is subject to the restriction of the conditions such as oxygen environment, dye toxicity.
Therefore, the biologic treating technique setting up azoic dyestuff fast degradation becomes the technological difficulties in research.
Summary of the invention:
The object of this invention is to provide a kind of Zero-valent Iron that utilizes that is quick, simple and easy, low cost and strengthen the biodegradable method of azoic dyestuff, to realize the fast degraded biologically of azoic dyestuff.
The Zero-valent Iron that utilizes of the present invention strengthens the biodegradable method of azoic dyestuff, it is characterized in that, comprises the following steps:
Zero-valent Iron is added process azo dye wastewater containing active sludge reactor in, adopt anaerobic-aerobic-anoxic sequencing batch reactor technique, realize azoic dyestuff biological degradation.
The content of described Zero-valent Iron is preferably 6mmolL -1.
Described anaerobic-aerobic-anoxic sequencing batch reactor technique, hydraulic detention time is 48h: wherein anoxic section 24h, aerobic section 12h, anoxic section 10h, idle 2h.
The present invention adopts anaerobic-aerobic-anoxic sequence batch (reaction process, adds containing dye wastewater and Zero-valent Iron, at first anoxic phases, Zero-valent Iron is chemical reduction azoic dyestuff on the one hand, on the other hand as electron donor, accelerate growth of microorganism, catalysis azoic dyestuff deoxidization, degradation is aromatic compound; Aerobic stage, aerobic microbiological completes oxidation, the mineralising of aromatic compound, the Fe simultaneously generated 2+be oxidized to Fe 3+; Second anoxic phases, the Fe of generation 3+as electron shuttle body or electron acceptor(EA), promote the recovery of the reducing activity of microorganism, and remove COD further, for fresh azo dye wastewater water inlet is prepared.The fast degradation and the COD that present invention achieves azoic dyestuff remove, for the removal technique of azoic dyestuff and organic pollutant provides science reference.
Accompanying drawing illustrates:
Fig. 1 is Zero-valent Iron-anaerobic-aerobic-anoxic sequencing batch reactor schema;
Fig. 2 is the impact of Zero-valent Iron on the removal ability of reactor amaranth;
Fig. 3 is the impact of Zero-valent Iron on reactor COD high loading water outlet pH.
Embodiment:
Following examples further illustrate of the present invention, instead of to control of the present invention.
Embodiment 1: add the impact that Zero-valent Iron is removed reactor amaranth
The azo dye wastewater of artificial preparation is as reaction water inlet.With sucrose-C, (NH4) 2sO 4-N, KH 2pO 4-P is as carbon source, nitrogenous source and phosphorus source, and its COD:N:P ratio is 200:5:1, and initial influent COD is 500mg/L; Trace element is CoCl 2.6H 2o:0.5gL -1, NiCl.6H 2o:0.05gL -1, CuSO 4.5H 2o:0.0465gL -1, ZnSO 4: 0.0893gL -1, H 3bO 3: 0.05gL -1, MnCl 2.4H 2o:0.5gL -1, (NH 4) 6mo 7o 24.2H 2o:0.01gL -1; Amaranth concentration is from 0.025mmolL -1progressively rise to 0.4mmolL -1, pH8.0, surplus is water.Each amaranth concentration gradient is shown in Fig. 2 working time.Adopt anaerobic-aerobic-anoxic sequencing batch reactor technique, processing parameter: hydraulic detention time is 48h: anaerobism section 24h, aerobic section 12h, anaerobism section 10h, idle 2h, the flooding quantity in each cycle is 1700mL.The amount of oxygen of aerobic section maintains 8-10mgL -1between.The flow process of Fe forms reduction reactor: intake and add Zero-valent Iron → anoxic → aerobic-anoxic water outlet, these processes are all carry out in same reactor, and schema is shown in Fig. 1.This experimental study adds the impact on amaranth clearance of Zero-valent Iron in the reactor.A reactor R1 inoculation of activated-sludge, basis adds Zero-valent Iron to reactor R2 inoculation of activated-sludge, and dosage is 6mmolL -1.Amaranth clearance calculates with the reduction of unit time (2 days) interior 520nm light absorption value, the results are shown in Figure 2.As figure shows, its decolorizing efficiency of the relative R1 of reactor R2 containing Zero-valent Iron significantly improves.Concrete analysis, when amaranth influent concentration is 0.025mmol/L-0.1mmol/L, the decolorizing efficiency of reactor R1, R2 does not have significant difference, when amaranth concentration brings up to 0.2mmol/L-0.4mmol/L, its decolorizing efficiency of reactor R2 relative response device R1 significantly improves, and more stable.Therefore, Zero-valent Iron add the decolouring being conducive to amaranth.
Embodiment 2: add Zero-valent Iron to the impact of water outlet pH under reactor high loading COD condition
Artificial distribution fills a prescription, (a reactor R1 inoculation of activated-sludge, basis adds Zero-valent Iron to reactor R2 inoculation of activated-sludge to reactor design, and dosage is 6mmolL -1), operating parameter with in embodiment 1, when running 38 days, influent COD is adjusted to 1000mg/L from 500mg/L, compares and adds part iron and changing without the water outlet pH of zero-valent iron reactor.As shown in Figure 3, when COD is 500mg/L, reactor R1, R2 water outlet pH is 85% at about 7.0, COD clearance to result.When COD is increased to 1000mg/L, reactor water outlet is all acid, and reactor R1 water outlet pH is 3.3-5.0, and reactor R2 water outlet pH is 5.4-6.8.Measure the COD in now aqueous solution, the COD clearance of reactor R1, reactor R2 is respectively 40%, 45% simultaneously.This shows that reactor R2 relative response device R1 can better maintain system stability under high loading COD condition.

Claims (2)

1. one kind utilizes Zero-valent Iron to strengthen the biodegradable method of azoic dyestuff, it is characterized in that, comprise the following steps: Zero-valent Iron is added process azo dye wastewater containing in the reactor of active sludge, adopt anerobic-aerobic-anerobic sequencing batch reactor technique, realize azoic dyestuff biological degradation;
Described anerobic-aerobic-anerobic sequencing batch reactor technique, hydraulic detention time is 48h: wherein anaerobism section 24h, aerobic section 12h, anaerobism section 10h, idle 2h.
2. the Zero-valent Iron that utilizes according to claim 1 strengthens the biodegradable method of azoic dyestuff, and it is characterized in that, the content of described Zero-valent Iron is 6mmolL -1.
CN201310345316.XA 2013-08-08 2013-08-08 Method for strengthening azo dye biodegradation by utilizing zero-valent iron Active CN103387289B (en)

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CN104695238B (en) * 2015-03-11 2016-11-30 西安工程大学 Disperse dyes mineralising in situ, degree of depth water-saving and emission-reducing dyeing post-processing approach and auxiliary agent
CN105347472A (en) * 2015-11-15 2016-02-24 农业部沼气科学研究所 Aerobic biological nitrogen removal process for waste water containing high ammonia-nitrogen
CN107215956A (en) * 2017-06-26 2017-09-29 苏州科技大学 A kind of method of carbon-nanotube enhanced anaerobic sludge Combined Processing azo dye wastewater
CN113087298B (en) * 2021-04-08 2022-08-12 广东工业大学 Device and method for treating organic wastewater based on advanced oxidation and EGSB
CN113480113B (en) * 2021-09-06 2021-11-16 江苏通海染整有限公司 Azo printing and dyeing wastewater treatment process

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