CN113003727A - Bacteria and algae composition for synergistically degrading high-concentration ammonia nitrogen and phosphate and application and method thereof - Google Patents

Bacteria and algae composition for synergistically degrading high-concentration ammonia nitrogen and phosphate and application and method thereof Download PDF

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CN113003727A
CN113003727A CN202110253045.XA CN202110253045A CN113003727A CN 113003727 A CN113003727 A CN 113003727A CN 202110253045 A CN202110253045 A CN 202110253045A CN 113003727 A CN113003727 A CN 113003727A
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bacteria
ammonia nitrogen
microbial inoculum
phosphate
composite microbial
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张千
张楚
赵天涛
艾铄
刘毫
朱宇楠
袁春波
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Chongqing University of Technology
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/30Aerobic and anaerobic processes
    • C02F3/302Nitrification and denitrification treatment
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    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/30Aerobic and anaerobic processes
    • C02F3/301Aerobic and anaerobic treatment in the same reactor
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/32Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • C02F3/322Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
    • C02F3/325Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
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    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/10Inorganic compounds
    • C02F2101/105Phosphorus compounds
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    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/10Inorganic compounds
    • C02F2101/16Nitrogen compounds, e.g. ammonia

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Abstract

The invention discloses a bacteria-algae composition for synergistically degrading high-concentration ammonia nitrogen and phosphate, and an application and a method thereof, wherein the composition consists of a heterotrophic nitrification-aerobic denitrification composite microbial inoculum and chlorella; the compound microbial inoculum is compounded by copper greedy bacteria, alcaligenes faecalis, acinetobacter and ochrobactrum TAC-2; through introducing the chlorella in the composite microbial inoculum, a chlorella symbiotic system is formed, the chlorella generates oxygen through photosynthesis, dissolved oxygen is provided for denitrification of the composite microbial inoculum, aeration energy consumption of the composite microbial inoculum system is reduced, the composite microbial inoculum converts organic matters in wastewater into carbon dioxide through biological action to be required by growth of the chlorella, removal of ammonia nitrogen and total nitrogen by the composite microbial inoculum can be improved, phosphate removal capacity is improved, the problem that the composite microbial inoculum cannot remove phosphorus can be solved, and the composite microbial inoculum can be used for treating high-concentration ammonia nitrogen and phosphate wastewater.

Description

Bacteria and algae composition for synergistically degrading high-concentration ammonia nitrogen and phosphate and application and method thereof
Technical Field
The invention relates to the field of wastewater treatment by using microorganisms, in particular to a bacteria-algae composition for synergistically degrading high-concentration ammonia nitrogen and phosphate, and also relates to an application and a treatment method of the bacteria-algae composition in wastewater treatment.
Background
In recent years, the discharge amount of high-concentration wastewater (ammonia nitrogen concentration is more than 200mg/L) is increasing. The high ammonia nitrogen wastewater is improperly treated and directly discharged into the water body, so that the phenomena of water body eutrophication and the like become serious day by day, and the water body quality is deteriorated. In order to control the ammonia nitrogen pollution of water bodies, new strict pollutant control standards are issued in China, wherein the primary standard of ammonia nitrogen is 5.0 mg/L. The new standard provides new requirements for the effective control of ammonia nitrogen, in particular to the effective control of ammonia nitrogen in high ammonia nitrogen wastewater. Chinese patent publication No. CN109082387A discloses a heterotrophic nitrification-aerobic denitrification complex microbial inoculum capable of removing high ammonia nitrogen at low temperature, which has the advantages of high ammonia nitrogen tolerance, low temperature resistance, high denitrification efficiency, etc., but has the following problems in the practical application of treating high-concentration wastewater (such as breeding wastewater, high ammonia nitrogen, high phosphate): (1) the composite microbial inoculum has high oxygen demand, and the oxygen demand is larger for high-concentration wastewater, so that the energy consumption generated by aeration is high; (2) the composite microbial inoculum only has denitrification capability and no phosphate removal capability, and other modes are needed to remove phosphate in wastewater; (3) the removal efficiency of the compound bacteria on ammonia nitrogen and total nitrogen still needs to be further improved.
Therefore, the improvement of the existing heterotrophic nitrification-aerobic denitrification composite microbial inoculum is urgently needed, the demand of oxygen is reduced, the phosphate removing capability is reduced, and the removing efficiency of ammonia nitrogen and total nitrogen is further improved.
Disclosure of Invention
In view of the above, an object of the present invention is to provide a bacteria-algae composition for synergistically degrading high-concentration ammonia nitrogen and phosphate; the second purpose of the invention is to provide the application of the bacteria and algae composition for synergistically degrading high-concentration ammonia nitrogen and phosphate in removing organic compounds, ammonia nitrogen, total nitrogen and phosphate from wastewater; the invention also aims to provide a method for removing organic compounds, ammonia nitrogen, total nitrogen and phosphate in wastewater by using the bacteria and algae composition for synergistically degrading high-concentration ammonia nitrogen and phosphate.
In order to achieve the purpose, the invention provides the following technical scheme:
1. the bacteria and algae composition for synergistically degrading high-concentration ammonia nitrogen and phosphate comprises a heterotrophic nitrification-aerobic denitrification composite microbial inoculum and chlorella; the composite microbial inoculum is compounded by cuprum greedy strain SWA1(Cupriavidus sp.SWA1) CCTCCNO M2015045, Alcaligenes faecalis (Alcaligenes faecalis) CICC22642, Acinetobacter (Acinetobacter) CICC10695 and Ochrobactrum anthropi TAC-2 (Ochrobactrum sp.TAC-2) CCTCCNO M201802.
The chlorella and the heterotrophic nitrification-aerobic denitrification composite microbial inoculum are utilized to construct a chlorella symbiotic system, so that high-concentration ammonia nitrogen and phosphate in the wastewater can be effectively removed under the condition of a low inoculation amount (10%) of the composite microbial inoculum, the use amounts of the microbial inoculum and the chlorella are reduced, and the operation cost is saved.
Preferably, the volume ratio of the heterotrophic nitrification-aerobic denitrification composite microbial inoculum to the chlorella is 1: 3.
more preferably, the ratio of the number of the cupreous griseus SWA1 (cuprividus sp.SWA1), Alcaligenes faecalis (Alcaligenes faecalis), Acinetobacter (Acinetobacter) and ochrobactrum TAC-2 (Ochrobactrum sp.TAC-2) is 10-20%: 5-20%: 10-30%: 20 to 50 percent.
More preferably, the chlorella is FACH-16 chlorella pyrenoidosa.
2. The bacteria and algae composition for synergistically degrading high-concentration ammonia nitrogen and phosphate is applied to removal of organic compounds, ammonia nitrogen, total nitrogen and phosphate in wastewater.
3. The method for removing organic compounds, ammonia nitrogen, total nitrogen and phosphate in the wastewater by using the bacteria-algae composition for synergistically degrading the high-concentration ammonia nitrogen and phosphate is characterized in that the bacteria-algae composition is inoculated into the wastewater according to the inoculation amount of a composite microbial inoculum of 10 percent and treated under the conditions that the illumination intensity is 2000Lux, the temperature is 28-30 ℃, the air inflow is 0.15-0.20L/min, and the magnetic stirring rotating speed is 100-150 rpm.
The method realizes the efficient removal of high-concentration ammonia nitrogen (500mg/L) and high-concentration phosphate (50mg/L) in the wastewater under the condition of low dissolved oxygen (air inflow of 0.15-0.20L/min), reduces the energy consumption required by aeration, only adds a treatment system of a composite microbial inoculum, the average removal rates of the ammonia nitrogen and the total nitrogen are respectively 62.5% and 47.5%, and the phosphate is not removed but the concentration is increased to a certain extent; in the bacteria-algae symbiotic treatment system, the average removal rate of ammonia nitrogen and total nitrogen is 87.6 percent and 73.4 percent respectively, and the removal rate of phosphate is 45.2 percent, so that the removal of ammonia nitrogen and total nitrogen is remarkably improved by the bacteria-algae symbiotic system, the problem that the composite microbial inoculum cannot remove phosphorus is effectively solved, and the problems of carbon dioxide supply and ammonia nitrogen tolerance of chlorella are solved.
The invention has the beneficial effects that: the invention discloses a bacteria-algae composition for synergistically degrading high-concentration ammonia nitrogen and phosphate, wherein chlorella is introduced into a composite microbial inoculum to form a bacteria-algae symbiotic system, the chlorella generates oxygen through photosynthesis to provide dissolved oxygen for denitrification of the composite microbial inoculum and reduce the aeration energy consumption of the composite microbial inoculum system, the composite microbial inoculum converts organic matters in wastewater into carbon dioxide through biological action to be required by growth of the chlorella, the chlorella also has a denitrification function, the removal of the composite microbial inoculum on ammonia nitrogen and total nitrogen can be improved, meanwhile, the chlorella also has phosphate removal capacity, the problem that the composite microbial inoculum cannot remove phosphorus can be solved, in addition, the composite microbial inoculum has good tolerance characteristics, and the ammonia nitrogen tolerance concentration of the chlorella is improved by adding the composite microbial inoculum.
Drawings
In order to make the object, technical scheme and beneficial effect of the invention more clear, the invention provides the following drawings for explanation:
FIG. 1 shows the removal efficiency of ammonia nitrogen by different bacteria-algae ratios (in the figure, B is a composite microbial inoculum, A is chlorella);
FIG. 2 shows the total nitrogen removal efficiency of different bacteria-algae ratios (in the figure, B is the complex microbial inoculum, A is the chlorella);
FIG. 3 is the average removal rate of ammonia nitrogen by different bacteria-algae ratios;
FIG. 4 shows the average removal rate of total nitrogen by different microalgae ratios.
Detailed Description
The present invention is further described with reference to the following drawings and specific examples so that those skilled in the art can better understand the present invention and can practice the present invention, but the examples are not intended to limit the present invention.
The compound microbial inoculum used by the invention is compounded by cuprum griffithii SWA1 (cupriavidius sp.SWA1), Alcaligenes faecalis (Alcaligenes faecalis), Acinetobacter (Acinetobacter) and ochrobactrum TAC-2 (Ochrobactrum sp.TAC-2), and the patent publication No. CN109082387A refers to Chinese patent. Cupriavidus SWAl (Cupriavidus sp. SWAl) with the preservation number of CCTCC NO: M2015045; acinetobacter (Acinetobacter) with a deposit number of CICC 10695; alcaligenes faecalis (Alcaligenes faecalis) with a deposit number CICC 22642; octopus strain TAC-2 (Ochrobactrum sp.TAC-2) with the preservation number of CCTCC NO: M201802; the composite ratio of the cell numbers of the cupreous greedy bacteria SWA1, the alcaligenes faecalis, the acinetobacter and the ochrobactrum anthropi TAC-2 is 10-20%: 5-20%: 10-30%: 20 to 50 percent.
The chlorella in the invention is FACH-16 Chlorella pyrenoidosa, and is purchased from the institute of aquatic life of Chinese academy of sciences.
Example 1 Codegradation of high concentration Ammonia Nitrogen and phosphate bacteria and algae composition
Mixing the composite microbial inoculum and chlorella according to a volume ratio of 1:3, compounding (wherein the OD value of the compound microbial inoculum is 0.85, the initial OD value of the chlorella is 1.20), and treating the wastewater with the ammonia nitrogen concentration of 500mg/L, the COD concentration of 4000mg/L and the phosphate concentration of 50mg/L under the conditions that the illumination intensity is 2000Lux, the temperature is 28-30 ℃, the air input is 0.15-0.20L/min, the magnetic stirring rotation speed is 100-150rpm according to the inoculation amount of 10 percent of the compound microbial inoculum.
Meanwhile, the same amount of chlorella and composite microbial inoculum is used for treating wastewater, then the concentration of organic Compounds (COD), the concentration of ammonia nitrogen, the concentration of Total Nitrogen (TN) and the concentration of phosphate after treatment are detected, and the COD removal rate, the ammonia nitrogen removal rate, the TN removal rate and the phosphate removal rate are calculated. The results are shown in Table 1.
Figure BDA0002958339560000031
The result shows that under the condition of using the volume ratio of bacteria to algae (1:3), high-concentration COD, ammonia nitrogen (500mg/L), Total Nitrogen (TN) and phosphate in the wastewater are efficiently removed under the condition of low dissolved oxygen (air input amount of 0.15-0.20L/min), wherein the average removal rates of the ammonia nitrogen and the total nitrogen are respectively 87.6% and 73.4%, and the removal rate of the phosphate is 45.2%, which indicates that the bacteria and algae symbiotic system reduces the energy consumption required by aeration, remarkably improves the removal of the ammonia nitrogen and the total nitrogen, effectively solves the problem that the composite microbial inoculum cannot remove phosphorus, and also solves the problems of carbon dioxide supply and ammonia nitrogen tolerance of the chlorella.
Respectively mixing the bacteria and algae in a volume ratio of 1:1, 1:3,1: 5,1: 7, treating the wastewater, and then detecting the high-efficiency removal effect on high-concentration COD, ammonia nitrogen (500mg/L), Total Nitrogen (TN) and phosphate in the wastewater, wherein the result is shown in figures 1-4.
FIG. 1 shows the removal efficiency of ammonia nitrogen by different bacteria and algae ratios; FIG. 2 shows the total nitrogen removal efficiency of different microalgae ratios.
As can be seen from the figures 1 and 2, the addition of chlorella has an obvious promoting effect on the denitrification of HN-AD bacteria, and the removal rate of ammonia nitrogen and total nitrogen is improved by about 20%. The removal rate of pure algae has higher removal rate on ammonia nitrogen and total nitrogen in the wastewater in the first period and the second period, the removal rate of the ammonia nitrogen is up to 90 percent, the removal rate of the total nitrogen is up to 76 percent, but the long-acting property is poor, and the removal rate of the ammonia nitrogen is reduced to 21 percent from the third period; in a certain reaction time, the symbiotic system with the ratio of bacteria to algae of 1:3 has the best effect of removing the total nitrogen, ammonia and nitrogen in the sewage, the ammonia and nitrogen removal rate is maintained at about 85 percent, the total nitrogen removal rate is maintained at about 75 percent, and the system has long-acting property.
FIG. 3 is the average removal rate of ammonia nitrogen by different bacteria-algae ratios; FIG. 4 mean removal of total nitrogen for different algal comparisons.
As can be seen from FIGS. 3 and 4, the removal rates of ammonia nitrogen and total nitrogen show a trend of increasing first and then decreasing along with the decrease of the algae proportion in the system; when the ratio of bacteria to algae is 1:3, the average removal rate of the total nitrogen and ammonia nitrogen of the sewage by the symbiotic system is optimal, the average removal rate of the ammonia nitrogen is 85 percent, and the average removal rate of the total nitrogen is 80 percent.
The above-mentioned embodiments are merely preferred embodiments for fully illustrating the present invention, and the scope of the present invention is not limited thereto. The equivalent substitution or change made by the technical personnel in the technical field on the basis of the invention is all within the protection scope of the invention. The protection scope of the invention is subject to the claims.

Claims (6)

1. The bacteria and algae composition for synergistically degrading high-concentration ammonia nitrogen and phosphate is characterized in that: the composition consists of a heterotrophic nitrification-aerobic denitrification composite microbial inoculum and chlorella; the composite microbial inoculum is compounded by cuprum greedy strain SWA1(Cupriavidus sp.SWA1) CCTCCNO M2015045, Alcaligenes faecalis (Alcaligenes faecalis) CICC22642, Acinetobacter (Acinetobacter) CICC10695 and Ochrobactrum anthropi TAC-2 (Ochrobactrum sp.TAC-2) CCTCCNO M201802.
2. The bacteria and algae composition for synergistically degrading high-concentration ammonia nitrogen and phosphate according to claim 1, wherein the bacteria and algae composition is characterized in that: the volume ratio of the heterotrophic nitrification-aerobic denitrification composite microbial inoculum to the chlorella is 1: 3.
3. the bacteria and algae composition for synergistically degrading high-concentration ammonia nitrogen and phosphate according to claim 1, wherein the bacteria and algae composition is characterized in that: the ratio of the number of the cupreous greedy bacteria SWA1 (cupriavidius sp.SWA1), Alcaligenes faecalis (Alcaligenes faecalis), Acinetobacter (Acinetobacter) and ochrobactrum sp.TAC-2 (Ochrobactrum sp.TAC-2) is 10-20%: 5-20%: 10-30%: 20 to 50 percent.
4. The bacteria and algae composition for synergistically degrading high-concentration ammonia nitrogen and phosphate according to claim 1, wherein the bacteria and algae composition is characterized in that: the chlorella is FACH-16 Chlorella pyrenoidosa.
5. The use of the bacteria and algae composition for synergistically degrading high-concentration ammonia nitrogen and phosphate according to any one of claims 1 to 4 in removing organic compounds, ammonia nitrogen, total nitrogen and phosphate from wastewater.
6. The method for removing organic compounds, ammonia nitrogen, total nitrogen and phosphate in wastewater by using the bacteria and algae composition for synergistically degrading high-concentration ammonia nitrogen and phosphate as claimed in any one of claims 1 to 4 is characterized by comprising the following steps: inoculating the bacteria-algae composition into the wastewater according to the inoculation amount of the composite microbial inoculum of 10 percent, and treating under the conditions that the illumination intensity is 2000Lux, the temperature is 28-30 ℃, the air input is 0.15-0.20L/min, and the magnetic stirring rotating speed is 100-150 rpm.
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CN109082387A (en) * 2018-03-14 2018-12-25 重庆理工大学 It is a kind of can low temperature remove heterotrophic nitrification-aerobic denitrification composite bacteria agent and its application of high ammonia nitrogen
CN113321312A (en) * 2021-06-28 2021-08-31 重庆大学 High concentration of CO2Method for atmosphere domestication of microalgae high ammonia nitrogen resistant biogas slurry
CN113388553A (en) * 2021-07-28 2021-09-14 重庆理工大学 Ammonia nitrogen resistant composite microbial inoculum and application and preparation method thereof
CN115504581A (en) * 2022-10-27 2022-12-23 重庆理工大学 Application of chlorella and heterotrophic nitrification-aerobic denitrification composite microbial inoculum in serving as 3D-RBC biofilm formation inoculum and method

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Publication number Priority date Publication date Assignee Title
CN109082387A (en) * 2018-03-14 2018-12-25 重庆理工大学 It is a kind of can low temperature remove heterotrophic nitrification-aerobic denitrification composite bacteria agent and its application of high ammonia nitrogen
CN109082387B (en) * 2018-03-14 2022-03-18 重庆理工大学 Heterotrophic nitrification-aerobic denitrification composite microbial inoculum capable of removing high ammonia nitrogen at low temperature and application thereof
CN113321312A (en) * 2021-06-28 2021-08-31 重庆大学 High concentration of CO2Method for atmosphere domestication of microalgae high ammonia nitrogen resistant biogas slurry
CN113321312B (en) * 2021-06-28 2022-12-13 重庆大学 High concentration of CO 2 Method for atmosphere domestication of microalgae high ammonia nitrogen resistant biogas slurry
CN113388553A (en) * 2021-07-28 2021-09-14 重庆理工大学 Ammonia nitrogen resistant composite microbial inoculum and application and preparation method thereof
CN115504581A (en) * 2022-10-27 2022-12-23 重庆理工大学 Application of chlorella and heterotrophic nitrification-aerobic denitrification composite microbial inoculum in serving as 3D-RBC biofilm formation inoculum and method

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