CN109082387B - Heterotrophic nitrification-aerobic denitrification composite microbial inoculum capable of removing high ammonia nitrogen at low temperature and application thereof - Google Patents
Heterotrophic nitrification-aerobic denitrification composite microbial inoculum capable of removing high ammonia nitrogen at low temperature and application thereof Download PDFInfo
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Abstract
The invention relates to a heterotrophic nitrification-aerobic denitrification composite microbial inoculum capable of removing high ammonia nitrogen at low temperature and application thereof, wherein the composite microbial inoculum comprises copper greedy bacteria SWA1, alcaligenes faecalis, acinetobacter and ochrobactrum anthropi TAC-2, wherein the proportion of the copper greedy bacteria SWA1, the alcaligenes faecalis, the acinetobacter and the ochrobactrum anthropi TAC-2 is 10-20%: 5-20%: 10-30%: 20 to 50 percent. The composite microbial inoculum has the characteristics of high ammonia nitrogen resistance, low temperature resistance, heavy metal resistance and synchronous nitrification and denitrification function, and can realize the high-efficiency denitrification of high ammonia nitrogen wastewater in an aerobic environment under a low-temperature condition through the synergistic effect of the four microorganisms.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to a heterotrophic nitrification-aerobic denitrification composite microbial inoculum capable of removing high ammonia nitrogen at low temperature and application thereof.
Background
In recent years, the discharge amount of high ammonia nitrogen wastewater (ammonia nitrogen concentration is more than 200 mg/L) is increasing. The high ammonia nitrogen wastewater is improperly treated and directly discharged into the water body, so that the phenomena of water body eutrophication and the like become serious day by day, and the water body quality is deteriorated. In order to control the ammonia nitrogen pollution of water bodies, new strict pollutant control standards are issued in China, wherein the primary standard of ammonia nitrogen is 5.0 mg/L. The new standard provides new requirements for the effective control of ammonia nitrogen, in particular to the effective control of ammonia nitrogen in high ammonia nitrogen wastewater.
Due to the problems of high cost and secondary pollution, the physical and chemical methods for removing ammonia nitrogen in high ammonia nitrogen wastewater (such as a stripping method, an ammonia distillation tower distillation method, a breakpoint chlorination method, a FENTON oxidation method and the like) are only applied to special chemical industries. Biological denitrification is regarded as the most economical and effective water body denitrification method at present due to the advantages of no pollution, economy, safety and the like.
The traditional biological denitrification technology mainly comprises an A/O (anaerobic-aerobic) process, a shortcut nitrification-denitrification process, a nitrification and anaerobic ammonia oxidation process and the like. The biological denitrification technologies all utilize the characteristics of autotrophic nitrifying bacteria to achieve the aim of removing ammonia nitrogen. However, a large amount of free ammonia contained in the high-concentration ammonia-nitrogen wastewater has a strong inhibiting effect on autotrophic nitrifying bacteria, so that the ammonia oxidation process of the autotrophic nitrifying bacteria is inhibited, the ammonia-nitrogen treatment efficiency is influenced, and the treatment effect of the biological methods is poor when the high-ammonia-nitrogen wastewater is treated; in addition, the optimum growth temperature range of the autotrophic nitrifying bacteria is 25-35 ℃, when the temperature is lower than 10 ℃, the autotrophic nitrifying bacteria stop growing, the nitrification reaction is seriously influenced, and the problems of poor wastewater treatment quality, low treatment efficiency, high treatment cost and the like are caused.
Disclosure of Invention
The invention aims to provide a heterotrophic nitrification-aerobic denitrification composite microbial inoculum capable of removing high ammonia nitrogen at low temperature aiming at the defects of the prior art, the composite microbial inoculum has the characteristics of high ammonia nitrogen resistance, low temperature resistance, heavy metal resistance and synchronous nitrification-denitrification function, and the high-ammonia nitrogen wastewater can be efficiently denitrified in an aerobic environment under the low-temperature condition through the synergistic action of four microorganisms.
The Total Nitrogen (TN) in the invention refers to ammonia Nitrogen (NH)4 +-N\ NH3-N), nitrate Nitrogen (NO)3 -) Nitrite Nitrogen (NO)2 -) The sum of (a) and (b).
The technical scheme of the invention is as follows: heterotrophic nitrification-aerobic denitrification composite microbial inoculum capable of removing high ammonia nitrogen at low temperature, wherein the composite microbial inoculum is prepared from cuppridinium bulimia SWA1 (aCupriavidussp, SWA 1), Alcaligenes faecalis (A. faecalis: (A. faecalis)Alcaligenes faecalis) Acinetobacter (A), (B), (C)Acinetobacter) And Ochrobactrum TAC-2 (Ochrobacterumsp, TAC-2).
The compound proportion of the cuppridinium greedy SWA1, the Alcaligenes faecalis, the Acinetobacter and the Xanthium sibiricum TAC-2 is 10-20%: 5-20%: 10-30%: 20 to 50 percent.
The 16S rDNA sequence of the ochrobactrum anthropi TAC-2 is shown as SEQ ID NO: 1.
The preservation number of the cupreous greedy strain SWA1 is CCTCC NO: m2015045, the preservation number of the Alcaligenes faecalis is CICC 22642, the preservation number of the Acinetobacter TAC-1 is CICC 10695, and the preservation number of the ochrobactrum TAC-2 is CCTCC NO: M2018028.
The preparation method of the composite microbial inoculum comprises the following steps,
1) domestication of strains
Performing low-temperature and high ammonia nitrogen tolerance domestication on the strains by adopting an ammonia nitrogen gradient domestication method at the temperature of 5 ℃;
2) expanded culture of strains
Using carbon-containing compounds as carbon sources, and respectively carrying out expanded culture on the strains domesticated in the step 1) in a heterotrophic nitrification culture medium;
3) preparation of complex microbial inoculum
Mixing the bacterial liquid obtained in the step 2) according to the compound proportion of each strain to obtain a seed liquid, and carrying out enrichment culture on the seed liquid until the OD600 =0.7 +/-0.1 to obtain the compound microbial inoculum.
The method for expanding culture in the step 2) comprises the following steps:
cupriavium japonicum SWA1
Taking 10-20 mL of bacterial liquid to be added into a heterotrophic nitrification culture medium, sealing, and carrying out shake culture for 4d at the temperature of 30 ℃ and at the speed of 150 r/min;
alcaligenes faecalis
Taking 10-20 mL of bacterial liquid to be added into a heterotrophic nitrification culture medium, sealing, and carrying out shake culture for 4d at the temperature of 30 ℃ and at the speed of 150 r/min;
acinetobacter
Taking 10-20 mL of bacterial liquid to be added into a heterotrophic nitrification culture medium, sealing, and carrying out shake culture for 4d at the temperature of 30 ℃ and at the speed of 150 r/min;
ochrobactrum TAC-2
And (3) taking 10-20 mL of bacterial liquid to the heterotrophic nitrification culture medium, sealing, and carrying out shake culture for 4d at the temperature of 30 ℃ and at the speed of 150 r/min.
The composite microbial inoculum is applied to denitrification in wastewater.
The cupreous greedy bacterium of the present inventionCupriavidussp, SWA1 belonging to genus Cupriavidus, with colony diameter of about 1 mm, translucency, protrusion, regular edge, glossy surface, and white and transparent color; gram staining was negative. The optimal growth temperature is 25-40 ℃, and the pH is 6.5-7.5.
Alcaligenes faecalisAlcaligenes faecalis(CICC 22642) belonging to the genus Alcaligenes faecalis. The diameter is 0.5-1.0 mm, the length is 0.5-2.6 mm, and the single thallus arrangement is usually adopted, so no spores are produced. Gram-negative, the optimum growth temperature is 20-37 ℃, the optimum pH value is about 7.
AcinetobacterAcinetobacte（CICC 10695）The bacillus subtilis belongs to the acinetobacter, is gram-negative, has the thallus size of 1.5-2.5 UM, and is enveloped, spore-free, flagellated and immobile. Obligate aerobe, optimal growth temperature 28 ℃.
Ochrobactrum anthropiOchrobacterumsp, TAC-2, belonging to the genus Ochrobactrum. The diameter of the bacterial colony is about 1 mm, and the bacterial colony is semitransparent, protruding, neat in edge, glossy in surface, white and transparent in color; gram staining was negative. The optimal growth temperature is 25-40 ℃, and the pH is 6.5-7.5.
The cupronickel bacteria in the composite microbial inoculumCupriavidussp. SWA1 (CCTCC NO: M2015045) is disclosed in the patent with the publication number of "CN 104830725A".
The alcaligenes faecalis in the composite microbial inoculum is purchased from China center for culture Collection of Industrial microorganisms (number CICC 22642).
Acinetobacter in the complex microbial inoculum is purchased from China center for Industrial culture Collection of microorganisms (number CICC 10695).
The collection number of the ochrobactrum glaucoides TAC-2 in the composite microbial inoculum is 'CCTCC NO: M2018028' (the ochrobactrum glaucoides TAC is preserved in China center for type culture Collection in 1 month and 12 days in 2018, and the address is university of Wuhan Han, China), and the classification is named asOchrobacterumsp. TAC-2, the 16S rDNA sequence of which is as described in SEQ ID NO 1.
The heterotrophic nitrification-aerobic denitrification composite microbial inoculum capable of removing high ammonia nitrogen at low temperature provided by the invention has the following beneficial effects:
(1) in the composite microbial inoculum provided by the invention, the cold resistance of each strain is activated by low-temperature domestication and screening, so that each strain can still grow rapidly under a low-temperature condition (5 ℃) and keep high activity for a long time; the four strains of the composite microbial inoculum act synergistically to realize high-efficiency denitrification under the low-temperature condition;
(2) the composite microbial inoculum provided by the invention has stronger tolerance capability to high ammonia nitrogen wastewater under a low temperature condition (5 ℃), can realize a process of synchronous nitrification and denitrification in an aerobic environment under the conditions of high ammonia nitrogen and high salinity, effectively removes total nitrogen in the high ammonia nitrogen wastewater and the high salinity wastewater, is particularly suitable for removing ammonia nitrogen in the high ammonia nitrogen wastewater, and realizes the purpose of efficiently removing high-concentration ammonia nitrogen (the concentration is more than 400 mg/L) at a low temperature;
(3) the composite microbial inoculum provided by the invention is convenient to use and small in dosage, can be directly put into a water body to form dominant bacteria to efficiently remove total nitrogen and ammonia nitrogen in the water body, and has the advantages of high treatment efficiency and low treatment cost.
The composite microbial inoculum can effectively solve the problems of poor treatment effect, low treatment efficiency, high treatment cost and the like of ammonia nitrogen wastewater in the traditional biological denitrification treatment under the environment of low temperature (5 ℃) and high ammonia nitrogen.
The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.
Drawings
FIG. 1 is a graph showing the removal of ammonia nitrogen and total nitrogen by a complex microbial inoculum at 5 ℃ and an ammonia nitrogen concentration of 400 mg/L;
FIG. 2 is a graph showing the removal of high ammonia nitrogen by a complex microbial inoculum under different temperature conditions;
FIG. 3 is a graph showing the removal of ammonia nitrogen from wastewater in a pig farm by using the complex microbial inoculum at 5 ℃.
Detailed Description
1. Experimental Material
Heterotrophic nitrification culture medium: (NH)4)2SO4 2 g/L，Na3C6H5O710.64 g/L, 50 mL/L Vickers salt solution, wherein Vickers salt solution (g/L): k2HPO4 5.0，MgSO4 7H2O 2.5，NaCl 2.5，FeSO4 7H2O 0.05，MnSO44H2And O is 0.05. The pH of the heterotrophic nitrification medium was adjusted to 7.0.
The rest reagents are commercial analytical pure products.
2. Detection method
And detecting the OD value of the bacterial liquid by using a UV2000 spectrophotometer, wherein the wavelength is 600 nm. Reference is made to "Water and wastewater monitoring and analysis method" (fourth edition, published by Chinese environmental science, 2002) for methods for monitoring and analyzing various pollutants.
Example 1:
step 1) domestication of strains
Cupridinium avidumCupriavidussp. SWA1 low temperature, high ammonia nitrogen tolerance acclimation: inoculating 2mL of the bacterial solution into a 250 mL triangular flask containing 100 mL of sterilized heterotrophic nitrification culture medium, shaking thoroughly, and performing fermentation at 5 deg.C for 150 r min-1Culturing under the condition for 2 d. Continuously subculturing under the same conditions, and subculturing for 5 times, wherein the ammonia nitrogen concentrations are 200 mg/L, 300 mg/L, 400 mg/L, 500 mg/L and 600 mg/L respectively.
Alcaligenes faecalisAlcaligenes faecalis(CICC 22642) low-temperature and high ammonia nitrogen tolerance domestication: inoculating 2mL of the bacterial solution into a 250 mL triangular flask containing 100 mL of sterilized heterotrophic nitrification culture medium, shaking thoroughly, and performing fermentation at 5 deg.C for 150 r min-1Culturing under the condition for 2 d. Continuously subculturing under the same conditions, and continuously increasing the ammonia nitrogen concentration of each subculturing to realize domestication of the strain.
AcinetobacterAcinetobacter(CICC 10695) low-temperature and high ammonia nitrogen tolerance domestication: inoculating 2mL of the bacterial solution into a 250 mL triangular flask containing 100 mL of sterilized heterotrophic nitrification culture medium, shaking thoroughly, and performing fermentation at 5 deg.C for 150 r min-1Culturing under the condition for 2 d. Continuously subculturing under the same conditions without subculturingThe concentration of ammonia nitrogen is increased continuously to realize the domestication of the strain.
Ochrobactrum anthropiOchrobacterumAcclimating sp, TAC-2 at low temperature and high ammonia nitrogen tolerance: inoculating 2mL of the bacterial solution into a 250 mL triangular flask containing 100 mL of sterilized heterotrophic nitrification culture medium, shaking thoroughly, and performing fermentation at 5 deg.C for 150 r min-1Culturing under the condition for 2 d. Continuously subculturing under the same conditions, and continuously increasing the ammonia nitrogen concentration of each subculturing to realize domestication of the strain.
Step 2) expanded culture of strains
Cupridinium avidumCupriavidusAmplification culture method of sp, SWA1
Inoculating 10-20 mL of the bacteria solution subjected to low-temperature and high-ammonia nitrogen acclimatization into a 2000 mL triangular flask containing 1000 mL of sterilized heterotrophic nitrification culture medium, sufficiently shaking up, and carrying out fermentation at 30 ℃ for 150 r min-1Culturing under the condition of 4 d.
Alcaligenes faecalisAlcaligenes faecalis(CICC 22642) amplification culture method
Inoculating 10-20 mL of the bacteria solution subjected to low-temperature and high-ammonia nitrogen acclimatization into a 2000 mL triangular flask containing 1000 mL of sterilized heterotrophic nitrification culture medium, sufficiently shaking up, and carrying out fermentation at 30 ℃ for 150 r min-1Culturing under the condition of 4 d.
AcinetobacterAcinetobacte（CICC 10695）The method of the expanded culture of (3)
Inoculating 10-20 mL of the bacteria solution subjected to low-temperature and high-ammonia nitrogen acclimatization into a 2000 mL triangular flask containing 1000 mL of sterilized heterotrophic nitrification culture medium, sufficiently shaking up, and carrying out fermentation at 30 ℃ for 150 r min-1Culturing under the condition of 4 d.
Ochrobactrum anthropiOchrobacterumAmplification culture method of sp, TAC-2
Inoculating 10-20 mL of the bacteria liquid subjected to low-temperature and high-ammonia nitrogen acclimatization into a 2000 mL triangular flask filled with 1000 mL of sterilized heterotrophic nitrification culture medium, fully shaking up, and culturing for 4 days at 30 ℃ under the condition of 150 r.min < -1 >.
3) Preparation of complex microbial inoculum
Taking the cupprid bacteria obtained in the step 2)Cupriavidus1-2 ml of sp, SWA1 bacterial liquid and alcaligenes faecalisAlcaligenes faecalis（CICC 22642) bacterial liquid 1-4 ml, acinetobacterAcinetobacter(CICC 10695) bacterial liquid 1-3 ml, ochrobactrumOchrobacterumAnd mixing 2-5 ml of sp, TAC-2 bacterial liquid to obtain seed liquid.
Inoculating 20 mL of the seed solution into a 2000 mL triangular flask containing 1000 mL of sterilized heterotrophic nitrification medium, shaking thoroughly, and culturing at 30 deg.C for 150 r min-1Culturing under the condition of 4 d. When OD is reached600After the value is increased to 0.7, the stationary phase is reached, and the composite microbial inoculum is obtained.
Example 2: experiment for removing high ammonia nitrogen at 5 ℃ by using composite microbial inoculum
Preparing heterotrophic nitrification culture medium with ammonia nitrogen concentration of 400 mg/L, adding 2mL of compound microbial inoculum liquid (OD 600 is 1-2) into 100 mL heterotrophic nitrification culture medium in 250 mL conical flask by using micropipettor, sealing with sealing film, placing in shaking table at 5 deg.C and 150 r.min-1Culturing under the condition, determining the growth condition of the bacteria at intervals of 24 h by measuring the OD600 value of the bacteria liquid, simultaneously determining the content of ammonia nitrogen and total nitrogen in the culture medium, and determining the removal effect of the ammonia nitrogen and the total nitrogen, wherein the removal rate of the ammonia nitrogen of the composite bacteria agent reaches 100% and the removal rate of the total nitrogen reaches 79.05% under the condition of 5 ℃ as can be seen from figure 1.
Example 3: experiment for removing high ammonia nitrogen by composite microbial inoculum at different temperatures
Preparing heterotrophic nitrification culture medium with ammonia nitrogen concentration of 400 mg/L, adding 2mL of compound microbial inoculum liquid (OD 600 is 1-2) into 100 mL of heterotrophic nitrification culture medium in 250 mL of conical flask by using micropipettor, sealing with sealing membrane, and respectively placing different conical flasks into different shaking tables (with temperature set at 5 deg.C, 10 deg.C, 15 deg.C, 20 deg.C, 25 deg.C, 35 deg.C, and rotation speed set at 150 r.min-1) And (3) performing medium culture, then determining the OD600 value of the bacterial liquid every 24 h to determine the growth condition of the bacteria, and simultaneously determining the content of ammonia nitrogen in the culture medium to determine the removal effect of the ammonia nitrogen. As can be seen from FIG. 2, the composite microbial inoculum can realize the high-efficiency removal of ammonia nitrogen within the range of 5-35 ℃.
Example 4: experiment for treating piggery wastewater by using composite microbial inoculum at 5 DEG C
100 mL of pig farm wastewater (obtained from Chongqing Wood elk pig farm, Chongqing CityBanan ginger, town, Cai, Muyun) in a 250 mL conical flask, adding 2mL of complex microbial inoculum liquid (OD 600 is 1-2) into the flask by using a micropipettor, sealing with sealing film, placing in a shaking table, setting at 5 deg.C and 150 r.min-1Culturing under the condition, measuring the content of ammonia nitrogen in the wastewater every 24 hours, and determining the removal effect of the ammonia nitrogen, wherein as can be seen from figure 3, the composite microbial inoculum can efficiently remove the ammonia nitrogen in the wastewater of a pig farm at the temperature of 5 ℃, and the removal rate can reach 81.76%.
The applicant found in experiments that cupreous polyprioides can be obtained under other conditionsCupriavidus10-20% of sp, SWA1, and alcaligenes faecalisAlcaligenes faecalis(CICC 22642) in a proportion of 5-20%, acinetobacterAcinetobacter(CICC 10695) in a proportion of 10-30 percent, and ochrobactrum anthropiOchrobacterumThe sp and TAC-2 proportion is 20-50%, and the experimental effect of the invention can be achieved. When the volume ratio of the composite microbial inoculum to the wastewater is 1-10%, the experimental effect of the invention can be achieved, time factors and economic factors are comprehensively considered, and when the volume of the composite microbial inoculum is 2.0-4.0% of the volume of the wastewater, the effect of removing high ammonia nitrogen is optimal.
Sequence listing
<110> Chongqing university of science and technology
<120> heterotrophic nitrification-aerobic denitrification composite microbial inoculum capable of removing high ammonia nitrogen at low temperature and application thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 425
<212> DNA
<213> Ochrobactrum sp. TAC-2 16S rDNA sequence)
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gtggggaata ttgcacaatg ggggaaaccc tgatgcagca acgccgcgtg agtgatgacg 60
gtcttcggat tgtaaagctc tgtctttggg gacgataatg acggtaccca aggaggaagc 120
cacggctaac tacgtgccag cagccgcggt aatacgtagg tggcgagcgt tgtccggatt 180
tactgggcgt aaagggagcg taggcggatt cttaagtggg atgtgaaata cctgggctta 240
acctgggtgc tgcattccaa actgggaatc tagagtgcag gaggggagag tggaattcct 300
agtgtagcgg tgaaatgcgt agagattagg aagaacacca gtggcgaagg cgactctctg 360
gactgtaact gacgctgagg ctcgaaagcg tggggagcaa acaggattag aaacccctgt 420
agtcc 425
Claims (5)
1. A heterotrophic nitrification-aerobic denitrification composite microbial inoculum capable of removing high ammonia nitrogen at low temperature is characterized in that the composite microbial inoculum is prepared from cupronickel bacteria (copper-greedy bacteria)Cupriavidussp) SWA1, Alcaligenes faecalis ((S)Alcaligenes faecalis) Acinetobacter (A), (B), (C)Acinetobacter) And Ochrobactrum anthropi: (Ochrobacterumsp) TAC-2;
wherein the preservation number of the cupreous polyprioides SWA1 is CCTCC NO: m2015045, wherein the preservation number of the alcaligenes faecalis is CICC 22642, the preservation number of the acinetobacter is CICC 10695, and the preservation number of the ochrobactrum anthropi TAC-2 is CCTCC NO: M2018028.
2. The complex microbial inoculant according to claim 1, wherein the complex proportion of cuppridinium bulimia SWA1, Alcaligenes faecalis, Acinetobacter and Xanthium vulgare TAC-2 is 10-20%: 5-20%: 10-30%: 20 to 50 percent.
3. A preparation method of a complex microbial inoculum is characterized by comprising the following steps,
1) domestication of strains
Under the condition of 5 ℃, an ammonia nitrogen gradient domestication method is adopted, and the preservation number is CCTCC NO: m2015045 cupronickel bacterium SWA1, the number of Alcaligenes faecalis with the collection number of CICC 22642, the number of Acinetobacter with the collection number of CICC 10695 and the number of CCTCC NO of M2018028, and the Ochrobactrum anthropi TAC-2 are respectively subjected to low-temperature and high-ammonia nitrogen tolerance domestication;
2) expanded culture of strains
Using carbon-containing compounds as carbon sources, and respectively carrying out expanded culture on the strains domesticated in the step 1) in a heterotrophic nitrification culture medium;
3) preparation of complex microbial inoculum
Taking the bacterial liquid obtained in the step 2), mixing the bacterial liquid according to the proportion of claim 2 to obtain seed liquid, and carrying out enrichment culture on the seed liquid until OD600 =0.7 +/-0.1 to obtain the composite microbial inoculum.
4. The method according to claim 3, wherein the step 2) comprises the step of performing the scale-up culture by: and (3) taking 10-20 mL of each bacterial liquid, respectively adding the bacterial liquids into the heterotrophic nitrification culture medium, sealing, and carrying out shaking independent culture for 4d at the temperature of 30 ℃ and at the speed of 150 r/min.
5. Use of the complex microbial inoculum according to any one of claims 1-2 in denitrification of wastewater.
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| CN110982732B (en) * | 2019-11-13 | 2022-12-30 | 重庆理工大学 | Salt-tolerant and high-ammonia-nitrogen-resistant heterotrophic nitrification-aerobic denitrification composite microbial agent and preparation and application thereof |
| CN110804568B (en) * | 2019-11-14 | 2021-03-02 | 重庆理工大学 | Complex microbial inoculant and application thereof |
| CN111154680B (en) * | 2020-01-15 | 2022-07-15 | 北京中源创能工程技术有限公司 | Compound microbial agent for treating organic garbage and application thereof |
| CN111233148A (en) * | 2020-01-19 | 2020-06-05 | 重庆理工大学 | Efficient biomembrane synchronous nitrification and denitrification low-carbon sewage denitrification process |
| CN111233166A (en) * | 2020-01-19 | 2020-06-05 | 重庆理工大学 | Method for biofilm formation of biofilm reactor by using microbial inoculum |
| CN111422976A (en) * | 2020-03-09 | 2020-07-17 | 重庆理工大学 | Process for treating high ammonia nitrogen wastewater by using moving bed biofilm reactor |
| CN111924961A (en) * | 2020-06-30 | 2020-11-13 | 重庆理工大学 | Novel combined process for treating chemical wastewater |
| CN112175863B (en) * | 2020-09-15 | 2022-05-13 | 重庆理工大学 | Preparation method and application of complex microbial inoculant for removing nitrogen-containing pollutants in high-salinity wastewater |
| CN113003727A (en) * | 2021-03-02 | 2021-06-22 | 重庆理工大学 | Bacteria and algae composition for synergistically degrading high-concentration ammonia nitrogen and phosphate and application and method thereof |
| CN113388553B (en) * | 2021-07-28 | 2023-05-16 | 重庆理工大学 | Ammonia nitrogen-resistant composite microbial inoculant, application and preparation method thereof |
| CN115286118B (en) * | 2022-02-16 | 2023-10-20 | 沈阳大学 | Method for treating sewage by using low-temperature-resistant denitrification and dephosphorization composite microbial agent |
| CN116769683B (en) * | 2023-08-22 | 2023-10-20 | 中国科学院烟台海岸带研究所 | Pallidum J-22 capable of simultaneously realizing denitrification and heavy metal removal of water body |
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