CN107699513B - Black and odorous water body degrading bacterium and application thereof - Google Patents
Black and odorous water body degrading bacterium and application thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/11—Bacillus megaterium
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
Abstract
The invention provides a special degrading strain for black and odorous water and application thereof in treatment of black and odorous water, wherein the strain is bacillus megaterium, the preservation number is CGMCC No.13543, and the strain reduces COD of the black and odorous water, degrades ammonia nitrogen in the black and odorous water and/or removes phosphate. The special degrading strain for the black and odorous water body has strong specificity on pollutants in the black and odorous water body, can efficiently utilize organic matters and ammonia nitrogen in the black and odorous water body, can reduce COD (chemical oxygen demand) of the black and odorous water body by more than 90 percent, has good phosphorus removal capacity, and can greatly improve the water quality purification efficiency of the black and odorous water body.
Description
Technical Field
The invention belongs to the field of microorganisms, and particularly relates to a special degrading bacterium for black and odorous water and application thereof.
Background
Black and odorous water is a generic term for water that exhibits unpleasant colors (black and blackish) and/or emits unpleasant odors (smells or malodors). In recent years, the condition of excessive sewage collection exists in most of urban riverways, closed and semi-closed pond water bodies in China. Excessive discharge directly leads to eutrophication of water body, imbalance of oxygen supply and consumption of the water body, and rising of the concentrations of ammonia nitrogen, hydrogen sulfide, iron, manganese sulfide and the like, thereby causing continuous deterioration of water quality and accompanying black and odorous phenomena. Organic matter pollution of water is one of important influencing factors causing black and smelly water. Organic substances such as saccharides, amino acids, proteins, fats and esters mainly exist in a dissolved state and a suspended state, and the substances are decomposed into small molecular substances under the action of microorganisms, so that a large amount of dissolved oxygen is consumed in the process. Inorganic pollutants mainly playing a role in blackening are contained in the black and odorous water body, wherein the main blackening components are FeS and MnS which are easy to oxidize. Chemical Oxygen Demand (COD) reflects the degree of contamination of water by reducing substances, including organics, nitrites, ferrous salts, sulfides, etc., and thus is a comprehensive indicator of the relative content of reducing substances.
The black and odorous water treatment technology mainly comprises the technologies of artificial aeration, ecological engineering restoration, biological growth promotion, microbial purification, combined biological and plant purification and the like. The method for metabolizing high-concentration pollutants by using special microorganisms is a main method and development trend for treating black and odorous water at home and abroad at present. The efficient special degrading bacteria can increase dissolved oxygen by using organic matters, hydrogen sulfide, ammonia and the like as hydrogen donor to synthesize self-constituent substances in severely polluted water bodies, thereby playing a role in purifying sewage. At present, the number of special microorganisms in the black and odorous water body is limited, the degradation effect is uneven, and related process technologies are in need of development. In related products, part of high-efficiency special microbial inoculants need to be imported, so that the cost is increased, and the rapid development of a technical service market is influenced. Therefore, the development of microbial strains and microbial agents special for black and odorous water is urgently needed to serve relevant municipal departments and technical markets by combining the specific requirements of water treatment in China.
Disclosure of Invention
In order to obtain a novel microbial inoculum treatment system which operates efficiently, the inventor does a great deal of work, and separates and screens out efficient water pollutant degrading strains from sludge according to natural distribution and metabolic rules of water microorganisms aiming at typical characteristics of black and odorous water in China.
Therefore, the invention provides a special degradation strain for black and odorous water, which is bacillus megaterium named AW0F5 and is preserved in China general microbiological culture Collection center of China Committee for culture Collection of microorganisms No. 3 of North Chenlu No.1 Hospital in the south-facing area of Beijing in 2017 for 1 month and 10 days, and the preservation number is CGMCC No. 13543. The 16S rDNA sequence of AW0F5 is shown in SEQ ID NO. 1.
The screening steps of the strain are as follows: sludge taken from sewage treatment plant of Jizhuanzi in Tianjin CityImmediately putting the collected sample in a sterilization container and bringing back the sample; taking 5g of sludge in an LB liquid culture medium under an aseptic condition, and carrying out shake amplification culture for 24 hours; 5mL of culture solution is taken and transferred into LB liquid culture medium to be cultured for 24h (repeated for 3 times); taking 1mL of LB culture solution for the last time to dilute 103-108Coating the diluent on a Gao's 1 solid culture medium; culturing at constant temperature of 30 ℃, and repeatedly streaking and separating after bacterial colonies appear until pure single bacterial colonies appear.
The invention also provides a method for amplifying the degradation bacterial strain special for the black and odorous water body, which uses an LB liquid culture medium; the culture temperature is 5-55 deg.C, preferably 30-40 deg.C; the pH is 6.8-8.0, preferably 7.2-7.8.
The invention also provides another method for amplifying the degradation strain special for the black and odorous water body, which uses a Gao's No.1 liquid culture medium; the culture temperature is 15-45 ℃, preferably 25-40 ℃; the pH value is 6.8-7.5.
The invention provides application of a special degrading strain for black and odorous water in treatment of black and odorous water.
In a specific embodiment of the invention, the degradation strain dedicated to black and odorous water body reduces COD of black and odorous water body.
In another specific embodiment of the invention, the degradation strain specially used for the black and odorous water body degrades ammonia nitrogen and/or removes phosphate in the black and odorous water body.
In another specific embodiment of the invention, the black and odorous water body comes from a river channel, a pond and a ditch.
In another embodiment of the present invention, the pH of the black and odorous water body is 6.5 to 8.5, preferably 7.5 to 8.2; the temperature of the black and odorous water body is 10-40 ℃, and the preferable temperature is 23-30 ℃; the state of the black and odorous water body is standing, shaking or aeration, preferably shaking or aeration.
The invention also provides a microbial agent for treating the black and odorous water body, which comprises the degradation bacteria special for the black and odorous water body.
The special degrading strain for the black and odorous water body has strong specificity on pollutants in the black and odorous water body, can efficiently utilize organic matters and ammonia nitrogen in the black and odorous water body, can reduce COD (chemical oxygen demand) of the black and odorous water body by more than 90 percent, has good phosphorus removal capacity, and can greatly improve the water quality purification efficiency of the black and odorous water body.
Detailed Description
Example 1: isolation, screening and identification of strains
Taking sludge from a sewage treatment plant of Jizhuang of Tianjin city, immediately placing a sample in a sterilization container after collection and bringing back the sample; taking 5g of sludge in an LB liquid culture medium under an aseptic condition, and carrying out shake amplification culture for 24 hours; 5mL of culture solution is taken and transferred into LB liquid culture medium to be cultured for 24h (repeated for 3 times); taking 1mL of LB culture solution for the last time to dilute 103-108And (3) coating the diluent on an LB solid culture medium, culturing at the constant temperature of 37 ℃, and repeatedly streaking and separating after bacterial colonies appear until pure single bacterial colonies appear.
Another separation and screening mode: taking sludge from a sewage treatment plant of Jizhuang of Tianjin city, immediately placing a sample in a sterilization container after collection and bringing back the sample; taking 5g of sludge in an LB liquid culture medium under an aseptic condition, and carrying out shake amplification culture for 24 hours; 5mL of culture solution is taken and transferred into LB liquid culture medium to be cultured for 24h (repeated for 3 times); taking 1mL of LB culture solution for the last time to dilute 103-108Coating the diluent on a Gao's 1 solid culture medium; culturing at constant temperature of 30 ℃, and repeatedly streaking and separating after bacterial colonies appear until pure single bacterial colonies appear.
10 pure single colonies were picked and transferred to LB slant medium for storage at 4 ℃.
10 strains were inoculated into an ammonia nitrogen-enriched LB plate medium (glucose 5g, (NH) respectively4)2SO42g，NaCl2g，FeSO4·7H2O 0.4g，K2HPO41g，MgSO4·7H2O0.5 g, pH 7.2), and left to stand at 37 ℃ for 3 days, wherein AW0F5 survives.
10 strains were inoculated on phosphorus-enriched LB plate medium (10 g of glucose per 1000mL of water, (NH4)2SO40.5g，MgSO4·H2O 0.3g，NaCl0.3g，KCl0.3g， FeSO4·7H2O 0.03g，MnSO4·H2O0.03g，K2HPO40.5g, pH 7.2), static culture at 37 ℃ for 5 days, in which AW0F5 survived and appearedAnd (4) dissolving a phosphorus ring.
The AW0F5 strain was transferred to LB slant medium and stored at 4 ℃.
The strain AW0F5 shows positive gram stain, and when LB culture medium is used, the conditions of the strain survival and growth reproduction are that the temperature is 5-55 ℃, and the pH value is 6.5-8.0. When the bacterial colony is cultured on a flat plate, the bacterial colony is circular, the surface is smooth and slightly convex, the edge is regular, the color is opaque milky white, and a single thallus is rod-shaped. The length of the strain is 3.0-4.0 μm after 24 hours of LB culture medium culture. 16S bacteria conserved sequence PCR amplification is carried out by adopting 16S universal primers 27F and 1492R, and the obtained rDNA sequence is subjected to BLAST homology comparison, so that the obtained rDNA sequence has 97 percent of homology with at least 15 strains of Bacillus megaterium (sequenced by Beijing Liuhua Dagenescience and technology Co., Ltd.), and can be preliminarily identified as the Bacillus megaterium. The 16S rDNA sequence of AW0F5 is shown in SEQ ID NO. 1.
SEQIDNO.1：
Example 2: degradation of ammonia nitrogen
Preparing an ammonia nitrogen liquid culture medium: glucose 1g, (NH)4)2SO40.4g，NaCl2g，FeSO4·7H2O 0.4g，K2HPO41g，MgSO4·7H2O0.5 g, 1000mL deionized water, pH 7.2, 121 ℃ sterilization for 20 min.
The strain AW0F5 is inoculated to LB liquid culture medium from a slant culture medium, and cultured for 24 hours at the temperature of 30 ℃ at 110r/min to prepare seed liquid. The thalli is collected by centrifugation at 2000r/min for 7 min. Preparing the thalli into bacterial suspension by using 3mL of sterile water, inoculating 0.5mL of the bacterial suspension into an ammonia nitrogen liquid culture medium, setting a blank group without the thalli, and culturing at the temperature of 23 ℃ under the condition of 110 r/min. And measuring the ammonia nitrogen concentration every 24 hours, and calculating the ammonia nitrogen degradation rate.
In the ammonia nitrogen liquid culture medium (NH)4)2SO4(also referred to as ammonia nitrogen in the application) is a unique nitrogen source, the initial concentration of the ammonia nitrogen is 85.5mg/L, and the degradation rate of the ammonia nitrogen of the substrate reaches 55.2%, 63.9% and 85.6% after 1 day, 7 days and 10 days.
The ammonia nitrogen degradation rate calculation formula is as follows:
wherein X is the ammonia nitrogen degradation rate; m1 is the experimental ammonia nitrogen concentration (mg/L); m2 is blank ammonia nitrogen concentration (mg/L); m is the initial ammonia nitrogen concentration (mg/L).
Example 3: phosphorus removal
Preparing an ammonia nitrogen liquid culture medium: CH (CH)3COONa 1g，MgSO4·7H2O 0.082g，FeSO4·7H2O 0.0037g，CaCl20.06g，(NH4)2SO40.2g, beef extract 0.22g, K2HPO40.057g, pH 7.2, sterilizing at 121 ℃ for 20 min.
Inoculating the strain AW0F5 from slant culture medium to LB liquid culture medium, and culturing at 30 deg.C for 24 hr at 110r/min to obtain seed liquid. The thalli is collected by centrifugation at 2000r/min for 7 min. Preparing the thalli into bacterial suspension by using 3mL of sterile water, inoculating 0.5mL of the bacterial suspension into an ammonia nitrogen liquid culture medium, setting a blank group without the thalli, and culturing at the temperature of 23 ℃ under the condition of 110 r/min. Measuring the phosphate radical concentration every 24 hours, and calculating the phosphorus removal rate.
The initial phosphate concentration was determined to be 23.75mg/L with a phosphorus removal rate of 57.9% 7 days after inoculation.
And the phosphorus removal rate calculation formula is as follows:
wherein Y is the phosphorus removal rate; n1 is the concentration of phosphate in the experimental group (mg/L); n2 is blank phosphate concentration (mg/L); n is the initial phosphate concentration (mg/L).
Example 4: degradation of black and odorous water raw water
The black and odorous water body used in the embodiment is a water body of a certain pool in Qingguangzhou area in Tianjin city; through determination, the COD of the raw water of the black and odorous water bodyCrIs 240 mg/L; the ammonia nitrogen content is 50 mg/L; the phosphorus content was 13 mg/L.
Inoculating the strain AW0F5 from slant culture medium to LB liquid culture medium, and culturing at 30 deg.C for 24 hr at 110r/min to obtain seed liquid. The cells were collected by centrifugation.
The thalli is prepared into bacterial suspension by 5mL of sterile water, 1mL of the bacterial suspension is added into raw water of the black and odorous water body, and the mixture is placed at the temperature of 23 ℃ under the condition of 50 r/min. COD of the raw water of the black and odorous water body, ammonia nitrogen and total phosphorus concentration are measured every 24 hours, and COD reduction rate, ammonia nitrogen degradation rate and phosphorus removal rate are calculated, and the results are shown in Table 1.
TABLE 1 content of each index in water treated for different days
| CODCr/mg/L | Content of ammonia nitrogen/mg/L | Phosphorus content/mg/L | |
| 6h | 231.42 | 49.35 | 12.28 |
| 12h | 220.65 | 48.89 | 11.65 |
| 1 day | 196.81 | 47.05 | 10.58 |
| 3 days | 76.32 | 39.22 | 6.21 |
| 7 days | 23.52 | 24.93 | 5.10 |
| 15 days | 19.68 | 2.25 | 5.50 |
CODCrThe oxygen demand is measured by using potassium dichromate as an oxidant, and is a value of COD measured by using a potassium dichromate method.
As can be seen from table 1, COD decreased by 68.2%, 90.2% and 91.8% 3 days, 7 days and 15 days after inoculation, respectively; the ammonia nitrogen degradation rates of 10 days and 15 days after the inoculation of the strain are respectively 54.4 percent and 95.5 percent; the phosphorus removal rate reaches 60.8 percent 7 days after the inoculation of the strain.
The special degrading bacterium for the black and odorous water body and the application thereof are described in detail above. The principles and embodiments of the present invention are explained herein using specific examples, which are presented only to assist in understanding the method and its central concept. It should be noted that it would be apparent to those skilled in the art that various changes and modifications can be made in the invention without departing from the principles of the invention, and such changes and modifications are intended to be covered by the appended claims.
Claims (6)
1. The black and odorous water degradation strain is characterized by being bacillus megaterium with the preservation number of CGMCC No. 13543.
2. The use of the black and odorous water degrading strain according to claim 1, wherein the black and odorous water degrading strain is used for reducing COD in the black and odorous water, degrading ammonia nitrogen and/or removing phosphate in the black and odorous water, and the pH of the black and odorous water is 6.5 to 8.5; the temperature of the black and odorous water body is 10-40 ℃.
3. The use according to claim 2, wherein the black and odorous water body is from a river, a pond or a ditch.
4. The use according to claim 2, wherein the state of the black and odorous water body is standing, shaking or aeration.
5. The use according to claim 2, wherein the pH of the black and odorous water body is 7.5 to 8.2 and the temperature of the black and odorous water body is 23 to 30 ℃.
6. The use according to claim 2, wherein the state of the black and odorous water body is shaking or aeration.
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| CN110157631A (en) * | 2018-03-27 | 2019-08-23 | 新疆水处理工程技术研究中心有限公司 | A kind of heat-resisting dephosphorization composite functional bacterial agent and preparation method thereof |
| CN108977398B (en) * | 2018-09-13 | 2021-10-08 | 成都友益佳环保设备工程有限公司 | Bacillus megaterium and application thereof |
| CN109455823A (en) * | 2018-12-04 | 2019-03-12 | 扬州红硕环境与生物工程研究有限公司 | Phosphatic preparation and its preparation method and application in a kind of fixed water body |
| CN109402029B (en) * | 2018-12-20 | 2020-11-10 | 北京本农科技发展有限公司 | Separation and purification method of ammonia nitrogen degrading bacteria, ammonia nitrogen degrading bacteria and application |
| CN116463232B (en) * | 2022-01-11 | 2023-09-19 | 河北省科学院生物研究所 | Glutamine bacillus G2 and application thereof |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS537744B1 (en) * | 1971-09-11 | 1978-03-22 | ||
| RU2185339C2 (en) * | 2000-07-12 | 2002-07-20 | Тверской государственный технический университет | Method of biological cleaning of sewage from contaminants |
| US6562585B1 (en) * | 1997-06-03 | 2003-05-13 | William N. Hiatt | Beneficial bacterial preparation and method |
| CN101074421A (en) * | 2006-05-19 | 2007-11-21 | 中国石油化工股份有限公司 | Megacine and its use |
| KR100807219B1 (en) * | 2007-11-22 | 2008-02-28 | (주)비썬 | Purification apparatus and method for high organic wastewater |
| CN101255071A (en) * | 2007-03-01 | 2008-09-03 | 上海绿乐生物科技有限公司 | Method for producing phosphobacteria bio-fertilizer by using urban sludge |
| CN103468613A (en) * | 2013-09-12 | 2013-12-25 | 江苏省苏微微生物研究有限公司 | Bacillus megatherium, method for preparing microbial inoculum through solid fermentation of bacillus megatherium and application of microbial inoculum |
| CN103540549A (en) * | 2013-10-24 | 2014-01-29 | 哈尔滨工业大学 | Preparation method of compound microbial inoculum for degrading domestic sewage |
| CN104164388A (en) * | 2014-07-09 | 2014-11-26 | 青岛蔚蓝生物集团有限公司 | Bacillus megaterium and application thereof in aquaculture |
| CN105331554A (en) * | 2015-10-28 | 2016-02-17 | 北京沃土天地生物科技有限公司 | Compound microorganism bacterium agent and preparation method and application thereof |
| CN105950512A (en) * | 2016-06-03 | 2016-09-21 | 江南大学 | Microorganism bacterium agent for removing COD in sewage and preparation method and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7267772B2 (en) * | 2004-12-28 | 2007-09-11 | Council Of Scientific And Industrial Research | Biological process for reducing chemical and biochemical oxygen demand of pulp and paper industrial effluent |
-
2017
- 2017-09-13 CN CN201710821658.2A patent/CN107699513B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS537744B1 (en) * | 1971-09-11 | 1978-03-22 | ||
| US6562585B1 (en) * | 1997-06-03 | 2003-05-13 | William N. Hiatt | Beneficial bacterial preparation and method |
| RU2185339C2 (en) * | 2000-07-12 | 2002-07-20 | Тверской государственный технический университет | Method of biological cleaning of sewage from contaminants |
| CN101074421A (en) * | 2006-05-19 | 2007-11-21 | 中国石油化工股份有限公司 | Megacine and its use |
| CN101255071A (en) * | 2007-03-01 | 2008-09-03 | 上海绿乐生物科技有限公司 | Method for producing phosphobacteria bio-fertilizer by using urban sludge |
| KR100807219B1 (en) * | 2007-11-22 | 2008-02-28 | (주)비썬 | Purification apparatus and method for high organic wastewater |
| CN103468613A (en) * | 2013-09-12 | 2013-12-25 | 江苏省苏微微生物研究有限公司 | Bacillus megatherium, method for preparing microbial inoculum through solid fermentation of bacillus megatherium and application of microbial inoculum |
| CN103540549A (en) * | 2013-10-24 | 2014-01-29 | 哈尔滨工业大学 | Preparation method of compound microbial inoculum for degrading domestic sewage |
| CN104164388A (en) * | 2014-07-09 | 2014-11-26 | 青岛蔚蓝生物集团有限公司 | Bacillus megaterium and application thereof in aquaculture |
| CN105331554A (en) * | 2015-10-28 | 2016-02-17 | 北京沃土天地生物科技有限公司 | Compound microorganism bacterium agent and preparation method and application thereof |
| CN105950512A (en) * | 2016-06-03 | 2016-09-21 | 江南大学 | Microorganism bacterium agent for removing COD in sewage and preparation method and application thereof |
Non-Patent Citations (5)
| Title |
|---|
| 《Enhanced anaerobically digested swine wastewater treatment by the composite of polyaluminum chloride (PAC) and Bacillus megatherium G106 derived EPS》;Junyuan Guo等;《SCIENTIFIC REPORTS》;20170817;第7卷;参见对比文件1摘要、第7页"Discussion"、"Microorganism strain isolation and identifcation" * |
| 《Isolation, Identification, and Optimization of Culture Conditions of a Bioflocculant-Producing Bacterium Bacillus megaterium SP1 and Its Application in Aquaculture Wastewater Treatment》;Luo, Liang等;《BIOMED RESEARCH INTERNATIONAL》;20161020;全文 * |
| 《一株高效脱硫脱氨氮菌的分离、鉴定及系统发育分析》;张剑鸣等;《环境工程报》;20090531;第3卷(第5期);全文 * |
| 《巨大芽孢杆菌对养殖水体氨氮降解特性研究》;侯 颖等;《沈阳农业大学学报》;20060831;第37卷(第4期);全文 * |
| Luo, Liang;Zhao, Zhigang;Huang, Xiaoli等..《Isolation, Identification, and Optimization of Culture Conditions of a Bioflocculant-Producing Bacterium Bacillus megaterium SP1 and Its Application in Aquaculture Wastewater Treatment》.《BIOMED RESEARCH INTERNATIONAL》.2016, * |
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