CN103540549A - Preparation method of compound microbial inoculum for degrading domestic sewage - Google Patents
Preparation method of compound microbial inoculum for degrading domestic sewage Download PDFInfo
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Abstract
The invention discloses a preparation method of a compound microbial inoculum for degrading domestic sewage, relating to a preparation method of compound microbial inocula and solving the problem in the prior art of low nitrogen and phosphorus removal rate of sewage in artificial wetland at a low temperature environment. The preparation method comprises the following steps of: I, respectively inoculating pseudomonas, bacillus, nitrobacter, nitrosomonas and bacillus megatherium in a sterilized domestic sewage culture medium for culturing to obtain a respective inoculum; II, preparing COD (Chemical Oxygen Demand) degradable compound bacteria, preparing nitrobacter and nitrosomonas compound inocula, and taking the bacillus megatherium inoculum as phosphorous removal bacteria; and III, mixing the COD degradable compound bacteria, the nitrobacter and nitrosomonas compound inocula and the phosphorous removal bacteria under the condition of the same bacterial count. The prepared compound microbial inoculum for degrading domestic sewage is strong in adaptability to the environment, suitable for microorganism enhanced sewage treatment in the northern area, the ammonia nitrogen conversion rate of sewage can reach 95%, the total nitrogen conversion rate reaches 82%, the COD conversion rate reaches 60%, and the total phosphorus conversion rate is about 75%.
Description
Technical field
The present invention relates to a kind of preparation method of composite fungus agent.
Background technology
Along with the problem of water pollution is increasingly serious, effective control that water pollutes is very urgent.The essential substance of polluted-water is organism, total nitrogen and total phosphorus.Therefore, the removal of carbon, nitrogen, phosphorus is the key of water pollution control.In all sewage disposal technologies, biochemical process the most effectively, generally.Yet what routine biochemistry method was mainly removed is the organism in sewage, and the removal effect of nitrogen, phosphorus is existed to certain restriction.If the back segment in routine biochemistry method is aided with the advanced treatment to nitrogen, phosphorus, will certainly strengthen cost and the energy consumption of sewage disposal.So how, under minimum consumption, carbon, nitrogen, the phosphorus efficiently removed in sewage are one of Focal point and difficult points of current water pollution control area research.
Artificial swamp, as a kind of sewage disposal technology that can through engineering approaches, not only has the economic advantages such as investment in research and development is few, working cost is low, and its matrix, plant and the core system of the composition of the microorganism can realize the removal of synchronizeing of carbon in sewage, nitrogen, phosphorus.Wherein, matrix provides growing space for microorganism and plant, and can hold back the suspended matter in sewage.Plant absorbs the phosphoric acid salt of solubility in sewage, reaches the effect of dephosphorization by modes such as harvestings.Microorganism, by the synthetic and metabolism of self, reaches carbon, nitrogen, the synchronous effect of removing of phosphorus.Wherein, heterotroph microorganism is nutritive substance and energy by organic matter transformation, can remove the organic carbon in sewage.Ammonifying bacteria, nitrobacteria and denitrifying bacterium, by " ammonification-nitrated-denitrification " effect, remove the nitrogen element in sewage.Dephosphorization microorganism is converted into soluble phosphate by concerted reaction by organophosphorus and calcium phosphate precipitation, is absorbed, thereby remove the phosphoric in sewage by plant materials.Therefore, microorganism has play a part important in sewage purification process.
The operating performance of artificial marsh sewage treatment system is subject to the impact of two class conditions.First engineering specifications, as ratio of hydraulic detention time, organic loading, matrix and soil etc.; It two is natural condition, as geographical position, weather condition, temperature cycle etc.Wherein, low temperature is the most obvious on the impact of sewage disposal usefulness, is especially prone at northern area.Take the Northeast as example, no matter be plant, or be microorganism, due to the reduction of desmo enzyme activity, the ability of its conversion pollutant matter can decrease.Therefore, find effective technique means, break through temperature limitation, improve the sewage disposal usefulness of artificial swamp under cold condition and have great significance.
In actual artificial swamp engineering, find, when water temperature is lower, carbon in sewage, nitrogen, phosphorus still have removal to a certain degree, trace it to its cause, there is a small amount of cryophile in the system of being, this part microorganism not only can transform organic substance, completes denitrification reaction, even can realize the removal of part total phosphorus.If can screen this quasi-microorganism and build microbial inoculum, under cold operation condition, add in reactive system, just can reach the object of biological reinforced artificial swamp carbon, nitrogen, phosphorus removal.
From discussing above, in biochemical process sewage control technology, artificial swamp is a kind of effective selection.Yet the variation of temperature is the important factor that suppresses wet land system microbial performance, under especially northerly low temperature environment, the inhibition that microorganism is subject to is more obvious.So the microbial inoculum with carbon, nitrogen, phosphorus removal usefulness that structure can adapt to low temperature environment is the effective means of dealing with problems.
Therefore, by building effectively compound microbial inoculant, for the artificial wet land system under low temperature environment, improve structure of community and the diversity of microorganism in treatment system, improving the metabolic activity of microorganism, is the processing efficiency that improves sewage, reaches prerequisite and the guarantee of bioaugmentation.
Summary of the invention
The present invention seeks to solve the lower problem of artificial swamp sewerage nitrogen and phosphor clearance under existing low temperature environment, and a kind of preparation method of the sanitary sewage composite fungus agent of degrading is provided.
The preparation method of degraded sanitary sewage composite fungus agent, realizes according to the following steps:
One, pseudomonas, genus bacillus, bacterium nitrobacter, Nitrosomonas and bacillus megaterium are inoculated in respectively in the sanitary sewage substratum after sterilizing and are cultivated, in temperature, be 15 ℃, the bacterium number that is cultured to bacterium liquid under the condition that shaking speed is 130r/min reaches 3.6 * 10 successively
8～9.3 * 10
8individual/ml, 2.5 * 10
8～7.8 * 10
8individual/ml, 1.5 * 10
8～6.6 * 10
8individual/ml, 5.0 * 10
8～6.8 * 10
8individual/ml and 2.5 * 10
8～8.6 * 10
8individual/ml;
Two, the bacterium liquid of pseudomonas is mixed with the bacterium liquid of genus bacillus, obtain COD degraded composite bacteria, the bacterium liquid of bacterium nitrobacter is mixed with the bacterium liquid of Nitrosomonas, obtain nitrated nitrosification composite bacteria, the bacterium liquid of bacillus megaterium is except phosphorus bacteria;
Three, COD is degraded composite bacteria, nitrated nitrosification composite bacteria and except phosphorus bacteria is under the condition of identical bacterium number, according to volume ratio 10:(0.25～4): 0.5 mixes, and completes the preparation of degraded sanitary sewage composite fungus agent.
Degraded sanitary sewage composite fungus agent prepared by the present invention, tries out in the triangular flask of after containing sterilizing sanitary sewage and artificial swamp soil.Sewage ammonia nitrogen transformation efficiency can reach 95%, and total conversion rate of nitrogen reaches 82%, COD transformation efficiency and reaches 60%, and total phosphorus transformation efficiency can reach 75% left and right.
Degraded sanitary sewage composite fungus agent prepared by the present invention, not only artificial swamp sewage there is the degraded function of efficient stable, and strong to the adaptive capacity of environment, it is a kind of complex micro organism fungicide that economizes on resources, improves wastewater treatment efficiency, aspect the sewage disposal of northern area microbial augmentation, have broad prospect of application, its beneficial effect is:
(1) the degraded sanitary sewage composite fungus agent conversion usefulness that prepared by the present invention is good, and sewage ammonia nitrogen transformation efficiency can reach 95%, and total conversion rate of nitrogen reaches 82%, COD transformation efficiency and reaches 60%, and total phosphorus transformation efficiency can reach 75% left and right.
(2) easy to use, bacterium liquid is dropped into artificial wetland simulator soil surface, stop about 7 days.
(3) the degraded sanitary sewage composite fungus agent adaptive faculty that prepared by the present invention is stronger, makes it surrounding environment and can reach larger pH, temperature range.
(4) the degraded sanitary sewage composite fungus agent preparation process that prepared by the present invention is simple, and production cost is low, and environmental pollution is low.
Accompanying drawing explanation
Fig. 1 is that in embodiment, different volumes is than the mineralized nitrogen rate of lower obtained degraded sanitary sewage composite fungus agent, and wherein a represents initial value, and b represents stop value, ▲ expression clearance;
W represents to add in shaking flask the sanitary sewage after 120ml sterilizing;
W+S represents to add in shaking flask the sanitary sewage+10g soil after 120ml sterilizing;
W+S+0.25 represents to add in shaking flask the composite bacteria liquid of the sanitary sewage+10g soil+20ml volume ratio 10:0.25:0.5 after 100ml sterilizing;
W+S+0.5 represents to add in shaking flask the composite bacteria liquid of the sanitary sewage+10g soil+20ml volume ratio 10:0.5:0.5 after 100ml sterilizing;
W+S+1 represents to add in shaking flask the composite bacteria liquid of the sanitary sewage+10g soil+20ml volume ratio 10:1:0.5 after 100ml sterilizing;
W+S+2 represents to add in shaking flask the composite bacteria liquid of the sanitary sewage+10g soil+20ml volume ratio 10:2:0.5 after 100ml sterilizing;
W+S+4 represents to add in shaking flask the composite bacteria liquid of the sanitary sewage+10g soil+20ml volume ratio 10:4:0.5 after 100ml sterilizing;
Fig. 2 is that in embodiment, different volumes is than total conversion rate of nitrogen of lower obtained degraded sanitary sewage composite fungus agent, and wherein a represents initial value, and b represents stop value, ▲ expression clearance;
W represents to add in shaking flask the sanitary sewage after 120ml sterilizing;
W+S represents to add in shaking flask the sanitary sewage+10g soil after 120ml sterilizing;
W+S+0.25 represents to add in shaking flask the composite bacteria liquid of the sanitary sewage+10g soil+20ml volume ratio 10:0.25:0.5 after 100ml sterilizing;
W+S+0.5 represents to add in shaking flask the composite bacteria liquid of the sanitary sewage+10g soil+20ml volume ratio 10:0.5:0.5 after 100ml sterilizing;
W+S+1 represents to add in shaking flask the composite bacteria liquid of the sanitary sewage+10g soil+20ml volume ratio 10:1:0.5 after 100ml sterilizing;
W+S+2 represents to add in shaking flask the composite bacteria liquid of the sanitary sewage+10g soil+20ml volume ratio 10:2:0.5 after 100ml sterilizing;
W+S+4 represents to add in shaking flask the composite bacteria liquid of the sanitary sewage+10g soil+20ml volume ratio 10:4:0.5 after 100ml sterilizing;
Fig. 3 is that in embodiment, different volumes is than the total phosphorus transformation efficiency of lower obtained degraded sanitary sewage composite fungus agent, and wherein a represents initial value, and b represents stop value, ▲ expression clearance;
W represents to add in shaking flask the sanitary sewage after 120ml sterilizing;
W+S represents to add in shaking flask the sanitary sewage+10g soil after 120ml sterilizing;
W+S+0.25 represents to add in shaking flask the composite bacteria liquid of the sanitary sewage+10g soil+20ml volume ratio 10:0.25:0.5 after 100ml sterilizing;
W+S+0.5 represents to add in shaking flask the composite bacteria liquid of the sanitary sewage+10g soil+20ml volume ratio 10:0.5:0.5 after 100ml sterilizing;
W+S+1 represents to add in shaking flask the composite bacteria liquid of the sanitary sewage+10g soil+20ml volume ratio 10:1:0.5 after 100ml sterilizing;
W+S+2 represents to add in shaking flask the composite bacteria liquid of the sanitary sewage+10g soil+20ml volume ratio 10:2:0.5 after 100ml sterilizing;
W+S+4 represents to add in shaking flask the composite bacteria liquid of the sanitary sewage+10g soil+20ml volume ratio 10:4:0.5 after 100ml sterilizing;
Fig. 4 is that in embodiment, different volumes is than the COD transformation efficiency of lower obtained degraded sanitary sewage composite fungus agent, and wherein curve 1 represents to add the sanitary sewage after 120ml sterilizing in shaking flask;
Embodiment
Technical solution of the present invention is not limited to following cited embodiment, also comprises the arbitrary combination between each embodiment.
Embodiment one: the preparation method of present embodiment degraded sanitary sewage composite fungus agent, realizes according to the following steps:
One, pseudomonas, genus bacillus, bacterium nitrobacter, Nitrosomonas and bacillus megaterium are inoculated in respectively in the sanitary sewage substratum after sterilizing and are cultivated, in temperature, be 15 ℃, the bacterium number that is cultured to bacterium liquid under the condition that shaking speed is 130r/min reaches 3.6 * 10 successively
8～9.3 * 10
8individual/ml, 2.5 * 10
8～7.8 * 10
8individual/ml, 1.5 * 10
8～6.6 * 10
8individual/ml, 5.0 * 10
8～6.8 * 10
8individual/ml and 2.5 * 10
8～8.6 * 10
8individual/ml;
Two, the bacterium liquid of pseudomonas is mixed with the bacterium liquid of genus bacillus, obtain COD degraded composite bacteria, the bacterium liquid of bacterium nitrobacter is mixed with the bacterium liquid of Nitrosomonas, obtain nitrated nitrosification composite bacteria, the bacterium liquid of bacillus megaterium is except phosphorus bacteria;
Three, COD is degraded composite bacteria, nitrated nitrosification composite bacteria and except phosphorus bacteria is under the condition of identical bacterium number, according to volume ratio 10:(0.25～4): 0.5 mixes, and completes the preparation of degraded sanitary sewage composite fungus agent.
In present embodiment step 2, the bacterium liquid of pseudomonas being mixed with the bacterium liquid of genus bacillus, is than mixing according to any; The bacterium liquid of bacterium nitrobacter is mixed with the bacterium liquid of Nitrosomonas, is than mixing according to any.
In present embodiment, pseudomonas, genus bacillus, bacterium nitrobacter, Nitrosomonas and bacillus megaterium are to buy and obtain.
Embodiment two: present embodiment is different from embodiment one, in step 1 sanitary sewage substratum, the sterilising conditions of sewage is 121 ℃, 30min.Other step and parameter are identical with embodiment one.
Embodiment three: present embodiment is different from embodiment one or two, the composite bacteria of in step 3, COD being degraded, nitrated nitrosification composite bacteria and except phosphorus bacteria under the condition of identical bacterium number, according to volume ratio, 10:0.25:0.5 mixes.Other step and parameter are identical with embodiment one or two.
Embodiment four: present embodiment is different from embodiment one or two, the composite bacteria of in step 3, COD being degraded, nitrated nitrosification composite bacteria and except phosphorus bacteria under the condition of identical bacterium number, according to volume ratio, 10:0.5:0.5 mixes.Other step and parameter are identical with embodiment one or two.
Embodiment five: present embodiment is different from embodiment one or two, the composite bacteria of in step 3, COD being degraded, nitrated nitrosification composite bacteria and except phosphorus bacteria under the condition of identical bacterium number, according to volume ratio, 10:1:0.5 mixes.Other step and parameter are identical with embodiment one or two.
Embodiment six: present embodiment is different from embodiment one or two, the composite bacteria of in step 3, COD being degraded, nitrated nitrosification composite bacteria and except phosphorus bacteria under the condition of identical bacterium number, according to volume ratio, 10:2:0.5 mixes.Other step and parameter are identical with embodiment one or two.
Embodiment seven: present embodiment is different from embodiment one or two, the composite bacteria of in step 3, COD being degraded, nitrated nitrosification composite bacteria and except phosphorus bacteria under the condition of identical bacterium number, according to volume ratio, 10:4:0.5 mixes.Other step and parameter are identical with embodiment one or two.
Embodiment:
The preparation method of degraded sanitary sewage composite fungus agent, realizes according to the following steps:
One, pseudomonas, genus bacillus, bacterium nitrobacter, Nitrosomonas and bacillus megaterium are inoculated in respectively in the sanitary sewage substratum after sterilizing and are cultivated, in temperature, be 15 ℃, the bacterium number that is cultured to bacterium liquid under the condition that shaking speed is 130r/min reaches 3.6 * 10 successively
8～9.3 * 10
8individual/ml, 2.5 * 10
8～7.8 * 10
8individual/ml, 1.5 * 10
8～6.6 * 10
8individual/ml, 5.0 * 10
8～6.8 * 10
8individual/ml and 2.5 * 10
8～8.6 * 10
8individual/ml;
Two, the bacterium liquid of pseudomonas is mixed with the bacterium liquid of genus bacillus, obtain COD degraded composite bacteria, the bacterium liquid of bacterium nitrobacter is mixed with the bacterium liquid of Nitrosomonas, obtain nitrated nitrosification composite bacteria, the bacterium liquid of bacillus megaterium is except phosphorus bacteria;
Three, COD is degraded composite bacteria, nitrated nitrosification composite bacteria and except phosphorus bacteria is under the condition of identical bacterium number, according to volume ratio 10:(0.25～4): 0.5 mixes, and completes the preparation of degraded sanitary sewage composite fungus agent.
In the present embodiment, pseudomonas, genus bacillus, bacterium nitrobacter, Nitrosomonas and bacillus megaterium are to buy and obtain.
In the present embodiment respectively according to volume ratio 10:0.25:0.5,10:0.5:0.5,10:1:0.5,10:2:0.5,10:4:0.5 mix the degraded sanitary sewage composite fungus agent of rear preparation, carry out shake flat experiment, add in the triangular flask of sanitary sewage after containing sterilizing (120ml) and artificial swamp soil (10g), and do not add bacteria agent soil effect comparison:
As shown in Figure 1, under the condition that composite fungus agent is 10:0.5:0.5 in volume ratio, mineralized nitrogen rate is the highest for ammonia nitrogen result, reaches more than 95%, and blank group mineralized nitrogen rate is 70%, and experimental result is better than blank group;
Total nitrogen result as shown in Figure 2, when composite fungus agent total conversion rate of nitrogen under the condition of volume ratio 10:0.5:0.5 is the highest, reaches 80%, and the total conversion rate of nitrogen of blank group is 60%, and experimental result is better than blank group;
As shown in Figure 3, when composite fungus agent total phosphorus transformation efficiency under each volume ratio condition is more or less the same, all in 75% left and right, blank group total phosphorus transformation efficiency is 70% to total phosphorus result, and experimental result is better than blank group.
As shown in Figure 4, when composite fungus agent COD transformation efficiency under each volume ratio condition is more or less the same, all in 60% left and right, blank group is 50% at first three day COD transformation efficiency to COD result, and experimental result is better than blank group.Yet, along with the carrying out of reaction, with microbial inoculum combination COD transformation efficiency, reduce gradually, therefore, really when adding microbial inoculum in artificial swamp, can consider that short-term repeatedly adds COD degradation bacteria, meanwhile, this experiment is also for engineering operation provides theoretical direction.
Claims (7)
1. the degrade preparation method of sanitary sewage composite fungus agent, is characterized in that it realizes according to the following steps:
One, pseudomonas, genus bacillus, bacterium nitrobacter, Nitrosomonas and bacillus megaterium are inoculated in respectively in the sanitary sewage substratum after sterilizing and are cultivated, in temperature, be 15 ℃, the bacterium number that is cultured to bacterium liquid under the condition that shaking speed is 130r/min reaches 3.6 * 10 successively
8～9.3 * 10
8individual/ml, 2.5 * 10
8～7.8 * 10
8individual/ml, 1.5 * 10
8～6.6 * 10
8individual/ml, 5.0 * 10
8～6.8 * 10
8individual/ml and 2.5 * 10
8～8.6 * 10
8individual/ml;
Two, the bacterium liquid of pseudomonas is mixed with the bacterium liquid of genus bacillus, obtain COD degraded composite bacteria, the bacterium liquid of bacterium nitrobacter is mixed with the bacterium liquid of Nitrosomonas, obtain nitrated nitrosification composite bacteria, the bacterium liquid of bacillus megaterium is except phosphorus bacteria;
Three, COD is degraded composite bacteria, nitrated nitrosification composite bacteria and except phosphorus bacteria is under the condition of identical bacterium number, according to volume ratio 10:(0.25～4): 0.5 mixes, and completes the preparation of degraded sanitary sewage composite fungus agent.
2. the preparation method of a kind of sanitary sewage composite fungus agent of degrading according to claim 1, the sterilising conditions that it is characterized in that sewage in step 1 sanitary sewage substratum is 121 ℃, 30min.
3. the preparation method of a kind of sanitary sewage composite fungus agent of degrading according to claim 2, composite bacteria is characterized in that in step 3, COD being degraded, nitrated nitrosification composite bacteria and except phosphorus bacteria under the condition of identical bacterium number, according to volume ratio, 10:0.25:0.5 mixes.
4. the preparation method of a kind of sanitary sewage composite fungus agent of degrading according to claim 2, composite bacteria is characterized in that in step 3, COD being degraded, nitrated nitrosification composite bacteria and except phosphorus bacteria under the condition of identical bacterium number, according to volume ratio, 10:0.5:0.5 mixes.
5. the preparation method of a kind of sanitary sewage composite fungus agent of degrading according to claim 2, composite bacteria is characterized in that in step 3, COD being degraded, nitrated nitrosification composite bacteria and except phosphorus bacteria under the condition of identical bacterium number, according to volume ratio, 10:1:0.5 mixes.
6. the preparation method of a kind of sanitary sewage composite fungus agent of degrading according to claim 2, composite bacteria is characterized in that in step 3, COD being degraded, nitrated nitrosification composite bacteria and except phosphorus bacteria under the condition of identical bacterium number, according to volume ratio, 10:2:0.5 mixes.
7. the preparation method of a kind of sanitary sewage composite fungus agent of degrading according to claim 2, composite bacteria is characterized in that in step 3, COD being degraded, nitrated nitrosification composite bacteria and except phosphorus bacteria under the condition of identical bacterium number, according to volume ratio, 10:4:0.5 mixes.
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Cited By (8)
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| CN106630190A (en) * | 2016-12-20 | 2017-05-10 | 深圳市禹邦水处理技术有限公司 | COD (chemical oxygen demand)-degrading fungicide agent and preparation method thereof |
| CN106745811A (en) * | 2016-12-29 | 2017-05-31 | 安徽佳明环保科技股份有限公司 | A kind of composition and its preparation and application for processing sanitary sewage |
| CN106947709A (en) * | 2016-01-07 | 2017-07-14 | 上海中海龙智城科技有限公司 | A kind of complex microbial community of degradation of ammonia nitrogen |
| CN107699513A (en) * | 2017-09-13 | 2018-02-16 | 博元生态修复（北京）有限公司 | A kind of special degradation bacteria of black and odorous water and its application |
| CN109370957A (en) * | 2018-12-10 | 2019-02-22 | 江南大学 | A kind of sewage treatment composite bacteria agent and preparation method thereof |
| CN109609411A (en) * | 2019-01-08 | 2019-04-12 | 北京科技大学 | A kind of microbial flora and preparation method thereof for erythromycin of degrading |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106947709A (en) * | 2016-01-07 | 2017-07-14 | 上海中海龙智城科技有限公司 | A kind of complex microbial community of degradation of ammonia nitrogen |
| CN106630190A (en) * | 2016-12-20 | 2017-05-10 | 深圳市禹邦水处理技术有限公司 | COD (chemical oxygen demand)-degrading fungicide agent and preparation method thereof |
| CN106630190B (en) * | 2016-12-20 | 2020-06-16 | 永旌(湖北)环境技术有限公司 | Fungus agent for degrading COD and preparation method thereof |
| CN106745811A (en) * | 2016-12-29 | 2017-05-31 | 安徽佳明环保科技股份有限公司 | A kind of composition and its preparation and application for processing sanitary sewage |
| CN107699513A (en) * | 2017-09-13 | 2018-02-16 | 博元生态修复（北京）有限公司 | A kind of special degradation bacteria of black and odorous water and its application |
| CN107699513B (en) * | 2017-09-13 | 2020-08-21 | 博元生态修复（北京）有限公司 | Black and odorous water body degrading bacterium and application thereof |
| CN110157631A (en) * | 2018-03-27 | 2019-08-23 | 新疆水处理工程技术研究中心有限公司 | A kind of heat-resisting dephosphorization composite functional bacterial agent and preparation method thereof |
| CN109370957A (en) * | 2018-12-10 | 2019-02-22 | 江南大学 | A kind of sewage treatment composite bacteria agent and preparation method thereof |
| CN109609411A (en) * | 2019-01-08 | 2019-04-12 | 北京科技大学 | A kind of microbial flora and preparation method thereof for erythromycin of degrading |
| CN110468081A (en) * | 2019-09-17 | 2019-11-19 | 湖南艾布鲁环保科技股份有限公司 | Composite bacteria agent and its preparation method and application |
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Application publication date: 20140129 |