CN106947709A - A kind of complex microbial community of degradation of ammonia nitrogen - Google Patents
A kind of complex microbial community of degradation of ammonia nitrogen Download PDFInfo
- Publication number
- CN106947709A CN106947709A CN201610868376.3A CN201610868376A CN106947709A CN 106947709 A CN106947709 A CN 106947709A CN 201610868376 A CN201610868376 A CN 201610868376A CN 106947709 A CN106947709 A CN 106947709A
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- Prior art keywords
- ammonia nitrogen
- degradation
- pseudomonas
- microbial community
- complex microbial
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/007—Contaminated open waterways, rivers, lakes or ponds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The microorganism species of ammonia nitrogen degradation, including nitrification bacterial strain Nitrosomas category can be carried out at low ambient temperatures it is an object of the invention to provide a kind of（Nitrosomonas）, pseudomonas（Pseudomonas）, bacillus（Bacillus）With nitric acid Pseudomonas（Nitrobacter）.The present invention is separated from activated sludge, the ammonia nitrogen degradation microorganism species that filter out can carry out ammonia nitrogen degradation in low-temperature sewage.10 are reached in bacterial strain amount9~1011Cfu/ml, when initial ammonia nitrogen concentration is 5.0mg/L, addition sodium acetate is as carbon source, and C/N ratios are 2, in 2 DEG C of temperature, pH value 7.2, and under the conditions of 150rpm (dissolved oxygen is more than 2mg/L), after ammonia nitrogen degradation 30min, degradation rate is up to 9.350mg NH4+/L·h.Compared with traditional single culture, the present invention can greatly improve the degradation rate of ammonia nitrogen in sewage, and high-quality and efficient strain is provided for sewage disposal.
Description
Technical field
The present invention belongs to microorganism field for a kind of complex microbial community, is related to the ammonia nitrogen drop in sewage disposal process
Solution.
Background technology
The main process of sewage treatment plant of China and sewage disposal device is biologic treating technique at present, is microorganism creation
Good environmental condition, enables microorganism that the harmful substance in sewage effectively to be degraded.Temperature largely influences micro-
Biological physiological activity, so as to influence the effect of sewage disposal.According to investigation, cold district sewage handling problem, which drastically influence, works as
The quality of life of ground people and economic development.The growth of any microorganism has a suitable temperature, in certain temperature range
Interior, with the rising of temperature, growth of microorganism accelerates, and after temperature is reduced to a certain extent, growth of microorganism is very slow, very
To stopping growing.And wherein nitrifier is sensitive to environmental condition, optimum growth temperature is 20~30 DEG C, when less than 15 DEG C, nitre
Change speed to decline, it is difficult to adapt to low temperature environment, greatly have impact on the effect of sewage disposal, therefore invention can be under low-temperature condition
The flora of degraded ammonia nitrogen in sewage possesses significance.
The content of the invention
The present invention is intended to provide a kind of apply convenient microbial composite bacteria group to solve in sewage at low temperatures
The problem of ammonia nitrogen degradation is difficult.The ammonia nitrogen degradation in sewage can not only be applied to, additionally it is possible to be widely used in improvement, the fish in river
Purification of class breeding water body etc..
The present invention is achieved by the following technical programs.
Convenient microbial composite bacteria group is applied to solve sewage at low temperatures it is an object of the invention to provide one kind
In the difficult above mentioned problem of ammonia nitrogen degradation.
Inventor is screened by studying for a long time by single factor experiment, determines the optimal strain group of complex microorganism
Various strain ratios and preparation method in conjunction, flora.
The present invention is used for the complex microbial community of ammonia nitrogen degradation, includes nitrification bacterial strain Nitrosomas category, pseudomonad
Category, bacillus and nitric acid Pseudomonas.
In above-mentioned complex microbial community, nitrification bacterial strain Nitrosomas category, pseudomonas, bacillus and nitric acid
The strain quantity ratio of Pseudomonas is 2:1:2:1；
Present invention optimizes optimal strain combination, optimal strain ratio, its ammonia nitrogen degradation effect is significantly better than traditional single bacterium
Plant and the composite bacteria without proportioning, ammonia nitrogen degradation efficiency can be greatly improved, solve the problem of ammonia nitrogen degradation under cryogenic conditions.
Embodiment
The present invention, which have passed through to study for a long period of time, determines a kind of strain combination for ammonia nitrogen degradation, its composite degradation bacterial strain by
Bacterial strain Nitrosomas category, pseudomonas, bacillus and nitric acid Pseudomonas composition are nitrified, passes through answering between above-mentioned four kinds of bacterium
Carry out effective degradation of ammonia nitrogen with effect.
Winter Song Hua River river bed sludge 5ml is taken, is seeded in 50ml screening and culturing mediums, at 2 DEG C, under the conditions of 150rpm/min
It is enriched with after 10d, gradient dilution to be inoculated on solid medium and cultivates 5 days, chooses typical single bacterium colony and carry out purifying culture, will
The pure culture bacterial strain finally obtained ammonia nitrogen degradation speed is reached in ammonia nitrogen degradation experiment, selection 30min more than 50% bacterium
Strain, reference《Common bacteria system identification handbook》Isolate and obtain about 25 plants of bacterium of 8 kinds of Pseudomonas.
Only nitrification bacterial strain Nitrosomas category, pseudomonas, bacillus and Nitromonas are found by mutually proportioning
The complex microbial community for belonging to composition is best to the degradation effect of ammonia nitrogen, and its strain quantity ratio is 2:1:2:1.
Obtain using effect by contrasting flora of the present invention and common ammonia nitrogen degradation flora and compare as follows：
| Temperature（℃） | 30 | 20 | 10 | 2 |
| Common ammonia nitrogen degradation bacterium 30min degradation rates | 25.375 | 13.605 | 1.254 | 0.020 |
| Ammonia nitrogen degradation bacterium 30min degradation rate mg NH of the present invention4+/L·h | 15.364 | 12.533 | 10.542 | 9.350 |
Can be seen that by upper table, flora of the present invention under cryogenic the relatively common ammonia nitrogen degradation bacterium of degradation of ammonia nitrogen effect have it is bright
Aobvious advantage.
Claims (4)
1. a kind of complex microbial community, it is characterised in that described complex microbial community includes nitrification bacterial strain Nitrosomas
Category, pseudomonas, bacillus and nitric acid Pseudomonas.
2. complex microbial community as claimed in claim 1, it is characterised in that described complex microbial community has nitrifier
Strain nitrous acid Pseudomonas, pseudomonas, bacillus and nitric acid Pseudomonas composition.
3. complex microbial community as claimed in claim 1, it is characterised in that described nitrification bacterial strain Nitrosomas belongs to, vacation
The quantity ratio of zygosaccharomyces, bacillus and nitric acid Pseudomonas is 2:1:2:1.
4. application of the complex microbial community in sewage disposal ammonia nitrogen degradation described in any one of claim 1 ~ 3.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2016200106829 | 2016-01-07 | ||
| CN201620010682 | 2016-01-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106947709A true CN106947709A (en) | 2017-07-14 |
Family
ID=59466120
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610868376.3A Pending CN106947709A (en) | 2016-01-07 | 2016-09-30 | A kind of complex microbial community of degradation of ammonia nitrogen |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106947709A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109231476A (en) * | 2017-07-11 | 2019-01-18 | 温月丽 | A kind of synthesis and application of the agent of novel ammonia nitrogen absorption degradation |
| CN112794455A (en) * | 2019-11-14 | 2021-05-14 | 东莞市蓝绅环保有限公司 | Sewage treatment agent for removing ammonia nitrogen and total nitrogen and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1270084A (en) * | 1999-04-14 | 2000-10-18 | 秦仁燮 | Method and apparatus for treating food waste by using microorgans at low and normal temperature |
| CN103540549A (en) * | 2013-10-24 | 2014-01-29 | 哈尔滨工业大学 | Preparation method of compound microbial inoculum for degrading domestic sewage |
-
2016
- 2016-09-30 CN CN201610868376.3A patent/CN106947709A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1270084A (en) * | 1999-04-14 | 2000-10-18 | 秦仁燮 | Method and apparatus for treating food waste by using microorgans at low and normal temperature |
| CN103540549A (en) * | 2013-10-24 | 2014-01-29 | 哈尔滨工业大学 | Preparation method of compound microbial inoculum for degrading domestic sewage |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109231476A (en) * | 2017-07-11 | 2019-01-18 | 温月丽 | A kind of synthesis and application of the agent of novel ammonia nitrogen absorption degradation |
| CN109231476B (en) * | 2017-07-11 | 2022-04-15 | 温月丽 | Synthesis and application of novel ammonia nitrogen adsorption degradation agent |
| CN112794455A (en) * | 2019-11-14 | 2021-05-14 | 东莞市蓝绅环保有限公司 | Sewage treatment agent for removing ammonia nitrogen and total nitrogen and preparation method thereof |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| CB02 | Change of applicant information |
Address after: No. 400 Gongqing Road, Yangpu District, Shanghai 200090 Applicant after: Shanghai sea dragon city Polytron Technologies Inc Address before: No. 400 Gongqing Road, Yangpu District, Shanghai 200090 Applicant before: Shanghai sea dragon Zhicheng Technology Co. Ltd. |
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| CB02 | Change of applicant information | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170714 |
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| WD01 | Invention patent application deemed withdrawn after publication |