CN102276124B - Microbe dredging agent and preparation method thereof - Google Patents

Microbe dredging agent and preparation method thereof Download PDF

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Publication number
CN102276124B
CN102276124B CN 201110142289 CN201110142289A CN102276124B CN 102276124 B CN102276124 B CN 102276124B CN 201110142289 CN201110142289 CN 201110142289 CN 201110142289 A CN201110142289 A CN 201110142289A CN 102276124 B CN102276124 B CN 102276124B
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desilting
bacterium
agent
liquid
mikrobe
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CN102276124A (en
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郭大磊
陶卫锋
黄学军
孙玉华
邵晓梅
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Kunshan Huake Membrane and Environmental Technology Co., Ltd.
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Huake Institute of Bio-polymeric Materials Kunshan Industrial Technology Resear
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Abstract

The invention relates to a microbe dredging agent. A porous zeolite is employed as a carrier; and mixed bacteria of actinomycetes, nitrosomonas eutropha, nitrobacter winogradskyi, paracoccus pantotrophus and bacillus megaterium, in a certain proportion, are used as dredging bacteria. The five specific dredging bacteria form a ''cell family'', which, through mutual conversion metabolism, not only can play a role of water purification in an oxygen-rich water area, but also can play a role of biological decomposition and conversion in a low-oxygen even anoxic environment (e.g. water-bed mud) and salty water area (e.g. gulf), so as to purify sewage and improve water quality. The invention employs a porous zeolite with a high absorptivity as a fixed carrier of the dredging bacteria, so that the dredging bacteria are more firmly fixed and not easily flushed by water; because the zeolite is apt to sinking, the microbe dredging agent of the invention is especially suitable for water-bed dredging of river and lake, etc. In addition, the preparation method of the microbe dredging agent of the invention has mild conditions, a simple technology and advantages of low production costs and benefit for popularization and application.

Description

A kind of mikrobe desilting agent and preparation method thereof
Technical field
The invention belongs to the environmental protection technical field of mikrobe, be specifically related to a kind of mikrobe desilting agent and preparation method thereof
Background technology
microbial water-purifying agent is a kind of preparation that utilizes microbially decontaminate sewage, and it generally is will be fixed on the specific carrier the microbially decontaminate flora that sewage has a Water purification through absorption method, covalent attachment method, crosslinking or entrapping method etc. to process.General microbial water-purifying agent is applicable to the sewage purification in breed waters such as pond among a small circle etc.; For mobile waters; The particularly water-bed desilting in mobile waters; Common microbial water-purifying agent is difficult to play a role (microbial water-purifying agent that is applied to water-bed desilting can be referred to as mikrobe desilting agent), its reason mainly contain following some: the purification flora that 1, uses basically all is an aerobic bacteria, like subtilis etc.; This makes the environment that dissolved oxygens such as water-bed mud are few or almost do not have dissolved oxygen, and the microbially decontaminate flora is difficult to bring into play bed mud desilting and water purification effect.Though 2, can come to bring into play to greatest extent the effectiveness that purifies flora through setting up aerating apparatus in the waters among a small circle, set up aerating apparatus and be difficult to realize for flow the on a large scale polluted-water in waters such as river, lake for cultivating pool etc.3, adopt usual way fixed microbially decontaminate flora; The performance water purification effect that suspends in water after generally spreading in the water, for the bed mud desilting in the waters of flowing, common microbial water-purifying agent can not sink to the bottom; Run off with current easily, thereby can not play the effect of desilting agent; And use mechanical desilting in the river course etc., not only cost is high, also can destroy ecotope, influences the self-purification function in waters
Summary of the invention
the present invention is intended to overcome the deficiency of prior art, and a kind of mikrobe desilting agent that can be applicable to mobile waters bed mud desilting is provided.
the present invention also will provide a kind of preparation method of mentioned microorganism desilting agent.
For solving above technical problem, the technical scheme that the present invention takes is:
a kind of mikrobe desilting agent; It is a carrier with the porous zeolite particle, to be pressed mixed bacterium that viable bacteria content proportioning 1.8~2.2:0.8~1.2:0.8~1.2:1.8~2.2:2.8~3.2 constitutes by actinomycetes (Actinomycetes), Nitrosomonas europaea (Nitrosomonas eutropha), Vickers bacterium nitrobacter (Nitrobacter winogradskyi), general foster secondary coccus (Paracoccus pantotrophus) and bacillus megaterium (Bacillus megaterium) as the desilting bacterium.
Actinomycetes (Actinomycetes) in the mentioned microorganism desilting agent and bacillus megaterium (Bacillus megaterium) can transfer organism in the mud or the decomposition of plant-animal septic matter small-molecule substances such as to amino acid, ammonia nitrogen, nitrate salt;
Nitrosomonas europaea (Nitrosomonas eutropha) is the Nitromonas kind; Can under aerobic fully or anoxia condition, carry out metabolism, its metabolic process is the nitrosification process, mainly is that the ammonia nitriding compound is oxidized to nitrite; Follow anti-nitration reaction simultaneously, and with N 2 Be final product;
Vickers bacterium nitrobacter (Nitrobacter winogradskyi) belongs to Nitrobacter, and its metabolic process is a nitrifying process, can be nitrate salt with nitrite-oxidizing, and it is a kind of chemoautotrophic bacteria;
General foster secondary coccus (Paracoccus pantotrophus) is a paracoccus, and it can become sulfate radical with sulfide oxidation effectively, and with itrated compound as electron acceptor(EA), the itrated compound denitrification is converted into nitrogen, its reaction formula is following:
CO 2 / CHO+H 2 S+NO 3 - → Biomass+SO4 2- + N 2 + Q (energy).
these five kinds of desilting bacterium constitute one " cell family ", and are mutually beneficial complementary, can be applied to the water purification in anaerobic environment and multiple different quality waters, and they can be clean with water-bed silt displacement through transforming metabolism, improves water quality.
preferably, said porous zeolite is that size is at 40 orders~250 purpose porous sodium type active zeolites.
preferably, said mikrobe desilting agent also has and is coated on the outer protective material of said carrier and desilting bacterium, and this protective material is preferably dextrin.
The preparation method of mentioned microorganism desilting agent comprises following several steps:
(1) activation treatment of zeolite: clinoptilolite is handled with excessive sodium salt solution, made Na + Exchange rate descended dry 2~3 hours at 90 ℃~120 ℃ more than 75% then, again 400 ℃~600 ℃ following heat activation 2~3 hours, obtained porous sodium type active zeolite particle;
(2) individual plant of desilting bacterium is cultivated: comprise that inoculation, fermentation culture and fermented liquid concentrate, and obtain the liquid concentrator of five kinds of desilting bacterium at last;
(3) mixing of desilting bacterium liquid concentrator: the liquid concentrator of step (2) gained actinomycetes, Nitrosomonas europaea, Vickers bacterium nitrobacter, general foster secondary coccus and bacillus megaterium is mixed by the viable bacteria content proportioning of 1.8~2.2:0.8~1.2:0.8~1.2:1.8~2.2:2.8~3.2, obtain mixed bacterium seed liquid;
(4) solidify cultivation: under aseptic condition; The porous sodium type active zeolite particle of step (3) gained mixed bacterium seed liquid and step (1) gained is even according to the mixed of weight ratio 1/19~1/4, be to solidify under 20 ℃~35 ℃, the condition of pH value to cultivate 12~18 hours in temperature then 6.5~7.5;
(5) drying: the porous sodium type active zeolite particulate surfaces coated through step (4) expires dextrin; Zeolite granular and appendix mixed bacterium is above that coated by dextrin fully; And under 30 ℃~50 ℃ temperature condition, carry out drying; Moisture content≤10% in said porous sodium type active zeolite particle, the dry completion obtains said mikrobe desilting agent.
are as preparing method's of the present invention preferred version; Fermentation culture process in the step (2) is: making five kinds of liquid nutrient mediums inoculating is that 30 ℃~37 ℃, pH value are to cultivate 14~24 hours under 6.5~8 the condition in temperature, obtains five kinds of fermented liquids.
Fermented liquid concentration process in step (2) is: said five kinds of fermented liquids are carried out ultrafiltration and concentration respectively, and obtaining the desilting bacteria concentration is five kinds of liquid concentrators of 2.5 times of desilting bacteria concentration in the fermented liquid.
in addition, used liquid nutrient medium, pH value regulator and other apparatus or reagent all pass through high pressure steam sterilization among the preparation method of the present invention.
Through the enforcement of above technical scheme, mikrobe desilting agent of the present invention is compared with microbial water-purifying agent of the prior art is had plurality of advantages:
mikrobe desilting agent of the present invention adopts five kinds of specific desilting bacterium to constitute one " cell family " according to a certain percentage; Through their mutual conversion metabolism; Not only can bring into play the water purification effect in the oxygen enrichment waters; Can also purify waste water in hypoxemia even anaerobic environment (like water-bed mud) and salt water waters (like the bay) performance biolysis transformation, improve water quality.Employing has the immobilization carrier of the porous zeolite of high absorbability as the desilting bacterium, makes the fixing more firm of desilting bacterium, and mikrobe desilting bacterium is difficult for being washed away by current; And the porous active zeolite also has certain slow releasing function, can prolong the effectiveness time of mikrobe desilting agent of the present invention; Because zeolite is easy to sink to the bottom, so mikrobe desilting agent of the present invention especially is fit to the water-bed desilting in river course, lake etc.In addition, preparing method's technology of mikrobe desilting agent of the present invention is simple, mild condition, and production cost is low.
Embodiment
are done further detailed explanation below in conjunction with concrete embodiment to the present invention, but are not limited to these embodiment.
Embodiment 1
present embodiment mikrobe desilting agent selects for use actinomycetes, Nitrosomonas europaea, Vickers bacterium nitrobacter, general foster secondary coccus and bacillus megaterium as the desilting bacterium, and preferred dimension is at the immobilization carrier of 40 orders~250 purpose porous sodium type active zeolites as the desilting bacterium.
Present embodiment mainly carries out screening and the seed liquor preparation work of above-mentioned five kinds of desilting bacterium:
because mikrobe is different, metabolic capacity is different, and growth velocity is different, if mixed culture can produce the growth competition, and microbiological contamination easily, so the cultivation of mikrobe must be cultivated by individual plant.The preparation process of above-mentioned five kinds of desilting bacterium seed liquor is:
are inserted five kinds of desilting bacterium respectively on five kinds of screening culture medium, place 35 ℃~37 ℃ incubators to cultivate 48h, filter out this five kinds of desilting bacterium then; On the test tube slant of beef extract-peptone basic medium, insert the above-mentioned desilting bacterium that filters out, place 35 ℃~37 ℃ incubators to cultivate 48h; On above-mentioned slant medium, access 2 ring lawns with transfering loop, be linked in the liquid seed culture medium,, under 35 ℃~37 ℃ the condition, cultivate 12~14h, make five kinds of desilting bacterium seed liquor at shaking speed 200~270rpm.Wherein, said liquid seed culture medium mainly be according to different bacterial classifications the nutritive medium that contains carbon source, nitrogenous source and inorganic salt of corresponding preparation.
Embodiment 2
Present embodiment selects for use embodiment 1 prepared desilting bacterium seed liquor to prepare mikrobe desilting agent, and it comprises the steps:
(1) pre-treatment of zeolite: clinoptilolite (can be NaCl, Na with excessive sodium salt solution 2 SO 4 , NaNO 3 Deng) handle, make Na + Exchange rate descended dry 2~3 hours at 90 ℃~120 ℃ more than 75% then, again 400 ℃~600 ℃ following heat activation 2~3 hours, obtained porous sodium type active zeolite particle; This kind active zeolite has improved the loading capacity to gas greatly, even also bigger than the adsorptive capacity of synthetic 5A molecular sieve.
(2) sterilization: five kinds of liquid nutrient mediums, the pH value regulator (ammoniacal liquor that will cultivate said actinomycetes, Nitrosomonas europaea, Vickers bacterium nitrobacter, general foster secondary coccus and bacillus megaterium respectively; Hydrochloric acid) and other apparatus and reagent carry out (121 ℃ of high pressure; 0.1Mpa) steam sterilizing, be cooled to 25 ℃~37 ℃ then; Five kinds of liquid nutrient mediums should be identical with the composition of aforesaid liquid seed culture medium here, and are just larger, cultivate the desilting bacterium and prepare for large-scale.
(3) inoculation: the seed liquor of said actinomycetes, Nitrosomonas europaea, Vickers bacterium nitrobacter, general foster secondary coccus and bacillus megaterium is inoculated into each self-corresponding liquid nutrient medium.
(4) fermentation culture: the temperature of controlling each liquid nutrient medium is at 30 ℃~37 ℃, and the pH value cultivated 14~24 hours five kinds of desilting bacterium between 6.5~8 in each self-corresponding liquid nutrient medium, obtain five kinds of fermented liquids.
(5) fermented liquid concentrates: five kinds of fermented liquids of step (4) gained are carried out ultrafiltration and concentration respectively; Remove 60% water; And viable bacteria content in detecting every milliliter, obtain five kinds of liquid concentrators of 2.5 times that the desilting bacteria concentration is about desilting bacteria concentration in the fermented liquid.
The mixing of (6) desilting bacterium liquid concentrator: the viable bacteria content proportioning of five kinds of liquid concentrators of actinomycetes, Nitrosomonas europaea, Vickers bacterium nitrobacter, general foster secondary coccus and the bacillus megaterium of step (5) gained being pressed 2:1:1:2:3 is mixed, and obtains mixed bacterium seed liquid.
Cultivation is solidified in (7): under aseptic condition; The porous sodium type active zeolite particle of the mixed bacterium seed liquid of step (6) gained and step (1) gained is even according to the mixed of weight ratio 1/19~1/4, the desilting bacterium is solidified under temperature is 20 ℃~35 ℃, the condition of pH value 6.5~7.5 cultivated 12~18 hours.Desilting bacterium raised growth breeding in this process, they can be the center growth with the zeolite, also can in water, grow with suspended pattern.
(8) drying: will pass through the full dextrin of porous sodium type active zeolite particulate surfaces coated of step (7), zeolite granular and appendix mixed bacterium is above that coated by dextrin fully, dextrin plays protectant effect; Make the zeolite granular of the full dextrin of bag under 30 ℃~50 ℃ temperature condition, carry out forced air drying, moisture content≤10% in said porous sodium type active zeolite particle, the dry completion promptly made said mikrobe desilting agent.
Embodiment 3
present embodiment provides the method for use and the certain applications data of embodiment 2 prepared mikrobe desilting agent, and is as shown in table 1.
The method of use and the application data of table 1 embodiment 2 made mikrobe desilting agent
More than the present invention has been done detailed description; Its purpose is to let the personage that is familiar with this art can understand content of the present invention and implements; Can not limit protection scope of the present invention with this; All equivalences of doing according to spirit of the present invention change or modify, and all should be encompassed in protection scope of the present invention

Claims (3)

1. mikrobe desilting agent; It is characterized in that: said mikrobe desilting agent is a carrier with the porous zeolite particle; With by actinomycetes, Nitrosomonas europaea, Vickers bacterium nitrobacter, general foster secondary coccus and bacillus megaterium by mixed bacterium that viable bacteria content proportioning 1.8~2.2:0.8~1.2:0.8~1.2:1.8~2.2:2.8~3.2 constitute as the desilting bacterium, the preparation method of said mikrobe desilting agent comprises the steps:
(1) activation treatment of zeolite: clinoptilolite is handled with excessive sodium salt solution, made Na +Exchange rate descended dry 2~3 hours at 90 ℃~120 ℃ more than 75% then, again 400 ℃~600 ℃ following heat activation 2~3 hours, obtained porous sodium type active zeolite particle;
(2) individual plant of desilting bacterium is cultivated: comprise that inoculation, fermentation culture and fermented liquid concentrate, and obtain the liquid concentrator of five kinds of desilting bacterium at last;
(3) mixing of desilting bacterium liquid concentrator: the liquid concentrator of step (2) gained actinomycetes, Nitrosomonas europaea, Vickers bacterium nitrobacter, general foster secondary coccus and bacillus megaterium is mixed by the viable bacteria content proportioning of 1.8~2.2:0.8~1.2:0.8~1.2:1.8~2.2:2.8~3.2, obtain mixed bacterium seed liquid;
(4) solidify cultivation: under aseptic condition; The porous sodium type active zeolite particle of step (3) gained mixed bacterium seed liquid and step (1) gained is even according to the mixed of weight ratio 1/19~1/4, be to solidify under 20 ℃~35 ℃, the condition of pH value to cultivate 12~18 hours in temperature then 6.5~7.5;
(5) drying: the porous sodium type active zeolite particulate surfaces coated through step (4) expires dextrin; Zeolite granular and appendix mixed bacterium is above that coated by dextrin fully; And under 30 ℃~50 ℃ temperature condition, carry out drying; Moisture content≤10% in said porous sodium type active zeolite particle, the dry completion obtains said mikrobe desilting agent;
Wherein, the fermentation culture process in the step (2) is: making five kinds of liquid nutrient mediums inoculating is that 30 ℃~37 ℃, pH value are to cultivate 14~24 hours under 6.5~8 the condition in temperature, obtains five kinds of fermented liquids;
Wherein, the fermented liquid concentration process in the step (2) is: said five kinds of fermented liquids are carried out ultrafiltration and concentration respectively, and obtaining the desilting bacteria concentration is five kinds of liquid concentrators of 2.5 times of desilting bacteria concentration in the fermented liquid.
2. mikrobe desilting agent according to claim 1 is characterized in that: said porous zeolite is that size is at 40 orders~250 purpose porous sodium type active zeolites.
3. the preparation method of mikrobe desilting agent according to claim 1 is characterized in that: used liquid nutrient medium, pH value regulator and other apparatus or reagent all pass through high pressure steam sterilization among the said preparation method.
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Effective date of registration: 20191028

Address after: Suzhou City, Jiangsu province 215300 Kunshan economic and Technological Development Zone East Road chapter

Patentee after: Kunshan Huake Membrane and Environmental Technology Co., Ltd.

Address before: Suzhou City, Jiangsu Province, Kunshan City Development Zone, 215300 East Road chapter Huake biological building

Patentee before: Huake Institute of Bio-polymeric Materials, Kunshan Industrial Technology Resear