CN107265659A - A kind of aquaculture microorganism substrate modifier with triple function and preparation method thereof - Google Patents
A kind of aquaculture microorganism substrate modifier with triple function and preparation method thereof Download PDFInfo
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- CN107265659A CN107265659A CN201710499636.9A CN201710499636A CN107265659A CN 107265659 A CN107265659 A CN 107265659A CN 201710499636 A CN201710499636 A CN 201710499636A CN 107265659 A CN107265659 A CN 107265659A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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Abstract
The invention discloses a kind of aquaculture microorganism substrate modifier with triple function and preparation method thereof, remain to keep high viable bacteria content during production and transportation and remain to efficiently play its effect in the low dissolved oxygen condition of pond bottom.The organic substances such as decomposable asymmetric choice net residual bait, excrement, suppress pathogenic microorganism；Organophosphor in water body is converted into Phos, promotes the growth of phytoplankton；Nitrite nitrogen in water body can be converted into nitrogen and depart from water body, water nitrite nitrogen content is reduced；Heavy metal ion can be chelated, heavy metal toxicity is reduced, with bottom, water transfer, removing toxic substances triple function is changed, omnibearing stereo improves water body environment.A kind of aquaculture microorganism substrate modifier with triple function, includes following component according to mass ratio：13 parts of zeolite powder；28 parts of bentonite；0.5 4 parts of diatomite；28 parts of 13 parts of polyglutamic acid pulvis and compound probiotic bacterium powder.
Description
Technical field
The invention belongs to aquaculture water environment based technique for in-situ remediation field.Specifically related to a kind of water with triple function
Production cultivation microorganism substrate modifier.
Background technology
The problem of fast-developing intensive aquatic products industry also brings new while increasing economic efficiency, in high-density breeding
Under pattern, breeding water body self-pollution is very serious, and the research of many scholars shows, in breeding process bait utilization compared with
Low, the organic matter accumulation such as substantial amounts of residual bait, biological metabolite, animals and plants corpse is in culturing pool bottom in breeding process, and these are organic
Thing corrupt can decompose in the presence of anaerobic bacteria and produce a large amount of materials poisonous to aquiculture animal, cause breeding water body
Physicochemical environment and ecological environment severe exacerbation；Pathogenic microorganism species increases makes biological disease be on the rise soon with spread speed.
Aquaculture substrate deteriorates causes heavy losses to culture fishery.The improvement of aquaculture substrate is that aquaculture water quality changes
Good crucial and basis.The substrate modifier used at present mainly includes two major classes：Chemical classes and microbiology class.Wherein chemical classes
Bottom changes including chemosorbent, complexing agent, oxygenation agent, disinfectant, reducing agent, high molecular polymer etc., and its action principle is wadding
Harmful substance in solidifying water body, makes it sink to bottom of pond after being combined with itself.Although harmful substance is reduced in water body, because of flocculation
Harmful substance to bottom of pond is excessive and can not remove, therefore its effect can only alleviate the pollution of water body, it is impossible to fundamentally remove
Pollution.Microbiology class bottom changes main using lactic acid bacteria class or aerobic bacillus category at present.Wherein lactic acid bacteria can not form bud
Spore, resistance is poor, is changed a social system the bottom of at and makees and easily inactivated during preserving, practical application effect is poor.And aerobic gemma bar
Although mushroom strong stress resistance, the low dissolved oxygen environment of single aquaculture pond bottom can suppress the propagation of this quasi-microorganism, Jin Erying
Ring its using effect.And to change product function single at bottom at present, it is impossible to improve aquaculture water quality and bottom environment from solid.
The content of the invention
Supported in view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to which the present invention provides a kind of aquatic products with triple function
Microorganism substrate modifier and preparation method thereof is grown, remains to keep high viable bacteria content and in pond during production and transportation
The low dissolved oxygen condition in bottom remains to efficiently play its effect.The organic substances such as decomposable asymmetric choice net residual bait, excrement, suppress pathogenic microorganism；Will
Organophosphor in water body is converted into Phos, promotes the growth of phytoplankton；Nitrite nitrogen in water body can be converted into
Nitrogen departs from water body, reduces water nitrite nitrogen content；It can chelate heavy metal ion, reduce heavy metal toxicity, with changing
Bottom, water transfer, removing toxic substances triple function, omnibearing stereo improve water body environment.
The purpose of the present invention is realized by following technique effect：A kind of aquaculture microorganism with triple function
Substrate modifier, includes following component according to mass ratio：1-3 parts of zeolite powder；2-8 parts of bentonite；0.5-4 parts of diatomite；Poly- paddy
2-8 parts of 1-3 parts of propylhomoserin pulvis and compound probiotic bacterium powder.
Further, described compound probiotic bacterium powder includes following component according to mass ratio：Clostridium butyricum opportunistic pathogen powder 2-8
Part；2-8 parts of bacillus coagulans；1-4 parts of enterococcus faecalis opportunistic pathogen powder；1-4 parts of bacillus megaterium and denitrifying bacteria 0.5-4
Part.
Further, total viable bacteria content 5 × 10 of the microorganism substrate modifier8-5×109cfu/g
Further, the viable bacteria content of described clostridium butyricum opportunistic pathogen powder is 1 × 1010cfu/g；The viable bacteria of bacillus coagulans
Content is 1 × 1010cfu/g；The viable bacteria content of enterococcus faecalis is 1 × 1010cfu/g；The viable bacteria content of bacillus megaterium is
1×1010cfu/g；The viable bacteria content of denitrifying bacteria is 1 × 1010 cfu/g。
Further, the content of gamma-polyglutamic acid is 80g/kg in described polyglutamic acid pulvis.
The preparation method of aquaculture microorganism substrate modifier with triple function, comprises the following steps：According to weight
Amount proportioning weighs zeolite powder, bentonite, diatomite, polyglutamic acid pulvis, compound probiotic bacterium powder, and above-mentioned raw materials are mixed and stirred
Mix uniform, the aquaculture microorganism substrate modifier with triple function is obtained through disc granulation, sieving, drying, packing.
Further, described compound probiotic bacterium powder is prepared by following technique：Clostridium butyricum opportunistic pathogen powder, condensation gemma bar
Bacterium opportunistic pathogen powder, bacillus megaterium opportunistic pathogen powder are produced by liquid-state fermentation technology, and technical process includes：Strain purification, kind
The techniques such as sub- culture, main fermentation tank culture, using dregs of beans, corn, drum head as culture medium, then add the sucrose of culture medium total amount 1%,
Then potassium dihydrogen phosphate, magnesium sulfate, B family vitamin cultivates 28-36 hours at 25-35 DEG C and obtains bacterium solution.Clostridium butyricum opportunistic pathogen
Powder, bacillus coagulans opportunistic pathogen powder, bacillus megaterium opportunistic pathogen powder are spray-dried to be obtained, enterococcus faecalis and denitrifying bacteria warp
Freeze-drying is obtained, and is weighed by weight ratio after above-mentioned opportunistic pathogen powder is well mixed and is obtained compound probiotic bacterium powder.
Further, polyglutamic acid pulvis is prepared by following techniques：Polyglutamic acid is by the limited public affairs of Jiangsu distant mountain biotechnology
The bacillus natto to ferment of department's production is obtained, with maltose, big soya-bean milk, sodium glutamate, K2HPO4、MgSO4·7H20 is culture
Base, the NaCl of addition 3% prevents to foam with effective, cultivates 4d at 37 DEG C, adjusts pH value, filtering, filtrate is added to 3 times of volumes
Ethanol in, collect precipitation obtain polyglutamic acid pulvis.
The beneficial effects of the present invention are：High-effective microorganism substrate modifier obtained above is spherical of yellow-white
Grain, viable bacteria content is 5 × 108-5×109cfu/g.Suitable for the aquatic product animal raise seedling such as fish shrimp crab shellfish pond and growing pond water quality or bottom
Matter is improved, and especially suitable pond bottom mud is more, the relatively low pond of bottom dissolved oxygen.
The usage and dosage of above-mentioned high-effective microorganism substrate modifier is：Full pond is uniformly shed, and every mu is used 400-500g, often
10-15 days using once.When water quality aging or thicker bottom of pond mud, every mu uses 800-1000g, every to use one within 7-10 days
It is secondary.
Clostridium butyricum is strict anaerobes, and bacillus coagulans and enterococcus faecalis are facultative anaerobic bacteria, these three beneficial bacteriums
Can the amount reproduction growth under low dissolved oxygen condition in pond waters bottom and mud, the residual bait of decomposition breeding water body bottom, excrement
Just, animals and plants corpse, is converted into the nutrient salts needed for phytoplankton growth, promotes the benign cycle of water nutrition material, prevents
The only accumulation of poisonous and harmful substance.In addition, they can produce antibacterial substance in growth course, suppress pond bottom and water body disease
The propagation of pathogenic microorganism, with certain prophylactic effect.
Clostridium butyricum and bacillus coagulans exist in processing and storage with spore form, and strong stress resistance can be grown
Phase keeps bioactivity, overcomes traditional lactic acid bacteria class bottom and changes into the problem of viable count declines fast in processing and transportation.Fourth
Sour clostridium and bacillus coagulans can amount reproduction grows under low dissolved oxygen condition in pond waters bottom and mud, overcome with
Microorganism bottom based on aerobic bacillus changes the problem of can not adapting to pool bottom environment condition.
Denitrifying bacteria is facultative anaerobic bacteria, can be by the nitrate in water body under the anaerobic condition of aquaculture pond bottom
It is that nitrogen overflows water body with Nitrite transformation, the effect with biological denitrificaion reduces the accumulation of nitrite and into water body
Release, fundamentally alleviates water body nitrate pollution problem, prevents aquatic livestock nitrite poisoning.
Bacillus megaterium is called phosphate solubilizing bacteria, organophosphor can be converted into Phos, is that growing for phytoplankton carries
For necessary inorganic P elements, promote the circulation of water systems'phosphorus, promote phytoplankton growth, reduce the usage amount of phosphate fertilizer.
Gamma-polyglutamic acid is a kind of by the polymer of Pidolidone and D-Glu by dehydrating condensation, containing big
Amount amido link and the carboxyl that can dissociate, heavy metal ion have very strong adsorptivity and sequestering, and research shows it to Pb2+、
Cr3 +、Ni2 +、Cu2 +And Mn2 +It is respectively provided with very strong chelation.Using polyglutamic acid can reduce in breeding water body heavy metal from
Sub- content, prevents heavy metal ion from being accumulated in cultivated animals body, improves aquatic products quality.
By above-mentioned several microorganisms and gathered propylhomoserin pulvis with the use of on the one hand can reduce pond bottom residual bait, excrement,
The accumulation of animals and plants corpse, reduction ammonia nitrogen, nitrite, the content of hydrogen sulfide and heavy metal ion, with changing bottom and removing toxic substances
Effect.On the other hand, the invalid phosphorus in water body can be converted into available phosphorus by bacillus megaterium, improve the utilization rate of phosphorus, tool
Play the role of to cultivate algae regulating water quality, water body environment can be effectively improved.
The new and effective aquaculture microorganism substrate modifier of the present invention has following effect：
1st, water bottom environment is optimized：Beneficial bacterium acts on bottom of pond, the rapid ammonia reduced because of water quality deterioration or excess generation of applying fertilizer
The harmful substances such as nitrogen, hydrogen sulfide, nitrite, eliminate that water colour is excessively black and bed mud is smelly, heating, yellow mud water or slime water etc. it is existing
As.
2nd, beneficial algae growth is promoted：It is the nutrients that beneficial algae grows by the organic pollution decomposition and inversion of bottom of pond
Matter, promotes beneficial algae growth, water body is reached that bacterium algae is balanced.The content of bottom of pond reducing substances is reduced, the oxygen of bottom of pond is improved
Change reduction potential, indirectly increase water body dissolved oxygen.
3rd, pathogenetic bacteria breeding is suppressed：Beneficial bacterium produces ablastins in metabolic process, suppresses bottom of pond pathogen and condition
The growth and breeding of pathogenic bacteria, purifies substrate.
4th, heavy metal toxicity is reduced：By heavy metal ion adsorbed and pond bottom, Heavy Metals in Waters content is reduced, is alleviated
The symptom such as floating head, apocleisis caused by heavy metal poisoning.
5th, fine water is kept：Periodically using the generation for effectively suppressing moss and blue-green algae, it is ensured that water quality holding " refreshing, tender,
The excellent condition of fresh, work ".
The present invention is as follows in the points for attention for producing and using：
1st, sunlight is prevented directly to shine in the production and use process.
2nd, it should avoid being used together with chemical classes antibacterial insecticidal materials.
Embodiment
The particular content of the present invention is described in detail with reference to specific embodiment.
In following embodiments, clostridium butyricum opportunistic pathogen powder, bacillus coagulans opportunistic pathogen powder, enterococcus faecalis opportunistic pathogen powder, huge bud
Spore bar opportunistic pathogen powder, denitrifying bacteria opportunistic pathogen powder and polyglutamic acid pulvis are Jiangsu distant mountain Bioisystech Co., Ltd product.
Embodiment 1
Clostridium butyricum bacterium powder, bacillus coagulans bacterium powder, denitrifying bacteria bacterium powder, bacillus megaterium bacterium powder, enterococcus faecalis bacterium
Powder viable bacteria content is 1 × 1010cfu/g。
3 parts of clostridium butyricum opportunistic pathogen powder is weighed by weight；3 parts of bacillus coagulans；1.5 parts of enterococcus faecalis opportunistic pathogen powder；It is huge
1.5 parts of bacillus；Composite bacterium powder is obtained after 1 part of denitrifying bacteria is well mixed.
2 parts of zeolite powder is weighed by weight；3 parts of bentonite；1 part of diatomite；1 part of polyglutamic acid pulvis；Compound probiotic bacterium
3 parts of powder.Above-mentioned raw materials are mixed and stirred for uniformly, to obtain high-effective microorganism substrate through disc granulation, sieving, drying, packing and change
Good dose.
According to every 7 days using once, one meter of depth of water of every mu of water surface uses 800g, and high-effective microorganism substrate modifier is direct
Uniform shed to the fish pond of main foster hybridized prussian carp carries out water quality improvement.
The use of high-effective microorganism substrate modifier before processing ammonia nitrogen is that 0.8mg/L, nitrite are 0.3mg/L, water body is muddy
Turbid, ammonia nitrogen is that 0.1mg/L, nitrite are 0.05mg/ L after processing, and water colour lives, tender, refreshing.
Embodiment 2
Bacterium powder viable bacteria content be the same as Example 1.
4 parts of clostridium butyricum opportunistic pathogen powder is weighed by weight；2 parts of bacillus coagulans；1 part of enterococcus faecalis opportunistic pathogen powder；Huge bud
2 parts of spore bacillus；Composite bacterium powder is obtained after 1 part of denitrifying bacteria is well mixed.
1 part of zeolite powder is weighed by weight；3.5 parts of bentonite；0.5 part of diatomite；1.5 parts of polyglutamic acid pulvis；Compound benefit
Raw 3.5 parts of bacterium bacterium powder.Above-mentioned raw materials are mixed and stirred for uniformly, efficient micro- life to be obtained through disc granulation, sieving, drying, packing
Thing substrate modifier.
According to every 10 days using once, one meter of depth of water of every mu of water surface uses 500g, and high-effective microorganism substrate modifier is straight
Connect uniform shed to the pond of main foster river crab and carry out water quality improvement.
The use of high-effective microorganism substrate modifier before processing ammonia nitrogen is that 0.6mg/L, nitrite are 0.3mg/L, water body is muddy
Turbid, ammonia nitrogen is that 0.2mg/L, nitrite are 0.1mg/ L after processing, and water colour lives, tender, refreshing.
Embodiment 3
Bacterium powder viable bacteria content be the same as Example 1.
5 parts of clostridium butyricum opportunistic pathogen powder is weighed by weight；2.5 parts of bacillus coagulans；1 part of enterococcus faecalis opportunistic pathogen powder；It is huge
1 part of bacillus；Composite bacterium powder is obtained after 0.5 part of denitrifying bacteria is well mixed.
1.5 parts of zeolite powder is weighed by weight；2.5 parts of bentonite；1.5 parts of diatomite；1 part of polyglutamic acid pulvis；Compound benefit
Raw 3.5 parts of bacterium bacterium powder.Above-mentioned raw materials are mixed and stirred for uniformly, efficient micro- life to be obtained through disc granulation, sieving, drying, packing
Thing substrate modifier.
According to every 10 days using once, one meter of depth of water of every mu of water surface uses 1000g, and high-effective microorganism substrate modifier is straight
Connect uniform shed to the pond of main foster Macrobrachium rosenbergii and carry out water quality improvement.
The use of high-effective microorganism substrate modifier before processing ammonia nitrogen is that 0.9mg/L, nitrite are 0.23mg/L, after processing
Ammonia nitrogen is that 0.1mg/L, nitrite are 0.07mg/ L, and water colour lives, tender, refreshing.
Embodiment 4
Bacterium powder viable bacteria content be the same as Example 1.
2.5 parts of clostridium butyricum opportunistic pathogen powder is weighed by weight；3.5 parts of bacillus coagulans；1.5 parts of enterococcus faecalis opportunistic pathogen powder；
1.5 parts of bacillus megaterium；Composite bacterium powder is obtained after 1 part of denitrifying bacteria is well mixed.
1.5 parts of zeolite powder is weighed by weight；2.5 parts of bentonite；1.5 parts of diatomite；1 part of polyglutamic acid pulvis；Compound benefit
Raw 3.5 parts of bacterium bacterium powder.Above-mentioned raw materials are mixed and stirred for uniformly, efficient micro- life to be obtained through disc granulation, sieving, drying, packing
Thing substrate modifier.
According to every 8 days using once, one meter of depth of water of every mu of water surface uses 1000g, and high-effective microorganism substrate modifier is straight
Connect uniform shed to the pond of Penaeus Vannmei and carry out water quality improvement.
The use of high-effective microorganism substrate modifier before processing ammonia nitrogen is that 0.85mg/L, nitrite are 0.35mg/L, water body
Ammonia nitrogen is that 0.15mg/L, nitrite are 0.09mg/ L after muddiness, processing, and water colour lives, tender, refreshing.
Embodiment 5
Bacterium powder viable bacteria content be the same as Example 1.
3.5 parts of clostridium butyricum opportunistic pathogen powder is weighed by weight；2.5 parts of bacillus coagulans；1.5 parts of enterococcus faecalis opportunistic pathogen powder；
1 part of bacillus megaterium；Composite bacterium powder is obtained after 2 parts of denitrifying bacteria is well mixed.
3 parts of zeolite powder is weighed by weight；2.5 parts of bentonite；0.5 part of diatomite；1.5 parts of polyglutamic acid pulvis；Compound benefit
Raw 2.5 parts of bacterium bacterium powder.Above-mentioned raw materials are mixed and stirred for uniformly, efficient micro- life to be obtained through disc granulation, sieving, drying, packing
Thing substrate modifier.
According to every 15 days using once, one meter of depth of water of every mu of water surface uses 1000g, and high-effective microorganism substrate modifier is straight
Connect uniform shed to the pond of cultivation cray and carry out water quality improvement.
The use of high-effective microorganism substrate modifier before processing ammonia nitrogen is that 0.6mg/L, nitrite are 0.15mg/L, water body is muddy
Turbid, ammonia nitrogen is that 0.06mg/L, nitrite are 0.02mg/ L after processing, and water colour lives, tender, refreshing.
Above example shows that the present invention has a better role to all kinds of breeding water bodies tool of water quality deterioration.
Claims (8)
1. a kind of aquaculture microorganism substrate modifier with triple function, it is characterised in that：Include such as according to mass ratio
Lower component：1-3 parts of zeolite powder；2-8 parts of bentonite；0.5-4 parts of diatomite；1-3 parts of polyglutamic acid pulvis and compound probiotic bacterium
2-8 parts of powder.
2. according to the aquaculture microorganism substrate modifier with triple function described in claim 1, it is characterised in that：Institute
The compound probiotic bacterium powder stated includes following component according to mass ratio：2-8 parts of clostridium butyricum opportunistic pathogen powder；Bacillus coagulans 2-8
Part；1-4 parts of enterococcus faecalis opportunistic pathogen powder；0.5-4 parts of 1-4 parts of bacillus megaterium and denitrifying bacteria.
3. the aquaculture microorganism substrate modifier according to claim 1 with triple function, it is characterised in that：Institute
State total viable bacteria content 5 × 10 of microorganism substrate modifier8-5×109cfu/g。
4. the aquaculture microorganism substrate modifier according to claim 2 with triple function, it is characterised in that：Institute
The viable bacteria content for the clostridium butyricum opportunistic pathogen powder stated is 1 × 1010cfu/g；The viable bacteria content of bacillus coagulans is 1 × 1010cfu/
g；The viable bacteria content of enterococcus faecalis is 1 × 1010cfu/g；The viable bacteria content of bacillus megaterium is 1 × 1010cfu/g；Denitrification
The viable bacteria content of bacterium is 1 × 1010cfu/g。
5. the aquaculture microorganism substrate modifier according to claim 1 with triple function, it is characterised in that：Institute
The content of gamma-polyglutamic acid is 80g/kg in the polyglutamic acid pulvis stated.
6. according to the preparation method of the aquaculture microorganism substrate modifier with triple function described in claim 2, its
It is characterised by：Comprise the following steps：Weigh zeolite powder according to weight proportion, it is bentonite, diatomite, polyglutamic acid pulvis, compound
Probiotics bacterium powder, above-mentioned raw materials are mixed and stirred for uniformly, to obtain with triple function through disc granulation, sieving, drying, packing
Aquaculture microorganism substrate modifier.
7. according to the preparation method of the aquaculture microorganism substrate modifier with triple function described in claim 6, its
It is characterised by：Described compound probiotic bacterium powder is prepared by following technique：Clostridium butyricum opportunistic pathogen powder, bacillus coagulans opportunistic pathogen
Powder, bacillus megaterium opportunistic pathogen powder are produced by liquid-state fermentation technology, and technical process includes：Strain purification, seed culture,
The techniques such as main fermentation tank culture, using dregs of beans, corn, drum head as culture medium, then add the sucrose of culture medium total amount 1%, di(2-ethylhexyl)phosphate
Then hydrogen potassium, magnesium sulfate, B family vitamin cultivates 28-36 hours at 25-35 DEG C and obtains bacterium solution.It is clostridium butyricum opportunistic pathogen powder, solidifying
The spray-dried acquisition of bacillus opportunistic pathogen powder, bacillus megaterium opportunistic pathogen powder is tied, enterococcus faecalis and denitrifying bacteria are chilled
Dry and obtain, weigh by weight ratio after above-mentioned opportunistic pathogen powder is well mixed and obtain compound probiotic bacterium powder.
8. according to the preparation method of the aquaculture microorganism substrate modifier with triple function described in claim 6, its
It is characterised by：Polyglutamic acid pulvis is prepared by following techniques：Polyglutamic acid is produced by Jiangsu distant mountain Bioisystech Co., Ltd
Bacillus natto to ferment is obtained, with maltose, big soya-bean milk, sodium glutamate, K2HPO4、MgSO4·7H20 is culture medium, addition
3% NaCl is prevented to foam with effective, and 4d is cultivated at 37 DEG C, adjusts pH value, and filtering, filtrate is added in the ethanol of 3 times of volumes,
Collect precipitation and obtain polyglutamic acid pulvis.
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