CN103496791B - A kind of production method of compound microorganism preparation for aquatic product - Google Patents
A kind of production method of compound microorganism preparation for aquatic product Download PDFInfo
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- CN103496791B CN103496791B CN201310449207.2A CN201310449207A CN103496791B CN 103496791 B CN103496791 B CN 103496791B CN 201310449207 A CN201310449207 A CN 201310449207A CN 103496791 B CN103496791 B CN 103496791B
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Abstract
The object of the present invention is to provide a kind of production methods of compound microorganism preparation for aquatic product, by the way that Bacillus cercus, bacillus licheniformis, thermophilic salt pseudomonad, lactobacillus acidophilus, red city Rhodococcus sp, Rhodopseudomonas palustris, manure enterococcin strain are carried out high density liquid fermentation respectively, obtain hyperconcetration bacterium powder, then it is proportionally prepared, obtains complex microorganism preparations.The production method process is simple, is adapted to most of existing zymotechniques and equipment, is conducive to large-scale promotion application.
Description
Technical field
The present invention relates to technical field of microbe application, and in particular to a kind of producer of compound microorganism preparation for aquatic product
Method.
Background technique
Usually in its natural state, the indigenous bacterium in water body is able to maintain that the normal substance of water body follows by self purification
Ring.But with the development of high-density aquiculture, the organic loading in breeding water body has been well beyond oneself of original flora
Net energy power.The organic matter not being used effectively becomes the largest source of pollution in water body, consumes a large amount of dissolved oxygen, causes oxygen in water body
Debt, and then inhibit the complete oxidation of organic matter, form a large amount of incomplete oxidation intermediate, and these incomplete oxidation products,
It is often again that maximum noxious material is endangered in aquaculture, such as ammonia nitrogen, nitrite and hydrogen sulfide cause cultivated animals
Chronic and acute poisoning, serious to cause large-scale dead fish, economic loss is heavy.
In recent years, microorganism formulation started that for purifying water body, the feedings of cultivated animals should be improved in cultivation field extensively
Amount, the generation for reducing disease etc..The beneficial microbe launched in water body is bred by raised growth, is secreted abundant extracellular
Enzyme effectively decomposes organic matter, and microorganism can utilize ammonia nitrogen, energy metabolism nitrite can oxygen during growth metabolism
Change hydrogen sulfide etc., to realize the purpose of degradation noxious material, the culture efficiency that purifies water, improves.
The microorganism formulation of currently used purifying water body mostly uses single bacterial strain or two or three kind of bacterial strain compound action, preparation
In viable count it is relatively low, cannot comprehensively, effectively improve the quality of breeding water body, therefore, the task of top priority at present is active development
Novel complex micro organism fungicide, improves aquiculture status comprehensively.
Summary of the invention
The present invention is to solve prior art problem, provides a kind of complex microorganism preparations and its life for capableing of purifying water body
Production method.Said preparation passes through the collaboration facilitation of multiple-microorganism, has significant water transfer color, drops ammonia nitrogen, nitrite, mentions
The high grade of transparency promotes the functions such as cultivated animals growth.
Applicant had found by long-term screening study, Bacillus cercus, bacillus licheniformis, thermophilic salt pseudomonad,
Synergistic effect can be generated between lactobacillus acidophilus, red city Rhodococcus sp, Rhodopseudomonas palustris and enterococcus faecium, effectively degradation water body
In ammonia nitrogen, nitrite, improve water quality, to facilitate the present invention.
One aspect of the present invention provides a kind of complex microorganism preparations, by Bacillus cercus (Bacillus cereus),
Bacillus licheniformis (Bacillus licheniformis), thermophilic salt pseudomonad (Halomonas sulfidaeris), acidophilus
Lactobacillus (Lactobacillus acidophilus), red city Rhodococcus sp (Rhodococcus erythropolis), marsh it is red
Pseudomonad (Rhodopseudomonas palustris) and enterococcus faecium (Enterococcus faecium) composition.
The preferred Bacillus cercus CGMCC1.10163 of component, bacillus licheniformis in above-mentioned complex microorganism preparations
CGMCC1.10257, thermophilic salt pseudomonad CCTCC M 2013070, lactobacillus acidophilus CGMCC1.3915, red city Rhodococcus sp
CGMCC1.3613, Rhodopseudomonas palustris CGMCC1.2352 and enterococcus faecium CGMCC1.2334.
Each component and its content are respectively as follows: 20-50 parts of Bacillus cercus, lichens gemma in above-mentioned complex microorganism preparations
10-30 parts of bacillus, 5-15 parts of thermophilic salt pseudomonad, 1-5 parts of lactobacillus acidophilus, 1-5 parts of red city Rhodococcus sp, Rhodopseudomonas palustris
10-15 parts, 10-30 parts of enterococcus faecium.
The content of each component is respectively preferably in above-mentioned complex microorganism preparations: 40 parts of Bacillus cercus, lichens gemma
25 parts of bacillus, 10 parts of thermophilic salt pseudomonad, 3 parts of lactobacillus acidophilus, 3 parts of red city Rhodococcus sp, 12 parts of Rhodopseudomonas palustris, dung intestines
22 parts of coccus.
One aspect of the present invention provides application of the above-mentioned complex microorganism preparations in purification of water quality.
The dosage of above-mentioned complex microorganism preparations is 20-40 grams/acre of rice, every other week using primary.
Another aspect of the present invention provides the production method of above-mentioned complex microorganism preparations, includes the following steps:
1) by above-mentioned Bacillus cercus, bacillus licheniformis, thermophilic salt pseudomonad, lactobacillus acidophilus, red city Rhodococcus sp,
High density liquid fermentation is respectively adopted in Rhodopseudomonas palustris, manure enterococcin strain, by zymotechnique post-processing technology, is made
Spore content is up to 1011-1012The hyperconcetration bacterium powder of CFU/g；
2) by hyperconcetration bacterium powder according to 20-50 parts of Bacillus cercus, 10-30 parts of bacillus licheniformis, thermophilic salt vacation unit cell
5-15 parts of bacterium, 1-5 parts of lactobacillus acidophilus, 1-5 parts of red city Rhodococcus sp, 10-15 parts of Rhodopseudomonas palustris, enterococcus faecium 10-30
Part is prepared；
3) complex microorganism preparations obtained are packed using water-soluble material.
Beneficial effect
Viable count is up to 8 × 10 in complex microorganism preparations of the invention11CFU/g can be widely applied to fresh water, seawater is supported
Grow waters.Ammonia nitrogen, the nitrite that can be effectively degraded in water body by the synergistic effect between bacterial strain, inhibit the generation of wawter bloom, from
And purifying aquatic water, improve the growing environment of cultivated animals, improves production performance.Production method provided by the invention is simple,
It is adapted to most of existing zymotechniques and equipment, is conducive to large-scale promotion application.
Specific embodiment
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.In addition, it should also be understood that, after having read the contents of the present invention, those skilled in the art can be with
Various changes or modification are made to the present invention, such equivalent forms equally fall into model defined by the application the appended claims
It encloses.
Various reagents and equipment of the present invention, can be selected from it is commercially available any one；The present invention relates to wax-like in bacterial strain
Bacillus is purchased from China General Microbiological preservation administrative center, deposit number 1.10163；Bacillus licheniformis is general purchased from China
Logical microbial preservation administrative center, deposit number 1.10257；Thermophilic salt pseudomonad is that Qingdao Weilan Biology Group Co., Ltd. is special
Sharp bacterial strain, deposit number CCTCC M 2013070；Lactobacillus acidophilus is purchased from China General Microbiological preservation administrative center, preservation
Number 1.3915；Red city Rhodococcus sp is purchased from China General Microbiological preservation administrative center, deposit number 1.3613；Marsh is red false single
Born of the same parents bacterium is purchased from China General Microbiological preservation administrative center, deposit number 1.2352；Enterococcus faecium is purchased from China General Microbiological
Preservation administrative center, deposit number 1.2334.
Embodiment 1
A kind of complex microorganism preparations, by Bacillus cercus, bacillus licheniformis, thermophilic salt pseudomonad, acidophilus cream bar
Bacterium, red city Rhodococcus sp, Rhodopseudomonas palustris, enterococcus faecium composition, each component and its content are respectively as follows: Bacillus cercus 20
Part, 30 parts of bacillus licheniformis, 15 parts of thermophilic salt pseudomonad, 5 parts of lactobacillus acidophilus, 5 parts of red city Rhodococcus sp, the red false unit cell in marsh
10 parts of bacterium, 30 parts of enterococcus faecium.
The production method of above-mentioned complex microorganism preparations, includes the following steps:
1) by above-mentioned Bacillus cercus, bacillus licheniformis, thermophilic salt pseudomonad, lactobacillus acidophilus, red city Rhodococcus sp,
High density liquid fermentation is respectively adopted in Rhodopseudomonas palustris, manure enterococcin strain, by zymotechnique post-processing technology, is made
Spore content is up to 1011-1012The hyperconcetration bacterium powder of CFU/g；
2) by hyperconcetration bacterium powder according to 20 parts of Bacillus cercus, 30 parts of bacillus licheniformis, 15 parts of thermophilic salt pseudomonad,
5 parts of lactobacillus acidophilus, 5 parts of red city Rhodococcus sp, 10 parts of Rhodopseudomonas palustris, 30 parts of enterococcus faecium prepared；
3) complex microorganism preparations obtained are packed using water-soluble material.
Embodiment 2
A kind of complex microorganism preparations, by Bacillus cercus, bacillus licheniformis, thermophilic salt pseudomonad, acidophilus cream bar
Bacterium, red city Rhodococcus sp, Rhodopseudomonas palustris, enterococcus faecium composition, each component and its content are respectively as follows: Bacillus cercus 50
Part, 10 parts of bacillus licheniformis, 5 parts of thermophilic salt pseudomonad, 1 part of lactobacillus acidophilus, 1 part of red city Rhodococcus sp, the red false unit cell in marsh
15 parts of bacterium, 10 parts of enterococcus faecium.
The production method of above-mentioned complex microorganism preparations, includes the following steps:
1) by above-mentioned Bacillus cercus, bacillus licheniformis, thermophilic salt pseudomonad, lactobacillus acidophilus, red city Rhodococcus sp,
High density liquid fermentation is respectively adopted in Rhodopseudomonas palustris, manure enterococcin strain, by zymotechnique post-processing technology, is made
Spore content is up to 1011-1012The hyperconcetration bacterium powder of CFU/g；
2) by hyperconcetration bacterium powder according to 50 parts of Bacillus cercus, 10 parts of bacillus licheniformis, 5 parts of thermophilic salt pseudomonad,
1 part of lactobacillus acidophilus, 1 part of red city Rhodococcus sp, 15 parts of Rhodopseudomonas palustris, 10 parts of enterococcus faecium prepared；
3) complex microorganism preparations obtained are packed using water-soluble material.
Embodiment 3
A kind of complex microorganism preparations, by Bacillus cercus, bacillus licheniformis, thermophilic salt pseudomonad, acidophilus cream bar
Bacterium, red city Rhodococcus sp, Rhodopseudomonas palustris, enterococcus faecium composition, each component and its content are respectively as follows: Bacillus cercus 40
Part, 25 parts of bacillus licheniformis, 10 parts of thermophilic salt pseudomonad, 3 parts of lactobacillus acidophilus, 3 parts of red city Rhodococcus sp, the red false unit cell in marsh
12 parts of bacterium, 22 parts of enterococcus faecium.
The production method of above-mentioned complex microorganism preparations, includes the following steps:
1) by above-mentioned Bacillus cercus, bacillus licheniformis, thermophilic salt pseudomonad, lactobacillus acidophilus, red city Rhodococcus sp,
High density liquid fermentation is respectively adopted in Rhodopseudomonas palustris, manure enterococcin strain, by zymotechnique post-processing technology, is made
Spore content is up to 1011-1012The hyperconcetration bacterium powder of CFU/g；
2) by hyperconcetration bacterium powder according to 40 parts of Bacillus cercus, 25 parts of bacillus licheniformis, 10 parts of thermophilic salt pseudomonad,
3 parts of lactobacillus acidophilus, 3 parts of red city Rhodococcus sp, 12 parts of Rhodopseudomonas palustris, 22 parts of enterococcus faecium prepared；
3) complex microorganism preparations obtained are packed using water-soluble material.
Embodiment 4
Complex microorganism preparations made from above-described embodiment 1-3 are subjected to count plate, experimental result is as shown in table 1:
Viable count compares in 1 complex microorganism preparations of table
| Sample | Viable count |
| Embodiment 1 | 5×1011CFU/g |
| Embodiment 2 | 2×1011CFU/g |
| Embodiment 3 | 8×1011CFU/g |
Application of 5 complex microorganism preparations of embodiment in lefteye flounder Cultivated water
Selecting the relatively similar lefteye flounder aquaculture pond of two water body environment features, water surface area is 1 mu, about 1.5 meters of the depth of water,
It is divided into control group and test group.Control group is without any processing, and test group presses 25 grams/acre of rice dosages for the above-mentioned implementation of the present invention
The complex microorganism preparations that example is prepared directly are shed near aerator, are started aerator 1 hour, are filled microorganism formulation
It scatter, function and effect are more abundant.Every other day the same period takes control group respectively and test group water sample carries out water quality detection, even
Continuous detection 7 days, as a result as described in Table 2.
2 lefteye flounder Cultivated water water quality detection result of table
"-" expression is not detected.
From table 2 it can be seen that complex microorganism preparations of the invention can significantly improve the transparency of Cultivated water water body, drop
The harmful substances such as ammonia nitrogen, nitrite and hydrogen sulfide are solved, environmental pollution is mitigated, provide the growing environment of health for cultivated animals,
Improve the production performance of cultivated animals.
Claims (3)
1. a kind of production method of complex microorganism preparations, includes the following steps:
1) by Bacillus cercus CGMCC1.10163, bacillus licheniformis CGMCC1.10257, thermophilic salt pseudomonad CCTCC M
2013070, lactobacillus acidophilus CGMCC1.3915, red city Rhodococcus sp CGMCC1.3613, Rhodopseudomonas palustris
High density liquid fermentation is respectively adopted in CGMCC1.2352, enterococcus faecium CGMCC1.2334 bacterial strain, is post-processed by zymotechnique
Technology is made spore content and is up to 1011-1012The hyperconcetration bacterium powder of CFU/g；
2) by hyperconcetration bacterium powder according to 20-50 parts of Bacillus cercus, 10-30 parts of bacillus licheniformis, thermophilic salt pseudomonad 5-
15 parts, 1-5 parts of lactobacillus acidophilus, 1-5 parts of red city Rhodococcus sp, 10-15 parts of Rhodopseudomonas palustris, 10-30 parts of enterococcus faecium into
Row is prepared；
3) complex microorganism preparations obtained are packed using water-soluble material.
2. production method as described in claim 1, which is characterized in that step 2) is by hyperconcetration bacterium powder according to Bacillus cercus 40
Part, 25 parts of bacillus licheniformis, 10 parts of thermophilic salt pseudomonad, 3 parts of lactobacillus acidophilus, 3 parts of red city Rhodococcus sp, the red false unit cell in marsh
12 parts of bacterium, 22 parts of enterococcus faecium prepared.
3. the complex microorganism preparations that production method as claimed in claim 1 or 2 is prepared.
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| CN104186960B (en) * | 2014-07-02 | 2017-01-11 | 广州大学 | Epinephelus coioides feed additive and application method thereof |
| CN104611279B (en) * | 2015-03-02 | 2017-06-09 | 中蓝连海设计研究院 | A kind of red city Rhodococcus sp LH N13 and its microbial bacterial agent and purposes |
| CN105918620A (en) * | 2016-05-06 | 2016-09-07 | 江苏东南生物科技有限公司 | Microbial agent for aquatic product feeds |
| US11439172B2 (en) | 2017-07-03 | 2022-09-13 | Oakbio, Inc. | Microbial biomass based feed products |
| CN108179112B (en) * | 2018-01-23 | 2021-05-18 | 杭州富阳佳畅机械有限公司 | Method for producing hydrogen by chlorella pyrenoidosa combined bacteria |
| CN108179113B (en) * | 2018-01-23 | 2021-05-18 | 杭州富阳佳畅机械有限公司 | Hydrogen-producing composite biological agent |
| CN109497343A (en) * | 2018-11-09 | 2019-03-22 | 南宁学院 | A kind of Pangasius sutchi disease-resistant growth-promoting preparation |
| CN111909865A (en) * | 2020-07-06 | 2020-11-10 | 薛松晓 | Compound microbial preparation for air deodorization and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030086477A (en) * | 2003-09-24 | 2003-11-10 | 최성호 | Microbial Agent for Waste Water Treatment |
| CN1528681A (en) * | 2003-09-28 | 2004-09-15 | 无锡中顺生物技术有限公司 | Live bacterial microorganism water modifier equipped with activating culture medium and manufacturing process thereof |
| CN1563342A (en) * | 2004-02-10 | 2005-01-12 | 凌亮 | Microbe preparation in use for treating high difficult wastewater and preparation method |
| CN102219310A (en) * | 2011-04-11 | 2011-10-19 | 武汉中傲天乐生物科技有限公司 | Environment-friendly composite microbial preparation, preparation thereof, and application thereof to aquaculture |
| CN103146619A (en) * | 2013-03-21 | 2013-06-12 | 青岛蔚蓝天成生物科技有限公司 | Halomonas sulfidaeris and application thereof |
-
2013
- 2013-09-29 CN CN201310449207.2A patent/CN103496791B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030086477A (en) * | 2003-09-24 | 2003-11-10 | 최성호 | Microbial Agent for Waste Water Treatment |
| CN1528681A (en) * | 2003-09-28 | 2004-09-15 | 无锡中顺生物技术有限公司 | Live bacterial microorganism water modifier equipped with activating culture medium and manufacturing process thereof |
| CN1563342A (en) * | 2004-02-10 | 2005-01-12 | 凌亮 | Microbe preparation in use for treating high difficult wastewater and preparation method |
| CN102219310A (en) * | 2011-04-11 | 2011-10-19 | 武汉中傲天乐生物科技有限公司 | Environment-friendly composite microbial preparation, preparation thereof, and application thereof to aquaculture |
| CN103146619A (en) * | 2013-03-21 | 2013-06-12 | 青岛蔚蓝天成生物科技有限公司 | Halomonas sulfidaeris and application thereof |
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