CN111909865A - Compound microbial preparation for air deodorization and application thereof - Google Patents

Compound microbial preparation for air deodorization and application thereof Download PDF

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CN111909865A
CN111909865A CN202010641982.8A CN202010641982A CN111909865A CN 111909865 A CN111909865 A CN 111909865A CN 202010641982 A CN202010641982 A CN 202010641982A CN 111909865 A CN111909865 A CN 111909865A
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microbial preparation
lactobacillus acidophilus
streptococcus thermophilus
pseudomonas putida
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薛松晓
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34—Chemical or biological purification of waste gases
    • B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
    • B01D53/84—Biological processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34—Chemical or biological purification of waste gases
    • B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
    • B01D53/86—Catalytic processes
    • B01D53/8603—Removing sulfur compounds
    • B01D53/8612—Hydrogen sulfide
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34—Chemical or biological purification of waste gases
    • B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
    • B01D53/86—Catalytic processes
    • B01D53/8621—Removing nitrogen compounds
    • B01D53/8634—Ammonia
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2251/00—Reactants
    • B01D2251/95—Specific microorganisms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2257/00—Components to be removed
    • B01D2257/90—Odorous compounds not provided for in groups B01D2257/00 - B01D2257/708
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters

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  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental & Geological Engineering (AREA)
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  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Molecular Biology (AREA)
  • Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a microbial preparation for air deodorization and application thereof, which can effectively inhibit the reproduction of germs, reduce the concentration of ammonia gas, hydrogen sulfide and other odors in the air of places such as toilets, farms, garbage farms and the like and inhibit odor-producing elements through the synergistic action among lactobacillus acidophilus, streptococcus thermophilus, pseudomonas putida, rhodopseudomonas palustris, bacillus pumilus and enterococcus faecium, thereby obviously improving the air quality and the living environment of people. The production method provided by the invention is simple, is suitable for most of the existing fermentation processes and equipment, and is beneficial to large-scale popularization and application.

Description

Compound microbial preparation for air deodorization and application thereof
Technical Field
The invention relates to the technical field of microorganisms, in particular to a microbial preparation for air deodorization and application thereof.
Background
Malodorous pollution is one of the seven recognized environmental hazards in the world. Common malodorous pollutants comprise ammonia, trimethylamine, hydrogen sulfide, methyl mercaptan, dimethyl disulfide, carbon disulfide, styrene and the like, mainly come from industrial and agricultural production departments and life of people, such as feces odor, fish odor, putrefactive odor, rotten fruit odor, potherb odor and the like generated by microbial fermentation in agriculture and animal husbandry production and processing. Sulfides, hydrocarbons, benzenes, phenols, and ammonias, as well as tar, ammonia, and various organic solvents, which are generated during petrochemical production, and offensive odors of urban public facilities. High concentrations of malodorous contaminants can be a direct health hazard to humans.
At present, the method for removing the malodors can be divided into two main aspects of chemical deodorization and biological deodorization, the chemical deodorization principle is to oxidize and degrade malodorous components by using exogenous strong oxides so as to eliminate the malodors, although the method has strong pertinence and quick response, the application range is small, when complex malodorous gas components are encountered, the deodorization efficiency is invalid, and the residual chemical substances after the chemical deodorant is used often cause secondary pollution. The biological deodorization principle is to remove the malodor by directly degrading the malodor by exogenous functional strains or biological enzymes or inhibiting the malodor-producing microorganisms. The biological deodorization has the characteristics of lasting elimination of the odor, no secondary pollution, convenient use, low cost and the like, becomes a research hotspot for controlling the odor, and finds effective and green biological deodorization products and methods, thereby becoming the urgent need of the society at present.
Disclosure of Invention
The invention provides a compound microbial preparation which can remove malodorous pollutants in air, inhibit the propagation of pathogenic bacteria and further purify the air to solve the problems in the prior art. The preparation can effectively inhibit odor-producing elements and degrade odor pollutants by the synergistic promotion effect of multiple microorganisms, thereby reducing the concentration of harmful gases and reducing the concentration of odor.
The applicant discovers through long-term screening research that the synergistic effect can be generated among lactobacillus acidophilus, streptococcus thermophilus, pseudomonas putida, rhodopseudomonas palustris, bacillus pumilus and enterococcus faecium, the concentration of ammonia gas, hydrogen sulfide and other odors in the air can be effectively reduced, and saprophytic bacteria can be inhibited from propagating, so that the invention is promoted.
The invention provides a deodorizing compound microbial preparation which comprises lactobacillus acidophilus, streptococcus thermophilus, pseudomonas putida, rhodopseudomonas palustris, bacillus pumilus and enterococcus faecium.
The above deodorizing microorganism preparation preferably contains Lactobacillus acidophilus, Streptococcus thermophilus, Pseudomonas putida, Rhodopseudomonas palustris, Bacillus pumilus and enterococcus faecium.
The deodorizing microbial preparation comprises the following components in percentage by weight: 40-60 parts of lactobacillus acidophilus, 10-35 parts of streptococcus thermophilus, 45-60 parts of pseudomonas putida, 5-15 parts of rhodopseudomonas palustris, 10-20 parts of bacillus pumilus and 10-40 parts of enterococcus faecium.
The content of each component in the deodorizing microorganism preparation is preferably 45 parts of lactobacillus acidophilus, 25 parts of streptococcus thermophilus, 51 parts of pseudomonas putida, 10 parts of rhodopseudomonas palustris, 15 parts of bacillus pumilus and 35 parts of enterococcus faecium.
On the other hand, the preparation method of the deodorizing microbial preparation comprises the following steps:
1) respectively fermenting lactobacillus acidophilus, streptococcus thermophilus, pseudomonas putida, rhodopseudomonas palustris, bacillus pumilus and enterococcus faecium by adopting high-density liquid to prepare bacterial liquid with 200 hundred million CFU viable bacteria;
2) preparing a compound microbial preparation from the bacterial liquid according to 40-60 parts of lactobacillus acidophilus, 10-35 parts of streptococcus thermophilus, 45-60 parts of pseudomonas putida, 5-15 parts of rhodopseudomonas palustris, 10-20 parts of bacillus pumilus and 10-40 parts of enterococcus faecium, adding apple juice according to the volume ratio of 6-10%, and uniformly mixing to obtain the liquid deodorant microbial preparation.
The invention also provides the application of the deodorizing microorganism preparation in air purification.
The liquid deodorant microbial preparation is sprayed to places with high concentration of malodorous substances, such as toilets, farms, garbage transfer station garbage dumps and the like, 1 time per week, and the dosage of each time is 250 ml/ton.
The composite microbial preparation provided by the invention can effectively reduce the concentration of ammonia gas, hydrogen sulfide and other odor in the air of places such as toilets, farms, garbage dumps and the like and inhibit odor-producing elements through the synergistic effect among lactobacillus acidophilus, streptococcus thermophilus, pseudomonas putida, rhodopseudomonas palustris, bacillus pumilus and enterococcus faecium, thereby obviously improving the air quality and the living environment of people. The production method provided by the invention is simple, is suitable for most of the existing fermentation processes and equipment, and is beneficial to large-scale popularization and application.
[ detailed description ] embodiments
The various reagents and devices related to the invention can be selected from any one of the reagents and devices which are commercially available; the invention relates to a strain in which lactobacillus acidophilus is purchased from China center for Industrial microbiological Collection center (CICC) with a preservation number: 6075; the streptococcus thermophilus is purchased from China general microbiological preservation management center with the preservation number of CGMCC1.3996, the pseudomonas putida is purchased from China general microbiological preservation management center with the preservation number of CGMCC1.2309, the rhodopseudomonas palustris is a patent strain of Suaeda glauca biological group, the preservation number of CGMCC1.2349, the bacillus pumilus is purchased from China general microbiological preservation management center with the preservation number of CGMCC1.8112, and the enterococcus faecium is purchased from China general microbiological preservation management center with the preservation number of CGMCC 1.2136.
Example 1
A deodorizing microbial preparation comprises 40 parts of lactobacillus acidophilus, 25 parts of streptococcus thermophilus, 450 parts of pseudomonas putida, 10 parts of rhodopseudomonas palustris, 15 parts of bacillus pumilus and 20 parts of enterococcus faecium.
The preparation method of the deodorizing microbial preparation comprises the following steps:
1) respectively fermenting lactobacillus acidophilus, streptococcus thermophilus, pseudomonas putida, rhodopseudomonas palustris, bacillus pumilus and enterococcus faecium by adopting high-density liquid, and preparing the fermentation liquid into bacterial liquid with the viable bacteria number of 200 CFU;
2) the liquid is prepared into a composite microbial preparation according to 40 parts of lactobacillus acidophilus, 25 parts of streptococcus thermophilus, 450 parts of pseudomonas putida, 10 parts of rhodopseudomonas palustris, 15 parts of bacillus pumilus and 20 parts of enterococcus faecium, apple juice is added according to the volume ratio of 6%, and the liquid deodorant microbial preparation is obtained by uniformly mixing.
Example 2
A deodorizing microbial preparation comprises 48 parts of lactobacillus acidophilus, 20 parts of streptococcus thermophilus, 55 parts of pseudomonas putida, 8 parts of rhodopseudomonas palustris, 11 parts of bacillus pumilus and 33 parts of enterococcus faecium.
The preparation method of the deodorizing microbial preparation comprises the following steps:
1) respectively fermenting lactobacillus acidophilus, streptococcus thermophilus, pseudomonas putida, rhodopseudomonas palustris, bacillus pumilus and enterococcus faecium by adopting high-density liquid, and preparing the fermentation liquid into bacterial liquid with the viable count of 200 CFU;
2) preparing a compound microbial preparation from the bacterial liquid according to 48 parts of lactobacillus acidophilus, 20 parts of streptococcus thermophilus, 55 parts of pseudomonas putida, 8 parts of rhodopseudomonas palustris, 11 parts of bacillus pumilus and 33 parts of enterococcus faecium, adding apple juice according to the volume ratio of 8%, and uniformly mixing to obtain the liquid deodorant microbial preparation.
Example 3
A deodorizing microbial preparation comprises 48 parts of lactobacillus acidophilus, 20 parts of streptococcus thermophilus, 55 parts of pseudomonas putida, 8 parts of rhodopseudomonas palustris, 11 parts of bacillus pumilus and 33 parts of enterococcus faecium.
The preparation method of the deodorizing microbial preparation comprises the following steps:
1) respectively fermenting lactobacillus acidophilus, streptococcus thermophilus, pseudomonas putida, rhodopseudomonas palustris, bacillus pumilus and enterococcus faecium by adopting high-density liquid, and preparing the fermentation liquid into bacterial liquid with the viable count of 200 CFU;
2) preparing a compound microbial preparation from the bacterial liquid according to 44 parts of lactobacillus acidophilus, 35 parts of streptococcus thermophilus, 47 parts of pseudomonas putida, 9 parts of rhodopseudomonas palustris, 12 parts of bacillus pumilus and 25 parts of enterococcus faecium, adding apple juice according to the volume ratio of 9%, and uniformly mixing to obtain the liquid deodorant microbial preparation.
EXAMPLE 4 use of deodorizing microbial preparation
Adding 250 ml of the deodorizing microbial preparation of the invention into a fermentation tank filled with 1000 kg of domestic garbage, uniformly mixing the deodorizing microbial preparation with the garbage, and covering the surface layer of the fermentation tank with a plastic film; at the same time, the control without adding the microbial inoculum is carried out, three times of experiments are set for each group, and H is measured after 7 days2S、SO2And VOCSThe concentrations, the results of the measurements are shown in the following table:
Figure 724972DEST_PATH_IMAGE002

Claims (5)

1. a composite microbial preparation for air deodorization is characterized by comprising lactobacillus acidophilus, streptococcus thermophilus, pseudomonas putida, rhodopseudomonas palustris, bacillus pumilus and enterococcus faecium.
2. The complex microbial preparation for air deodorization as set forth in claim 1, wherein: the contents of each component are 40-60 parts of lactobacillus acidophilus, 10-35 parts of streptococcus thermophilus, 45-60 parts of pseudomonas putida, 5-15 parts of rhodopseudomonas palustris, 10-20 parts of bacillus pumilus and 10-40 parts of enterococcus faecium respectively; the compound microbial preparation is prepared by the following steps:
1) respectively fermenting lactobacillus acidophilus, streptococcus thermophilus, pseudomonas putida, rhodopseudomonas palustris, bacillus pumilus and enterococcus faecium by adopting high-density liquid, and preparing the fermentation liquid into bacterial liquid with the viable count of 200 CFU;
2) preparing a compound microbial preparation from the bacterial liquid according to 44 parts of lactobacillus acidophilus, 35 parts of streptococcus thermophilus, 47 parts of pseudomonas putida, 9 parts of rhodopseudomonas palustris, 12 parts of bacillus pumilus and 25 parts of enterococcus faecium, adding apple juice according to the volume ratio of 9%, and uniformly mixing to obtain the microbial preparation.
3. The complex microbial preparation for air deodorization as set forth in claim 2, wherein: the contents of the components are respectively 45 parts of lactobacillus acidophilus, 25 parts of streptococcus thermophilus, 51 parts of pseudomonas putida, 10 parts of rhodopseudomonas palustris, 15 parts of bacillus pumilus and 35 parts of enterococcus faecium.
4. Use of the complex microbial preparation for air deodorization according to claim 1 for purifying air.
5. Use of the complex microbial preparation for air deodorization according to claim 4, wherein: the amount of the deodorization microbial preparation sprayed is 250 ml/ton each time, and the deodorization microbial preparation is sprayed 1 time per week.
CN202010641982.8A 2020-07-06 2020-07-06 Compound microbial preparation for air deodorization and application thereof Pending CN111909865A (en)

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CN113528387A (en) * 2021-07-15 2021-10-22 武汉惠斯顿环保科技有限公司 Microbial preparation, preparation method thereof and application thereof in deodorization
CN114891662A (en) * 2022-03-05 2022-08-12 福建亮洁环境服务有限公司 Pseudomonas putida and application thereof

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113528387A (en) * 2021-07-15 2021-10-22 武汉惠斯顿环保科技有限公司 Microbial preparation, preparation method thereof and application thereof in deodorization
CN113528387B (en) * 2021-07-15 2022-03-08 武汉惠斯顿环保科技有限公司 Microbial preparation, preparation method thereof and application thereof in deodorization
CN114891662A (en) * 2022-03-05 2022-08-12 福建亮洁环境服务有限公司 Pseudomonas putida and application thereof
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