CN101269227A - Biological agent for purifying air and preparation method thereof - Google Patents
Biological agent for purifying air and preparation method thereof Download PDFInfo
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- CN101269227A CN101269227A CNA2007100645853A CN200710064585A CN101269227A CN 101269227 A CN101269227 A CN 101269227A CN A2007100645853 A CNA2007100645853 A CN A2007100645853A CN 200710064585 A CN200710064585 A CN 200710064585A CN 101269227 A CN101269227 A CN 101269227A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses an air purifying biological preparation and a preparation method thereof. The biological preparation of the invention is mainly composed of the following microorganisms by weight portions: 40-60 portions of rhodopseudomonas palustris, 10-30 portions of lactobacillus acidophilus, 10-30 portions of saccharomyces cerevisiae, and 10-30 portions of Jingyang streptomyces. The biological preparation of the invention contains multifold effective microbial floras, the beneficial microorganisms in the microbial inoculums can inhibit the effluvium components from being generated, on the other hand, the microorganisms act on the above components directly, thereby the content of harmful gases is reduced, the consistence of the effluvium is reduced, so that the biological preparation of the invention can quickly reduce the consistence of multifold harmful gases, such as ammonia, hydrogen sulfide, etc., can purify the air with notable deodorization effect, is applicable for deodorizing in culture farms, livestock houses, toilets, septic tanks, sewages, factories, odorous public places, sewage farms, etc.; in addition, the biological preparation of the invention can improve the immunity of the livestock, and can kill pathogen.
Description
Technical field
The present invention relates to a kind of biological preparation, relate in particular to harmful gass such as can degrade ammonia and hydrogen sulfide and have the bacteria agent of certain bactericidal action, the purposes of the invention still further relates to that the preparation method of this bacteria agent and this bacteria agent are applied to remove that the poultry house is inside and outside, foul smell being located in lavatory, anaerobic tank, sewer, soot, processing factory, sewage-farm and public place etc. belongs to microorganism field.
Background technology
Poultry houses is inside and outside, lavatory, anaerobic tank, sewer, soot are the places that is easy to generate foul smell most.Because chicken grouies busy that Organic substances such as suction, defecation, rotten material decompose in the hen house, makes harmful gas increase in the air, not only influences the growth of chicken, and pollutes environment such as ambient atmosphere, water, always a great problem that must solve for the chicken farm.Produce in the gas of stinks, contaminated environment at these, to chicken group and environmental hazard bigger ammonia, hydrogen sulfide etc. are arranged.The animal house foul smell mainly decomposes generation by the poultry fecaluria.The composition of foul smell is very complicated, mainly is made up of methane, organic acid, various alcohols, ammonia, hydrogen sulfide, indole and scatol etc.Ammonia and hydrogen sulfide are the main components of poultry houses harmful gas, and they are decomposed and produced by contain ammonia and sulfurous organic compound in the fecaluria respectively.Under the intensive farm management condition, they are to the harm of poultry body and creating a great impression of colony's fertility performance.Development along with scale animal husbandry; the research of harmful gas and ozone Control is also constantly being carried out in the animal house; main by adding the digestibility of raising poultry such as medicine or probiotic bacteria to feedstuff; reduce the discharge of nitrogen, phosphorus in the defecation; improve daily production management, improve ventilation condition in the house; the generation of control harmful gas and foul smell, in addition, can also by directly in house ground spray or dispenser medicine, biological preparation etc. with purification animal house air.
The present air fresheners that use in public places etc. are removed the preparation of abnormal flavour, mainly are chemicals, have problems such as secondary pollution and persistent period weak point.The biological deodorant that uses beneficial microbe to make can solve the problems referred to above.
The feed cost high usage is low, antibiotic etc is used too much to cause and had high input, and product quality descends, and feces of livestock and poultry causes the wasting of resources and environmental pollution because of its stench can not get effectively utilizing very much, is the global problem that exists on the present aquaculture.
Summary of the invention
Technical problem to be solved by this invention is to overcome the deficiencies in the prior art, harmful gass such as a kind of can effectively degrade ammonia and hydrogen sulfide is provided and has the biological preparation of certain bactericidal action.
Technical problem to be solved by this invention is achieved through the following technical solutions:
A kind of biological preparation that purifies air, mainly form by the component of following weight portion:
Rhodopseudomonas palustris (Rhodopseudomonas palustris) (microbial inoculum) 40-60 part, bacillus acidophilus's (Lactobacillus brevis) (microbial inoculum) 10-30 part, saccharomyces cerevisiae (Saccharomycescerevisiae) (microbial inoculum) 10-30 part and footpath positive streptomycete (5406) (streptomyces jingyanggensis) (microbial inoculum) 10-30 part;
Preferably, the weight portion of each microbial inoculum is: 50 parts of Rhodopseudomonas palustris (Rhodopseudomonaspalustris), 20 parts of bacillus acidophilus (Lactobacillus brevis), 20 parts of saccharomyces cerevisiae (Saccharomyces cerevisiae) and footpath positive streptomycete (streptomycesjingyanggensis) 20 parts.
The positive streptomycete in footpath (streptomyces jingyanggensis) belongs to actinomycetes, also claims 5406, is heterotrophic bacteria, aerobic, the optimum temperature of its growth is 28-32 ℃, and the suitableeest growth pH is about 7.2-7.6, less demanding to nutrition can get up on simple culture medium in growth.The carbon source that needs has starch, dextrin, glucose, maltose and glycerol etc., can utilize peptone, aminoacid, nitrate, ammonium salt, carbamide etc. in the nitrogenous source.Generally all need K, Mg, Fe, Cu, Ca etc.Incubation time is more longer than antibacterial, generally all needs 3-7 days just can grow up to bacterium colony.The positive streptomycete in footpath can also produce other metabolite, as aminoacid, nucleotide, enzyme and enzyme inhibitor, vitamin etc. except producing the antibiotic.
Rhodopseudomonas palustris (Rhodopseudomonas palustris) is a photosynthetic bacteria, rich in proteins, vitamin, ubiquinone and photosynthetic pigments, as feed additive, produce multiple somatomedin, promoting immunity factor, ubiquinone etc., promote the germling growth promoter, can improve survival rate and the rate of increase of animal significantly; The egg fowl of feeding, can improve laying rate, improve the color and luster of eggshell, egg yolk, postpone the chicken moult phase, can also discharge the particular matter that to kill or to suppress other pernicious bacteria and viral growth breeding, can suppress the growth of other pernicious bacteria and virus, and can activate the immune system of body, improve the premunition of animal; In addition, Rhodopseudomonas palustris is degradable organic pollutant effectively, the effect that tool clears the pollution off.
It is heavy and improve food conversion ratio that bacillus acidophilus (Lactobacillus brevis) can increase animal day, the bacillus acidophilus can provide available essential amino acids (as lysine and methionine etc.) and various vitamin (vitamin B complex and K, H etc.) for the host, strengthen the Nutrition and Metabolism of animal, directly promote the growth of animal, also has the biologic activity that improves mineral element, improve microecological environment, cleaning intestinal noxious substance is regulated effects such as digestion immune system.
Saccharomyces cerevisiae (Saccharomyces cerevisiae) belongs to Saccharomycetaceae, unicellular, oval or sphere, tool cell wall, cytoplasma membrane, nucleus are (atomic little, often be difficult for seeing), liquid spore, grain line body and various reserve substance, as oil droplet, glycogen etc.Make wine in industrial being used for, yeast sucks monosaccharide such as glucose, fructose, mannose in the cell, and under the condition of anaerobic, the effect through endoenzyme is decomposed into carbon dioxide and ethanol to monosaccharide.Pharmaceutically, because of yeast is rich in vitamin B, protein and plurality of enzymes, thalline can be made into yeast tablet.The treatment dyspepsia, and can from yeast, extract the raw material of producing nucleic acid analog derivative, coenzyme A, cytochrome C, glutathion and several amino acids.Saccharomyces cerevisiae bacterium colony on the wort agar culture medium is milky, and is glossy, smooth, neat in edge.Asexual propagation is based on budding.Energy glucose fermentation, maltose, galactose and sucrose, unfermentable lactose and 6-(.alpha.-D-galactosido)-D-glucose..
Positive streptomycete can produce antibiotic in the footpath, can effectively kill various pathogenic bacterias, but regulating the animal intestines and stomach function, promote aspect shortage effects such as growth of animal, with itself and bacillus acidophilus, Rhodopseudomonas palustris is compatible, effectively remedied the deficiency of these two kinds of microorganisms aspect killing pathogenic bacteria, bacillus acidophilus and Rhodopseudomonas palustris have also been strengthened simultaneously in the growth that promotes animal, cleaning intestinal noxious substance, regulate the effect that digests aspects such as immune system, improving aspect the premunition of animal with Rhodopseudomonas palustris, collaborative each other, bring out the best in each other, be aided with saccharomyces cerevisiae again, above-mentioned four kinds of microorganism compatibilities are played the promotion growth of animal together altogether, human body immunity improving power, regulating gastointestinal function, the effect of degradable organic pollutant.
Described Rhodopseudomonas palustris (Rhodopseudomonas palustris), bacillus acidophilus (Lactobacillus brevis), saccharomyces cerevisiae (Saccharomyces cerevisiae) and the positive streptomycete in footpath (streptomyces jingyanggensis) all can be bought from commercial channels and obtain, and its specification is a feed grade.
In addition, also can buy from commercial channels and obtain described strain, for example: Guangdong Microbes Inst culture presevation administrative center (address: the mid-way one No. hundred Guangdong Province Institute of Micro-biology of GuangZhou, China city martyr; Http:// www.gimcc.net); China common micro-organisms preservation administrative center (address: No. 13, North No.1 Row, Zhongguancun, Haidian District, Beijing City, Institute of Microorganism, Academia Sinica; Network address http://www.ctcccas.ac.cn); Chinese agriculture microorganism fungus kind preservation administrative center (address: No.12 ,zhongguancun south street,Haidian District, Beijing the Chinese Academy of Agricultural Sciences soil and fertilizer institute culture presevation administrative center, network address
Http:// www.accc.org.cn) etc.; According to culture medium prescription and cultivation temperature that supplier provided,, prepare corresponding microbial inoculum again according to the fermentation process of routine.
The preparation method that the present invention purifies abnormal flavour air biological preparation is as follows:
(1) takes by weighing each component by following weight: Rhodopseudomonas palustris (Rhodopseudomonas palustris) 40-60 part, bacillus acidophilus (Lactobacillus brevis) 10-30 part, saccharomyces cerevisiae (Saccharomyces cerevisiae) 10-30 part and the positive streptomycete in footpath (5406) be 10-30 part (streptomycesjingyanggensis);
(2) with behind above-mentioned each component mix homogeneously promptly.
Contain multiple effective microorganism species in the biological preparation of the present invention, for example, good gas wherein and suspicion gas photosynthetic microorganism can utilize hydrogen sulfide to carry out photosynthesis, the secretions that actinomycetes (good gas) produce is inhibited to pathogenic microorganism, therefore, its purification mainly is that wherein active microorganism produces.Beneficial microbe in the microbial inoculum suppresses above-mentioned foul smell composition generation on the one hand, be the direct effect of these microorganisms on the other hand to mentioned component, thereby reduced harmful gas content, reduced odor concentration, so biological preparation of the present invention can reduce multiple harmful gas concentrations such as ammonia, hydrogen sulfide rapidly, purify air, deodorizing effect is remarkable.Biological preparation of the present invention is to improving the immunity of poultry, and reducing the rate of extremely eliminating also has significant effect, thereby can reduce veterinary drug or antibiotic use.
Biological preparation of the present invention is different from the traditional deodorizer with the spice mask malodors, but suppresses to produce smelly element by microbial action.In addition, biological preparation of the present invention is little ecological pure natural preparation, does not contain chemical constituent, and is without any side effects, can not cause secondary pollution.
The scope of application of biological preparation of the present invention: the place that poultry houses is inside and outside, lavatory, anaerobic tank, sewer, soot, processing factory, sewage-farm etc. are easy to generate foul smell or abnormal flavour.
The using method of biological preparation of the present invention:
1, plant, the deodorization of poultry house: poultry lairage front and back evenly were sprayed onto 300 times of this product dilutions in ground, ceiling, wall, blowdown ditch etc. and locate in three days.With 500 times of this product dilutions, sprayed once later on every 7 to 15 days.
2, lavatory, anaerobic tank, sewer, processing factory or sewage-farm deodorization.With 500 times of this product dilutions, directly spray or be poured onto lavatory urinal, anaerobic tank, sewer, ground etc. and locate.
3, feces, rubbish deodorization.With 500 times of this product dilutions, evenly be sprayed onto on feces, the rubbish.
4, consumption: determine according to the pollutant odor unit, to control the standard that is produced as of stink.But use spray once every day in early stage, consumption can appropriateness strengthen, can be as the case may be after stink is controlled at interval couple of days spray once.But spray area 500-1000m after every liter of deodorizer dilution
2
Points for attention:
1, dilution biological preparation deodorizer of the present invention is advisable with well water or clean river, as using tap water, should place reuse more than 24 hours.
2, biological preparation of the present invention can not mix use with virus killing (worm) agent etc.As using virus killing (worm) agent, should be alternate more than 48 hours.
3, behind the sprinkling biological preparation of the present invention, must guard against water and wash in a large number, in order to avoid influence deodorizing effect.
The specific embodiment
Further describe the present invention by the following examples, it should be understood that these embodiment only are used for the purpose of illustration, never limit the scope of the invention.
The preparation of embodiment 1 biological preparation of the present invention
By following weight take by weighing each component (unit: kg): Rhodopseudomonas palustris 40,
The bacillus acidophilus 10, saccharomyces cerevisiae 10, the positive streptomycete 10 in footpath; With above-mentioned each component mix homogeneously promptly.
Wherein, Rhodopseudomonas palustris, the bacillus acidophilus, the positive streptomycete of saccharomyces cerevisiae and footpath is available from Dalian Sanyi Animal Medicine Co., Ltd., its trade name is " Rhodopseudomonas palustris ", " bacillus acidophilus ", " saccharomyces cerevisiae ", " the positive streptomycete in footpath ", and its specification is a feed grade.
The preparation of embodiment 2 biological preparation of the present invention
By following weight take by weighing each component (unit: kg): Rhodopseudomonas palustris microbial inoculum 60,
Bacillus acidophilus's microbial inoculum 30, saccharomyces cerevisiae bacteria agent 30, the positive drappus microbial inoculum 30 in footpath; With above-mentioned each component mix homogeneously promptly.
Wherein, Rhodopseudomonas palustris, the bacillus acidophilus, the positive streptomycete of saccharomyces cerevisiae and footpath is the development in science and technology company limited available from the Shaanxi heart, its trade name is " Rhodopseudomonas palustris ", " bacillus acidophilus ", " saccharomyces cerevisiae ", " the positive streptomycete in footpath ", and its specification is a feed grade.
The preparation of embodiment 3 biological preparation of the present invention
(unit: kg): the bacillus acidophilus 20, saccharomyces cerevisiae 20, the positive streptomycete 20 in footpath to take by weighing each component by following weight; With above-mentioned each component mix homogeneously promptly
Wherein, Rhodopseudomonas palustris, bacillus acidophilus, saccharomyces cerevisiae, the positive streptomycete in footpath is available from the strong Bioceuticals Inc. of herding in Shandong Province, and its trade name is " Rhodopseudomonas palustris ", " bacillus acidophilus ", " saccharomyces cerevisiae ", " the positive streptomycete in footpath ", and its specification is a feed grade.
The preparation of embodiment 4 biological preparation of the present invention
By following weight take by weighing each component (unit: kg): Rhodopseudomonas palustris microbial inoculum 50,
Bacillus acidophilus's microbial inoculum 20, saccharomyces cerevisiae bacteria agent 20, the positive drappus microbial inoculum 20 in footpath; With above-mentioned each component mix homogeneously promptly.
Wherein, the Rhodopseudomonas palustris strain is available from Guangdong Microbes Inst culture presevation administrative center (http://www.gimcc.net), and its goods number is GIM1.167; Bacillus acidophilus's strain is available from Chinese common micro-organisms preservation administrative center (http://www.ctcccas.ac.cn), and its goods number is IFFI06005; The positive streptomyces species in footpath is available from Chinese agriculture microorganism fungus kind preservation administrative center (http://www.accc.org.cn), and its goods number is Accc 40057; Cereuisiae fermentum, available from Guangdong Microbes Inst culture presevation administrative center (http://www.gimcc.net), its goods number is: GIM2.39.
The preparation of Rhodopseudomonas palustris microbial inoculum: the formulated culture medium that provides by supplier, the Rhodopseudomonas palustris bacterial classification inoculation is cultivated in culture medium, medium slant seed bacterial strain added behind the mineral oil store in 4 ℃ of refrigerators, take out twice of activation before using, bacterial strain after the activation is changed in 250 milliliters of Fructus Solani melongenae bottles and cultivates, and cultivation temperature is 37 ℃, cultivates 48 hours, with sterile saline it is washed when strain growth is good, eluate is the inoculum of seed tank.Add culture medium in 1 ton fermentation tank, it consists of: Semen Maydis powder 20kg, soybean cake powder 20kg, K2HPO47.2kg, KH2PO45.3kg, MgSO40.5kg, Oleum Arachidis hypogaeae semen 2kg, water 45kg, and with said components mix homogeneously, adjust pH to 6.8.To producing a jar inoculation, inoculum concentration is 50kg, begins fermentation from seed tank.Fermentation condition: cultivation temperature is 37 ℃, tank pressure is 0.05mpa/cm2, regularly stir, mixing speed is 220rpm, inoculate after 20 hours and took a sample once, observe the thalli growth situation, whether pollution arranged, after liquid fermentation is finished every 4 hours, in zymocyte liquid, add adsorbent and filler xanthan gum, get the Rhodopseudomonas palustris microbial inoculum.
The preparation of bacillus acidophilus's microbial inoculum: the formulated culture medium that provides by supplier, bacillus acidophilus's bacterial classification inoculation is cultivated in culture medium, medium slant seed bacterial strain added behind the mineral oil store in 4 ℃ of refrigerators, take out twice of activation before using, bacterial strain after the activation is changed in 250 milliliters of Fructus Solani melongenae bottles and cultivates, and cultivation temperature is 37 ℃, cultivates 48 hours, with sterile saline it is washed when strain growth is good, eluate is the inoculum of seed tank.Add culture medium in 1 ton fermentation tank, it consists of: Semen Maydis powder 20kg, soybean cake powder 20kg, K
2HPO
47.2kg, KH
2PO
45.3kg, MgSO
40.5kg, Oleum Arachidis hypogaeae semen 2kg, water 45kg, with said components mix homogeneously, adjust pH to 6.8.To producing a jar inoculation, inoculum concentration is 50kg, begins fermentation from seed tank.Fermentation condition: cultivation temperature is 28 ℃, tank pressure is 0.05mpa/cm2, regularly stir, mixing speed is 220rpm, inoculate after 20 hours and took a sample once, observe the thalli growth situation, whether pollution arranged, after liquid fermentation is finished every 4 hours, in zymocyte liquid, add adsorbent and filler xanthan gum, get bacillus acidophilus's microbial inoculum.
The preparation of the positive drappus microbial inoculum in footpath: the formulated culture medium that provides by supplier, directly positive streptomyces species is inoculated in and cultivates in the culture medium, medium slant seed bacterial strain added behind the mineral oil store in 4 ℃ of refrigerators, take out twice of activation before using, bacterial strain after the activation is changed in 250 milliliters of Fructus Solani melongenae bottles and cultivates, and cultivation temperature is 32 ℃, cultivates 48 hours, with sterile saline it is washed when strain growth is good, eluate is the inoculum of seed tank.Add culture medium in 1 ton fermentation tank, it consists of: Semen Maydis powder 20kg, soybean cake powder 20kg, K
2HPO
47.2kg, KH
2PO
45.3kg, MgSO
40.5kg, Oleum Arachidis hypogaeae semen 2kg, water 45kg, with said components mix homogeneously, adjust pH to 6.8.To producing a jar inoculation, inoculum concentration is 50kg, begins fermentation from seed tank.Fermentation condition: cultivation temperature is 28 ℃, tank pressure is 0.05mpa/cm2, regularly stir, mixing speed is 220rpm, inoculate after 20 hours and took a sample once, observe the thalli growth situation, whether pollution arranged, after liquid fermentation is finished every 4 hours, in zymocyte liquid, add adsorbent and filler xanthan gum, get the positive drappus microbial inoculum in footpath.
The preparation of beer yeast bacteria agent: the formulated culture medium that provides by supplier, the cereuisiae fermentum bacterial classification inoculation is cultivated in culture medium, medium slant seed bacterial strain added behind the mineral oil store in 4 ℃ of refrigerators, take out twice of activation before using, bacterial strain after the activation is changed in 250 milliliters of Fructus Solani melongenae bottles and cultivates, and cultivation temperature is 32-37 ℃, cultivates 48 hours, with sterile saline it is washed when strain growth is good, eluate is the inoculum of seed tank.Add culture medium in 1 ton fermentation tank, it consists of: Semen Maydis powder 20kg, soybean cake powder 20kg, K2HPO47.2kg, KH2PO45.3kg, MgSO40.5kg, Oleum Arachidis hypogaeae semen 2kg, water 45kg, and with said components mix homogeneously, adjust pH to 6.8.To producing a jar inoculation, inoculum concentration is 50kg, begins fermentation from seed tank.Fermentation condition: cultivation temperature is 28 ℃, tank pressure is 0.05mpa/cm2, regularly stir, mixing speed is 220rpm, inoculate after 20 hours and took a sample once, observe the thalli growth situation, whether pollution arranged, after liquid fermentation is finished every 4 hours, in zymocyte liquid, add adsorbent and filler xanthan gum, get the beer yeast bacteria agent.The 1 biological preparation hen house deodorization effect test of the present invention of test example
One, experimental condition and design
1, experimental condition: individual raiser family carries out in Pinggu, Beijing, 15 square metres of test hen house areas, and the commodity egg kind is the enlightening card, goes into 418 of chickling sums, 80 ages in days begin to change the group and go up cage.
2, EXPERIMENTAL DESIGN:, selected a hen house area big but kind, density and the essentially identical hen house of raising condition are contrast nearby, so that relatively in order to measure the deodorization of biological preparation of the present invention.
3, given the test agent: the biological preparation that the embodiment of the invention 1,2,3 and 4 is prepared.
4, test method:
The test hen house: chicken lairage front and back evenly were sprayed onto 300 times of given the test agent dilutions in ground, ceiling, wall, blowdown ditch etc. and locate in three days.With 500 times of given the test agent dilutions, sprayed once later on every 7 to 15 days.
Contrast hen house: do not adopt any measure to carry out deodorization and handle.
Two, result and analysis
1, biological preparation of the present invention can improve the resistance against diseases of laying hen effectively, and mortality rate obviously reduces
After using biological preparation of the present invention, any epidemic situation does not take place in the chicken of test hen house group in whole feeding process, and the chicken food desire is vigorous, and hair color light, hat are blushed profit, the body constitution stalwartness.From 80 ages in days, accumulative total is raised natural law and is reached 540 days, dead eliminates 63 therebetween, month dead mortality 0.8%, and wherein Marek 0.32%, and traumatic death accounts for 0.08%, early eliminates 0.2%, and other only dies of illness 0.2%.Relevant research data shows the minimum 0.6%-0.8% of being of the moon mortality rate of enlightening card laying hen, under local raise competence condition, the moon of statistical average for many years, dead mortality was generally about 1.5%-2%, this shows that the laying hen of the using biological preparation processing of the present invention rate of extremely eliminating reduces more than 1/2 than local, reached the minimum rate level of extremely eliminating, effect is fairly obvious.
2, biological preparation of the present invention can be removed the feces stench, improves environment of chicken house
Because chicken grouies busy that Organic substances such as suction, defecation, rotten material decompose in the hen house, makes harmful gas increase in the air, not only influences the growth of chicken, and pollutes environment such as ambient atmosphere, water, always a great problem that must solve for the chicken farm.Produce in the gas of stinks, contaminated environment at these, to chicken group and environmental hazard bigger ammonia, hydrogen sulfide etc. are arranged.In test we as index, measurement result sees Table 1 with wherein ammonia.
Table 1 biological preparation of the present invention is raised the analysis of laying hen deodorizing effect
Can find out that from table 1 the NH3 concentration in the test group hen house in the NH3 concentration ratio contrast hen house has reduced by 50.3%, ammonia safe level (25ppm) in the hen house.The people works in hen house, feels not smelly naturally, and gas is not dazzling in the house.This shows that biological preparation of the present invention is to improving the environment of chicken house condition, particularly the harmful gas ammonia has very obvious effects in the interior air of house to reducing.
3, chicken group Excreta nitrogen content is higher
By measuring fresh chicken manure nitrogen content, test group hen house chicken manure nitrogen content 1.76%, matched group hen house chicken manure nitrogen content is 1.66%, the test comparison is according to high 0.1 percentage point.This is that the volatilization of ammonia causes in the chicken manure because biological preparation of the present invention has reduced.
Above-mentioned result of the test shows that biological preparation of the present invention can improve the resistance against diseases of laying hen, reduce and extremely to eliminate rate, thereby the use that reduces veterinary drug etc. also has remarkable result.Biological preparation of the present invention has reduced the ammonia concentration in the hen house effectively, has eliminated the stench of feces substantially, to improving the environment of chicken house condition very obvious effects is arranged, also for chicken manure rationally, make full use of the condition created.Application on breeding layer chicken has tangible production, economy and ecological benefits.
Test example 2 biological preparation of the present invention purify the test of animal house air effect
One, materials and methods
1. given the test agent
The biological preparation that the embodiment of the invention 2,3 and 4 is prepared.
2. test method
2.1 given the test agent is to the degradation effect test of pig house ammonia (NH3) and laying hen house hydrogen sulfide (H2S)
Ammonia and concentration of hydrogen sulfide behind the given the test agent of mensuration input successively in the 1h standard pig house.Given the test agent put-on method: by past ground, every m3 space even dispenser 20～25g given the test agent.Ammonia and hydrogen sulfide are measured and are adopted nessler reagent colorimetry and methylene-blue colorimetric method respectively, gas sampling CD-1 type portable type air sampler.5 point samplings in the hurdle house are averaged.
2.2 the Camphora sheet that sell given the test agent and market degraded ammonia contrast test
At 20m
2Two hurdles houses ground on dispenser given the test agent 400g respectively, measure ammonia concentration in each hurdle house air behind the 1h.The ammonia sampling is the same with assay method.
2.3 given the test agent is tested pig hen house deodorizing effect
Select 4 building standard pig houses and 2 building standard hen houses, raise pigs in the pig house, raise chickens gross area 2832m in the hen house
2, air volume is 7132.5m
3, pig house, hen house are in advance by every m
3Given the test agent 56640g is used altogether toward ground dispenser 7～8g given the test agent in the space.Deodorizing effect adopts 6 grades of odor strength point systems according to 5 people's sensation, and asks its meansigma methods.
0 grade: odorless (100 minutes); 1 grade: feel that slightly foul smell is arranged (80 minutes); 2 grades: can feel it is and so on weak smelly (60 minutes); 3 grades: obviously feel foul smell (40 minutes); 4 grades: strong foul smell (having excitement) (20 minutes); 5 grades: strong foul smell, vomiting is arranged, shed tears etc. felt (0 minute).
2.4 given the test agent is to the chicken droppings deodorant effect test
Add chicken manure 250g respectively in 5 beakers, wherein 1 beaker is organized (promptly not adding ecological bacteria agent of century) in contrast, wherein 4 beakers add 2.5,5.5,7.5 respectively, the 10.5g given the test agent.Divide every day and measure the odor concentration that each is organized early, middle and late 3 times, last 3d.Odor concentration assay method:, adopt 6 grades of odor strength point systems then by the foul smell of 5 each beaker of human nasil.
2.5 bacteriostatic test in the given the test agent house
Test is carried out in the hurdle of 20m2 house.Initial bacterial population is given up on this hurdle before elder generation's determination test, measures the bacterial population that the 1h hurdle is given up behind the dispenser 400g given the test agent of ground again.The fall mensuration of bacterium number of hurdle house air adopts the plain agar colony counting method, and 4 samplings are averaged.
Two, result of the test
1. given the test agent is to the degradation effect of air ammonia in the pig house
Table 2 shows, throws in the interior ammonia concentration of the preceding house of given the test agent and on average reaches 58.8mg/m
3, behind the input given the test agent, ammonia concentration on average drops to 16.1mg/m in the house
3, degradation rate reaches 72.5%.Pig house ammonia sanitary standard is 19.5mg/m
3Explanation in pig house, use given the test agent can make the house in ammonia concentration be controlled in the sanitary standard.
Table 2 is thrown in the degradation effect of given the test agent to air ammonia in the pig house
| Test number (TN) | 1 | 2 | 3 | 4 | Average |
| Ammonia concentration/mg.m in the house before throwing in 3 | 66.8 | 57.5 | 48.4 | 62.6 | 58.8±7.9 |
| Give up interior ammonia concentration/mg.m after throwing in given the test agent 3 | 17.5 | 16.1 | 14.2 | 16.5 | 16.1±1.4 |
| Ammonia degradation rate in the house before and after throwing in/(%) | 73.8 | 72.0 | 70.7 | 73.4 | 72.5±1.4 |
2. given the test agent is to the degradation effect of sulfuration ammonia in the laying hen house
Table 3 shows, throws in the interior ammonia concentration of the preceding house of given the test agent and on average reaches 58.8mg/m
3Surpassed animal house hydrogen sulfide 16.1mg/m
3Sanitary standard.After throwing in given the test agent, concentration of hydrogen sulfide on average drops to 3.9mg/m
3, degradation rate reaches 81.5%, and reaches hygienic requirements.
Table 3 is thrown in the degradation effect of given the test agent to hydrogen sulfide in the laying hen house
| Test number (TN) | 1 | 2 | 3 | 4 | Average |
| Ammonia concentration/mg.m in the house before throwing in 3 | 20.4 | 22.8 | 19.8 | 20.2 | 20.8±1.4 |
| Give up interior ammonia concentration/mg.m after throwing in given the test agent 3 | 3.9 | 4.2 | 3.5 | 3.8 | 3.9±0.3 |
| Ammonia degradation rate in the house before and after throwing in/(%) | 80.9 | 81.6 | 82.3 | 81.2 | 81.5±0.6 |
3. given the test agent and commercially available Camphora sheet are to ammonia effect contrast test in the degraded animal house
Table 4 shows, compares with the blank group, and given the test agent and Camphora sheet are all effective to ammonia degraded in the animal house.The given the test agent group can reach 60.8% to the ammonia degradation rate, average out to 57.6%; Degraded is up to 52.2% and Camphora sheet group is to ammonia, and average out to 44.8% illustrates that given the test agent is stronger than Camphora sheet to the ammonia degraded.
Ammonia effect in table 4 given the test agent and the Camphora sheet degraded animal house
4. given the test agent is to the deodorizing effect of pig hen house
Table 5 shows, every m
3Pig house, hen house space throw in the 20g given the test agent after, according to 6 grades of odor strengths scorings, average mark belongs to 0～1 grade of odor strength more than 86 minutes.Illustrate that given the test agent knot pig hen house has deodorizing effect preferably.
Table 5 given the test agent is to pig hen house deodorization appraisal result
5. given the test agent is to the chicken droppings deodorant effect
In observing time, do not throw in the beaker of given the test agent and send carrion foul smell, and reach and stimulate the degree of shedding tears, belong to 5 grades of odor strengths; Add in the beaker of 2.5g given the test agent, also obviously feel stink, belong to 3 grades of odor strengths; Add in the beaker of 5.5g given the test agent, only feel to have foul smell slightly, belong to 1 grade of odor strength; In the beaker of interpolation 7.5 and 10.5g given the test agent, do not realize stink, belong to 0 grade of odor strength.
6. given the test agent reduces bacterial population effect in the animal house
Table 6 shows that 4 bacterium that fall add up to 80 in the 1st the preceding animal house of (culture medium is air exposure 10min in house) input given the test agent, and behind the input given the test agent, 4 bacterium that fall add up to 68, have reduced 12, and its reduction rate is 15%.But 4 bacterium that fall altogether add up to 102 in the animal house before the 2nd (culture medium is air exposure 20min in house) test, after throwing in given the test agent, 4 bacterium that fall add up to 96, have reduced 6, its reduction rate is 5.9%, and given the test agent airborne bacterial population DeGrain in reducing house is described.
Table 6 is thrown in given the test agent and is reduced bacterial population effect in the animal house
Result of the test shows that given the test agent degraded animal house ammonia and hydrogen sulfide are respond well, and the given the test agent dispenser can both be controlled stink preferably at feces and She Nei, is a kind of house of poultry preferably air purifying preparation; In addition, aspect the bacterial population of given the test agent in suppressing animal house certain effect is arranged also.
Test example 3 biological preparation of the present invention reduce ammonia, formaldehyde and benzene concentration test
1, for the prepared biological preparation of test agent: embodiment of the invention 1-4.
2, inspection machine: 722 spectrophotometers; The QT-2 air sampler.
3, inspection item: ammonia, formaldehyde and benzene.
4, testing conditions: 1.5m
3Air chamber.
5, test method:
Test is at 6 airtight 1.5m
3Test chamber in carry out, 3 is the blank cabin, in addition 3 for for the test agent cabin;
At 3 1m
2Paper substrate on, will be for test agent spraying 2 times, dry the back repaste naturally the 2nd time, 3 paper substrates of handling well be suspended on in the test agent cabin for the 1st time;
The ammonia source of release is put in respectively in 2 cabins tends to balance, open fan, make ammonia and cabin in behind the abundant mix homogeneously of air, close electric fan, sample and the initial concentration of mensuration ammonia; 24 hours post-samplings are also measured ammonia concentration in 2 cabins.
The assay method of formaldehyde and benzene and the assay method of above-mentioned ammonia are just the same.
6, result of the test:
Testing result after 24 hours:
Result of the test shows that biological preparation of the present invention can significantly reduce the concentration of harmful gass such as ammonia in the air, formaldehyde and benzene, and the effect that purifies air is obvious.
Claims (8)
1. the biological preparation of place air such as purifying aquaculture field, poultry house, lavatory, anaerobic tank, sewer, processing space, the public place that abnormal flavour is arranged and sewage-farm, mainly form: Rhodopseudomonas palustris (Rhodopseudomonas palustris) 40-60 part by the component of following weight portion, bacillus acidophilus (Lactobacillusbrevis) 10-30 part, saccharomyces cerevisiae (Saccharomyces cerevisiae) 10-30 part and footpath positive streptomycete (streptomyces jingyanggensis) 10-30 part.
2. according to the biological preparation of claim 1, it is characterized in that, the weight portion of each component is: 50 parts of Rhodopseudomonas palustris (Rhodopseudomonas palustris), 20 parts of bacillus acidophilus (Lactobacillus brevis), 20 parts of saccharomyces cerevisiae (Saccharomyces cerevisiae) and footpath positive streptomycete (streptomycesjingyanggensis) 20 parts.
3. method for preparing the biological preparation of claim 1 comprises:
(1) take by weighing each component by following weight:
Rhodopseudomonas palustris 40-60 part
Bacillus acidophilus 10-30 part
Saccharomyces cerevisiae 10-30 part
The positive streptomycete 10-30 part in footpath;
(2) with above-mentioned each component mix homogeneously, promptly.
4. claim 1 or 2 the application of biological preparation in removing plant, poultry house, lavatory, anaerobic tank, sewer, processing space, sewage-farm, feces, rubbish and public place stink, comprise: behind the biological preparation dilute with water with claim 1 or 2, directly spray or be poured onto the institute object to be processed on.
5. according to the application of claim 4, it is characterized in that, comprising: poultry lairage front and back evenly were sprayed onto ground, ceiling, wall, blowdown ditch with behind 300 times of the described biological preparation dilute with waters in three days; With 500 times of described biological preparation dilute with waters, sprayed once later on every 7 to 15 days.
6. according to the application of claim 4, it is characterized in that, comprising:, directly spray or be poured onto lavatory urinal, anaerobic tank, sewer or ground 500 times of described biological preparation dilute with waters.
7. according to the application of claim 4, it is characterized in that, comprising:, evenly be sprayed onto on feces, the rubbish 500 times of described biological preparation dilute with waters.
8. claim 1 or 2 the biological preparation application in killing plant, poultry house, lavatory antibacterial comprises: behind the biological preparation dilute with water with claim 1 or 2, directly spray or be poured onto on institute's object to be processed.
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