CN1300299C - Farm composite bacteria prepn and preparing method - Google Patents
Farm composite bacteria prepn and preparing method Download PDFInfo
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- CN1300299C CN1300299C CNB2004100745449A CN200410074544A CN1300299C CN 1300299 C CN1300299 C CN 1300299C CN B2004100745449 A CNB2004100745449 A CN B2004100745449A CN 200410074544 A CN200410074544 A CN 200410074544A CN 1300299 C CN1300299 C CN 1300299C
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Abstract
The present invention discloses an agricultural composite bacteria agent and a preparation method thereof. The agricultural composite bacteria agent is prepared from 60% of lactobacillus acidophilus, 30% of bacillus subtilis and 10% of saccharomycete cerevisiae through combination, sieving, inoculation according to steps respectively in a nutrient fluid, and aerobic and anaerobic mixed culture at certain temperature. The agricultural composite bacteria agent of the present invention has the advantages of wide range of use, good performance, stable effect, no toxicity, no harm and low cost. The provided preparation method has the advantages of simple manufacturing process, practicability, short preparation period and low cost. The present invention can be widely applied to agricultural planting and animal cultivation so as to enhance the output of agronomic crops and fowls, enhance immunity and prevent pest and disease damage. As an essential product of a nuisance free green food, the agricultural composite bacteria agent has obvious economical benefits and social benefits.
Description
Affiliated technical field:
The present invention relates to microorganism live bacteria fungicide preparation technical field, particularly relate to a kind of preparation method of farm composite bacteria preparation.
Background technology:
Needs along with the ecological agriculture development globalization, the traditional concept that applying method and livestock and poultry cultivation are ploughed in traditional plantation changes just gradually, when increasing crop yield and culturing income, greatly develop the green non-pollution agricultural and become inexorable trend, and the application of biotechnology in modern agricultural is also more general, China is a large agricultural country, in the development of Organic farming and pollution-free food production, then can increase day by day the demand of agricultural microorganism microbial inoculum.But the product of market sale in recent years exists some defectives more or less, causes it the problem that some are difficult to overcome to occur at products production or aspect applying.For example: from the EM microbial inoculum product of Japan's introduction, form because it is known as by 80 kinds of bacterium cultivations, this products production cost and selling price height limit its large scale application, particularly the classification position of tens of its use kinds of bacterium is indeterminate, also can't carry out clear and definite quality product registration.And for example: the microbial inoculum product that China applies at present, be that single culture is cultivated the microbial inoculum that produces mostly, comprise: azotogen, phosphorus bacteria microbial inoculum, silicate bacteria microbial inoculum, yeast microbial inoculum, photosynthetic bacteria microbial inoculum etc., because single bacterial classification microbial inoculum performance is single, result of use is very unstable, problem based on above-mentioned two series products existence, how to pass through the excellent species combined sorting, effect stability is produced in cultivation, cheap farm composite bacteria preparation and preparation method are the important topics in current this area.
Summary of the invention:
The objective of the invention is by from existing all kinds of bacterial classifications, cultivating, thereby a kind of preparation method that can produce excellent property, effect stability, nontoxic and cheap farm composite bacteria preparation is provided through screening combination.
In order to achieve the above object, the present invention adopts following technical scheme, a kind of preparation method of farm composite bacteria preparation, be to make through mixed culture by the Lactobacterium acidophilum (Lactobacillus acidophilus) of 50-70%, the bacillus subtilis (Bacillus subtilis) of 20-40% and three kinds of bacterium of S. cervisiae (Saccharomyces cerevisiae) of 5-15%, in order to prepare above-mentioned described composite fungus agent, the processing method and the step that are adopted are as follows:
(1) to contain sugar be 5% nutritive medium in configuration, and the sterilization back is standby;
(2) in nutritive medium behind the inoculation S. cervisiae, and it is standby to place under 28 ℃ of-30 ℃ of temperature aerobic to cultivate after 48 hours;
(3) in the nutritive medium of the S. cervisiae of handling behind the inoculation bacillus subtilis, and place 28 ℃ of-30 ℃ of temperature aerated culture standby after 48 hours through step (2);
(4) to the S. cervisiae of handling and the nutritive medium relaying continued access kind Lactobacterium acidophilum of bacillus subtilis, and place under 28 ℃ of-30 ℃ of temperature anaerobism to cultivate 7-10 days, behind pH≤4.0, stop to cultivate, thereby form farm composite bacteria preparation through step (3).
Above-mentioned farm composite bacteria preparation, its pH≤4.0, every milliliter of quantity that contains effective bacterium is hundred million of 2.0-5.0.
The composition of above-mentioned nutritive medium is that clear water adds 5% sugar formation.
The purchase of above-mentioned bacterial classification Lactobacterium acidophilum (Lactobacillus acidophilus), bacillus subtilis (Baciiiussubtilis) and S. cervisiae (Saccharomyces cerevisiae) is numbered:
The numbering of Lactobacterium acidophilum-1.2467;
The numbering of bacillus subtilis-1.933;
The numbering of S. cervisiae-2.614.
Effect of the present invention is exactly diseases prevention and promotion growth in agriculture animals and plants, its mechanism of action is to keep the balance of beneficial microorganism system in the plant and animal body and the effect of having given play to its maximum, the present invention is widely used in the breed of agriculture plant growing and animal, plant growing comprises proportion of crop planting, the fruits and vegetables plantation, its using method is this composite fungus agent to be converted 300 times of water directly plant leaf surface is sprayed, or and fertilizer stir and to carry out the plant fertilising, no matter be to spray or its effect of topdressing is identical, all can obtain tangible growth to growth and the output of plant, experiment shows, can improve output greatly after vegetables and fruit and food crop are used this composite fungus agent, its amount of increase in production reaches as high as 50% more than 6-15%.And can quicken soil nutrient and transform, improve available nutrient of soil content, improve soil physical and chemical property, improve the crop disease-resistant ability, except that increasing output, also can improve product quality.Aspect livestock and poultry cultivation and aquaculture, using method also is this composite fungus agent to be converted behind 300 times of water drink directly or stir that into feed is directly edible to animal, all can obtain tangible growth to the growth of animal, experiment shows that to animal edible or after drinking this composite fungus agent, the average growth rate of its animal is more than 10%, the animal marketing period shortens 10%, in addition also can obviously strengthen animal immunizing power and disease resistance, the animal dead rate on average reduces by 15%, and effect is very remarkable.In addition, this composite fungus agent also has environmental purification function, can remove the stink of feces of livestock and poultry, also can be used for the purifying treatment of domestic waste and sewage.This composite fungus agent do not contain any chemical objectionable impurities, has no side effect, free from environmental pollution, be the ideal product of exploitation pollution-free green food, remarkable economic efficiency and social benefit are arranged.
Farm composite bacteria preparation use range provided by the present invention extensively, excellent property, effect stability, nontoxic and cheap, and the preparation method's technology that is provided is simple, practical, preparation cycle is short and cost is low.
Description of drawings:
Fig. 1 is a process flow diagram of the present invention
Embodiment:
Below in conjunction with embodiment in detail the present invention is described in detail: component content of the present invention as can be seen from Figure 1 and preparation method, a kind of farm composite bacteria preparation, the Lactobacterium acidophilum by 60% (Lactobacillus acidophilus), 30% bacillus subtilis (Bacillus subtilis) and three kinds of bacterium of S. cervisiae (Saccharomyces cerevisiae) of 10% are made through mixed culture.In order to prepare above-mentioned described composite fungus agent, the processing method and the step of employing are as follows:
1. configuration contains the nutritive medium of sugar 5%, and the sterilization back is standby;
2. in nutritive medium, inoculate S. cervisiae, and it is standby to place under 29 ℃ of temperature aerobic to cultivate after 48 hours;
3. in the nutritive medium of the S. cervisiae of handling, inoculate bacillus subtilis, and place under 29 ℃ of temperature aerated culture standby after 48 hours through step (2);
4. to the S. cervisiae of handling and the nutritive medium relaying continued access kind Lactobacterium acidophilum of bacillus subtilis through step (3), place under 29 ℃ of temperature anaerobism to cultivate 8 days, behind pH≤4.0, stop to cultivate, thereby form farm composite bacteria preparation.
Claims (3)
1. the preparation method of a farm composite bacteria preparation, be to make through mixed culture by the Lactobacterium acidophilum (Lactobacillusacidophilus) of 50-70%, the bacillus subtilis (Bacillus subtilis) of 20-40% and three kinds of bacterium of S. cervisiae (Saccharomyces cerevisiae) of 5-15%, it is characterized in that: the preparation method of described farm composite bacteria preparation, its step is as follows:
(1) to contain sugar be 5% nutritive medium in configuration, and the sterilization back is standby;
(2) in nutritive medium behind the inoculation S. cervisiae, and it is standby to place under 28 ℃ of-30 ℃ of temperature aerobic to cultivate after 48 hours;
(3) in the nutritive medium of the S. cervisiae of handling behind the inoculation bacillus subtilis, and place 28 ℃ of-30 ℃ of temperature aerated culture standby after 48 hours through step (2);
(4) to the S. cervisiae of handling and the nutritive medium relaying continued access kind Lactobacterium acidophilum of bacillus subtilis, and place under 28 ℃ of-30 ℃ of temperature anaerobism to cultivate 7-10 days, behind pH≤4.0, stop to cultivate, thereby form farm composite bacteria preparation through step (3).
2. the preparation method of farm composite bacteria preparation according to claim 1, it is characterized in that: above-mentioned farm composite bacteria preparation, every milliliter of quantity that contains effective bacterium is hundred million of 2.0-5.0.
3. the preparation method of farm composite bacteria preparation according to claim 1 is characterized in that: the composition of the nutritive medium in the above-mentioned steps (1) is that clear water adds 5% sugar and constitutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100745449A CN1300299C (en) | 2004-09-08 | 2004-09-08 | Farm composite bacteria prepn and preparing method |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2004100745449A CN1300299C (en) | 2004-09-08 | 2004-09-08 | Farm composite bacteria prepn and preparing method |
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| Publication Number | Publication Date |
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| CN1587372A CN1587372A (en) | 2005-03-02 |
| CN1300299C true CN1300299C (en) | 2007-02-14 |
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| CNB2004100745449A Expired - Fee Related CN1300299C (en) | 2004-09-08 | 2004-09-08 | Farm composite bacteria prepn and preparing method |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100465263C (en) * | 2006-05-12 | 2009-03-04 | 北京沃土天地生物科技有限公司 | Composite microorganism foliage fertilizer bacteria agent and its producing method and use |
| CN100465264C (en) * | 2006-05-12 | 2009-03-04 | 北京沃土天地生物科技有限公司 | Composite microorganism bacteria agent for compost fermentation and its producing method and use |
| CN101268804B (en) * | 2007-03-21 | 2011-02-02 | 北京世纪星伊埃姆生物技术研究所 | Microbial feed additive, preparation method and application thereof |
| CN101269227B (en) * | 2007-03-21 | 2011-07-27 | 北京世纪星伊埃姆生物技术研究所 | Biological agent for purifying air, preparation method and uses thereof |
| CN101671205B (en) * | 2009-09-27 | 2013-05-29 | 上海创博生物技术有限公司 | Composite microbial preparation for promoting growth of rape and preparation method thereof |
| CN101905985B (en) * | 2010-07-30 | 2012-11-28 | 河北省微生物研究所 | Method for preparing microorganism-decomposing agent used for producing biological organic fertilizer by livestock and poultry manure fermentation |
| CN102318779A (en) * | 2011-10-18 | 2012-01-18 | 上海创博生态工程有限公司 | Small peptide additive for porcine feed and preparation method thereof |
| CN107439600A (en) * | 2017-07-04 | 2017-12-08 | 北京百丰天下生物科技有限公司 | New alternative flower thinning preparation and preparation method thereof |
| CN110452844A (en) * | 2019-08-15 | 2019-11-15 | 陈玉亭 | A kind of preparation method of the complex micro organism fungicide for waste harmless treatment |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20020066079A (en) * | 2001-02-09 | 2002-08-14 | 강석훈 | Novel microorganism which effectively enhances animal performances and reduces smell of animal excretions, agent containing thereof and preparation method of the same |
| KR20030065683A (en) * | 2002-01-30 | 2003-08-09 | 주식회사 한빛바이오 | Fermented Feeds Containing Ume and Their Preparation Method |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20020066079A (en) * | 2001-02-09 | 2002-08-14 | 강석훈 | Novel microorganism which effectively enhances animal performances and reduces smell of animal excretions, agent containing thereof and preparation method of the same |
| KR20030065683A (en) * | 2002-01-30 | 2003-08-09 | 주식회사 한빛바이오 | Fermented Feeds Containing Ume and Their Preparation Method |
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