KR101669599B1 - Composition for eliminating odor and heavy metal comprising effective microorganism culture broth as effective component - Google Patents

Abstract

Provided are a method for manufacturing a fermented liquid fertilizer containing less heavy metal by using effective microorganisms, which comprises the following steps: (a) preparing a culture fluid of effective microorganisms by culturing effective microorganisms; (b) collecting a fermented fluid by adding the culture fluid of effective microorganisms prepared at the step (a) and a carbon source to muck and fermenting the same, then conducting solid-liquid separation thereof; and (c) adding the culture fluid of effective microorganisms prepared at the step (a) and a carbon source to muck and fermenting the same, and adding the culture fluid of effective microorganisms prepared at the step (a), the fermented fluid, which has gone through the solid-liquid separation at the step (b), and the carbon source to the fermented fluid, which has gone through a first fermentation process conducted by adding the culture fluid of effective microorganisms prepared at the step (a) and fermenting the same, so as to secondarily ferment the same, and a composition for eliminating an odor and heavy metals containing the liquid fertilizer with less heavy metals using effective microorganisms and the culture fluid of effective microorganisms manufactured by the same method.

Description

TECHNICAL FIELD The present invention relates to a composition for removing odors and heavy metals containing an effective microbial culture liquid as an effective ingredient,

(A) culturing a useful microorganism to prepare a useful microorganism culture solution; (b) adding to the animal manure the useful microorganism culture solution and carbon source prepared in the step (a), and fermenting the fermented liquid to obtain a fermented product; And (c) fermenting the livestock manure by adding the culture medium of the useful microorganism and the carbon source prepared in the step (a), adding the culture medium of the useful microorganism prepared in step (a) Wherein the fermentation product is prepared by adding the carbon source and the fermented product obtained in step (b) to the fermented product of the useful microorganism prepared in step (a), followed by secondary fermentation. And a composition for removing heavy metals containing as an active ingredient a fermented broth having a reduced heavy metal content and a useful microorganism culture solution using the useful microorganism produced by the above method.

Fertilizers, rather than direct nutrients of crops, improve the physical chemistry of soils and promote beneficial microorganisms, changing the form of nutrients that could not be used in plants to available forms in the soil, reducing the toxicity of toxic substances indirectly It is a substance that helps crops grow.

These fertilizers are classified into fertilizers and special fertilizers in accordance with Korea's Fertilizer Control Law. Specifically, ordinary fertilizers are classified into inorganic fertilizers (inorganic nitrogen fertilizers, inorganic fertilizers, etc.) based on the three elements of nitrogen, phosphoric acid, It is classified into mineral fertilizer, inorganic potassium fertilizer), compound fertilizer, organic fertilizer, calciferous fertilizer, silicate fertilizer, magnesium fertilizer and boron fertilizer. Special fertilizer includes classified fertilizer However, it does not comply with certain standards and is classified as necessary.

In general, chemical fertilizers are promoting organic agriculture by acidifying soils and polluting groundwater. This organic cultivation can be used to provide safe crops from pesticides by using livestock manure, and also to effectively utilize a large amount of manure generated from livestock housing, thereby reducing the cost of facilities and costs associated with manure disposal .

Livestock manure generated from livestock housing can promote the growth of cultivated crops and increase the taste and sugar content of the fruit. In itself, even if it is used as an organic fertilizer, a great effect can not be expected. And then used as fertilizer. However, there is a problem that odor is generated in the process of composting livestock manure, and heavy metals contained in livestock manure can accumulate in plants when used as fertilizer.

Korean Patent Registration No. 1441005 discloses a method for producing a plant growth promoting solution, and Korean Patent No. 1156650 discloses a method for producing an aqueous solution of a fertilizer by removing odors from an animal manure by a water-soluble corrosion acid. However, This method is different from the method of producing livestock manure fermentation broth with reduced odor and heavy metal content using useful microorganisms.

Disclosure of the Invention The present invention has been made in view of the above-described needs, and it is an object of the present invention to provide a method for producing a liquid fertilizer using livestock manure, which effectively removes odors generated from livestock manure and reduces the heavy metal content contained in the manure, A method for producing a fermented broth using a useful microorganism capable of effectively increasing plant growth, and a composition for removing heavy metals containing an effective microbial culture as an effective ingredient.

In order to solve the above problems, the present invention provides a method for culturing a useful microorganism, comprising: (a) culturing a useful microorganism to prepare a useful microorganism culture; (b) adding to the animal manure the useful microorganism culture solution and carbon source prepared in the step (a), and fermenting the fermented liquid to obtain a fermented product; And (c) fermenting the livestock manure by adding the culture medium of the useful microorganism and the carbon source prepared in the step (a), adding the culture medium of the useful microorganism prepared in step (a) Wherein the fermentation product is prepared by adding the carbon source and the fermented product obtained in step (b) to the fermented product of the useful microorganism prepared in step (a), followed by secondary fermentation. And a fermentation broth of the fermented broth.

The present invention also provides a fermented broth having a reduced heavy metal content by using the useful microorganisms produced by the above method.

In addition, the present invention Lactobacillus bacteria parapa than varnish (Lactobacillus parafarraginis), Lactobacillus para casei (Lactobacillus paracasei , Lactobacillus tolerans , Lactobacillus < RTI ID = 0.0 > buchneri , Lactobacillus < RTI ID = 0.0 > harbinensis , Lactobacillus perolens , Lactobacillus < RTI ID = 0.0 > rhamnosus , Lactobacillus < RTI ID = 0.0 > vaccinostercus ), Acetobacter lovaniensis ), Acetobacter peroxydans , Pichia fermentans ), Candida ethanolica ( Candida ethanolic and Saccharomycopsis schoenii . The present invention also provides a composition for removing heavy metals containing an effective microbial culture as an active ingredient.

The useful microorganism culture liquid of the present invention is excellent in the effect of removing heavy metals and can be usefully used for the purpose of reducing heavy metals contained in the soil and water quality.

In addition, if conventional livestock manure is fermented and used as a fertilizer, it can be sprayed on crops after fermentation for 2 years. In the present invention, the fermentation period can be shortened to within 45 days by fermenting using specific kinds of useful microorganisms have.

In addition, in the composting process, the odor of livestock manure can be effectively removed, and it can be sprayed with water and diluted irrespective of the season of the four seasons, effectively reducing the heavy metals contained in livestock manure and replacing pesticides and chemical fertilizers, Can be provided.

Fig. 1 shows an inspection report of fermented broth of the present invention.

In order to achieve the object of the present invention,

(a) Lactobacillus < RTI ID = 0.0 > parafarraginis , Lactobacillus < RTI ID = 0.0 > paracasei , Lactobacillus tolerans , Lactobacillus < RTI ID = 0.0 > buchneri , Lactobacillus < RTI ID = 0.0 > harbinensis , Lactobacillus perolens , Lactobacillus < RTI ID = 0.0 > rhamnosus , Lactobacillus < RTI ID = 0.0 > vaccinostercus ), Acetobacter lovaniensis ), Acetobacter peroxydans , Pichia fermentans ), Candida ethanolica ( Candida ethanolica ) and Saccharomycopsis schoenii to prepare a useful microorganism culture solution;

(b) adding to the animal manure the useful microorganism culture solution and carbon source prepared in the step (a), and fermenting the fermented liquid to obtain a fermented product; And

(c) fermenting the livestock manure by adding the culture medium of the useful microorganism prepared in the step (a) and the carbon source, and adding the culture medium of the useful microorganism prepared in step (a) to the fermented material after the primary fermentation Wherein the fermented product of step (b) is fermented by adding a carbon source and a carbon source to the fermented product of step (b). The fermented product of step (b) A method for producing a fermented liquid ratio is provided.

In the method of manufacturing fermented broth of the present invention, the carbon source may be preferably a potato starch, and the potato starch is advantageous in that the cost is lower than other carbon sources and the culture effect of the useful microorganism is excellent.

In addition, in the method for producing the fermented broth of the present invention, the livestock manure may preferably be manure of at least one animal selected from the group consisting of pigs, cows, chickens, ducks, horses and dogs, but is not limited thereto.

Further, in the fermentation broth ratio production method of the present invention, the fermentation broth ratio of the present invention is characterized in that the heavy metal content is reduced, and the heavy metal is one or more heavy metals selected from the group consisting of lead, chromium, copper, But is not limited thereto.

The method of the present invention for producing fermented broth comprises, more specifically,

(a) Lactobacillus < RTI ID = 0.0 > parafarraginis , Lactobacillus < RTI ID = 0.0 > paracasei , Lactobacillus tolerans , Lactobacillus < RTI ID = 0.0 > buchneri , Lactobacillus < RTI ID = 0.0 > harbinensis , Lactobacillus perolens , Lactobacillus < RTI ID = 0.0 > rhamnosus , Lactobacillus < RTI ID = 0.0 > vaccinostercus ), Acetobacter lovaniensis ), Acetobacter peroxydans , Pichia fermentans ), Candida ethanolica ( Candida ethanolica and Saccharomycopsis schoenii were mixed and cultured at 23 to 35 ° C. for 92 to 140 hours to prepare a culture medium of useful microorganisms at a concentration of 1 × 10 ^{6 to 8} CFU / ml step;

(b) adding a fermented product to the fermented manure for 25 to 35 days at 25 to 37 ° C. after adding the carbon source and the fermented microorganism culture liquid prepared in step (a), and solid-liquid separation to obtain a fermented product; And

(c) 16-24 L of the useful microorganism culture prepared in step (a) and 12-18 kg of carbon source are added to 15-25 tons of livestock manure, and fermented at 25-37 ° C for 1-2 days, 16-24 L of the useful microorganism culture prepared in the step (a), 16-24 L of the bacterium (b), and 1 to 2 L of the cultured microorganism were added to the fermented product subjected to the first fermentation for 4 to 6 days, ) Fermented at a temperature of 25-37 ° C for 7 to 10 days while adding 1.6 ~ 2.4 tons of fermented solid and 12 ~ 18 kg of carbon source once or twice a day. Repeating 2 to 3 times,

More specifically,

(a) Lactobacillus < RTI ID = 0.0 > parafarraginis , Lactobacillus < RTI ID = 0.0 > paracasei , Lactobacillus tolerans , Lactobacillus < RTI ID = 0.0 > buchneri , Lactobacillus < RTI ID = 0.0 > harbinensis , Lactobacillus perolens , Lactobacillus < RTI ID = 0.0 > rhamnosus , Lactobacillus < RTI ID = 0.0 > vaccinostercus ), Acetobacter lovaniensis ), Acetobacter peroxydans , Pichia fermentans ), Candida ethanolica ( Candida ethanolica and Saccharomycopsis schoenii and then cultured at 25 ° C. for 120 hours to prepare a culture solution of useful microorganisms at a concentration of 1 × 10 ⁷ CFU / ml;

(b) adding a fermented product to the fermented manure for 25 to 35 days at 25 to 37 ° C. after adding the carbon source and the fermented microorganism culture liquid prepared in step (a), and solid-liquid separation to obtain a fermented product; And

(c) To 20 tons of livestock manure, 20 L of the useful microorganism culture prepared in the above step (a) and 15 kg of carbon source were added, followed by fermentation at 25 to 37 DEG C for 1 day. 20 L of the useful microorganism culture was added 20 L of the useful microorganism culture prepared in the step (a), 2 tons of the fermented solid separated in the step (b) and 15 kg of the carbon source were added to the fermented product subjected to the first fermentation process, And repeating the first and second fermentation processes for 2 to 3 times at 25 to 37 DEG C for 7 to 10 days for secondary fermentation.

In the method for producing the fermentation broth of the present invention, the useful microorganism of step (a) is selected from the group consisting of Thiobacillus thiooxidans , Bacillus thuringiensis , Weissella < RTI ID = 0.0 > cibaria , bicellapramesenteroid ( Weissella paramesenteroides and Bacillus amyloliquefaciens . When the useful microorganism culture is added to the livestock manure and fermented, the odor of the livestock manure and Not only the heavy metals can be removed more effectively but also the liquid product can be manufactured by fermenting the livestock manure for a short period of time.

Since the fermented broth of the present invention contains a useful microorganism, it exhibits the function of the pesticide as well as the function of the fertilizer. In other words, if the liquid fertilizer is used for crop cultivation due to the useful microbial fermentation, sufficient nutrients can be supplied and the effect of improving the land can be obtained. Especially, antagonistic microorganisms can protect the crop from diseases or germs So that it is more effective for the soil in which the crop damage is caused. Therefore, when the liquid fertilizer of the present invention is used, crops can be protected from diseases or insect pests without using chemically produced pesticides, so that the yields can be increased, and high quality crops can be cultivated and supplied to consumers. In particular, since it is possible to protect the plant from diseases or insects without using chemical pesticides, environmental pollution can be prevented and ecosystem can be prevented from being destroyed.

The present invention also provides a fermented broth having a reduced heavy metal content using the useful microorganisms produced by the above method. The fermentation broth can be used according to a method commonly used in the art.

The present invention also provides Lactobacillus parapa than varnish (Lactobacillus parafarraginis), Lactobacillus para casei (Lactobacillus paracasei , Lactobacillus tolerans , Lactobacillus < RTI ID = 0.0 > buchneri , Lactobacillus < RTI ID = 0.0 > harbinensis , Lactobacillus perolens , Lactobacillus < RTI ID = 0.0 > rhamnosus , Lactobacillus < RTI ID = 0.0 > vaccinostercus ), Acetobacter lovaniensis , Acetobacter peroxydans , Pichia < RTI ID = 0.0 > fermentans), it provides a Candida ethanol Rica (Candida ethanolica) and Saccharomyces Rommie Cobb Scoring System Ennis composition for removing heavy metals containing beneficial microorganism culture broth by culturing a microorganism useful mixture of (Saccharomycopsis schoenii) as an active ingredient.

In the heavy metal removal composition of the present invention, the useful microorganism is selected from the group consisting of Thiobacillus thiooxidans , Bacillus thuringiensis , Weissella < RTI ID = 0.0 > cibaria , bicellapramesenteroid ( Weissella paramesenteroides , and Bacillus amyloliquefaciens . < / RTI >

Further, in the composition for removing heavy metals of the present invention, the heavy metal may be at least one heavy metal selected from the group consisting of lead, chromium, copper, nickel and zinc, but is not limited thereto.

Hereinafter, embodiments of the present invention will be described in detail. However, the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

Comparative Example  1: Preparation of useful microbial culture

Lactobacillus < RTI ID = 0.0 > parafarraginis , Lactobacillus < RTI ID = 0.0 > paracasei , Lactobacillus tolerans , Lactobacillus < RTI ID = 0.0 > buchneri , Lactobacillus < RTI ID = 0.0 > harbinensis , Lactobacillus perolens , Lactobacillus < RTI ID = 0.0 > rhamnosus , Lactobacillus < RTI ID = 0.0 > vaccinostercus ), Acetobacter lovaniensis ), Acetobacter peroxydans , Pichia fermentans ), Candida ethanolica ( Candida ethanolica ) and Saccharomycopsis schoenii were mixed and cultured at 25 ° C for about 120 hours to prepare a culture solution of 1 × 10 ⁷ CFU / ml.

Manufacturing example  1: Preparation of useful microbial culture

Lactobacillus < RTI ID = 0.0 > parafarraginis , Lactobacillus < RTI ID = 0.0 > paracasei , Lactobacillus tolerans , Lactobacillus < RTI ID = 0.0 > buchneri , Lactobacillus < RTI ID = 0.0 > harbinensis , Lactobacillus perolens , Lactobacillus < RTI ID = 0.0 > rhamnosus , Lactobacillus < RTI ID = 0.0 > vaccinostercus ), Acetobacter lovaniensis ), Acetobacter peroxydans , Pichia fermentans ), Candida ethanolica ( Candida ethanolica ), Saccharomycopsis schoenii , thiobacillus thiooxidans), Bacillus Turing Gen System (Bacillus thuringiensis , Weissella cibaria), by Salah Farah mesen steroid (Weissella paramesenteroides) and Bacillus amyl Lori query Pacifico Enschede (Bacillus amyloliquefaciens ) were mixed and cultured at 25 ° C. for about 120 hours to prepare a culture solution of 1 × 10 ⁷ CFU / ml.

Manufacturing example  2: Fermentation using a useful microbial culture Safflower  Produce

(a) 20 liters of livestock manure were fermented at 25 to 37 ° C for 25 to 35 days while adding 20 liters of the prepared microbial culture solution of Preparation Example 1 and 15 kg of potato starch once a day, and the fermentation product was prepared by solid-liquid separation .

(b) To 20 tons of livestock manure, 20 L of the microbial culture solution of Preparation Example 1 and 15 kg of carbon source were added, followed by fermentation at 25 to 37 DEG C for 1 day. To this, 20 L of the microbial culture solution of Production Example 1 was added 20 L of the microbial culture prepared in Preparation Example 1, 2 tons of the fermented product prepared in the above step (a) and 15 kg of potato starch were added to the fermented product subjected to the first fermentation process, which was fermented for 5 days while being added once, The fermentation broth was prepared by repeating the first and second fermentation processes for 2 to 3 days at 25 to 37 ° C for 7 to 10 days while adding them once.

Example  1: Odor removal effect of livestock manure

The gas concentration of hydrogen sulfide (H ₂ S) was measured while fermenting at 30 ° C after mixing the microbial culture (1 × 10 ⁷ CFU / ml) with livestock manure at a ratio of 100: 3 (w: v). The types of useful microorganism cultures were those cultured with different microorganisms as shown in Table 1 below.

Type of useful microorganism culture Strain type Comparative Example 1 Comparative Example 2 Comparative Example 3 Production Example 1 Lactobacillus parafarraginis ○ ○ ○ ○ Lactobacillus paracasei ○ ○ ○ ○ Lactobacillus tolerance ○ ○ ○ ○ Lactobacillus buchneri ○ ○ ○ ○ Lactobacillus harbinensis ○ ○ ○ ○ Lactobacillus perolens ○ ○ ○ ○ Lactobacillus rhamnosus ○ ○ ○ ○ Lactobacillus vaccinostercus ○ ○ ○ ○ Acetobacter lovaniensis ○ ○ ○ ○ Acetobacter peroxydans ○ ○ ○ ○ Pichia fermentans ○ ○ ○ ○ Candida ethanolica ○ ○ ○ ○ Saccharomycopsis schoenii ○ ○ ○ ○ Thiobacillus thiooxidans - ○ ○ ○ Bacillus thuringiensis - ○ ○ ○ Weissella cibaria - - ○ ○ Weissella paramesenteroides - - - ○ Bacillus amyloliquefaciens - - ○ ○

As a result, as the fermentation period was longer than the hydrogen sulfide concentration of the initial livestock manure, the reduction rate of hydrogen sulfide tended to increase. In the livestock manure 30 days after fermentation using the useful microorganism culture of Preparation Example 1, Therefore, it was confirmed that the treatment with livestock manure using the useful microorganism culture of Preparation Example 1 was superior to the livestock manure removal of odor gas (Table 2).

Effect of removing odor gas from pig manure Fermentation period Type of useful microorganism culture Decrease of hydrogen sulfide (%)
10 days Comparative Example 1 60.1 Comparative Example 2 65.4 Comparative Example 3 59.6 Production Example 1 75.1
20 days Comparative Example 1 69.2 Comparative Example 2 67.5 Comparative Example 3 62.4 Production Example 1 97.3
30 days Comparative Example 1 76.9 Comparative Example 2 69.5 Comparative Example 3 64.5 Production Example 1 99.5

Example  2: Odor removal method in pig farm

As a result of Example 1, since the useful microorganism culture liquid has an effect of eliminating odor of livestock manure, it can be used to remove odor from livestock manure in livestock farming, and the usage method is as follows.

1) Oral administration of a diluted solution of the useful microbial culture (2.2 × 10 ⁷ CFU / ml) of Comparative Example 1 or Production Example 1 to 500-1000 mL / ton of drinking water to the pigs can reduce the odor from the excreted side .

2) 200 liters of useful microorganism culture per 600 m ^{2 of} pigs can be sprayed with mist spraying to remove odor in the air and evenly spread throughout the inner barrels to decompose the odor-causing substances in the pig's pests.

3) In order to remove the odor coming from the pig manure, diluted liquid of 60 kg of water and 15 kg of carbon source was sprayed on 20 tons of manure, and the useful microorganism culture was sprayed thereon. In this way, the odor was removed from the pesticide by treating it 3-5 times once every 2 days. The useful microorganism culture solution can be used by diluting the culture solution and water at a ratio of 1:10 to 20.

Example  3: Removal of heavy metals from livestock manure

The liquid fertilizer prepared by the method of Preparation Example 2 and the liquid fertilizer prepared by the method of Preparation Example 2 but using the useful microorganism culture of Comparative Example 1 of Comparative Example 1 without using the useful microorganism of Production Example 1 (Comparative Example 4) Table 3 shows the results of comparing the heavy metal content of the liquid fertilizer prepared using the useful microorganism culture of Comparative Example 2 (Comparative Example 5) and the liquid fertilizer prepared using the useful microorganism culture of Comparative Example 3 (Comparative Example 6) .

As a result, it was confirmed that the content of heavy metals such as chromium, copper, nickel and zinc decreased by the fermentation of useful microorganisms compared to livestock manure, and the use of the useful microorganisms of Preparation Example 1 It was confirmed that the production of the liquid (preparation example 2) was excellent in the heavy metal removal effect in the livestock manure.

Removal of Heavy Metals from Livestock Manure Inspection items Specification standard Livestock manure Comparative Example 4 Comparative Example 5 Comparative Example 6 Production Example 2 Arsenic (mg / kg) 5 or less Non-detection Non-detection Non-detection Non-detection Non-detection Cadmium (mg / kg) 0.5 or less Non-detection Non-detection Non-detection Non-detection Non-detection Mercury (mg / kg) 0.2 or less Non-detection Non-detection Non-detection Non-detection Non-detection Lead (mg / kg) 15 or less 0.17 Non-detection Non-detection Non-detection Non-detection Chromium (mg / kg) 30 or less 7.24 1.77 2.54 1.86 1.42 Copper (mg / kg) Less than 50 16.40 12.03 8.96 11.20 0.37 Nickel (mg / kg) 5 or less 4.48 1.15 1.02 1.68 0.69 Zinc (mg / kg) 130 or less 42.17 4.61 5.69 20.58 Non-detection

Example  4: Liquid  Effects of crop fertilization

1) Growth characteristics of Chinese cabbage

The liquid processing agent diluted 1/500 (v / v) was added to the liquid of Preparation Example 2, and about 4.05 L of the liquid processing agent diluted with 1/500 (v / v) was cultivated in the transplanted Chinese cabbage at intervals of 3 to 5 days. Comparative Example 7 was prepared by the method of Production Example 2, except that the cultivation of the useful microorganism of Comparative Example 1 was carried out without using the culture of the useful microorganism of Production Example 1, Which is cultivated using the Chinese cabbage.

The results of investigating the growth of Chinese cabbage according to the cultivation conditions of Chinese cabbage are shown in Table 4 below. The harvested Chinese cabbage was cultivated for 60 days after harvesting, and the cabbage cultivated using the safflower of Preparation Example 2 was compared with the control and Comparative Example 7, It was confirmed that the growth of leaf area and leaf width was improved in the medium.

Growth of Chinese cabbage according to the type of liquid division Production Example 2 Lactic acid Non-treatment Comparative Example 7 Weekday (g) 3,910 3,555 3,681 (G) 2,600 2,414 2,547 Leaf length (cm) 42.0 41.6 41.5 Leaf width (cm) 31.1 28.2 30.7 Number of leaves () 78.4 78.1 78.0

2) Fertility effect on pears

After the pear was harvested, the livestock of Preparation Example 2 was fertilized in the late fall around the perimeter of the pear tree about 20 cm, and the pears were harvested the next year, and the size and the sugar content were evaluated. The non-treatment means means a harvested harvest without using the liquid fertilizer. Comparative Example 8 was prepared by the method of Production Example 2, except that the cultivation of the useful microorganism culture of Comparative Example 1 without using the culture of the useful microorganism of Production Example 1, Means a ship harvested using.

As a result, it was confirmed that the boats cultivated using potting liquor were higher in size and higher in sugar content than the untreated pots, so that it was possible to harvest the superior quality livers with excellent color and shape. In particular, it was confirmed that when cultivated using the liquid fertilizer of Production Example 2, it exhibited high sugar content and quality, and it is expected that the fertilizer with improved sugar content and size can be produced by using the fertilizer of Production Example 2 of the present invention.

Quality characteristics of ship division Upper crop harvest
(Color, shape, etc.) Size of fruit
(600 g or more) Brix Non-treatment 46 kg / week 50% 10.8 Production Example 2 Lactic acid 52 kg /week 62% 12.5 Comparative Example 8 48 kg / week 59% 11.7

3) Rice cultivation effect using potting

In order to carry out the rice cultivation effect test using the liquid fertilizer, a certain amount of paddy soil was filled into a pot of a size of 44 cm (wide) × 58 cm (length) × 20 cm (height) and then seeded at a depth of 2 to 3 cm After planting the seeds of Nipyeongbyeo rice, four seeds of five seeds of rice were planted in two rows. Then the fertilizer of Preparation Example 2 was fertilized and rice was grown for 180 days. The non-treatment means means the rice harvested without using the liquid fertilizer, and Comparative Example 9 means the rice harvested using the liquid fertilizer produced using the useful microorganism culture of Comparative Example 1 without using the useful microbial culture liquid of Production Example 1 do.

As a result, it was possible to increase the yield of rice by cultivating rice using liquid fertilizer in comparison with non-fertilizer, and the rice bran cultivated with the fertilizer of Preparation Example 2 had a large number of effective fractions directly affecting rice yield The yield of rice was the highest, and it was confirmed that the liquid yield of Production Example 2 of the present invention can secure an excellent yield (Table 6).

Rice cultivation effect division Number of valid tries (pieces / week) Yield (kg / 10a) Non-treatment 8 485 Production Example 2 Lactic acid 18 610 Comparative Example 9 14 574

Claims (7)

delete delete delete delete delete Lactobacillus parafarrhinis , Lactobacillus paracasei , Lactobacillus tolerans , Lactobacillus buchneri , Lactobacillus harbinensis , Lactobacillus spp ., Lactobacillus spp ., Lactobacillus spp . Lactobacillus perolens , Lactobacillus rhamnosus , Lactobacillus vaccinostercus , Acetobacter lovaniensis , Acetobacter peroxydans , Pichia perianthus, Tansu (Pichia fermentans), Candida ethanol Rica (Candida ethanolica), Saccharomyces Rommie Cobb cis Scoring Enigma (Saccharomycopsis schoenii), thio Bacillus thio oxy residence (Thiobacillus thiooxidans), Bacillus Turing Gen system (Bacillus thuringiensis), Bar Sela cibaria (Weissella cibaria), By Selah parametric Centenario Id (Weissella paramesenteroides) and Bacillus amyl Lowry query Pacific Enschede (Bacillus amyloliquefaciens) useful for the beneficial microorganism cultured mixture of the microorganism culture solution containing chromium as an active component, copper, nickel, and heavy metals removal composition of the zinc. delete

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