CN107098739A - The preparation method for the livestock waste fermentation liquid fertilizer being reduced using the stench and content of beary metal of effective microbe - Google Patents
The preparation method for the livestock waste fermentation liquid fertilizer being reduced using the stench and content of beary metal of effective microbe Download PDFInfo
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- CN107098739A CN107098739A CN201710090108.8A CN201710090108A CN107098739A CN 107098739 A CN107098739 A CN 107098739A CN 201710090108 A CN201710090108 A CN 201710090108A CN 107098739 A CN107098739 A CN 107098739A
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- Prior art keywords
- lactobacillus
- effective microbe
- fermentation
- nutrient solution
- effective
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 101
- 238000000855 fermentation Methods 0.000 title claims abstract description 84
- 230000004151 fermentation Effects 0.000 title claims abstract description 84
- 239000007788 liquid Substances 0.000 title claims abstract description 84
- 238000002360 preparation method Methods 0.000 title claims abstract description 64
- 244000144972 livestock Species 0.000 title claims abstract description 47
- 239000002699 waste material Substances 0.000 title claims abstract description 47
- 229910052751 metal Inorganic materials 0.000 title claims abstract description 27
- 239000002184 metal Substances 0.000 title claims abstract description 27
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethanethiol Chemical compound CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 title abstract description 18
- 235000015097 nutrients Nutrition 0.000 claims abstract description 65
- 239000000203 mixture Substances 0.000 claims abstract description 26
- 238000000034 method Methods 0.000 claims abstract description 22
- 239000004480 active ingredient Substances 0.000 claims abstract description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 25
- 241000186660 Lactobacillus Species 0.000 claims description 25
- 229910052799 carbon Inorganic materials 0.000 claims description 25
- 229940039696 lactobacillus Drugs 0.000 claims description 25
- 241000589220 Acetobacter Species 0.000 claims description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 20
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims description 16
- 241000186605 Lactobacillus paracasei Species 0.000 claims description 15
- 241000751212 Lactobacillus vaccinostercus Species 0.000 claims description 14
- 239000001963 growth medium Substances 0.000 claims description 14
- 238000009629 microbiological culture Methods 0.000 claims description 14
- 238000000926 separation method Methods 0.000 claims description 14
- 239000007787 solid Substances 0.000 claims description 13
- 241000607142 Salmonella Species 0.000 claims description 12
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 10
- 241000235058 Komagataella pastoris Species 0.000 claims description 10
- 241000831743 Lactobacillus parafarraginis Species 0.000 claims description 10
- 241001448603 Lactobacillus perolens Species 0.000 claims description 10
- 241000178268 Saccharomycopsis schoenii Species 0.000 claims description 10
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims description 8
- 241000925032 Lactobacillus harbinensis Species 0.000 claims description 8
- 241001497699 Acetobacter peroxydans Species 0.000 claims description 7
- 241000186679 Lactobacillus buchneri Species 0.000 claims description 7
- 241000521553 Pichia fermentans Species 0.000 claims description 7
- 241000509461 [Candida] ethanolica Species 0.000 claims description 7
- 241001497546 Acetobacter lovaniensis Species 0.000 claims description 6
- 241000605118 Thiobacillus Species 0.000 claims description 6
- 241000605272 Acidithiobacillus thiooxidans Species 0.000 claims description 4
- 241000193388 Bacillus thuringiensis Species 0.000 claims description 4
- 241000975185 Weissella cibaria Species 0.000 claims description 4
- 229940097012 bacillus thuringiensis Drugs 0.000 claims description 4
- 208000002874 Acne Vulgaris Diseases 0.000 claims description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 2
- 239000005864 Sulphur Substances 0.000 claims description 2
- 241000192133 Weissella paramesenteroides Species 0.000 claims description 2
- 206010000496 acne Diseases 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 claims description 2
- 230000003647 oxidation Effects 0.000 claims 1
- 238000007254 oxidation reaction Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 19
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 9
- 239000002689 soil Substances 0.000 abstract description 8
- 239000003905 agrochemical Substances 0.000 abstract description 6
- 239000000126 substance Substances 0.000 abstract description 6
- 238000009264 composting Methods 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 3
- 239000007921 spray Substances 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 50
- 230000000052 comparative effect Effects 0.000 description 20
- 241000209094 Oryza Species 0.000 description 13
- 235000007164 Oryza sativa Nutrition 0.000 description 13
- 235000009566 rice Nutrition 0.000 description 13
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 10
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 10
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 10
- 238000003306 harvesting Methods 0.000 description 10
- 241000251468 Actinopterygii Species 0.000 description 8
- 206010059410 Faecaluria Diseases 0.000 description 8
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 8
- 241001465754 Metazoa Species 0.000 description 7
- 241000220324 Pyrus Species 0.000 description 7
- 235000021017 pears Nutrition 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 241000894007 species Species 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 4
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 4
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 4
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 4
- 229910052804 chromium Inorganic materials 0.000 description 4
- 239000011651 chromium Substances 0.000 description 4
- 229910052802 copper Inorganic materials 0.000 description 4
- 239000010949 copper Substances 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 210000003608 fece Anatomy 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 229910052759 nickel Inorganic materials 0.000 description 4
- 229910052725 zinc Inorganic materials 0.000 description 4
- 239000011701 zinc Substances 0.000 description 4
- 239000007789 gas Substances 0.000 description 3
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 3
- 239000011133 lead Substances 0.000 description 3
- 239000007791 liquid phase Substances 0.000 description 3
- 239000010871 livestock manure Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 240000001987 Pyrus communis Species 0.000 description 2
- 235000014443 Pyrus communis Nutrition 0.000 description 2
- 241000202221 Weissella Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000003895 organic fertilizer Substances 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- 241000272525 Anas platyrhynchos Species 0.000 description 1
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000006701 autoxidation reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910052796 boron Inorganic materials 0.000 description 1
- 229910052793 cadmium Inorganic materials 0.000 description 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 201000003740 cowpox Diseases 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 238000007726 management method Methods 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000009335 monocropping Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000009329 organic farming Methods 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- RMAQACBXLXPBSY-UHFFFAOYSA-N silicic acid Chemical compound O[Si](O)(O)O RMAQACBXLXPBSY-UHFFFAOYSA-N 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
It the present invention relates to the use of the preparation method for the fermentation liquid fertilizer that the content of beary metal of effective microbe is reduced；The fermentation liquid fertilizer that content of beary metal is reduced, it is characterised in that it utilizes the effective microbe prepared by the above method；And for the composition for the content of beary metal for reducing fermentation liquid fertilizer, it is characterised in that it contains effective microbe nutrient solution as active ingredient.The heavy metals removal excellent effect of the effective microbe nutrient solution of the present invention, therefore the effective microbe nutrient solution can effectively utilize for reducing the purpose of the heavy metal contained in soil and water.In addition, the present invention can provide instead of agricultural chemicals and chemical fertilizer the liquid fertilizer for being capable of crop culture in environmentally friendly manner, it has the characteristics that：The stench of livestock waste is effectively removed in Composting Process, so that any period in the four seasons can be diluted with water and spray, and the heavy metal contained in livestock waste can be effectively reduced.
Description
Technical field
It the present invention relates to the use of the livestock waste fermentation liquid fertilizer that the stench and content of beary metal of effective microbe are reduced
Preparation method；The fermentation liquid fertilizer that content of beary metal is reduced, it is characterised in that it utilizes having for being prepared by the above method
Imitate microorganism；And for the composition for the content of beary metal for reducing fermentation liquid fertilizer, it is characterised in that it contains effective microbe
Nutrient solution is used as active ingredient.
Background technology
Fertilizer is to bring the material of indirect help to growing for crop, the nutrients without directly forming crop
Matter, the indirect help increases effective microbe to improve the physical chemistry of soil, while will be unable to utilize in soil
Available form is converted in the nutrient of the form of plant, and reduces the toxicity of toxic material.
According to the fertilizer management method of current South Korea, this fertilizer is roughly divided into common fertilizer and special fertilizer, it is specific and
Speech, common fertilizer is divided into inanimate matter fertilizer (the inanimate matter nitrogen based on the key element of the principal component of fertilizer i.e. nitrogen, phosphoric acid, potassium etc. 3
Fertilizer, inanimate matter phosphatic manure, inanimate matter potassic fertilizer), compound fertilizer, organic fertilizer, calcium carbonate fertilizer, silicic acid matter fertilizer,
Magnesium fertilizer, boron fertilizer etc., special fertilizer are divided into various self-control fertilizer and accessory substance fertilizer including heap barnyard manure etc., but not
Classify according to specific benchmark, but arbitrary classification as needed.
In general, chemical fertilizer can make soil acidity, and can polluted underground water, therefore encourage organic agriculture just into
For trend.For this organic farming, the safe crops influenceed from agricultural chemicals are provided using the fecaluria of domestic animal, and
Effectively using a large amount of fecalurias produced in the animal house of domestic animal, so as to reduce various facilities and funds for fecaluria processing.
The livestock waste produced in the animal house of domestic animal can play the growth for promoting raise crop and the taste for improving fruit
With the effect of pol, even if but livestock waste is used as organic fertilizer in itself, good effect can not be expected, therefore
It is used as fertilizer after Composting preprocessing process.However, can be produced during Composting is carried out to livestock waste a lot
Stench, and when as Fertilizer application, the heavy metal contained in livestock waste may be accumulated in plant.
Korean granted patent the 1441005th discloses the preparation method that plant growth promotes liquid fertilizer, Korean granted patent
No. 1156650 discloses the preparation method for utilizing the sour liquid fertilizer for removing herding fecaluria stench of water soluble corrosion, however, these are all
The system for the livestock waste fermentation liquid fertilizer being reduced different from the stench and content of beary metal of the utilization effective microbe of the present invention
Preparation Method.
The content of the invention
The technical problem to be solved
The present invention proposes that the purpose of the present inventor is to provide the hair using effective microbe according to above-mentioned requirements
Zymotic fluid fertilizer preparation method and contain effective microbe nutrient solution as active ingredient, for reduce ferment liquid fertilizer heavy metal
The composition of content, wherein the effective microbe has following characteristics：, can not only when preparing liquid fertilizer using livestock waste
Effectively remove the stench produced by livestock waste, moreover it is possible to reduce the content of beary metal contained in fecaluria, and can effectively strengthen
The growth of plant.
Technical scheme
To achieve these goals, the present invention provides the zymotic fluid being reduced using the content of beary metal of effective microbe
The preparation method of fertilizer, it is characterised in that the preparation method comprises the following steps：(a) cultivate effective microbe and prepare effectively
Microbial culture medium；(b) the effective microbe nutrient solution prepared in the step (a) and carbon source are added in livestock waste, so
Fermented afterwards and implement separation of solid and liquid, so as to obtain fermentate；And (c) is added in livestock waste in the step (a)
Fermented after the effective microbe nutrient solution and carbon source of preparation, preparation in the step (a) is then further added wherein
Effective microbe nutrient solution carry out one time fermentation, add the step in the fermentate Jing Guo the one time fermentation process
(a) effective microbe nutrient solution, the fermentate of the separation of solid and liquid of the step (b) and the carbon source prepared in carries out secondary fermentation.
In addition, the present invention provides the fermentation liquid fertilizer that content of beary metal is reduced, it is characterised in that it is using by above-mentioned
Effective microbe prepared by method.
In addition, the composition of the content of beary metal the present invention is provided to reduce fermentation liquid fertilizer, it is characterised in that it contains
Effective microbe nutrient solution is used as active ingredient.
Beneficial effect
The heavy metals removal excellent effect of the effective microbe nutrient solution of the present invention, therefore the effective microbe nutrient solution
Can effectively it be utilized for reducing the purpose of the heavy metal contained in soil and water.
In addition, it is existing livestock waste is fermented and be used as fertilizer in the case of, only fermentation 2 years after can just be sprayed on
On crops, but fermented in the present invention using the species of specific effective microbe, so as to which fermentation time is contracted
It is short within 45 days.
In addition, the present invention can provide instead of agricultural chemicals and chemical fertilizer the liquid for being capable of crop culture in environmentally friendly manner
Fertilizer, it has the characteristics that：The stench of livestock waste is effectively removed in Composting Process, so that any period in the four seasons
It can be diluted with water and spray, and can effectively reduce the heavy metal contained in livestock waste.
Brief description of the drawings
Fig. 1 is the figure for the assay report for showing the fermentation liquid fertilizer of the present invention.
Embodiment
In order to realize the purpose of the present invention, the present invention provides the hair being reduced using the content of beary metal of effective microbe
The preparation method of zymotic fluid fertilizer, it is characterised in that the preparation method comprises the following steps：
(a) culture effective microbe mixture and prepare effective microbial culture medium, the effective microbe mixture by
Lactobacillus parafarraginis, lactobacillus paracasei (Lactobacillus pa racasei),
Lactobacillus tolerans, Bu Shi lactobacillus (Lactobacillus buchneri), L actobacillus
Harbinensis, Lactobacillus perolens, Lactobacillus rhamnosus (Lactobac illus rhamnosus), ox
Acne lactobacillus (Lactobacillus vaccinostercus), Luo Wang acetobacters (Acetobacter lovaniensis), mistake
Aoxidize the false silk ferment of acetobacter (Acetobacter peroxydans), fermentation Pichia pastoris (Pichia fermentans), ethanol
Female (Candida ethanolica) and Sacc haromycopsis schoenii compositions；
(b) the effective microbe nutrient solution prepared in the step (a) and carbon source are added in livestock waste, is then carried out
Ferment and implement separation of solid and liquid, so as to obtain fermentate；And
(c) the effective microbe nutrient solution prepared in the step (a) is added in livestock waste and is sent out after carbon source
Ferment, then further adds the effective microbe nutrient solution prepared in the step (a) and carries out one time fermentation, passing through wherein
The effective microbe nutrient solution prepared in the step (a), the step (b) are added in the fermentate of the one time fermentation process
Separation of solid and liquid fermentate and carbon source carry out secondary fermentation.
In the preparation method of the fermentation liquid fertilizer of the present invention, the carbon source is preferably farina, compared with other carbon sources,
The farina has advantages below, i.e., unit price is cheap, and cultivates the excellent effect of the effective microbe.
In addition, the present invention fermentation liquid fertilizer preparation method in, the livestock waste be preferably selected from pig, ox, chicken, duck,
The fecaluria of the domestic animal of one or more of horse and dog, but it is not limited to this.
In addition, in the preparation method of the fermentation liquid fertilizer of the present invention, the feature of fermentation liquid fertilizer of the invention is content of beary metal
It is reduced, the heavy metal can be lead, chromium, the heavy metal of one or more of copper, nickel and zinc, but be not limited to this.
The preparation method of the fermentation liquid fertilizer of the present invention more specifically comprises the following steps：
(a) cultivated 92~140 hours at a temperature of 23~35 DEG C after mixing effective microbe, so as to prepare 1 × 106~ 8CFU/ml(CFU：CFU) concentration effective microbe nutrient solution, the microorganism is by Lactobacillus
Parafarraginis, lactobacillus paracasei (Lactobacillus para casei), Lactobacillus tolerans,
Bu Shi lactobacillus (Lactobacillus buchneri), Lact obacillus harbinensis, Lactobacillus
Perolens, Lactobacillus rhamnosus (Lactobacill us rhamnosus), Lactobacillus vaccinostercus (Lactobacillus
Vaccinostercus), Luo Wang acetobacters (A cetobacter lovaniensis), peroxidating acetobacter (Acetobacter
Peroxydans), fermentation Pichia pastoris (Pichia fermentans), ethanol Candida (Candida ethanolica)
And Sacchar omycopsis schoenii compositions；
(b) the effective microbe nutrient solution prepared in the step (a) and carbon source are added in livestock waste, then 25
Fermentation 25~35 days is carried out at a temperature of~37 DEG C and implements separation of solid and liquid, so as to obtain fermentate；And
(c) the effective microbe training prepared in the step (a) that 16~24L is added in 15~25 tons of livestock waste
Fermentation 1~2 day is carried out after nutrient solution and 12~18kg carbon source at a temperature of 25~37 DEG C, then with every 1~2 day frequency once
Rate carries out one time fermentation 4~6 days while further adding the 16~24L effective microbe nutrient solution wherein, in warp
Cross in the fermentate of the one time fermentation process accurate in the step (a) that 16~24L is added with every 1~2 day frequency once
Standby effective microbe nutrient solution, the fermentate and 12~18kg carbon of the separation of solid and liquid of 1.6~2.4 tons of the step (b)
Source, while carry out secondary fermentation 7~10 days at a temperature of 25~37 DEG C, repeat described in once and secondary fermentation process 2~3
It is secondary.
More specifically, comprise the following steps：
(a) cultivated 120 hours at a temperature of 25 DEG C after mixing effective microbe, so as to prepare 1 × 107CFU/ml concentration
Effective microbe nutrient solution, the microorganism is by Lactobacillus parafarraginis, lactobacillus paracasei
(Lactobacillus paracasei), Lactobacillus tolerans, Bu Shi lactobacillus (Lactobacillus
Buchneri), Lactobacillus harbinensis, Lactobacillus perolens, Lactobacillus rhamnosus
(Lactobacillus rhamnosus), Lactobacillus vaccinostercus (Lactobacillus vaccinostercus), Luo Wang acetobacters
(Acetobacter lovaniensis), peroxidating acetobacter (Acetobacter peroxydans), fermentation Pichia pastoris
(Pichia fermentans), ethanol Candida (Candida ethanolica) and Saccharomycopsis
Schoenii is constituted；
(b) the effective microbe nutrient solution prepared in the step (a) and carbon source are added in livestock waste, then 25
Fermentation 25~35 days is carried out at a temperature of~37 DEG C and implements separation of solid and liquid, so as to obtain fermentate；And
(c) in 20 tons of livestock waste add 20L the step (a) in prepare effective microbe nutrient solution and
Fermentation 1 day is carried out after 15kg carbon source at a temperature of 25~37 DEG C, is then further added wherein with frequency once a day
Plus 20L the effective microbe nutrient solution while carry out one time fermentation 5 days, in the fermentation Jing Guo the one time fermentation process
The effective microbe nutrient solution of preparation, 2 tons of the step in the 20L step (a) are added in thing with frequency once a day
Suddenly the fermentate of the separation of solid and liquid of (b) and 15kg carbon source, while carrying out secondary fermentation 7~10 at a temperature of 25~37 DEG C
My god, repeat described in once and secondary fermentation process 2~3 times.
In the preparation method of the fermentation liquid fertilizer of the present invention, the effective microbe of the step (a) can be further comprising choosing
Autoxidation sulphur Thiobacillus (Thiobacillus thiooxidans), thuringiensis (Bacillus
Thuringiensis), cibarium Wei Si Salmonellas (Weissella cibaria), class goldbeater's skin Wei Si Salmonellas (Weissella
) and one or more of bacillus amyloliquefaciens (Bacillus amyloliquefaciens) paramesenteroides
Effective microbe, can not only be more effective when the effective microbe nutrient solution is added in livestock waste and is fermented
Ground removes the stench and heavy metal of livestock waste, and can in a short time ferment and livestock waste and be prepared into liquid fertilizer product.
Contain effective microbe in the fermentation liquid fertilizer of the present invention, therefore, it is possible to show the function of fertilizer and the work(of agricultural chemicals simultaneously
Energy.That is, by liquid fertilizer be used for crops cultivation when, due to the fermentation of effective microbe, can not only supply sufficient nutrition into
Point, but also the effect on conditioned soil ground can be obtained, especially, by antagonistic microbe can obtain cover crop from disease or
The effect of germ influence, thus it is more effective to the soil damaged by continuous cropping.Therefore, when the liquid fertilizer using the present invention, even if
The agricultural chemicals for not using chemical method to prepare can also protect crops from the influence of disease or disease pest, so as to can not only carry
High harvest yield, but also the crops of high-quality can be cultivated and consumer is supplied.Especially, even if not using chemical method system
Standby agricultural chemicals can also protect crops from the influence of disease or disease pest, therefore, it is possible to prevent the pollution of the environment, and can prevent
Ecosystem is destroyed.
In addition, the present invention provides the fermentation liquid fertilizer that content of beary metal is reduced, it is characterised in that it is using by above-mentioned
Effective microbe prepared by method.The fermentation liquid fertilizer can be used according to method commonly used in the art.
In addition, the composition of the content of beary metal the present invention is provided to reduce fermentation liquid fertilizer, it is characterised in that it contains
Effective microbe nutrient solution is as active ingredient obtained from cultivating effective microbe mixture, the effective microbe mixture
By Lactobacillus parafarraginis, lactobacillus paracasei (Lactobacillus paracasei),
Lactobacillus tolerans, Bu Shi lactobacillus (Lactobacillus buchneri), Lactobacillus
Harbinensis, Lactobacillus perolens, Lactobacillus rhamnosus (Lactobacillus rhamnosus), cowpox
Lactobacillus (Lactobacillus vaccinostercus), Luo Wang acetobacters (Acetobacter lovaniensis), peroxide
Change acetobacter (Acetobacter peroxydans), fermentation Pichia pastoris (Pichia fermentans), ethanol Candida
(Candida ethanolica) and Saccharomycopsis schoenii are constituted.
The present invention's is used in the composition of the content of beary metal of reduction fermentation liquid fertilizer, and the effective microbe can enter one
Step includes Thiobacillus thioxidans (Thiobacillus thiooxidans), thuringiensis (Bacillus
Thuringiensis), cibarium Wei Si Salmonellas (Weissella cibaria), class goldbeater's skin Wei Si Salmonellas (Weissella
Paramesenteroides) and bacillus amyloliquefaciens (Bacillus amyloliquefaciens), but it is not limited to
This.
In addition, the present invention's is used in the composition of the content of beary metal of reduction fermentation liquid fertilizer, the heavy metal can be
Heavy metal selected from one or more of lead, chromium, copper, nickel and zinc, but it is not limited to this.
Below, embodiments in accordance with the present invention are described in detail.But, following embodiments are only used for illustrating the present invention,
Present disclosure is not exposed to the limitation of following embodiments.
Comparative example 1：The preparation of effective microbe nutrient solution
Cultivated after mixing effective microbe at a temperature of 25 DEG C about 120 hours, so as to prepare 1 × 107CFU/ml concentration
Microbial culture medium, the effective microbe be Lactobacillus parafarraginis, lactobacillus paracasei
(Lactobacillus paracasei), Lactobacillus tolerans, Bu Shi lactobacillus (Lactobacillus
Buchneri), Lactobacillus harbinensis, Lactobacillus perolens, Lactobacillus rhamnosus
(Lactobacillus rhamnosus), Lactobacillus vaccinostercus (Lactobacillus vaccinostercus), Luo Wang acetobacters
(Acetobacter lovaniensis), peroxidating acetobacter (Acetobacter peroxydans), fermentation Pichia pastoris
(Pichia fermentans), ethanol Candida (Candida ethanolica) and Saccharomycopsis
schoenii。
Preparation example 1：The preparation of effective microbe nutrient solution
Cultivated after mixing effective microbe at a temperature of 25 DEG C about 120 hours, so as to prepare 1 × 107CFU/ml concentration
Microbial culture medium, the effective microbe be Lactobacillus parafarraginis, lactobacillus paracasei
(Lactobacillus paracasei), Lactobacillus tolerans, Bu Shi lactobacillus (Lactobacillus
Buchneri), Lactobacillus harbinensis, Lactobacillus perolens, Lactobacillus rhamnosus
(Lactobacillus rhamnosus), Lactobacillus vaccinostercus (Lactobacillus vaccinostercus), Luo Wang acetobacters
(Acetobacter lovaniensis), peroxidating acetobacter (Acetobacter peroxydans), fermentation Pichia pastoris
(Pichia fermentans), ethanol Candida (Candida ethanolica), Saccharomycopsis
Schoenii, Thiobacillus thioxidans (Thiobacillus thiooxidans), thuringiensis (Bacillus
Thuringiensis), cibarium Wei Si Salmonellas (Weissella cibaria), class goldbeater's skin Wei Si Salmonellas (Weissella
) and bacillus amyloliquefaciens (Bacillus amyloliquefaciens) paramesenteroides.
Preparation example 2：Utilize the preparation of the fermentation liquid fertilizer of effective microbe nutrient solution
(a) the micro- life prepared in the 20L preparation example 1 is added with frequency once a day in 20 tons of livestock waste
The farina of thing nutrient solution and 15kg, while carrying out fermentation 25~35 days at a temperature of 25~37 DEG C and implementing solid-liquid point
From so that fermentate is made.
(b) after the microbial culture medium of the preparation example 1 and 15kg carbon source that 20L is added in 20 tons of livestock waste
Fermentation 1 day is carried out at a temperature of 25~37 DEG C, the 20L preparation example 1 is then added wherein with frequency once a day
Microbial culture medium while carry out one time fermentation 5 days, with daily one in the fermentate Jing Guo the one time fermentation process
Secondary frequency adds the fermentation prepared in the microbial culture medium prepared in the 20L preparation example 1,2 tons of the step (a)
The farina of thing and 15kg, while carry out secondary fermentation 7~10 days at a temperature of 25~37 DEG C, repeat described in once and
Secondary fermentation process 2~3 times, so that fermentation liquid fertilizer is made.
Embodiment 1：The odor destruction effect of livestock waste
With 100 in livestock waste:3(w:V) ratio mixes effective microbial culture medium (1 × 107CFU/ml), then
Fermented at a temperature of 30 DEG C, while determining hydrogen sulfide (H2S) gas concentration.It is as shown in table 1 below, use change micro-
Biological species and the species for the effective microbe nutrient solution cultivated.
Table 1
The species of effective microbe nutrient solution
Its result is shown, compared with the concentration of hydrogen sulfide of the livestock waste at initial stage, when the slip of hydrogen sulfide is with fermentation
Between it is elongated be in increase trend, it can be seen that utilize effective microbe nutrient solution, the fermentation livestock waste of the 30th day of preparation example 1
In almost without concentration of hydrogen sulfide, therefore, it can confirm the effective microbe nutrient solution using preparation example 1 to handle an animal manure
During urine, in terms of the effect of foul gas of livestock waste is removed more excellent (table 2).
Table 2
The removal effect of the foul gas of Pig dung and urine
Embodiment 2：The method for removing the stench in pig farm
The result of embodiment 1 is, because effective microbe nutrient solution has the effect for the stench for removing livestock waste, therefore
The effective microbe nutrient solution can be used in order to remove the stench of livestock waste in herding farmers', the following institute of application method
Show.
1) with drinking water per ton to 500~1000mL comparative example 1 or the effective microbe nutrient solution of preparation example 1 (2.2 ×
107CFU/ml) it is diluted and obtains dilution, the dilution is then orally administered to pig, so as to pig after reducing
The stench of the stool of excretion.
2) to every 600m of pig house2200L effective microbe nutrient solution is sprayed in the way of atomized spray, so as to remove
Stench in air, and whole internal affairs room is uniformly sprayed, so as to decompose the stench reason material in pig house.
3) in order to remove the stench produced by the fecaluria in pig house, the carbon source that 15kg is diluted in 60L water is obtained
Dilution be sprayed on 20 tons of fecaluria, effective microbial culture medium is then sprayed in the above.By this method, with two
Its frequency coprocessing 3~5 times once, so as to eliminate the stench in pig house., can be with for effective microbe nutrient solution
1:10~20 dilution proportion nutrient solution and water are simultaneously used.
Embodiment 3：Heavy metals removal effect in livestock waste
To prepared by the method for preparation example 2 liquid fertilizer, prepared by the method for preparation example 2 but preparation example 1 be not used
Effective microbe but prepared using the effective microbe nutrient solution of the comparative example 1 of table 1 liquid fertilizer (comparative example 4), using than
Liquid fertilizer (comparative example 5) compared with the effective microbe nutrient solution preparation of example 2, the effective microbe nutrient solution preparation using comparative example 3
Liquid fertilizer (comparative example 6) the result that is compared of content of beary metal as described in Table 3.
Its result can confirm that, compared with livestock waste, chromium, copper, the nickel of liquid fertilizer for fermenting effective microbe and preparing
And the content of the heavy metal such as zinc is reduced, and compared with the liquid fertilizer of comparative example, utilizes the effective microbe preparation solution of preparation example 1
When fertile (preparation example 2), the excellent effect in terms of the heavy metal in livestock waste is removed.
Table 3
Heavy metals removal effect in livestock waste
| Inspection project | Specification benchmark | Livestock waste | Comparative example 4 | Comparative example 5 | Comparative example 6 | Preparation example 2 |
| Arsenic (mg/kg) | Less than 5 | Do not detect | Do not detect | Do not detect | Do not detect | Do not detect |
| Cadmium (mg/kg) | Less than 0.5 | Do not detect | Do not detect | Do not detect | Do not detect | Do not detect |
| Mercury (mg/kg) | Less than 0.2 | Do not detect | Do not detect | Do not detect | Do not detect | Do not detect |
| Lead (mg/kg) | Less than 15 | 0.17 | Do not detect | Do not detect | Do not detect | Do not detect |
| Chromium (mg/kg) | Less than 30 | 7.24 | 1.77 | 2.54 | 1.86 | 1.42 |
| Copper (mg/kg) | Less than 50 | 16.40 | 12.03 | 8.96 | 11.20 | 0.37 |
| Nickel (mg/kg) | Less than 5 | 4.48 | 1.15 | 1.02 | 1.68 | 0.69 |
| Zinc (mg/kg) | Less than 130 | 42.17 | 4.61 | 5.69 | 20.58 | Do not detect |
Embodiment 4：Utilize the Crops in Applying Fertilizer effect of liquid fertilizer
1) characteristic of growing of Chinese cabbage
Chinese cabbage has been cultivated by the following method：Water is added in the liquid fertilizer of preparation example 2 and dilute with 1/500 (v/v) ratio
Release and obtain liquid phase treatment, battalion was irrigated to the Chinese cabbage for being implanted in Tanaka with the interval of 3~5 days with the liquid phase treatment
The processing of nutrient solution, per unit area is handled with the about 4.05L liquid phase treatment.Untreated fish group represents that liquid is not used
The Chinese cabbage of fertilizer cultivation, comparative example 7 represents that the effective microbe of preparation example 1 is prepared but be not used using the method by preparation example 2
Nutrient solution but the liquid fertilizer that is prepared using the effective microbe nutrient solution of comparative example 1 are come the Chinese cabbage cultivated.
According to the cultivation condition of Chinese cabbage, the result investigated is grown as described in Table 4 to Chinese cabbage.By Chinese cabbage
It is transplanted to behind field and was grown by 60 days, then harvested, and plant weight, balling weight, leaf length, leaf width and the number of sheets is carried out
The result of investigation, can confirm that compared with untreated fish group and comparative example 7, the Chinese cabbage cultivated using the liquid fertilizer of preparation example 2
Plant weight, balling weight, leaf length and growing for leaf width are strengthened.
Table 4
According to the survey of growth and development of the Chinese cabbage of liquid fertilizer species
| Distinguish | The liquid fertilizer of preparation example 2 | Untreated fish group | Comparative example 7 |
| Plant weight (g) | 3,910 | 3,555 | 3,681 |
| Balling weight (g) | 2,600 | 2,414 | 2,547 |
| Leaf length (cm) | 42.0 | 41.6 | 41.5 |
| Leaf width (cm) | 31.1 | 28.2 | 30.7 |
| The number of sheets (individual) | 78.4 | 78.1 | 78.0 |
2) to the fertilization effect of pear tree
Harvest after pears, applied at late fall with the liquid fertilizer of preparation example 2 in the bottom of pear tree with the girth of about 20cm radiuses
Fertilizer, then Second Year harvest pears and evaluate its size and pol result it is as shown in table 5.Untreated fish group represents that liquid fertilizer is not used
And the pears harvested, comparative example 8 represents to train using preparing by the method for preparation example 2 but the effective microbe of preparation example 1 is not used
Nutrient solution but the liquid fertilizer that is prepared using the effective microbe nutrient solution of comparative example 1 are come the pears that harvest.
Its result can confirm that, compared with untreated fish group, and the size for the pears cultivated using liquid fertilizer is also big, pol
Height, gloss and shape are also excellent, so as to harvest the pears of excellent quality.Especially, it can confirm that the liquid fertilizer using preparation example 2
To show high pol and quality in the case of cultivating, so as to expect to produce using the liquid fertilizer of the preparation example 2 of the present invention
Pol and size obtain enhanced fruit.
Table 5
The quality characteristic of pears
3) the rice cultivation effect of liquid fertilizer is utilized
In order to which the rice cultivation effect implemented using liquid fertilizer is tested, in 44cm (width) × 58cm (length) × 20cm (height) size
Flowerpot in fill the paddy soils of specified quantitative, then with 2~3cm depth formation seed furrow, then by Nan Ping rice
(Nampyeongbyeo) seed rice is seeded at 4 and often place's sowing 5, and it is two rows to plant.Then entered with the liquid fertilizer of preparation example 2
Row applies fertilizer and cultivates rice 180 days.The rice that untreated fish group represents unused liquid fertilizer and harvested, comparative example 9 represents that system is not used
The effective microbe nutrient solution of standby example 1 but the liquid fertilizer that is prepared using the effective microbe nutrient solution of comparative example 1 are harvested
Rice.
Its result can confirm that, compared with untreated fish group, and rice is cultivated using liquid fertilizer can improve the harvest of rice
Amount, wherein the harvest yield to rice in the rice cultivated using the liquid fertilizer of preparation example 2 brings the effective tillering directly affected
Number is more, so that highest rice harvest yield is shown, therefore the liquid fertilizer of the preparation example 2 of the present invention is able to ensure that excellent harvest
Measure (table 6).
Table 6
Rice cultivation effect
| Distinguish | Available tillering (individual/strain) | Harvest yield (kg/10a) |
| Untreated fish group | 8 | 485 |
| The liquid fertilizer of preparation example 2 | 18 | 610 |
| Comparative example 9 | 14 | 574 |
Claims (7)
1. the preparation method for the fermentation liquid fertilizer being reduced using the content of beary metal of effective microbe, it is characterised in that described
Preparation method comprises the following steps：
(a) culture effective microbe mixture and prepare effective microbial culture medium, the effective microbe mixture by
Lactobacillus parafarraginis, lactobacillus paracasei (Lactobacillus pa racasei),
Lactobacillus tolerans, Bu Shi lactobacillus (Lactobacillus buchneri), L actobacillus
Harbinensis, Lactobacillus perolens, Lactobacillus rhamnosus (Lactobac illus rhamnosus), ox
Acne lactobacillus (Lactobacillus vaccinostercus), Luo Wang acetobacters (Acetobacter lovaniensis), mistake
Aoxidize the false silk ferment of acetobacter (Acetobacter peroxydans), fermentation Pichia pastoris (Pichia fermentans), ethanol
Female (Candida ethanolica) and Sacc haromycopsis schoenii compositions；
(b) the effective microbe nutrient solution prepared in the step (a) and carbon source are added in livestock waste, is then fermented
And implement separation of solid and liquid, so as to obtain fermentate；And
(c) the effective microbe nutrient solution prepared in the step (a) is added in livestock waste and is fermented after carbon source, so
Further add the effective microbe nutrient solution prepared in the step (a) wherein afterwards and carry out one time fermentation, by described
The effective microbe nutrient solution for preparing in the step (a), the step (b) is added in the fermentate of one time fermentation process to consolidate
The fermentate and carbon source of liquid separation carry out secondary fermentation.
2. the preparation side for the fermentation liquid fertilizer that the content of beary metal of utilization effective microbe according to claim 1 is reduced
Method, it is characterised in that the effective microbe of the step (a) further includes Thiobacillus thioxidans (Thiobacillus
Thiooxidans), thuringiensis (Bacillus thur ingiensis), cibarium Wei Si Salmonellas (Weissella
Cibaria), class goldbeater's skin Wei Si Salmonellas (Weissella paramesenteroides) and bacillus amyloliquefaciens (Bacillus
amyloliquefaciens)。
3. the preparation side for the fermentation liquid fertilizer that the content of beary metal of utilization effective microbe according to claim 1 is reduced
Method, it is characterised in that the preparation method comprises the following steps：
(a) culture effective microbe mixture and prepare effective microbial culture medium, the effective microbe mixture by
Lactobacillus parafarraginis, lactobacillus paracasei, Lactobacillus t olerans, Bu Shi lactobacillus,
Lactobacillus harbinensis, Lactobacillus perolens, Lactobacillus rhamnosus, Lactobacillus vaccinostercus, Luo Wang
Acetobacter, peroxidating acetobacter, fermentation Pichia pastoris, ethanol Candida and Saccharomycopsis schoenii compositions；
(b) the effective microbe nutrient solution prepared in the step (a) and carbon source are added in livestock waste, is then fermented
And implement separation of solid and liquid, so as to obtain fermentate；And
(c) the effective microbe nutrient solution prepared in the step (a) that 16~24L is added in 15~25 tons of livestock waste
Fermented with after 12~18kg carbon source, what is then prepared wherein in 16~24L of the further addition step (a) has
One time fermentation is carried out while imitating microbial culture medium 4~6 days, and 16 are added in the fermentate Jing Guo the one time fermentation process
The effective microbe nutrient solution of preparation, the separation of solid and liquid of 1.6~2.4 tons of the step (b) in~24L step (a)
Fermentate and 12~18kg carbon source while carry out secondary fermentation 7~10 days, repeat described in once and secondary fermentation process 2
~3 times.
4. the preparation side for the fermentation liquid fertilizer that the content of beary metal of utilization effective microbe according to claim 2 is reduced
Method, it is characterised in that the preparation method comprises the following steps：
(a) culture effective microbe mixture and prepare effective microbial culture medium, the effective microbe mixture by
Lactobacillus parafarraginis, lactobacillus paracasei, Lactobacillus t olerans, Bu Shi lactobacillus,
Lactobacillus harbinensis, Lactobacillus perolens, Lactobacillus rhamnosus, Lactobacillus vaccinostercus, Luo Wang
Acetobacter, peroxidating acetobacter, fermentation Pichia pastoris, ethanol Candida, Saccharomycopsis schoenii, oxidation
Sulphur Thiobacillus, thuringiensis, cibarium Wei Si Salmonellas, class goldbeater's skin Wei Si Salmonellas and bacillus amyloliquefaciens composition；
(b) the effective microbe nutrient solution prepared in the step (a) and carbon source are added in livestock waste, is then fermented
And implement separation of solid and liquid, so as to obtain fermentate；And
(c) the effective microbe nutrient solution prepared in the step (a) that 16~24L is added in 15~25 tons of livestock waste
Fermented with after 12~18kg carbon source, what is then prepared wherein in 16~24L of the further addition step (a) has
One time fermentation is carried out while imitating microbial culture medium 4~6 days, and 16 are added in the fermentate Jing Guo the one time fermentation process
The effective microbe nutrient solution of preparation, the separation of solid and liquid of 1.6~2.4 tons of the step (b) in~24L step (a)
Fermentate and 12~18kg carbon source while carry out secondary fermentation 7~10 days, repeat described in once and secondary fermentation process 2
~3 times.
5. the fermentation liquid fertilizer that content of beary metal is reduced, it is characterised in that it is utilized by any one of Claims 1 to 4
Effective microbe prepared by described method.
6. the composition of the content of beary metal for reducing fermentation liquid fertilizer, it is characterised in that it contains culture effective microbe and mixed
Effective microbe nutrient solution obtained from compound is as active ingredient, and the effective microbe mixture is by Lactobacillus
Parafarraginis, lactobacillus paracasei, Lacto bacillus tolerans, Bu Shi lactobacillus, Lactobacillus
Harbinensis, Lactobacillus perolens, Lactobacillus rhamnosus, Lactobacillus vaccinostercus, Luo Wang acetobacters, peroxidating
Acetobacter, fermentation Pichia pastoris, ethanol Candida and Saccharomycopsis schoenii compositions.
7. the composition according to claim 6 for being used to reduce the content of beary metal of fermentation liquid fertilizer, it is characterised in that described
Effective microbe further comprising Thiobacillus thioxidans, thuringiensis, cibarium Wei Si Salmonellas, class goldbeater's skin Wei Si Salmonellas and
Bacillus amyloliquefaciens.
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| WO2023217056A1 (en) * | 2022-05-13 | 2023-11-16 | 青岛蔚蓝赛德生物科技有限公司 | Composite microbial deodorant, method for preparing same, and use thereof |
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| CN107098739B (en) | 2020-09-01 |
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