CN106635905A - Ecological restoration preparation for degraded soil obtained after cultivation of greenhouse vegetables, and preparation method of ecological restoration preparation - Google Patents
Ecological restoration preparation for degraded soil obtained after cultivation of greenhouse vegetables, and preparation method of ecological restoration preparation Download PDFInfo
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- CN106635905A CN106635905A CN201611229358.7A CN201611229358A CN106635905A CN 106635905 A CN106635905 A CN 106635905A CN 201611229358 A CN201611229358 A CN 201611229358A CN 106635905 A CN106635905 A CN 106635905A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C05—FERTILISERS; MANUFACTURE THEREOF
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- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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Abstract
The invention discloses an ecological restoration preparation for degraded soil obtained after cultivation of greenhouse vegetables, and a preparation method of the ecological restoration preparation. The ecological restoration preparation is prepared from bacillus subtilis, candida utilis, lactobacillus plantarum, lactobacillus casei, trichoderma viride and rhodopseudomonas palustris. The ecological restoration preparation starts from restoring the soil ecosystem, and increases the beneficial bacteria in the soil by using a microbial technology; furthermore, by adopting a multi-strain combination technical method, the ecological restoration preparation can promote the growth of plant roots while restoring the soil ecosystem; the ecological restoration preparation can loosen the soil and enable seedlings to be strong, and has a function of degrading the residual pollution of pesticides and chemical fertilizers.
Description
Technical field
The present invention relates to after a kind of microorganism formulation, more particularly to a kind of planting vegetable in greenhouse degenerated soil restoration of the ecosystem
Preparation and preparation method thereof.
Background technology
Booth vegetable cultivation is a kind of cultural method particularly important in the production of cold district vegetables, with good economy
Benefit and social benefit.But the current blindness due to the highly intensive closed management of green house of vegetables and chemical fertilizer excessively puts into,
Soil degradation problem is very prominent.
Long-term excessive fertilization, either chemical fertilizer or fertilizer can all cause salinity to accumulate in upper soll layer, occur secondary
Salination.When soil salt content is higher than 0.3% crop, particularly seedlings root will be suppressed to absorb nutrient.
The mainly a large amount of nitrogen fertilizer applications partially of soil acidification are caused, and it is all adversely affected to crop root and edaphon.
To pursue high benefit, long-term continuous cropping, makes soil microflora unbalance, and Soil Biodiversity is destroyed, and causes
The soil-borne diseases such as root-knot nematode, droop, epidemic disease.This single cropping such as " cucumber village ", " garlic township " area it is more common,
It is even more serious.
Suppression harmful bacteria is accumulated and is referred under long-term Continuous Mono-cropping in soil, the chemistry of crop root secretion, decomposition and leaching
Material to itself, the inhibitory action that produces of of the same race or kindred plant.It is a kind of allelopathy, soybean, tomato, eggplant, watermelon,
Auto toxicity is all easily produced during the continuous cropping such as muskmelon and cucumber.
A kind of field of vegetables soil is described in a kind of vegetable land soil amendment of patent CN200710175706 and preparation method thereof
Earth modifying agent, it include by weight by cow dung, wheat bran, brown coal, calcium nitrate, magnesium sulfate, borax, zinc sulfate, ferrous sulfate and
Water mixes, and composting fully ferments, and tunning adds a certain amount of bacillus subtilis microbial agent and is obtained.But due to being nature
Fermentation reactor system, the time of fermentation and effect are all relatively difficult to control, while the effect for improving soil is not also excellent.
The content of the invention
The present invention for solve that the problems referred to above of the prior art propose it is a kind of being capable of the effective rehabilitating soil ecosystem
Promote the growth of plant stock, strong sprout of loosening the soil, and the soil ecology reparation of the effect polluted with degrading pesticide, fertilizer residue simultaneously
Preparation.
In order to realize above-mentioned technical purpose, technical scheme proposed by the present invention is：
The restoration of the ecosystem preparation of degenerated soil, includes bacillus subtilis, Candida utilis after a kind of planting vegetable in greenhouse
Saccharomycete, Lactobacillus plantarum, Lactobacillus casei, trichoderma viride, Rhodopseudomonas palustris.
Preferably, the bacillus subtilis (Bacillus subtilis) is preserved in Chinese agriculture microorganism fungus kind guarantor
Administrative center is hidden, preserving number is ACCC10616；It is micro- that the candida utili (Candida utilis) is preserved in Chinese agriculture
Biological inoculum preservation administrative center, preserving number is ACCC20059；The bacillus subtilis (Bacillus subtilis) is protected
Chinese agriculture Microbiological Culture Collection administrative center is hidden in, preserving number is ACCC10616；The Lactobacillus plantarum
(Lactobacillus plantarum) is preserved in Chinese agriculture Microbiological Culture Collection administrative center, and preserving number is
ACCC11118；The Lactobacillus casei (Lactobacillus casei) is preserved in the management of Chinese agriculture Microbiological Culture Collection
Center, preserving number is ACCC10640；The trichoderma viride (Trichoderma viride) is preserved in Chinese agriculture microorganism
Culture presevation administrative center, preserving number is ACCC 30169；Rhodopseudomonas palustris (the Rhodopseudomonas
Palustris Chinese agriculture Microbiological Culture Collection administrative center) is preserved in, preserving number is ACCC10649.
On the other hand, present invention also offers after above-mentioned planting vegetable in greenhouse the restoration of the ecosystem preparation of degenerated soil preparation
Method, including it is following rapid：
Step one, produces the production of hybrid seeds
Bacillus subtilis (the Bacillus that will be bought from Chinese agriculture Microbiological Culture Collection administrative center
Subtilis, ACCC10616) nutrient broth fluid nutrient medium of transferring conical flask in, at 30-38 DEG C, 180-220rpm shakes
Culture 24-48 hours are swung, is taken out after culture medium muddiness；Candida utili (Candida utilis, ACCC20059) is transferred
In entering the conical flask of potato fluid nutrient medium, at 25-32 DEG C, 180-220rpm shaken cultivation 48-72 hours treat that culture medium is muddy
Take out after turbid；Lactobacillus plantarum (Lactobacillus plantarum, ACCC11118) is transferred into the cone of MRS fluid nutrient mediums
In shape bottle, 48-72 hours are cultivated at 30-42 DEG C, after the taking-up after culture medium muddiness；Lactobacillus casei (Lactobacillus
Casei, ACCC10640) MRS fluid nutrient mediums of transferring conical flask in, 30-42 DEG C cultivate 48-72 hours, wait to cultivate
Take out after base muddiness；Trichoderma viride (Trichoderma viride, ACCC 30169) is transferred into potato fluid nutrient medium
Conical flask in, at 25-32 DEG C, 180-220rpm shaken cultivation 48-72 hours are taken out after culture medium muddiness；The red vacation in marsh
Monad (Rhodopseudomonas palustris, ACCC10649) is transferred into the conical flask of nutrient broth fluid nutrient medium
In, at 30-38 DEG C, 180-220rpm shaken cultivation 24-48 hours are taken out after culture medium muddiness；It is then placed in 2-4 DEG C of refrigerator
Preserve stand-by；Viable bacteria number is more than 1.5 × 10 in the muddy generally bacterium solution of culture medium8cfu/ml。
Step 2, composite bacteria liquid is prepared and cultivated
(1) bacterial classification for cultivating step one is weighed by following percentage by weight：Bacillus subtilis 20-40%, product protein are false
Silk saccharomycete 10-20%, Lactobacillus plantarum 10-30%, Lactobacillus casei 10-20%, trichoderma viride 10-20%, marsh are red
Pseudomonad 5-10%, is then mixed to get premixing bacterium solution；；
(2) composite bacteria liquid is prepared：Premixing bacterium solution presses 1 by 1 by more than：80-120 ratios are put into the raw material of following proportioning
In：Molasses 5-10%, pure water 90-98%, are put into 28-32 DEG C of culture after fermentation tank stirs, close after pH is less than 4.00
Culture 12-15 days is closed, composite bacteria liquid is obtained final product；
3rd step, fermentation prepares restoration of the ecosystem preparation
(1) agricultural crop straw is collected and crushed：After crops harvesting, stalk field is shakeout, dries, is collected, and transports production back
Keep in plant area's booth, drying is kept in booth.Crushed stalk length 3-8 millimeter, stalk will keep drying.
(2) collection of animal wastes, process：Animal wastes are collected, standby in manure storage pond, the moisture content 85- of excrement is stored in
95%；
(3) by the composite bacteria liquid obtained by the agricultural crop straw after crushing, animal wastes, molasses and step 2 by weight
1：2-5：0.1-0.5：0.1-0.5, is well mixed in mixer.It is fitted in the aluminium foil bag with air bleeding valve and seals, is placed in 25-32
DEG C complete anaerobic fermentation of sweathouse two weeks, obtains final product the soil ecology renovation agent of solid forms.
Preferably, above-mentioned agricultural crop straw is rice straw, maize straw, wheat stalk etc.；
Preferably, above-mentioned animal wastes are cow dung, pig manure, chicken manure, sheep excrement etc..
The present invention is, by microbial technique, to start with from the rehabilitating soil ecosystem, increases the beneficial bacterium in soil, while
Using the united technical method of multi-cultur es, the growth of plant stock, strong sprout of loosening the soil is promoted to go back while the rehabilitating soil ecosystem
With the effect that degrading pesticide, fertilizer residue pollute.
Specific embodiment
The invention provides after a kind of planting vegetable in greenhouse degenerated soil restoration of the ecosystem preparation, include bacillus subtilis
Bacterium, candida utili bacterium, Lactobacillus plantarum, Lactobacillus casei, trichoderma viride, Rhodopseudomonas palustris.
The present invention is described in more detail below by specific embodiment, for a better understanding of the present invention,
But following embodiments are not intended to limit the scope of the invention.
Embodiment one
Step one, produces the production of hybrid seeds
The bacillus licheniformis bought from Chinese agriculture Microbiological Culture Collection administrative center is transferred into nutrient broth liquid
In the conical flask of body culture medium, at 38 DEG C, 220rpm shaken cultivations 24 hours are taken out after culture medium muddiness；Candida utilis ferment
Mother transfer potato fluid nutrient medium conical flask in, at 30 DEG C, 220rpm shaken cultivations 48 hours, after culture medium muddiness
Take out；Lactobacillus plantarum transfer MRS fluid nutrient mediums conical flask in, cultivate 48 hours at 37 DEG C, culture medium to be treated is muddy
After take out；Lactobacillus casei transfer MRS fluid nutrient mediums conical flask in, 37 DEG C cultivate 48 hours, culture medium to be treated is muddy
Take out after turbid；Trichoderma viride transfer potato fluid nutrient medium conical flask in, at 28 DEG C, 220rpm shaken cultivations 72 are little
When, take out after culture medium muddiness；Rhodopseudomonas palustris transfer nutrient broth fluid nutrient medium conical flask in, 35
DEG C, 220rpm shaken cultivations 48 hours are taken out after culture medium muddiness；It is then placed in 4 DEG C of Refrigerator stores stand-by；
Step 2, bacterial classification compound criteria
(1) bacterial classification for cultivating step one is weighed by following percentage by weight：Bacillus licheniformis 30%, Candida utilis ferment
Female bacterium 20%, Lactobacillus plantarum 20%, Lactobacillus casei 15%, trichoderma viride 10%, Rhodopseudomonas palustris 5%, mutually
It is mixed to get premixing bacterium solution；
(2) composite bacteria liquid is prepared：Premixing bacterium solution is pressed into 1：80 ratios are put in the raw material of following proportioning：It is molasses 5%, pure
Water purification 95%, is put into 28 DEG C of cultures after fermentation tank stirs, and closed culture 12 days, obtain final product compound bacteria after pH is less than 3.60
Liquid；
Step 3, fermentation prepares restoration of the ecosystem agent
(1) maize straw is collected and crushed
After corn straw harvesting, shakeout, dry, collect in field, transport back and kept in production plant area booth, keep in booth
It is dried.3 millimeters of crushed stalk length, stalk will keep drying.
(2) collection of cow dung, process
Fresh cow dung is collected, standby in manure storage pond, the moisture content 85-95% of excrement is stored in；
(3) prepared by the soil ecology renovation agent of solid forms
By the maize straw after crushing, cow dung, molasses and above-mentioned composite bacteria liquid by weight 1：4：0.15：0.15, stirring
It is well mixed in machine.It is fitted in the aluminium foil bag with air bleeding valve and seals, be placed in 28 DEG C of complete anaerobic fermentations of sweathouse two weeks, obtains final product
The restoration of the ecosystem preparation of degenerated soil after planting vegetable in greenhouse.
Embodiment two
In more than 10 DEG C of temperature, the soil ecology renovation agent 1-2 tons that embodiment one is obtained are applied in every mu of booth vegetable field,
Soil ecology renovation agent is ploughed under in soil；Watering keeps soil moisture 60-80%, then the soil life of uniform spraying liquid form
State renovation agent, consumption determines regarding soil regime；Uniformly spray the soil ecology renovation agent (consumption of a liquid form after 60 days again
It is identical with first time), after 3 months, soil remediation is basically completed.
Edaphon change table after the before processing of table 1
Therefore：The soil ecology renovation agent of the present invention can be such that the bacterium in soil and Population of Actinomycetes has significantly
Increase, the quantity of fungi has significant reduction, and the micro organism quantity in the amount of application and soil of soil ecology renovation agent is in positive
Close.
The specific embodiment of the present invention has been described in detail above, but it is intended only as example, and the present invention is not limited
It is formed on particular embodiments described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and
Replacement is also all among scope of the invention.Therefore, the impartial conversion made without departing from the spirit and scope of the invention and
Modification, all should cover within the scope of the invention.
Claims (6)
1. after a kind of planting vegetable in greenhouse degenerated soil restoration of the ecosystem preparation, it is characterised in that include bacillus subtilis,
Candida utili bacterium, Lactobacillus plantarum, Lactobacillus casei, trichoderma viride, Rhodopseudomonas palustris.
2. after a kind of planting vegetable in greenhouse according to claim 1 degenerated soil restoration of the ecosystem preparation, it is characterised in that
The bacillus subtilis (Bacillus subtilis) is preserved in Chinese agriculture Microbiological Culture Collection administrative center, preservation
Number be ACCC10616；The candida utili (Candida utilis) is preserved in Chinese agriculture Microbiological Culture Collection pipe
Reason center, preserving number is ACCC20059；It is micro- that the bacillus subtilis (Bacillus subtilis) is preserved in Chinese agriculture
Biological inoculum preservation administrative center, preserving number is ACCC10616；Lactobacillus plantarum (the Lactobacillus
Plantarum Chinese agriculture Microbiological Culture Collection administrative center) is preserved in, preserving number is ACCC11118；The cheese breast bar
Bacterium (Lactobacillus casei) is preserved in Chinese agriculture Microbiological Culture Collection administrative center, and preserving number is
ACCC10640；The trichoderma viride (Trichoderma viride) is preserved in the management of Chinese agriculture Microbiological Culture Collection
Center, preserving number is ACCC 30169；The Rhodopseudomonas palustris (Rhodopseudomonas palustris) is preserved in
Chinese agriculture Microbiological Culture Collection administrative center, preserving number is ACCC10649.
3. after a kind of a kind of planting vegetable in greenhouse as claimed in claim 1 the restoration of the ecosystem preparation of degenerated soil preparation side
Method, it is characterised in that comprise the following steps：
Step one, produces the production of hybrid seeds
Take bacillus subtilis, candida utili bacterium, Lactobacillus plantarum, Lactobacillus casei, the red vacation of trichoderma viride and marsh
Monad bacterial classification distinguishes amplification cultivation, and viable bacteria number is more than 1.5 × 10 in amplification cultivation to bacterium solution8cfu/ml；
Step 2, composite bacteria liquid is prepared
The bacterium solution of each bacterium obtained by step one is mixed according to the following ratio：
Obtain being pre-mixed bacterium solution；Then the premixing bacterium solution is pressed into 1：80-120 part by weight is added in culture medium and cultivated, and is obtained
To composite bacteria liquid；
Step 3, fermentation prepares restoration of the ecosystem preparation
By the composite bacteria liquid obtained by the agricultural crop straw after crushing, animal wastes, molasses and step 2 by weight 1：2-5：
0.1-0.5：The ratio of 0.1-0.5, is placed in mixer and is well mixed, and is subsequently placed in 25-32 DEG C of sealed environment complete anaerobism
Ferment two weeks, obtain final product the restoration of the ecosystem preparation of degenerated soil after planting vegetable in greenhouse.
4. preparation method according to claim 3, it is characterised in that be honey and water outside the culture medium in the step 2
It is formulated, it is 28-32 DEG C that premixing bacterium solution adds cultivation temperature in culture medium, and incubation time is 12-15 days.
5. preparation method according to claim 3, it is characterised in that agricultural crop straw is selected from paddy rice straw in the step 3
One or more in stalk, maize straw and wheat stalk.
6. preparation method according to claim 3, it is characterised in that animal wastes in the step 3 are selected from cow dung, pig
One or more in excrement, chicken manure and sheep excrement.
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Cited By (4)
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| CN107337515A (en) * | 2017-07-04 | 2017-11-10 | 北京百丰天下生物科技有限公司 | High organic fertilizer for soil remediation and preparation method thereof |
| CN111777469A (en) * | 2020-07-17 | 2020-10-16 | 盘锦鑫叶农业科技有限公司 | Greenhouse soil remediation agent based on reed leaves and spikes and soil remediation method thereof |
| CN113735651A (en) * | 2021-08-26 | 2021-12-03 | 黑龙江谷馨源肥业有限公司 | Soil remediation improver and preparation method thereof |
| IT202100015710A1 (en) * | 2021-06-16 | 2022-12-16 | Bioevo S R L | FERTILIZER FOR AGRICULTURAL LAND AND RELATED FERTILIZING METHOD |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107337515A (en) * | 2017-07-04 | 2017-11-10 | 北京百丰天下生物科技有限公司 | High organic fertilizer for soil remediation and preparation method thereof |
| CN111777469A (en) * | 2020-07-17 | 2020-10-16 | 盘锦鑫叶农业科技有限公司 | Greenhouse soil remediation agent based on reed leaves and spikes and soil remediation method thereof |
| IT202100015710A1 (en) * | 2021-06-16 | 2022-12-16 | Bioevo S R L | FERTILIZER FOR AGRICULTURAL LAND AND RELATED FERTILIZING METHOD |
| CN113735651A (en) * | 2021-08-26 | 2021-12-03 | 黑龙江谷馨源肥业有限公司 | Soil remediation improver and preparation method thereof |
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