CN101314550A - Soil improvement type composite biological organic liquid fertilizer - Google Patents
Soil improvement type composite biological organic liquid fertilizer Download PDFInfo
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- CN101314550A CN101314550A CNA200810039598XA CN200810039598A CN101314550A CN 101314550 A CN101314550 A CN 101314550A CN A200810039598X A CNA200810039598X A CN A200810039598XA CN 200810039598 A CN200810039598 A CN 200810039598A CN 101314550 A CN101314550 A CN 101314550A
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- Fertilizers (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the organic fertilizer technical field, in particular relating to a soil improvement type composite biological organic liquid fertilizer. The soil improvement type composite biological organic liquid fertilizer is prepared by combination of bifidobacterium, lactobacillus bulgaricus, lactobacillus casei, saccharomyces cerevisiae, bacillus subtilis, streptomyces hygroscopicus and so on. The compositions are all profitable strains sieved from soil, are cultured and multiplied by application of the biotechnology, added with organic substances and nutritive elements, and processed into the novel composite biological organic liquid fertilizer by means of chelating, and are favorable for recovering the ecosystem of soil organisms. Therefore, the product of the soil improvement type composite biological organic liquid fertilizer not only has the function of fertilization but also can improve the physical property and the biological property of the soil and have the function of improving the soil, and can be widely used for various crops.
Description
Technical field
The invention belongs to the organic fertilizer technical field, be specifically related to a kind of soil improvement type composite biological organic liquid fertilizer.
Background technology
In the past few decades, chemical fertilizer provides a large amount of chemical elements to synthesize fertile required raw material to farm crop as it, perform meritorious deeds never to be obliterated.But also caused the serious problems of soil acidification, sclerosis and disease and pest and toxin, the plant root receptivity is reduced significantly, the nutritive element of soil can not effectively utilize, the weak many sick major reasons of malnutritive crop especially.In addition, in the agricultural operation, use a large amount of chemical hormones and chemical pesticide, also brought pesticide residue and soil pollution problem.
Summary of the invention
The objective of the invention is to propose a kind of composite biological organic liquid fertilizer that helps soil improvement.
The soil improvement type composite biological organic liquid fertilizer that the present invention proposes is a kind of composition, and its composition weight proportioning is as follows:
A. bifidobacterium (Bifidobacterium bifidum) 10-20%
B. lactobacillus bulgaricus (Lactobacillus bulgavicus) 10-20%
C. lactobacterium casei (Lactobacillus casei) 10-20%
D. yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) 10-20%
E. subtilis (Bacillus subtilis) 10-30%
F. streptomyces hygroscopicus (Streptomyces hygroscopicus) 10-30%
Total amount satisfies 100%.
The blending process of above-mentioned composite biological organic liquid fertilizer is as follows:
1. make the bacterial classification source: in the laboratory, behind six bacterial screenings of above-mentioned A-F, purifying, drop into respectively in one liter of substratum after sterilizing and cultivate slaking, make volume production and use the bacterial classification source;
2. single volume production of planting: to six bacterial classification sources of above-mentioned A-F, make a large amount of aseptic culture mediums with a tonne retort respectively, amplify the upper limit with 50 times, gradually be amplified to desired volume, each amplification need be kept oxygenation, stirring and control its temperature between 25 ℃-30 ℃, and each incubation time is 5-10 days (different according to bacterial classification); Phosphoric acid potassium dihydrogen 0.6% in the used substratum of single kind volume production, comprehensive amino acid 2%, granulated sugar 1%, potential of hydrogen is 7; All be weight percentage.
3. mix the anti-cultivation of straw: pour into bacterial classification A～F in the substratum of mixing vessel simultaneously by consumption proportion, mixed culture adopts the natural medium behind the High Temperature Sterilization, main component is molasses (as the waste Molases of granulated sugar factory) 20%, egg 3%, analysis for soybean powder 1%, fresh milk 1%, trash fish 1%, potential of hydrogen serves as that buffering is adjusted into pH7 with 1% potassium primary phosphate; 25 ℃-30 ℃ of controlled temperature are kept stirring, oxygenation, after the affirmation post cure is finished, can pour storage tanks into and deposit and be packaged into commodity.
The used bacterial classification of this product source is for Guangdong Microbes Inst DSMZ.Cultivate through Ke Kai bio-engineering corporation microbe research center, identify the back employing with the bacterial classification comparison of screening in the soil.Bacterial classification comes the following row of source material:
Bifidobacterium (Bifidobacterium bifidum) GIM1.169 AS1.1852
Lactobacillus bulgaricus (Lactobacillus bulgavicus) GIM1.189 ACCC10638
Lactobacterium casei (Lactobacillus casei) GIM1.159 ATCC334
Yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) GIM2.40 AS2.399
Subtilis (Bacillus subtilis) GIM1.20 AS1.286
Streptomyces hygroscopicus (Streptomyces hygroscopicus) GIM4.50 GIM M4,
The present invention has following characteristics:
A. bacterial classification provides: the bacterial classification component all is the profitable strain that screens from soil, and Applied Biotechnology is cultivated breeding, the useful disease and pest and the recovery soil organisms ecosystem of contending with.
B. sequestering action: organic and nutritive element drops into cultivation simultaneously, and organic hydrolysis moment such as albumen and nutritive element chelating are processed into the NEW TYPE OF COMPOSITE bio-organic liquid fertilizer, and the nutriment in a fertilizer slowly-releasing is brought into play fertilizer efficiency and do not run off.
C. antagonism ferment: utilize the bacterial classification of slaking volume production to carry out hybrid cross and cultivate for the second time, cause violent antagonistic action, help the secretion that enzyme microb and antagonism become branchs. raising plant growth gesture and resistant to diseases and insects.
The invention has the beneficial effects as follows:
(1) can decompose organic matter: make the just fully fermentation of straw, people, fowl, animal manure.
(2) can improve soil physical property, biological.
(3) make fertile effect.
(4) degradable toxin suppresses disease and pest.
(5) can promote fertilizer efficiency.
The using method of this product:
1. normal soil: before the sowing, use three kilograms of dilutions of this product 100-300 doubly, on average intersperse among the 0.07 hectare face, the 15-25 centimetre buries.Or add water towards imposing in the 0.07 hectare, place and can sow two days later.
2. poor soil: before the sowing, use five kilograms of dilutions of this product 100-300 doubly, on average intersperse among the 0.07 hectare face, the 15-25 centimetre buries.Or add water towards imposing in the 0.07 hectare, place and can sow two days later.
3. seed soaks: after dilution 250-400 doubly soaks 25-40 minute, and the back sowing of drying in the shade on the tiling old newspaper.
4. germinateed and do not moved the seedbed of sanction: used 500-600 times of diluent.Every mu of 100-150 litre sprayed once in every 3-5 days, looked the seedbed water demand, watered for water.
5. sowed seedling: every mu is used 0.1 kilogram of this product to add water 50-60 kilogram dilution back pouring root, and after 8-10 days, 0.07 hectare doubly waters with one kilogram of dilution of this product 150-200, once a day, and triplicate.
6. sowed into strain: every mu is used one kilogram of dilution of this product 150-200 doubly to water root.Repeat every day, continuous three times.Membrane covering person is squeezed into by the space.
7. keep pouring: one kilogram of each month every mu of use later on, dilution 200-300 doubly waters root and branches and leaves.
This product major function is for promoting the ecological balance of soil improvement and crop periphery, and the usages of various crops be there is no particular requirement.
The crop that this product is suitable for: this product is a soil improvement agent, has to improve soil physics domestic animal, chemical and biological effect.Soil ecology is tended to be balanced, and can promote the procreation of profitable strain, make fertile short length, disease and pest contends with.This product is regardless of crop varieties, and soil health nature crop is healthy and strong.Be suitable for the various crops that model state comprises the long and short phase in vegetative period, food crop, fruit tree and forest or the like
Be some test-results below.
Improve soil physical property.Through in recent years to lake black earth, strong yellow soil, three kinds of soil fertility monitorings of Baijiang soil, the result shows, can improve soil porosity, make soil granularization, thick microaggregate improves, and increases structure (being) number, under the constant situation of total porosity, the ventilating slit increases by 0.3～0.6 times, and net porosity increases by 5.8%, and unit weight reduces by 10%; Strengthen the soil water-reataining fertilizer-keeping ability, improve field capacity 7.2%, the absorption nutrient, the validity of raising natural precipitation strengthens drought-resistant ability.
Improve the soil nutrient situation.But the circulation of soil material is quickened in the consumption of direct compensation soil fertility, promotes the growth of soil beneficial microorganism, improves the nutrient supply situation, culture fertility.By soil fertility monitoring in recent years, the result shows that the organic salt moisture soil of topsoil is brought up to 20.5g/kg from 19.4g/kg, and average year improves 0.28g/kg; And the Baijiang soil of amplification maximum rises to 11.6g/kg from 10.0g/kg, and average year increases 0.40g/kg, is showing and is improving the soil nutrient situation.
Improve crop yield.Through simultaneous test, the result shows, goes through 6 season of rice wheat crop, and paddy rice and wheat increase yield are showing, accumulative total wheat increase yield 150.4kg, average per season mu volume increase 50.1kg; Paddy rice accumulative total is increased production 170.6kg, average per season mu volume increase 56.8kg; The wheat rice is compared respectively according to volume increase 18.1% and 10.9%.Be some crop object lesson on probation below.
Embodiment
The invention is further illustrated by the following examples.
Embodiment 1
Organic liquid fertilizer proportioning by weight is as follows:
The A component: 10%,
The B component: 10%,
The C component: 10%,
The D component: 10%,
The E component: 30%,
The F component: 30%,
Production control requires:
The blending process of composite biological organic liquid fertilizer such as above-mentioned (consulting page 1). but when the third step mixed culture, after A～F inoculating proportion is pressed embodiment 1 listed ratio mixing, pour in the mixing vessel and an amount of substratum is provided, 25 ℃-30 ℃ of operation controlled temperature, keep stirring, oxygenation, make each bacterial classification compete to deposit antagonism. after confirming that the Secondary Fermentation slaking is finished, can pour storage tanks into and deposit and be packaged into commodity.
The composite biological organic liquid fertilizer of being produced according to embodiment 1, through field experiment, can increase the unity kernel structure of soil, the pathogeny bacterium is significantly warned few, and root rot is close to disappearance, and the ground nematode also disappears, and can improve the potential of hydrogen of soil, reduce in land for growing field crops vegetables plantation test disease, the harvest at root crop obviously promotes especially. and on toxicological test, guinea pig is used and goes up no abnormality seen.
Embodiment 1 field test:
Be responsible for carrying out the farmland covered by contract that the experimental plot piece is a peasant household, 2 mu of areas by agricultural technology extension center, suburb, Nanjing.Test period is 2004, and soil species is a river silt soil, and recording the soil organism before the test is 19.6g/kg, alkali-hydrolyzable nitrogen 109.8mg/kg, and rapid available phosphorus 8.3mg/kg, available potassium is 78.4mg/mg, soil fertility level is medium.For studying thing is cotton, and kind is anti-No. of state.Test design: divide two processing, do not establish repetition, 1.0 mu of every areas, other agronomic measures is consistent.Handle one for using liquid fertilizer of the present invention, processing two fertilising routinely compares.Test-results is as follows: the average plant height of sampling: handle one and be 110.4cm, handling two is 106.3cm, and the former exceeds 4.1cm than the latter; Investigate average fruit skill platform number: handling one is 15.6, is treated to 14.9, and the former mostly is 0.7 than the latter; Increase by 4.5%.The statistics individual plant is on average tied the number in age: handling one is 19.6, and handling two is 18.4, and the former Duos 1.2 than the latter, increases by 6.1%.Last per mu yield is young cotton: handling one is 218.7kg, and handling two is 204.9kg, and the former increases 13.8kg than the latter, increases 6.3%.The testing soil fertility state is significantly improved.The Soil structure situation also has clear improvement.
Embodiment 2
Organic liquid fertilizer proportioning by weight is as follows:
The A component: 10%,
The B component: 15%,
The C component: 15%,
The D component: 20%,
The E component: 20%,
The F component: 20%,
Production control requires:
The blending process of composite biological organic liquid fertilizer such as above-mentioned (consulting page 1). but when the third step mixed culture, after A～F inoculating proportion is pressed embodiment 2 listed ratios mixing, pour in the mixing vessel and an amount of substratum is provided, 25 ℃-30 ℃ of operation controlled temperature, keep stirring, oxygenation, make each bacterial classification compete to deposit antagonism. after confirming that the Secondary Fermentation slaking is finished, can pour storage tanks into and deposit and be packaged into commodity.
Composite biological organic liquid fertilizer according to embodiment 2 is produced through field experiment, can improve soil physical property, nematode also disappears on ground, organic matter decomposition is fast on the ground, and the fertilizer stink rapidly disappears, and newborn Bai Gen is healthy and strong, growth potential is strong. can improve the potential of hydrogen of soil simultaneously, improve the soil nutrient level, reduce harvest ahead of time in land for growing field crops vegetables plantation test disease, prolong the paracme. on toxicological test, guinea pig is used and goes up no abnormality seen.
Embodiment 2 field tests:
The trier is with example 1.The experimental plot piece is the farmland covered by contract of a peasant household, 2 mu of areas, and test period 2004, soil species is yellowish-white soil, recording the soil organism before the test is 17.4g/kg, alkali-hydrolyzable nitrogen 106.8mg/kg, rapid available phosphorus 92mg/kg, available potassium is 94.5mg/mg, soil fertility level is medium.Trial crops is a paddy rice, and kind is that force is educated No. 14 hybridisation rices of round-grained rice.Test design: divide two processing, do not establish repetition, 1 mu of every area, other agronomic measures is consistent.Handle one for using the liquid fertilizer of the present invention, handle two and use conventional fertilizer to compare.Test-results is as follows: the growing way proterties, and peak seedling, handling one is 29.4 ten thousand, and handling two is 28.9 ten thousand, and the former Duos 0.5 ten thousand than the latter, increases by 1.7%; The every strain root of stochastic sampling statistical average bar number, handling one is 87.2, and handling two is 85.4, and the former Duos 1.8 than the latter.The fringe kernel structure, one mu of number of productive ear: handling one is 17.5 ten thousand, and handling two is 17.0 ten thousand, and the former Duos 0.5 ten thousand than the latter, increases by 2.9%.Per mu yield: handling one is 579.7kg, and handling two is 521.6kg, and the former increases 58.1kg than the latter, increases by 10%.The testing soil fertility state was significantly improved than originally.The Soil structure situation also has clear improvement.
Embodiment 3
Organic liquid fertilizer set of dispense by weight is such as following:
A component: 20%
B component: 20%
C component: 20%
D component: 20%
E component: 10%
F component: 10%
Production control requires:
The blending process of composite biological organic liquid fertilizer such as above-mentioned (consulting page 1). but when the third step mixed culture, after A～F inoculating proportion is pressed embodiment 3 listed ratios mixing, pour in the mixing vessel and an amount of substratum is provided, 25 ℃-30 ℃ of operation controlled temperature, keep stirring, oxygenation, make each bacterial classification compete to deposit antagonism. after confirming that the Secondary Fermentation slaking is finished, can pour storage tanks into and deposit and be packaged into commodity.
The composite biological organic liquid fertilizer of being produced according to embodiment 3; through field experiment; can show and improve the soil nutrient situation; the decomposing force of fertilizer is obvious especially; disease resistance obviously promotes, and insect reproductivity goes down, club root and the Lock-out of nematode root nodule; porosity of soil improves greatly, and soil granularization, drainage, water-retentivity are obviously improved.Reduce in land for growing field crops vegetables plantation test disease, fertilizer efficiency promotes, and harvest is good. and on toxicological test, guinea pig is used and goes up no abnormality seen.
Embodiment 3 field tests:
The trier is with example 1.The experimental plot piece is the farmland covered by contract of a peasant household, 2 mu of areas, and test period 2004, soil species is yellowish-white soil, recording the soil organism before the test is 17.3g/kg, alkali-hydrolyzable nitrogen 96.9mg/kg, rapid available phosphorus 9.6mg/kg, available potassium is 98.1mg/mg, soil fertility level is medium.Trial crops is a watermelon, and kind is No. 2, no seed.Test design: piecemeal is handled, and does not establish repetition, 1 mu of every area, and other agronomic measures is consistent.Handle one for using the liquid fertilizer of the present invention, handle two and use conventional fertilizer to compare.Test-results is as follows: average single melon weight, and handling one be 4.46kg, and handling two be 3.90kg, and the former manys 0.56kg than the latter, and per mu yield: handling one is 2491.9kg, and processing two is 2152.8kg, and the former increases production 309.1kg than the latter, growth by 12.6%; And the kind of melon the former be significantly improved than the latter, for example the skin mean thickness is: the former is 0.9cm, the latter is 1.1cn.The center sugar degree: the former is 9.6, and the latter is 8.2.The testing soil fertility state was significantly improved than originally.The Soil structure situation also has clear improvement.
Claims (3)
1, a kind of soil improvement type composite biological organic liquid fertilizer is characterized in that set of dispense is such as following:
A. bifidobacterium 10-20%,
B. lactobacillus bulgaricus 10-20%,
C. lactobacterium casei 10-20%,
D. yeast saccharomyces cerevisiae 10-20%,
E. subtilis 10-30%,
F. streptomyces hygroscopicus 10-30%,
Total amount satisfies 100%.
2, a kind of preparation method of soil improvement type composite biological organic liquid fertilizer as claimed in claim 1 is characterized in that concrete steps are as follows:
(1). make the bacterial classification source: in the laboratory,, drop into respectively then in one liter of substratum after sterilizing and cultivate slaking, make volume production and use the bacterial classification source with described A-F item bacterial screening, purifying;
(2). single volume production of planting: to six bacterial classification sources of above-mentioned A-F, make a large amount of aseptic culture mediums with a tonne reaction narrow-necked earthen jar respectively,, be amplified to desired volume gradually with 50 times of amplification upper limits, each amplification need be kept oxygenation, stirring and control its temperature between 25 ℃-30 ℃, and each incubation time is 5-10 days; Phosphoric acid potassium dihydrogen 0.6% in the used substratum of single kind volume production, comprehensive amino acid 2%, granulated sugar 1%, potential of hydrogen is 7.
(3). mix the anti-cultivation of straw: bacterial classification A～F is poured into simultaneously by consumption proportion in the substratum of mixing vessel, and mixed culture adopts the natural medium behind the High Temperature Sterilization, and main component is molasses 20%, egg 3%, analysis for soybean powder 1%, fresh milk 1%, trash fish 1%, potential of hydrogen serves as that buffering is adjusted into pH7 with 1% potassium primary phosphate, 25 ℃-30 ℃ of controlled temperature are kept stirring, oxygenation, after the affirmation post cure is finished, can pour storage tanks into and deposit, and be packaged into commodity.
3, a kind of soil improvement type composite biological organic liquid fertilizer as claimed in claim 1 improve the soil and cultivation of crops in upright usefulness, it is held to levy and is that specific practice is as follows:
(1), normal soil: before the sowing, use described liquid fertile 3 kilograms of dilution 100-300 doubly, on average intersperse among the 0.07 hectare face, the 15-25 centimetre buries.Or add water towards imposing in the 0.07 hectare, place and can sow two days later;
(2), poor soil: before the sowing, use described liquid fertile 5 kilograms of dilution 100-300 doubly, on average intersperse among the 0.07 hectare face, the 15-25 centimetre buries; Or add water towards imposing in the 0.07 hectare, place and can sow two days later;
(3), seed soaks: after the fertile dilution of described liquid state 250-300 was doubly soaked 25-40 minute, the back of drying in the shade on the tiling old newspaper was sowed;
(4), germinateed and do not moved the seedbed of sanction: use the fertile 500-600 of described liquid state times diluent, every mu of 100-150 litre sprayed once in every 3-5 days, looked the seedbed water demand, watered for water;
(5), oneself sowing seedling: every mu is used 0.1 kilogram of this product to add water 50-60 kilogram dilution back pouring root, and after 8-10 days, 0.07 hectare dilutes 150-0 and doubly waters with described liquid fertile 1 kilogram, once a day, triplicate;
(6), oneself sows into strain: every mu is used described liquid fertile 1 kilogram of dilution 150-200 doubly to water root, and repeat every day, continuous three times; Membrane covering person is squeezed into by the space;
(7), keep pouring: one kilogram of each month every mu of use later on, dilution 200-300 doubly waters root and branches and leaves.
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| CN107446583A (en) * | 2017-07-31 | 2017-12-08 | 路域生态工程有限公司 | A kind of smolmitza soil conditioner host material and the plant raw-soil modification method of east steppe vegetation damaged zone |
| CN115215702A (en) * | 2022-07-17 | 2022-10-21 | 云南大微时代生物科技有限公司 | Composite biological organic liquid fertilizer for soil improvement and preparation and use methods thereof |
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