CN106478304A - Composite liquefied fertilizer and preparation method thereof - Google Patents
Composite liquefied fertilizer and preparation method thereof Download PDFInfo
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- CN106478304A CN106478304A CN201610989959.1A CN201610989959A CN106478304A CN 106478304 A CN106478304 A CN 106478304A CN 201610989959 A CN201610989959 A CN 201610989959A CN 106478304 A CN106478304 A CN 106478304A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
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- Pest Control & Pesticides (AREA)
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Abstract
The invention discloses a kind of composite liquefied fertilizer and preparation method thereof, by weight percentage, consisting of:Moriamin S 10~15%;Ribonucleic acid concentrate 3~4%;Potassium dihydrogen phosphate 2.0~5.0%;Chinese medicine fermented liquid 1.5~3.5%;Balance of water.The present invention is fermented to kaladana using bacillus subtilis, the combination state gibberellin gibberellin glucoside without physiologically active that can be in kaladana and gibberellin glucose ester go glycosylation process, it is converted into C19 class gibberellin, the physiologically active of kaladana is greatly improved, so as to substantially increase the effect of increasing production of crop.Due to Chinese medicine fermented liquid, in the case that GA content is extremely low, (50~500ng/ml) can also play fabulous effect of increasing production, will not both suppress plant growth, also do not result in gibberellin residual, greatly improve crop as foodsafety.
Description
Technical field
The present invention relates to a kind of liquid fertilizer, and in particular to a kind of composite liquefied fertilizer and preparation method thereof.
Background technology
At present, the solid-states such as nitrogenous, phosphorus, the chemical fertilizer of potassium and fraction animal wastes fertilizer is mainly applied in China farmland.In order to reach
To the purpose of volume increase, will repeatedly apply every year, in manpower and financial resources, very big burden be caused to peasant.Although animal wastes
The good but limited source of fertilizer efficiency, breath malodor, the large area for limiting animal wastes are used, and are applied chemical fertilizer in a large number and can be caused soil
Physique cracking even causes the underproduction, in addition, a large amount of productions of chemical fertilizer can cause the pollution of environment.Compared with solid-state fertilizer, liquid fertilizer
With fertilizer efficiency high, apply the advantages such as convenient, the low and environmental pollution of producing cost is little.China as Publication No. CN101913960
Patent of invention, discloses plant nutrition liquid fertilizer and preparation method thereof, and the composition that the liquid fertilizer is pressed includes:Moriamin S,
Ribonucleic acid concentrate, auxin concentrate, non-protein non-amino acid nitrogen, potassium dihydrogen phosphate, potassium oxide, zinc, boron,
Magnesium, Tong Heshui.Due to containing amino acid, auxin and ribonucleic acid in the product, plant rhizosphere soil can be progressively adjusted
Earth acid-base value, improves microbial activity, accelerates organic matter decomposition, improves soil aggregate, improves utilization rate of fertilizer, and crop is applied
With rear, to adverse circumstances such as low temperature, arid, salt damage, diseases resistivity enhancing.Wherein, auxin be by gibberellin bacterium
Kind through slant strains culture, after through shaking table shaken cultivation, then fermented and cultured in seeding tank, most after fermentation cylinder for fermentation
After culture, fermenting mixture is excluded, filter, filtrate is acidified with sulfuric acid, then PH is adjusted with sodium carbonate, concentrate, obtain final product.Due to red
The product of mycin strain fermentation is substantially C20 class gibberellin, and its activity is poor, and therefore application dosage is larger, easily produces work
Thing is remained, and causes food security hidden danger;In addition, auxin application dosage is larger, applies not and easily suppressed crop at that time
Growth even causes plant death, and the volume increase to crop on the contrary produces unfavorable effect.
Content of the invention
The technical problem to be solved in the present invention is to provide a kind of composite liquefied fertilizer, and the liquid fertilizer is good to the effect of increasing production of crop,
And nothing hormone residues.
The technical scheme that the present invention is provided is a kind of composite liquefied fertilizer, by weight percentage, consisting of:
Moriamin S 10~15%;
Ribonucleic acid concentrate 3~4%;
Chinese medicine fermented liquid 1.5~3.5%;
Potassium dihydrogen phosphate 2.0~5.0%;
Balance of water;
Wherein, Chinese medicine fermented liquid is obtained by following methods:Kaladana is crushed, is added water and stirs evenly, add kaladana's weight 5
~10% bacillus subtilis, ferment 7~10d, and centrifugation is filtered, and filtrate is concentrated into GA content for 50~500ng/ml,
As Chinese medicine fermented liquid;
Moriamin S is obtained by following methods:Take hair, the pH for adding 1~6 times of its weight be 1~3 hydrochloric acid molten
Liquid, 2~4h of acidolysis under the conditions of 50~80 DEG C, filter, filtrate is collected, amino acid content is concentrated into for 0.8~1.2g/100ml,
Obtain final product Moriamin S;
Ribonucleic acid concentrate is obtained by following methods:Distiller's yeast yeast is taken, with blackstrap as culture based on normal in fermentation tank
Temperature 10~15h of fermentation, adds the NaOH of its weight 0.8~1.2% in gained zymotic fluid, is heated to 70~80 DEG C of conditions
Lower 1~2h of alkaline hydrolysis, filters, and it is 10~15% weight that filtrate is concentrated into wherein rna content, obtains final product ribonucleic acid concentrate.
Kaladana (Morning Glory Seed) is convolvulaceous plant pharbitis nilChoisy Pharbitis nil (L.) Choisy
Or the dry mature seed of Pharbitis purpurea Pharbitis purpurea (L.) Voigt.Using bacillus subtilis to kaladana
Fermentation process is carried out, hay bacillus can secret out of protease, cellulase, hemicellulase, pectin during growth and breeding
Tens kinds of ectoenzymes such as enzyme, amylase, destroy the cell membrane of kaladana so that the smooth dissolution of active ingredient in kaladana;Secondly,
Fermentation of bacillus subtilis can be with the combination state gibberellin without physiologically active in kaladana -- gibberellin glucoside and
Gibberellin glucose ester is precursor, glycosylation through going, and will be converted into free state gibberellin in conjunction with state gibberellin, and greatly improve and lead
The physiologically active of Niu Zi.Due to the special selectively acting of bacillus subtilis, C19 class can will be converted into reference to state gibberellin red
There are 19 carbon atoms on mycin, i.e. each Gibberellin molecules, C19 class gibberellin physiologically active is higher than C20 class gibberellin, also one
Determine in degree, to improve the physiologically active of kaladana.Apply kaladana's fermentation liquid energy and dramatically increase chloroplaset in unit mesophyll cell
Quantity, and the form of chloroplaset also changed therewith, and chloroplaset cross-sectional area and length are all dramatically increased, meanwhile, class
Utricule film thickness increases, and interstitial volume diminishes, is conducive to utilization of the blade to luminous energy, improves the energy that luminous energy changes to chemical energy
Power.Moreover, the ratio that kaladana can improve crop chlorophyll a is applied, increases chlorophyll a/b value so that Chlorophyll absorption
While luminous energy ability is greatly improved, moreover it is possible to improve the ability that luminous energy changes into chemical energy, can prevent excess electron induction from living
Property oxygen accumulation, maintain plant body in active oxygen generation and remove between balance, it is to avoid there is crop cell and occur too early
Oxidative stress, Delaying Leaf-Senescence, so as to be greatly improved crop yield.
The addition of water is 2~10 times of kaladana's weight.The centrifugation be by the mixed liquor of fermentation process 5000~
5~10min is centrifuged under 8000r/min.
The weight ratio of blackstrap and distiller's yeast yeast is 100~150:1.
Present invention also offers the preparation method of above-mentioned composite liquefied fertilizer, comprises the following steps:
1) kaladana is crushed, adds water and stir evenly, add the bacillus subtilis of kaladana's weight 5~10%, fermentation 7~
10d, centrifugation, to filter, filtrate is concentrated into GA content for 50~500ng/ml, as Chinese medicine fermented liquid;
2) take hair, the pH for adding 1~6 times of its weight is 1~3 hydrochloric acid solution, under the conditions of 50~80 DEG C acidolysis 2~
4h, filters, and collects filtrate, is concentrated into amino acid content for 0.8~1.2g/100ml, obtains final product Moriamin S;
3) distiller's yeast yeast is taken, with blackstrap as culture based on 10~15h of normal temperature fermentation in fermentation tank, in gained zymotic fluid
The NaOH of its weight 0.8~1.2% is added, 1~2h of alkaline hydrolysis under the conditions of being heated to 70~80 DEG C, to filter, filtrate is concentrated into
Wherein rna content is 10~15% weight, obtains final product ribonucleic acid concentrate;
4) above-mentioned Chinese medicine fermented liquid, Moriamin S, ribonucleic acid concentrate are weighed by proportional quantity, adds proportional quantity
Potassium dihydrogen phosphate and water, mix, obtain final product.
Step 1) in, the addition of water is 2~10 times of kaladana's weight.
Step 1) in, the centrifugation is that the mixed liquor of fermentation process is centrifuged under 5000~8000r/min 5~10min.
Step 2) in, the weight ratio of blackstrap and distiller's yeast yeast is 100~150:1.
The composite liquefied fertilizer of the present invention, need to be by product by use after 100~400 times of dilutions when using.
Compared with prior art, beneficial effects of the present invention have:
1) contain amino acid, Chinese medicine fermented liquid and ribonucleic acid in the liquid fertilizer of the present invention, can progressively adjust plant rhizosphere soil
Earth acid-base value, improves microbial activity, accelerates organic matter decomposition, improves soil aggregate, improves utilization rate of fertilizer, and crop is applied
With rear, to adverse circumstances such as low temperature, arid, salt damage, diseases resistivity enhancing.
2) present invention is fermented to kaladana using bacillus subtilis, the knot without physiologically active that can be in kaladana
State gibberellin -- gibberellin glucoside and gibberellin glucose ester go glycosylation process for conjunction, are converted into C19 class gibberellin, significantly
The physiologically active of kaladana is improved, so as to substantially increase the effect of increasing production of crop.
3) as the physiologically active of the bacillus subtilis fermentation liquor of kaladana is extremely strong so as to extremely low in GA content
In the case of (50~500ng/ml) fabulous effect of increasing production can also be played, will not both suppress plant growth, also not result in red
Mycin is remained, and greatly improves crop as the security of food.
4) due to containing nutriments such as abundant amino acid, protein, carbohydrate, mineral elements in kaladana, fermented
After process, macromolecular substances are hydrolyzed to small-molecule substance, utilize beneficial to plant absorption.
Specific embodiment
The present invention is further elaborated for specific examples below, but not as a limitation of the invention.
Embodiment 1
Composite liquefied fertilizer formula be by weight percentage:
Moriamin S 10%;
Ribonucleic acid concentrate 3%;
Chinese medicine fermented liquid 1.5%;
Potassium dihydrogen phosphate 2.0%;
Balance of water.
Preparation method:
1) kaladana is crushed, adds 2 times of kaladana's weight of water to stir evenly, add the withered grass of kaladana's weight 5%
Bacillus, ferment 7d, then centrifugation 5min centrifugation under 5000r/min, filters, and filtrate is concentrated into GA content and is
50ng/ml, as Chinese medicine fermented liquid;
2) hair is taken, the pH for adding 1 times of its weight is 1 hydrochloric acid solution, acidolysis 2h under the conditions of 50 DEG C, filters, collects
Filtrate, is concentrated into amino acid content for 0.8g/100ml, obtains final product Moriamin S;
3) distiller's yeast yeast is taken, with blackstrap as culture based on normal temperature fermentation 10h in fermentation tank, blackstrap and distiller's yeast yeast
Weight ratio is 100:1, the NaOH of its weight 0.8% is added in gained zymotic fluid, alkaline hydrolysis 1h under the conditions of being heated to 70 DEG C,
Filter, it is 10% weight that filtrate is concentrated into wherein rna content, obtains final product ribonucleic acid concentrate;
4) above-mentioned Chinese medicine fermented liquid, Moriamin S, ribonucleic acid concentrate are weighed by proportional quantity, adds proportional quantity
Potassium dihydrogen phosphate and water, mix, obtain final product.
Comparative example 1
Moriamin S 5%;
Ribonucleic acid concentrate 3%;
Non-protein non-amino acid nitrogen 4%;
Auxin concentrate 1.5%;
Potassium dihydrogen phosphate 5.0%;
Potassium oxide 2.5%;
Zinc 0.25%;
Boron 0.6%;
Magnesium 0.25%;
Copper 0.25%;
Balance of water.
In comparative example 1, the preparation method of Moriamin S and ribonucleic acid concentrate is with embodiment 1, auxin
The preparation method of concentrate is as follows:
Gibberellin bacterial classification is taken first by slant strains culture 72h, after through shaking table shaken cultivation 17h, then in seeding tank
Fermented and cultured 17h, most after fermentation cylinder for fermentation culture 7d, excludes fermenting mixture, filters, and filtrate is acidified to pH2 with sulfuric acid,
PH4.5 is adjusted again with sodium carbonate, the filtrate after Secondary Control pH is concentrated into GA content for 200 μ g/ml, obtains final product.
By the liquid fertilizer of embodiment 1, comparative example 1,200 times of dilute with water is used for spraying for paddy rice respectively, and observation product is to paddy rice
The impact of yield.
Conventional fertilizer application (CK, Fertilizer in Rice fertilizer amount N, P is being carried out to paddy rice2O5、K2O is respectively 20kg/ mu, 5kg/ mu, 7kg/ mu)
On the basis of, if blank group, experimental group and control group, blank group spray clear water, experimental group and control group spray embodiment 1 respectively
Liquid fertilizer and comparative example 1 liquid fertilizer, experiment area be 300m2.Each group is respectively after rice seedling is turned green, before heading, paddy rice life
After in length, which respectively sprays 1 time, and the amount of spraying is identical, sprays 4 times altogether.
As a result show, experimental group compared with blank group, per mu volume increase 45~55%, compared with control group, per mu volume increase 7~
20%.
Embodiment 2
Composite liquefied fertilizer formula be by weight percentage:
Moriamin S 15%;
Ribonucleic acid concentrate 4%;
Chinese medicine fermented liquid 3.5%;
Potassium dihydrogen phosphate 5.0%;
Balance of water.
Preparation method:
1) kaladana is crushed, adds 10 times of kaladana's weight of water to stir evenly, add the withered of kaladana's weight 10%
Careless bacillus, ferment 10d, then centrifugation 10min centrifugation under 8000r/min, filters, and filtrate is concentrated into GA content and is
500ng/ml, as Chinese medicine fermented liquid;
2) hair is taken, the pH for adding 6 times of its weight is 3 hydrochloric acid solution, acidolysis 4h under the conditions of 80 DEG C, filters, collects
Filtrate, is concentrated into amino acid content for 1.2g/100ml, obtains final product Moriamin S;
3) distiller's yeast yeast is taken, with blackstrap as culture based on normal temperature fermentation 15h in fermentation tank, blackstrap and distiller's yeast yeast
Weight ratio is 150:1, the NaOH of its weight 1.2% is added in gained zymotic fluid, alkaline hydrolysis 2h under the conditions of being heated to 80 DEG C,
Filter, it is 15% weight that filtrate is concentrated into wherein rna content, obtains final product ribonucleic acid concentrate;
4) above-mentioned Chinese medicine fermented liquid, Moriamin S, ribonucleic acid concentrate are weighed by proportional quantity, adds proportional quantity
Potassium dihydrogen phosphate and water, mix, obtain final product.
By the liquid fertilizer of embodiment 2, comparative example 1,100 times of dilute with water is used for spraying for oranges and tangerines respectively, and observation product is to oranges and tangerines
The impact of yield.
Oranges and tangerines are being carried out conventional fertilizer application (CK, the abundant moral in Loudi-oranges and tangerines special fertilizer, spring by 60 plants of fruit tree for selecting growing way consistent
Fertilizer is each with strong fertilizer to apply 2kg/ strain) on the basis of, if blank group, experimental group and control group, per organizing 20 plants, blank group is sprayed clearly
Water, experimental group and control group spray the liquid fertilizer of the liquid fertilizer of embodiment 2 and comparative example 1 respectively.Each group respectively in the oranges and tangerines budding period and
Fruit expanding period is sprayed, and each period sprays 2 times (spraying interval every time 10 days).
As a result show, compared with blank group, per mu is increased production 50~55% to experimental group, compared with control group, per mu of volume increase 11
~19%.In the experimental plot of experimental group, red spider, longicorn, leaf miner significantly reduce.
Embodiment 3
Composite liquefied fertilizer formula be by weight percentage:
Moriamin S 12.5%;
Ribonucleic acid concentrate 3.5%;
Chinese medicine fermented liquid 2.5%;
Potassium dihydrogen phosphate 4.0%;
Balance of water.
Preparation method:
1) kaladana is crushed, adds 8 times of kaladana's weight of water to stir evenly, add the withered grass of kaladana's weight 8%
Bacillus, ferment 9d, then centrifugation 8min centrifugation under 6000r/min, filters, and filtrate is concentrated into GA content and is
200ng/ml, as Chinese medicine fermented liquid;
2) hair is taken, the pH for adding 3 times of its weight is 2 hydrochloric acid solution, acidolysis 3h under the conditions of 60 DEG C, filters, collects
Filtrate, is concentrated into amino acid content for 1.0g/100ml, obtains final product Moriamin S;
3) distiller's yeast yeast is taken, with blackstrap as culture based on normal temperature fermentation 12h in fermentation tank, blackstrap and distiller's yeast yeast
Weight ratio is 125:1, the NaOH of its weight 0.8~1.2%, alkali under the conditions of being heated to 75 DEG C is added in gained zymotic fluid
Solution 1.5h, filters, and it is 12% weight that filtrate is concentrated into wherein rna content, obtains final product ribonucleic acid concentrate;
4) above-mentioned Chinese medicine fermented liquid, Moriamin S, ribonucleic acid concentrate are weighed by proportional quantity, adds proportional quantity
Potassium dihydrogen phosphate and water, mix, obtain final product.
By the liquid fertilizer of embodiment 3, comparative example 1,400 times of dilute with water is used for spraying for tomato respectively, and observation product is to tomato
The impact of yield.
Conventional fertilizer application (CK, tomato dose N, P is being carried out to tomato2O5、K2O is respectively 13kg/ mu, 8kg/ mu, 21kg/
Mu) on the basis of, if blank group, experimental group and control group, per group 20 plants, blank group spray clear water, experimental group and control component
The liquid fertilizer of embodiment 3 and the liquid fertilizer of comparative example 1 are not sprayed.Experiment area is 300m2, each group respectively tomato seedling and florescence,
Spray after first time fruit picking, spray after second fruit picking, spray 4 times altogether.
As a result show, compared with blank group, per mu is increased production 45~50% to experimental group, compared with control group, per mu of volume increase
11.5~25%.In the experimental plot of experimental group, aphid, trialeurodes vaporariorum are significantly reduced.
Embodiment 4
Composite liquefied fertilizer formula be by weight percentage:
Moriamin S 10%;
Ribonucleic acid concentrate 4%;
Chinese medicine fermented liquid 3.5%;
Potassium dihydrogen phosphate 2.0%;
Balance of water.
Preparation method:
1) kaladana is crushed, adds 2 times of kaladana's weight of water to stir evenly, add the withered grass of kaladana's weight 10%
Bacillus, ferment 7d, then 5~10min of centrifugation centrifugation under 8000r/min, filters, and filtrate is concentrated into GA content and is
50ng/ml, as Chinese medicine fermented liquid;
2) hair is taken, the pH for adding 6 times of its weight is 1 hydrochloric acid solution, acidolysis 2h under the conditions of 80 DEG C, filters, collects
Filtrate, is concentrated into amino acid content for 1.2g/100ml, obtains final product Moriamin S;
3) distiller's yeast yeast is taken, with blackstrap as culture based on normal temperature fermentation 10h in fermentation tank, blackstrap and distiller's yeast yeast
Weight ratio is 150:1, the NaOH of its weight 0.8% is added in gained zymotic fluid, alkaline hydrolysis 1h under the conditions of being heated to 80 DEG C,
Filter, it is 15% weight that filtrate is concentrated into wherein rna content, obtains final product ribonucleic acid concentrate;
4) above-mentioned Chinese medicine fermented liquid, Moriamin S, ribonucleic acid concentrate are weighed by proportional quantity, adds proportional quantity
Potassium dihydrogen phosphate and water, mix, obtain final product.
By the liquid fertilizer of embodiment 4, comparative example 1,300 times of dilute with water is used for spraying for capsicum respectively, and observation product is to capsicum
The impact of yield.
Conventional fertilizer application (CK, capsicum dose N, P is being carried out to capsicum2O5、K2O respectively for 18kg/ mu, 8.3kg/ mu,
21.3kg/ mu) on the basis of, if blank group, experimental group and control group, per 20 plants of group, blank group spray clear water, experimental group and right
The liquid fertilizer of embodiment 4 and the liquid fertilizer of comparative example 1 are sprayed respectively according to group.Experiment area is 300m2, each group respectively in chilli seedling and
Spray after florescence, first time fruit picking, spray after second fruit picking, spray 4 times altogether.
As a result show, compared with blank group, per mu is increased production 50~55% to experimental group, compared with control group, per mu of volume increase 11
~19%.In the experimental plot of experimental group, aphid, red spider, mite class are significantly reduced.
Claims (8)
1. composite liquefied fertilizer, it is characterised in that:By weight percentage, consisting of:
Moriamin S 10~15%;
Ribonucleic acid concentrate 3~4%;
Chinese medicine fermented liquid 1.5~3.5%;
Potassium dihydrogen phosphate 2.0~5.0%;
Balance of water;
Wherein, Chinese medicine fermented liquid is obtained by following methods:Kaladana is crushed, is added water and stirs evenly, add kaladana's weight 5~
10% bacillus subtilis, ferment 7~10d, and centrifugation is filtered, and filtrate is concentrated into GA content for 50~500ng/ml, i.e.,
For Chinese medicine fermented liquid;
Moriamin S is obtained by following methods:Hair is taken, the pH for adding 1~6 times of its weight is 1~3 hydrochloric acid solution, in
2~4h of acidolysis under the conditions of 50~80 DEG C, filters, and collects filtrate, is concentrated into amino acid content for 0.8~1.2g/100ml, obtains final product
Moriamin S;
Ribonucleic acid concentrate is obtained by following methods:Distiller's yeast yeast is taken, is sent out based on normal temperature in fermentation tank as culture with blackstrap
10~15h of ferment, adds the NaOH of its weight 0.8~1.2%, alkali under the conditions of being heated to 70~80 DEG C in gained zymotic fluid
1~2h of solution, filters, and it is 10~15% weight that filtrate is concentrated into wherein rna content, obtains final product ribonucleic acid concentrate.
2. composite liquefied fertilizer according to claim 1, it is characterised in that:The addition of water is the 2~10 of kaladana's weight
Times.
3. composite liquefied fertilizer according to claim 1, it is characterised in that:Described centrifugation be by the mixed liquor of fermentation process
5~10min is centrifuged under 5000~8000r/min.
4. composite liquefied fertilizer according to claim 1, it is characterised in that:The weight ratio of blackstrap and distiller's yeast yeast is 100
~150:1.
5. any one of claims 1 to 3 composite liquefied fertilizer preparation method, it is characterised in that:Comprise the following steps:
1) kaladana is crushed, adds water and stir evenly, the bacillus subtilis of kaladana's weight 5~10% is added, ferment 7~10d,
Centrifugation, filters, and filtrate is concentrated into GA content for 50~500ng/ml, as Chinese medicine fermented liquid;
2) hair is taken, the hydrochloric acid of 1~6 times of its weight is added, 2~4h of acidolysis under the conditions of 50~80 DEG C, filter, filtrate is collected,
Amino acid content is concentrated into for 0.8~1.2g/100ml, obtains final product Moriamin S;
3) distiller's yeast yeast is taken, with blackstrap as culture based on 10~15h of normal temperature fermentation in fermentation tank, is added in gained zymotic fluid
The NaOH of its weight 0.8~1.2%, 1~2h of alkaline hydrolysis under the conditions of being heated to 70~80 DEG C, to filter, filtrate is concentrated into wherein
Rna content is 10~15% weight, obtains final product ribonucleic acid concentrate;
4) above-mentioned Chinese medicine fermented liquid, Moriamin S, ribonucleic acid concentrate are weighed by proportional quantity, adds the phosphorus of proportional quantity
Acid dihydride potassium and water, mix, and obtain final product.
6. according to claim 5 composite liquefied fertilizer preparation method, it is characterised in that:Step 1) in, the addition of water
2~10 times for kaladana's weight.
7. according to claim 5 composite liquefied fertilizer preparation method, it is characterised in that:Step 1) in, the centrifugation is
The mixed liquor of fermentation process is centrifuged under 5000~8000r/min 5~10min.
8. according to claim 5 composite liquefied fertilizer preparation method, it is characterised in that:Step 2) in, blackstrap and wine
The weight ratio of bent yeast is 100~150:1.
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CN101913960A (en) * | 2010-07-24 | 2010-12-15 | 周冠旻 | Plant nutrition liquid fertilizer and preparation method thereof |
CN102276326A (en) * | 2011-07-04 | 2011-12-14 | 中国农业科学院农业资源与农业区划研究所 | Sargassum organic liquid fertilizer and preparation method thereof |
CN103011921A (en) * | 2012-12-05 | 2013-04-03 | 河海大学 | Preparation method of biological organic and inorganic mixed liquid fertilizer |
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