The implantation methods of capsicum
The application is application number 201010514217.6, the applying date: on October 21st, 2010, denomination of invention is the divisional application of " nutrient of a kind of Promoting plant growth and preparation method thereof ".
Technical field
The present invention relates to the implantation methods of the implantation methods of a kind of capsicum, particularly a kind of capsicum.
Background technology
Greatly develop plant husbandry, forestry, promote that crop yield increases income, make our country green, it is the problem that various countries scientist pays close attention to research, the growth of plant be unable to do without and supplements the nutrients, and present plant husbandry, mainly by executing N,P,K fertilizers and organic manure promotes crop growth, use too much fertilizer very large on the quality and flavor impact of crops, be unfavorable for development and food security and the environmental protection of green agriculture.Therefore, people have studied growth hormone relevant to nucleic acid, nutrient, fertilizer.In theory: nucleic acid is the basic substance that forms life, is the basis of metabolism and energy metabolism, and the character of nucleic acid and the difference of quantity can cause the diversity of biological species and growth conditions.For congener life entity, the quantity of nucleic acid and the difference of existence form have determined prosperous the declining of power of this life entity.Therefore, nucleic acid substances has irreplaceable effect for the output raising in crops and even the production of whole vegetative kingdom and product quality improvement; Particularly several nucleotides salt and deoxyribonucleoside hydrochlorate and the cooperations thereof of the little molecule of nucleic acid such as nucleosides, nucleotide, pyrimidine and purine, the not only growth of regulating plant very effectively, can be used as again nutrient source is absorbed rapidly by plant, strengthen plant root vigor, improve plant anti-adversity ability, thus improve crop output, improve product quality.The little molecule of nucleic acid has enhancing improving activity of root system to plant, improves the absorption of root to phosphorus, potash fertilizer; Improve the activity of crops malate dehydrogenase and succinic acid enzyme, promote the assimilational capacity of plant, strengthen photosynthetic intensity, to plant growth have regulate and trophism (Wang Xi etc. the research [J] of the application and function mechanism of nucleic acid degradation product in Rice Production. Acta Biochimica et Biophysica Sinica, 1975,7 (1): 31-39).Chinese patent literature (publication number: 101186534) disclose a kind of preparation method who contains nucleic acid degradation product foliage fertilizer, concrete step is: 1., lixiviate nuclease, 2., dissolve ribonucleic acid (alkali lye hydrotropy), 3., the hydrolysis of nucleic acid, must contain
There are the little molecules of multiple nucleic acids such as nucleosides, nucleotide, purine, pyrimidine and a small amount of reducing sugar and few big or middle, trace element, the method is taking ribonucleic acid as raw material, with enzymatic hydrolysis, exist production cost high, degrade not thorough, seldom, containing deoxynucleotide composition, in mixed system, nucleotide salt yield is low, the mutual mixing ratio imbalance of various nucleosides hydrochlorates and deoxynucleotide salt face is very large on result of use impact.Chinese patent literature (publication number: 101050143) disclose a kind of nutrient that promotes crop growth, it is made up of Ribavirin and glucose, production and the guanosine of Ribavirin are in close relations, and this patent exists Ribavirin high cost, the unconspicuous problem of practical effect; Chinese patent literature (publication number: the preparation method who 1238323) discloses a kind of nucleic acid foliage fertilizer, the degraded of its nucleic acid adopts the method for alkaline hydrolysis, degradation product is purine and pyrimidine, there is equally nucleic acid high cost in this patent, various nucleosides hydrochlorates and the mutual mixing ratio of deoxynucleotide salt face are unreasonable, affect the problem of result of use.
Summary of the invention
The object of this invention is to provide the implantation methods of a kind of capsicum, its production cost is low, and method is simple, is easy to large-scale production and popularization; In mixed system, nucleotide salt yield is high, and various nucleosides hydrochlorates and the mutual mixing ratio of deoxynucleotide salt face are reasonable, nontoxic, harmless, noresidue, without any adverse side effect; Increase production, increase income, synergy, strengthen resistance, improve quality effective;
The present invention is achieved in that
An implantation methods for capsicum, during chili growth, uses nutrient; Described nutrient is made up of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, amino acid, and its percentage by weight is: nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 10-30%, amino acid 70-90%; Nucleosides hydrochlorate in described nutrient and deoxyribonucleoside hydrochlorate are taking nucleic acid as raw material:
1. the ratio mixed nucleus bronsted lowry acids and bases bronsted lowry liquid that the hydrolysis of nucleic acid is 1:20-30 according to nucleic acid, alkali lye weight ratio, alkali lye is
5.5-7N, under 30-45 DEG C of condition, stirs 10-14 hours, obtains nucleosides hydrochlorate and deoxyribonucleoside acid salt solution, filters, and filtrate is adjusted pH8-8.5, reduced pressure concentration;
2. decolour, dry concentrate added to activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 10-25%;
3. crystallization is dissolved nucleosides hydrochlorate and the deoxyribonucleoside hydrochlorate after doing with clear water, and its solution adds alcohol separates out, and crystallization is taken out
Be filtered dry dry, the white nucleosides hydrochlorate and the deoxyribonucleoside hydrochlorate that obtain;
Described nucleosides hydrochlorate mainly refers to: 5 '-adenylate dipotassium, 5 '-bird adenylate dipotassium, 5 '-cytidylic acid dipotassium, 5 '-uridylic acid dipotassium, the sylvite of four kind 5 '-mixed nucleotides; Deoxyribonucleoside hydrochlorate refers to: 5 '-deoxyadenylic acid dipotassium, 5 '-deoxidation bird adenylate dipotassium, 5 '-deoxycytidylic acid dipotassium, 5 '-deoxyuridylic acid dipotassium, 5 '-deoxythymidylic acid dipotassium, the di-potassium of five kind 5 '-deoxidation mixed nucleotides;
Time and concentration that described growing period is used nutrient are: at initial bloom stage and full-bloom stage respectively once, and concentration 200-300ppm, mu volume increase 40%~80%.
?the implantation methods of capsicum: nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are nucleotide sylvite and deoxyribonucleoside acid potassium salt.
The implantation methods of capsicum: nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are ribonucleotide sodium salt and deoxynucleotide sodium salt.
The implantation methods of capsicum: the sugar in described nutrient is glucose or sucrose or fructose.
The implantation methods of capsicum: the sugar in described nutrient is glucose or sucrose or fructose.
The implantation methods of capsicum: in the nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate of described nutrient, percentage by weight is: nucleosides hydrochlorate 85-99%, deoxyribonucleoside hydrochlorate 1-15%.
The implantation methods of capsicum: the nucleosides hydrochlorate in described nutrient and deoxyribonucleoside hydrochlorate are taking nucleic acid as raw material:
1., the hydrolysis of the nucleic acid ratio mixed nucleus bronsted lowry acids and bases bronsted lowry liquid that is 1:20-30 according to nucleic acid, buck weight ratio, alkali lye is 5.5-7N, under 30-45 DEG C of condition, stir 10-14 hours, obtain nucleosides hydrochlorate and deoxyribonucleoside acid salt solution, filter, filtrate is adjusted pH8-8.5, reduced pressure concentration;
2., decolouring, dry concentrate is added to activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 10-25%;
3., crystallization dissolves nucleosides hydrochlorate and the deoxyribonucleoside hydrochlorate after dry with clear water, its solution adds alcohol separates out, and crystallization suction filtration is dry, the white nucleosides hydrochlorate and the deoxyribonucleoside hydrochlorate that obtain.
The nutrient of Promoting plant growth, it is mainly made up of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, auxiliary agent, and its percentage by weight is: nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 10-80%, auxiliary agent 20-90%.
The nutrient of Promoting plant growth, in nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, percentage by weight is: nucleosides hydrochlorate 85-99%, deoxyribonucleoside hydrochlorate 1-15%.
The nutrient of Promoting plant growth, in nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, percentage by weight is: nucleosides hydrochlorate 88-96%, deoxyribonucleoside hydrochlorate 4-12%.
The nutrient of Promoting plant growth, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and auxiliary agent, percentage by weight is:
Nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 10-30%, auxiliary agent 70-90%.
The nutrient of Promoting plant growth, auxiliary agent is sugar or amino acid.
The nutrient of Promoting plant growth, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are sodium salt or sylvite.
The preparation method of the nutrient of Promoting plant growth provided by the present invention, concrete steps are as follows: taking nucleic acid as raw material, be mainly that to adopt the nucleic acid of dry method or wet production be raw material for mycelium from fermentation plant fermentation:
1., the hydrolysis of the nucleic acid ratio mixed nucleus bronsted lowry acids and bases bronsted lowry liquid that is 1:20-30 according to nucleic acid, buck weight ratio, alkali lye is 5.5-7N, under 30-45 DEG C of condition, stir 10-14 hours, obtain nucleosides hydrochlorate and deoxyribonucleoside acid salt solution, filter, filtrate is adjusted pH8-8.5, reduced pressure concentration;
2., decolouring, dry concentrate is added to activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 10-25%;
3., crystallization dissolves nucleosides hydrochlorate and the deoxyribonucleoside hydrochlorate after dry with clear water, its solution adds alcohol separates out, and crystallization suction filtration is dry, obtains white nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and water soluble mixt.
4., nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and water soluble mixt are pulverized, cross 200 mesh sieves and mix with auxiliary agent again.
In nucleic acid hydrolysis process described in this preparation method, alkali lye used is sodium hydroxide or potassium hydroxide solution.
In the present invention, nucleosides hydrochlorate mainly refers to: 5 '-adenylate disodium or potassium, 5 '-bird adenylate disodium or potassium, 5 '-cytidylic acid disodium or potassium, sodium salt or the sylvite of four kind 5 '-mixed nucleotides of 5 '-uridine monophosphate disodium or potassium; Deoxyribonucleoside hydrochlorate refers to: 5 '-desoxyadenossine acid disodium or potassium, 5 '-deoxidation bird adenylate disodium or potassium, 5 '-deoxycytidine acid disodium or potassium, 5 '-BrdU acid disodium,, sodium salt or the sylvite of five kind 5 '-deoxidation mixed nucleotides of 5 '-deoxyribosylthymine acid disodium or potassium.The source of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate is for taking nucleic acid as raw material, be mainly mycelium from fermentation plant fermentation to adopt the nucleic acid of dry method or wet production be raw material, this kind of nucleic acid is taking ribonucleic acid (RNA) as a small amount of deoxidation core of main mixing
Ribosomal ribonucleic acid (DNA), is hydrolyzed and makes with alkali lye; Auxiliary agent is mainly derived from two aspects: 1, the nucleic acid of dry method or wet production also contains the impurity such as sugar, protein, various metals or nonmetallic ion, has amino acid, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc. after hydrolysis; 2, exogenous adding: sugar (reducing sugar, non reducing sugar: as glucose, sucrose also have fructose etc.), amino acid, big or middle, micro-etc.
The present invention adopt mainly by nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, the Promoting plant growth nutrient of auxiliary agent composition and for taking nucleic acid as raw material, mainly from fermentation plant (Gourmet Powder Factory, brewery, antibiotics plant, yeast factory, Amino Acid Factory, paper mill, activated sludge after waste water treatment, petro-jelly fermentation etc.) to adopt the nucleic acid of dry method or wet production be the preparation method of raw material hydrolysis for by product (offcuts) mycelium after fermentation, (add the product of Ribavirin with existing product and method, the product being directly hydrolyzed with ribonucleic acid, enzyme solution) compare and have the following advantages: 1, it is low that mycelium from fermentation plant fermentation is produced its production cost of ribonucleic acid, be easy to large-scale production and popularization, 2, the nucleic acid that mycelium is produced is containing useful impurity, in addition basic hydrolysis method, technique is simple, in mixed system, nucleotide salt yield is high, and various nucleosides hydrochlorates and the mutual mixing ratio of deoxynucleotide salt face are reasonable, and amino acid, sugar part and other organic-inorganic auxiliary agent content are higher, therefore, increasing both production and income synergy, strengthens resistance, improves quality effective, 3, nontoxic, harmless, noresidue, without any adverse side effect, 4, be widely used in grain, cotton, oil, cigarette, tea, really, plant and the bacterium mushroom series products such as dish, traditional Chinese medicine, flowers, forest.
Embodiment
Embodiment 1: get 1 gram of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, 6 grams of glucose, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient.
Embodiment 2: get 1 gram of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, 2 grams of sucrose, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient.
Embodiment 3: get 2 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, 8 grams of glucose, nucleosides hydrochlorate and de-
Oxygen nucleosides hydrochlorate is pulverized, and mixes with glucose, obtains nutrient.
Embodiment 4: get 1.5 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, 8.5 grams of glucose, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient.
Embodiment 5: get 1 gram of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, 9 grams of glucose, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient.
Embodiment 6: get 2.5 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, 7.5 grams of glucose, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient.
Embodiment 7: get 4 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, 6 grams, fructose, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient.
Embodiment 8: get 5 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, 5 grams of glucose, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient.
Embodiment 9: get 1 gram, nucleotide potassium and deoxynucleotide potassium, 9 grams of glucose, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, obtain nutrient.
Embodiment 10: get 1.5 grams, nucleotide potassium and deoxynucleotide potassium, 8.5 grams of glucose, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, obtain nutrient.
Embodiment 11: get 2 grams, nucleotide potassium and deoxynucleotide potassium, 8 grams of glucose, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, obtain nutrient.
Embodiment 12: get 2.5 grams, nucleotide potassium and deoxynucleotide potassium, 7.5 grams of glucose, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, obtain nutrient.
Embodiment 13: get 3 grams, nucleotide potassium and deoxynucleotide potassium, 7 grams of glucose, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, obtain nutrient.
Embodiment 14: get 1.5 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, 4.5 grams of glucose, 4 grams, amino acid, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix with glucose, obtain nutrient.
Embodiment 15: get 2 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, 4 grams of glucose, 4 grams, amino acid, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix with glucose, obtain nutrient.
Embodiment 16: get 3 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, 4 grams of glucose, 3 grams, amino acid, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix with glucose, obtain nutrient.
Embodiment 17: get 2 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, 8 grams, amino acid, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix, and obtain nutrient.
Embodiment 18: by 100 grams of the crude product nucleic acid making from the mycelium of fermentation plant's by product by dry method or wet method, be dissolved in the sodium hydroxide solution of 3 liters of 6.3N, under 37 DEG C of conditions, stir 12 hours, winner will refer to and contain: 5 '-adenylate disodium, 5 '-bird adenylate disodium, 5 '-cytidylic acid disodium, sodium salt (the deoxyribonucleoside hydrochlorate in addition: 5 '-desoxyadenossine acid disodium of four kind 5 '-mixed nucleotides of 5 '-uridine monophosphate disodium, 5 '-deoxidation bird adenylate disodium, 5 '-deoxycytidine acid disodium, 5 '-BrdU acid disodium, , sodium salt and the amino acid of five kind 5 '-deoxidation mixed nucleotides of 5 '-deoxyribosylthymine acid disodium, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc.) solution, filter, filtrate is neutralized to pH8-8.5, be evaporated to appropriate, concentrate is added to activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 15%, with nearly dry new-nucleo and the deoxyribonucleotide natrium of proper amount of clear water dissolving, its solution adds alcohol and separates out, crystallization suction filtration is dry, approximately obtain 85-90 grams, white nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and a certain amount of helping and agent water soluble mixt, gains are pulverized, crossed 200 mesh sieves, mix with auxiliary agent (while again adding auxiliary agent).
Embodiment 19: by 100 grams of the crude product nucleic acid making from the mycelium of fermentation plant's by product by dry method or wet method, be dissolved in the potassium hydroxide solution of 2.5 liter of 6 N, under 40 DEG C of conditions, stir 10 hours, winner will refer to and contain: 5 '-adenylate dipotassium, 5 '-bird adenylate dipotassium, 5 '-cytidylic acid dipotassium, sylvite (the deoxyribonucleoside hydrochlorate in addition: 5 '-deoxyadenylic acid dipotassium of four kind 5 '-mixed nucleotides of 5 '-uridylic acid dipotassium, 5 '-deoxidation bird adenylate dipotassium, 5 '-deoxycytidylic acid dipotassium, 5 '-
Deoxyuridylic acid dipotassium,, the sylvite of five kind 5 '-deoxidation mixed nucleotides of 5 '-deoxythymidylic acid dipotassium and amino acid, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc.) and solution, filters, filtrate is neutralized to pH8-8.5, is evaporated to appropriate; Concentrate is added to activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into 20%; Dissolve nearly dry nucleotide potassium and deoxynucleotide potassium by proper amount of clear water, its solution adds alcohol separates out, and crystallization suction filtration is dry, approximately obtains 88-93 grams of white nucleotide sylvite and deoxyribonucleoside acid potassium salt and a certain amount of auxiliary agent and water soluble mixt.Gains are pulverized, crossed 200 mesh sieves, mix with auxiliary agent (while again adding auxiliary agent).
Embodiment 20: paddy rice: spray twice, for initial bloom stage (heading 10% left and right) is watered 30 liters with 20 grams of nutrients (containing glucose 80-85%'s), concentration 100ppm left and right, evenly sprays on one mu of paddy rice for the first time.Be full-bloom stage for the second time, within 5-6 days after spraying for the first time, be watered 30 liters with 20 grams of nutrients, evenly spray on one mu of paddy rice and get final product (70-100 kilograms of mu volume increase, volume increase 15% left and right).
Embodiment 21: cotton: spray twice, for the first time also can initial bloom stage in the flower bud phase, be watered 30 liters with 30 grams of nutrients, evenly spray on one mu of cotton.Full-bloom stage is watered 36 liters with 30 grams evenly spray on one mu of cotton can (mu volume increase be more than 7%) for the second time.
Embodiment 22: rape: spraying twice, is initial bloom stage for the first time, is watered 20 liters with 20 grams of nutrients and sprays on one mu of rape.Be full-bloom stage for the second time, be watered 30 liters with 20 grams, spray on one mu of rape, volume increase 15-20 kilogram.
Embodiment 23: tobacco: spray twice, with 30 grams, be often watered 30 kilograms, concentration 200ppm left and right at every turn, spray on one mu of tobacco leaf, spray and carry out 20-26 day in time in May, (the south north time is slightly different), sprays for the first time, 3-6 day in June sprays for the second time, concentration 150ppm left and right, quality improves a grade, volume increase 20% left and right.
Embodiment 24: tealeaves: at growth germination period, spray for the first time, spray once after every harvesting, concentration 180ppm left and right.
Embodiment 25: capsicum: at initial bloom stage and full-bloom stage respectively once, concentration 200-300ppm, mu volume increase 40%~80%.
Embodiment 26: strawberry: spray in the fruitlet phase, be watered 18 liters with 10 grams and spray, spray 3~4 times after harvesting in batches and can increase production 38%.
Embodiment 27: traditional Chinese medicine: the traditional Chinese medicine of different parts, using method differs, generally at initial bloom stage and full-bloom stage respectively once, concentration 100-260ppm.
Embodiment 28: mushroom: within every 3 days, spray once from fruiting, concentration 100-150ppm, volume increase 50-80%, shortens collecting time 1/3rd.