CN103351191B - Planting method for tea leaves - Google Patents
Planting method for tea leaves Download PDFInfo
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- CN103351191B CN103351191B CN201310249545.1A CN201310249545A CN103351191B CN 103351191 B CN103351191 B CN 103351191B CN 201310249545 A CN201310249545 A CN 201310249545A CN 103351191 B CN103351191 B CN 103351191B
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- China
- Prior art keywords
- hydrochlorate
- deoxynucleoside
- nucleosides
- nutrient substance
- nucleic acid
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- 238000000034 method Methods 0.000 title claims abstract description 38
- 241001122767 Theaceae Species 0.000 title claims abstract 7
- 235000015097 nutrients Nutrition 0.000 claims abstract description 56
- 239000002773 nucleotide Substances 0.000 claims abstract description 26
- 241000196324 Embryophyta Species 0.000 claims abstract description 20
- 230000012010 growth Effects 0.000 claims abstract description 13
- -1 nucleotide potassium salts Chemical class 0.000 claims abstract description 9
- 230000035784 germination Effects 0.000 claims abstract description 4
- 239000002777 nucleoside Substances 0.000 claims description 68
- 125000003835 nucleoside group Chemical group 0.000 claims description 67
- 239000000126 substance Substances 0.000 claims description 51
- 102000039446 nucleic acids Human genes 0.000 claims description 40
- 108020004707 nucleic acids Proteins 0.000 claims description 40
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 37
- 239000008103 glucose Substances 0.000 claims description 37
- 150000007523 nucleic acids Chemical class 0.000 claims description 37
- 125000003729 nucleotide group Chemical group 0.000 claims description 29
- 239000007921 spray Substances 0.000 claims description 23
- 239000002253 acid Substances 0.000 claims description 20
- 239000000243 solution Substances 0.000 claims description 18
- 238000000855 fermentation Methods 0.000 claims description 16
- 230000004151 fermentation Effects 0.000 claims description 16
- 238000004519 manufacturing process Methods 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 12
- 239000000706 filtrate Substances 0.000 claims description 12
- 238000002513 implantation Methods 0.000 claims description 12
- 238000000967 suction filtration Methods 0.000 claims description 11
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 9
- 238000002425 crystallisation Methods 0.000 claims description 9
- 230000008025 crystallization Effects 0.000 claims description 9
- 239000001103 potassium chloride Substances 0.000 claims description 9
- 235000011164 potassium chloride Nutrition 0.000 claims description 9
- 239000002994 raw material Substances 0.000 claims description 9
- 230000007062 hydrolysis Effects 0.000 claims description 8
- 238000006460 hydrolysis reaction Methods 0.000 claims description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 229930091371 Fructose Natural products 0.000 claims description 6
- 239000005715 Fructose Substances 0.000 claims description 6
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 6
- 229930006000 Sucrose Natural products 0.000 claims description 6
- 239000003513 alkali Substances 0.000 claims description 6
- 230000006837 decompression Effects 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims description 4
- 238000003306 harvesting Methods 0.000 claims description 4
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 claims description 4
- 239000002585 base Substances 0.000 claims description 3
- 150000007516 brønsted-lowry acids Chemical class 0.000 claims description 3
- 150000007528 brønsted-lowry bases Chemical class 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 239000012266 salt solution Substances 0.000 claims description 3
- 150000001413 amino acids Chemical class 0.000 abstract description 18
- 230000000694 effects Effects 0.000 abstract description 8
- 230000002411 adverse Effects 0.000 abstract description 3
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 3
- 230000002708 enhancing effect Effects 0.000 abstract description 2
- 230000008569 process Effects 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 231100000956 nontoxicity Toxicity 0.000 abstract 1
- 159000000001 potassium salts Chemical class 0.000 abstract 1
- 229910052700 potassium Inorganic materials 0.000 description 35
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 34
- 239000011591 potassium Substances 0.000 description 34
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 24
- 229950006790 adenosine phosphate Drugs 0.000 description 24
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 21
- 239000000203 mixture Substances 0.000 description 21
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 19
- 239000012752 auxiliary agent Substances 0.000 description 14
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 13
- UDMBCSSLTHHNCD-UHFFFAOYSA-N Coenzym Q(11) Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(O)=O)C(O)C1O UDMBCSSLTHHNCD-UHFFFAOYSA-N 0.000 description 12
- 230000008635 plant growth Effects 0.000 description 11
- 230000001737 promoting effect Effects 0.000 description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 8
- KCIDZIIHRGYJAE-YGFYJFDDSA-L dipotassium;[(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] phosphate Chemical compound [K+].[K+].OC[C@H]1O[C@H](OP([O-])([O-])=O)[C@H](O)[C@@H](O)[C@H]1O KCIDZIIHRGYJAE-YGFYJFDDSA-L 0.000 description 7
- 159000000000 sodium salts Chemical class 0.000 description 7
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 6
- 239000003337 fertilizer Substances 0.000 description 6
- 229910052698 phosphorus Inorganic materials 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 5
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- 239000011574 phosphorus Substances 0.000 description 5
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 4
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 4
- 229920000742 Cotton Polymers 0.000 description 4
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 4
- 240000007594 Oryza sativa Species 0.000 description 4
- 235000007164 Oryza sativa Nutrition 0.000 description 4
- IWUCXVSUMQZMFG-AFCXAGJDSA-N Ribavirin Chemical compound N1=C(C(=O)N)N=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](CO)O1 IWUCXVSUMQZMFG-AFCXAGJDSA-N 0.000 description 4
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 4
- 239000006227 byproduct Substances 0.000 description 4
- 229910052791 calcium Inorganic materials 0.000 description 4
- 239000011575 calcium Substances 0.000 description 4
- 229910052802 copper Inorganic materials 0.000 description 4
- 239000010949 copper Substances 0.000 description 4
- 229910052742 iron Inorganic materials 0.000 description 4
- 239000011777 magnesium Substances 0.000 description 4
- 229910052749 magnesium Inorganic materials 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 229960000329 ribavirin Drugs 0.000 description 4
- HZCAHMRRMINHDJ-DBRKOABJSA-N ribavirin Natural products O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1N=CN=C1 HZCAHMRRMINHDJ-DBRKOABJSA-N 0.000 description 4
- 235000009566 rice Nutrition 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 239000011701 zinc Substances 0.000 description 4
- 229910052725 zinc Inorganic materials 0.000 description 4
- WUBAOANSQGKRHF-XVFCMESISA-N 1-[(2r,3r,4s,5r)-3,4-dihydroxy-5-methyloxolan-2-yl]pyrimidine-2,4-dione Chemical compound O[C@@H]1[C@H](O)[C@@H](C)O[C@H]1N1C(=O)NC(=O)C=C1 WUBAOANSQGKRHF-XVFCMESISA-N 0.000 description 3
- UGUILUGCFSCUKR-GJMOJQLCSA-N 1-[(2r,4s,5r)-4-hydroxy-5-methyloxolan-2-yl]-5-methylpyrimidine-2,4-dione Chemical compound C1[C@H](O)[C@@H](C)O[C@H]1N1C(=O)NC(=O)C(C)=C1 UGUILUGCFSCUKR-GJMOJQLCSA-N 0.000 description 3
- UHDGCWIWMRVCDJ-UHFFFAOYSA-N 1-beta-D-Xylofuranosyl-NH-Cytosine Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(CO)O1 UHDGCWIWMRVCDJ-UHFFFAOYSA-N 0.000 description 3
- XGYIMTFOTBMPFP-KQYNXXCUSA-N 5'-deoxyadenosine Chemical compound O[C@@H]1[C@H](O)[C@@H](C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 XGYIMTFOTBMPFP-KQYNXXCUSA-N 0.000 description 3
- WDRISBUVHBMJEF-MROZADKFSA-N 5-deoxy-D-ribose Chemical compound C[C@@H](O)[C@@H](O)[C@@H](O)C=O WDRISBUVHBMJEF-MROZADKFSA-N 0.000 description 3
- UHDGCWIWMRVCDJ-PSQAKQOGSA-N Cytidine Natural products O=C1N=C(N)C=CN1[C@@H]1[C@@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-PSQAKQOGSA-N 0.000 description 3
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 3
- PNPLHKHBANFGMS-WFIJOQBCSA-N [Na].[Na].[C@@H]1([C@H](O)[C@H](O)[C@@H](COP(=O)(O)O)O1)N1C(=O)N=C(N)C=C1 Chemical compound [Na].[Na].[C@@H]1([C@H](O)[C@H](O)[C@@H](COP(=O)(O)O)O1)N1C(=O)N=C(N)C=C1 PNPLHKHBANFGMS-WFIJOQBCSA-N 0.000 description 3
- 239000012043 crude product Substances 0.000 description 3
- 239000007857 degradation product Substances 0.000 description 3
- KURVIXMFFSNONZ-WFIJOQBCSA-L disodium;[(2r,3s,4r,5r)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound [Na+].[Na+].O[C@@H]1[C@H](O)[C@@H](COP([O-])([O-])=O)O[C@H]1N1C(=O)NC(=O)C=C1 KURVIXMFFSNONZ-WFIJOQBCSA-L 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 229910052748 manganese Inorganic materials 0.000 description 3
- 239000011572 manganese Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 229940061671 uridine 5-mo-phos disod Drugs 0.000 description 3
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 244000061176 Nicotiana tabacum Species 0.000 description 2
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- 244000269722 Thea sinensis Species 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- NCMVOABPESMRCP-SHYZEUOFSA-N 2'-deoxycytosine 5'-monophosphate Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)C1 NCMVOABPESMRCP-SHYZEUOFSA-N 0.000 description 1
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- 240000008574 Capsicum frutescens Species 0.000 description 1
- MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 description 1
- NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 description 1
- 235000016623 Fragaria vesca Nutrition 0.000 description 1
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- 102000018997 Growth Hormone Human genes 0.000 description 1
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- 101710104378 Putative malate oxidoreductase [NAD] Proteins 0.000 description 1
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- 229940088710 antibiotic agent Drugs 0.000 description 1
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- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000019504 cigarettes Nutrition 0.000 description 1
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- JSRLJPSBLDHEIO-SHYZEUOFSA-N dUMP Chemical compound O1[C@H](COP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 JSRLJPSBLDHEIO-SHYZEUOFSA-N 0.000 description 1
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- 229940029575 guanosine Drugs 0.000 description 1
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- 150000002500 ions Chemical class 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
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- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 1
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Abstract
The invention discloses a good-quality and high-yield planting method for tea leaves. The planting method is characterized in that a nutrient is applied in a growth period of the tea leaves, wherein the nutrient mainly comprises the following components by weight percent: 10-30% of nucleotide potassium salts and deoxynucleotide potassium salts and 70-90% of sugar and/or amino acid; the time for applying the nutrient in the growth period are that the nutrient is applied for the first time in a growth germination period and is applied in each time when the tea leaves are picked. The good-quality and high-yield planting method has the advantages that the nutrient has no toxicity, harm, residue and any adverse side effect to the tea leaves; the nutrient is beneficial to increasing of the yield and the income and improving the efficiency and has good effects of enhancing the resistance and improving the quality for plants; the method has a simple process and low cost and is easy for large-scale production and promotion.
Description
The application is application number 201010514217.6, the applying date: on October 21st, 2010, what denomination of invention was " nutrient substance of a kind of Promoting plant growth and preparation method thereof " divides an application.
Technical field
The present invention relates to the implantation methods of a kind of implantation methods of tealeaves, particularly a kind of tealeaves.
Background technology
Greatly develop plant husbandry, forestry, promote that crop yield increases income, make our country green, it is the problem that various countries scientist pays close attention to research, the growth of plant be unable to do without and supplements the nutrients, and present plant husbandry, mainly by executing N,P,K fertilizers and organic fertilizer promotes crop growth, use too much fertilizer very large on the quality and flavor impact of farm crop, be unfavorable for development and food safety and the environmental protection of green agriculture.Therefore, people have studied growth hormone relevant to nucleic acid, nutrient substance, fertilizer.In theory: nucleic acid is the basic substance that forms life, is the basis of substance metabolism and energy metabolism, and the character of nucleic acid and the difference of quantity can cause the diversity of biological species and growth conditions.For congener life entity, the quantity of nucleic acid and the difference of existence form have determined prosperous the declining of power of this life entity.Therefore, nucleic acid substances has irreplaceable effect for the output raising in farm crop and even the production of whole vegitabilia and product quality improvement; Particularly several nucleotides salt and deoxynucleoside hydrochlorate and the cooperations thereof of nucleic acid small molecules such as nucleosides, Nucleotide, pyrimidine and purine, the not only growth of regulating plant very effectively, can be used as again nutrition source is absorbed rapidly by plant, strengthen plant root vigor, improve plant anti-adversity ability, thus improve crop output, improve product quality.Nucleic acid small molecules has enhancing improving activity of root system to plant, improves the absorption of root to phosphorus, potash fertilizer; Improve the activity of farm crop malic enzyme and succsinic acid enzyme, promote the assimilative capacity of plant, strengthen photosynthetic intensity, to plant-growth have regulate and trophism (Wang Xi etc. the research [J] of the application and function mechanism of nucleic acid degradation product in Rice Production. Acta Biochimica et Biophysica Sinica, 1975,7 (1): 31-39).Chinese patent literature (publication number: 101186534) disclose a kind of preparation method who contains nucleic acid degradation product foliage fertilizer, concrete step is: 1., lixiviate nuclease, 2., dissolve Yeast Nucleic Acid (alkali lye hydrotropy), 3., the hydrolysis of nucleic acid, must contain nucleosides, Nucleotide, purine, the multiple nucleic acids small molecules such as pyrimidine and a small amount of reducing sugar and few large, in, trace element, the method be take Yeast Nucleic Acid as raw material, with enzymatic hydrolysis, exist production cost high, degrade not thorough, few containing deoxynucleotide composition, in mixed system, Nucleotide salt yield is low, the mutual mixing ratio imbalance of various nucleosides hydrochlorates and deoxynucleotide salt face, very large on result of use impact.Chinese patent literature (publication number: 101050143) disclose a kind of nutrient substance that promotes crop growth, it is comprised of ribavirin and glucose, production and the guanosine of ribavirin are in close relations, and this patent exists ribavirin high cost, the unconspicuous problem of practical effect; Chinese patent literature (publication number: the preparation method who 1238323) discloses a kind of nucleic acid foliage fertilizer, the degraded of its nucleic acid adopts the method for alkaline hydrolysis, degradation product is purine and pyrimidine, there is equally nucleic acid high cost in this patent, various nucleosides hydrochlorates and the mutual mixing ratio of deoxynucleotide salt face are unreasonable, affect the problem of result of use.
Summary of the invention
The implantation methods that the object of this invention is to provide a kind of tealeaves, its production cost is low, and method is simple, is easy to large-scale production and popularization; In mixed system, Nucleotide salt yield is high, and various nucleosides hydrochlorates and the mutual mixing ratio of deoxynucleotide salt face are reasonable, nontoxic, harmless, noresidue, without any adverse side effect; Increase production, increase income, synergy, strengthen resistance, improve quality effective;
The present invention is achieved in that
The implantation methods of tealeaves, is characterized in that: during tea growth, use nutrient substance; Described nutrient substance is mainly comprised of nucleosides hydrochlorate and deoxynucleoside hydrochlorate, sugar, its weight percent is: nucleosides hydrochlorate and deoxynucleoside hydrochlorate 10-30%, sugar 70-90%, the nucleosides hydrochlorate in described nutrient substance and deoxynucleoside hydrochlorate are that to adopt the nucleic acid of dry method or wet production be raw material for the mycelium of take after fermentation plant fermentation:
1., the hydrolysis of the nucleic acid ratio mixed nucleus bronsted lowry acids and bases bronsted lowry liquid that is 1:20-30 according to nucleic acid, buck weight ratio, alkali lye is
5.5-7N, under 30-45 ℃ of condition, stirs 10-14 hours, obtains nucleosides hydrochlorate and deoxynucleoside acid salt solution, filters, and filtrate is adjusted pH8-8.5, concentrating under reduced pressure;
2., decolouring, dry concentrated solution is added to activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 10-25%;
3., clear water dissolves nearly dry nucleosides hydrochlorate and deoxynucleoside hydrochlorate for crystallization, its solution adds alcohol separates out, and crystallization is taken out
Be filtered dry dry, the white nucleosides hydrochlorate and deoxynucleoside hydrochlorate and the water soluble mixt that obtain, time and concentration that described growing period is used nutrient substance are: growth germination period spray once, after every harvesting, spray once concentration 180ppm.
Described nucleosides hydrochlorate and deoxynucleoside hydrochlorate are Nucleotide sylvite and deoxynucleoside acid potassium salt.
Sugar in described nutrient substance is glucose or sucrose or fructose.
Described nutrient substance is 100 grams of the crude product nucleic acid that make from the mycelium of fermentation plant's byproduct by dry method or wet method, be dissolved in the sodium hydroxide solution of 3 liters of 6.3N, under 37 ℃ of conditions, stir 12 hours, winner will refer to and contain: 5 '-adenylic acid (AMP) disodium, 5 '-bird adenylic acid (AMP) disodium, 5 '-cytidylic acid disodium, 5 '-uridine monophosphate disodium, the sodium salt of four kind 5 '-mixed nucleotides, also has deoxynucleoside hydrochlorate: 5 '-Desoxyadenosine acid disodium, 5 '-deoxidation bird adenylic acid (AMP) disodium, 5 '-Deoxyribose cytidine acid disodium, 5 '-deoxyuridine acid disodium, , 5 '-deoxythymidine acid disodium, sodium salt and the impurity of five kind 5 '-deoxidation mixed nucleotides: amino acid, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, the solution of manganese, filter, filtrate is neutralized to pH8-8.5, be evaporated to appropriate, concentrated solution is added to activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 15%, by proper amount of clear water, dissolve nearly dry new-nucleo and Sodium Deoxyribomuleotide, its solution adds alcohol and separates out, crystallization suction filtration is dry, obtain 90 grams, white nucleosides hydrochlorate and deoxynucleoside hydrochlorate and a certain amount of water soluble mixt, gains are pulverized, crossed 200 mesh sieves, mix with sugar or amino acid the nutrient substance of evenly making.
?the implantation methods of tealeaves: with the nutrient substance spraying concentration after water dissolution be at every turn: 180ppm left and right.
The implantation methods of tealeaves: nucleosides hydrochlorate and deoxynucleoside hydrochlorate are Nucleotide sylvite and deoxynucleoside acid potassium salt.
The implantation methods of tealeaves: the sugar in described nutrient substance is glucose or sucrose or fructose.
The implantation methods of tealeaves: the sugar in described nutrient substance is glucose or sucrose or fructose.
The implantation methods of tealeaves: in the nucleosides hydrochlorate and deoxynucleoside hydrochlorate of described nutrient substance, weight percent is: nucleosides hydrochlorate 85-99%, deoxynucleoside hydrochlorate 1-15%.
The implantation methods of tealeaves: in the nucleosides hydrochlorate and deoxynucleoside hydrochlorate of described nutrient substance, weight percent is: nucleosides hydrochlorate 85-99%, deoxynucleoside hydrochlorate 1-15%.
The nutrient substance of Promoting plant growth, it is mainly comprised of nucleosides hydrochlorate and deoxynucleoside hydrochlorate, auxiliary agent, and its weight percent is: nucleosides hydrochlorate and deoxynucleoside hydrochlorate 10-80%, auxiliary agent 20-90%.
The nutrient substance of Promoting plant growth, in nucleosides hydrochlorate and deoxynucleoside hydrochlorate, weight percent is: nucleosides hydrochlorate 85-99%, deoxynucleoside hydrochlorate 1-15%.
The nutrient substance of Promoting plant growth, in nucleosides hydrochlorate and deoxynucleoside hydrochlorate, weight percent is: nucleosides hydrochlorate 88-96%, deoxynucleoside hydrochlorate 4-12%.
The nutrient substance of Promoting plant growth, nucleosides hydrochlorate and deoxynucleoside hydrochlorate and auxiliary agent, weight percent is:
Nucleosides hydrochlorate and deoxynucleoside hydrochlorate 10-30%, auxiliary agent 70-90%.
The nutrient substance of Promoting plant growth, auxiliary agent is sugar or amino acid.
The nutrient substance of Promoting plant growth, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are sodium salt or sylvite.
The preparation method of the nutrient substance of Promoting plant growth provided by the present invention, concrete steps are as follows: take nucleic acid as raw material, is mainly that to adopt the nucleic acid of dry method or wet production be raw material for mycelium from fermentation plant fermentation:
1., the hydrolysis of the nucleic acid ratio mixed nucleus bronsted lowry acids and bases bronsted lowry liquid that is 1:20-30 according to nucleic acid, buck weight ratio, alkali lye is 5.5-7N, under 30-45 ℃ of condition, stir 10-14 hours, obtain nucleosides hydrochlorate and deoxynucleoside acid salt solution, filter, filtrate is adjusted pH8-8.5, concentrating under reduced pressure;
2., decolouring, dry concentrated solution is added to activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 10-25%;
3., crystallization dissolves nucleosides hydrochlorate and the deoxynucleoside hydrochlorate after dry with clear water, its solution adds alcohol separates out, and crystallization suction filtration is dry, obtains white nucleosides hydrochlorate and deoxynucleoside hydrochlorate and water soluble mixt.
4., nucleosides hydrochlorate and deoxynucleoside hydrochlorate and water soluble mixt are pulverized, cross 200 mesh sieves and mix with auxiliary agent again.
In nucleic acid hydrolysis process described in this preparation method, alkali lye used is sodium hydroxide or potassium hydroxide solution.
In the present invention, nucleosides hydrochlorate mainly refers to: 5 '-adenylic acid (AMP) disodium or potassium, 5 '-bird adenylic acid (AMP) disodium or potassium, 5 '-cytidylic acid disodium or potassium, sodium salt or the sylvite of four kind 5 '-mixed nucleotides of 5 '-uridine monophosphate disodium or potassium; Deoxynucleoside hydrochlorate refers to: 5 '-Desoxyadenosine acid disodium or potassium, 5 '-deoxidation bird adenylic acid (AMP) disodium or potassium, 5 '-Deoxyribose cytidine acid disodium or potassium, 5 '-deoxyuridine acid disodium,, sodium salt or the sylvite of five kind 5 '-deoxidation mixed nucleotides of 5 '-deoxythymidine acid disodium or potassium.The source of nucleosides hydrochlorate and deoxynucleoside hydrochlorate is for take nucleic acid as raw material, be mainly mycelium from fermentation plant fermentation to adopt the nucleic acid of dry method or wet production be raw material, this kind of nucleic acid be take Yeast Nucleic Acid (RNA) as a small amount of deoxidation core of main mixing
Ribosomal ribonucleic acid (DNA), makes with alkali lye hydrolysis; Auxiliary agent is mainly derived from two aspects: 1, the nucleic acid of dry method or wet production also contains the impurity such as sugar, protein, various metals or nonmetallic ion, has amino acid, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc. after hydrolysis; 2, exogenous adding: sugar (reducing sugar, non-reducing sugar: as glucose, sucrose also have fructose etc.), amino acid, big or middle, micro-etc.
The present invention adopt mainly by nucleosides hydrochlorate and deoxynucleoside hydrochlorate, the Promoting plant growth nutrient substance that auxiliary agent forms and for take nucleic acid as raw material, mainly from fermentation plant (Gourmet Powder Factory, brew-house, antibiotics plant, yeast factory, Amino Acid Factory, paper mill, active sludge after sewage disposal, petroleum fermentations etc.) nucleic acid of the employing dry method of byproduct (tankage) mycelium after fermentation or wet production is the preparation method of raw material hydrolysis, with currently available products and the method (product that adds ribavirin, the product being directly hydrolyzed with Yeast Nucleic Acid, enzyme solution) compare and have the following advantages: 1, it is low that mycelium from fermentation plant fermentation is produced its production cost of Yeast Nucleic Acid, be easy to large-scale production and popularization, 2, the nucleic acid that mycelium is produced is containing useful impurity, in addition basic hydrolysis method, technique is simple, in mixed system, Nucleotide salt yield is high, and various nucleosides hydrochlorates and the mutual mixing ratio of deoxynucleotide salt face are reasonable, and amino acid, sugar part and other organic-inorganic auxiliary agent content are higher, therefore, increasing both production and income synergy, strengthens resistance, improves quality effective, 3,, nontoxic, harmless, noresidue, without any adverse side effect, 4, be widely used in grain, cotton, oil, cigarette, tea, really, plant and the bacterium mushroom series products such as dish, Chinese medicinal materials, flowers, forest.
Embodiment
Embodiment 1: get 1 gram of nucleosides hydrochlorate and deoxynucleoside hydrochlorate, and 6 grams of glucose, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 2: get 1 gram of nucleosides hydrochlorate and deoxynucleoside hydrochlorate, and 2 grams of sucrose, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 3: get 2 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, 8 grams of glucose, nucleosides hydrochlorate and de-
Oxygen nucleosides hydrochlorate is pulverized, and mixes with glucose, obtains nutrient substance.
Embodiment 4: get 1.5 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, and 8.5 grams of glucose, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 5: get 1 gram of nucleosides hydrochlorate and deoxynucleoside hydrochlorate, and 9 grams of glucose, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 6: get 2.5 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, and 7.5 grams of glucose, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 7: get 4 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, and 6 grams, fructose, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 8: get 5 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, and 5 grams of glucose, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 9: get 1 gram, Nucleotide potassium and deoxynucleotide potassium, and 9 grams of glucose, Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 10: get 1.5 grams, Nucleotide potassium and deoxynucleotide potassium, and 8.5 grams of glucose, Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 11: get 2 grams, Nucleotide potassium and deoxynucleotide potassium, and 8 grams of glucose, Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 12: get 2.5 grams, Nucleotide potassium and deoxynucleotide potassium, and 7.5 grams of glucose, Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 13: get 3 grams, Nucleotide potassium and deoxynucleotide potassium, and 7 grams of glucose, Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 14: get 1.5 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, and 4.5 grams of glucose, 4 grams, amino acid, nucleosides hydrochlorate and deoxynucleoside hydrochlorate and amino acid are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 15: get 2 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, and 4 grams of glucose, 4 grams, amino acid, nucleosides hydrochlorate and deoxynucleoside hydrochlorate and amino acid are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 16: get 3 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, and 4 grams of glucose, 3 grams, amino acid, nucleosides hydrochlorate and deoxynucleoside hydrochlorate and amino acid are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 17: get 2 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, and 8 grams, amino acid, nucleosides hydrochlorate and deoxynucleoside hydrochlorate and amino acid are pulverized, and mix, and obtain nutrient substance.
Embodiment 18: by 100 grams of the crude product nucleic acid making from the mycelium of fermentation plant's byproduct by dry method or wet method, be dissolved in the sodium hydroxide solution of 3 liters of 6.3N, under 37 ℃ of conditions, stir 12 hours, winner will refer to and contain: 5 '-adenylic acid (AMP) disodium, 5 '-bird adenylic acid (AMP) disodium, 5 '-cytidylic acid disodium, the sodium salt of four kind 5 '-mixed nucleotides of 5 '-uridine monophosphate disodium (also has deoxynucleoside hydrochlorate: 5 '-Desoxyadenosine acid disodium, 5 '-deoxidation bird adenylic acid (AMP) disodium, 5 '-Deoxyribose cytidine acid disodium, 5 '-deoxyuridine acid disodium, , sodium salt and the amino acid of five kind 5 '-deoxidation mixed nucleotides of 5 '-deoxythymidine acid disodium, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc.) solution, filter, filtrate is neutralized to pH8-8.5, be evaporated to appropriate, concentrated solution is added to activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 15%, by proper amount of clear water, dissolve nearly dry new-nucleo and Sodium Deoxyribomuleotide, its solution adds alcohol and separates out, crystallization suction filtration is dry, approximately obtain 85-90 grams, white nucleosides hydrochlorate and deoxynucleoside hydrochlorate and a certain amount of helping and agent water soluble mixt, gains are pulverized, crossed 200 mesh sieves, mix with auxiliary agent (while again adding auxiliary agent).
Embodiment 19: by 100 grams of the crude product nucleic acid making from the mycelium of fermentation plant's byproduct by dry method or wet method, be dissolved in the potassium hydroxide solution of 2.5 liter of 6 N, under 40 ℃ of conditions, stir 10 hours, winner will refer to and contain: 5 '-adenylic acid (AMP) dipotassium, 5 '-bird adenylic acid (AMP) dipotassium, 5 '-cytidylic acid dipotassium, the sylvite of four kind 5 '-mixed nucleotides of 5 '-uridylic acid dipotassium (also has deoxynucleoside hydrochlorate: 5 '-deoxyadenylic acid dipotassium, 5 '-deoxidation bird adenylic acid (AMP) dipotassium, 5 '-deoxycytidylic acid(dCMP) dipotassium, 5 '-
Deoxyuridylic acid dipotassium,, the sylvite of five kind 5 '-deoxidation mixed nucleotides of 5 '-deoxythymidylic acid dipotassium and amino acid, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc.) and solution, filters, filtrate is neutralized to pH8-8.5, is evaporated to appropriate; Concentrated solution is added to activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into 20%; By proper amount of clear water, dissolve nearly dry Nucleotide potassium and deoxynucleotide potassium, its solution adds alcohol separates out, and crystallization suction filtration is dry, approximately obtains 88-93 grams of white Nucleotide sylvite and deoxynucleoside acid potassium salt and a certain amount of auxiliary agent and water soluble mixt.Gains are pulverized, crossed 200 mesh sieves, mix with auxiliary agent (while again adding auxiliary agent).
Embodiment 20: paddy rice: spray twice, for initial bloom stage (heading 10% left and right) is watered 30 liters with 20 grams of nutrient substances (containing glucose 80-85%'s), concentration 100ppm left and right, evenly sprays on one mu of paddy rice for the first time.Be full-bloom stage for the second time, within 5-6 days after spraying for the first time, with 20 grams of nutrient substances, be watered 30 liters, evenly spray on one mu of paddy rice and get final product (70-100 kilograms of mu volume increase, volume increase 15% left and right).
Embodiment 21: cotton: spray twice, for the first time also can initial bloom stage in the flower bud phase, and with 30 grams of nutrient substances, be watered 30 liters, evenly spray on one mu of cotton.Full-bloom stage is watered 36 liters with 30 grams evenly spray on one mu of cotton can (mu volume increase be more than 7%) for the second time.
Embodiment 22: rape: spraying twice, is initial bloom stage for the first time, be watered 20 liters spray on one mu of rape with 20 grams of nutrient substances.Be full-bloom stage for the second time, be watered 30 liters with 20 grams, spray on one mu of rape, volume increase 15-20 kilogram.
Embodiment 23: tobacco: spray twice, with 30 grams, be often watered 30 kilograms, concentration 200ppm left and right at every turn, spray on one mu of tobacco leaf, spray and carry out 20-26 day in time in May, (the south north time is slightly different), sprays for the first time, 3-6 day in June sprays for the second time, concentration 150ppm left and right, quality improves a grade, volume increase 20% left and right.
Embodiment 24: tealeaves: at growth germination period, spray for the first time, after every harvesting, spray once, concentration 180ppm left and right.
Embodiment 25: capsicum: initial bloom stage and full-bloom stage each once, concentration 200-300ppm, mu volume increase 40%～80%.
Embodiment 26: strawberry: in the fruitlet phase, spray, be watered 18 liters spray with 10 grams, spray 3～4 times in batches and can increase production 38% after harvesting.
Embodiment 27: Chinese medicinal materials: the Chinese medicinal materials of different sites, using method differs, generally initial bloom stage and full-bloom stage each once, concentration 100-260ppm.
Embodiment 28: mushroom: from fruiting, within every 3 days, spray once, and concentration 100-150ppm, volume increase 50-80%, shortens collecting time 1/3rd.
Claims (1)
1. the implantation methods of tealeaves, is characterized in that: during tea growth, use nutrient substance; Described nutrient substance is mainly comprised of nucleosides hydrochlorate and deoxynucleoside hydrochlorate, sugar, its weight percent is: nucleosides hydrochlorate and deoxynucleoside hydrochlorate 10-30%, sugar 70-90%, the nucleosides hydrochlorate in described nutrient substance and deoxynucleoside hydrochlorate are that to adopt the nucleic acid of dry method or wet production be raw material for the mycelium of take after fermentation plant fermentation:
1., the hydrolysis of the nucleic acid ratio mixed nucleus bronsted lowry acids and bases bronsted lowry liquid that is 1:20-30 according to nucleic acid, buck weight ratio, alkali lye is 5.5-7N, under 30-45 ℃ of condition, stir 10-14 hours, obtain nucleosides hydrochlorate and deoxynucleoside acid salt solution, filter, filtrate is adjusted pH8-8.5, concentrating under reduced pressure;
2., decolouring, dry concentrated solution is added to activated carbon decolorizing, suction filtration, filtrate decompression is concentrated into moisture 10-25%;
3., crystallization is dissolved nucleosides hydrochlorate and the deoxynucleoside hydrochlorate after doing with clear water, its solution adds alcohol and separates out, crystallization suction filtration is dry, the white nucleosides hydrochlorate and deoxynucleoside hydrochlorate and the water soluble mixt that obtain, time and the concentration of during described tea growth, using nutrient substance are: at growth germination period, spray once, after every harvesting, spray once concentration 180ppm;
Described nucleosides hydrochlorate and deoxynucleoside hydrochlorate are Nucleotide sylvite and deoxynucleoside acid potassium salt;
Sugar in described nutrient substance is glucose or sucrose or fructose.
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| CN102146005B (en) * | 2010-11-25 | 2014-12-10 | 高旌 | Application method of auxin |
| CN103782778A (en) * | 2014-02-13 | 2014-05-14 | 常熟市虞山绿茶有限公司 | Tea planting method |
| CN105777242A (en) * | 2014-12-22 | 2016-07-20 | 重庆市万盛区盛泉茶厂 | Planting method of Xiuya |
| CN105766255A (en) * | 2014-12-22 | 2016-07-20 | 重庆市万盛区盛泉茶厂 | Method for planting high-mountain tea trees |
| CN114009269A (en) * | 2021-11-09 | 2022-02-08 | 贵州省黔桂茶业有限公司 | Planting method for improving quality and increasing yield of tea |
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| AU2007200687A1 (en) * | 2006-02-23 | 2007-09-06 | Van Schaik's Bio Gro Pty Ltd | A seagrass based nutrient product for plant biological growth stimulation and a method for preparing the same |
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