CN103348867B - Planting method of mushrooms - Google Patents
Planting method of mushrooms Download PDFInfo
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- CN103348867B CN103348867B CN201310249547.0A CN201310249547A CN103348867B CN 103348867 B CN103348867 B CN 103348867B CN 201310249547 A CN201310249547 A CN 201310249547A CN 103348867 B CN103348867 B CN 103348867B
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- Prior art keywords
- hydrochlorate
- nutrient
- deoxyribonucleoside
- grams
- nucleosides
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- 238000000034 method Methods 0.000 title claims abstract description 36
- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 23
- 235000015097 nutrients Nutrition 0.000 claims abstract description 65
- 150000001413 amino acids Chemical class 0.000 claims abstract description 30
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 29
- 230000012010 growth Effects 0.000 claims abstract description 9
- 239000002777 nucleoside Substances 0.000 claims description 80
- 125000003835 nucleoside group Chemical group 0.000 claims description 79
- 239000005549 deoxyribonucleoside Substances 0.000 claims description 76
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 55
- 239000008103 glucose Substances 0.000 claims description 55
- 229910052700 potassium Inorganic materials 0.000 claims description 42
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 41
- 239000011591 potassium Substances 0.000 claims description 41
- 239000002773 nucleotide Substances 0.000 claims description 25
- 239000007921 spray Substances 0.000 claims description 21
- 238000002513 implantation Methods 0.000 claims description 15
- 159000000000 sodium salts Chemical class 0.000 claims description 9
- 239000005715 Fructose Substances 0.000 claims description 5
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- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 5
- 229930006000 Sucrose Natural products 0.000 claims description 5
- 239000005720 sucrose Substances 0.000 claims description 5
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 8
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- 230000002411 adverse Effects 0.000 abstract description 3
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 3
- 231100000252 nontoxic Toxicity 0.000 abstract description 3
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- 239000000203 mixture Substances 0.000 description 32
- 102000039446 nucleic acids Human genes 0.000 description 26
- 108020004707 nucleic acids Proteins 0.000 description 26
- 150000007523 nucleic acids Chemical class 0.000 description 26
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 20
- 241000196324 Embryophyta Species 0.000 description 17
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- IWUCXVSUMQZMFG-AFCXAGJDSA-N Ribavirin Chemical compound N1=C(C(=O)N)N=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](CO)O1 IWUCXVSUMQZMFG-AFCXAGJDSA-N 0.000 description 4
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- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 3
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- 239000011572 manganese Substances 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
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- 229910052725 zinc Inorganic materials 0.000 description 3
- MXFFCFOTFSPXOE-JXOAFFINSA-N 1-[(2r,3r,4s,5r)-3,4-dihydroxy-5-methyloxolan-2-yl]-5-methylpyrimidine-2,4-dione Chemical compound O[C@@H]1[C@H](O)[C@@H](C)O[C@H]1N1C(=O)NC(=O)C(C)=C1 MXFFCFOTFSPXOE-JXOAFFINSA-N 0.000 description 2
- HLPAJQITBMEOML-XVFCMESISA-N 4-amino-1-[(2r,3r,4s,5r)-3,4-dihydroxy-5-methyloxolan-2-yl]pyrimidin-2-one Chemical compound O[C@@H]1[C@H](O)[C@@H](C)O[C@H]1N1C(=O)N=C(N)C=C1 HLPAJQITBMEOML-XVFCMESISA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 2
- 244000061176 Nicotiana tabacum Species 0.000 description 2
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- PNPLHKHBANFGMS-WFIJOQBCSA-N [Na].[Na].[C@@H]1([C@H](O)[C@H](O)[C@@H](COP(=O)(O)O)O1)N1C(=O)N=C(N)C=C1 Chemical compound [Na].[Na].[C@@H]1([C@H](O)[C@H](O)[C@@H](COP(=O)(O)O)O1)N1C(=O)N=C(N)C=C1 PNPLHKHBANFGMS-WFIJOQBCSA-N 0.000 description 2
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- KURVIXMFFSNONZ-WFIJOQBCSA-L disodium;[(2r,3s,4r,5r)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound [Na+].[Na+].O[C@@H]1[C@H](O)[C@@H](COP([O-])([O-])=O)O[C@H]1N1C(=O)NC(=O)C=C1 KURVIXMFFSNONZ-WFIJOQBCSA-L 0.000 description 2
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- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 2
- 229940061671 uridine 5-mo-phos disod Drugs 0.000 description 2
- 235000002566 Capsicum Nutrition 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 description 1
- NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 description 1
- 235000016623 Fragaria vesca Nutrition 0.000 description 1
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- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 description 1
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- WIXIBOVGGNRVQH-WFIJOQBCSA-N [K].[K].[C@@H]1([C@H](O)[C@H](O)[C@@H](COP(=O)(O)O)O1)N1C(=O)N=C(N)C=C1 Chemical compound [K].[K].[C@@H]1([C@H](O)[C@H](O)[C@@H](COP(=O)(O)O)O1)N1C(=O)N=C(N)C=C1 WIXIBOVGGNRVQH-WFIJOQBCSA-N 0.000 description 1
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Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Saccharide Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a planting method of mushrooms with good quality and high yield. The method comprises the steps as follows: nutrient is applied during the mushroom growth period; the nutrient mainly comprises components in percentage by weight as follows: 10%-80% of nucleotide salt and deoxynucleotide salt and 20%-90% of sugar or/and amino acid; and the time for applying the nutrient during the growth period is that the nutrient is sprayed once every three days during fruiting, and the concentration of the applied nutrient is 100-150 ppm. The planting method has the advantages that the nutrient is non-toxic and harmless, and has no residue and no adverse side effect on mushrooms; yield, income and efficiency are increased, resistance is enhanced, and an effect of improving quality is good; and further, the method is simple, low in cost and prone to large-scale production and popularization.
Description
The application is application number 201010514217.6, the applying date: on October 21st, 2010, what denomination of invention was " nutrient of a kind of Promoting plant growth and preparation method thereof " divides an application.
Technical field
The present invention relates to the implantation methods of the implantation methods, particularly a kind of mushroom of a kind of bacterium mushroom class plant.
Background technology
Greatly develop plant husbandry, forestry, promote that crop yield increases income, make our country green, it is the problem that various countries scientist pays close attention to research, the growth of plant be unable to do without and supplements the nutrients, and present plant husbandry, mainly by executing N,P,K fertilizers and organic manure promotes crop growth, use too much fertilizer very large on the quality and flavor impact of crops, be unfavorable for development and food security and the environmental protection of green agriculture.Therefore, people have studied growth hormone relevant to nucleic acid, nutrient, fertilizer.In theory: nucleic acid is the basic substance that forms life, is the basis of metabolism and energy metabolism, and the character of nucleic acid and the difference of quantity can cause the diversity of biological species and growth conditions.For congener life entity, the quantity of nucleic acid and the difference of existence form have determined prosperous the declining of power of this life entity.Therefore, nucleic acid substances has irreplaceable effect for the output raising in crops and even the production of whole vegetative kingdom and product quality improvement; Particularly several nucleotides salt and deoxyribonucleoside hydrochlorate and the cooperations thereof of the little molecule of nucleic acid such as nucleosides, nucleotide, pyrimidine and purine, the not only growth of regulating plant very effectively, can be used as again nutrient source is absorbed rapidly by plant, strengthen plant root vigor, improve plant anti-adversity ability, thus improve crop output, improve product quality.The little molecule of nucleic acid has enhancing improving activity of root system to plant, improves the absorption of root to phosphorus, potash fertilizer; Improve the activity of crops malate dehydrogenase and succinic acid enzyme, promote the assimilational capacity of plant, strengthen photosynthetic intensity, to plant growth have regulate and trophism (Wang Xi etc. the research [J] of the application and function mechanism of nucleic acid degradation product in Rice Production. Acta Biochimica et Biophysica Sinica, 1975,7 (1): 31-39).Chinese patent literature (publication number: 101186534) disclose a kind of preparation method who contains nucleic acid degradation product foliage fertilizer, concrete step is: 1., lixiviate nuclease, 2., dissolve ribonucleic acid (alkali lye hydrotropy), 3., the hydrolysis of nucleic acid, must contain
There are the little molecules of multiple nucleic acids such as nucleosides, nucleotide, purine, pyrimidine and a small amount of reducing sugar and few big or middle, trace element, the method be take ribonucleic acid as raw material, with enzymatic hydrolysis, exist production cost high, degrade not thorough, seldom, containing deoxynucleotide composition, in mixed system, nucleotide salt yield is low, the mutual mixing ratio imbalance of various nucleosides hydrochlorates and deoxynucleotide salt face is very large on result of use impact.Chinese patent literature (publication number: 101050143) disclose a kind of nutrient that promotes crop growth, it is comprised of Ribavirin and glucose, production and the guanosine of Ribavirin are in close relations, and this patent exists Ribavirin high cost, the unconspicuous problem of practical effect; Chinese patent literature (publication number: the preparation method who 1238323) discloses a kind of nucleic acid foliage fertilizer, the degraded of its nucleic acid adopts the method for alkaline hydrolysis, degradation product is purine and pyrimidine, there is equally nucleic acid high cost in this patent, various nucleosides hydrochlorates and the mutual mixing ratio of deoxynucleotide salt face are unreasonable, affect the problem of result of use.
Summary of the invention
The implantation methods that the object of this invention is to provide a kind of mushroom, its production cost is low, and method is simple, is easy to large-scale production and popularization; In mixed system, nucleotide salt yield is high, and various nucleosides hydrochlorates and the mutual mixing ratio of deoxynucleotide salt face are reasonable, nontoxic, harmless, noresidue, without any adverse side effect; Increase production, increase income, synergy, strengthen resistance, improve quality effective.
The present invention is achieved in that
The good quality and high output implantation methods of mushroom, during mushroom growth, uses nutrient.
Described nutrient is mainly comprised of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, sugar or amino acid, its percentage by weight is: nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 10-80%, sugar or amino acid 20-90%, the time that described growing period is used nutrient is: from fruiting, within every 3 days, spray once, the concentration of using nutrient is 100-150ppm.
The good quality and high output implantation methods of mushroom, the nucleosides hydrochlorate in described nutrient and deoxyribonucleoside hydrochlorate and sugar or amino acid, its percentage by weight is: nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 10-30%, sugar or amino acid 70-90%.
The good quality and high output implantation methods of mushroom, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are nucleotide potassium or sodium salt and deoxynucleotide potassium or sodium salt.
The good quality and high output implantation methods of mushroom, is characterized in that: the sugar in described nutrient is glucose or sucrose or fructose.
The good quality and high output implantation methods of mushroom, in the nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate of described nutrient, percentage by weight is: nucleosides hydrochlorate 85-99%, deoxyribonucleoside hydrochlorate 1-15%.
The implantation methods of mushroom: described nutrient refers to: get 2 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, 4 grams of glucose, 4 grams, amino acid, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix the nutrient made from glucose; Or refer to: get 3 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, 4 grams of glucose, 3 grams, amino acid, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix the nutrient made from glucose; Or refer to: get 2 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, 8 grams, amino acid, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix the nutrient of making; Or refer to: get 1 gram of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, 6 grams of glucose, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix the nutrient made from glucose; Or refer to: get 1 gram of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, 2 grams of sucrose, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix the nutrient made from glucose; Or refer to: get 2 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, 8 grams of glucose, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix the nutrient made from glucose; Or refer to: get 1.5 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, 8.5 grams of glucose, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix the nutrient made from glucose; Or refer to: get 1 gram of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, 9 grams of glucose, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix the nutrient made from glucose; Or refer to: get 2.5 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, 7.5 grams of glucose, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix the nutrient made from glucose; Or refer to: get 4 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, 6 grams, fructose, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix the nutrient made from glucose; Or refer to: get 5 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, 5 grams of glucose, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix the nutrient made from glucose; Or refer to: get 1 gram, nucleotide potassium and deoxynucleotide potassium, 9 grams of glucose, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix the nutrient made from glucose.
The nutrient of Promoting plant growth, in nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, percentage by weight is: nucleosides hydrochlorate 88-96%, deoxyribonucleoside hydrochlorate 4-12%.
The nutrient of Promoting plant growth, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and auxiliary agent, percentage by weight is:
Nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 10-30%, auxiliary agent 70-90%.
The nutrient of Promoting plant growth, auxiliary agent is sugar or amino acid.
The nutrient of Promoting plant growth, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are sodium salt or sylvite.
The preparation method of the nutrient of Promoting plant growth provided by the present invention, concrete steps are as follows: take nucleic acid as raw material, is mainly that to adopt the nucleic acid of dry method or wet production be raw material for mycelium from fermentation plant fermentation:
1., the hydrolysis of the nucleic acid ratio mixed nucleus bronsted lowry acids and bases bronsted lowry liquid that is 1:20-30 according to nucleic acid, buck weight ratio, alkali lye is 5.5-7N, under 30-45 ℃ of condition, stir 10-14 hours, obtain nucleosides hydrochlorate and deoxyribonucleoside acid salt solution, filter, filtrate is adjusted pH8-8.5, reduced pressure concentration;
2., decolouring, dry concentrate is added to activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 10-25%;
3., crystallization dissolves nucleosides hydrochlorate and the deoxyribonucleoside hydrochlorate after dry with clear water, its solution adds alcohol separates out, and crystallization suction filtration is dry, obtains white nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and water soluble mixt.
4., nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and water soluble mixt are pulverized, cross 200 mesh sieves and mix with auxiliary agent again.
In nucleic acid hydrolysis process described in this preparation method, alkali lye used is sodium hydroxide or potassium hydroxide solution.
In the present invention, nucleosides hydrochlorate mainly refers to: 5 '-adenylate disodium or potassium, 5 '-bird adenylate disodium or potassium, 5 '-cytidylic acid disodium or potassium, sodium salt or the sylvite of four kind 5 '-mixed nucleotides of 5 '-uridine monophosphate disodium or potassium; Deoxyribonucleoside hydrochlorate refers to: 5 '-desoxyadenossine acid disodium or potassium, 5 '-deoxidation bird adenylate disodium or potassium, 5 '-deoxycytidine acid disodium or potassium, 5 '-BrdU acid disodium,, sodium salt or the sylvite of five kind 5 '-deoxidation mixed nucleotides of 5 '-deoxyribosylthymine acid disodium or potassium.The source of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate is for take nucleic acid as raw material, be mainly mycelium from fermentation plant fermentation to adopt the nucleic acid of dry method or wet production be raw material, this kind of nucleic acid be take ribonucleic acid (RNA) as a small amount of deoxidation core of main mixing
Ribosomal ribonucleic acid (DNA), makes with alkali lye hydrolysis; Auxiliary agent is mainly derived from two aspects: 1, the nucleic acid of dry method or wet production also contains the impurity such as sugar, protein, various metals or nonmetallic ion, has (impurity) such as amino acid, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese after hydrolysis; 2, exogenous adding: sugar (reducing sugar, non reducing sugar: as glucose, sucrose also have fructose etc.), amino acid, big or middle, micro-etc.
The present invention adopt mainly by nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, the Promoting plant growth nutrient that auxiliary agent forms and for take nucleic acid as raw material, mainly from fermentation plant (Gourmet Powder Factory, brewery, antibiotics plant, yeast factory, Amino Acid Factory, paper mill, activated sludge after waste water treatment, petro-jelly fermentations etc.) nucleic acid of the employing dry method of by product (offcuts) mycelium after fermentation or wet production is the preparation method of raw material hydrolysis, with existing product and the method (product that adds Ribavirin, the product being directly hydrolyzed with ribonucleic acid, enzyme solution) compare and have the following advantages: 1, it is low that mycelium from fermentation plant fermentation is produced its production cost of ribonucleic acid, be easy to large-scale production and popularization, 2, the nucleic acid that mycelium is produced is containing useful impurity, in addition basic hydrolysis method, technique is simple, in mixed system, nucleotide salt yield is high, and various nucleosides hydrochlorates and the mutual mixing ratio of deoxynucleotide salt face are reasonable, and amino acid, sugar part and other organic-inorganic auxiliary agent content are higher, therefore, increasing both production and income synergy, strengthens resistance, improves quality effective, 3,, nontoxic, harmless, noresidue, without any adverse side effect, 4, be widely used in grain, cotton, oil, cigarette, tea, really, plant and the bacterium mushroom series products such as dish, traditional Chinese medicine, flowers, forest.
Embodiment
Embodiment 1: get 1 gram of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, and 6 grams of glucose, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient.
Embodiment 2: get 1 gram of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, and 2 grams of sucrose, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient.
Embodiment 3: get 2 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, 8 grams of glucose, nucleosides hydrochlorate and de-
Oxygen nucleosides hydrochlorate is pulverized, and mixes with glucose, obtains nutrient.
Embodiment 4: get 1.5 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, and 8.5 grams of glucose, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient.
Embodiment 5: get 1 gram of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, and 9 grams of glucose, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient.
Embodiment 6: get 2.5 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, and 7.5 grams of glucose, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient.
Embodiment 7: get 4 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, and 6 grams, fructose, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient.
Embodiment 8: get 5 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, and 5 grams of glucose, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient.
Embodiment 9: get 1 gram, nucleotide potassium and deoxynucleotide potassium, and 9 grams of glucose, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, obtain nutrient.
Embodiment 10: get 1.5 grams, nucleotide potassium and deoxynucleotide potassium, and 8.5 grams of glucose, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, obtain nutrient.
Embodiment 11: get 2 grams, nucleotide potassium and deoxynucleotide potassium, and 8 grams of glucose, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, obtain nutrient.
Embodiment 12: get 2.5 grams, nucleotide potassium and deoxynucleotide potassium, and 7.5 grams of glucose, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, obtain nutrient.
Embodiment 13: get 3 grams, nucleotide potassium and deoxynucleotide potassium, and 7 grams of glucose, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, obtain nutrient.
Embodiment 14: get 1.5 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, and 4.5 grams of glucose, 4 grams, amino acid, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix with glucose, obtain nutrient.
Embodiment 15: get 2 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, and 4 grams of glucose, 4 grams, amino acid, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix with glucose, obtain nutrient.
Embodiment 16: get 3 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, and 4 grams of glucose, 3 grams, amino acid, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix with glucose, obtain nutrient.
Embodiment 17: get 2 grams of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorates, and 8 grams, amino acid, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix, and obtain nutrient.
Embodiment 18: by 100 grams, the crude product ribonucleic acid (RNA) making from the mycelium of fermentation plant's by product by dry method or wet method, be dissolved in the sodium hydroxide solution of 3 liters of 6.3N, under 37 ℃ of conditions, stir 12 hours, winner will refer to and contain: 5 '-adenylate disodium, 5 '-bird adenylate disodium, 5 '-cytidylic acid disodium, the sodium salt of four kind 5 '-mixed nucleotides of 5 '-uridine monophosphate disodium also has deoxyribonucleoside hydrochlorate: 5 '-desoxyadenossine acid disodium, 5 '-deoxidation bird adenylate disodium, 5 '-deoxycytidine acid disodium, 5 '-BrdU acid disodium, , sodium salt and the impurity (amino acid of five kind 5 '-deoxidation mixed nucleotides of 5 '-deoxyribosylthymine acid disodium, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc.) solution, filter, filtrate is neutralized to pH8-8.5, be evaporated to appropriate, concentrate is added to activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 15%, by proper amount of clear water, dissolve nearly dry new-nucleo and deoxyribonucleotide natrium, its solution adds alcohol and separates out, crystallization suction filtration is dry, approximately obtain 85-90 grams, white nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and a certain amount of helping and agent water soluble mixt, gains are pulverized, crossed 200 mesh sieves, mix with auxiliary agent.
Embodiment 19: by 100 grams, the crude product ribonucleic acid (RNA) making from the mycelium of fermentation plant's by product by dry method or wet method, be dissolved in the potassium hydroxide solution of 2.5 liter of 6 N, under 40 ℃ of conditions, stir 10 hours, winner will refer to and contain: 5 '-adenylate dipotassium, 5 '-bird adenylate dipotassium, 5 '-cytidylic acid dipotassium, the sylvite of four kind 5 '-mixed nucleotides of 5 '-uridylic acid dipotassium also has deoxyribonucleoside hydrochlorate: 5 '-deoxyadenylic acid dipotassium, 5 '-deoxidation bird adenylate dipotassium, 5 '-deoxycytidylic acid dipotassium, 5 '-
Deoxyuridylic acid dipotassium,, the sylvite of five kind 5 '-deoxidation mixed nucleotides of 5 '-deoxythymidylic acid dipotassium and amino acid, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc.) and solution, filters, filtrate is neutralized to pH8-8.5, is evaporated to appropriate; Concentrate is added to activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into 20%; By proper amount of clear water, dissolve nearly dry nucleotide potassium and deoxynucleotide potassium, its solution adds alcohol separates out, and crystallization suction filtration is dry, approximately obtains 88-93 grams of white nucleotide sylvite and deoxyribonucleoside acid potassium salt and a certain amount of water soluble mixt (impurity).Gains are pulverized, crossed 200 mesh sieves, mix with auxiliary agent.
Embodiment 20: paddy rice: spray twice, for initial bloom stage (heading 10% left and right) is watered 30 liters with 20 grams of nutrients (containing glucose 80-85%'s), concentration 100ppm left and right, evenly sprays on one mu of paddy rice for the first time.Be full-bloom stage for the second time, within 5-6 days after spraying for the first time, with 20 grams of nutrients, be watered 30 liters, evenly spray on one mu of paddy rice and get final product (70-100 kilograms of mu volume increase, volume increase 15% left and right).
Embodiment 21: cotton: spray twice, for the first time also can initial bloom stage in the flower bud phase, and with 30 grams of nutrients, be watered 30 liters, evenly spray on one mu of cotton.Full-bloom stage is watered 36 liters with 30 grams evenly spray on one mu of cotton can (mu volume increase be more than 7%) for the second time.
Embodiment 22: rape: spraying twice, is initial bloom stage for the first time, be watered 20 liters spray on one mu of rape with 20 grams of nutrients.Be full-bloom stage for the second time, be watered 30 liters with 20 grams, spray on one mu of rape, volume increase 15-20 kilogram.
Embodiment 23: tobacco: spray twice, with 30 grams, be often watered 30 kilograms, concentration 200ppm left and right at every turn, spray on one mu of tobacco leaf, spray and carry out 20-26 day in time in May, (the south north time is slightly different), sprays for the first time, 3-6 day in June sprays for the second time, concentration 150ppm left and right, quality improves a grade, volume increase 20% left and right.
Embodiment 24: tealeaves: at growth germination period, spray for the first time, after every harvesting, spray once, concentration 180ppm left and right.
Embodiment 25: capsicum: initial bloom stage and full-bloom stage each once, concentration 200-300ppm, mu volume increase 40%～80%.
Embodiment 26: strawberry: in the fruitlet phase, spray, be watered 18 liters spray with 10 grams, spray 3～4 times in batches and can increase production 38% after harvesting.
Embodiment 27: traditional Chinese medicine: the traditional Chinese medicine of different parts, using method differs, generally initial bloom stage and full-bloom stage each once, concentration 100-260ppm.
Embodiment 28: mushroom: from fruiting, within every 3 days, spray once, and concentration 100-150ppm, volume increase 50-80%, shortens collecting time 1/3rd.
Claims (5)
1. the implantation methods of mushroom, is characterized in that: during mushroom growth, use nutrient; Described nutrient is mainly comprised of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, sugar or amino acid, its percentage by weight is: nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 10-80%, sugar or amino acid 20-90%, the time that described growing period is used nutrient is: from fruiting, within every 3 days, spray once, the concentration of using nutrient is 100-150ppm.
2. according to the implantation methods of mushroom described in claim 1, it is characterized in that: the nucleosides hydrochlorate in described nutrient and deoxyribonucleoside hydrochlorate and sugar or amino acid, its percentage by weight is: nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 10-30%, sugar or amino acid 70-90%.
3. according to the implantation methods of mushroom described in claim 1, it is characterized in that: nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are nucleotide potassium or sodium salt and deoxynucleotide potassium or sodium salt.
4. according to the implantation methods of mushroom described in claim 1, it is characterized in that: the sugar in described nutrient is glucose or sucrose or fructose.
5. according to the implantation methods of mushroom described in claim 1, it is characterized in that: in the nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate of described nutrient, percentage by weight is: nucleosides hydrochlorate 85-99%, deoxyribonucleoside hydrochlorate 1-15%.
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| CN1098437A (en) * | 1994-04-25 | 1995-02-08 | 于建国 | Humic acid nutrient liquor and preparation method thereof |
| CN1376654A (en) * | 2002-04-29 | 2002-10-30 | 孙永林 | Fertilizer dedicated for graminous plants and its application method |
| CN101186534A (en) * | 2007-12-03 | 2008-05-28 | 陕西省微生物研究所 | Method for preparing foliage fertilizer containing nucleic acid degradation product |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1098437A (en) * | 1994-04-25 | 1995-02-08 | 于建国 | Humic acid nutrient liquor and preparation method thereof |
| CN1376654A (en) * | 2002-04-29 | 2002-10-30 | 孙永林 | Fertilizer dedicated for graminous plants and its application method |
| CN101186534A (en) * | 2007-12-03 | 2008-05-28 | 陕西省微生物研究所 | Method for preparing foliage fertilizer containing nucleic acid degradation product |
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