CN102079665B - Nutrient for enhancing plant growth and preparation method thereof - Google Patents
Nutrient for enhancing plant growth and preparation method thereof Download PDFInfo
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- CN102079665B CN102079665B CN 201010514217 CN201010514217A CN102079665B CN 102079665 B CN102079665 B CN 102079665B CN 201010514217 CN201010514217 CN 201010514217 CN 201010514217 A CN201010514217 A CN 201010514217A CN 102079665 B CN102079665 B CN 102079665B
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- Prior art keywords
- hydrochlorate
- deoxynucleoside
- nucleosides
- sugar
- nutrient substance
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- 235000015097 nutrients Nutrition 0.000 title claims abstract description 48
- 230000008635 plant growth Effects 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 230000002708 enhancing effect Effects 0.000 title abstract description 4
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 40
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 40
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 37
- 150000001413 amino acids Chemical class 0.000 claims abstract description 30
- 238000000034 method Methods 0.000 claims abstract description 19
- 230000008569 process Effects 0.000 claims abstract description 3
- 239000002777 nucleoside Substances 0.000 claims description 60
- 125000003835 nucleoside group Chemical group 0.000 claims description 60
- 239000000126 substance Substances 0.000 claims description 43
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 35
- 239000008103 glucose Substances 0.000 claims description 35
- 239000000203 mixture Substances 0.000 claims description 25
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 21
- 230000001737 promoting effect Effects 0.000 claims description 17
- 239000002253 acid Substances 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 8
- -1 filter Substances 0.000 claims description 8
- 239000000706 filtrate Substances 0.000 claims description 8
- 239000002994 raw material Substances 0.000 claims description 8
- 238000000967 suction filtration Methods 0.000 claims description 8
- 230000007062 hydrolysis Effects 0.000 claims description 7
- 238000006460 hydrolysis reaction Methods 0.000 claims description 7
- 239000001103 potassium chloride Substances 0.000 claims description 7
- 239000003513 alkali Substances 0.000 claims description 6
- 238000002425 crystallisation Methods 0.000 claims description 6
- 230000008025 crystallization Effects 0.000 claims description 6
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 5
- 235000011164 potassium chloride Nutrition 0.000 claims description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
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- 238000005903 acid hydrolysis reaction Methods 0.000 claims description 2
- 239000002585 base Substances 0.000 claims description 2
- 150000007516 brønsted-lowry acids Chemical class 0.000 claims description 2
- 150000007528 brønsted-lowry bases Chemical class 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 239000012266 salt solution Substances 0.000 claims description 2
- 159000000000 sodium salts Chemical class 0.000 claims description 2
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims 1
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- 230000004151 fermentation Effects 0.000 abstract description 12
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- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 3
- 235000009024 Ceanothus sanguineus Nutrition 0.000 abstract 1
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- KCIDZIIHRGYJAE-YGFYJFDDSA-L dipotassium;[(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] phosphate Chemical compound [K+].[K+].OC[C@H]1O[C@H](OP([O-])([O-])=O)[C@H](O)[C@@H](O)[C@H]1O KCIDZIIHRGYJAE-YGFYJFDDSA-L 0.000 description 9
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- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 3
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 3
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 3
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 3
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 229910052791 calcium Inorganic materials 0.000 description 3
- 229910052802 copper Inorganic materials 0.000 description 3
- 239000010949 copper Substances 0.000 description 3
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- 239000012467 final product Substances 0.000 description 3
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- 239000011777 magnesium Substances 0.000 description 3
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- 229910052757 nitrogen Inorganic materials 0.000 description 3
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- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 2
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- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
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- IERHLVCPSMICTF-XVFCMESISA-N cytidine 5'-monophosphate Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(O)=O)O1 IERHLVCPSMICTF-XVFCMESISA-N 0.000 description 2
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- KURVIXMFFSNONZ-WFIJOQBCSA-L disodium;[(2r,3s,4r,5r)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound [Na+].[Na+].O[C@@H]1[C@H](O)[C@@H](COP([O-])([O-])=O)O[C@H]1N1C(=O)NC(=O)C=C1 KURVIXMFFSNONZ-WFIJOQBCSA-L 0.000 description 2
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- CKTSBUTUHBMZGZ-SHYZEUOFSA-N 2'‐deoxycytidine Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 CKTSBUTUHBMZGZ-SHYZEUOFSA-N 0.000 description 1
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Landscapes
- Saccharide Compounds (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fertilizers (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to a nutrient for enhancing plant growth and a preparation method thereof. The nutrient comprises the following components in percent by weight: 20-80 percent of nucleotide salts and deoxynucleotide salts and 20-80 percent of sugar or amino acid. The preparation method comprises the following steps of: preparing nucleic acid from mycelium in side products of a fermentation plant, next, hydrolyzing the nucleic acid into the nucleotide salts and the deoxynucleotide salts, and then mixing the nucleotide salts and the deoxynucleotide salts with the sugar or amino acid. The invention has the advantages that (1) the nutrient has no toxicity, harm, residue and any adverse side effect to plants; (2) the nutrient is beneficial to increase of the yield, the income and the efficiency, and has good effects of enhancing the resistance and improving the quality for plants; and (3) the preparation method has a simple process and low cost and is easy for large-scale production and promotion. The nutrient is widely applied to plants such as grain crops, cotton crops, oil crops, tobaccos, tea trees, fruit trees, vegetables, mushrooms, Chinese medicinal plants, flowers, forests and the like.
Description
Technical field
The present invention relates to a kind of nutrient substance of Promoting plant growth, particularly a kind of fertilizer that promotes plant growth and preparation method thereof.
Background technology
Greatly develop plant husbandry, forestry, promote that crop yield increases income, make our country green, it is the problem that the various countries scientist pays close attention to research, the growth of plant be unable to do without to supplement the nutrients, and present plant husbandry is mainly by executing N,P,K fertilizers and organic fertilizer promotes crop growth, use too much fertilizer very large on the quality and flavor impact of farm crop, be unfavorable for development and food safety and the environmental protection of green agriculture.Therefore, people have studied growth hormone relevant to nucleic acid, nutrient substance, fertilizer.In theory: nucleic acid is the basic substance that consists of life, is the basis of substance metabolism and energy metabolism, and the character of nucleic acid and the difference of quantity can cause the diversity of biological species and growth conditions.For congener life entity, the quantity of nucleic acid and the difference of existence form have determined strong and weak prosperous the declining of this life entity.Therefore, nucleic acid substances has irreplaceable effect for the output raising in farm crop and even the production of whole vegitabilia and product quality improvement; Particularly several nucleotides salt and deoxynucleoside hydrochlorate and the cooperations thereof of nucleic acid small molecules such as nucleosides, Nucleotide, pyrimidine and purine, the not only growth of regulating plant very effectively, can be used as again nutrition source is absorbed rapidly by plant, strengthen the plant root vigor, improve the plant anti-adversity ability, thus improve crop output, improve product quality.The nucleic acid small molecules has the enhancing improving activity of root system to plant, improves root to the absorption of phosphorus, potash fertilizer; Improve the activity of farm crop malic enzyme and succsinic acid enzyme, promote the assimilative capacity of plant, strengthen photosynthetic intensity, to plant-growth have regulate and trophism (Wang Xi etc. the application and function research on mechanism [J] of nucleic acid degradation product on Rice Production. Acta Biochimica et Biophysica Sinica, 1975,7 (1): 31-39).Chinese patent literature (publication number: 101186534) disclose a kind of preparation method who contains the nucleic acid degradation product foliage fertilizer, the concrete step is: 1., the lixiviate nuclease, 2., dissolving Yeast Nucleic Acid (alkali lye hydrotropy), 3., the hydrolysis of nucleic acid, must contain nucleosides, Nucleotide, purine, the multiple nucleic acids small molecules such as pyrimidine and a small amount of reducing sugar and few large, in, trace element, the method is take Yeast Nucleic Acid as raw material, with enzymatic hydrolysis, exist production cost high, degrade not thorough, seldom contain the deoxynucleotide composition, in mixed system, Nucleotide salt yield is low, the mutual mixing ratio imbalance of various nucleosides hydrochlorates and deoxynucleotide salt face, very large on the result of use impact.Chinese patent literature (publication number: 101050143) disclose a kind of nutrient substance that promotes crop growth, it is comprised of ribavirin and glucose, production and the guanosine of ribavirin are in close relations, and there is the ribavirin high cost in this patent, the unconspicuous problem of practical effect; Chinese patent literature (publication number: the preparation method who 1238323) discloses a kind of nucleic acid foliage fertilizer, the method of alkaline hydrolysis is adopted in the degraded of its nucleic acid, degradation product is purine and pyrimidine, there is the nucleic acid high cost equally in this patent, various nucleosides hydrochlorates and the mutual mixing ratio of deoxynucleotide salt face are unreasonable, affect the problem of result of use.
Summary of the invention
The purpose of this invention is to provide a kind of nutrient substance that promotes the growth of plant thing and preparation method thereof, its production cost is low, and technique is simple, is easy to large-scale production and popularization; In mixed system, Nucleotide salt yield is high, and various nucleosides hydrochlorates and the mutual mixing ratio of deoxynucleotide salt face are reasonable, nontoxic, harmless, noresidue, without any adverse side effect; Increase production, increase income, synergy, strengthen resistance, improve quality effective;
The present invention is achieved in that
The nutrient substance of Promoting plant growth, it mainly is comprised of nucleosides hydrochlorate and deoxynucleoside hydrochlorate, sugar or amino acid, and its weight percent is: nucleosides hydrochlorate and deoxynucleoside hydrochlorate 10-80%, sugar or amino acid 20-90%.
The nutrient substance of Promoting plant growth, in nucleosides hydrochlorate and deoxynucleoside hydrochlorate, weight percent is: nucleosides hydrochlorate 85-99%, deoxynucleoside hydrochlorate 1-15%.
The nutrient substance of Promoting plant growth, in nucleosides hydrochlorate and deoxynucleoside hydrochlorate, weight percent is: nucleosides hydrochlorate 88-96%, deoxynucleoside hydrochlorate 4-12%.
The nutrient substance of Promoting plant growth, nucleosides hydrochlorate and deoxynucleoside hydrochlorate and auxiliary agent, weight percent is: nucleosides hydrochlorate and deoxynucleoside hydrochlorate 10-30%, sugar or amino acid 70-90%.
The nutrient substance of Promoting plant growth, described sugar are glucose.
The nutrient substance of Promoting plant growth, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are sodium salt or sylvite.
The preparation method of the nutrient substance of Promoting plant growth provided by the present invention, concrete steps are as follows: take nucleic acid as raw material, be mainly that to adopt the nucleic acid of dry method or wet production be raw material for mycelium after the fermentation plant fermentation:
1., the hydrolysis of nucleic acid is the ratio mixed nucleus bronsted lowry acids and bases bronsted lowry liquid of 1:20-30 according to nucleic acid, buck weight ratio, alkali lye is 5.5-7N, under 30-45 ℃ of condition, stirred 10-14 hours, and got nucleosides hydrochlorate and deoxynucleoside acid salt solution, filter, filtrate is transferred pH8-8.5, concentrating under reduced pressure;
2., decolouring, dry concentrated solution is added activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 10-25%;
3., nucleosides hydrochlorate and the deoxynucleoside hydrochlorate of crystallization after doing with clear water dissolving, its solution adds alcohol separates out, and the crystallization suction filtration is dry, gets white nucleosides hydrochlorate and deoxynucleoside hydrochlorate and water soluble mixt;
4., nucleosides hydrochlorate and deoxynucleoside hydrochlorate and water soluble mixt are pulverized, cross 200 mesh sieves and mix evenly with sugar or amino acid again.
In the described nucleic acid hydrolysis process of this preparation method, alkali lye used is sodium hydroxide or potassium hydroxide solution.In the present invention, the nucleosides hydrochlorate mainly refers to: 5 '-adenylic acid (AMP) disodium or potassium, 5 '-bird adenylic acid (AMP) disodium or potassium, 5 '-cytidylic acid disodium or potassium, 5 '-four kind 5, uridine monophosphate disodium or potassium '-sodium salt or the sylvite of mixed nucleotides; The deoxynucleoside hydrochlorate refers to: 5 '-Desoxyadenosine acid disodium or potassium, 5 '-deoxidation bird adenylic acid (AMP) disodium or potassium, 5 '-Deoxyribose cytidine acid disodium or potassium, 5 '-the deoxyuridine acid disodium,, 5 '-five kind 5, deoxythymidine acid disodium or potassium '-sodium salt or the sylvite of deoxidation mixed nucleotides.The source of nucleosides hydrochlorate and deoxynucleoside hydrochlorate is take nucleic acid as raw material, mainly that to adopt the nucleic acid of dry method or wet production be raw material for mycelium after the fermentation plant fermentation, this kind nucleic acid is made with the alkali lye hydrolysis take Yeast Nucleic Acid (RNA) as a small amount of thymus nucleic acid of main mixing (DNA); Sugar or amino acid and other composition that comprises are mainly derived from two aspects: 1, the nucleic acid of dry method or wet production also contains the impurity such as sugar, protein, various metals or nonmetallic ion, and amino acid, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc. are arranged after hydrolysis; 2, exogenous adding: sugar (reducing sugar, non-reducing sugar: also have fructose etc. as glucose, sucrose), amino acid, big or middle, micro-etc.
the present invention adopt mainly by nucleosides hydrochlorate and deoxynucleoside hydrochlorate, it is take nucleic acid as raw material that the Promoting plant growth nutrient substance that sugar or amino acid form reaches, mainly from fermentation plant (Gourmet Powder Factory, brew-house, antibiotics plant, yeast factory, Amino Acid Factory, the paper mill, active sludge after sewage disposal, petroleum fermentations etc.) nucleic acid of the employing dry method of byproduct (tankage) mycelium after the fermentation or wet production is the preparation method of raw material hydrolysis, with currently available products and the method (product that adds ribavirin, the product that directly is hydrolyzed with Yeast Nucleic Acid, enzyme solution) compare and have the following advantages: 1, its production cost of mycelium production Yeast Nucleic Acid after the fermentation plant fermentation is low, be easy to large-scale production and popularization, 2, the nucleic acid of mycelium production contains useful impurity, basic hydrolysis method in addition, technique is simple, in mixed system, Nucleotide salt yield is high, and various nucleosides hydrochlorates and the mutual mixing ratio of deoxynucleotide salt face are reasonable, and amino acid, sugar part and other organic-inorganic component content are higher, therefore, increasing both production and income synergy strengthens resistance, improves quality effective, 3,, nontoxic, harmless, noresidue, without any adverse side effect, 4, be widely used in grain, cotton, oil, cigarette, tea, really, plant and the bacterium mushroom series products such as dish, Chinese medicinal materials, flowers, forest.
Embodiment
Embodiment 1: get nucleosides hydrochlorate and deoxynucleoside hydrochlorate 1 gram, and glucose 6 grams, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with glucose, namely get nutrient substance.
Embodiment 2: get nucleosides hydrochlorate and deoxynucleoside hydrochlorate 1 gram, and sucrose 2 grams, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with glucose, namely get nutrient substance.
Embodiment 3: get nucleosides hydrochlorate and deoxynucleoside hydrochlorate 2 grams, and glucose 8 grams, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with glucose, namely get nutrient substance.
Embodiment 4: get nucleosides hydrochlorate and deoxynucleoside hydrochlorate 1.5 grams, and glucose 8.5 grams, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with glucose, namely get nutrient substance.
Embodiment 5: get nucleosides hydrochlorate and deoxynucleoside hydrochlorate 1 gram, and glucose 9 grams, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with glucose, namely get nutrient substance.
Embodiment 6: get nucleosides hydrochlorate and deoxynucleoside hydrochlorate 2.5 grams, and glucose 7.5 grams, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with glucose, namely get nutrient substance.
Embodiment 7: get nucleosides hydrochlorate and deoxynucleoside hydrochlorate 4 grams, and fructose 6 grams, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with glucose, namely get nutrient substance.
Embodiment 8: get nucleosides hydrochlorate and deoxynucleoside hydrochlorate 5 grams, and glucose 5 grams, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with glucose, namely get nutrient substance.
Embodiment 9: get Nucleotide potassium and deoxynucleotide potassium 1 gram, and glucose 9 grams, Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, namely get nutrient substance.
Embodiment 10: get Nucleotide potassium and deoxynucleotide potassium 1.5 grams, and glucose 8.5 grams, Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, namely get nutrient substance.
Embodiment 11: get Nucleotide potassium and deoxynucleotide potassium 2 grams, and glucose 8 grams, Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, namely get nutrient substance.
Embodiment 12: get Nucleotide potassium and deoxynucleotide potassium 2.5 grams, and glucose 7.5 grams, Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, namely get nutrient substance.
Embodiment 13: get Nucleotide potassium and deoxynucleotide potassium 3 grams, and glucose 7 grams, Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, namely get nutrient substance.
Embodiment 14: get nucleosides hydrochlorate and deoxynucleoside hydrochlorate 1.5 grams, and glucose 4.5 grams, amino acid 4 grams, nucleosides hydrochlorate and deoxynucleoside hydrochlorate and amino acid are pulverized, and mix with glucose, namely get nutrient substance.
Embodiment 15: get nucleosides hydrochlorate and deoxynucleoside hydrochlorate 2 grams, and glucose 4 grams, amino acid 4 grams, nucleosides hydrochlorate and deoxynucleoside hydrochlorate and amino acid are pulverized, and mix with glucose, namely get nutrient substance.
Embodiment 16: get nucleosides hydrochlorate and deoxynucleoside hydrochlorate 3 grams, and glucose 4 grams, amino acid 3 grams, nucleosides hydrochlorate and deoxynucleoside hydrochlorate and amino acid are pulverized, and mix with glucose, namely get nutrient substance.
Embodiment 17: get nucleosides hydrochlorate and deoxynucleoside hydrochlorate 2 grams, and amino acid 8 grams, nucleosides hydrochlorate and deoxynucleoside hydrochlorate and amino acid are pulverized, and mix, and namely get nutrient substance.
embodiment 18: crude product Yeast Nucleic Acid (RNA) 100 grams that will make from the mycelium of fermentation plant's byproduct with dry method or wet method, be dissolved in the sodium hydroxide solution of 3 liters of 6.3N, under 37 ℃ of conditions, stirred 12 hours, the winner will refer to contain: 5 '-the adenylic acid (AMP) disodium, 5 '-bird adenylic acid (AMP) disodium, 5 '-the cytidylic acid disodium, 5 '-four kind 5 of uridine monophosphate disodium '-sodium salt of mixed nucleotides (also have the deoxynucleoside hydrochlorate: 5 '-the Desoxyadenosine acid disodium, 5 '-deoxidation bird adenylic acid (AMP) disodium, 5 '-the Deoxyribose cytidine acid disodium, 5 '-the deoxyuridine acid disodium, , 5 '-five kind 5 of deoxythymidine acid disodium '-sodium salt and the amino acid of deoxidation mixed nucleotides, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc.) solution, filter, filtrate is neutralized to pH8-8.5, be evaporated to appropriate, concentrated solution is added activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 15%, with nearly new-nucleo and the Sodium Deoxyribomuleotide of doing of proper amount of clear water dissolving, its solution adds alcohol and separates out, the crystallization suction filtration is dry, approximately get 85-90 grams, white nucleosides hydrochlorate and deoxynucleoside hydrochlorate and a certain amount of helping and the agent water soluble mixt, gains are pulverized, crossed 200 mesh sieves, mix with sugar or amino acid (when again adding sugar or amino acid).
embodiment 19: crude product Yeast Nucleic Acid (RNA) 100 grams that will make from the mycelium of fermentation plant's byproduct with dry method or wet method, be dissolved in the potassium hydroxide solution of 2.5 liter of 6 N, under 40 ℃ of conditions, stirred 10 hours, the winner will refer to contain: 5 '-the adenylic acid (AMP) dipotassium, 5 '-bird adenylic acid (AMP) dipotassium, 5 '-the cytidylic acid dipotassium, 5 '-four kind 5 of uridylic acid dipotassium '-sylvite of mixed nucleotides (also have the deoxynucleoside hydrochlorate: 5 '-the deoxyadenylic acid dipotassium, 5 '-deoxidation bird adenylic acid (AMP) dipotassium, 5 '-the deoxycytidylic acid(dCMP) dipotassium, 5 '-the deoxyuridylic acid dipotassium, , 5 '-five kind 5 of deoxythymidylic acid dipotassium '-sylvite and the amino acid of deoxidation mixed nucleotides, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc.) solution, filter, filtrate is neutralized to pH8-8.5, be evaporated to appropriate, concentrated solution is added activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into 20%, with nearly Nucleotide potassium and the deoxynucleotide potassium of doing of proper amount of clear water dissolving, its solution adds alcohol separates out, and the crystallization suction filtration is dry, approximately gets 88-93 gram white Nucleotide sylvite and deoxynucleoside acid potassium salt and a certain amount of auxiliary agent and water soluble mixts.Gains are pulverized, crossed 200 mesh sieves, mix with sugar or amino acid and other composition of comprising when other composition of comprising (again add sugar or amino acid and).
Using method of the present invention:
1, paddy rice: spray twice, for initial bloom stage (heading 10% left and right) is watered 30 liters with 20 gram nutrient substances (containing glucose 80-85%'s), about concentration 100ppm, evenly spray on one mu of paddy rice for the first time.Be full-bloom stage for the second time, be watered 30 liters with 20 gram nutrient substances in 5-6 days after spraying for the first time, evenly spray on one mu of paddy rice and get final product (15% left and right is increased production in 70-100 kilograms of mu volume increase).
2, cotton: spray twice, for the first time also can initial bloom stage in the flower bud phase, be watered 30 liters with 30 gram nutrient substances, evenly spray on one mu of cotton.Full-bloom stage is watered 36 liters with 30 grams and evenly sprays on one mu of cotton and get final product (the mu volume increase is more than 7%) for the second time.
3, rape: spraying twice, is initial bloom stage for the first time, is watered 20 liters with 20 gram nutrient substances and sprays on one mu of rape.Be full-bloom stage for the second time, be watered 30 liters with 20 grams, spray on one mu of rape and get final product, volume increase 15-20 kilogram.
4, tobacco: spray twice, use 30 grams at every turn, often be watered 30 kilograms, about concentration 200ppm, spray on one mu of tobacco leaf, spray and carry out 20-26 day in time in May, (the south north time is slightly different) sprays for the first time, 3-6 day in June sprays for the second time, concentration 150ppm left and right, quality improves a grade, increases production 20% left and right.
5, tealeaves: at the growth germination period, spray for the first time, spray once after every harvesting, about concentration 180ppm.
6, capsicum: initial bloom stage and full-bloom stage each once, concentration 200-300ppm, mu volume increase 40%～80%.
7, strawberry: spray in the fruitlet phase, be watered 18 liters with 10 grams and spray, spray 3～4 times after harvesting in batches and can increase production 38%.
8, Chinese medicinal materials: the Chinese medicinal materials of different sites, using method differs, generally initial bloom stage and full-bloom stage each once, concentration 100-260ppm.
9, mushroom: sprayed once in every 3 days from fruiting, concentration 100-150ppm, collecting time 1/3rd is shortened in volume increase 50-80%.
Claims (8)
1. the nutrient substance of a Promoting plant growth, it is characterized in that: it mainly is comprised of nucleosides hydrochlorate and deoxynucleoside hydrochlorate, sugar or amino acid, and its weight percent is: nucleosides hydrochlorate and deoxynucleoside hydrochlorate 10-80%, sugar or amino acid 20-90%.
2. a kind of nutrient substance of Promoting plant growth according to claim 1, it is characterized in that: in nucleosides hydrochlorate and deoxynucleoside hydrochlorate, weight percent is: nucleosides hydrochlorate 85-99%, deoxynucleoside hydrochlorate 1-15%.
3. a kind of nutrient substance of Promoting plant growth according to claim 2, it is characterized in that: in nucleosides hydrochlorate and deoxynucleoside hydrochlorate, weight percent is: nucleosides hydrochlorate 88-96%, deoxynucleoside hydrochlorate 4-12%.
4. a kind of nutrient substance of Promoting plant growth according to claim 1, it is characterized in that: nucleosides hydrochlorate and deoxynucleoside hydrochlorate and sugar or amino acid, weight percent is: nucleosides hydrochlorate and deoxynucleoside hydrochlorate 10-30%, sugar or amino acid 70-90%.
5. according to claim 1, the nutrient substance of 2,3 or 4 described a kind of Promoting plant growths, it is characterized in that: described sugar is glucose.
6. a kind of nutrient substance of Promoting plant growth according to claim 5, it is characterized in that: nucleosides hydrochlorate and deoxynucleoside hydrochlorate are sodium salt or sylvite.
7. the preparation method of the nutrient substance of the described Promoting plant growth of claim 1, its spy levy in: take nucleic acid as raw material:
1., the hydrolysis of nucleic acid is the ratio mixed nucleus bronsted lowry acids and bases bronsted lowry liquid of 1:20-30 according to nucleic acid, buck weight ratio, alkali lye is 5.5-7N, under 30-45 ℃ of condition, stirred 10-14 hours, and got nucleosides hydrochlorate and deoxynucleoside acid salt solution, filter, filtrate is transferred pH8-8.5, concentrating under reduced pressure;
2., decolouring, dry concentrated solution is added activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 10-25%;
3., nucleosides hydrochlorate and the deoxynucleoside hydrochlorate of crystallization after doing with clear water dissolving, its solution adds alcohol separates out, and the crystallization suction filtration is dry, gets white nucleosides hydrochlorate and deoxynucleoside hydrochlorate and water soluble mixt;
4., nucleosides hydrochlorate and deoxynucleoside hydrochlorate and water soluble mixt are pulverized, cross 200 mesh sieves and mix evenly with sugar or amino acid again.
8. preparation method according to claim 7, is characterized in that, in described nucleic acid hydrolysis process, alkali lye used is sodium hydroxide or potassium hydroxide solution.
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| CN201310249570.XA Division CN103348809B (en) | 2010-10-21 | 2010-10-21 | Planting method for rice |
| CN201310249563.XA Division CN103348845B (en) | 2010-10-21 | 2010-10-21 | Method for planting good-quality and high-yield oilseed rape |
| CN201310249562.5A Division CN103351192B (en) | 2010-10-21 | 2010-10-21 | Planting method for tobaccos |
| CN201310249567.8A Division CN103351193B (en) | 2010-10-21 | 2010-10-21 | Good-quality and high-yield planting method for cotton |
| CN201310249547.0A Division CN103348867B (en) | 2010-10-21 | 2010-10-21 | Planting method of mushrooms |
| CN201310249548.5A Division CN103348842B (en) | 2010-10-21 | 2010-10-21 | Method for planting Chinese herbal medicine |
| CN201310249440.6A Division CN103351190B (en) | 2010-10-21 | 2010-10-21 | Application method of nutrient for enhancing plant growth |
| CN201310249560.6A Division CN103348843B (en) | 2010-10-21 | 2010-10-21 | Planting method of strawberries |
| CN201310249561.0A Division CN103348844B (en) | 2010-10-21 | 2010-10-21 | Planting method of chilies with good quality and high yield |
| CN201310249545.1A Division CN103351191B (en) | 2010-10-21 | 2010-10-21 | Planting method for tea leaves |
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- 2010-10-21 CN CN201310252497.1A patent/CN103351221B/en not_active Expired - Fee Related
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1098437A (en) * | 1994-04-25 | 1995-02-08 | 于建国 | Humic acid nutrient liquor and preparation method thereof |
| CN1376654A (en) * | 2002-04-29 | 2002-10-30 | 孙永林 | Fertilizer dedicated for graminous plants and its application method |
| CN101186534A (en) * | 2007-12-03 | 2008-05-28 | 陕西省微生物研究所 | Method for preparing foliage fertilizer containing nucleic acid degradation product |
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| Publication number | Publication date |
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| CN103351221B (en) | 2016-03-09 |
| CN102079665A (en) | 2011-06-01 |
| CN103351221A (en) | 2013-10-16 |
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