CN103613412A - Preparation method of composition containing nucleotide salt and deoxyribonucleotide salt - Google Patents
Preparation method of composition containing nucleotide salt and deoxyribonucleotide salt Download PDFInfo
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Abstract
The invention discloses a preparation method of a composition containing a nucleotide salt and a deoxyribonucleotide salt. The composition does not contain sugar or/and amino acid. The preparation method comprises the following steps: preparing a nucleic acid from a by-product mycelium of a fermentation factory; hydrolyzing with nucleic acid to prepare the nucleotide salt and the deoxyribonucleotide salt, or directly taking various products, namely the nucleotide salt and the deoxyribonucleotide salt, and then crushing, screening and evenly mixing. The composition has the purposes that the yield, the quality and the character can be improved when the composition is applied to production of traditional Chinese medicines, grains, oil plants, vegetables, fruits, tobacco leaves, cotton, tea, flowers and forests, the resistance and the uniformity are enhanced, the content of a noxious substance is reduced, the cost is saved, and the income and the efficiency are increased. The composition can be applied to promotion of plant growth as a fertilizer or a nutrient, and also can be applied to promotion of plant growth as a plant growth regulator. The preparation method has the advantages of low cost and wide use, and is simple and convenient to manufacture and use and is easy to produce and popularize on large scale.
Description
Technical field
The present invention relates to a kind of preparation method of composition who contains nucleosides hydrochlorate and deoxynucleoside hydrochlorate and purposes of said composition for Promoting plant growth.
Background technology
The growth of plant be unable to do without and supplements the nutrients, present plant husbandry, main by executing N,P,K fertilizers and organic fertilizer promotes crop growth, using too much fertilizer affects very greatly on the quality and flavor of farm crop, be unfavorable for development and food safety and the environmental protection of green agriculture.Therefore, people have studied growth hormone relevant to nucleic acid, nutrient substance, fertilizer.In theory: nucleic acid is the basic substance that forms life, is the basis of substance metabolism and energy metabolism, and the character of nucleic acid and the difference of quantity can cause the diversity of biological species and growth conditions.For congener life entity, the quantity of nucleic acid and the difference of existence form have determined prosperous the declining of power of this life entity.Therefore, nucleic acid substances has irreplaceable effect for the output raising in farm crop and even the production of whole vegitabilia and product quality improvement; Particularly several nucleotides salt and deoxynucleoside hydrochlorate and the cooperations thereof of nucleic acid small molecules such as nucleosides, Nucleotide, pyrimidine and purine, the not only growth of regulating plant very effectively, can be used as again nutrition source is absorbed rapidly by plant, strengthen plant root vigor, improve plant anti-adversity ability, thus improve crop output, improve product quality.Nucleic acid small molecules has enhancing improving activity of root system to plant, improves the absorption of root to phosphorus, potash fertilizer; Improve the activity of farm crop malic enzyme and succsinic acid enzyme, promote the assimilative capacity of plant, strengthen photosynthetic intensity, to plant-growth have regulate and trophism (Wang Xi etc. the research [J] of the application and function mechanism of nucleic acid degradation product in Rice Production. Acta Biochimica et Biophysica Sinica, 1975,7 (1): 31-39).Chinese patent literature (publication number: 101186534) disclose a kind of preparation method who contains nucleic acid degradation product foliage fertilizer, concrete steps are: 1., lixiviate nuclease, 2., dissolve Yeast Nucleic Acid (alkali lye hydrotropy), 3., the hydrolysis of nucleic acid, must contain nucleosides, Nucleotide, purine, the multiple nucleic acids small molecules such as pyrimidine and a small amount of reducing sugar and few large, in, trace element, the method be take Yeast Nucleic Acid as raw material, with enzymatic hydrolysis, exist production cost high, degrade not thorough, few containing deoxynucleotide composition, in mixed system, Nucleotide salt yield is low, the mutual mixing ratio imbalance of various nucleosides hydrochlorates and deoxynucleotide salt face, very large on result of use impact.The patent No.: 201010514217.6, Granted publication CN102079665B, name is called: the Chinese patent of " nutrient substance of a kind of Promoting plant growth and preparation method thereof ", a kind of nutrient substance of Promoting plant growth is disclosed, it is mainly comprised of this nutrient substance nucleosides hydrochlorate, deoxynucleoside hydrochlorate and sugar or amino acid, its weight percent is: nucleosides hydrochlorate and deoxynucleoside hydrochlorate 20-80%, sugar or amino acid 20-80%.This patent exists that composition activeconstituents is more does not save, is difficult for standing storage and preparation method is simple not, and purposes is problem not widely.
Summary of the invention
Object of the present invention overcomes above-mentioned deficiency exactly, a kind of activeconstituents in composition that reduced is provided, and its effect still can meet or exceed the patent No. and be: the preparation method of the composition that contains nucleosides hydrochlorate and deoxynucleoside hydrochlorate of " 201010514217.6 " nutrient substance.
The present invention is achieved in that
The nutrient substance of Promoting plant growth, is mainly comprised of nucleosides hydrochlorate and deoxynucleoside hydrochlorate, not sugary or/and amino acid.
The nutrient substance of Promoting plant growth, is characterized in that: in nucleosides hydrochlorate and deoxynucleoside hydrochlorate, weight percent is: nucleosides hydrochlorate 85-99%, deoxynucleoside hydrochlorate 1-15%.
The nutrient substance of Promoting plant growth, in nucleosides hydrochlorate and deoxynucleoside hydrochlorate, weight percent is: nucleosides hydrochlorate 88-96%, deoxynucleoside hydrochlorate 4-12%.
The nutrient substance of Promoting plant growth, it also comprises weighting agent: one or more of starch, dextrin, Mierocrystalline cellulose, polyvidone, Magnesium Stearate, calcium sulfate or magnesium sulfate.
The nutrient substance of Promoting plant growth, the weight percent of nucleosides hydrochlorate and deoxynucleoside hydrochlorate and weighting agent is: nucleosides hydrochlorate and deoxynucleoside hydrochlorate 10-80%, weighting agent 20-90%.
The nutrient substance of Promoting plant growth, is characterized in that: the weight percent of nucleosides hydrochlorate and deoxynucleoside hydrochlorate and weighting agent is: nucleosides hydrochlorate and deoxynucleoside hydrochlorate 10-30%, weighting agent 70-90%.
The nutrient substance of Promoting plant growth, weighting agent is: starch is or/and Mierocrystalline cellulose.
The nutrient substance of Promoting plant growth, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are sodium salt or sylvite.
The nutrient substance that promotes thing growth, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are sodium salt or sylvite.
The nutrient substance of Promoting plant growth, weighting agent is: one or more of W-Gum, cyclodextrin, Xylo-Mucine.
The nutrient substance of a kind of Promoting plant growth of the present invention also can be described as the composition that contains nucleosides hydrochlorate and deoxynucleoside hydrochlorate:
The preparation method of the composition that contains nucleosides hydrochlorate and deoxynucleoside hydrochlorate, composition is mainly comprised of nucleosides hydrochlorate and deoxynucleoside hydrochlorate, not sugary or/and amino acid, its preparation, take nucleic acid as raw material:
1., the hydrolysis of nucleic acid: the ratio mixed nucleus bronsted lowry acids and bases bronsted lowry liquid that is 1:20-30 according to nucleic acid, buck weight ratio, alkali lye is 5.5-7N, under 30-45 ℃ of condition, stir 10-14 hours, obtain nucleosides hydrochlorate and deoxynucleoside acid salt solution, filter, filtrate is adjusted pH8-8.5, concentrating under reduced pressure;
2., decolouring, dry concentrated solution is added to activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 10-25%;
3., crystallization dissolves nucleosides hydrochlorate and the deoxynucleoside hydrochlorate after dry with clear water, its solution adds alcohol separates out, and crystallization suction filtration is dry, obtains white nucleosides hydrochlorate and deoxynucleoside hydrochlorate;
4., nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, sieve.
Described preparation method, in described nucleic acid hydrolysis process, alkali lye used is sodium hydroxide or potassium hydroxide solution.
Described preparation method, it is characterized in that, composition also comprises weighting agent: one or more of starch, dextrin, Mierocrystalline cellulose, polyvidone, Magnesium Stearate, calcium sulfate or magnesium sulfate, add the weighting agent of pulverizing to mix with nucleosides hydrochlorate and deoxynucleoside hydrochlorate, the weight percent of nucleosides hydrochlorate and deoxynucleoside hydrochlorate and weighting agent is: nucleosides hydrochlorate and deoxynucleoside hydrochlorate 10-80%, weighting agent 20-90%.
The preparation method of the composition that contains nucleosides hydrochlorate and deoxynucleoside hydrochlorate, composition is mainly comprised of nucleosides hydrochlorate and deoxynucleoside hydrochlorate, not sugary or/and amino acid, its preparation: be by weight percentage: nucleosides hydrochlorate 85-99%, deoxynucleoside hydrochlorate 1-15%, get commercially available nucleosides hydrochlorate and deoxynucleoside hydrochlorate, mix, pulverize, sieve.
Described preparation method, composition also comprises weighting agent: one or more of starch, dextrin, Mierocrystalline cellulose, polyvidone, Magnesium Stearate, calcium sulfate or magnesium sulfate, add the weighting agent of pulverizing to mix with nucleosides hydrochlorate and deoxynucleoside hydrochlorate, the weight percent of nucleosides hydrochlorate and deoxynucleoside hydrochlorate and weighting agent is: nucleosides hydrochlorate and deoxynucleoside hydrochlorate 10-80%, weighting agent 20-90%.
The purposes of the composition that contains nucleosides hydrochlorate and deoxynucleoside hydrochlorate, said composition is mainly comprised of nucleosides hydrochlorate and deoxynucleoside hydrochlorate, not sugary or/and amino acid, weight percent is: nucleosides hydrochlorate 85-99%, deoxynucleoside hydrochlorate 1-15%, said composition is applied to Chinese medicine, grain, oil plant, vegetables, fruit, tobacco leaf, cotton, tealeaves, flowers, forest and produces and can improve its Yield and quality, quality, enhancing resistance, uniformity coefficient, reduce nuisance content, cost-saving, increase income, synergy.
The purposes of the composition that contains nucleosides hydrochlorate and deoxynucleoside hydrochlorate, said composition is mainly comprised of nucleosides hydrochlorate and deoxynucleoside hydrochlorate, not sugary or/and amino acid, the application on plant of said composition as fertilizer sources or nutrient substance.
The purposes of the composition that contains nucleosides hydrochlorate and deoxynucleoside hydrochlorate, its spy levy in: in composition, also contain weighting agent: one or more of starch, dextrin, Mierocrystalline cellulose, polyvidone, Magnesium Stearate, calcium sulfate or magnesium sulfate, this composition that contains weighting agent also can as fertilizer sources or nutrient substance on plant, apply.
The purposes of the composition that contains nucleosides hydrochlorate and deoxynucleoside hydrochlorate, said composition is mainly comprised of nucleosides hydrochlorate and deoxynucleoside hydrochlorate, not sugary or/and amino acid, said composition can be applied as plant-growth regulator on plant.
The purposes of the composition that contains nucleosides hydrochlorate and deoxynucleoside hydrochlorate, in composition, also contain weighting agent: one or more of starch, dextrin, Mierocrystalline cellulose, polyvidone, Magnesium Stearate, calcium sulfate or magnesium sulfate, this composition that contains weighting agent also can be applied as plant-growth regulator on plant.
Weighting agent of the present invention: starch refers to: green starch, yam starch, wheat starch, sweet potato starch, W-Gum, water caltrop starch, Lotus Root Starch, Chinese Water Chestnut Starch etc., preferably W-Gum.Dextrin refers to: white dextrin, yellow starch gum and Britain glue, cyclodextrin.Mierocrystalline cellulose refers to: methylcellulose gum, Vltra tears, Natvosol, carboxymethyl cellulose etc.
The preparation method of the nutrient substance of Promoting plant growth provided by the present invention, concrete steps are as follows: take nucleic acid as raw material: the one, take commercially available nucleic acid as raw material, 1., the hydrolysis of the nucleic acid ratio mixed nucleus bronsted lowry acids and bases bronsted lowry liquid that is 1:20-30 according to nucleic acid, buck weight ratio the 2nd, it is raw material that the mycelium from fermentation plant fermentation adopts the nucleic acid of dry method or wet production:, alkali lye is 5.5-7N, under 30-45 ℃ of condition, stir 10-14 hours, obtain nucleosides hydrochlorate and deoxynucleoside acid salt solution, filter, filtrate is adjusted pH8-8.5, concentrating under reduced pressure;
2., decolouring, dry concentrated solution is added to activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 10-25%;
3., crystallization is dissolved nucleosides hydrochlorate and the deoxynucleoside hydrochlorate after doing with clear water, its solution adds alcohol and separates out, crystallization suction filtration is dry, obtain white nucleosides hydrochlorate and deoxynucleoside hydrochlorate (it is impurity that the mycelium from fermentation plant fermentation adopts the water soluble mixt that also contains that the nucleic acid of dry method or wet production is raw material);
4., nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, cross 50-150 mesh sieve, mix.
In nucleic acid hydrolysis process described in this preparation method, alkali lye used is sodium hydroxide or potassium hydroxide solution.
Commercially available nucleic acid is seldom impure after raw material hydrolysis.
In the present invention, nucleosides hydrochlorate mainly refers to: 5 '-adenylic acid (AMP) disodium or potassium, 5 '-Sodium guanylate or potassium, 5 '-cytidylic acid disodium or potassium, sodium salt or the sylvite of four kind 5 '-mixed nucleotides of 5 '-uridine monophosphate disodium or potassium; Deoxynucleoside hydrochlorate refers to: 5 '-Desoxyadenosine acid disodium or potassium, 5 '-deoxidation bird adenylic acid (AMP) disodium or potassium, 5 '-Deoxyribose cytidine acid disodium or potassium, 5 '-deoxyuridine acid disodium,, sodium salt or the sylvite of five kind 5 '-deoxidation mixed nucleotides of 5 '-deoxythymidine acid disodium or potassium.The source of nucleosides hydrochlorate and deoxynucleoside hydrochlorate is for take nucleic acid as raw material, it is raw material that mycelium after the fermentation plant of take fermentation adopts the nucleic acid of dry method or wet production, this kind of nucleic acid be take Yeast Nucleic Acid (RNA) as a small amount of thymus nucleic acid of main mixing (DNA), with alkali lye hydrolysis, makes nucleosides hydrochlorate and deoxynucleotide salt mixture.
Beneficial effect of the present invention:
Because the present invention is not sugary or/and amino acid has reduced composition activeconstituents, so more economize in raw materials and cost.
Although the present invention has reduced activeconstituents, its effect does not still subtract and even produces better effect.
The present invention is not particularly containing glucose, glucose easily absorbs moisture deliquescence, the nutrient substance that contains glucose is placed the deliquescence that absorbs water more than a year under general condition of storage, there is product caking, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are further hydrolyzed, cause quality of products even to lose efficacy, so, the invention solves the problem that is difficult for standing storage of nutrient substance.
. because the present invention also comprises weighting agent: one or more of starch, dextrin, Mierocrystalline cellulose, polyvidone, Magnesium Stearate, calcium sulfate or magnesium sulfate etc., the adding to have adapted to of weighting agent are generally not less than the relevant regulations of 20 grams as the foliage fertilizer minimum package of merchandise sales; Filler starch, dextrin, Mierocrystalline cellulose etc. also possess certain viscosity, after spraying, make nutrient substance be attached to blade face and the plant surface time longer, make its action effect better; Weighting agent polyvidone, Magnesium Stearate, calcium sulfate or magnesium sulfate etc. have dispersiveness and oilness, can extend the quality guaranteed period of nutrient substance.
Due to preparation method of the present invention: 1, can byproduct (tankage) mycelium after fermentation plant (active sludge Gourmet Powder Factory, brew-house, antibiotics plant, yeast factory, Amino Acid Factory, paper mill, sewage disposal, petroleum fermentation etc.) fermentation adopting the nucleic acid of dry method or wet production is raw material, its price of this nucleic acid is low, is easy to cost-saving; 2, can adopt and take commercially available nucleic acid and be the preparation method of raw material hydrolysis, product high purity, the proportioning of Yeast Nucleic Acid and thymus nucleic acid can be adjusted, product suitability and better effects if; 3, also can be directly with commercially available 5 '-adenylic acid (AMP) disodium or potassium, 5 '-Sodium guanylate or potassium, 5 '-cytidylic acid disodium or potassium, 5 '-uridine monophosphate disodium or potassium and 5 '-Desoxyadenosine acid disodium or potassium, 5 '-deoxidation bird adenylic acid (AMP) disodium or potassium, 5 '-Deoxyribose cytidine acid disodium or potassium, 5 '-deoxyuridine acid disodium, the sodium salt of five kind 5 '-deoxidation mixed nucleotides of 5 '-deoxythymidine acid disodium or potassium or sylvite preparation, therefore applicable more flexible, technique is simpler.
Purposes of the present invention is: the particularly application on plant as foliage fertilizer or nutrient substance of as fertilizer sources; Also can on plant, apply as plant-growth regulator;
The present invention is applied to Chinese medicine, grain, oil plant, vegetables, fruit, tobacco leaf, cotton, tealeaves, flowers, forest and produces and can improve its Yield and quality, quality, enhancing resistance, uniformity coefficient, reduce nuisance content, cost-saving, increase income, synergy.
So product of the present invention: saving, environmental protection (nontoxic, harmless, noresidue, without any adverse side effect), volume increase of many uses, increase income, synergy, strengthen resistance, improve quality effective.
The present invention and No. 201010514217.6 patents, traditional product and method contrast tables 1
The present invention and No. 201010514217.6 patents, traditional product and method contrast tables 2
The present invention implements to spray agency part effect in Table 3 to concrete crop
Note: content's index detection method is carried out with reference to national standard or industry standard, content is pressed dry product and is calculated.
Embodiment
Embodiment 1: get 2 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and obtain nutrient substance.
Embodiment 2: get 4 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, nutrient substance is pulverized and obtained to nucleosides hydrochlorate and deoxynucleoside hydrochlorate.
Embodiment 3: get 6 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and obtain nutrient substance.
Embodiment 4: get 2 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, and 18 grams of starch, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with starch, obtain nutrient substance.
Embodiment 5: get 3 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, and 22 grams of W-Gums, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with W-Gum, obtain nutrient substance.
Embodiment 6: get 4 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, and 16 grams of wheat starches, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with wheat starch, obtain nutrient substance.
Embodiment 7: get 5 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, and 20 grams of white dextrins, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with white dextrin, obtain nutrient substance.
Embodiment 8: get 6 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, and 20 grams of cyclodextrin, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with cyclodextrin, obtain nutrient substance.
Embodiment 9: get 1 gram, Nucleotide potassium and deoxynucleotide potassium, and 9 grams of W-Gums, Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with W-Gum, obtain nutrient substance.
Embodiment 10: get 1.5 grams, Nucleotide potassium and deoxynucleotide potassium, and 8.5 grams of methylcellulose gum, Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with methylcellulose gum, obtain nutrient substance.
Embodiment 11: get 2 grams, Nucleotide potassium and deoxynucleotide potassium, and 8 grams of carboxymethyl celluloses etc., Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with carboxymethyl cellulose etc., obtain nutrient substance.
Embodiment 12: get 2.5 grams, Nucleotide potassium and deoxynucleotide potassium, and 7.5 grams of polyvidones, Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with polyvidone, obtain nutrient substance.
Embodiment 13: get 3 grams, Nucleotide potassium and deoxynucleotide potassium, and 7 grams of Magnesium Stearates, Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with Magnesium Stearate, obtain nutrient substance.
Embodiment 14: get 1.5 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, and 4.5 grams of W-Gums, 4 grams, magnesium sulfate, nucleosides hydrochlorate and deoxynucleoside hydrochlorate and magnesium sulfate are pulverized, and mix with W-Gum, obtain nutrient substance.
Embodiment 15: get 2 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, and 4 grams of Natvosols, 4 grams, calcium sulfate, nucleosides hydrochlorate and deoxynucleoside hydrochlorate and calcium sulfate are pulverized, and mix with Natvosol, obtain nutrient substance.
Embodiment 16: get 3 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, and 4 grams of W-Gums, 3 grams of Magnesium Stearates, nucleosides hydrochlorate and deoxynucleoside hydrochlorate and Magnesium Stearate are pulverized, and mix with W-Gum, obtain nutrient substance.
Execute example 17: get 7 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, 4 grams of W-Gums, 8 grams of yellow starch gums, 4 grams, calcium sulfate, nucleosides hydrochlorate and deoxynucleoside hydrochlorate and calcium sulfate are pulverized, and mix with W-Gum, yellow starch gum, obtain nutrient substance.
Embodiment 18: by 100 grams, the crude product Yeast Nucleic Acid (RNA) making from the mycelium of fermentation plant's byproduct by dry method or wet method, be dissolved in the sodium hydroxide solution of 3 liters of 6.3N, under 37 ℃ of conditions, stir 12 hours, winner will refer to and contain: 5 '-adenylic acid (AMP) disodium, 5 '-bird adenylic acid (AMP) disodium, 5 '-cytidylic acid disodium, the sodium salt of four kind 5 '-mixed nucleotides of 5 '-uridine monophosphate disodium (also has deoxynucleoside hydrochlorate: 5 '-Desoxyadenosine acid disodium, 5 '-deoxidation bird adenylic acid (AMP) disodium, 5 '-Deoxyribose cytidine acid disodium, 5 '-deoxyuridine acid disodium, , five kind 5 of the 5 '-deoxythymidine acid disodium '-sodium salt of deoxidation mixed nucleotides and solution of impurity), filter, filtrate is neutralized to pH8-8.5, be evaporated to appropriate, concentrated solution is added to activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 15%, by proper amount of clear water, dissolve nearly dry new-nucleo and Sodium Deoxyribomuleotide, its solution adds alcohol separates out, and crystallization suction filtration is dry, approximately obtains 85-90 grams, and white nucleosides hydrochlorate and deoxynucleoside hydrochlorate and water soluble mixt impurity are pulverized gains, cross 100 mesh sieves.
Embodiment 19: by 100 grams, the crude product Yeast Nucleic Acid (RNA) making from the mycelium of fermentation plant's byproduct by dry method or wet method, be dissolved in the potassium hydroxide solution of 2.5 liters of 6N, under 40 ℃ of conditions, stir 10 hours, winner will refer to and contain: 5 '-adenylic acid (AMP) dipotassium, 5 '-bird adenylic acid (AMP) dipotassium, 5 '-cytidylic acid dipotassium, the sylvite of four kind 5 '-mixed nucleotides of 5 '-uridylic acid dipotassium, also has deoxynucleoside hydrochlorate: 5 '-deoxyadenylic acid dipotassium, 5 '-deoxidation bird adenylic acid (AMP) dipotassium, 5 '-deoxycytidylic acid(dCMP) dipotassium, 5 '-deoxyuridylic acid dipotassium, , sylvite and the impurity (amino acid of five kind 5 '-deoxidation mixed nucleotides of 5 '-deoxythymidylic acid dipotassium, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc.) solution, filter, filtrate is neutralized to pH8-8.5, be evaporated to appropriate, concentrated solution is added to activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into 20%, by proper amount of clear water, dissolve nearly dry Nucleotide potassium and deoxynucleotide potassium, its solution adds alcohol separates out, and crystallization suction filtration is dry, approximately obtains 88-93 grams of white Nucleotide sylvite and deoxynucleoside acid potassium salt and water soluble mixt impurity.Gains are pulverized, crossed 80 mesh sieves.
Embodiment 20: by 90 grams of commercially available nucleosides hydrochlorates, 10 grams of deoxynucleoside hydrochlorates, mix, be dissolved in the potassium hydroxide solution of 2.5 liters of 6N, under 40 ℃ of conditions, stir 10 hours, must contain: 5 '-adenylic acid (AMP) dipotassium, 5 '-bird adenylic acid (AMP) dipotassium, 5 '-cytidylic acid dipotassium, the sylvite of four kind 5 '-mixed nucleotides of 5 '-uridylic acid dipotassium, also has deoxynucleoside hydrochlorate: 5 '-deoxyadenylic acid dipotassium, 5 '-deoxidation bird adenylic acid (AMP) dipotassium, 5 '-deoxycytidylic acid(dCMP) dipotassium, 5 '-deoxyuridylic acid dipotassium, , the solution of the sylvite of five kind 5 '-deoxidation mixed nucleotides of 5 '-deoxythymidylic acid dipotassium, filter, filtrate is neutralized to pH8-8.5, be evaporated to appropriate, concentrated solution is added to activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into 20%, by proper amount of clear water, dissolve nearly dry Nucleotide potassium and deoxynucleotide potassium, its solution adds alcohol separates out, and crystallization suction filtration is dry, approximately obtains 95-98 grams of white Nucleotide sylvite and deoxynucleoside acid potassium salt.Gains are pulverized, crossed 120 mesh sieves.
Embodiment 21; Paddy rice: spray twice, be watered 30 liters for the first time for initial bloom stage (10% left and right of earing) with 20 grams of nutrient substances, concentration 100ppm left and right, evenly sprays on one mu of paddy rice.Be full-bloom stage for the second time, within 5-6 days after spraying for the first time, with 20 grams of nutrient substances, be watered 30 liters, evenly spray on one mu of paddy rice and get final product (77-130 kilograms of mu volume increase, increase production 18% left and right).
Embodiment 22: spray twice, for the first time also can initial bloom stage in the flower bud phase, and with 30 grams of nutrient substances, be watered 30 liters, evenly spray on one mu of cotton.Full-bloom stage is watered 36 liters with 30 grams evenly spray on one mu of cotton can (mu volume increase be more than 9%) for the second time.
Embodiment 23: rape: spraying twice, is initial bloom stage for the first time, be watered 20 liters spray on one mu of rape with 20 grams of nutrient substances.Be full-bloom stage for the second time, be watered 30 liters with 20 grams, spray on one mu of rape, volume increase 20-25 kilogram.
Embodiment 24: tobacco: spray twice, with 30 grams, be often watered 30 kilograms, concentration 200ppm left and right at every turn, spray on one mu of tobacco leaf, spray and carry out 20-26 day in time in May, the south north time is slightly different), spray for the first time, 3-6 day in June sprays for the second time, concentration 150ppm left and right, quality improves a grade, increases production 24% left and right.
Embodiment 25: tealeaves: at growth germination period, spray for the first time, after every harvesting, spray once, concentration 180ppm left and right.Increase production 2% left and right.
Embodiment 26: capsicum: initial bloom stage and full-bloom stage each once, concentration 200-300ppm, mu volume increase 43%～85%.
Embodiment 27: strawberry: in the fruitlet phase, spray, be watered 18 liters spray with 10 grams, spray 3～4 times in batches and can increase production 44% after harvesting.
Embodiment 28: Chinese medicinal materials: the Chinese medicinal materials of different sites, using method differs, generally initial bloom stage and full-bloom stage each once, concentration 100-260ppm.
Embodiment 29: mushroom: from fruiting, within every 3 days, spray once, concentration 100-150ppm, increases production 55-87%, shorten collecting time 1/3rd.
Embodiment 30; Tree Peony flower: start to spray once for every 5 days from the petal phase of 10-20%, concentration 200-350ppm, the florescence can extend more than 7 days, and flower is many and large.
Embodiment 31: Root of Manyflower Glorybower: before results, February, sky sprayed once, and every bag is watered 18 kilograms, spray 1 mu of ground Root of Manyflower Glorybower.Volume increase 30%～40%.
Embodiment 32: the red sage root: spray twice, use 6 grams at every turn, every 3 grams are watered 18 kilograms, spray on one mu of red sage root, and the time that sprays sprays for the first time between June, spray for the second time 120 kilograms of mu volume increase between July.
Embodiment 33: the bark of eucommia: be seedling stage for the first time, in April, with 20 grams of nutrient substances, be watered 20 liters, evenly spray in one mu of bark of eucommia; Be the treelet phase for the second time, each spray of the annual 3-4 month and the 5-6 month once; For the third time for becoming the tree phase: each spray of the annual 4-5 month and the 7-8 month once.
Embodiment 34: golden cypress: be nursery stage for the first time, with 20 grams of nutrient substances, be watered 20 liters, evenly spray on one mu of golden cypress seedling; Be setting date for the second time, spray once; Be the patent medicine phase for the third time: each spray of the annual 4-5 month and the 7-8 month once.
Embodiment 35: peppermint: due to 1 year results several of peppermint, the turnout of plant is large, needs to consume larger nutrient, sprays for the first time as seedling growth stage, with 20 grams of nutrient substances, is watered 30 liters, evenly sprays on one mu of peppermint seedling; Spray for the second time as branch animated period: by embodiment 2 formulas, convert 30 premium on currency, evenly spray on one mu of peppermint seedling; Pregnant flower bud flowering period besides: press respectively embodiment 1, embodiment 2, embodiment 3 formulas in August, September, October, be watered respectively 20,30,40 liters, according to the growing state of peppermint in bud stage, initial bloom stage, each spray of full-bloom stage once.According in Nanping, Fujian, the first experimental result in circle, Anhui, can make peppermint oil offtake nearly double, simultaneously to improving the large containing the effect of alcohol amount of crude peppermint oil bu.
Embodiment 36: snakegourd: perennial herbaceous stem rattan wood, and from planting, the annual spray of Second Year is three times, in 30 centimetres of left and right of the climing length of stem, with 20 grams of nutrient substances, is watered 20 liters for the first time, evenly sprays on snakegourd rattan leaf; At the plant blossom initial stage, by embodiment 3 formulas, be watered 30 liters for the second time, evenly spray on one mu of snakegourd.At full-bloom stage, by embodiment 2 formulas, be watered 40 liters for the third time, evenly spray on one mu of snakegourd.Snakegourd can increase production 20%-30%, and the root (Snakegourd Root) of snakegourd also can increase production 30% left and right simultaneously.
Embodiment 37: cape jasmine: at initial bloom stage and full-bloom stage, spray once, with 20 grams of nutrient substances, be watered 30 liters, evenly spray on one mu of cape jasmine; At fruit development period and fruit expanding period, respectively spray once again: press embodiment 2 formulas, convert 30 premium on currency, evenly spray on one mu of cape jasmine; Can reduce shedding, increase output, improve quality.
Embodiment 38: Japanese Honeysuckle; Spray twice, for the first time also can initial bloom stage in the flower bud phase, with 30 grams of nutrient substances, be watered 30 liters, evenly spray on one mu of Japanese Honeysuckle.Full-bloom stage is watered 36 liters with 30 grams and evenly sprays on one mu of Japanese Honeysuckle for the second time.
Embodiment 39: Flos Rosae Chinensis; Spray twice, for the first time also can initial bloom stage in the flower bud phase, with 30 grams of nutrient substances, be watered 30 liters, evenly spray on one mu of Flos Rosae Chinensis.Full-bloom stage is watered 36 liters with 30 grams and evenly sprays on one mu of Flos Rosae Chinensis for the second time.
Embodiment 40: Inula Flower; Spray twice, for the first time also can initial bloom stage in the flower bud phase, with 30 grams of nutrient substances, be watered 30 liters, evenly spray on one mu of Inula Flower.Full-bloom stage is watered 36 liters with 30 grams and evenly sprays on one mu of Inula Flower for the second time.
Embodiment 41: Semen Lini: at initial bloom stage and full-bloom stage, spray once, with 20 grams of nutrient substances, be watered 30 liters, evenly spray in one mu of Semen Lini; At fruit development period and fruit expanding period, respectively spray once again: press embodiment 2 formulas, convert 30 premium on currency, evenly spray in one mu of Semen Lini; Can reduce shedding, increase output, improve quality.
Embodiment 42: tsaoko: at initial bloom stage and full-bloom stage, spray once, with 20 grams of nutrient substances, be watered 30 liters, evenly spray on one mu of tsaoko; At fruit development period and fruit expanding period, respectively spray once again: press embodiment 2 formulas, convert 30 premium on currency, evenly spray on one mu of tsaoko; Can reduce shedding, increase output, improve quality.
Embodiment 43: Semen Cassiae: at initial bloom stage and full-bloom stage, spray once, with 20 grams of nutrient substances, be watered 30 liters, evenly spray on one mu of Semen Cassiae; At fruit development period and fruit expanding period, respectively spray once again: press embodiment 2 formulas, convert 30 premium on currency, evenly spray on one mu of Semen Cassiae; Can reduce shedding, increase output, improve quality.
Embodiment 44: Siberian Cocklour Fruit: at initial bloom stage and full-bloom stage, spray once, with 20 grams of nutrient substances, be watered 30 liters, evenly spray on one mu of Siberian Cocklour Fruit; At fruit development period and fruit expanding period, respectively spray once again: press embodiment 2 formulas, convert 30 premium on currency, evenly spray on one mu of Siberian Cocklour Fruit; Can reduce shedding, increase output, improve quality.
Embodiment 45: wax gourd: be seedling stage for the first time, in April, with 20 grams of nutrient substances, be watered 20 liters, evenly spray on one mu of wax gourd; Be vegetative period for the second time, spray once; Be the achievement phase for the third time: spray once.
Embodiment 46: Pseudobulbus Cremastrae Seu Pleiones: from fruiting, within every 3 days, spray once, concentration 100-150ppm, increases production 55-87%, shorten collecting time 1/3rd.
Embodiment 47: Motherwort Herb: within every 3 days from emerging, spray once, concentration 100-150ppm, increases production 55-87%.
Claims (9)
1. the preparation method who contains the composition of nucleosides hydrochlorate and deoxynucleoside hydrochlorate, its spy levy in, composition is mainly comprised of nucleosides hydrochlorate and deoxynucleoside hydrochlorate, not sugary or/and amino acid, its preparation, take nucleic acid as raw material:
1., the hydrolysis of nucleic acid: the ratio mixed nucleus bronsted lowry acids and bases bronsted lowry liquid that is 1:20-30 according to nucleic acid, buck weight ratio, alkali lye is
5.5-7N, under 30-45 ℃ of condition, stirs 10-14 hours, obtains nucleosides hydrochlorate and deoxynucleoside acid salt solution, filters, and filtrate is adjusted pH8-8.5, concentrating under reduced pressure;
2., decolouring, dry concentrated solution is added to activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 10-25%;
3., crystallization dissolves nucleosides hydrochlorate and the deoxynucleoside hydrochlorate after dry with clear water, its solution adds alcohol separates out, and crystallization is taken out
Be filtered dry dryly, obtain white nucleosides hydrochlorate and deoxynucleoside hydrochlorate;
4., nucleosides hydrochlorate and deoxynucleoside hydrochlorate and water soluble mixt are pulverized, sieve.
2. according to the preparation method described in claim 1, it is characterized in that, in described nucleic acid hydrolysis process, alkali lye used is sodium hydroxide or potassium hydroxide solution.
3. preparation method according to claim 1, it is characterized in that, composition also comprises weighting agent: one or more of starch, dextrin, Mierocrystalline cellulose, polyvidone, Magnesium Stearate, calcium sulfate or magnesium sulfate, add the weighting agent of pulverizing to mix with nucleosides hydrochlorate and deoxynucleoside hydrochlorate, the weight percent of nucleosides hydrochlorate and deoxynucleoside hydrochlorate and weighting agent is: nucleosides hydrochlorate and deoxynucleoside hydrochlorate 10-40%, weighting agent 60-90%.
4. preparation method according to claim 1, is characterized in that: the consumption of described composition is every mu of 2-7 gram, by water-soluble water consumption, is every mu of 20-40 liter.
5. piece according to the preparation method described in claim 1,2 or 3, it is characterized in that: nucleosides hydrochlorate refers to: after Yeast Nucleic Acid basic hydrolysis, obtain: 5 '-adenylic acid (AMP) dipotassium or sodium, 5 '-bird adenylic acid (AMP) dipotassium or sodium, 5 '-cytidylic acid dipotassium or sodium, dipotassium or the disodium salt of 5 '-uridylic acid dipotassium or four kind 5 '-mixed nucleotides of sodium; Deoxynucleoside hydrochlorate refers to: after thymus nucleic acid basic hydrolysis, obtain: 5 '-Desoxyadenosine acid disodium or potassium, 5 '-deoxidation bird adenylic acid (AMP) disodium or potassium, 5 '-Deoxyribose cytidine acid disodium or potassium, 5 '-deoxyuridine acid disodium,, disodium salt or the di-potassium of five kind 5 '-deoxidation mixed nucleotides of 5 '-deoxythymidine acid disodium or potassium.
6. the preparation method who contains the composition of nucleosides hydrochlorate and deoxynucleoside hydrochlorate, its spy levy in, composition is mainly comprised of nucleosides hydrochlorate and deoxynucleoside hydrochlorate, not sugary or/and amino acid, its preparation: by weight percentage: nucleosides hydrochlorate 85-99%, deoxynucleoside hydrochlorate 1-15%, gets commercially available nucleosides hydrochlorate and deoxynucleoside hydrochlorate, mixes, pulverizes, sieves.
7. preparation method according to claim 6, it is characterized in that, composition also comprises weighting agent: one or more of starch, dextrin, Mierocrystalline cellulose, polyvidone, Magnesium Stearate, calcium sulfate or magnesium sulfate, add the weighting agent of pulverizing to mix with nucleosides hydrochlorate and deoxynucleoside hydrochlorate, the weight percent of nucleosides hydrochlorate and deoxynucleoside hydrochlorate and weighting agent is: nucleosides hydrochlorate and deoxynucleoside hydrochlorate 10-40%, weighting agent 60-90%.
8. preparation method according to claim 7, is characterized in that: the consumption of described composition is each every mu of 20-30 gram, by water-soluble water consumption, is every mu of 20-40 liter.
9. piece according to the preparation method described in claim 6,7 or 8, it is characterized in that: nucleosides hydrochlorate refers to: after Yeast Nucleic Acid basic hydrolysis, obtain: 5 '-adenylic acid (AMP) dipotassium or sodium, 5 '-bird adenylic acid (AMP) dipotassium or sodium, 5 '-cytidylic acid dipotassium or sodium, dipotassium or the disodium salt of 5 '-uridylic acid dipotassium or four kind 5 '-mixed nucleotides of sodium; Deoxynucleoside hydrochlorate refers to: after thymus nucleic acid basic hydrolysis, obtain: 5 '-Desoxyadenosine acid disodium or potassium, 5 '-deoxidation bird adenylic acid (AMP) disodium or potassium, 5 '-Deoxyribose cytidine acid disodium or potassium, 5 '-deoxyuridine acid disodium,, disodium salt or the di-potassium of five kind 5 '-deoxidation mixed nucleotides of 5 '-deoxythymidine acid disodium or potassium.
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| CN1948231A (en) * | 2005-10-13 | 2007-04-18 | 黑龙江禾芙康农药制造有限公司 | Formula of polypeptide liquid fertilizer and its preparation method |
| CN102311289A (en) * | 2010-07-02 | 2012-01-11 | 王祥林 | Organic water soluble fertilizer |
| CN102765978A (en) * | 2012-06-29 | 2012-11-07 | 占协琼 | Nutrient liquid for microorganisms |
| CN103351221A (en) * | 2010-10-21 | 2013-10-16 | 合肥凯歌信息科技有限公司 | Application of composition containing nucleotide salts and deoxynucleotide salts in plants |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1634806A (en) * | 2003-12-31 | 2005-07-06 | 武汉飞喜乐农业科技有限公司 | High-iron full-complex broad spectrum foliar fertilizer and method for making same |
| CN1948231A (en) * | 2005-10-13 | 2007-04-18 | 黑龙江禾芙康农药制造有限公司 | Formula of polypeptide liquid fertilizer and its preparation method |
| CN102311289A (en) * | 2010-07-02 | 2012-01-11 | 王祥林 | Organic water soluble fertilizer |
| CN103351221A (en) * | 2010-10-21 | 2013-10-16 | 合肥凯歌信息科技有限公司 | Application of composition containing nucleotide salts and deoxynucleotide salts in plants |
| CN102765978A (en) * | 2012-06-29 | 2012-11-07 | 占协琼 | Nutrient liquid for microorganisms |
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