CN103351193B - Good-quality and high-yield planting method for cotton - Google Patents
Good-quality and high-yield planting method for cotton Download PDFInfo
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- CN103351193B CN103351193B CN201310249567.8A CN201310249567A CN103351193B CN 103351193 B CN103351193 B CN 103351193B CN 201310249567 A CN201310249567 A CN 201310249567A CN 103351193 B CN103351193 B CN 103351193B
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Abstract
The invention discloses a planting method for cotton, which comprises application of a nutrient in a growth period of the cotton. The nutrient mainly comprises: nucleotide potassium salts, deoxynucleotide potassium salts and sugar. The time of the nutrient applied during the growth period is twice: the first time is in a bud stage or a flowering beginning stage, 30g of the nutrient is added with 30l of water and is sprayed uniformly on one mu of cotton, and the second time is in a full-bloom stage, 30g of the nutrient is added with 36l of water and is sprayed uniformly on one mu of cotton. The planting method of the invention has the advantages that the nutrient has no toxicity, harm, residue and any adverse side effect to the cotton; the nutrient is beneficial to increasing of the yield and the income and improving the efficiency and has good effects of enhancing the resistance and improving the quality for plants; the method has a simple process, the cost is low and large-scale production and promotion are easy.
Description
The application is application number 201010514217.6, the applying date: on October 21st, 2010, what denomination of invention was " nutrient substance of a kind of Promoting plant growth and preparation method thereof " divides an application.
Technical field
The present invention relates to an implantation methods growing cotton, particularly a good quality and high output implantation methods growing cotton.
Background technology
Greatly develop plant husbandry, forestry, promote that crop yield increases income, make our country green, it is the problem that various countries scientist pays close attention to research, the growth of plant be unable to do without and supplements the nutrients, and present plant husbandry, mainly by executing N,P,K fertilizers and organic fertilizer promotes crop growth, use too much fertilizer very large on the quality and flavor impact of farm crop, be unfavorable for development and food safety and the environmental protection of green agriculture.Therefore, people have studied growth hormone relevant to nucleic acid, nutrient substance, fertilizer.In theory: nucleic acid is the basic substance that forms life, is the basis of substance metabolism and energy metabolism, and the character of nucleic acid and the difference of quantity can cause the diversity of biological species and growth conditions.For congener life entity, the quantity of nucleic acid and the difference of existence form have determined prosperous the declining of power of this life entity.Therefore, nucleic acid substances has irreplaceable effect for the output raising in farm crop and even the production of whole vegitabilia and product quality improvement; Particularly several nucleotides salt and deoxynucleoside hydrochlorate and the cooperations thereof of nucleic acid small molecules such as nucleosides, Nucleotide, pyrimidine and purine, the not only growth of regulating plant very effectively, can be used as again nutrition source is absorbed rapidly by plant, strengthen plant root vigor, improve plant anti-adversity ability, thus improve crop output, improve product quality.Nucleic acid small molecules has enhancing improving activity of root system to plant, improves the absorption of root to phosphorus, potash fertilizer; Improve the activity of farm crop malic enzyme and succsinic acid enzyme, promote the assimilative capacity of plant, strengthen photosynthetic intensity, to plant-growth have regulate and trophism (Wang Xi etc. the research [J] of the application and function mechanism of nucleic acid degradation product in Rice Production. Acta Biochimica et Biophysica Sinica, 1975,7 (1): 31-39).Chinese patent literature (publication number: 101186534) disclose a kind of preparation method who contains nucleic acid degradation product foliage fertilizer, concrete step is: 1., lixiviate nuclease, 2., dissolve Yeast Nucleic Acid (alkali lye hydrotropy), 3., the hydrolysis of nucleic acid, must contain
There are the multiple nucleic acids small molecules such as nucleosides, Nucleotide, purine, pyrimidine and a small amount of reducing sugar and few big or middle, trace element, the method be take Yeast Nucleic Acid as raw material, with enzymatic hydrolysis, exist production cost high, degrade not thorough, seldom, containing deoxynucleotide composition, in mixed system, Nucleotide salt yield is low, the mutual mixing ratio imbalance of various nucleosides hydrochlorates and deoxynucleotide salt face is very large on result of use impact.Chinese patent literature (publication number: 101050143) disclose a kind of nutrient substance that promotes crop growth, it is comprised of ribavirin and glucose, production and the guanosine of ribavirin are in close relations, and this patent exists ribavirin high cost, the unconspicuous problem of practical effect; Chinese patent literature (publication number: the preparation method who 1238323) discloses a kind of nucleic acid foliage fertilizer, the degraded of its nucleic acid adopts the method for alkaline hydrolysis, degradation product is purine and pyrimidine, there is equally nucleic acid high cost in this patent, various nucleosides hydrochlorates and the mutual mixing ratio of deoxynucleotide salt face are unreasonable, affect the problem of result of use.
Summary of the invention
The object of this invention is to provide an implantation methods growing cotton, its production cost is low, and method is simple, is easy to large-scale production and popularization; In mixed system, Nucleotide salt yield is high, and various nucleosides hydrochlorates and the mutual mixing ratio of deoxynucleotide salt face are reasonable, nontoxic, harmless, noresidue, without any adverse side effect; Increase production, increase income, synergy, strengthen resistance, improve quality effective.
The present invention realizes like this.
The implantation methods of cotton, during cotton growth, uses nutrient substance; Described nutrient substance is mainly comprised of nucleosides hydrochlorate and deoxynucleoside hydrochlorate, sugar, its weight percent is: nucleosides hydrochlorate and deoxynucleoside hydrochlorate 10-30%, sugar 70-90%, sugar in described nutrient substance is glucose, and to be the mycelium of take after the fermentation plant fermentation nucleic acid that adopts dry method or wet production make according to the following steps as raw material for the nucleosides hydrochlorate in described nutrient substance and deoxynucleoside hydrochlorate.
1., the hydrolysis of the nucleic acid ratio mixed nucleus bronsted lowry acids and bases bronsted lowry liquid that is 1:20-30 according to nucleic acid, buck weight ratio, alkali lye is 5.5-7N, under 30-45 ℃ of condition, stir 10-14 hours, obtain nucleosides hydrochlorate and deoxynucleoside acid salt solution, filter, filtrate is adjusted pH8-8.5, concentrating under reduced pressure.
2., decolouring, dry concentrated solution is added to activated carbon decolorizing, suction filtration, filtrate decompression is concentrated into moisture 10-25%.
3., crystallization dissolves nucleosides hydrochlorate and the deoxynucleoside hydrochlorate after dry with clear water, its solution adds alcohol separates out, and crystallization suction filtration is dry, the white nucleosides hydrochlorate and deoxynucleoside hydrochlorate and the water soluble mixt that obtain.
4., nucleosides hydrochlorate and deoxynucleoside hydrochlorate and water soluble mixt are pulverized, cross 200 mesh sieves and mix with sugar again.
Time and usage quantity that described growing period is used nutrient substance are: spray twice.
At flower bud phase or initial bloom stage, with 30 grams of nutrient substances, be watered 30 liters for the first time, evenly spray on one mu of cotton.
Be full-bloom stage for the second time, with 30 grams of nutrient substances, be watered 36 liters, evenly spray on one mu of cotton.
In nucleic acid hydrolysis process described in this preparation method, alkali lye used is sodium hydroxide or potassium hydroxide solution.
In the present invention, nucleosides hydrochlorate mainly refers to: 5 '-adenylic acid (AMP) disodium or potassium, 5 '-bird adenylic acid (AMP) disodium or potassium, 5 '-cytidylic acid disodium or potassium, sodium salt or the sylvite of four kind 5 '-mixed nucleotides of 5 '-uridine monophosphate disodium or potassium; Deoxynucleoside hydrochlorate refers to: 5 '-Desoxyadenosine acid disodium or potassium, 5 '-deoxidation bird adenylic acid (AMP) disodium or potassium, 5 '-Deoxyribose cytidine acid disodium or potassium, 5 '-deoxyuridine acid disodium,, sodium salt or the sylvite of five kind 5 '-deoxidation mixed nucleotides of 5 '-deoxythymidine acid disodium or potassium.The source of nucleosides hydrochlorate and deoxynucleoside hydrochlorate is for take nucleic acid as raw material, mainly that to adopt the nucleic acid of dry method or wet production be raw material for mycelium from fermentation plant fermentation, this kind of nucleic acid be take Yeast Nucleic Acid (RNA) as a small amount of thymus nucleic acid of main mixing (DNA), with alkali lye hydrolysis, makes; Auxiliary agent is mainly derived from two aspects: 1, the nucleic acid of dry method or wet production also contains the impurity such as sugar, protein, various metals or nonmetallic ion, has amino acid, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc. after hydrolysis; 2, exogenous adding: sugar (reducing sugar, non-reducing sugar: as glucose, sucrose also have fructose etc.), amino acid, big or middle, micro-etc.
The present invention adopt mainly by nucleosides hydrochlorate and deoxynucleoside hydrochlorate, the Promoting plant growth nutrient substance that auxiliary agent forms and for take nucleic acid as raw material, mainly from fermentation plant (Gourmet Powder Factory, brew-house, antibiotics plant, yeast factory, Amino Acid Factory, paper mill, active sludge after sewage disposal, petroleum fermentations etc.) nucleic acid of the employing dry method of byproduct (tankage) mycelium after fermentation or wet production is the preparation method of raw material hydrolysis, with currently available products and the method (product that adds ribavirin, the product being directly hydrolyzed with Yeast Nucleic Acid, enzyme solution) compare and have the following advantages: 1, it is low that mycelium from fermentation plant fermentation is produced its production cost of Yeast Nucleic Acid, be easy to large-scale production and popularization, 2, the nucleic acid that mycelium is produced is containing useful impurity, in addition basic hydrolysis method, technique is simple, in mixed system, Nucleotide salt yield is high, and various nucleosides hydrochlorates and the mutual mixing ratio of deoxynucleotide salt face are reasonable, and amino acid, sugar part and other organic-inorganic auxiliary agent content are higher, therefore, increasing both production and income synergy, strengthens resistance, improves quality effective, 3,, nontoxic, harmless, noresidue, without any adverse side effect, 4, be widely used in grain, cotton, oil, cigarette, tea, really, plant and the bacterium mushroom series products such as dish, Chinese medicinal materials, flowers, forest.
Embodiment
Embodiment 1: get 1 gram of nucleosides hydrochlorate and deoxynucleoside hydrochlorate, and 6 grams of glucose, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 2: get 1 gram of nucleosides hydrochlorate and deoxynucleoside hydrochlorate, and 2 grams of sucrose, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 3: get 2 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, 8 grams of glucose, nucleosides hydrochlorate and de-
Oxygen nucleosides hydrochlorate is pulverized, and mixes with glucose, obtains nutrient substance.
Embodiment 4: get 1.5 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, and 8.5 grams of glucose, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 5: get 1 gram of nucleosides hydrochlorate and deoxynucleoside hydrochlorate, and 9 grams of glucose, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 6: get 2.5 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, and 7.5 grams of glucose, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 7: get 4 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, and 6 grams, fructose, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 8: get 5 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, and 5 grams of glucose, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 9: get 1 gram, Nucleotide potassium and deoxynucleotide potassium, and 9 grams of glucose, Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 10: get 1.5 grams, Nucleotide potassium and deoxynucleotide potassium, and 8.5 grams of glucose, Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 11: get 2 grams, Nucleotide potassium and deoxynucleotide potassium, and 8 grams of glucose, Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 12: get 2.5 grams, Nucleotide potassium and deoxynucleotide potassium, and 7.5 grams of glucose, Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 13: get 3 grams, Nucleotide potassium and deoxynucleotide potassium, and 7 grams of glucose, Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 14: get 1.5 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, and 4.5 grams of glucose, 4 grams, amino acid, nucleosides hydrochlorate and deoxynucleoside hydrochlorate and amino acid are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 15: get 2 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, and 4 grams of glucose, 4 grams, amino acid, nucleosides hydrochlorate and deoxynucleoside hydrochlorate and amino acid are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 16: get 3 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, and 4 grams of glucose, 3 grams, amino acid, nucleosides hydrochlorate and deoxynucleoside hydrochlorate and amino acid are pulverized, and mix with glucose, obtain nutrient substance.
Embodiment 17: get 2 grams of nucleosides hydrochlorate and deoxynucleoside hydrochlorates, and 8 grams, amino acid, nucleosides hydrochlorate and deoxynucleoside hydrochlorate and amino acid are pulverized, and mix, and obtain nutrient substance.
Embodiment 18: by 100 grams, the crude product Yeast Nucleic Acid (RNA) making from the mycelium of fermentation plant's byproduct by dry method or wet method, be dissolved in the sodium hydroxide solution of 3 liters of 6.3N, under 37 ℃ of conditions, stir 12 hours, winner will refer to and contain: 5 '-adenylic acid (AMP) disodium, 5 '-bird adenylic acid (AMP) disodium, 5 '-cytidylic acid disodium, the sodium salt of four kind 5 '-mixed nucleotides of 5 '-uridine monophosphate disodium (also has deoxynucleoside hydrochlorate: 5 '-Desoxyadenosine acid disodium, 5 '-deoxidation bird adenylic acid (AMP) disodium, 5 '-Deoxyribose cytidine acid disodium, 5 '-deoxyuridine acid disodium, , sodium salt and the amino acid of five kind 5 '-deoxidation mixed nucleotides of 5 '-deoxythymidine acid disodium, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc.) solution, filter, filtrate is neutralized to pH8-8.5, be evaporated to appropriate, concentrated solution is added to activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 15%, by proper amount of clear water, dissolve nearly dry new-nucleo and Sodium Deoxyribomuleotide, its solution adds alcohol and separates out, crystallization suction filtration is dry, approximately obtain 85-90 grams, white nucleosides hydrochlorate and deoxynucleoside hydrochlorate and a certain amount of helping and agent water soluble mixt, gains are pulverized, crossed 200 mesh sieves, mix with auxiliary agent (while again adding auxiliary agent).
Embodiment 19: by 100 grams, the crude product Yeast Nucleic Acid (RNA) making from the mycelium of fermentation plant's byproduct by dry method or wet method, be dissolved in the potassium hydroxide solution of 2.5 liter of 6 N, under 40 ℃ of conditions, stir 10 hours, winner will refer to and contain: 5 '-adenylic acid (AMP) dipotassium, 5 '-bird adenylic acid (AMP) dipotassium, 5 '-cytidylic acid dipotassium, the sylvite of four kind 5 '-mixed nucleotides of 5 '-uridylic acid dipotassium (also has deoxynucleoside hydrochlorate: 5 '-deoxyadenylic acid dipotassium, 5 '-deoxidation bird adenylic acid (AMP) dipotassium, 5 '-deoxycytidylic acid(dCMP) dipotassium, 5 '-
Deoxyuridylic acid dipotassium,, the sylvite of five kind 5 '-deoxidation mixed nucleotides of 5 '-deoxythymidylic acid dipotassium and amino acid, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc.) and solution, filters, filtrate is neutralized to pH8-8.5, is evaporated to appropriate; Concentrated solution is added to activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into 20%; By proper amount of clear water, dissolve nearly dry Nucleotide potassium and deoxynucleotide potassium, its solution adds alcohol separates out, and crystallization suction filtration is dry, approximately obtains 88-93 grams of white Nucleotide sylvite and deoxynucleoside acid potassium salt and a certain amount of auxiliary agent and water soluble mixt.Gains are pulverized, crossed 200 mesh sieves, mix with auxiliary agent (while again adding auxiliary agent).
Embodiment 20: paddy rice: spray twice, for initial bloom stage (heading 10% left and right) is watered 30 liters with 20 grams of nutrient substances (containing glucose 80-85%'s), concentration 100ppm left and right, evenly sprays on one mu of paddy rice for the first time.Be full-bloom stage for the second time, within 5-6 days after spraying for the first time, with 20 grams of nutrient substances, be watered 30 liters, evenly spray on one mu of paddy rice and get final product (70-100 kilograms of mu volume increase, volume increase 15% left and right).
Embodiment 21: cotton: spray twice, for the first time also can initial bloom stage in the flower bud phase, and with 30 grams of nutrient substances, be watered 30 liters, evenly spray on one mu of cotton.Full-bloom stage is watered 36 liters with 30 grams evenly spray on one mu of cotton can (mu volume increase be more than 7%) for the second time.
Embodiment 22: rape: spraying twice, is initial bloom stage for the first time, be watered 20 liters spray on one mu of rape with 20 grams of nutrient substances.Be full-bloom stage for the second time, be watered 30 liters with 20 grams, spray on one mu of rape, volume increase 15-20 kilogram.
Embodiment 23: tobacco: spray twice, with 30 grams, be often watered 30 kilograms, concentration 200ppm left and right at every turn, spray on one mu of tobacco leaf, spray and carry out 20-26 day in time in May, (the south north time is slightly different), sprays for the first time, 3-6 day in June sprays for the second time, concentration 150ppm left and right, quality improves a grade, volume increase 20% left and right.
Embodiment 24: tealeaves: at growth germination period, spray for the first time, after every harvesting, spray once, concentration 180ppm left and right.
Embodiment 25: capsicum: initial bloom stage and full-bloom stage each once, concentration 200-300ppm, mu volume increase 40%～80%.
Embodiment 26: strawberry: in the fruitlet phase, spray, be watered 18 liters spray with 10 grams, spray 3～4 times in batches and can increase production 38% after harvesting.
Embodiment 27: Chinese medicinal materials: the Chinese medicinal materials of different sites, using method differs, generally initial bloom stage and full-bloom stage each once, concentration 100-260ppm.
Embodiment 28: mushroom: from fruiting, within every 3 days, spray once, and concentration 100-150ppm, volume increase 50-80%, shortens collecting time 1/3rd.
Claims (1)
1. the implantation methods of cotton, is characterized in that: during cotton growth, use nutrient substance; Described nutrient substance is mainly comprised of nucleosides hydrochlorate and deoxynucleoside hydrochlorate, sugar, its weight percent is: nucleosides hydrochlorate and deoxynucleoside hydrochlorate 10-30%, sugar 70-90%, sugar in described nutrient substance is glucose, and to be the mycelium of take after the fermentation plant fermentation nucleic acid that adopts dry method or wet production make according to the following steps as raw material for the nucleosides hydrochlorate in described nutrient substance and deoxynucleoside hydrochlorate:
1., the hydrolysis of the nucleic acid ratio mixed nucleus bronsted lowry acids and bases bronsted lowry liquid that is 1:20-30 according to nucleic acid, buck weight ratio, alkali lye is 5.5-7N, under 30-45 ℃ of condition, stir 10-14 hours, obtain nucleosides hydrochlorate and deoxynucleoside acid salt solution, filter, filtrate is adjusted pH8-8.5, concentrating under reduced pressure;
2., decolouring, dry concentrated solution is added to activated carbon decolorizing, suction filtration, filtrate decompression is concentrated into moisture 10-25%;
3., crystallization dissolves nucleosides hydrochlorate and the deoxynucleoside hydrochlorate after dry with clear water, its solution adds alcohol separates out, and crystallization suction filtration is dry, the white nucleosides hydrochlorate and deoxynucleoside hydrochlorate and the water soluble mixt that obtain;
4., nucleosides hydrochlorate and deoxynucleoside hydrochlorate and water soluble mixt are pulverized, cross 200 mesh sieves and mix with sugar again;
Time and usage quantity that described growing period is used nutrient substance are: spray twice, for the first time at flower bud phase or initial bloom stage, with 30 grams of nutrient substances, be watered 30 liters, evenly spray on one mu of cotton, be full-bloom stage for the second time, with 30 grams of nutrient substances, be watered 36 liters, evenly spray on one mu of cotton.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310249567.8A CN103351193B (en) | 2010-10-21 | 2010-10-21 | Good-quality and high-yield planting method for cotton |
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| CN201310249567.8A CN103351193B (en) | 2010-10-21 | 2010-10-21 | Good-quality and high-yield planting method for cotton |
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| CN 201010514217 Division CN102079665B (en) | 2010-10-21 | 2010-10-21 | Nutrient for enhancing plant growth and preparation method thereof |
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| CN103351193A CN103351193A (en) | 2013-10-16 |
| CN103351193B true CN103351193B (en) | 2014-12-10 |
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| CN102146005B (en) * | 2010-11-25 | 2014-12-10 | 高旌 | Application method of auxin |
| CN105272533A (en) * | 2013-11-19 | 2016-01-27 | 安徽天之正生物科技有限公司 | Production method of Divaricate Saposhnikovia Root |
| CN115399107B (en) * | 2022-06-10 | 2023-09-15 | 华中农业大学 | Application of glucose in cotton planting and initiator |
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| CN1317943C (en) * | 2004-08-31 | 2007-05-30 | 北京科欣基业科技发展有限公司 | Cultivating method for super early mature short season cotton |
| AU2007200687B2 (en) * | 2006-02-23 | 2009-01-29 | Van Schaik's Bio Gro Pty Ltd | A seagrass based nutrient product for plant biological growth stimulation and a method for preparing the same |
| CN1911869A (en) * | 2006-08-25 | 2007-02-14 | 陈俊茂 | Leaf fertilizer containing amino acid and its preparation method |
| CN100556863C (en) * | 2007-03-26 | 2009-11-04 | 蔡公望 | A kind of nutrient substance that promotes crop growth |
| CN101642020B (en) * | 2008-08-08 | 2012-04-18 | 颜平德 | High-yield method for cultivating wheat, corn, cotton and rape by improving plant forms |
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