CN103348843B - Planting method of strawberries - Google Patents
Planting method of strawberries Download PDFInfo
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- CN103348843B CN103348843B CN201310249560.6A CN201310249560A CN103348843B CN 103348843 B CN103348843 B CN 103348843B CN 201310249560 A CN201310249560 A CN 201310249560A CN 103348843 B CN103348843 B CN 103348843B
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- hydrochlorate
- nutrient
- deoxyribonucleoside
- nucleosides
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- 238000000034 method Methods 0.000 title claims abstract description 35
- 235000021012 strawberries Nutrition 0.000 title abstract 2
- 240000009088 Fragaria x ananassa Species 0.000 title 1
- 235000015097 nutrients Nutrition 0.000 claims abstract description 48
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 27
- 150000001413 amino acids Chemical class 0.000 claims abstract description 23
- 241000220223 Fragaria Species 0.000 claims abstract description 15
- 235000016623 Fragaria vesca Nutrition 0.000 claims abstract description 14
- 235000011363 Fragaria x ananassa Nutrition 0.000 claims abstract description 14
- 150000003839 salts Chemical class 0.000 claims abstract description 9
- 239000005549 deoxyribonucleoside Substances 0.000 claims description 52
- 239000002777 nucleoside Substances 0.000 claims description 52
- 125000003835 nucleoside group Chemical group 0.000 claims description 51
- 229910052700 potassium Inorganic materials 0.000 claims description 39
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 38
- 239000011591 potassium Substances 0.000 claims description 38
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 35
- 239000008103 glucose Substances 0.000 claims description 35
- 102000039446 nucleic acids Human genes 0.000 claims description 28
- 108020004707 nucleic acids Proteins 0.000 claims description 28
- 150000007523 nucleic acids Chemical class 0.000 claims description 28
- 239000007921 spray Substances 0.000 claims description 26
- 239000000203 mixture Substances 0.000 claims description 25
- 239000002773 nucleotide Substances 0.000 claims description 25
- 239000002253 acid Substances 0.000 claims description 20
- 241000196324 Embryophyta Species 0.000 claims description 18
- 238000004519 manufacturing process Methods 0.000 claims description 15
- 238000000855 fermentation Methods 0.000 claims description 14
- 230000004151 fermentation Effects 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 238000002513 implantation Methods 0.000 claims description 11
- 239000000243 solution Substances 0.000 claims description 11
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 10
- 239000000706 filtrate Substances 0.000 claims description 10
- 238000000967 suction filtration Methods 0.000 claims description 10
- 159000000000 sodium salts Chemical class 0.000 claims description 9
- 238000002425 crystallisation Methods 0.000 claims description 7
- 230000008025 crystallization Effects 0.000 claims description 7
- 230000007062 hydrolysis Effects 0.000 claims description 7
- 238000006460 hydrolysis reaction Methods 0.000 claims description 7
- 239000002994 raw material Substances 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 5
- 241000218636 Thuja Species 0.000 claims description 5
- 239000003513 alkali Substances 0.000 claims description 5
- 239000012141 concentrate Substances 0.000 claims description 5
- -1 filter Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 239000002585 base Substances 0.000 claims description 3
- 238000003306 harvesting Methods 0.000 claims description 3
- 150000007516 brønsted-lowry acids Chemical class 0.000 claims description 2
- 150000007528 brønsted-lowry bases Chemical class 0.000 claims description 2
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 239000012266 salt solution Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 8
- 230000012010 growth Effects 0.000 abstract description 8
- 230000002411 adverse Effects 0.000 abstract description 3
- 230000001965 increasing effect Effects 0.000 abstract description 3
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 3
- 231100000252 nontoxic Toxicity 0.000 abstract description 3
- 230000003000 nontoxic effect Effects 0.000 abstract description 3
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 21
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 17
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 12
- 229920002477 rna polymer Polymers 0.000 description 11
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 8
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 7
- KCIDZIIHRGYJAE-YGFYJFDDSA-L dipotassium;[(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] phosphate Chemical compound [K+].[K+].OC[C@H]1O[C@H](OP([O-])([O-])=O)[C@H](O)[C@@H](O)[C@H]1O KCIDZIIHRGYJAE-YGFYJFDDSA-L 0.000 description 7
- 239000003337 fertilizer Substances 0.000 description 6
- 239000012535 impurity Substances 0.000 description 6
- 229910052698 phosphorus Inorganic materials 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 5
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 5
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 5
- 239000012752 auxiliary agent Substances 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- 239000011574 phosphorus Substances 0.000 description 5
- 239000001103 potassium chloride Substances 0.000 description 5
- 235000011164 potassium chloride Nutrition 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 4
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 4
- 229920000742 Cotton Polymers 0.000 description 4
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 4
- 240000007594 Oryza sativa Species 0.000 description 4
- 235000007164 Oryza sativa Nutrition 0.000 description 4
- IWUCXVSUMQZMFG-AFCXAGJDSA-N Ribavirin Chemical compound N1=C(C(=O)N)N=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](CO)O1 IWUCXVSUMQZMFG-AFCXAGJDSA-N 0.000 description 4
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 4
- 239000006227 byproduct Substances 0.000 description 4
- 229910052791 calcium Inorganic materials 0.000 description 4
- 239000011575 calcium Substances 0.000 description 4
- 229910052802 copper Inorganic materials 0.000 description 4
- 239000010949 copper Substances 0.000 description 4
- 229910052742 iron Inorganic materials 0.000 description 4
- 239000011777 magnesium Substances 0.000 description 4
- 229910052749 magnesium Inorganic materials 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 230000008635 plant growth Effects 0.000 description 4
- 229960000329 ribavirin Drugs 0.000 description 4
- HZCAHMRRMINHDJ-DBRKOABJSA-N ribavirin Natural products O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1N=CN=C1 HZCAHMRRMINHDJ-DBRKOABJSA-N 0.000 description 4
- 235000009566 rice Nutrition 0.000 description 4
- 239000011701 zinc Substances 0.000 description 4
- 229910052725 zinc Inorganic materials 0.000 description 4
- MXFFCFOTFSPXOE-JXOAFFINSA-N 1-[(2r,3r,4s,5r)-3,4-dihydroxy-5-methyloxolan-2-yl]-5-methylpyrimidine-2,4-dione Chemical compound O[C@@H]1[C@H](O)[C@@H](C)O[C@H]1N1C(=O)NC(=O)C(C)=C1 MXFFCFOTFSPXOE-JXOAFFINSA-N 0.000 description 3
- HLPAJQITBMEOML-XVFCMESISA-N 4-amino-1-[(2r,3r,4s,5r)-3,4-dihydroxy-5-methyloxolan-2-yl]pyrimidin-2-one Chemical compound O[C@@H]1[C@H](O)[C@@H](C)O[C@H]1N1C(=O)N=C(N)C=C1 HLPAJQITBMEOML-XVFCMESISA-N 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 3
- 239000005715 Fructose Substances 0.000 description 3
- 229930091371 Fructose Natural products 0.000 description 3
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 3
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- PNPLHKHBANFGMS-WFIJOQBCSA-N [Na].[Na].[C@@H]1([C@H](O)[C@H](O)[C@@H](COP(=O)(O)O)O1)N1C(=O)N=C(N)C=C1 Chemical compound [Na].[Na].[C@@H]1([C@H](O)[C@H](O)[C@@H](COP(=O)(O)O)O1)N1C(=O)N=C(N)C=C1 PNPLHKHBANFGMS-WFIJOQBCSA-N 0.000 description 3
- 239000012043 crude product Substances 0.000 description 3
- 239000007857 degradation product Substances 0.000 description 3
- KURVIXMFFSNONZ-WFIJOQBCSA-L disodium;[(2r,3s,4r,5r)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound [Na+].[Na+].O[C@@H]1[C@H](O)[C@@H](COP([O-])([O-])=O)O[C@H]1N1C(=O)NC(=O)C=C1 KURVIXMFFSNONZ-WFIJOQBCSA-L 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 229910052748 manganese Inorganic materials 0.000 description 3
- 239000011572 manganese Substances 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- 229940061671 uridine 5-mo-phos disod Drugs 0.000 description 3
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 2
- 244000061176 Nicotiana tabacum Species 0.000 description 2
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 2
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 description 2
- 238000005903 acid hydrolysis reaction Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000005547 deoxyribonucleotide Substances 0.000 description 2
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 235000002566 Capsicum Nutrition 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 description 1
- NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 description 1
- 102000013460 Malate Dehydrogenase Human genes 0.000 description 1
- 108010026217 Malate Dehydrogenase Proteins 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- WIXIBOVGGNRVQH-WFIJOQBCSA-N [K].[K].[C@@H]1([C@H](O)[C@H](O)[C@@H](COP(=O)(O)O)O1)N1C(=O)N=C(N)C=C1 Chemical compound [K].[K].[C@@H]1([C@H](O)[C@H](O)[C@@H](COP(=O)(O)O)O1)N1C(=O)N=C(N)C=C1 WIXIBOVGGNRVQH-WFIJOQBCSA-N 0.000 description 1
- VHUKPXJCIYLZCH-WFIJOQBCSA-N [K].[K].[C@@H]1([C@H](O)[C@H](O)[C@@H](COP(=O)(O)O)O1)N1C(=O)NC(=O)C=C1 Chemical compound [K].[K].[C@@H]1([C@H](O)[C@H](O)[C@@H](COP(=O)(O)O)O1)N1C(=O)NC(=O)C=C1 VHUKPXJCIYLZCH-WFIJOQBCSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000005904 alkaline hydrolysis reaction Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 235000021028 berry Nutrition 0.000 description 1
- 239000001390 capsicum minimum Substances 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 235000019504 cigarettes Nutrition 0.000 description 1
- 239000000039 congener Substances 0.000 description 1
- JSRLJPSBLDHEIO-SHYZEUOFSA-N dUMP Chemical compound O1[C@H](COP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 JSRLJPSBLDHEIO-SHYZEUOFSA-N 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
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- 230000037149 energy metabolism Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 229940029575 guanosine Drugs 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 229940072033 potash Drugs 0.000 description 1
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 description 1
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 1
- 235000015320 potassium carbonate Nutrition 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 125000000561 purinyl group Chemical group N1=C(N=C2N=CNC2=C1)* 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000001384 succinic acid Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Saccharide Compounds (AREA)
Abstract
The invention discloses a planting method of strawberries with good quality and high yield. The method comprises the steps as follows: nutrient is applied during the strawberry growth period; the nutrient mainly comprises components in percentage by weight as follows: 10%-80% of nucleotide salt and deoxynucleotide salt and 20%-90% of sugar or/ and amino acid; and the time of applying the nutrient during the growth period is that the nutrient is sprayed once during the fruitlet period, and sprayed for 3-4 times in batches after picking is finished. The planting method has the advantages that the nutrient is non-toxic and harmless, and has no residue and no adverse side effect; yield, income and efficiency are increased, resistance is enhanced, and an effect of improving quality is good; and further, the method is simple, low in cost and prone to large-scale production and popularization.
Description
The application is application number 201010514217.6, the applying date: on October 21st, 2010, and denomination of invention is the divisional application of " nutrient of a kind of Promoting plant growth and preparation method thereof ".
Technical field
The present invention relates to a kind of implantation methods of berry, particularly the implantation methods of a kind of strawberry.
Background technology
Greatly develop plant husbandry, forestry, promote that crop yield increases income, make our country green, it is the problem that various countries scientist pays close attention to research, the growth of plant be unable to do without and supplements the nutrients, present plant husbandry, mainly by executing N,P,K fertilizers and organic manure to promote crop growth, use too much fertilizer very large on the impact of the quality and flavor of crops, be unfavorable for the development of green agriculture and food security and environmental protection.Therefore, people have studied and nucleic acid related growth element, nutrient, fertilizer.In theory: nucleic acid is the basic substance forming life, and be the basis of metabolism and energy metabolism, the character of nucleic acid and the difference of quantity can cause the diversity of biological species and growth conditions.For congener life entity, the quantity of nucleic acid and the difference of existence form determine that the power of this life entity is prosperous to decline.Therefore, nucleic acid substances improves for the output increased in crops and even the production of whole vegetative kingdom and product quality irreplaceable effect; The nucleic acid Small molecular such as nucleosides, nucleotide, pyrimidine and purine are several nucleotides salt and deoxyribonucleoside hydrochlorate and cooperation thereof particularly, not only can the growth of effectively regulating plant, can be absorbed rapidly by plant as nutrient source again, strengthen plant root vigor, improve plant anti-adversity ability, thus improve crop output, improve product quality.Nucleic acid Small molecular has enhancing improving activity of root system to plant, improves root to the absorption of phosphorus, potash fertilizer; Improve the activity of crops malate dehydrogenase and succinic acid enzyme, promote the assimilational capacity of plant, strengthen photosynthetic intensity, to plant growth have adjustment and trophism (Wang Xi etc. the research [J] of the application and function mechanism of nucleic acid degradation product in Rice Production. Acta Biochimica et Biophysica Sinica, 1975,7 (1): 31-39).3., the hydrolysis of nucleic acid 1., lixiviate nuclease (publication number: 101186534) disclose a kind of preparation method containing nucleic acid degradation product foliage fertilizer, concrete step is Chinese patent literature:, 2., dissolves ribonucleic acid (alkali lye hydrotropy), must contain
There are the multiple nucleic acids Small molecular such as nucleosides, nucleotide, purine, pyrimidine and a small amount of reducing sugar and few large, middle and trace element, the method take ribonucleic acid as raw material, with enzymatic hydrolysis, there is production cost high, degraded is not thorough, and few containing deoxynucleotide composition, mixed system nucleotide salt yield is low, various nucleosides hydrochlorate and the mutual mixing ratio imbalance of deoxynucleotide salt face are very large on result of use impact.Chinese patent literature (publication number: 101050143) disclose a kind of nutrient promoting crop growth, it is made up of Ribavirin and glucose, production and the guanosine of Ribavirin are in close relations, and this patent exists Ribavirin high cost, the unconspicuous problem of practical effect; Chinese patent literature (publication number: the preparation method 1238323) disclosing a kind of nucleic acid foliage fertilizer, the degraded of its nucleic acid adopts the method for alkaline hydrolysis, degradation product is purine and pyrimidine, there is nucleic acid high cost equally in this patent, various nucleosides hydrochlorate and the mutual mixing ratio of deoxynucleotide salt face unreasonable, affect the problem of result of use.
Summary of the invention
The object of this invention is to provide the implantation methods of a kind of strawberry, its production cost is low, and method is simple, is easy to large-scale production and popularization; Mixed system nucleotide salt yield is high, and rationally, nontoxic, harmless, noresidue, without any adverse side effect for various nucleosides hydrochlorate and the mutual mixing ratio of deoxynucleotide salt face; Increase production, increase income, synergy, strengthen resistance, improve quality effective.
The present invention is achieved in that
The implantation methods of strawberry, at strawberry growing period, uses nutrient; Described nutrient is primarily of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, sugar or amino acid composition, its percentage by weight is: nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 10-30%, sugar or amino acid 70-90%, the nucleosides hydrochlorate in described nutrient and deoxyribonucleoside hydrochlorate be with fermentation plant fermentation after mycelium adopt the nucleic acid of dry method or wet production for raw material:
1., the hydrolysis of nucleic acid is the ratio mixed nucleus bronsted lowry acids and bases bronsted lowry liquid of 1:20-30 according to nucleic acid, buck weight ratio, and alkali lye is 5.5-7N, under 30-45 DEG C of condition, stir 10-14 hours, obtain nucleosides hydrochlorate and deoxyribonucleoside acid salt solution, filter, filtrate adjusts pH8-8.5, reduced pressure concentration;
2., decolouring, dry concentrate is added activated carbon decolorizing, suction filtration it, filtrate reduced in volume is to moisture 10-25%;
3., crystallization clear water dissolves near dry nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, and its solution adds alcohol and separates out, and crystallization suction filtration is dry, the white core thuja acid salt obtained and deoxyribonucleoside hydrochlorate and water soluble mixt;
4., by nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and water soluble mixt pulverize, cross 200 mesh sieves and mix with sugar or amino acid again;
The time that described growing period uses nutrient is: spray once in the fruitlet phase, sprays 3 ~ 4 times after harvesting in batches.
The implantation methods of strawberry, the consumption using nutrient is: be watered 18 liters with 10 grams and spray.
The implantation methods of strawberry, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are nucleotide potassium or sodium salt and deoxynucleotide potassium or sodium salt.
Sugar in described nutrient is glucose or sucrose or fructose.
In the nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate of described nutrient, percentage by weight is: nucleosides hydrochlorate 85-99%, deoxyribonucleoside hydrochlorate 1-15%.
Described nutrient is 100 grams, the crude product ribonucleic acid obtained from the mycelium of fermentation plant's by product by dry method or wet method, be dissolved in the sodium hydroxide solution of 3 liters of 6.3N, under 37 DEG C of conditions, stir 12 hours, obtain: the sodium salt of four kinds of 5 '-mixed nucleotides: 5 '-adenylate disodium, 5 '-bird adenylate disodium, 5 '-cytidylic acid disodium, 5 '-uridine monophosphate disodium, also have five kinds of 5 '-deoxidation mixed nucleotides sodium salts: 5 '-desoxyadenossine acid disodium, 5 '-deoxidation bird adenylate disodium, 5 '-deoxycytidine acid disodium, 5 '-BrdU acid disodium, , 5 '-deoxyribosylthymine acid disodium and impurity: amino acid, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, the solution of manganese, filter, filtrate is neutralized to pH8-8.5, be evaporated to appropriate, concentrate is added activated carbon decolorizing, suction filtration it, filtrate reduced in volume is to moisture 15%, nearly dry new-nucleo and deoxyribonucleotide natrium is dissolved by proper amount of clear water, its solution adds alcohol and separates out, crystallization suction filtration is dry, obtain 90 grams, white core thuja acid salt and deoxyribonucleoside hydrochlorate and a certain amount ofly to help and agent water soluble mixt, gains are pulverized, crosses 200 mesh sieves, mix with sugar or amino acid the nutrient made.
In nucleic acid hydrolysis process described in this preparation method, alkali lye used is sodium hydroxide or potassium hydroxide solution.
In the present invention, nucleosides hydrochlorate mainly refers to: 5 '-adenylate disodium or potassium, 5 '-bird adenylate disodium or potassium, 5 '-cytidylic acid disodium or potassium, the sodium salt of 5 '-uridine monophosphate disodium or potassium four kinds of 5 '-mixed nucleotides or sylvite; Deoxyribonucleoside hydrochlorate refers to: 5 '-desoxyadenossine acid disodium or potassium, 5 '-deoxidation bird adenylate disodium or potassium, 5 '-deoxycytidine acid disodium or potassium, 5 '-BrdU acid disodium,, the sodium salt of 5 '-deoxyribosylthymine acid disodium or potassium five kinds of 5 '-deoxidation mixed nucleotides or sylvite.
The source of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate is take nucleic acid as raw material, mainly the nucleic acid of dry method or wet production is adopted to be raw material from the mycelium after fermentation plant fermentation, this kind of nucleic acid is a small amount of DNA (deoxyribonucleic acid) (DNA) of main mixing with ribonucleic acid (RNA), makes with alkali lye hydrolysis; Auxiliary agent is mainly derived from two aspects: 1, the nucleic acid of dry method or wet production is also containing impurity such as sugar, protein, various metals or nonmetallic ions, has (impurity) such as amino acid, sugared part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese after hydrolysis; 2, exogenously to add: sugar (reducing sugar, non reducing sugar: as glucose, sucrose also have fructose etc.), amino acid, large, middle and trace element etc.
The present invention adopt primarily of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, auxiliary agent composition Promoting plant growth nutrient and be take nucleic acid as raw material, mainly from fermentation plant (Gourmet Powder Factory, brewery, antibiotics plant, yeast factory, Amino Acid Factory, paper mill, activated sludge after waste water treatment, petro-jelly fermentation etc.) by product (offcuts) mycelium after fermentation adopts the nucleic acid of dry method or wet production to be the preparation method of raw material hydrolysis, (product of Ribavirin is added with existing product and method, directly with the product of ribonucleic acid hydrolysis, enzyme solution) compare and have the following advantages: 1, its production cost of ribonucleic acid is produced low from the mycelium after fermentation plant fermentation, be easy to large-scale production and popularization, 2, the nucleic acid that mycelium is produced contains useful impurity, in addition Base hydrolysis method, technique is simple, mixed system nucleotide salt yield is high, and rationally, amino acid, sugared part and other organic-inorganic auxiliary agent content are higher for various nucleosides hydrochlorate and the mutual mixing ratio of deoxynucleotide salt face, therefore, increasing both production and income synergy, strengthens resistance, improves quality effective, 3, nontoxic, harmless, noresidue, without any adverse side effect, 4, be widely used in grain, cotton, oil, cigarette, tea, really, dish, traditional Chinese medicine, flowers, plant and the mushroom product such as forest.
Embodiment
Embodiment 1: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 1 gram, glucose 6 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix, obtain nutrient with glucose.
Embodiment 2: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 1 gram, sucrose 2 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix, obtain nutrient with glucose.
Embodiment 3: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 2 grams, glucose 8 grams, nucleosides hydrochlorate is with de-
Oxygen nucleosides hydrochlorate is pulverized, and mixes, obtain nutrient with glucose.
Embodiment 4: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 1.5 grams, glucose 8.5 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix, obtain nutrient with glucose.
Embodiment 5: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 1 gram, glucose 9 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix, obtain nutrient with glucose.
Embodiment 6: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 2.5 grams, glucose 7.5 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix, obtain nutrient with glucose.
Embodiment 7: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 4 grams, 6 grams, fructose, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix, obtain nutrient with glucose.
Embodiment 8: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 5 grams, glucose 5 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix, obtain nutrient with glucose.
Embodiment 9: get nucleotide potassium and 1 gram, deoxynucleotide potassium, glucose 9 grams, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix, obtain nutrient with glucose.
Embodiment 10: get nucleotide potassium and 1.5 grams, deoxynucleotide potassium, glucose 8.5 grams, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix, obtain nutrient with glucose.
Embodiment 11: get nucleotide potassium and 2 grams, deoxynucleotide potassium, glucose 8 grams, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix, obtain nutrient with glucose.
Embodiment 12: get nucleotide potassium and 2.5 grams, deoxynucleotide potassium, glucose 7.5 grams, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix, obtain nutrient with glucose.
Embodiment 13: get nucleotide potassium and 3 grams, deoxynucleotide potassium, glucose 7 grams, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix, obtain nutrient with glucose.
Embodiment 14: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 1.5 grams, glucose 4.5 grams, 4 grams, amino acid, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix, obtain nutrient with glucose.
Embodiment 15: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 2 grams, glucose 4 grams, 4 grams, amino acid, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix, obtain nutrient with glucose.
Embodiment 16: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 3 grams, glucose 4 grams, 3 grams, amino acid, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix, obtain nutrient with glucose.
Embodiment 17: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 2 grams, 8 grams, amino acid, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix, obtain nutrient.
Embodiment 18: by 100 grams, the crude product ribonucleic acid (RNA) obtained from the mycelium of fermentation plant's by product by dry method or wet method, be dissolved in the sodium hydroxide solution of 3 liters of 6.3N, under 37 DEG C of conditions, stir 12 hours, obtain and mainly refer to contain: 5 '-adenylate disodium, 5 '-bird adenylate disodium, 5 '-cytidylic acid disodium, the sodium salt of 5 '-uridine monophosphate disodium, four kinds of 5 '-mixed nucleotides also has deoxyribonucleoside hydrochlorate: 5 '-desoxyadenossine acid disodium, 5 '-deoxidation bird adenylate disodium, 5 '-deoxycytidine acid disodium, 5 '-BrdU acid disodium, , the sodium salt of 5 '-deoxyribosylthymine acid disodium, five kinds of 5 '-deoxidation mixed nucleotides and impurity (amino acid, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc.) solution, filter, filtrate is neutralized to pH8-8.5, be evaporated to appropriate, concentrate is added activated carbon decolorizing, suction filtration it, filtrate reduced in volume is to moisture 15%, nearly dry new-nucleo and deoxyribonucleotide natrium is dissolved by proper amount of clear water, its solution adds alcohol and separates out, crystallization suction filtration is dry, about obtain 85-90 grams, white core thuja acid salt and deoxyribonucleoside hydrochlorate and a certain amount ofly to help and agent water soluble mixt, gains are pulverized, crosses 200 mesh sieves, mix with auxiliary agent.
Embodiment 19: by 100 grams, the crude product ribonucleic acid (RNA) obtained from the mycelium of fermentation plant's by product by dry method or wet method, be dissolved in the potassium hydroxide solution of 2.5 liter of 6 N, under 40 DEG C of conditions, stir 10 hours, obtain and mainly refer to contain: 5 '-adenylate dipotassium, 5 '-bird adenylate dipotassium, 5 '-cytidylic acid dipotassium, the sylvite of 5 '-uridylic acid dipotassium, four kinds of 5 '-mixed nucleotides also has deoxyribonucleoside hydrochlorate: 5 '-deoxyadenylic acid dipotassium, 5 '-deoxidation bird adenylate dipotassium, 5 '-deoxycytidylic acid dipotassium, 5 '-
Deoxyuridylic acid dipotassium,, the sylvite of 5 '-deoxythymidylic acid dipotassium, five kinds of 5 '-deoxidation mixed nucleotides and amino acid, sugared part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc.) and solution, filters, filtrate is neutralized to pH8-8.5, is evaporated to appropriate; Concentrate is added activated carbon decolorizing, suction filtration it, filtrate reduced in volume to 20%; Dissolve near dry nucleotide potassium and deoxynucleotide potassium by proper amount of clear water, its solution adds alcohol and separates out, and crystallization suction filtration is dry, about 88-93 grams of white core thuja acid sylvite and deoxyribonucleoside acid potassium salt and a certain amount of water soluble mixt (impurity).Gains are pulverized, crosses 200 mesh sieves, mix with auxiliary agent.
Embodiment 20: paddy rice: spray twice, first time is watered 30 liters with 20 grams of nutrients (containing glucose 80-85%'s), about concentration 100ppm for initial bloom stage (heading about 10%), evenly sprays on one mu of paddy rice.Second time is full-bloom stage, after first time sprays, within 5-6 days, be watered 30 liters with 20 grams of nutrients, evenly sprays and gets final product on one mu of paddy rice (mu volume increase 70-100 kilograms, volume increase about 15%).
Embodiment 21: cotton: spray twice, first time also can initial bloom stage in the flower bud phase, is watered 30 liters, evenly sprays on one mu of cotton with 30 grams of nutrients.Second time full-bloom stage is watered 36 liters with 30 grams, and evenly spray on one mu of cotton can (mu increases production more than 7%).
Embodiment 22: rape: spray twice, first time is initial bloom stage, is watered 20 liters sprays on one mu of rape with 20 grams of nutrients.Second time is full-bloom stage, is watered 30 liters, sprays on one mu of rape with 20 grams, volume increase 15-20 kilogram.
Embodiment 23: tobacco: spray twice, each use 30 grams, is often watered 30 kilograms, about concentration 200ppm, spray on one mu of tobacco leaf, spray and carry out 20-26 day in time in May, (the south north time is slightly different), first time sprays, 3-6 day in June carry out second time to spray, about concentration 150ppm, Quality advance grade, volume increase about 20%.
Embodiment 24: tealeaves: at growth germination period, spray first time, after often plucking, spray once, about concentration 180ppm.
Embodiment 25: capsicum: each once at initial bloom stage and full-bloom stage, concentration 200-300ppm, mu volume increase 40% ~ 80%.
Embodiment 26: strawberry: spray in the fruitlet phase, be watered 18 liters with 10 grams and spray, spraying 3 ~ 4 times after harvesting can increase production 38% in batches.
Embodiment 27: traditional Chinese medicine: the traditional Chinese medicine of different parts, using method differs, generally each once at initial bloom stage and full-bloom stage, concentration 100-260ppm.
Embodiment 28: mushroom: spray once in every 3 days from fruiting, concentration 100-150ppm, volume increase 50-80%, shortens collecting time 1/3rd.
Claims (4)
1. the implantation methods of strawberry, is characterized in that: at strawberry growing period, use nutrient; Described nutrient is primarily of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, sugar or amino acid composition, its percentage by weight is: nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 10-30%, sugar or amino acid 70-90%, the nucleosides hydrochlorate in described nutrient and deoxyribonucleoside hydrochlorate be with fermentation plant fermentation after mycelium adopt the nucleic acid of dry method or wet production for raw material:
1., the hydrolysis of nucleic acid is the ratio mixed nucleus bronsted lowry acids and bases bronsted lowry liquid of 1:20-30 according to nucleic acid, buck weight ratio, and alkali lye is 5.5-7N, under 30-45 DEG C of condition, stir 10-14 hours, obtain nucleosides hydrochlorate and deoxyribonucleoside acid salt solution, filter, filtrate adjusts pH8-8.5, reduced pressure concentration;
2., decolouring, dry concentrate is added activated carbon decolorizing, suction filtration, filtrate reduced in volume is to moisture 10-25%;
3., crystallization with clear water dissolve dry after nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, its solution adds alcohol and separates out, and crystallization suction filtration is dry, the white core thuja acid salt obtained and deoxyribonucleoside hydrochlorate and water soluble mixt;
4., by nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and water soluble mixt pulverize, cross 200 mesh sieves and mix with sugar or amino acid again;
Sugar in described nutrient is glucose;
The time that described growing period uses nutrient is: spray once in the fruitlet phase, sprays 3 ~ 4 times after harvesting in batches.
2. according to the implantation methods of strawberry described in claim 1, it is characterized in that: the consumption using nutrient is: be watered 18 liters with 10 grams and spray.
3. according to the implantation methods of strawberry described in claim 1, it is characterized in that: nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are nucleotide potassium or sodium salt and deoxynucleotide potassium or sodium salt.
4. according to the implantation methods of strawberry described in claim 1, it is characterized in that: in the nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate of described nutrient, percentage by weight is: nucleosides hydrochlorate 85-99%, deoxyribonucleoside hydrochlorate 1-15%.
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| CN1107454A (en) * | 1994-11-05 | 1995-08-30 | 岳仁芳 | Organic fertilizer composition for spray application |
| CN1142896A (en) * | 1996-07-18 | 1997-02-19 | 师昌友 | Regulating agent for plant growth and its preparing method |
| CN1276360A (en) * | 1999-08-12 | 2000-12-13 | 河南省科学院生物研究所 | Foliage fertilizer |
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