CN103348843A - Planting method of strawberries with good quality and high yield - Google Patents
Planting method of strawberries with good quality and high yield Download PDFInfo
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- CN103348843A CN103348843A CN2013102495606A CN201310249560A CN103348843A CN 103348843 A CN103348843 A CN 103348843A CN 2013102495606 A CN2013102495606 A CN 2013102495606A CN 201310249560 A CN201310249560 A CN 201310249560A CN 103348843 A CN103348843 A CN 103348843A
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- hydrochlorate
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- potassium
- glucose
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- 238000000034 method Methods 0.000 title claims abstract description 49
- 235000021012 strawberries Nutrition 0.000 title claims abstract 5
- 240000009088 Fragaria x ananassa Species 0.000 title 1
- 235000015097 nutrients Nutrition 0.000 claims abstract description 69
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 47
- 150000001413 amino acids Chemical class 0.000 claims abstract description 28
- 241000220223 Fragaria Species 0.000 claims abstract description 26
- 235000016623 Fragaria vesca Nutrition 0.000 claims abstract description 22
- 235000011363 Fragaria x ananassa Nutrition 0.000 claims abstract description 22
- 229910052700 potassium Inorganic materials 0.000 claims description 72
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 71
- 239000011591 potassium Substances 0.000 claims description 71
- 239000002777 nucleoside Substances 0.000 claims description 70
- 125000003835 nucleoside group Chemical group 0.000 claims description 69
- 239000005549 deoxyribonucleoside Substances 0.000 claims description 68
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 55
- 239000008103 glucose Substances 0.000 claims description 55
- 239000002773 nucleotide Substances 0.000 claims description 42
- 102000039446 nucleic acids Human genes 0.000 claims description 32
- 108020004707 nucleic acids Proteins 0.000 claims description 32
- 150000007523 nucleic acids Chemical class 0.000 claims description 32
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 claims description 31
- 239000000203 mixture Substances 0.000 claims description 30
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 24
- 239000007921 spray Substances 0.000 claims description 23
- 238000002513 implantation Methods 0.000 claims description 22
- 241000196324 Embryophyta Species 0.000 claims description 20
- 239000002253 acid Substances 0.000 claims description 20
- 238000004519 manufacturing process Methods 0.000 claims description 20
- 238000000855 fermentation Methods 0.000 claims description 17
- 230000004151 fermentation Effects 0.000 claims description 17
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 claims description 15
- 239000000243 solution Substances 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 14
- 239000000706 filtrate Substances 0.000 claims description 14
- 238000000967 suction filtration Methods 0.000 claims description 14
- 229920002477 rna polymer Polymers 0.000 claims description 13
- 239000012752 auxiliary agent Substances 0.000 claims description 11
- 239000012535 impurity Substances 0.000 claims description 11
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 10
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 10
- 238000002425 crystallisation Methods 0.000 claims description 10
- 230000008025 crystallization Effects 0.000 claims description 10
- 239000002994 raw material Substances 0.000 claims description 9
- 230000007062 hydrolysis Effects 0.000 claims description 8
- 238000006460 hydrolysis reaction Methods 0.000 claims description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 7
- 239000012141 concentrate Substances 0.000 claims description 7
- 230000006837 decompression Effects 0.000 claims description 7
- 229910052698 phosphorus Inorganic materials 0.000 claims description 7
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 6
- 239000003513 alkali Substances 0.000 claims description 6
- -1 filter Substances 0.000 claims description 6
- 239000011574 phosphorus Substances 0.000 claims description 6
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 5
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 5
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 5
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims description 5
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 5
- 239000006227 byproduct Substances 0.000 claims description 5
- 229910052791 calcium Inorganic materials 0.000 claims description 5
- 239000011575 calcium Substances 0.000 claims description 5
- 229910052802 copper Inorganic materials 0.000 claims description 5
- 239000010949 copper Substances 0.000 claims description 5
- 229910052742 iron Inorganic materials 0.000 claims description 5
- 239000011777 magnesium Substances 0.000 claims description 5
- 229910052749 magnesium Inorganic materials 0.000 claims description 5
- 229910052748 manganese Inorganic materials 0.000 claims description 5
- 239000011572 manganese Substances 0.000 claims description 5
- 229910052757 nitrogen Inorganic materials 0.000 claims description 5
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- 239000011701 zinc Substances 0.000 claims description 5
- 229910052725 zinc Inorganic materials 0.000 claims description 5
- WOVKYSAHUYNSMH-RRKCRQDMSA-N 5-bromodeoxyuridine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(Br)=C1 WOVKYSAHUYNSMH-RRKCRQDMSA-N 0.000 claims description 4
- 229930183912 Cytidylic acid Natural products 0.000 claims description 4
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- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 4
- 229930006000 Sucrose Natural products 0.000 claims description 4
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 claims description 4
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- IERHLVCPSMICTF-UHFFFAOYSA-N cytidine monophosphate Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(COP(O)(O)=O)O1 IERHLVCPSMICTF-UHFFFAOYSA-N 0.000 claims description 4
- KURVIXMFFSNONZ-WFIJOQBCSA-L disodium;[(2r,3s,4r,5r)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound [Na+].[Na+].O[C@@H]1[C@H](O)[C@@H](COP([O-])([O-])=O)O[C@H]1N1C(=O)NC(=O)C=C1 KURVIXMFFSNONZ-WFIJOQBCSA-L 0.000 claims description 4
- 238000003306 harvesting Methods 0.000 claims description 4
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- CKTSBUTUHBMZGZ-SHYZEUOFSA-N 2'‐deoxycytidine Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 CKTSBUTUHBMZGZ-SHYZEUOFSA-N 0.000 claims description 3
- CKTSBUTUHBMZGZ-UHFFFAOYSA-N Deoxycytidine Natural products O=C1N=C(N)C=CN1C1OC(CO)C(O)C1 CKTSBUTUHBMZGZ-UHFFFAOYSA-N 0.000 claims description 3
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 claims description 3
- 239000002585 base Substances 0.000 claims description 3
- 150000007516 brønsted-lowry acids Chemical class 0.000 claims description 3
- 150000007528 brønsted-lowry bases Chemical class 0.000 claims description 3
- 239000005547 deoxyribonucleotide Substances 0.000 claims description 3
- 125000002637 deoxyribonucleotide group Chemical group 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 239000012266 salt solution Substances 0.000 claims description 3
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 2
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 8
- 230000012010 growth Effects 0.000 abstract description 7
- 150000003839 salts Chemical class 0.000 abstract description 5
- 230000002411 adverse Effects 0.000 abstract description 3
- 230000001965 increasing effect Effects 0.000 abstract description 3
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 3
- 231100000252 nontoxic Toxicity 0.000 abstract description 3
- 230000003000 nontoxic effect Effects 0.000 abstract description 3
- KCIDZIIHRGYJAE-YGFYJFDDSA-L dipotassium;[(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] phosphate Chemical compound [K+].[K+].OC[C@H]1O[C@H](OP([O-])([O-])=O)[C@H](O)[C@@H](O)[C@H]1O KCIDZIIHRGYJAE-YGFYJFDDSA-L 0.000 description 9
- 230000008635 plant growth Effects 0.000 description 9
- 230000001737 promoting effect Effects 0.000 description 7
- 239000003337 fertilizer Substances 0.000 description 6
- 239000001103 potassium chloride Substances 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 239000000047 product Substances 0.000 description 6
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- 229920000742 Cotton Polymers 0.000 description 4
- 240000007594 Oryza sativa Species 0.000 description 4
- 235000007164 Oryza sativa Nutrition 0.000 description 4
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- IWUCXVSUMQZMFG-AFCXAGJDSA-N Ribavirin Chemical compound N1=C(C(=O)N)N=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](CO)O1 IWUCXVSUMQZMFG-AFCXAGJDSA-N 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
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- 229960000329 ribavirin Drugs 0.000 description 4
- HZCAHMRRMINHDJ-DBRKOABJSA-N ribavirin Natural products O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1N=CN=C1 HZCAHMRRMINHDJ-DBRKOABJSA-N 0.000 description 4
- 235000009566 rice Nutrition 0.000 description 4
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 3
- 239000007857 degradation product Substances 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
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- 235000001674 Agaricus brunnescens Nutrition 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 2
- 244000061176 Nicotiana tabacum Species 0.000 description 2
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- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 2
- KHWCHTKSEGGWEX-RRKCRQDMSA-N 2'-deoxyadenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(O)=O)O1 KHWCHTKSEGGWEX-RRKCRQDMSA-N 0.000 description 1
- NCMVOABPESMRCP-SHYZEUOFSA-N 2'-deoxycytosine 5'-monophosphate Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)C1 NCMVOABPESMRCP-SHYZEUOFSA-N 0.000 description 1
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- KHWCHTKSEGGWEX-UHFFFAOYSA-N deoxyadenylic acid Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(O)=O)O1 KHWCHTKSEGGWEX-UHFFFAOYSA-N 0.000 description 1
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Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Saccharide Compounds (AREA)
Abstract
The invention discloses a planting method of strawberries with good quality and high yield. The method comprises the steps as follows: nutrient is applied during the strawberry growth period; the nutrient mainly comprises components in percentage by weight as follows: 10%-80% of nucleotide salt and deoxynucleotide salt and 20%-90% of sugar or/ and amino acid; and the time of applying the nutrient during the growth period is that the nutrient is sprayed once during the fruitlet period, and sprayed for 3-4 times in batches after picking is finished. The planting method has the advantages that the nutrient is non-toxic and harmless, and has no residue and no adverse side effect; yield, income and efficiency are increased, resistance is enhanced, and an effect of improving quality is good; and further, the method is simple, low in cost and prone to large-scale production and popularization.
Description
The application is application number 201010514217.6, the applying date: on October 21st, 2010, denomination of invention is divided an application for " nutrient of a kind of Promoting plant growth and preparation method thereof ".
Technical field
The present invention relates to the good quality and high output implantation methods of a kind of implantation methods of berry, particularly a kind of strawberry.
Background technology
Greatly develop plant husbandry, forestry, promote that crop yield increases income, make our country green, it is the problem that the various countries scientist pays close attention to research, the growth of plant be unable to do without to supplement the nutrients, and present plant husbandry is mainly by executing N,P,K fertilizers and organic manure promotes crop growth, use too much fertilizer very large on the quality and flavor impact of crops, be unfavorable for development and food security and the environmental protection of green agriculture.Therefore, people have studied growth hormone relevant to nucleic acid, nutrient, fertilizer.In theory: nucleic acid is the basic substance that consists of life, is the basis of metabolism and energy metabolism, and the character of nucleic acid and the difference of quantity can cause the diversity of biological species and growth conditions.For congener life entity, the quantity of nucleic acid and the difference of existence form have determined strong and weak prosperous the declining of this life entity.Therefore, nucleic acid substances has irreplaceable effect for the output increased in crops and even the production of whole vegetative kingdom and product quality improvement; Particularly several nucleotides salt and deoxyribonucleoside hydrochlorate and the cooperations thereof of the little molecule of nucleic acid such as nucleosides, nucleotide, pyrimidine and purine, the not only very effectively growth of regulating plant, can be used as again nutrient source is absorbed rapidly by plant, strengthen the plant root vigor, improve the plant anti-adversity ability, thus improve crop output, improve product quality.The little molecule of nucleic acid has the enhancing improving activity of root system to plant, improves root to the absorption of phosphorus, potash fertilizer; Improve the activity of crops malate dehydrogenase and succinic acid enzyme, promote the assimilational capacity of plant, strengthen photosynthetic intensity, to plant growth have regulate and trophism (Wang Xi etc. the application and function research on mechanism [J] of nucleic acid degradation product on Rice Production. Acta Biochimica et Biophysica Sinica, 1975,7 (1): 31-39).3., the hydrolysis of nucleic acid 2., dissolving ribonucleic acid (alkali lye hydrotropy) 1., the lixiviate nuclease (publication number: 101186534) disclose a kind of preparation method who contains the nucleic acid degradation product foliage fertilizer, the concrete step is Chinese patent literature:,,, must contain
The little molecule of multiple nucleic acids and a small amount of reducing sugar and few big or middle, trace elements such as nucleosides, nucleotide, purine, pyrimidine are arranged, the method is take ribonucleic acid as raw material, with enzymatic hydrolysis, exist production cost high, degrade not thorough, seldom contain the deoxynucleotide composition, nucleotide salt yield is low in the mixed system, the mutual mixing ratio imbalance of various nucleosides hydrochlorates and deoxynucleotide salt face is very large on the result of use impact.Chinese patent literature (publication number: 101050143) disclose a kind of nutrient that promotes crop growth, it is comprised of Ribavirin and glucose, production and the guanosine of Ribavirin are in close relations, and there is the Ribavirin high cost in this patent, the unconspicuous problem of practical effect; Chinese patent literature (publication number: 1238323) disclose a kind of preparation method of nucleic acid foliage fertilizer, the method of alkaline hydrolysis is adopted in the degraded of its nucleic acid, degradation product is purine and pyrimidine, there is the nucleic acid high cost equally in this patent, various nucleosides hydrochlorates and the mutual mixing ratio of deoxynucleotide salt face are unreasonable, affect the problem of result of use.
Summary of the invention
The purpose of this invention is to provide the good quality and high output implantation methods of a kind of strawberry, its production cost is low, and method is simple, is easy to large-scale production and popularization; Nucleotide salt yield is high in the mixed system, and various nucleosides hydrochlorates and the mutual mixing ratio of deoxynucleotide salt face are reasonable, nontoxic, harmless, noresidue, without any adverse side effect; Increase production, increase income, synergy, strengthen resistance, improve quality effective;
The present invention is achieved in that
The good quality and high output implantation methods of strawberry: at the strawberry growing period, use nutrient; Described nutrient mainly by nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, sugar or/and amino acid form, its percentage by weight is: nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 10-80%, sugar is or/and amino acid 20-90%, the time that described growing period is used nutrient is: spray once in the fruitlet phase, spray after the harvesting 3～4 times in batches.
The good quality and high output implantation methods of strawberry: the consumption of using nutrient is: be watered 18 liters with 10 grams and spray
The good quality and high output implantation methods of strawberry: the nucleosides hydrochlorate in the described nutrient and deoxyribonucleoside hydrochlorate, sugar are or/and amino acid, and its percentage by weight is: 10-30%, and auxiliary agent 70-90%.
The good quality and high output implantation methods of strawberry: nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are nucleotide potassium or sodium salt and deoxynucleotide potassium or sodium salt.
The good quality and high output implantation methods of strawberry: the sugar in the described nutrient is glucose or sucrose or fructose.
The good quality and high output implantation methods of strawberry is in the nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate of described nutrient, and percentage by weight is: nucleosides hydrochlorate 85-99%, deoxyribonucleoside hydrochlorate 1-15%.
The good quality and high output implantation methods of strawberry: the nucleosides hydrochlorate in the described nutrient and deoxyribonucleoside hydrochlorate be the mycelium after the fermentation plant fermentation adopt dry method or wet production nucleic acid as raw material:
1., the hydrolysis of nucleic acid is the ratio mixed nucleus bronsted lowry acids and bases bronsted lowry liquid of 1:20-30 according to nucleic acid, buck weight ratio, alkali lye is 5.5-7N, under 30-45 ℃ of condition, stirred 10-14 hours, and got nucleosides hydrochlorate and deoxyribonucleoside acid salt solution, filter, filtrate is transferred pH8-8.5, reduced pressure concentration;
2., decolouring, dry concentrate is added activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 10-25%;
3., nucleosides hydrochlorate and the deoxyribonucleoside hydrochlorate of crystallization after doing with clear water dissolving, its solution adds alcohol separates out, and the crystallization suction filtration is dry, the white nucleosides hydrochlorate that obtains and deoxyribonucleoside hydrochlorate and water soluble mixt (impurity).
The good quality and high output implantation methods of strawberry: described nutrient is crude product ribonucleic acid 100 grams that make from the mycelium of fermentation plant's by product with dry method or wet method, be dissolved in the sodium hydroxide solution of 3 liters of 6.3N, under 37 ℃ of conditions, stirred 12 hours, the winner will refer to contain: 5 '-the adenylate disodium, 5 '-bird adenylate disodium, 5 '-the cytidylic acid disodium, 5 '-four kind 5 of uridine monophosphate disodium '-sodium salt of mixed nucleotides also has the deoxyribonucleoside hydrochlorate: 5 '-the desoxyadenossine acid disodium, 5 '-deoxidation bird adenylate disodium, 5 '-the deoxycytidine acid disodium, 5 '-the BrdU acid disodium, 5 '-five kind 5 of deoxyribosylthymine acid disodium '-sodium salt and the impurity (amino acid of deoxidation mixed nucleotides, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc.) solution, filter, filtrate is neutralized to pH8-8.5, is evaporated to an amount of; Concentrate is added activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 15%; With nearly new-nucleo and the deoxyribonucleotide natrium of doing of proper amount of clear water dissolving, its solution adds alcohol and separates out, the crystallization suction filtration is dry, approximately get 85-90 grams, white nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and a certain amount of water soluble mixt (impurity), gains are pulverized, are crossed 200 mesh sieves, with sugar or/and amino acid mixes the nutrient of evenly making.
The good quality and high output implantation methods of strawberry is characterized in that: described nutrient refers to: get nucleotide potassium and deoxynucleotide potassium 1.5 grams, and glucose 8.5 grams, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix the nutrient made from glucose; Perhaps refer to:
Get nucleotide potassium and deoxynucleotide potassium 2 grams, glucose 8 grams, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix the nutrient made from glucose; Perhaps refer to: get nucleotide potassium and deoxynucleotide potassium 2.5 grams, glucose 7.5 grams, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix the nutrient made from glucose; Perhaps refer to: get nucleotide potassium and deoxynucleotide potassium 3 grams, glucose 7 grams, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix the nutrient made from glucose; Perhaps refer to: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 1.5 grams, glucose 4.5 grams, amino acid 4 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix the nutrient made from glucose.
The nutrient of Promoting plant growth, in nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, percentage by weight is: nucleosides hydrochlorate 88-96%, deoxyribonucleoside hydrochlorate 4-12%.
The nutrient of Promoting plant growth, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and auxiliary agent, percentage by weight is:
Nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 10-30%, auxiliary agent 70-90%.
The nutrient of Promoting plant growth, auxiliary agent are sugar or amino acid.
The nutrient of Promoting plant growth, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are sodium salt or sylvite.
The preparation method of the nutrient of Promoting plant growth provided by the present invention, concrete steps are as follows: take nucleic acid as raw material, mainly be that to adopt the nucleic acid of dry method or wet production be raw material for mycelium after the fermentation plant fermentation:
1., the hydrolysis of nucleic acid is the ratio mixed nucleus bronsted lowry acids and bases bronsted lowry liquid of 1:20-30 according to nucleic acid, buck weight ratio, alkali lye is 5.5-7N, under 30-45 ℃ of condition, stirred 10-14 hours, and got nucleosides hydrochlorate and deoxyribonucleoside acid salt solution, filter, filtrate is transferred pH8-8.5, reduced pressure concentration;
2., decolouring, dry concentrate is added activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 10-25%;
3., nucleosides hydrochlorate and the deoxyribonucleoside hydrochlorate of crystallization after doing with clear water dissolving, its solution adds alcohol separates out, and the crystallization suction filtration is dry, gets white nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and water soluble mixt.
4., nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and water soluble mixt are pulverized, cross 200 mesh sieves and mix with auxiliary agent again.
In the described nucleic acid hydrolysis process of this preparation method, used alkali lye is sodium hydroxide or potassium hydroxide solution.
In the present invention, the nucleosides hydrochlorate mainly refers to: 5 '-adenylate disodium or potassium, 5 '-bird adenylate disodium or potassium, 5 '-cytidylic acid disodium or potassium, 5 '-four kind 5 in uridine monophosphate disodium or potassium '-sodium salt or the sylvite of mixed nucleotides; The deoxyribonucleoside hydrochlorate refers to: 5 '-desoxyadenossine acid disodium or potassium, 5 '-deoxidation bird adenylate disodium or potassium, 5 '-deoxycytidine acid disodium or potassium, 5 '-the BrdU acid disodium,, 5 '-five kind 5 in deoxyribosylthymine acid disodium or potassium '-sodium salt or the sylvite of deoxidation mixed nucleotides.The source of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate is for take nucleic acid as raw material, mainly be mycelium after the fermentation plant fermentation to adopt the nucleic acid of dry method or wet production be raw material, this kind nucleic acid mixes a small amount of deoxidation nuclear take ribonucleic acid (RNA) as main
Ribosomal ribonucleic acid (DNA) is made with the alkali lye hydrolysis; Auxiliary agent is mainly derived from two aspects: 1, the nucleic acid of dry method or wet production also contains the impurity such as sugar, protein, various metals or nonmetallic ion, and (impurity) such as amino acid, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese is arranged after the hydrolysis; 2, exogenous adding: sugar (reducing sugar, non reducing sugar: also have fructose etc. such as glucose, sucrose), amino acid, big or middle, micro-etc.
The present invention adopt mainly by nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, it is take nucleic acid as raw material that the Promoting plant growth nutrient that auxiliary agent forms reaches, mainly be from fermentation plant (Gourmet Powder Factory, brewery, antibiotics plant, yeast factory, Amino Acid Factory, the paper mill, activated sludge after the waste water treatment, petro-jelly fermentations etc.) nucleic acid of the employing dry method of by product (offcuts) mycelium after the fermentation or wet production is the preparation method of raw material hydrolysis, with existing product and the method (product that adds Ribavirin, the product that directly is hydrolyzed with ribonucleic acid, enzyme solution) compares and have the following advantages: 1, its production cost of mycelium production ribonucleic acid after the fermentation plant fermentation is low, is easy to large-scale production and popularization; 2, the nucleic acid of mycelium production contains useful impurity, in addition basic hydrolysis method, technique is simple, nucleotide salt yield is high in the mixed system, and various nucleosides hydrochlorates and the mutual mixing ratio of deoxynucleotide salt face are reasonable, and amino acid, sugar part and other organic-inorganic auxiliary agent content are higher, therefore, increasing both production and income synergy strengthens resistance, improves quality effective; 3,, nontoxic, harmless, noresidue, without any adverse side effect; 4, be widely used in grain, cotton, oil, cigarette, tea, really, plant and the bacterium mushroom series products such as dish, traditional Chinese medicine, flowers, forest.
Embodiment
Embodiment 1: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 1 gram, and glucose 6 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, namely get nutrient.
Embodiment 2: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 1 gram, and sucrose 2 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, namely get nutrient.
Embodiment 3: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 2 grams, glucose 8 grams, nucleosides hydrochlorate and taking off
Oxygen nucleosides hydrochlorate is pulverized, and mixes with glucose, namely gets nutrient.
Embodiment 4: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 1.5 grams, and glucose 8.5 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, namely get nutrient.
Embodiment 5: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 1 gram, and glucose 9 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, namely get nutrient.
Embodiment 6: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 2.5 grams, and glucose 7.5 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, namely get nutrient.
Embodiment 7: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 4 grams, and fructose 6 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, namely get nutrient.
Embodiment 8: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 5 grams, and glucose 5 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, namely get nutrient.
Embodiment 9: get nucleotide potassium and deoxynucleotide potassium 1 gram, and glucose 9 grams, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, namely get nutrient.
Embodiment 10: get nucleotide potassium and deoxynucleotide potassium 1.5 grams, and glucose 8.5 grams, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, namely get nutrient.
Embodiment 11: get nucleotide potassium and deoxynucleotide potassium 2 grams, and glucose 8 grams, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, namely get nutrient.
Embodiment 12: get nucleotide potassium and deoxynucleotide potassium 2.5 grams, and glucose 7.5 grams, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, namely get nutrient.
Embodiment 13: get nucleotide potassium and deoxynucleotide potassium 3 grams, and glucose 7 grams, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, namely get nutrient.
Embodiment 14: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 1.5 grams, and glucose 4.5 grams, amino acid 4 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix with glucose, namely get nutrient.
Embodiment 15: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 2 grams, and glucose 4 grams, amino acid 4 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix with glucose, namely get nutrient.
Embodiment 16: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 3 grams, and glucose 4 grams, amino acid 3 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix with glucose, namely get nutrient.
Embodiment 17: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 2 grams, and amino acid 8 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix, and namely get nutrient.
Embodiment 18: crude product ribonucleic acid (RNA) 100 grams that will make from the mycelium of fermentation plant's by product with dry method or wet method, be dissolved in the sodium hydroxide solution of 3 liters of 6.3N, under 37 ℃ of conditions, stirred 12 hours, the winner will refer to contain: 5 '-the adenylate disodium, 5 '-bird adenylate disodium, 5 '-the cytidylic acid disodium, 5 '-four kind 5 of uridine monophosphate disodium '-sodium salt of mixed nucleotides also has the deoxyribonucleoside hydrochlorate: 5 '-the desoxyadenossine acid disodium, 5 '-deoxidation bird adenylate disodium, 5 '-the deoxycytidine acid disodium, 5 '-the BrdU acid disodium, 5 '-five kind 5 of deoxyribosylthymine acid disodium '-sodium salt and the impurity (amino acid of deoxidation mixed nucleotides, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc.) solution, filter, filtrate is neutralized to pH8-8.5, is evaporated to an amount of; Concentrate is added activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 15%; With nearly new-nucleo and the deoxyribonucleotide natrium of doing of proper amount of clear water dissolving, its solution adds alcohol and separates out, the crystallization suction filtration is dry, approximately get 85-90 grams, white nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and a certain amount of helping and the agent water soluble mixt, gains are pulverized, crossed 200 mesh sieves, mix with auxiliary agent.
Embodiment 19: crude product ribonucleic acid (RNA) 100 grams that will make from the mycelium of fermentation plant's by product with dry method or wet method, be dissolved in the potassium hydroxide solution of 2.5 liter of 6 N, under 40 ℃ of conditions, stirred 10 hours, the winner will refer to contain: 5 '-the adenylate dipotassium, 5 '-bird adenylate dipotassium, 5 '-the cytidylic acid dipotassium, 5 '-four kind 5 of uridylic acid dipotassium '-sylvite of mixed nucleotides also has the deoxyribonucleoside hydrochlorate: 5 '-the deoxyadenylic acid dipotassium, 5 '-deoxidation bird adenylate dipotassium, 5 '-the deoxycytidylic acid dipotassium, 5 '-
The deoxyuridylic acid dipotassium,, 5 '-five kind 5 of deoxythymidylic acid dipotassium '-sylvite of deoxidation mixed nucleotides and amino acid, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc.) solution, filter, filtrate is neutralized to pH8-8.5, is evaporated to an amount of; Concentrate is added activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into 20%; With nearly nucleotide potassium and the deoxynucleotide potassium of doing of proper amount of clear water dissolving, its solution adds alcohol separates out, and the crystallization suction filtration is dry, approximately gets 88-93 white nucleotide sylvite of gram and deoxyribonucleoside acid potassium salt and a certain amount of water soluble mixts (impurity).Gains are pulverized, crossed 200 mesh sieves, mix with auxiliary agent.
Embodiment 20: paddy rice: spray twice, for initial bloom stage (heading about 10%) is watered 30 liters with 20 gram nutrients (containing glucose 80-85%'s), about concentration 100ppm, evenly spray on one mu of paddy rice for the first time.Be full-bloom stage for the second time, after spraying for the first time, be watered 30 liters with 20 gram nutrients in 5-6 days, evenly spray in one mu of paddy rice and get final product (mu increase production 70-100 kilograms, increase production about 15%).
Embodiment 21: cotton: spray twice, for the first time also can initial bloom stage in the flower bud phase, and restrain nutrients with 30 and be watered 30 liters, evenly spray on one mu of cotton.For the second time full-bloom stage is watered 36 liters with 30 grams and evenly sprays in one mu of cotton and get final product (mu increase production more than 7%).
Embodiment 22: rape: spraying twice, is initial bloom stage for the first time, is watered 20 liters with 20 gram nutrients and sprays on one mu of rape.Be full-bloom stage for the second time, be watered 30 liters with 20 grams, spray on one mu of rape and get final product volume increase 15-20 kilogram.
Embodiment 23: tobacco: spray twice, with 30 grams, whenever be watered 30 kilograms, about concentration 200ppm at every turn, spray on one mu of tobacco leaf, spray and carry out 20-26 day in time in May, (the south north time is slightly different) sprays for the first time, 3-6 day in June carries out spraying the second time, about concentration 150ppm, quality improves a grade, volume increase about 20%.
Embodiment 24: tealeaves: at the growth germination period, spray for the first time, spray once after every harvesting, about concentration 180ppm.
Embodiment 25: capsicum: initial bloom stage and full-bloom stage each once, concentration 200-300ppm, mu volume increase 40%～80%.
Embodiment 26: strawberry: spray in the fruitlet phase, be watered 18 liters with 10 grams and spray, spray 3～4 times after the harvesting in batches and can increase production 38%.
Embodiment 27: traditional Chinese medicine: the traditional Chinese medicine of different parts, using method differs, generally initial bloom stage and full-bloom stage each once, concentration 100-260ppm.
Embodiment 28: mushroom: sprayed once in per 3 days from fruiting, and concentration 100-150ppm, collecting time 1/3rd is shortened in volume increase 50-80%.
Claims (9)
1. the good quality and high output implantation methods of strawberry is characterized in that: at the strawberry growing period, use nutrient; Described nutrient mainly by nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, sugar or/and amino acid form, its percentage by weight is: nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 10-80%, sugar is or/and amino acid 20-90%, the time that described growing period is used nutrient is: spray once in the fruitlet phase, spray after the harvesting 3～4 times in batches.
2. the good quality and high output implantation methods of described strawberry according to claim 1, it is characterized in that: the consumption of using nutrient is: be watered 18 liters with 10 grams and spray.
3. the good quality and high output implantation methods of described strawberry according to claim 1 is characterized in that: the nucleosides hydrochlorate in the described nutrient and deoxyribonucleoside hydrochlorate, sugar are or/and amino acid, and its percentage by weight is: 10-30%, and auxiliary agent 70-90%.
4. the good quality and high output implantation methods of described strawberry according to claim 1, it is characterized in that: nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are nucleotide potassium or sodium salt and deoxynucleotide potassium or sodium salt.
5. the good quality and high output implantation methods of described strawberry according to claim 1, it is characterized in that: the sugar in the described nutrient is glucose or sucrose or fructose.
6. the good quality and high output implantation methods of described strawberry according to claim 1, it is characterized in that: in the nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate of described nutrient, percentage by weight is: nucleosides hydrochlorate 85-99%, deoxyribonucleoside hydrochlorate 1-15%.
7. according to claim 1, the good quality and high output implantation methods of 2,3,4,5 or 6 described strawberries, it is characterized in that: the nucleosides hydrochlorate in the described nutrient and deoxyribonucleoside hydrochlorate be the mycelium after the fermentation plant fermentation adopt dry method or wet production nucleic acid as raw material:
1., the hydrolysis of nucleic acid is the ratio mixed nucleus bronsted lowry acids and bases bronsted lowry liquid of 1:20-30 according to nucleic acid, buck weight ratio, alkali lye is 5.5-7N, under 30-45 ℃ of condition, stirred 10-14 hours, and got nucleosides hydrochlorate and deoxyribonucleoside acid salt solution, filter, filtrate is transferred pH8-8.5, reduced pressure concentration;
2., decolouring, dry concentrate is added activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 10-25%;
3., nucleosides hydrochlorate and the deoxyribonucleoside hydrochlorate of crystallization after doing with clear water dissolving, its solution adds alcohol separates out, and the crystallization suction filtration is dry, the white nucleosides hydrochlorate that obtains and deoxyribonucleoside hydrochlorate and water soluble mixt (impurity).
8. according to claim 1,2,3,4, the good quality and high output implantation methods of 5 or 6 described strawberries, it is characterized in that: described nutrient is crude product ribonucleic acid 100 grams that make from the mycelium of fermentation plant's by product with dry method or wet method, be dissolved in the sodium hydroxide solution of 3 liters of 6.3N, under 37 ℃ of conditions, stirred 12 hours, the winner will refer to contain: 5 '-the adenylate disodium, 5 '-bird adenylate disodium, 5 '-the cytidylic acid disodium, 5 '-four kind 5 of uridine monophosphate disodium '-sodium salt of mixed nucleotides also has the deoxyribonucleoside hydrochlorate: 5 '-the desoxyadenossine acid disodium, 5 '-deoxidation bird adenylate disodium, 5 '-the deoxycytidine acid disodium, 5 '-the BrdU acid disodium,, 5 '-five kind 5 of deoxyribosylthymine acid disodium '-sodium salt and the impurity (amino acid of deoxidation mixed nucleotides, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc.) solution filters, filtrate is neutralized to pH8-8.5, is evaporated to an amount of; Concentrate is added activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 15%; With nearly new-nucleo and the deoxyribonucleotide natrium of doing of proper amount of clear water dissolving, its solution adds alcohol and separates out, the crystallization suction filtration is dry, approximately get 85-90 grams, white nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and a certain amount of water soluble mixt (impurity), gains are pulverized, are crossed 200 mesh sieves, with sugar or/and amino acid mixes the nutrient of evenly making.
9. according to claim 1, the good quality and high output implantation methods of 2,3,4,5 or 6 described strawberries, it is characterized in that: described nutrient refers to: get nucleotide potassium and deoxynucleotide potassium 1.5 grams, glucose 8.5 grams, nucleotide potassium and deoxynucleotide potassium are pulverized, mix the nutrient made from glucose; Perhaps refer to: get nucleotide potassium and deoxynucleotide potassium 2 grams, glucose 8 grams, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix the nutrient made from glucose; Perhaps refer to: get nucleotide potassium and deoxynucleotide potassium 2.5 grams, glucose 7.5 grams, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix the nutrient made from glucose; Perhaps refer to: get nucleotide potassium and deoxynucleotide potassium 3 grams, glucose 7 grams, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix the nutrient made from glucose; Perhaps refer to: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 1.5 grams, glucose 4.5 grams, amino acid 4 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix the nutrient made from glucose.
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| CN103960021A (en) * | 2014-05-15 | 2014-08-06 | 周萍萍 | Method for interplanting strawberries in vineyard |
| CN104798572A (en) * | 2015-04-08 | 2015-07-29 | 芜湖县能胜农业科技有限公司 | Strawberry planting method |
| CN105103881A (en) * | 2015-08-21 | 2015-12-02 | 安徽阳光雨露农业生态园有限公司 | Planting method for strawberries |
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