Summary of the invention
Object of the present invention overcomes above-mentioned deficiency exactly, a kind of activeconstituents in composition that reduced is provided, reasonable ratio, its effect still can meet or exceed the patent No. and be: the production method of the Rhizomatic traditional Chinese medicine of the patent of invention of a kind of nutrient substance that promotes growth of root stock traditional Chinese medicinal materials of " 201010514219.5 " and preparation method thereof.
The present invention is achieved in that
Promote the nutrient substance of growth of root stock traditional Chinese medicinal materials, it mainly by nucleosides hydrochlorate, in trace element form, in ideal nutrient composition, do not comprise glucose or/and amino acid, its weight percent is: nucleosides hydrochlorate 30-70%, middle micro-30-70%.
The nutrient substance that promotes growth of root stock traditional Chinese medicinal materials, screening formulation weight percent is: nucleosides hydrochlorate 40-60%, middle micro-30-70%.
Promote the nutrient substance of growth of root stock traditional Chinese medicinal materials, it is characterized in that nucleosides hydrochlorate refers to: after Yeast Nucleic Acid basic hydrolysis, obtain: 5 '-adenylic acid (AMP) dipotassium, 5 '-bird adenylic acid (AMP) dipotassium, 5 '-cytidylic acid dipotassium, the sylvite of four kind 5 '-mixed nucleotides of 5 '-uridylic acid dipotassium.
Promote the nutrient substance of growth of root stock traditional Chinese medicinal materials, nucleosides hydrochlorate refers to: nucleosides hydrochlorate refers to: after Yeast Nucleic Acid basic hydrolysis, obtain: 5 '-adenylic acid (AMP) disodium, 5 '-bird adenylic acid (AMP) disodium, 5 '-cytidylic acid disodium, the sodium salt of four kind 5 '-mixed nucleotides of 5 '-uridine monophosphate disodium.
The nutrient substance that promotes growth of root stock traditional Chinese medicinal materials, middle trace element refers to: Mn, Mo, Fe, Mg, Zn, specifically: manganous sulfate, ammonium molybdate, ferrous sulfate, Magnesium Stearate or sal epsom, zinc sulfate.
The nutrient substance that promotes growth of root stock traditional Chinese medicinal materials, middle micro-ratio is Mn: Mo: Fe: Mg: Zn=2: 1: 2: 1: 2.
Promote a nutrient substance for growth of root stock traditional Chinese medicinal materials, it also comprises weighting agent: one or more of starch, dextrin, Mierocrystalline cellulose, polyvidone, Magnesium Stearate, calcium sulfate or sal epsom.
The nutrient substance that promotes growth of root stock traditional Chinese medicinal materials, the weight percent of nucleosides hydrochlorate, middle trace element and weighting agent is: nucleosides hydrochlorate 10-30 %, middle micro-10-30%, weighting agent 40-80%.
Promote the nutrient substance of growth of root stock traditional Chinese medicinal materials, weighting agent is: one or more of W-Gum, dextrin, Mierocrystalline cellulose, Magnesium Stearate, sal epsom or zinc sulfate, the weight percent of nucleosides hydrochlorate, trace element and weighting agent is: nucleosides hydrochlorate 15-20%, middle micro-15-20%, weighting agent 40-70%.
Promote the preparation method of the nutrient substance of growth of root stock traditional Chinese medicinal materials, nutrient substance mainly by nucleosides hydrochlorate, in trace element form, in ideal nutrient composition, do not comprise glucose or/and amino acid, its weight percent is: nucleosides hydrochlorate 30-70%, middle micro-30-70%, by nucleosides hydrochlorate, middle trace element mixes, pulverizes, and crosses 50-200 mesh sieve.
The nutrient substance preparation method who promotes growth of root stock traditional Chinese medicinal materials, screening formulation weight percent is: nucleosides hydrochlorate 40-60%, middle micro-40-60%.
Promote the preparation method of the nutrient substance of growth of root stock traditional Chinese medicinal materials, nucleosides hydrochlorate refers to: after Yeast Nucleic Acid basic hydrolysis, obtain: 5 '-adenylic acid (AMP) dipotassium, 5 '-bird adenylic acid (AMP) dipotassium, 5 '-cytidylic acid dipotassium, the sylvite of four kind 5 '-mixed nucleotides of 5 '-uridylic acid dipotassium.
Promote the preparation method of the nutrient substance of growth of root stock traditional Chinese medicinal materials, nucleosides hydrochlorate refers to: nucleosides hydrochlorate refers to: after Yeast Nucleic Acid basic hydrolysis, obtain: 5 '-adenylic acid (AMP) disodium, 5 '-bird adenylic acid (AMP) disodium, 5 '-cytidylic acid disodium, the sodium salt of four kind 5 '-mixed nucleotides of 5 '-uridine monophosphate disodium.
The preparation method who promotes the nutrient substance of growth of root stock traditional Chinese medicinal materials, middle trace element refers to: Mn, Mo, Fe, Mg, Zn, specifically: manganous sulfate, ammonium molybdate, ferrous sulfate, Magnesium Stearate, sal epsom or zinc sulfate.
The preparation method who promotes the nutrient substance of growth of root stock traditional Chinese medicinal materials, middle micro-ratio is Mn: Mo: Fe: Mg: Zn=2: 1: 2: 1: 2.
Promote the preparation method of the nutrient substance of growth of root stock traditional Chinese medicinal materials, in nutrient substance, also comprise weighting agent: one or more of starch, dextrin, Mierocrystalline cellulose, polyvidone, Magnesium Stearate, calcium sulfate or sal epsom, add the weighting agent of pulverizing to mix with trace element in nucleosides hydrochlorate, the weight percent of nucleosides hydrochlorate and middle trace element and weighting agent is: nucleosides hydrochlorate 10-30 %, middle micro-10-30%, weighting agent 20-80%, Magnesium Stearate, sal epsom in the Magnesium Stearate in middle trace element, sal epsom and weighting agent are equal to.
The preparation method who promotes the nutrient substance of growth of root stock traditional Chinese medicinal materials, the weight percent of nucleosides hydrochlorate, middle trace element and weighting agent is: nucleosides hydrochlorate 10-30 %, middle micro-10-30%, weighting agent 40-80%.
Promote the preparation method of the nutrient substance of growth of root stock traditional Chinese medicinal materials, weighting agent is: one or more of W-Gum, dextrin, Mierocrystalline cellulose, Magnesium Stearate, sal epsom or zinc sulfate, the weight percent of nucleosides hydrochlorate, trace element and weighting agent is: nucleosides hydrochlorate 15-20%, middle micro-15-20%, weighting agent 40-70%.
The production method of Rhizomatic traditional Chinese medicine, in its process of growth, use nutrient substance: described nutrient substance mainly by nucleosides hydrochlorate, in trace element form, in ideal nutrient composition, do not comprise glucose or/and amino acid, its weight percent is: nucleosides hydrochlorate 30-70%, middle micro-30-70%; The application process of described nutrient substance is: with spraying after water dissolution.
The production method of Rhizomatic traditional Chinese medicine, its feature is being: by the concentration spraying after water dissolution, be 100-400ppm.
The production method of Rhizomatic traditional Chinese medicine, middle trace element refers to: Mn, Mo, Fe, Mg, Zn, specifically: manganous sulfate, ammonium molybdate, ferrous sulfate, Magnesium Stearate, sal epsom or zinc sulfate.
The production method of Rhizomatic traditional Chinese medicine, middle micro-ratio is Mn: Mo: Fe: Mg: Zn=2: 1: 2: 1: 2.
The production method of Rhizomatic traditional Chinese medicine, it also comprises weighting agent: one or more of starch, dextrin, Mierocrystalline cellulose, polyvidone, Magnesium Stearate, calcium sulfate or sal epsom, the weight percent of nutrient substance composition is: nucleosides hydrochlorate 10-30 %, middle micro-10-30%, weighting agent 40-80%, Magnesium Stearate, sal epsom in the Magnesium Stearate in middle trace element, sal epsom and weighting agent are equal to.
The production method of Rhizomatic traditional Chinese medicine, be the opportunity of using of described nutrient substance: at the bud stage of rhizome traditional Chinese medicinal materials or/and initial bloom stage and full-bloom stage each once.
The production method of Rhizomatic traditional Chinese medicine, be the opportunity of using of described nutrient substance: at the germination period of rhizome traditional Chinese medicinal materials or/and bud stage or/and initial bloom stage and full-bloom stage or/and first 2 months of harvesting time each once.
The production method of Rhizomatic traditional Chinese medicine, the consumption of described nutrient substance is every mu of 2-8 gram, by water-soluble water consumption, is every mu of 20-40 liter.
The production method of Rhizomatic traditional Chinese medicine, the consumption of described nutrient substance is each every mu of 15-30 gram, by water-soluble water consumption, is each every mu of 20-40 liter.
The production method of described Rhizomatic traditional Chinese medicine, the Chinese medicinal materials size of results evenly, its output is than the high 20-80% of produced in conventional processes, its Contents of Main Components is than the high 10-50% of produced in conventional processes, and the Chinese medicinal materials of results is made to high-quality medicine materical crude slice or extracting effective components yield than the high 15-49% of the Chinese medicinal materials of produced in conventional processes.
Weighting agent of the present invention: starch refers to: green starch, yam starch, wheat starch, sweet potato starch, W-Gum, water caltrop starch, Lotus Root Starch, Chinese Water Chestnut Starch etc., preferably W-Gum.Dextrin refers to: white dextrin, yellow starch gum and Britain glue, cyclodextrin.Mierocrystalline cellulose refers to: methylcellulose gum, Vltra tears, Natvosol, carboxymethyl cellulose etc.
In the preparation of nutrient substance provided by the present invention, take nucleic acid as raw material: the one, take commercially available nucleic acid as raw material, 1., the hydrolysis of the nucleic acid ratio mixed nucleus bronsted lowry acids and bases bronsted lowry liquid that is 1:20-30 according to nucleic acid, buck weight ratio the 2nd, it is raw material that the mycelium from fermentation plant fermentation adopts the nucleic acid of dry method or wet production:, alkali lye is 5.5-7N, under 30-45 ℃ of condition, stirs 10-14 hours, obtain nucleosides hydrochlorate and deoxynucleoside acid salt solution, filter, filtrate is adjusted pH8-8.5, concentrating under reduced pressure;
2., decolouring, dry: concentrated solution is added to activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 10-25%;
3., crystallization: dissolve nucleosides hydrochlorate and the deoxynucleoside hydrochlorate after doing with clear water, its solution adds alcohol and separates out, crystallization suction filtration is dry, obtain white nucleosides hydrochlorate and deoxynucleoside hydrochlorate (it is impurity that the mycelium from fermentation plant fermentation adopts the water soluble mixt that also contains that the nucleic acid of dry method or wet production is raw material);
4., get middle trace element;
, trace element in nucleosides hydrochlorate is mixed, is pulverized, sieve.
In nucleic acid hydrolysis process described in this preparation method, alkali lye used is sodium hydroxide or potassium hydroxide solution.
Take commercially available nucleic acid as raw material, impure few after hydrolysis.
The 3rd, commercially available: nucleosides hydrochlorate mainly refers to: 5 '-adenylic acid (AMP) disodium or potassium, 5 '-bird adenylic acid (AMP) disodium or potassium, 5 '-cytidylic acid disodium or potassium, sodium salt or the sylvite of four kind 5 '-mixed nucleotides of 5 '-uridine monophosphate disodium or potassium; Deoxynucleoside hydrochlorate refers to: 5 '-Desoxyadenosine acid disodium or potassium, 5 '-deoxidation bird adenylic acid (AMP) disodium or potassium, 5 '-Deoxyribose cytidine acid disodium or potassium, 5 '-deoxyuridine acid disodium,, sodium salt or the sylvite of five kind 5 '-deoxidation mixed nucleotides of 5 '-deoxythymidine acid disodium or potassium.
The source of nucleosides hydrochlorate and deoxynucleoside hydrochlorate is for take nucleic acid as raw material, that to adopt the nucleic acid of dry method or wet production be raw material for mycelium from fermentation plant fermentation, this kind of nucleic acid be take Yeast Nucleic Acid (RNA) as a small amount of thymus nucleic acid of main mixing (DNA), with alkali lye hydrolysis, makes.
Beneficial effect of the present invention:
Because the present invention is not containing glucose, reduced composition activeconstituents, so more economize in raw materials and cost.
Because the present invention contains middle trace element, coordinate nucleosides hydrochlorate, reasonable ratio, promotes the growth of Rhizomatic traditional Chinese medicine more targeted specifically.
Although the present invention has reduced activeconstituents, its effect does not still subtract and even produces better effect.
The present invention is not particularly containing glucose, glucose easily absorbs moisture deliquescence, the nutrient substance that contains glucose is placed the deliquescence that absorbs water more than a year under general condition of storage, there is product caking, make nucleosides hydrochlorate, cause quality of products even to lose efficacy, so, the invention solves the problem that is difficult for standing storage of nutrient substance.
Because the present invention also comprises weighting agent: one or more of starch, dextrin, Mierocrystalline cellulose, polyvidone, Magnesium Stearate, calcium sulfate or sal epsom etc., the adding to have adapted to of weighting agent are generally not less than the relevant regulations of 20 grams as the foliage fertilizer minimum package of merchandise sales; Filler starch, dextrin, Mierocrystalline cellulose etc. also possess certain viscosity, after spraying, make nutrient substance be attached to blade face and the plant surface time longer, make its action effect better; Weighting agent polyvidone, Magnesium Stearate, calcium sulfate or sal epsom etc. have dispersiveness and oilness, can extend the quality guaranteed period of nutrient substance.
Purposes of the present invention is: as fertilizer sources is particularly as foliage fertilizer or nutrient substance or the application of plant-growth regulator in the growth that promotes Rhizomatic traditional Chinese medicine; Or the production that is applied to Rhizomatic traditional Chinese medicine can improve its Yield and quality, quality, enhancing resistance, uniformity coefficient, reduce nuisance content, cost-saving, increase income, synergy.
The present invention is applied to the production of Rhizomatic traditional Chinese medicine, with strong points, can improve its Yield and quality, quality, enhancing resistance, uniformity coefficient, reduce nuisance content, cost-saving, increase income, synergy.
So product of the present invention: save, environmental protection (nontoxic, harmless, noresidue, without any adverse side effect), comprehensive to the purposes of Rhizomatic traditional Chinese medicine, increase production, increase income, synergy, enhancing resistance, improves quality effective.
The present invention and No. 201010514219.5 patents, traditional product and method contrast tables 1
The present invention and No. 201010514219.5 patents, traditional product and method contrast tables 2
The present invention implements to spray agency part effect in Table 3 to concrete crop
Note: content's index detection method is carried out with reference to national standard or industry standard, content is pressed dry product and is calculated
Embodiment:
Embodiment 1: take 3 grams of nucleosides hydrochlorates, and middle micro-2 grams, nucleosides hydrochlorate, middle trace element are pulverized, cross 60 mesh sieves, mix, obtain nutrient substance.
Embodiment 2: take 5 grams of nucleosides hydrochlorates, and middle micro-5 grams, nucleosides hydrochlorate, middle trace element are pulverized, cross 100 mesh sieves, obtain nutrient substance.
Embodiment 3: take 9 grams of nucleosides hydrochlorates, and 15 grams of W-Gums, middle micro-6 grams, by nucleosides hydrochlorate, middle trace
Element is pulverized, and crosses 80 mesh sieves, adds W-Gum to mix, and obtains nutrient substance.
Embodiment 4: the root of herbaceous peony: spray twice, for embodiment 1 nutrient substance for initial bloom stage, be watered 30 kilograms for the first time, evenly spray on one mu of root of herbaceous peony.Be full-bloom stage for the second time, after the~inferior spraying 7 days with embodiment 2 nutrient substances, be watered 20 kilograms, evenly spraying on one mu of root of herbaceous peony can (spray every year 2 times, mu volume increase surpasses 200 kilograms of volume increase, 23 % left and right.
Embodiment 5: the root of Dahurain angelica: spray twice, for the first time also can initial bloom stage in the flower bud phase, and with embodiment 3 nutrient substances, be watered 30 kilograms, evenly spray on one mu of root of Dahurain angelica.Full-bloom stage, with embodiment 2 nutrient substances, is watered 20 kilograms for the second time, evenly sprays on one mu of root of Dahurain angelica mu volume increase 16 % left and right.
Embodiment 6: the red sage root: spray twice, use 6 grams at every turn, every 3 grams are watered 18 kilograms, spray on one mu of red sage root, and the time that sprays sprays for the first time between June, spray for the second time 120 kilograms of mu volume increase between July.
Embodiment 7: Root of Manyflower Glorybower: within first 2 months of germination period, initial bloom stage and full-bloom stage and harvesting time, respectively spray once.Volume increase 30%~40%.Leaf of Indigowoad volume increase is more than 30%.
Embodiment 8: the Chinese medicinal materials of results can be made the evenly Chinese medicinal materials high-quality medicine materical crude slice of (evening ratio 60-80%) of size; Extracting effective components yield is than the Chinese medicinal materials of produced in conventional processes high (in Table 3).