CN101786914A - Microorganism fertilizer for promoting rice production, preparation method and application thereof - Google Patents
Microorganism fertilizer for promoting rice production, preparation method and application thereof Download PDFInfo
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Abstract
The invention also relates to a microorganism fertilizer. The composite microorganism preparation comprises the components based on the volume ratio: 5-15% of photosynthetic bacteria, 10-20% of bacillus subtilis, 15-35% of saccharomycete, 25-45% of thermophilic bacillus and 3-15% of actinomycete. The invention also relates to the application of the microorganism fertilizer in the aspects of improving the resistance of seeds, preventing plant diseases and insect pests, purifying the water quality of rice field and increasing the rice production, and a using method thereof. The invention also relates to the application of the microorganism fertilizer in the aspect of accelerating rice stubble rotten degree, and a using method thereof.
Description
Technical field
The invention belongs to fertilizer field, be specifically related to a kind of microbial fertilizer application aspect the paddy rice plantation and preparation method thereof.
Background technology
Bread is the staff of life, and food is earlier with the rice.About 4.2 hundred million mu at the total sown area of China in paddy rice account for 30% of the total sown area of food crop, and output has accounted for 40% of total output of grain, is the first food crop of China.Though successively by the improvement of seed variety, realized the secondary leap of output, the quality of depending merely on seed improves quality and raising output is not enough, how to improve depend on for existence the quality of soil of seed and is only more The key factor.Rice terrace excessively uses chemical fertilizer at present, and the use of ignoring fertilizer, microbial-bacterial fertilizer causes soil compaction, and air permeability is poor, and it is bad to cause rice root to be grown; Paddy rice first crop process of growth produces from poisonous substance matter influences the rice root growth; The paddy rice rice stubble rots process because of lacking corresponding microorganism, and the speed of rotting is slow, and it is many to produce objectionable impurities, influences the rice root growth; Use that weedicide is improper, herbicide damage, fertilizer damage be serious.These phenomenons only show improves the soil, and elimination is hardened, and eliminates the toxic substance in the soil, and the beneficial microorganism and the organic matter that increase in the soil just can impel paddy rice significantly to increase production.Realize the dual leap of rice quality and output.
Summary of the invention
The purpose of this invention is to provide a kind of microbial fertilizer that is applied to the paddy rice aspect.
The present invention also aims to provide the preparation method of this microbial fertilizer.
The present invention also aims to provide the application of this microbial fertilizer preparation aspect pre-preventing disease and pest, purification water quality of paddy field, raising rice yield.
The present invention also aims to provide this microbial fertilizer preparation accelerating the application of rice stubble aspect becoming thoroughly decomposed.
The present invention also aims to provide the using method of this microbial fertilizer.
The invention provides a kind of microbial fertilizer, is to be mixed by photosynthetic bacterium, subtilis, yeast, bacillus acidocldarius, actinomycetic zymocyte liquid.Wherein, the volume of described each zymocyte liquid by volume part meter be preferably: photosynthetic bacterium 5～15%, subtilis 10～20%, yeast 15～35%, bacillus acidocldarius 25～45%, actinomycetes 3～15%.
The unit living bacteria count is not less than 500,000,000/milliliter in the various zymocyte liquids.
Wherein said photosynthetic bacterium is preferably swamp Rhodopseudomonas (Rhodopseudomonaspalustris); Described subtilis (Bacillus subtilis); Described yeast is preferably cereuisiae fermentum (Saccharomyces cerevisiae); Described bacillus acidocldarius is bacstearothermophilus (Bacillus thermophilus); Described actinomycetes are preferably Jingyang streptomycete (Streptomyces jingyangesis).
The present invention also provides the preparation method of described microbial fertilizer.This preparation method comprises the steps:
(1) respectively photosynthetic bacterium, subtilis, yeast, bacillus acidocldarius, actinomycetic female kind are inoculated in the one-level substratum and carry out the one-level cultivation, obtain first class inoculum;
(2) respectively photosynthetic bacterium, subtilis, yeast, bacillus acidocldarius, actinomycetic first class inoculum are inoculated in secondary medium and carry out the secondary cultivation, obtain zymocyte liquid;
(3) with each zymocyte liquid in proportion mixing obtain composite bacteria.
Wherein, inoculum size was 0.5%～1% when the photosynthetic bacterium one-level was cultivated in the step (1), and is preferred 0.5%, and cultivating pH is 6～10, and culture temperature is 28～36 ℃, and intensity of illumination is 3000～4000 luxs, and incubation time is 7～10 days; Inoculum size was 0.5%～1% when the subtilis one-level was cultivated, and is preferred 0.5%, and cultivating pH is 6～8, and culture temperature is 28～34 ℃, and incubation time is 2～3 days; Inoculum size was 0.5%～1% when the yeast one-level was cultivated, and is preferred 0.5%, and cultivating pH is 4～7, and culture temperature is 25～30 ℃, and incubation time is 1～2 day; Inoculum size was 0.5%～1% when the bacillus acidocldarius one-level was cultivated, and is preferred 0.5%, and cultivating pH is 6～8, and culture temperature is 38～45 ℃, and incubation time is 2～5 days; Inoculum size was 0.5%～1% when the actinomycetes one-level was cultivated, and is preferred 0.5%, and cultivating pH is 6～8, and culture temperature is 27～32 ℃, and incubation time is 5～7 days;
The photosynthetic bacterium secondary is cultivated and is preferably the intermittent type aerated culture in the step (2), and inoculum size is 5%～15%, and is preferred 10%, and cultivating pH is 6～10, and culture temperature is 28～36 ℃, and intensity of illumination is 3000～4000 luxs, and incubation time is 7～10 days; The subtilis secondary is cultivated and to be preferably aerated culture, and inoculum size is 5%～15%, and is preferred 10%, and cultivating pH is 6～8, and culture temperature is 28～32 ℃, and incubation time is 2～5 days; The yeast secondary is cultivated and to be preferably aerated culture, and inoculum size is 5%～15%, and is preferred 10%, and cultivating pH is 4～7, and culture temperature is 25～30 ℃, and incubation time is 1～2 day; The bacillus acidocldarius secondary is cultivated and to be preferably aerated culture, and inoculum size is 5%～15%, and is preferred 10%, and cultivating pH is 6～8, and culture temperature is 38～45 ℃, and incubation time is 2～5 days; The actinomycetes secondary is cultivated and to be preferably aerated culture, and inoculum size is 5%～15%, and is preferred 10%, and cultivating pH is 6～8, and culture temperature is 27～32 ℃, and incubation time is 5～7 days.
The present invention also provides this microbial fertilizer in preparation prevention rice pest, the application that purify water quality of paddy field simultaneously, improves the rice yield aspect.
The present invention also aims to provide the application of described microbial fertilizer in preparation quickening rice stubble digest process.
The present invention also provides the using method of described microbial fertilizer, it is characterized in that adding in proportion when rice seed soaking, nursery stage and vegetative period, harvesting aftertreatment rice stubble described microbial fertilizer, the use when soaking seed, spraying and irritating root.
Microbial fertilizer of the present invention provide a kind of can be after rice paddy seed carries out presoaking and germinating, can break seed dormancy fast, promote seed germination, strengthen seed resistance, for guaranteeing that full stand, strong sprout lay a solid foundation, simultaneously for prevent and treat disease, suppress insect pest generation, the resistance etc. that improves crop has played good effect, is the microbial fertilizer of an economical and effective well stimulation.After the rice field applies the present invention, can P and K decomposing fixed nitrogen, and change trace element in the soil, can produce the various plants mediator agent, greatly change the crop microcirculating state, promote absorption and conversion nutrient, strengthen photosynthesis, quicken cell fission speed, accelerate cell and expand, promote growth, improve output, improve quality, have sterilization simultaneously and press down the worm effect, all produce effect for common Achlya spp disease, bakanae disease, banded sclerotial blight, rice blast.Microbial fertilizer of the present invention, state of the art is reached advanced world standards, is by multiple beneficial living microorganism process scientific matching, is composited jointly, has really reached pleiotropy, and the unit living bacteria count is not less than 2,000,000,000/milliliter, considerably beyond national standard.
Useful technique effect and using method are embodied in following several aspect:
After the present invention's seed soaking, can break seed dormancy fast, promote seed germination, strengthen seed resistance, guarantee that the neat seedling of seedling is strong, the seedling rate height; Effectively improve soil physical and chemical property, prevent the soil belt poison, prevent and treat the generation of soil-borne disease, Achlya spp disease, bakanae disease, banded sclerotial blight, rice blast; Impel root growth, main root is sturdy, and fibrous root is many; Cold-resistant drought-resistance ability strengthens; Purify water, blue bath obviously tails off; Short control combination is steadily and surely grown paddy rice, strengthens photosynthesis, and short root increases tiller, makes the early growth and quick tiller of tillering, and reaches that seedling is strong, fringe is many, fringe is big, prevent remaining green when it is due to become yellow and ripe late-maturing with take off fertile early ageing; Improve output about 17%, the improvement quality.
After handling rice stubble and become thoroughly decomposed with microbial fertilizer of the present invention, can accelerate the rice stubble decomposition rate, reduce obnoxious flavour and discharge, decompose paddy rice, solve the paddy rice continuous cropping from poisonous substance matter; Increase the soil beneficial microbe colony, suppress soil disease and take place, improve the rice shoot survival rate, strong rice root; Purifying water body reduces harmful organism growths such as blue-green algae, builds ecological water body, creates paddy rice healthy growth environment; Improve utilization rate of fertilizer, promote paddy growth, increase rice yield, improve rice quality.
Description of drawings (among the figure " demonstration group " for having used design sketch of the present invention, " control group " be not for using design sketch of the present invention)
Fig. 1 microbial inoculum technological process of production figure.
Fig. 2 has used the present invention and has not used the comparison diagram in seedling stage of the present invention, and rice seedling rate height of the present invention is used in the left side, seedling is neat, seedling is strong, leaf color jade green, well developed root system, strong resistance; The right side does not use that seedling rate of the present invention is low, uneven, the leaf look yellowish green.
Fig. 3-1 has used rice root fibrous root of the present invention white, strong, long, and vitality is strong, can remain vigorous dietetic alimentation ability, and seedling-slowing stage is short behind the root system field-transplanting like this according to experts' analysis;
Fig. 3-2 does not use paddy rice fibrous root of the present invention short, thin, in vain must be few, illustrate most of root system or gradually forfeiture absorb nutrient ability and withered, transplanting seedling time is longer behind this type of root system field-transplanting of analysis expert.
Fig. 4-1 used rice field of the present invention, and field blue-green algae material is few, and dry weight on average is 0.305kg/m
2, and all atrophys.
Fig. 4-2 does not use rice field of the present invention, and field blue-green algae material is many, and average dry weight is 3.02kg/m
2
It is high that Fig. 5-1 uses paddy rice The mean tillering rate of the present invention more not use, and cane is sturdy, healthy.
Fig. 5-2 does not use rice tillering of the present invention less, and cane has the canescence mould.
Fig. 6-1 use paddy rice arrow leaf of the present invention is big, photosynthetic by force, heading is neat, growing way is good.
Fig. 6-2 does not use that paddy rice early ageing of the present invention is serious, heading is irregular, the rice blast big area takes place, a little less than the growing way.
Embodiment
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.
Each culture of strains base
The one-level substratum of swamp Rhodopseudomonas is: MgSO
47H
2O 0.2g, (NH
4)
2SO
41.0g, NaHCO
35.0g, K
2HPO
40.5g, NaCl 0.2g, peptone 1.5g; Each composition is dissolved in the 400ml distilled water, uses H
3PO
4And Na
2CO
3Transfer pH 7.1, add distilled water again, sterilized 10 minutes for 115 ℃ to 1000ml; Secondary medium is: Na
2CO
33g, NaCl 0.1g, (NH
4)
2SO
40.3g, MgSO
40.2g, KH
2PO
30.5g, K
2HPO
30.3g, ferric ammonium citrate 7ml, peptone 1.2g, be settled to 1000ml with distilled water, use H
3PO
4And Na
2CO
3Transferring pH is 7.2.
The one-level substratum of subtilis is: extractum carnis 0.3%, peptone 1.0%, NaCl 0.5%, be settled to 1000ml with distilled water, and pH 7.0; Secondary medium is: extractum carnis 1.0%, peptone 1.0%, NaCl 0.5%, agar 2.0% are settled to 1000ml with distilled water, pH7.0.
The one-level substratum of cereuisiae fermentum is: potato juice 800ml, glucose 20g, agar 20g, be settled to 1000ml with distilled water, and pH 6.0; Secondary medium: yeast extract paste 1%, peptone 2%, glucose 2%, MgSO
40.06%, K
2HPO
40.25%, water supplies 100%, pH6.0.
The one-level substratum of bacstearothermophilus is: extractum carnis 0.3%, peptone 1.0%, NaCl 0.5%, be settled to 1000ml with distilled water, and pH 7.0; Secondary medium is: extractum carnis 1.0%, peptone 1.0%, NaCl 0.5%, agar 2.0% are settled to 1000ml with distilled water, pH7.0.
The one-level substratum of Jingyang streptomycete is: KNO
31g, Zulkovsky starch 20g, K
2HPO
40.5g, MgSO
40.5g, NaCl 0.5g, FeSO
40.01g agar 20g is settled to 1000ml with distilled water, pH7.2; Secondary medium is KNO
30.8g, Zulkovsky starch 8g, glucose 5g, yeast water 5g, K
2HPO
40.3g, MgSO
40.2g, NaCl 0.4g, FeSO
40.05g, be settled to 1000ml with distilled water, pH7.2.
The preparation method of embodiment 1 complex microorganism preparations
(1) gets each 3L of eggplant bottle bacterium of swamp Rhodopseudomonas, subtilis, cereuisiae fermentum, bacstearothermophilus and Jingyang streptomycete, be inoculated in respectively and carry out one-level in the one-level substratum of each bacterial classification of 300L and cultivate, obtain the first class inoculum of each component;
(2) get each 300L of first class inoculum of swamp Rhodopseudomonas, subtilis, cereuisiae fermentum, bacstearothermophilus and Jingyang streptomycete, be inoculated in respectively and carry out secondary in the secondary medium of each bacterial classification of 3000L and cultivate, obtain the zymocyte liquid of each component;
(3) zymocyte liquid of each component is carried out compound in ratio mixing in the 10000L container for storing liquid of swamp Rhodopseudomonas 12%, subtilis 10%, cereuisiae fermentum 30%, bacstearothermophilus 43%, Jingyang streptomycete 5%, obtain the 10000L composite bacteria.
In step (2), swamp Rhodopseudomonas is the intermittent type aerated culture; Subtilis is an aerated culture; Cereuisiae fermentum is an aerated culture; Bacstearothermophilus is an aerated culture; The Jingyang streptomycete is an aerated culture.
The preparation method of embodiment 2 complex microorganism preparations
(1) gets each 5L of eggplant bottle bacterium of swamp Rhodopseudomonas, subtilis, cereuisiae fermentum, bacstearothermophilus and Jingyang streptomycete, be inoculated in respectively and carry out one-level in the one-level substratum of each bacterial classification of 500L and cultivate, obtain the first class inoculum of each component;
(2) get each 500L of first class inoculum of swamp Rhodopseudomonas, subtilis, cereuisiae fermentum, bacstearothermophilus and Jingyang streptomycete, be inoculated in respectively and carry out secondary in the secondary medium of each bacterial classification of 5000L and cultivate, obtain the zymocyte liquid of each component;
(3) zymocyte liquid of each component is carried out compound in ratio mixing in the 10000L container for storing liquid of swamp Rhodopseudomonas 5%, subtilis 15%, cereuisiae fermentum 30%, bacstearothermophilus 40%, Jingyang streptomycete 10%, obtain the 10000L composite bacteria.
In step (2), swamp Rhodopseudomonas is the intermittent type aerated culture; Subtilis is an aerated culture; Cereuisiae fermentum is an aerated culture; Bacstearothermophilus is an aerated culture; The Jingyang streptomycete is an aerated culture.
The preparation method of embodiment 3 complex microorganism preparations
(1) gets each 6L of eggplant bottle bacterium of swamp Rhodopseudomonas, subtilis, cereuisiae fermentum, bacstearothermophilus and Jingyang streptomycete, be inoculated in respectively and carry out one-level in the one-level substratum of each bacterial classification of 600L and cultivate, obtain the first class inoculum of each component;
(2) get each 600L of first class inoculum of swamp Rhodopseudomonas, subtilis, cereuisiae fermentum, bacstearothermophilus and Jingyang streptomycete, be inoculated in respectively and carry out secondary in the secondary medium of each bacterial classification of 6000L and cultivate, obtain the zymocyte liquid of each component;
(3) zymocyte liquid of each component is carried out compound in ratio mixing in the 10000L container for storing liquid of swamp Rhodopseudomonas 8%, subtilis 12%, cereuisiae fermentum 35%, bacstearothermophilus 42%, Jingyang streptomycete 3%, obtain the 10000L composite bacteria.
In step (2), swamp Rhodopseudomonas is the intermittent type aerated culture; Subtilis is an aerated culture; Cereuisiae fermentum is an aerated culture; Bacstearothermophilus is an aerated culture; The Jingyang streptomycete is an aerated culture.
Omnidistance using method of embodiment 4 paddy growths and effect
1. presoaking and germinating:, can break seed dormancy fast, promote seed germination, strengthen seed resistance with 50-100 times of diluent seed soaking.
2. culturing and transplanting seedlings: before transplanting 7-8 days, 300 times of diluents sprayed, and guarantee that the neat seedling of seedling is strong, the seedling rate height.
3. irritate with water before the rice transplanting: evenly be sprayed on diluent of the present invention in the rice field, mu consumption 1000-2000ml, can effectively improve soil physical and chemical property, prevent the soil belt poison, prevent and treat the generation of soil-borne disease, prevention of water seedling blight of rice disease, bakanae disease, banded sclerotial blight, rice blast; Purify water, blue bath obviously tails off.
4. jointing stage, tillering phase, booting filling stage are impelled root growth respectively with 600 times of diluent foliage spray, and main root is sturdy, and fibrous root is many; Cold-resistant drought-resistance ability strengthens.
Omnidistance with behind the present invention, short control combination is steadily and surely grown paddy rice, strengthens photosynthesis, and short root increases tiller, makes the early growth and quick tiller of tillering, and reaches that seedling is strong, fringe is many, fringe is big, prevent remaining green when it is due to become yellow and ripe late-maturing with take off fertile early ageing; Improve output about 17%, the improvement quality.
Embodiment 5 accelerates using method and the effect that rice stubble becomes thoroughly decomposed
Before the soil is about to turn over, microbial fertilizer of the present invention evenly is sprayed onto in the rice terrace 2000 milliliters/mu; Perhaps before turning over, execute in the rice terrace 2000 milliliters/mu along with irrigating microbial inoculum dashed.Can accelerate the rice stubble decomposition rate, reduce obnoxious flavour and discharge, decompose paddy rice, solve the paddy rice continuous cropping from poisonous substance matter; Increase the soil beneficial microbe colony, suppress soil disease and take place, improve the rice shoot survival rate, strong rice root; Purifying water body reduces harmful organism growths such as blue-green algae, builds ecological water body, creates paddy rice healthy growth environment; Improve utilization rate of fertilizer, promote paddy growth, increase rice yield, improve rice quality.
Precaution during embodiment 6 uses
1, forbids to use with any medicine.
2, slowly unscrew earlier bottle cap during uncork and discharge shielding gas, prevent that the bacterium hydrorrhea from going out.
3, the sealing of normal temperature lucifuge is stored, with before shaking up.
4, diluting water wants clean, and water temperature is not higher than 40 ℃.
5, used container, equipment must clean up.
6, this product safety, nontoxic, have no side effect.
Experimental example 1: the application of microbial preparation of the present invention aspect paddy rice
Nan Mazhuan village, Lankao, Henan is the best place of rice cultivation always.2006, the present invention has obtained using widely on the paddy rice of the Nan Ma village, and obtained unusual effect, be embodied in: soil has obtained improvement, the seedling rate height, Miao Qi, Miao Zhuan (as Fig. 2), well developed root system is (as Fig. 3-1,3-2), effectively controlled the harm of striped blight and planthopper, made thousand seed weight increase 0.7g, the average yield per mu of control group is 396.65kg, and the average yield per mu of demonstration group is 466.55kg, and than control group mu volume increase 69.9kg, output improves 17%, the omnidistance usage quantity that reduces by 30% fertilizer and pesticide, rice milling yield is high and rice matter is good, and wraps in the ground price hearing at the rice of purchasing of Held in Beijing, has won the expert, professor's a consistent favorable comment!
Experimental example 2: the application of microbial preparation of the present invention aspect paddy rice
Lankao, Henan in June, 2007 Nan Mazhuan paddy fields has broken out the big area blue-green alga bloom, company to part peasant household paddy field application by throwing them behind the present invention, make the blue-green algae in the rice terrace controlled.The outburst blue-green algae rice terrace and used in the rice terrace of the present invention, the plot that picked at random is one square metre, fish for suspended biological (comprising blue-green algae) in the water with grate, be placed on after the taking-up in the string bag and weigh behind the suspension drip-dry moisture: using field of the present invention blue-green algae material is 0.305kg (mean value), do not use rice field of the present invention, field blue-green algae material 3.02kg, and used the whole atrophys of blue-green algae of the present invention, water quality limpid (as Fig. 4-1,4-2).
Experimental example 3: the application of microbial preparation of the present invention aspect paddy rice
2008, Jiangsu Province's Yancheng City, scientific research, popularization, master of instruction carry out the on-the-spot product of surveying to the Xin Yang farm to using paddy rice of the present invention " force is educated round-grained rice No. 3 ": under same medium soil fertility condition on the lower side, under the situation of 5 kilograms of tillering fertilizer and every mu of average Shaoshis of ear manuer, after using comprehensive treating processs such as microbial inoculum is soaked seed, repair in the seedbed, filling root, foliage-spray, do not use rice tillering of the present invention less, and cane have the canescence mould; Use paddy rice figure The mean tillering rate of the present invention many, and cane is sturdy, health (as Fig. 5-1,5-2) than control group.Use every mu of number of productive ear of the present invention to reach 23.09 ten thousand, increased by 18.28% than untapped, every mu of per unit area yield is up to 678.54 kilograms, than untapped volume increase 15.47%.Expert conclusion: Rice Production is used this novel " microbial inoculum ", does not only increase user's cost, and drops into and reduce, and output increases, and abridged edition increases income significantly.
Experimental example 4: the application of microbial preparation of the present invention aspect paddy rice
Qiyang,hunan red soil experiment centre: sum up use paddy rice arrow leaf of the present invention big, photosynthetic strong, heading is neat, growing way is good, does not use that paddy rice early ageing of the present invention is serious, heading is irregular, the rice blast big area takes place, growing way weak (as Fig. 6-1,6-2).Use rice field of the present invention to promote the utilization ratio of soil phosphorus, potassium, significantly increased paddy rice effective fringe, grain number per spike, heavy, the thousand seed weight of the real grain of fringe, reduce paddy rice ghost rate, volume increase 10%-15%.
Claims (9)
1. a microbial fertilizer is characterized in that, is to be mixed by photosynthetic bacterium, subtilis, yeast, bacillus acidocldarius and actinomycetic zymocyte liquid.
2. microbial fertilizer according to claim 1, it is characterized in that the volume of described each zymocyte liquid by volume part is counted: photosynthetic bacterium 5～15%, subtilis 10～20%, yeast 15～35%, bacillus acidocldarius 25～45%, actinomycetes 3～15%.
3. microbial fertilizer according to claim 1 and 2 is characterized in that described photosynthetic bacterium is preferably swamp Rhodopseudomonas; Described yeast is preferably cereuisiae fermentum; Described bacillus acidocldarius is a bacstearothermophilus; Described actinomycetes are preferably the Jingyang streptomycete.
4. the preparation method of the arbitrary described microbial fertilizer of claim 1～3, this method comprises the steps:
(1) respectively photosynthetic bacterium, subtilis, yeast, bacillus acidocldarius, actinomycetic female kind are inoculated in the one-level substratum and carry out the one-level cultivation, obtain first class inoculum;
(2) respectively photosynthetic bacterium, subtilis, yeast, bacillus acidocldarius, actinomycetic first class inoculum are inoculated in secondary medium and carry out the secondary cultivation, obtain zymocyte liquid;
(3) with each zymocyte liquid mixing in proportion.
5. the preparation method of microbial fertilizer according to claim 4, it is characterized in that, inoculum size was 0.5%～1% when the photosynthetic bacterium one-level was cultivated in the described step step (1), preferred 0.5%, cultivating pH is 6～10, culture temperature is 28～36 ℃, and intensity of illumination is 3000～4000 luxs, and incubation time is 7～10 days; Inoculum size was 0.5%～1% when the subtilis one-level was cultivated, and is preferred 0.5%, and cultivating pH is 6～8, and culture temperature is 28～34 ℃, and incubation time is 2～3 days; Inoculum size was 0.5%～1% when the yeast one-level was cultivated, and is preferred 0.5%, and cultivating pH is 4～7, and culture temperature is 25～30 ℃, and incubation time is 1～2 husband; Inoculum size was 0.5%～1% when the bacillus acidocldarius one-level was cultivated, and is preferred 0.5%, and cultivating pH is 6～8, and culture temperature is 38～45 ℃, and incubation time is 2～5 days; Inoculum size was 0.5%～1% when the actinomycetes one-level was cultivated, and is preferred 0.5%, and cultivating pH is 6～8, and culture temperature is 27～32 ℃, and incubation time is 5～7 days.
6. the preparation method of microbial fertilizer according to claim 4, it is characterized in that, the photosynthetic bacterium secondary is cultivated and is preferably the intermittent type aerated culture in the described step (2), inoculum size is 5%～15%, preferred 10%, cultivating pH is 6～10, and culture temperature is 28～36 ℃, intensity of illumination is 3000～4000 luxs, and incubation time is 7～10 days; The subtilis secondary is cultivated and to be preferably aerated culture, and inoculum size is 10%～20%, and is preferred 10%, and cultivating pH is 6～8, and culture temperature is 28～32 ℃, and incubation time is 2～5 days; The yeast secondary is cultivated and to be preferably aerated culture, and inoculum size is 15%～35%, and is preferred 10%, and cultivating pH is 4～7, and culture temperature is 25～30 ℃, and incubation time is 1～2 day; The bacillus acidocldarius secondary is cultivated and to be preferably aerated culture, and inoculum size is 25%～45%, and is preferred 10%, and cultivating pH is 6～8, and culture temperature is 38～45 ℃, and incubation time is 2～5 days; The actinomycetes secondary is cultivated and to be preferably aerated culture, and inoculum size is 3%～15%, and is preferred 10%, and cultivating pH is 6～8, and culture temperature is 27～32 ℃, and incubation time is 5～7 days.
7. the application of the arbitrary described microbial fertilizer of claim 1～3 aspect the resistance that improves seed, pre-preventing disease and pest, purification water quality of paddy field, raising rice yield.
8. the arbitrary described microbial fertilizer of claim 1～3 is being accelerated the application of rice stubble aspect becoming thoroughly decomposed.
9. the using method of the arbitrary described microbial fertilizer of claim 1～3, it is characterized in that in paddy rice seed soaking phase, nursery stage, vegetative period, add described microbial fertilizer when soaking root, when rice stubble becomes thoroughly decomposed in proportion, omnidistance result of use is more obvious.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102172145A (en) * | 2011-02-14 | 2011-09-07 | 天津师范大学 | Application of waste compost microbial agent to improving salt resistance of lawn grass |
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| CN104488625A (en) * | 2014-11-29 | 2015-04-08 | 张旭 | Planting method of selenium enriched rice |
| CN104488625B (en) * | 2014-11-29 | 2016-06-15 | 张旭 | A kind of implantation methods of Selenium rich rice |
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| CN110150546A (en) * | 2019-05-14 | 2019-08-23 | 江苏艾津农业科技服务有限公司 | A kind of production method of high-quality food flavor rice |
| CN112544374A (en) * | 2019-09-25 | 2021-03-26 | 地角黄土亲环境营农组合法人 | Environment-friendly cultivation method of GABA (gamma-aminobutyric acid) rice with effects of reducing blood sugar and preventing dementia |
| CN113545263A (en) * | 2021-07-28 | 2021-10-26 | 青岛万慧源环保科技有限公司 | Method for increasing yield of rice in saline-alkali soil |
| CN115669682A (en) * | 2021-07-30 | 2023-02-03 | 宝钢金属有限公司 | Direct-seeding Zhanshui rice seed coating agent containing reduced iron powder, coating method and application thereof |
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