CN101786914B - Microorganism fertilizer for promoting rice production, preparation method and application thereof - Google Patents
Microorganism fertilizer for promoting rice production, preparation method and application thereof Download PDFInfo
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Abstract
The invention also relates to a microorganism fertilizer. The composite microorganism preparation comprises the components based on the volume ratio: 5-15% of photosynthetic bacteria, 10-20% of bacillus subtilis, 15-35% of saccharomycete, 25-45% of thermophilic bacillus and 3-15% of actinomycete. The invention also relates to the application of the microorganism fertilizer in the aspects of improving the resistance of seeds, preventing plant diseases and insect pests, purifying the water quality of rice field and increasing the rice production, and a using method thereof. The invention also relates to the application of the microorganism fertilizer in the aspect of accelerating rice stubble rotten degree, and a using method thereof.
Description
Technical field
The invention belongs to fertilizer field, be specifically related to a kind of microbial fertilizer application aspect Rice Cropping and preparation method thereof.
Background technology
Bread is the staff of life, and food be take rice as first.Paddy rice, approximately 4.2 hundred million mu of the total sown areas of China, account for 30% of the total sown area of food crop, and output has accounted for 40% of total output of grain, is the first food crop of China.Although successively by the improvement of seed variety, realized the secondary leap of output, the quality of depending merely on seed improves quality and raising output is inadequate, how to improve depend on for existence the quality of soil of seed and is only more crucial factor.Rice terrace is excessively used chemical fertilizer at present, and the use of ignoring fertilizer, microbial-bacterial fertilizer causes soil compaction, and air permeability is poor, causes root system development bad; Paddy rice first crop process of growth produces affects root system development from poisonous substance matter; Paddy rice rice stubble rots process because lacking corresponding microorganism, and the speed of rotting is slow, produces objectionable impurities many, affects Root Growth of Rice; Use that weedicide is improper, herbicide damage, fertilizer damage be serious.These phenomenons show to only have improvement soil, and elimination is hardened, and eliminate the toxic substance in soil, and the beneficial microorganism and the organic matter that increase in soil just can impel paddy rice significantly to increase production.Realize the dual leap of rice quality and output.
Summary of the invention
The object of this invention is to provide a kind of microbial fertilizer that is applied to paddy rice aspect.
The present invention also aims to provide the preparation method of this microbial fertilizer.
The present invention also aims to provide the application of this microbial fertilizer preparation aspect pre-preventing disease and pest, purification water quality of paddy field, raising rice yield.
The present invention also aims to provide this microbial fertilizer preparation accelerating the application of rice stubble aspect becoming thoroughly decomposed.
The present invention also aims to provide the using method of this microbial fertilizer.
The invention provides a kind of microbial fertilizer, is to be mixed by photosynthetic bacterium, subtilis, yeast, bacillus acidocldarius, actinomycetic zymocyte liquid.Wherein, the volume of described each zymocyte liquid by volume part meter be preferably: photosynthetic bacterium 5～15%, subtilis 10～20%, yeast 15～35%, bacillus acidocldarius 25～45%, actinomycetes 3～15%.
In various zymocyte liquids, unit living bacteria count is not less than 500,000,000/milliliter.
Wherein said photosynthetic bacterium is preferably swamp Rhodopseudomonas (Rhodopseudomonaspalustris); Described subtilis (Bacillus subtilis); Described yeast is preferably cereuisiae fermentum (Saccharomyces cerevisiae); Described bacillus acidocldarius is bacstearothermophilus (Bacillus thermophilus); Described actinomycetes are preferably Jingyang streptomycete (Streptomyces jingyangesis).
The present invention also provides the preparation method of described microbial fertilizer.This preparation method comprises the steps:
(1) respectively photosynthetic bacterium, subtilis, yeast, bacillus acidocldarius, actinomycetic female kind are inoculated in to one-level substratum and carry out one-level cultivation, obtain first class inoculum;
(2) respectively photosynthetic bacterium, subtilis, yeast, bacillus acidocldarius, actinomycetic first class inoculum are inoculated in to secondary medium and carry out secondary cultivation, obtain zymocyte liquid;
(3) each zymocyte liquid is mixed in proportion and obtains composite bacteria.
Wherein, when photosynthetic bacterium one-level is cultivated in step (1), inoculum size is 0.5%～1%, preferably 0.5%, and cultivating pH is 6～10, and culture temperature is 28～36 ℃, and intensity of illumination is 3000～4000 luxs, and incubation time is 7～10 days; When subtilis one-level is cultivated, inoculum size is 0.5%～1%, preferably 0.5%, and cultivating pH is 6～8, and culture temperature is 28～34 ℃, and incubation time is 2～3 days; When yeast one-level is cultivated, inoculum size is 0.5%～1%, preferably 0.5%, and cultivating pH is 4～7, and culture temperature is 25～30 ℃, and incubation time is 1～2 day; When bacillus acidocldarius one-level is cultivated, inoculum size is 0.5%～1%, preferably 0.5%, and cultivating pH is 6～8, and culture temperature is 38～45 ℃, and incubation time is 2～5 days; When actinomycetes one-level is cultivated, inoculum size is 0.5%～1%, preferably 0.5%, and cultivating pH is 6～8, and culture temperature is 27～32 ℃, and incubation time is 5～7 days;
Photosynthetic bacterium secondary is cultivated and is preferably intermittent type aerated culture in step (2), and inoculum size is 5%～15%, and preferably 10%, cultivating pH is 6～10, and culture temperature is 28～36 ℃, and intensity of illumination is 3000～4000 luxs, and incubation time is 7～10 days; Subtilis secondary is cultivated and to be preferably aerated culture, and inoculum size is 5%～15%, and preferably 10%, cultivating pH is 6～8, and culture temperature is 28～32 ℃, and incubation time is 2～5 days; Yeast secondary is cultivated and to be preferably aerated culture, and inoculum size is 5%～15%, and preferably 10%, cultivating pH is 4～7, and culture temperature is 25～30 ℃, and incubation time is 1～2 day; Bacillus acidocldarius secondary is cultivated and to be preferably aerated culture, and inoculum size is 5%～15%, and preferably 10%, cultivating pH is 6～8, and culture temperature is 38～45 ℃, and incubation time is 2～5 days; Actinomycetes secondary is cultivated and to be preferably aerated culture, and inoculum size is 5%～15%, and preferably 10%, cultivating pH is 6～8, and culture temperature is 27～32 ℃, and incubation time is 5～7 days.
The present invention also provides this microbial fertilizer in preparation prevention rice pest, the application that purify water quality of paddy field, improves rice yield aspect simultaneously.
The present invention also aims to provide described microbial fertilizer to accelerate the application in rice stubble digest process in preparation.
The present invention also provides the using method of described microbial fertilizer, it is characterized in that adding in proportion described microbial fertilizer when rice seed soaking, nursery stage and vegetative period, harvesting aftertreatment rice stubble, in seed soaking, spray the use when filling with root.
Microbial fertilizer of the present invention provides a kind of can carry out after presoaking and germinating at rice paddy seed, can break fast seed dormancy, promote seed germination, strengthen seed resistance, for guaranteeing that full stand, strong sprout lay a solid foundation, for preventing and treating disease, suppressing the generation of insect pest, the resistance of raising crop etc. have played good effect, be the microbial fertilizer of an economical and effective well stimulation simultaneously.In rice field, apply after the present invention, can P and K decomposing fixed nitrogen, and change the trace element in soil, can produce various plants mediator agent, greatly change crop microcirculating state, promote the absorption of nutrient and conversion, strengthen photosynthesis, accelerate cell fission speed, accelerate cell and expand, promote growth, improve output, improve quality, there is sterilization simultaneously and press down worm effect, for common Achlya spp disease, bakanae disease, banded sclerotial blight, rice blast, all produce effect.Microbial fertilizer of the present invention, state of the art is reached advanced world standards, is by multiple beneficial living microorganism process scientific matching, is jointly composited, and has really reached pleiotropy, and unit living bacteria count is not less than 2,000,000,000/milliliter, considerably beyond national standard.
Useful technique effect and using method are embodied in following several aspect:
With after the present invention's seed soaking, can break fast seed dormancy, promote seed germination, strengthen seed resistance, guarantee that the neat seedling of seedling is strong, seedling rate is high; Effectively improve soil physical and chemical property, prevent soil belt poison, prevent and treat the generation of soil-borne disease, Achlya spp disease, bakanae disease, banded sclerotial blight, rice blast; Impel root growth, main root is sturdy, and fibrous root is many; Cold-resistant drought-resistance ability strengthens; Purify water, blue bath obviously tails off; Short control combination, steadily and surely grows paddy rice, strengthens photosynthesis, and short root increases tiller, makes the early growth and quick tiller of tillering, and reaches that seedling is strong, fringe is many, fringe is large, prevents remaining green when it is due to become yellow and ripe late-maturing and de-fertile early ageing; Improve output 17% left and right, improvement quality.
After becoming thoroughly decomposed with microbial fertilizer processing rice stubble of the present invention, can accelerate rice stubble decomposition rate, reduce obnoxious flavour and discharge, decompose paddy rice from poisonous substance matter, solve paddy rice continuous cropping; Increase soil beneficial microbe colony, suppress soil disease and occur, improve rice shoot survival rate, strong rice root; Purifying water body, reduces the harmful organism growths such as blue-green algae, builds ecological water body, creates paddy rice healthy growth environment; Improve utilization rate of fertilizer, promote paddy growth, increase rice yield, improve rice quality.
Accompanying drawing explanation (in figure " demonstration group " for having used design sketch of the present invention, " control group " be not for being used design sketch of the present invention)
Fig. 1 microbial inoculum production technological process.
Fig. 2 has been used the present invention and has not used the comparison diagram in seedling stage of the present invention, and left side is used that rice seedling rate of the present invention is high, seedling is neat, seedling is strong, leaf color jade green, well developed root system, strong resistance; Right side is not used that seedling rate of the present invention is low, uneven, leaf look yellowish green.
Fig. 3-1 has been used rice root fibrous root of the present invention white, strong, long, and vitality is strong, can remain vigorous dietetic alimentation ability, and after root system field-transplanting like this, seedling-slowing stage is short according to experts' analysis;
Fig. 3-2 are not used paddy rice fibrous root of the present invention short, thin, in vain must be few, and the ability that most of root system has been described or has lost gradually absorption nutrient is and withered, and after this type of root system field-transplanting of analysis expert, transplanting seedling time is longer.
Rice field of the present invention was used in Fig. 4-1, and field blue-green algae material is few, and dry weight is on average 0.305kg/m
2, and all atrophys.
Rice field of the present invention is not used in Fig. 4-2, and field blue-green algae material is many, and average dry weight is 3.02kg/m
2.
It is high that Fig. 5-1 is used paddy rice the mean tillering rate of the present invention not use, and cane is sturdy, healthy.
Fig. 5-2 are not used rice tillering of the present invention less, and cane has canescence mould.
Fig. 6-1 use paddy rice arrow leaf of the present invention is large, photosynthetic by force, heading is neat, growing way is good.
Fig. 6-2 do not use paddy rice early ageing of the present invention serious, ear irregular, rice blast big area occurs, a little less than growing way.
Embodiment
Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.
The substratum of each bacterial classification
The one-level substratum of swamp Rhodopseudomonas is: MgSO
47H
2o 0.2g, (NH
4)
2sO
41.0g, NaHCO
35.0g, K
2hPO
40.5g, NaCl 0.2g, peptone 1.5g; Each composition is dissolved in 400ml distilled water, uses H
3pO
4and Na
2cO
3adjust pH 7.1, then add distilled water to 1000ml, 115 ℃ of sterilizings 10 minutes; Secondary medium is: Na
2cO
33g, NaCl 0.1g, (NH
4)
2sO
40.3g, MgSO
40.2g, KH
2pO
30.5g, K
2hPO
30.3g, ferric ammonium citrate 7ml, peptone 1.2g, with distilled water, be settled to 1000ml, use H
3pO
4and Na
2cO
3adjusting pH is 7.2.
The one-level substratum of subtilis is: extractum carnis 0.3%, peptone 1.0%, NaCl 0.5%, with distilled water, be settled to 1000ml, and pH 7.0; Secondary medium is: extractum carnis 1.0%, peptone 1.0%, NaCl 0.5%, agar 2.0%, be settled to 1000ml with distilled water, pH7.0.
The one-level substratum of cereuisiae fermentum is: potato juice 800ml, glucose 20g, agar 20g, with distilled water, be settled to 1000ml, and pH 6.0; Secondary medium: yeast extract paste 1%, peptone 2%, glucose 2%, MgSO
40.06%, K
2hPO
40.25%, water is supplied 100%, pH6.0.
The one-level substratum of bacstearothermophilus is: extractum carnis 0.3%, peptone 1.0%, NaCl 0.5%, with distilled water, be settled to 1000ml, and pH 7.0; Secondary medium is: extractum carnis 1.0%, peptone 1.0%, NaCl 0.5%, agar 2.0%, be settled to 1000ml with distilled water, pH7.0.
The one-level substratum of Jingyang streptomycete is: KNO
31g, Zulkovsky starch 20g, K
2hPO
40.5g, MgSO
40.5g, NaCl 0.5g, FeSO
40.01g, agar 20g, is settled to 1000ml with distilled water, pH7.2; Secondary medium is KNO
30.8g, Zulkovsky starch 8g, glucose 5g, yeast water 5g, K
2hPO
40.3g, MgSO
40.2g, NaCl 0.4g, FeSO
40.05g, is settled to 1000ml with distilled water, pH7.2.
The preparation method of embodiment 1 complex microorganism preparations
(1) get each 3L of eggplant bottle bacterium of swamp Rhodopseudomonas, subtilis, cereuisiae fermentum, bacstearothermophilus and Jingyang streptomycete, be inoculated in respectively in the one-level substratum of each bacterial classification of 300L and carry out one-level cultivation, obtain the first class inoculum of each component;
(2) get each 300L of first class inoculum of swamp Rhodopseudomonas, subtilis, cereuisiae fermentum, bacstearothermophilus and Jingyang streptomycete, be inoculated in respectively in the secondary medium of each bacterial classification of 3000L and carry out secondary cultivation, obtain the zymocyte liquid of each component;
(3) zymocyte liquid of each component is mixed and carries out compoundly in the ratio of swamp Rhodopseudomonas 12%, subtilis 10%, cereuisiae fermentum 30%, bacstearothermophilus 43%, Jingyang streptomycete 5% in 10000L container for storing liquid, obtain 10000L composite bacteria.
In step (2), swamp Rhodopseudomonas is intermittent type aerated culture; Subtilis is aerated culture; Cereuisiae fermentum is aerated culture; Bacstearothermophilus is aerated culture; Jingyang streptomycete is aerated culture.
The preparation method of embodiment 2 complex microorganism preparations
(1) get each 5L of eggplant bottle bacterium of swamp Rhodopseudomonas, subtilis, cereuisiae fermentum, bacstearothermophilus and Jingyang streptomycete, be inoculated in respectively in the one-level substratum of each bacterial classification of 500L and carry out one-level cultivation, obtain the first class inoculum of each component;
(2) get each 500L of first class inoculum of swamp Rhodopseudomonas, subtilis, cereuisiae fermentum, bacstearothermophilus and Jingyang streptomycete, be inoculated in respectively in the secondary medium of each bacterial classification of 5000L and carry out secondary cultivation, obtain the zymocyte liquid of each component;
(3) zymocyte liquid of each component is mixed and carries out compoundly in the ratio of swamp Rhodopseudomonas 5%, subtilis 15%, cereuisiae fermentum 30%, bacstearothermophilus 40%, Jingyang streptomycete 10% in 10000L container for storing liquid, obtain 10000L composite bacteria.
In step (2), swamp Rhodopseudomonas is intermittent type aerated culture; Subtilis is aerated culture; Cereuisiae fermentum is aerated culture; Bacstearothermophilus is aerated culture; Jingyang streptomycete is aerated culture.
The preparation method of embodiment 3 complex microorganism preparations
(1) get each 6L of eggplant bottle bacterium of swamp Rhodopseudomonas, subtilis, cereuisiae fermentum, bacstearothermophilus and Jingyang streptomycete, be inoculated in respectively in the one-level substratum of each bacterial classification of 600L and carry out one-level cultivation, obtain the first class inoculum of each component;
(2) get each 600L of first class inoculum of swamp Rhodopseudomonas, subtilis, cereuisiae fermentum, bacstearothermophilus and Jingyang streptomycete, be inoculated in respectively in the secondary medium of each bacterial classification of 6000L and carry out secondary cultivation, obtain the zymocyte liquid of each component;
(3) zymocyte liquid of each component is mixed and carries out compoundly in the ratio of swamp Rhodopseudomonas 8%, subtilis 12%, cereuisiae fermentum 35%, bacstearothermophilus 42%, Jingyang streptomycete 3% in 10000L container for storing liquid, obtain 10000L composite bacteria.
In step (2), swamp Rhodopseudomonas is intermittent type aerated culture; Subtilis is aerated culture; Cereuisiae fermentum is aerated culture; Bacstearothermophilus is aerated culture; Jingyang streptomycete is aerated culture.
The omnidistance using method of embodiment 4 paddy growths and effect
1. presoaking and germinating: with 50-100 times of diluent seed soaking, can break fast seed dormancy, promote seed germination, strengthen seed resistance.
2. culturing and transplanting seedlings: 7-8 days before transplanting, 300 times of diluents spray, and guarantee that the neat seedling of seedling is strong, and seedling rate is high.
3. before rice transplanting, with water, fill with: diluent of the present invention is evenly sprayed in rice field, mu consumption 1000-2000ml, can effectively improve soil physical and chemical property, prevent soil belt poison, prevent and treat the generation of soil-borne disease, prevention of water seedling blight of rice disease, bakanae disease, banded sclerotial blight, rice blast; Purify water, blue bath obviously tails off.
4. jointing stage, tillering phase, booting filling stage, respectively with 600 times of diluent foliage spray, are impelled root growth, and main root is sturdy, and fibrous root is many; Cold-resistant drought-resistance ability strengthens.
Omnidistance with after the present invention, short control combination, steadily and surely grows paddy rice, strengthens photosynthesis, and short root increases tiller, makes the early growth and quick tiller of tillering, and reaches that seedling is strong, fringe is many, fringe is large, prevents remaining green when it is due to become yellow and ripe late-maturing and de-fertile early ageing; Improve output 17% left and right, improvement quality.
Embodiment 5 accelerates using method and the effect that rice stubble becomes thoroughly decomposed
Before soil is about to turn over, microbial fertilizer of the present invention is evenly sprayed onto in rice terrace to 2000 milliliters/mu; Or before turning over, along with irrigating, microbial inoculum punching is executed in rice terrace to 2000 milliliters/mu.Can accelerate rice stubble decomposition rate, reduce obnoxious flavour and discharge, decompose paddy rice from poisonous substance matter, solve paddy rice continuous cropping; Increase soil beneficial microbe colony, suppress soil disease and occur, improve rice shoot survival rate, strong rice root; Purifying water body, reduces the harmful organism growths such as blue-green algae, builds ecological water body, creates paddy rice healthy growth environment; Improve utilization rate of fertilizer, promote paddy growth, increase rice yield, improve rice quality.
Precaution during embodiment 6 is used
1, forbid mixed with any medicine.
2, first slowly unscrew bottle cap during uncork and discharge shielding gas, prevent that bacterium hydrorrhea from going out.
3, normal temperature lucifuge sealed type storage, with front shaking up.
4, diluting water wants clean, and water temperature is not higher than 40 ℃.
5, container used, equipment must clean up.
6, this product safety, nontoxic, have no side effect.
Experimental example 1: the application of microbial preparation of the present invention aspect paddy rice
Nan Mazhuan village, Lankao, Henan is the best place of rice cultivation always.2006, on the paddy rice of the Nan Ma of the present invention village, be widely used, and obtained unusual effect, be embodied in: soil has obtained improvement, seedling rate is high, Miao Qi, Miao Zhuan (as Fig. 2), well developed root system is (as Fig. 3-1, 3-2), striped blight and planthopper harm have effectively been controlled, make thousand seed weight increase 0.7g, the average yield per mu of control group is 396.65kg, and the average yield per mu of demonstration group is 466.55kg, than control group mu volume increase 69.9kg, output improves 17%, the omnidistance usage quantity that reduces by 30% fertilizer and pesticide, rice milling yield is high and rice matter is good, and purchasing in the hearing of rice bag ground price of Held in Beijing, won expert, professor's a consistent favorable comment!
Experimental example 2: the application of microbial preparation of the present invention aspect paddy rice
Big area blue-green alga bloom has been broken out in Nan Mazhuan paddy fields, Lankao, Henan in June, 2007, company to part peasant household paddy field application by throwing them after the present invention, make the blue-green algae in rice terrace controlled.Breaking out the rice terrace of blue-green algae and using in rice terrace of the present invention, choose at random the plot of a square metre, with grate, fish for the suspended biological (comprising blue-green algae) in water, after taking-up, be placed on after hanging drip-dry moisture in the string bag and weigh: using field of the present invention blue-green algae material is 0.305kg (mean value), do not use rice field of the present invention, field blue-green algae material 3.02kg, and used the whole atrophys of blue-green algae of the present invention, water quality limpid (as Fig. 4-1,4-2).
Experimental example 3: the application of microbial preparation of the present invention aspect paddy rice
2008, Jiangsu Province's Yancheng City, scientific research, popularization, master of instruction carry out the on-the-spot product of surveying to Xin Yang farm to using paddy rice of the present invention " Wu-Yu-Geng 3 ": under same medium soil fertility condition on the lower side, the in the situation that of 5 kilograms of tillering fertilizer and every mu of average Shaoshis of ear manuer, use after the comprehensive treating processs such as microbial inoculum is soaked seed, repair in seedbed, filling root, foliage-spray, do not use rice tillering of the present invention less, and cane have canescence mould; Use paddy rice figure the mean tillering rate of the present invention many compared with control group, and cane is sturdy, health (as Fig. 5-1,5-2).Use every mu of number of productive ear of the present invention to reach 23.09 ten thousand, than untapped, increased by 18.28%, every mu of per unit area yield is up to 678.54 kilograms, than untapped volume increase 15.47%.Expert conclusion: Rice Production is used this novel " microbial inoculum ", does not only increase user's cost, and drops into and reduce, and output increases, and abridged edition increases income significantly.
Experimental example 4: the application of microbial preparation of the present invention aspect paddy rice
Qiyang,hunan red soil experiment centre: sum up use paddy rice arrow leaf of the present invention large, photosynthetic strong, heading is neat, growing way is good, do not use paddy rice early ageing of the present invention serious, ear irregular, rice blast big area occurs, growing way weak (as Fig. 6-1,6-2).Use rice field of the present invention to promote the utilization ratio of soil phosphorus, potassium, significantly increased that paddy rice effective fringe, grain number per spike, the real grain of fringe are heavy, thousand seed weight, reduce paddy rice ghost rate, volume increase 10%-15%.
Claims (5)
1. a microbial fertilizer, is characterized in that, is to be mixed by photosynthetic bacterium, subtilis, yeast, bacillus acidocldarius and actinomycetic zymocyte liquid;
The volume of described each zymocyte liquid by volume part is counted: photosynthetic bacterium 5～15%, subtilis 10～20%, yeast 15～35%, bacillus acidocldarius 25～45%, actinomycetes 3～15%;
Described photosynthetic bacterium is swamp Rhodopseudomonas; Described yeast is cereuisiae fermentum; Described bacillus acidocldarius is bacstearothermophilus; Described actinomycetes are Jingyang streptomycete.
2. a preparation method for microbial fertilizer described in claim 1, the method comprises the steps:
(1) respectively photosynthetic bacterium, subtilis, yeast, bacillus acidocldarius, actinomycetic female kind are inoculated in to one-level substratum and carry out one-level cultivation, obtain first class inoculum;
(2) respectively photosynthetic bacterium, subtilis, yeast, bacillus acidocldarius, actinomycetic first class inoculum are inoculated in to secondary medium and carry out secondary cultivation, obtain zymocyte liquid;
(3) each zymocyte liquid is mixed in proportion;
When in described step (1), photosynthetic bacterium one-level is cultivated, inoculum size counts 0.5%～1% by volume, and cultivating pH is 6～10, and culture temperature is 28～36 ℃, and intensity of illumination is 3000～4000 luxs, and incubation time is 7～10 days; When subtilis one-level is cultivated, inoculum size counts 0.5%～1% by volume, and cultivating pH is 6～8, and culture temperature is 28～34 ℃, and incubation time is 2～3 days; When yeast one-level is cultivated, inoculum size counts 0.5%～1% by volume, and cultivating pH is 4～7, and culture temperature is 25～30 ℃, and incubation time is 1～2 day; When bacillus acidocldarius one-level is cultivated, inoculum size counts 0.5%～1% by volume, and cultivating pH is 6～8, and culture temperature is 38～45 ℃, and incubation time is 2～5 days; When actinomycetes one-level is cultivated, inoculum size counts 0.5%～1% by volume, and cultivating pH is 6～8, and culture temperature is 27～32 ℃, and incubation time is 5～7 days; In described step (2), photosynthetic bacterium secondary is cultivated as intermittent type aerated culture, and inoculum size counts 5%～15% by volume, and cultivating pH is 6～10, and culture temperature is 28～36 ℃, and intensity of illumination is 3000～4000 luxs, and incubation time is 7～10 days; Subtilis secondary is cultivated as aerated culture, and inoculum size counts 5%～15% by volume, and cultivating pH is 6～8, and culture temperature is 28～32 ℃, and incubation time is 2～5 days; Yeast secondary is cultivated as aerated culture, and inoculum size counts 5%～15% by volume, and cultivating pH is 4～7, and culture temperature is 25～30 ℃, and incubation time is 1～2 day; Bacillus acidocldarius secondary is cultivated as aerated culture, and inoculum size counts 5%～15% by volume, and cultivating pH is 6～8, and culture temperature is 38～45 ℃, and incubation time is 2～5 days; Actinomycetes secondary is cultivated as aerated culture, and inoculum size counts 5%～15% by volume, and cultivating pH is 6～8, and culture temperature is 27～32 ℃, and incubation time is 5～7 days.
3. the preparation method of microbial fertilizer according to claim 2, is characterized in that, when in described step (1), photosynthetic bacterium one-level is cultivated, inoculum size counts 0.5% by volume; When subtilis one-level is cultivated, inoculum size counts 0.5% by volume; When yeast one-level is cultivated, inoculum size counts 0.5% by volume; When bacillus acidocldarius one-level is cultivated, inoculum size counts 0.5% by volume; When actinomycetes one-level is cultivated, inoculum size counts 0.5% by volume.
4. the preparation method of microbial fertilizer according to claim 2, is characterized in that, in described step (2), photosynthetic bacterium secondary is cultivated inoculum size and counted by volume 10%; Subtilis secondary is cultivated inoculum size and is counted by volume 10%; Yeast secondary is cultivated inoculum size and is counted by volume 10%; Bacillus acidocldarius secondary is cultivated inoculum size and is counted by volume 10%; Actinomycetes secondary is cultivated inoculum size and is counted by volume 10%.
5. the application of microbial fertilizer claimed in claim 1 aspect the resistance of raising seed, pre-preventing disease and pest, purification water quality of paddy field, raising rice yield.
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| CN102153402A (en) * | 2010-12-15 | 2011-08-17 | 沈阳科丰牧业科技有限公司 | Liquid complex microorganism bacterial manure and preparation method thereof |
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