CN102153402A - Liquid complex microorganism bacterial manure and preparation method thereof - Google Patents
Liquid complex microorganism bacterial manure and preparation method thereof Download PDFInfo
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- CN102153402A CN102153402A CN2010105884852A CN201010588485A CN102153402A CN 102153402 A CN102153402 A CN 102153402A CN 2010105884852 A CN2010105884852 A CN 2010105884852A CN 201010588485 A CN201010588485 A CN 201010588485A CN 102153402 A CN102153402 A CN 102153402A
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Abstract
The invention belongs to a crop fertilizer, in particular to a liquid complex microorganism bacterial manure and a preparation method thereof. The liquid complex microorganism bacterial manure is prepared by mixing fermentations of 10-30wt% of Azotobocter chroococcum, 10-20wt% of phosphate-decomposing bacillus megaterium, 10-20wt% of silicatic bacteria, 10-30wt% of Streptomyces jingyangensis and 10-20wt% of Photosynthetic bacteria Rhodomeudomonas palustris as well as trace elements accounting for 30-40% of total mass of the above mixed fermentations. The microorganism components in the liquid complex microorganism bacterial manure can activate soil and release nutrients hard to adsorb and utilize by plants in soil so as to inhibit the possibility of soil-borne disease and prevent bacterial diseases from intruding, and the components of trace elements can be used for supplementing the trace elements required by plants. According to the liquid complex microorganism bacterial manure and the preparation method, the quality and the yield of the crop can be obviously improved.
Description
Technical field
The invention belongs to farm crop fertilizer, specifically a kind of liquid composite microbic bacterial fertilizer and preparation method thereof.
Background technology
Using in a large number for a long time of inorganic fertilizer also brought some negative impacts when improving crop yield.In recent years because the developing rapidly of chemical fertilizer industry, a large amount of chemical fertilizer is applied in the soil, though the mineral manure fertilizer efficiency is more obvious, but because the contained inorganic nutrients in chemical fertilizer the inside is fixed by one-tenth branch such as lime contained in the soil easily, cause soil compaction, and a large amount of uses of upstream chemical fertilizer can cause the pollution of downstream river, cause the waste of the energy and the pollution of environment, cause whole farmland ecological environment and people's living environment all to be subjected to serious threat.And, in the chemical fertilizers production process, can produce some poisonous impurity, such as cadmium etc., have a strong impact on human beings'health.Meanwhile, the excessive chemical fertilizer of using also can directly influence quality of agricultural product, has reduced the market competitiveness of agricultural-food.Expose at chemical fertilizer under the background of many drawbacks, organic fertilizer is subjected to people's attention day by day, but because animal epidemic frequent causes containing in its ight soil a lot of harmful germs, apply and to cause crop that disease takes place in the soil, therefore, be used for reducing inorganic fertilizer and use by using making of a kind of new-type fertilizer, and improve the organic chemical fertilizer quality and be very important.
Summary of the invention
The object of the invention is to provide a kind of liquid composite microbic bacterial fertilizer and preparation method thereof.
For achieving the above object, the technical solution used in the present invention is:
A kind of liquid composite microbic bacterial fertilizer: by weight percentage, the fermented product of 10-30% azotobacter chroococcum, 10-20% phosphorus decomposing bacillus megaterium, 10-20% silicate bacteria, 10-30% Jingyang streptomycete and 10-20% light and bacterium swamp Rhodopseudomonas mixes, and the trace element of above-mentioned mixed fermentation thing total mass 30-40%.
Described trace element is micro-borax, CuSO
4, ZnCl
2, MnSO
4.3H
2O, (NH
4) Mo
4And FeSO
4, add-on is respectively 2%, 4 %, 3 %, 7%, 10%, 10% of fermented product total mass.
The preparation method of liquid composite microbic bacterial fertilizer: azotobacter chroococcum, phosphorus decomposing bacillus megaterium, silicate bacteria, Jingyang streptomycete and light and bacterium swamp Rhodopseudomonas are cultured to logarithmic phase respectively in liquid nutrient medium separately, with tunning by weight percentage, 10-30% azotobacter chroococcum, 10-20% phosphorus decomposing bacillus megaterium, 10-20% silicate bacteria, 10-30% Jingyang streptomycete and 10-20% light and bacterium swamp Rhodopseudomonas fermented product mix mixed viable count 〉=2 * 10
8Individual, and the trace element of above-mentioned fermented product total mass 30-40%.
Described azotobacter chroococcum: azotobacter chroococcum is inoculated in the fermented liquid under aerobic environment 25-30 ℃ of fermentation 48-72 hour by 3-5%, stand-by; Fermented liquid is 3.6 ‰ wheat brans, 0.02 ‰ K by weight
2HPO
4, 0.01 ‰ MgSO
47H
2O, 3 ‰ (NH
4)
2HPO
4, surplus is a 1000ml distilled water;
The phosphorus decomposing bacillus megaterium: the phosphorus decomposing bacillus megaterium is inoculated in the fermented liquid under aerobic environment 25-30 ℃ of fermentation 20-40 hour by 3-5%, stand-by; Fermented liquid is by weight, and peptone 5 ‰, extractum carnis 3 ‰, sodium-chlor 5 ‰, PH are about 7.0, surplus is a 1000ml distilled water;
Silicate bacteria is inoculated in the fermented liquid under aerobic environment 25-30 ℃ of fermentation 72-90 hour with silicate bacteria by 3-5%, and is stand-by; Fermented liquid is 5 ‰ sucrose, 2 ‰ Na by weight
2HPO
4, 0.5 ‰ MgSO
47H
2O, 0.005 ‰ FeCl
3, 0.1 ‰ CaCO
3, 1 ‰ glass powder, surplus is a 1000ml distilled water;
Light and bacterium swamp Rhodopseudomonas: light and bacterium swamp Rhodopseudomonas are inoculated in the fermented liquid under aerobic environment 25-30 ℃ of fermentation 72-90 hour by 3-5%, stand-by; Fermented liquid is by weight, 3 ‰ yeast powders, and 3 ‰ peptones, 0.5 ‰ sal epsom, 0.3 ‰ calcium chloride, surplus is a 1000ml distilled water
The Jingyang streptomycete: the Jingyang streptomycete is inoculated in the fermented liquid under aerobic environment 25-30 ℃ of fermentation 72-90 hour by 3-5%, stand-by; Fermented liquid is 1.0 ‰ KNO by weight
3, 0.5 ‰ K
2HPO
4, 0.5 ‰ MgSO
4, 20 ‰ Zulkovsky starches, 0.01 ‰ FeSO
4, surplus is 1000ml distilled water, pH 7.2-7.4.
Described trace element is micro-borax, CuSO
4, ZnCl
2, MnSO
4.3H
2O, (NH
4) Mo
4And FeSO
4, add-on is respectively 2%, 4 %, 3 %, 7%, 10%, 10% of fermented product total mass.
The advantage that the present invention had:
Fertilizer of the present invention is made up of the multiple beneficial microorganism, after the bio-bacterial manure that the present invention is prepared is administered in the soil, can promote soil N, P, K conversion, and then increase soil fertility by increasing the soil organism, reduces the usage quantity of chemical fertilizer; The meta-bolites phytokinin of microorganism can stimulate the growth of crop; The meta-bolites of microorganism (microbiotic) can suppress the harmful microbe breeding, thereby reduce the generation of Plant diseases, simultaneously also can strengthen the plant stress-resistance ability, and then reduce the use of agricultural chemicals, the adding of trace element can replenish the required micro-nutrient of plant.
Composite microbic bacterial fertilizer of the present invention is applicable to all vegetable crops, can obviously improve Soil structure behind the filling root, strengthens soil fertility, reduces the generation of soil-borne disease, improves the vegetable crop quality, increases output.Can make the blade thickening of enlarging afforested area behind the foliage-spray, plant strain growth is vigorous, improves the plant stress-resistance ability, promotes the raising of plant yield and quality, and output improves about 10-25%.
Embodiment
Embodiment 1
Liquid composite microbic bacterial fertilizer: by weight percentage, 10% azotobacter chroococcum, 20% phosphorus decomposing bacillus megaterium, 20% silicate bacteria, 30% Jingyang streptomycete and 20% light and bacterium swamp Rhodopseudomonas mix the fermented product of post-fermentation and culture, and 2% borax, the 4 % CuSO of above-mentioned fermented product total mass
4, 3 % ZnCl
2, 7% MnSO
4.3H
2O, 10% (NH
4) Mo
4, 10% FeSO
4
The preparation method of liquid composite microbic bacterial fertilizer:
Azotobacter chroococcum: azotobacter chroococcum is inoculated in the fermented liquid under aerobic environment 25-30 ℃ of fermentation 48 hours by 3-5%, stand-by; Fermented liquid is 3.6 ‰ wheat brans, 0.02 ‰ K by weight
2HPO
4, 0.01 ‰ MgSO
47H
2O, 3 ‰ (NH
4)
2HPO
4, surplus is a 1000mL distilled water.Described azotobacter chroococcum is numbered 10098 available from Chinese agriculture microbial strains preservation center.
The phosphorus decomposing bacillus megaterium: the phosphorus decomposing bacillus megaterium is inoculated in the fermented liquid under aerobic environment 25-30 ℃ of fermentation 20 hours by 3-5%, stand-by; Fermented liquid is by weight, and peptone 5 ‰, extractum carnis 3 ‰, sodium-chlor 5 ‰, pH are about 7.0, surplus is a 1000ml distilled water; The phosphorus decomposing bacillus megaterium is available from Chinese industrial microbial strains preservation center, and bacterium numbering is 23574.
Silicate bacteria: silicate bacteria is inoculated in the fermented liquid under aerobic environment 25-30 ℃ of fermentation 48 hours by 3-5%, stand-by; Fermented liquid is 5 ‰ sucrose, 2 ‰ Na by weight
2HPO
4, 0.5 ‰ MgSO
47H
2O, 0.005 ‰ FeCl
3, 0.1 ‰ CaCO
3, 1 ‰ glass powder, surplus is a 1000ml distilled water; Silicate bacteria is numbered 23575 available from Chinese industrial microbial strains preservation center.
Light and bacterium swamp Rhodopseudomonas: light and bacterium swamp Rhodopseudomonas are inoculated in the fermented liquid under aerobic environment 25-30 ℃ of fermentation 72 hours by 3-5%, stand-by; Fermented liquid is yeast powder 3 ‰, peptone 3 ‰, MgSO by weight
47H
2O 0. 5 ‰, CaCl
20. 3 ‰, surplus is a 1000mL distilled water, under 25-30 ℃, transfers PH to 6.8-7.0.Light and bacterium swamp Rhodopseudomonas are available from Chinese agriculture microbial strains preservation center, and bacterium numbering is 10649.
The Jingyang streptomycete: the Jingyang streptomycete is inoculated in the fermented liquid under aerobic environment 25-30 ℃ of fermentation 72 hours by 3-5%, stand-by; Fermented liquid is 1.0 ‰ KNO by weight
3, 0.5 ‰ K
2HPO
4, 0.5 ‰ MgSO
4, 20 ‰ Zulkovsky starches, 0.01 ‰ FeSO
4, surplus is 1000ml distilled water, pH 7.2-7.4.The Jingyang streptomycete is 40040 available from Chinese agriculture microbial strains preservation center bacterium numbering.
With bacterial classification after the above-mentioned cultivation by weight percentage, azotobacter chroococcum, phosphorus decomposing bacillus megaterium, silicate bacteria, Jingyang streptomycete and light and bacterium swamp Rhodopseudomonas are cultured to logarithmic phase respectively in liquid nutrient medium separately, with tunning by weight percentage, 10% azotobacter chroococcum, 20% phosphorus decomposing bacillus megaterium, 20% silicate bacteria, 30% Jingyang streptomycete and 20% light and bacterium swamp Rhodopseudomonas mix, and make viable count in every milliliter of mixed solution 〉=2 * 10
8Individual.Add 2% borax, the 4 % CuSO that account for above-mentioned fermented product total mass
4, 3 % ZnCl
2, 7% MnSO
4.3H
2O, 10% (NH
4) Mo
4, 10% FeSO
4, promptly obtain liquid composite microbic bacterial fertilizer.
With the tomato according to the Routine Management cultivation in the greenhouse is subjects, and with the liquid composite microbic bacterial fertilizer of above-mentioned preparation gained, weaker concn is 200 times of liquid, then irritates root and carries out foliage-spray as base manure.The control group that design is not used, 4 repetitions of each treatment group, each repeats 15m
2, the gross weight of tomato in the determination test phase.Test-results shows that the tomato yield of control group is 5.4kg/m
2, the output of test group is 6.6kg/m
2, output has improved 22.2%.
Embodiment 2
Liquid composite microbic bacterial fertilizer: by weight percentage, 30% azotobacter chroococcum, 20% phosphorus decomposing bacillus megaterium, 20% silicate bacteria, 20% Jingyang streptomycete and 10% light and bacterium swamp Rhodopseudomonas mix the fermented product of post-fermentation and culture, and 2% borax, the 4 % CuSO of above-mentioned fermented product total mass
4, 3 % ZnCl
2, 7% MnSO
4.3H
2O, 10% (NH
4) Mo
4, 10% FeSO
4
The preparation method of liquid composite microbic bacterial fertilizer:
Azotobacter chroococcum: azotobacter chroococcum is inoculated in the fermented liquid under aerobic environment 25-30 ℃ of fermentation 48 hours by 3-5%, stand-by; Fermented liquid is 3.6 ‰ wheat brans, 0.02 ‰ K by weight
2HPO
4, 0.01 ‰ MgSO
47H
2O, 3 ‰ (NH
4)
2HPO
4, surplus is a 1000mL distilled water.Described azotobacter chroococcum is numbered 10098 available from Chinese agriculture microbial strains preservation center.
The phosphorus decomposing bacillus megaterium: the phosphorus decomposing bacillus megaterium is inoculated in the fermented liquid under aerobic environment 25-30 ℃ of fermentation 20 hours by 3-5%, stand-by; Fermented liquid is by weight, and peptone 5 ‰, extractum carnis 3 ‰, sodium-chlor 5 ‰, pH are about 7.0, surplus is a 1000ml distilled water; The phosphorus decomposing bacillus megaterium is available from Chinese industrial microbial strains preservation center, and bacterium numbering is 23574.
Silicate bacteria: silicate bacteria is inoculated in the fermented liquid under aerobic environment 25-30 ℃ of fermentation 48 hours by 3-5%, stand-by; Fermented liquid is 5 ‰ sucrose, 2 ‰ Na by weight
2HPO
4, 0.5 ‰ MgSO
47H
2O, 0.005 ‰ FeCl
3, 0.1 ‰ CaCO
3, 1 ‰ glass powder, surplus is a 1000ml distilled water; Silicate bacteria is numbered 23575 available from Chinese industrial microbial strains preservation center.
Light and bacterium swamp Rhodopseudomonas: light and bacterium swamp Rhodopseudomonas are inoculated in the fermented liquid under aerobic environment 25-30 ℃ of fermentation 72 hours by 3-5%, stand-by; Fermented liquid is yeast powder 3 ‰, peptone 3 ‰, MgSO by weight
47H
2O 0. 5 ‰, CaCl
20. 3 ‰, surplus is a 1000mL distilled water, under 25-30 ℃, transfers pH to 6.8-7.0.Light and bacterium swamp Rhodopseudomonas are available from Chinese agriculture microbial strains preservation center, and bacterium numbering is 10649.
The Jingyang streptomycete: the Jingyang streptomycete is inoculated in the fermented liquid under aerobic environment 25-30 ℃ of fermentation 72 hours by 3-5%, stand-by; Fermented liquid is 1.0 ‰ KNO by weight
3, 0.5 ‰ K
2HPO
4, 0.5 ‰ MgSO
4, 20 ‰ Zulkovsky starches, 0.01 ‰ FeSO
4, surplus is 1000ml distilled water, pH 7.2-7.4.
With bacterial classification after the above-mentioned cultivation by weight percentage, 30% azotobacter chroococcum, 20% phosphorus decomposing bacillus megaterium, 20% silicate bacteria, 10% Jingyang streptomycete and 10% light and bacterium swamp Rhodopseudomonas mix, viable count 〉=2 * 10
8Individual fermented product.Add 2% borax, the 4 % CuSO that account for above-mentioned fermented product total mass
4, 3 % ZnCl
2, 7% MnSO
4.3H
2O, 10% (NH
4) Mo
4, 10% FeSO
4, promptly obtain liquid composite microbic bacterial fertilizer.
With the liquid composite microbic bacterial fertilizer of above-mentioned preparation gained, weaker concn is 500 times of liquid, sprays as foliage fertilizer, adds oiliness plant auxiliary agent 20mL/ mu when spraying, to strengthen its adsorptive power on the blade face.
With the cucumber according to the Routine Management cultivation in the greenhouse is subjects, control group that design is not used and the test treatment group of using by such scheme, and 4 repetitions of each treatment group, each repeats 25m
2, measure influence to cucumber yield and quality.Test-results sees table 1 and table 2 for details.
Table 1 is used the influence of composite microbe fertilizer to cucumber yield
| Handle | Output (kg/hm 2） |
| Contrast | 59981 |
| The foregoing description microbial bacteria material | 63276 |
Table 2 is used the influence of composite microbe fertilizer to cucumber quality
| Handle | Vitamin C content (mg/100g) | Soluble sugar (%) |
| Contrast | 21.4 | 3.77 |
| The foregoing description microbial bacteria material | 25.8 | 3.83 |
Claims (5)
1. liquid composite microbic bacterial fertilizer, it is characterized in that: by weight percentage, the fermented product of 10-30% azotobacter chroococcum, 10-20% phosphorus decomposing bacillus megaterium, 10-20% silicate bacteria, 10-30% Jingyang streptomycete and 10-20% light and bacterium swamp Rhodopseudomonas mixes, and the trace element of above-mentioned mixed fermentation thing total mass 30-40%.
2. by the described liquid composite microbic bacterial fertilizer of claim 1, it is characterized in that: described trace element is micro-borax, CuSO
4, ZnCl
2, MnSO
4.3H
2O, (NH
4) Mo
4And FeSO
4, add-on is respectively 2%, 4 %, 3 %, 7%, 10%, 10% of fermented product total mass.
3. the preparation method of the described liquid composite microbic bacterial fertilizer of claim 1, it is characterized in that: azotobacter chroococcum, phosphorus decomposing bacillus megaterium, silicate bacteria, Jingyang streptomycete and light and bacterium swamp Rhodopseudomonas are cultured to logarithmic phase respectively in liquid nutrient medium separately, with tunning by weight percentage, 10-30% azotobacter chroococcum, 10-20% phosphorus decomposing bacillus megaterium, 10-20% silicate bacteria, 10-30% Jingyang streptomycete and 10-20% light and bacterium swamp Rhodopseudomonas fermented product mix mixed viable count 〉=2 * 10
8Individual, and the trace element of above-mentioned fermented product total mass 30-40%.
4. by the preparation method of the described liquid composite microbic bacterial fertilizer of claim 4, it is characterized in that:
Described azotobacter chroococcum: azotobacter chroococcum is inoculated in the fermented liquid under aerobic environment 25-30 ℃ of fermentation 48-72 hour by 3-5%, stand-by; Fermented liquid is 3.6 ‰ wheat brans, 0.02 ‰ K by weight
2HPO
4, 0.01 ‰ MgSO
47H
2O, 3 ‰ (NH
4)
2HPO
4, surplus is a 1000ml distilled water;
The phosphorus decomposing bacillus megaterium: the phosphorus decomposing bacillus megaterium is inoculated in the fermented liquid under aerobic environment 25-30 ℃ of fermentation 20-40 hour by 3-5%, stand-by; Fermented liquid is by weight, and peptone 5 ‰, extractum carnis 3 ‰, sodium-chlor 5 ‰, PH are about 7.0, surplus is a 1000ml distilled water;
Silicate bacteria is inoculated in the fermented liquid under aerobic environment 25-30 ℃ of fermentation 72-90 hour with silicate bacteria by 3-5%, and is stand-by; Fermented liquid is 5 ‰ sucrose, 2 ‰ Na by weight
2HPO
4, 0.5 ‰ MgSO
47H
2O, 0.005 ‰ FeCl
3, 0.1 ‰ CaCO
3, 1 ‰ glass powder, surplus is a 1000ml distilled water;
Light and bacterium swamp Rhodopseudomonas: light and bacterium swamp Rhodopseudomonas are inoculated in the fermented liquid under aerobic environment 25-30 ℃ of fermentation 72-90 hour by 3-5%, stand-by; Fermented liquid is by weight, 3 ‰ yeast powders, and 3 ‰ peptones, 0.5 ‰ sal epsom, 0.3 ‰ calcium chloride, surplus is a 1000ml distilled water;
The Jingyang streptomycete: the Jingyang streptomycete is inoculated in the fermented liquid under aerobic environment 25-30 ℃ of fermentation 72-90 hour by 3-5%, stand-by; Fermented liquid is 1.0 ‰ KNO by weight
3, 0.5 ‰ K
2HPO
4, 0.5 ‰ MgSO
4, 20 ‰ Zulkovsky starches, 0.01 ‰ FeSO
4, surplus is 1000ml distilled water, pH 7.2-7.4.
5. by the preparation method of the described liquid composite microbic bacterial fertilizer of claim 4, it is characterized in that: described trace element is micro-borax, CuSO
4, ZnCl
2, MnSO
4.3H
2O, (NH
4) Mo
4And FeSO
4, add-on is respectively 2%, 4 %, 3 %, 7%, 10%, 10% of fermented product total mass.
Priority Applications (1)
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|---|---|---|---|
| CN2010105884852A CN102153402A (en) | 2010-12-15 | 2010-12-15 | Liquid complex microorganism bacterial manure and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105884852A CN102153402A (en) | 2010-12-15 | 2010-12-15 | Liquid complex microorganism bacterial manure and preparation method thereof |
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|---|---|
| CN102153402A true CN102153402A (en) | 2011-08-17 |
Family
ID=44435129
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| CN2010105884852A Pending CN102153402A (en) | 2010-12-15 | 2010-12-15 | Liquid complex microorganism bacterial manure and preparation method thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102690151A (en) * | 2012-06-28 | 2012-09-26 | 赵鹏 | Composite microbial inoculum organic multielement compound fertilizer and production method thereof |
| CN102951957A (en) * | 2011-08-29 | 2013-03-06 | 保罗生物园科技股份有限公司 | Microbial viable bacterium fruit tree water flush fertilizer, preparation technology and application method thereof |
| CN105060950A (en) * | 2015-04-10 | 2015-11-18 | 上海应用技术学院 | Liquid composite microbial fertilizer and preparation method thereof |
| CN106008101A (en) * | 2016-05-25 | 2016-10-12 | 山西凯盛肥业集团有限公司 | Composite microbial organic fertilizer for saline-alkali soil improvement and preparation method thereof |
| CN111574299A (en) * | 2020-05-28 | 2020-08-25 | 普乐格（厦门）生物科技有限公司 | Bacterial fertilizer for repairing plant leaf antioxidant system and preparation method thereof |
| CN113402327A (en) * | 2021-07-28 | 2021-09-17 | 甘肃省农业科学院植物保护研究所 | Special microbial compound fertilizer for vegetables and preparation method thereof |
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| CN102951957A (en) * | 2011-08-29 | 2013-03-06 | 保罗生物园科技股份有限公司 | Microbial viable bacterium fruit tree water flush fertilizer, preparation technology and application method thereof |
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| CN111574299A (en) * | 2020-05-28 | 2020-08-25 | 普乐格（厦门）生物科技有限公司 | Bacterial fertilizer for repairing plant leaf antioxidant system and preparation method thereof |
| CN113402327A (en) * | 2021-07-28 | 2021-09-17 | 甘肃省农业科学院植物保护研究所 | Special microbial compound fertilizer for vegetables and preparation method thereof |
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Application publication date: 20110817 |